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Keywords = deep learning for plant health

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34 pages, 3764 KiB  
Review
Research Progress and Applications of Artificial Intelligence in Agricultural Equipment
by Yong Zhu, Shida Zhang, Shengnan Tang and Qiang Gao
Agriculture 2025, 15(15), 1703; https://doi.org/10.3390/agriculture15151703 - 7 Aug 2025
Abstract
With the growth of the global population and the increasing scarcity of arable land, traditional agricultural production is confronted with multiple challenges, such as efficiency improvement, precision operation, and sustainable development. The progressive advancement of artificial intelligence (AI) technology has created a transformative [...] Read more.
With the growth of the global population and the increasing scarcity of arable land, traditional agricultural production is confronted with multiple challenges, such as efficiency improvement, precision operation, and sustainable development. The progressive advancement of artificial intelligence (AI) technology has created a transformative opportunity for the intelligent upgrade of agricultural equipment. This article systematically presents recent progress in computer vision, machine learning (ML), and intelligent sensing. The key innovations are highlighted in areas such as object detection and recognition (e.g., a K-nearest neighbor (KNN) achieved 98% accuracy in distinguishing vibration signals across operation stages); autonomous navigation and path planning (e.g., a deep reinforcement learning (DRL)-optimized task planner for multi-arm harvesting robots reduced execution time by 10.7%); state perception (e.g., a multilayer perceptron (MLP) yielded 96.9% accuracy in plug seedling health classification); and precision control (e.g., an intelligent multi-module coordinated control system achieved a transplanting efficiency of 5000 plants/h). The findings reveal a deep integration of AI models with multimodal perception technologies, significantly improving the operational efficiency, resource utilization, and environmental adaptability of agricultural equipment. This integration is catalyzing the transition toward intelligent, automated, and sustainable agricultural systems. Nevertheless, intelligent agricultural equipment still faces technical challenges regarding data sample acquisition, adaptation to complex field environments, and the coordination between algorithms and hardware. Looking ahead, the convergence of digital twin (DT) technology, edge computing, and big data-driven collaborative optimization is expected to become the core of next-generation intelligent agricultural systems. These technologies have the potential to overcome current limitations in perception and decision-making, ultimately enabling intelligent management and autonomous decision-making across the entire agricultural production chain. This article aims to provide a comprehensive foundation for advancing agricultural modernization and supporting green, sustainable development. Full article
(This article belongs to the Section Agricultural Technology)
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17 pages, 54671 KiB  
Article
Pep-VGGNet: A Novel Transfer Learning Method for Pepper Leaf Disease Diagnosis
by Süleyman Çetinkaya and Amira Tandirovic Gursel
Appl. Sci. 2025, 15(15), 8690; https://doi.org/10.3390/app15158690 - 6 Aug 2025
Abstract
The health of crops is a major challenge for productivity growth in agriculture, with plant diseases playing a key role in limiting crop yield. Identifying and understanding these diseases is crucial to preventing their spread. In particular, greenhouse pepper leaves are susceptible to [...] Read more.
The health of crops is a major challenge for productivity growth in agriculture, with plant diseases playing a key role in limiting crop yield. Identifying and understanding these diseases is crucial to preventing their spread. In particular, greenhouse pepper leaves are susceptible to diseases such as mildew, mites, caterpillars, aphids, and blight, which leave distinctive marks that can be used for disease classification. The study proposes a seven-class classifier for the rapid and accurate diagnosis of pepper diseases, with a primary focus on pre-processing techniques to enhance colour differentiation between green and yellow shades, thereby facilitating easier classification among the classes. A novel algorithm is introduced to improve image vibrancy, contrast, and colour properties. The diagnosis is performed using a modified VGG16Net model, which includes three additional layers for fine-tuning. After initialising on the ImageNet dataset, some layers are frozen to prevent redundant learning. The classification is additionally accelerated by introducing flattened, dense, and dropout layers. The proposed model is tested on a private dataset collected specifically for this study. Notably, this work is the first to focus on diagnosing aphid and caterpillar diseases in peppers. The model achieves an average accuracy of 92.00%, showing promising potential for seven-class deep learning-based disease diagnostics. Misclassifications in the aphid class are primarily due to the limited number of samples available. Full article
(This article belongs to the Section Computing and Artificial Intelligence)
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18 pages, 2644 KiB  
Article
Multispectral and Chlorophyll Fluorescence Imaging Fusion Using 2D-CNN and Transfer Learning for Cross-Cultivar Early Detection of Verticillium Wilt in Eggplants
by Dongfang Zhang, Shuangxia Luo, Jun Zhang, Mingxuan Li, Xiaofei Fan, Xueping Chen and Shuxing Shen
Agronomy 2025, 15(8), 1799; https://doi.org/10.3390/agronomy15081799 - 25 Jul 2025
Viewed by 173
Abstract
Verticillium wilt is characterized by chlorosis in leaves and is a devastating disease in eggplant. Early diagnosis, prior to the manifestation of symptoms, enables targeted management of the disease. In this study, we aim to detect early leaf wilt in eggplant leaves caused [...] Read more.
