Search Results (40,801)

Objectives: Seawater-immersed burn wounds are highly susceptible to contamination, persistent inflammation, oxidative stress, and delayed healing, while current irrigation solutions remain suboptimal for such acute injuries. This study aimed to evaluate the therapeutic efficacy and underlying mechanisms of plasma-activated water (PAW) as a novel irrigation strategy for these complex wounds. Methods: The antibacterial efficacy of PAW against marine pathogens was first evaluated in vitro. Subsequently, a rat model of seawater-immersed burn injury was established in male Sprague-Dawley (SD) rats to assess the therapeutic effects of PAW irrigation on wound healing, infection control, and underlying biological mechanisms. Results: In vitro, PAW significantly eradicated two major marine pathogens, Vibrio vulnificus and Vibrio parahaemolyticus (p < 0.001). In vivo, PAW markedly accelerated wound closure, achieving complete healing in 23.60 ± 6.50 days vs. 38.67 ± 2.08 days (Normal saline group) and 58.33 ± 10.97 days (Model group) (p < 0.05). PAW significantly reduced bacterial burden, modulated inflammation by decreasing interleukin-6 and increasing interleukin-10, and alleviated oxidative stress, as evidenced by reduced malondialdehyde levels and enhanced superoxide dismutase activity. Histological evaluation demonstrated enhanced re-epithelialization, collagen deposition, and increased expression of vascular endothelial growth factor and platelet endothelial cell adhesion molecule-1. No adverse effects on serum biochemistry or major organ histopathology were observed. Conclusions: PAW may be a safe, promising, and multifunctional irrigation strategy that promotes seawater-immersed burn healing through coordinated antibacterial, anti-inflammatory, antioxidant, and pro-angiogenic effects, highlighting its strong potential for clinical translation. Full article

Antimicrobial resistance (AMR) has become a major concern in the treatment of bacterial infections, and bacteriophage therapy has emerged as a promising alternative to antibiotics. Bacteriophages are highly specific to their bacterial hosts; hence, isolating phages indigenous to a specific region offers a significant advantage against various pathogen strains. We have isolated a cocktail of bacteriophages against pathogenic E. coli from sewage water at a primary healthcare centre. Characterisation of the isolated phages demonstrated their stability across a broad pH and temperature range, strong lytic activity, and effective biofilm degradation, with no cross-reactivity with Staphylococcus aureus (S. aureus). Genomic analysis and phylogenetic studies indicated that the largest phage (by genome size) in the cocktail belongs to the genus Vequintavirus (myoviruses, rV5-like phages), and its genome sequence has been deposited in NCBI (Accession ID: PX741096). The phage genome was linear, with headful (PAC) packaging, encoded lysis proteins, and lacked antibiotic-resistant or major lysogeny-associated genes, collectively suggesting a lytic lifestyle. These findings emphasize the therapeutic potential of rV5-like phages and underscore the critical need to establish phage banks in India to improve disease management. Full article

Recently, Quzhou Aurantii Fructus Extract (QAFE) was reported to exert anti-inflammatory effects on different types of respiratory diseases; however, it is yet to be determined whether it is effective in patients with idiopathic pulmonary fibrosis (IPF). The purpose of this study is to explore the capacity of QAFE to fight fibrotic disease, in particular how it works in relation to the regulation of the nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase 1 (HO-1) pathway. QAFE was made with Quzhou Fructus Aurantii (QAF), and the content of four flavonoids in the samples prepared was analyzed by high-performance liquid chromatography (HPLC). The therapeutic effect of QAFE was experimented by establishing IPF models in mice and in cells. Identification of the mechanism of QAFE in IPF through knockout or knockdown of the Nrf2 gene. The experiments suggest that QAFE has the potential to prevent IPF-induced inflammation, collagen deposition, oxidative stress, and apoptosis of cells. Silencing Nrf2 by knockdown or knockout is enough to prevent the capacity of QAFE to inhibit the process of inflammation, oxidative stress, and collagen deposition to cause more serious lung injury and HO-1 expression downregulation. QAFE is a potential new antifibrotic drug in IPF with an effect on the Nrf2/HO-1 pathway that reduces inflammation and oxidative stress. Full article

The Duolong porphyry copper–gold district, located in the northwestern segment of the Bangongco–Nujiang metallogenic belt on the southern margin of the South Qiangtang terrane (Tibet), hosts typical porphyry-style Cu-Au mineralization with well-defined alteration zoning from potassic through chlorite–sericite to propylitic assemblages. Based on integrated in situ major/trace element and sulfur isotope analyses of pyrite and chalcopyrite from different alteration zones, we identify two discrete episodes of magmatic-hydrothermal activity that exerted distinct controls on metal endowment. Sulfur isotope signatures define a systematic evolution from the earliest, high-temperature potassic stage (δ³⁴S: Py-Ⅰ –3.70 to –1.16‰, mean –2.14‰; Cp-Ⅰ –4.92 to –0.90‰, mean –2.54‰) through propylitic alteration (Py-Ⅱ: 1.20–5.16‰, mean 3.06‰) to the later chlorite–sericite stage (Py-Ⅲ: –2.00 to 1.86‰, mean 0.06‰; Cp-Ⅱ: –2.50 to 0.58‰, mean –0.77‰), tracking progressive fluid cooling and changing fluid sources. Trace element systematics further discriminate these episodes: sulfides from potassic and chlorite–sericite zones are enriched in trace elements, whereas propylitic pyrite is depleted, with potassic pyrite recording the highest Cu concentrations (559–7256 ppm, mean 2302 ppm) and chlorite–sericite pyrite containing the lowest Au contents (0.01–0.59 ppm, mean 0.10 ppm). Gold mineralization occurs as native gold exsolved from chalcopyrite, and the markedly low Au concentrations in chlorite–sericite pyrite (0.01–0.59 ppm, mean 0.10 ppm) demonstrate that gold exsolution was largely completed during the first, high-temperature magmatic-hydrothermal stage. Collectively, these results establish a detailed geochemical framework linking sulfide composition to specific hydrothermal stages, providing new constraints on the processes of porphyry copper–gold mineralization in a collisional setting. Full article

This study investigates the influence of physiological body fluids on the mass stability and tribological performance of polylactic acid (PLA) samples produced by Fused Deposition Modeling (FDM) 3D printing. Body fluid exposure was simulated using Dulbecco’s Modified Eagle Medium (DMEM) under controlled conditions. Black PLA filament was printed with three infill densities (15%, 20%, and 90%) and immersed in DMEM for 7 days at 37 ± 1 °C. Mass measurements revealed that lower infill densities resulted in significantly higher mass loss, with the 15% infill samples exhibiting the greatest reduction (5.07%), while the 90% infill samples showed negligible change (0.17%). Tribological testing using a CSM nanotribometer under loads of 5 mN, 500 mN, and 1000 mN demonstrated that infill density critically affects friction and wear behavior. The 90% infill samples exhibited the lowest wear volumes and the most stable tribological response, while the 15% infill samples showed degradation-dominated contact behavior. Although the friction measurements for the 15% infill samples were consistent, their interpretation should be approached with caution due to pronounced surface deterioration and debris-mediated sliding. This behavior is attributed to structural weakening caused by immersion in DMEM, which promoted material degradation and influenced the tribological response. These findings confirm the critical role of structural density in wear resistance. To the best of our knowledge, this is the first study to systematically investigate the combined effect of hydrolytic degradation and tribological behavior of FDM-printed PLA as a function of infill density under simulated physiological conditions. These findings provide a scientific basis for optimizing infill density in the design of PLA-based surgical instrument guides, where both degradation resistance and tribological performance under body fluid exposure are essential. The findings should be interpreted within the limitations of the experimental design. Full article

Hybrid robotic manufacturing systems integrating additive and subtractive processes enable fabrication of complex, high-value components but are typically executed sequentially, resulting in long cycle times. Concurrent execution of Directed Energy Deposition (DED) and milling promises productivity gains but introduces coupled thermal, mechanical and spatial interactions that challenge conventional process planning. This work addresses the methodological problem of planning milling operations in the presence of an ongoing DED process. The concurrent planning task is formulated as a mixed-integer, nonlinear, multi-objective optimisation problem capturing sequencing and orientation decisions, cutting parameters and enabling temporal coupling to the deposition trajectory. A hierarchical, surrogate-assisted optimisation framework is proposed, combining unified decision-variable encoding, deterministic decoding and staged feasibility enforcement to ensure robotic executability. Disturbance mechanisms such as thermal interaction, particulate interference and pose-dependent dynamic compatibility are incorporated as modular objective abstractions, enabling systematic trade-offs between machining productivity and preservation of deposition process integrity. The proposed framework is demonstrated on a representative case study, enabling analysis of the interaction between spatial sequencing, temporal feasibility and disturbance-aware optimisation. The case study provides a controlled instantiation and illustrates its application to concurrent additive–subtractive planning under explicitly modelled temporal and disturbance constraints. Full article

In sediment-laden irrigation channels, sediment deposition upstream of hydraulic measuring structures can degrade hydraulic performance, reduce measurement reliability, and increase maintenance demand. To clarify the effects of structural optimization on sediment transport and siltation resistance, physical model experiments were conducted on an airfoil weir-orifice facility under different discharges, structural angles, and sediment concentrations. The analysis focused on sediment deposition patterns, longitudinal water surface profiles, sediment concentration, suspended sediment transport rate, cross-sectional velocity distribution, vertical velocity gradient, and Froude number. The results showed that the optimized configuration produced a flatter and more uniform upstream bed morphology, and the average deposition thickness decreased from 4.83 cm to 4.31 cm, corresponding to a reduction of 10.58%. Under all tested conditions, the optimized configuration reduced upstream backwater, increased local flow velocity, and shifted the hydraulic jump closer to the facility outlet. Sediment concentration and suspended sediment transport rate were consistently higher after optimization, indicating enhanced sediment carrying capacity. In addition, the optimized configuration increased the vertical velocity gradient and Froude number, while all cases remained within the subcritical-flow regime. These findings demonstrate that structural optimization can simultaneously improve hydraulic regulation and siltation resistance, and provide an experimental basis for the application of streamlined hydraulic measuring structures in sediment-laden irrigation channels. Full article

To address the severe snow accumulation within road cuttings triggered by wind-blown snow on mountainous highways, and to elucidate the influence mechanisms of cutting depth and snow fence parameters on the wind–snow flow field, this study presents a systematic investigation based on typical sections of the G577 Grade I mountain highway in the Xinjiang Uygur Autonomous Region, China. First, indoor wind tunnel experiments were conducted to observe the distribution characteristics of the wind– snow field inside and outside the cuttings and around the snow fences under varying cutting depths and fence parameters. Second, numerical simulations were performed using the Analysis System Fluent software with models identical to those used in the wind tunnel tests to analyze the airflow field and snow particle movement patterns. Finally, experimental results were compared with field observations of winter snow accumulation to validate the reliability of both the numerical simulations and wind tunnel experiments. The results indicate that under small intersection angles (15–30°), deep cuttings significantly exacerbate snowdrift accumulation trends, reducing wind speed within the cutting and increasing snow accumulation at the bottom (an increase of 31–81% per 5 m of depth). Furthermore, a nonlinear relationship regarding the impact of different snow fence parameters on flow field distribution. These findings provide theoretical support and valuable engineering references for optimizing road cutting design and snow fence construction in mountainous regions. Full article

Concentrated Solar Power (CSP) tower systems require receiver materials capable of operating above 1000 °C to meet the efficiency targets of third-generation technologies (25–30%). Hybrid solutions, combining ceramic coatings with metallic substrates, offer promising thermomechanical stability under severe thermal cycling. This study investigates the high-temperature behavior of silicon carbide (SiC) coatings deposited on Fe-C-Al-Mo alloys under concentrated solar flux. Substrates were pre-oxidized to form a continuous 1–2 µm α-Al₂O₃ interlayer, serving as a chemical and mechanical buffer. SiC coatings (10–24 µm thick) were deposited via High-Temperature Chemical Vapor Deposition (HT-CVD). Characterization using XRD, SEM, EDS, and optical spectrophotometry identified cubic 3C-SiC with a globular microstructure and high compressive residual stresses (−2000 to −2400 MPa), inducing microcracking. Stress relaxation was achieved by increasing coating thickness or post-deposition annealing. Controlled oxidation formed a thin silica layer, enhancing solar absorptivity to over 90%. Accelerated thermal cycling (up to ~900 kW/m², 1050–1200 °C) revealed that coating stability depends on SiC thickness, residual stress evolution, α-Al₂O₃ interlayer thickness, and cycling severity. Optimizing these parameters is essential for ensuring the long-term durability of hybrid CSP receivers. Full article

Background: Idiopathic pulmonary fibrosis (IPF) is a progressive lung disease marked by excessive extracellular matrix (ECM) deposition. Current FDA-approved therapies, such as pirfenidone and nintedanib, offer limited efficacy in halting disease progression. Lysyl oxidase-like 2 (LOXL2) is a key enzyme involved in ECM remodeling and fibrosis. This study investigates Compound #765, a novel indolizine derivative, as a potential LOXL2 inhibitor for IPF treatment. Methods: Compound #765 was synthesized and characterized using spectroscopic methods. Its inhibitory effect on LOXL2 activity was evaluated using LOXL2 enzymatic assays, in vitro fibrosis models with human lung fibroblasts, and in vivo models of pulmonary fibrosis, including bleomycin-treated and TGF-β1-overexpressing transgenic mice. In silico docking studies predicted the binding affinity of Compound #765 to LOXL2. Results: Compound #765 targeted LOXL2 activity and reduced collagen production in lung fibroblasts. In both bleomycin-induced pulmonary fibrosis and TGF-β1-overexpressing murine models, Compound #765 significantly alleviated fibrosis, as indicated by reduced collagen accumulation and inflammatory cell infiltration. The in silico docking studies predicted favorable binding affinity to LOXL2, which was confirmed through in vitro experiments. Importantly, Compound #765 suppressed fibrosis-associated markers in fibroblasts derived from IPF patients, suggesting translational potential. Conclusions: These results demonstrate that Compound #765 functions as a LOXL2 inhibitor with significant anti-fibrotic effects in vitro and in vivo, offering a promising therapeutic approach for IPF and other fibrotic lung diseases. Full article

The identification of ore types that share similar geological characteristics and metallurgical performance in a deposit is of great relevance in mine planning. In the case of a low-grade iron ore from Brazil, called itabirite, ore types are usually classified as compact and friable, in addition to canga. As itabirites become more widely exploited, friable itabirites have become scarcer, leaving more competent ores to be processed. The work investigates the response of 19 iron ore samples from the Serra do Sapo deposit (Minas Gerais, Brazil), through a variety of bench-scale comminution tests. In the context of crushing (>25 mm), one subtype of compact itabirite, called supercompact, presented substantially higher resistance to fragmentation than those of compact itabirite and canga. In the context of grinding (<19 mm), an inversion occurs, with canga presenting the highest resistance to comminution, followed by the itabirites (friable, compact, and supercompact), nearly indistinctively. This demonstrates that the relative competence of iron ores to withstand comminution in the studied mineral deposits varies significantly as a function of particle size and, therefore, size reduction stage. Finally, grouping of the samples using cluster analysis demonstrated the relevance of discrimination between compact and supercompact itabirites, besides canga, with supercompact itabirite having a greater affinity to canga than with its compact counterpart. This shows the importance of further discriminating itabirites, particularly in the context of comminution at coarser sizes. Full article

Plasma-assisted ammonia (NH₃) decomposition is a promising strategy for hydrogen production. However, reactor geometry remains a key factor limiting its hydrogen yield per energy input (${\mathrm{Y}}_{{\mathrm{H}}_2}$). This study systematically investigates H₂ production in outer-dielectric (OD), inner-dielectric (ID), and double-dielectric (DD) coaxial DBD reactors. The results show that the ammonia decomposition performance of OD- and ID-coaxial DBDs is significantly higher than that of the DD-coaxial DBD. OD- and ID-coaxial DBDs generate abundant micro-discharge pulses, enabling effective discharge energy deposition at lower peak voltages. Consequently, the reduced electric fields E/N are maintained within the optimal kinetic window for NH₃ dissociation and H₂ production. Moreover, by balancing residence time and energy density, the 8 cm length electrode achieves a peak ${\mathrm{Y}}_{{\mathrm{H}}_2}$ of 1.22–1.24 gH₂/kWh in the OD-coaxial DBD. For the ID-coaxial DBD, a 1 mm dielectric thickness yields a maximum capacitance of 86 pF, achieving a peak ${\mathrm{Y}}_{{\mathrm{H}}_2}$ of ~1.35 gH₂/kWh at the optimum E/N. In contrast, the DD-coaxial DBD exhibits the lowest ${\mathrm{Y}}_{{\mathrm{H}}_2}$ (≤0.82 gH₂/kWh) with minimal temperature rise. This is caused by the reduced current pulse numbers and the deviation of E/N from the optimal range with elevated operating voltages. This work provides guidance for the optimization of DBD reactors in plasma-assisted NH₃ decomposition for efficient H₂ production. Full article

Sandstone-type uranium deposits represent one of the most significant uranium deposit types in China, predominantly hosted in Meso-Cenozoic sedimentary basins in the northern part of the country. Due to characteristics such as deep burial of orebodies, fine grain size of ores, and strong heterogeneity, traditional geological logging methods have limitations in rapidly and accurately identifying alteration minerals and mineralization indicator information. Core spectral technology (wavelength range approximately 400–2500 nm), particularly short-wave infrared spectroscopy (SWIR, 1300–2500 nm), enables rapid, non-destructive, and quantitative extraction of alteration mineral information from drill cores. This provides robust technical support for reconstructing metallogenic environments, delineating oxidation–reduction zones, and prospecting and prediction in sandstone-type uranium deposits. This review systematically examines the spectral absorption characteristics and geological significance of key alteration minerals (e.g., clay minerals, carbonate minerals, iron oxides, and hydrocarbon substances) in sandstone-type uranium deposits. It elaborates on the current application status of core spectral technology in sandstone-type uranium exploration within typical basins in northern China, such as the Ordos, Songliao, Erlian, and Qaidam Basins. These applications include alteration mineral mapping, oxidation–reduction zone delineation, and metallogenic fluid tracing. Due to the unique characteristics of host rock lithology, alteration mineral assemblages, and fluid properties in sandstone-type uranium deposits, the application of this technology also faces certain challenges, such as difficulties in spectral interpretation and insufficient accuracy in quantitative inversion. Integrating this technique with multiple methods, including petrography and X-ray diffraction (XRD), will facilitate more effective applications in both metallogenic research and prospecting practices for sandstone-type uranium deposits in northern China. Full article

The Linhe Formation of the Paleogene in the Linhua Well area of the Hetao Basin is a key target interval for hydrocarbon exploration, but strong heterogeneity caused by depositional and diagenetic modification complicates reservoir prediction. This study integrates core observations, thin-section petrography, SEM, clay mineral XRD, vitrinite reflectance (Ro), routine petrophysical data, and conventional well logs to characterize sedimentary microfacies and diagenesis, constrain the diagenetic stage and paragenetic sequence, establish a well-log-based diagenetic facies recognition model, and reveal the spatial distribution of diagenetic facies. The reservoirs are dominated by lithic arkoses and feldspathic litharenites with moderate compositional and textural maturity. Sedimentary microfacies mainly include a subaqueous distributary channel, front sheet sand, and interdistributary bay. The reservoirs are presently overall in mesodiagenetic stage A. Compaction and cementation are the principal destructive processes, whereas dissolution is the main constructive process. Quantitative evaluation shows that COPL ranges from 14.3% to 31.6% (average 25.2%), CEPL from 5.3% to 18.7% (average 12.7%), and ICOMPACT from 0.47 to 0.80 (average 0.66), indicating that compaction contributed more to porosity loss than cementation. Four diagenetic facies were identified: strongly compacted–weakly cemented, moderately compacted–strongly cemented, moderately dissolved–moderately cemented, and weakly compacted–weakly cemented. Fisher’s linear discriminant model based on GR, AC, DEN, and CNL logs achieved an overall recognition accuracy of 80.0%. Spatially, high-quality reservoirs are mainly developed in the central–southern subaqueous distributary channel belts dominated by the weakly compacted–weakly cemented facies and flanked by moderately dissolved–moderately cemented facies. High-quality reservoir development is controlled by the coupled effects of depositional microfacies, differential compaction–cementation, and local dissolution. Full article

Panzhihua-type V–Ti magnetite deposits in the Panxi region are hosted in mafic–ultramafic intrusions, and their exploration potential depends strongly on the deep distribution of ore-bearing intrusions. High-resolution 3D magnetic inversion is an effective tool to image the geometry of these intrusions. Using 1:50,000 aeromagnetic data, we applied an unsupervised deep learning inversion to obtain the 3D magnetic susceptibility structure of related intrusions. The results show that magnetic anomalies are mainly NS and NEE trending, with minor NNW-trending features. NS-trending sources occur in the Baima–Miyi–Hongge zone between the Xigeda–Yuanmou and Anninghe faults, while NEE-trending anomalies lie west of the Xigeda–Yuanmou fault and east of the Chenghai fault. Integrated geological analysis reveals two Late Variscan rift systems: the Anninghe rift and the Panzhihua rift. Deep fault-controlled magma ascent and emplacement, forming the Emeishan large igneous province, are associated with strongly magnetic intrusions. Mantle plume-derived magmas, differentiated in shallow and deep magma chambers, generate well-differentiated layered complexes at depths < 10 km with magnetic intensities of 5–10 A/m. Shear structures within paleorifts provide favorable emplacement conditions and controlled ore localization. We propose a three-in-one ore-controlling mechanism involving rift systems, intrusive rocks, and shear structures for Panzhihua-type V–Ti magnetite mineralization. Full article