Verticillium wilt is characterized by chlorosis in leaves and is a devastating disease in eggplant. Early diagnosis, prior to the manifestation of symptoms, enables targeted management of the disease. In this study, we aim to detect early leaf wilt in eggplant leaves caused by Verticillium dahliae by integrating multispectral imaging with machine learning and deep learning techniques. Multispectral and chlorophyll fluorescence images were collected from leaves of the inbred eggplant line 11-435, including data on image texture, spectral reflectance, and chlorophyll fluorescence. Subsequently, we established a multispectral data model, fusion information model, and multispectral image–information fusion model. The multispectral image–information fusion model, integrated with a two-dimensional convolutional neural network (2D-CNN), demonstrated optimal performance in classifying early-stage Verticillium wilt infection, achieving a test accuracy of 99.37%. Additionally, transfer learning enabled us to diagnose early leaf wilt in another eggplant variety, the inbred line 14-345, with an accuracy of 84.54 ± 1.82%. Compared to traditional methods that rely on visible symptom observation and typically require about 10 days to confirm infection, this study achieved early detection of Verticillium wilt as soon as the third day post-inoculation. These findings underscore the potential of the fusion model as a valuable tool for the early detection of pre-symptomatic states in infected plants, thereby offering theoretical support for in-field detection of eggplant health. Full article
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20 pages, 41202 KiB  
Article
Copper Stress Levels Classification in Oilseed Rape Using Deep Residual Networks and Hyperspectral False-Color Images
by Yifei Peng, Jun Sun, Zhentao Cai, Lei Shi, Xiaohong Wu, Chunxia Dai and Yubin Xie
Horticulturae 2025, 11(7), 840; https://doi.org/10.3390/horticulturae11070840 - 16 Jul 2025
Viewed by 274
Abstract
In recent years, heavy metal contamination in agricultural products has become a growing concern in the field of food safety. Copper (Cu) stress in crops not only leads to significant reductions in both yield and quality but also poses potential health risks to [...] Read more.
In recent years, heavy metal contamination in agricultural products has become a growing concern in the field of food safety. Copper (Cu) stress in crops not only leads to significant reductions in both yield and quality but also poses potential health risks to humans. This study proposes an efficient and precise non-destructive detection method for Cu stress in oilseed rape, which is based on hyperspectral false-color image construction using principal component analysis (PCA). By comprehensively capturing the spectral representation of oilseed rape plants, both the one-dimensional (1D) spectral sequence and spatial image data were utilized for multi-class classification. The classification performance of models based on 1D spectral sequences was compared from two perspectives: first, between machine learning and deep learning methods (best accuracy: 93.49% vs. 96.69%); and second, between shallow and deep convolutional neural networks (CNNs) (best accuracy: 95.15% vs. 96.69%). For spatial image data, deep residual networks were employed to evaluate the effectiveness of visible-light and false-color images. The RegNet architecture was chosen for its flexible parameterization and proven effectiveness in extracting multi-scale features from hyperspectral false-color images. This flexibility enabled RegNetX-6.4GF to achieve optimal performance on the dataset constructed from three types of false-color images, with the model reaching a Macro-Precision, Macro-Recall, Macro-F1, and Accuracy of 98.17%, 98.15%, 98.15%, and 98.15%, respectively. Furthermore, Grad-CAM visualizations revealed that latent physiological changes in plants under heavy metal stress guided feature learning within CNNs, and demonstrated the effectiveness of false-color image construction in extracting discriminative features. Overall, the proposed technique can be integrated into portable hyperspectral imaging devices, enabling real-time and non-destructive detection of heavy metal stress in modern agricultural practices. Full article
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21 pages, 4147 KiB  
Article
AgriFusionNet: A Lightweight Deep Learning Model for Multisource Plant Disease Diagnosis
by Saleh Albahli
Agriculture 2025, 15(14), 1523; https://doi.org/10.3390/agriculture15141523 - 15 Jul 2025
Viewed by 495
Abstract
Timely and accurate identification of plant diseases is critical to mitigating crop losses and enhancing yield in precision agriculture. This paper proposes AgriFusionNet, a lightweight and efficient deep learning model designed to diagnose plant diseases using multimodal data sources. The framework integrates RGB [...] Read more.
Timely and accurate identification of plant diseases is critical to mitigating crop losses and enhancing yield in precision agriculture. This paper proposes AgriFusionNet, a lightweight and efficient deep learning model designed to diagnose plant diseases using multimodal data sources. The framework integrates RGB and multispectral drone imagery with IoT-based environmental sensor data (e.g., temperature, humidity, soil moisture), recorded over six months across multiple agricultural zones. Built on the EfficientNetV2-B4 backbone, AgriFusionNet incorporates Fused-MBConv blocks and Swish activation to improve gradient flow, capture fine-grained disease patterns, and reduce inference latency. The model was evaluated using a comprehensive dataset composed of real-world and benchmarked samples, showing superior performance with 94.3% classification accuracy, 28.5 ms inference time, and a 30% reduction in model parameters compared to state-of-the-art models such as Vision Transformers and InceptionV4. Extensive comparisons with both traditional machine learning and advanced deep learning methods underscore its robustness, generalization, and suitability for deployment on edge devices. Ablation studies and confusion matrix analyses further confirm its diagnostic precision, even in visually ambiguous cases. The proposed framework offers a scalable, practical solution for real-time crop health monitoring, contributing toward smart and sustainable agricultural ecosystems. Full article
(This article belongs to the Special Issue Computational, AI and IT Solutions Helping Agriculture)
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12 pages, 2844 KiB  
Article
End-to-End Deep Learning Approach to Automated Phenotyping of Greenhouse-Grown Plant Shoots
by Evgeny Gladilin, Narendra Narisetti, Kerstin Neumann and Thomas Altmann
Agronomy 2025, 15(5), 1117; https://doi.org/10.3390/agronomy15051117 - 30 Apr 2025
Viewed by 406
Abstract
High-throughput image analysis is a key tool for the efficient assessment of quantitative plant phenotypes. A typical approach to the computation of quantitative plant traits from image data consists of two major steps including (i) image segmentation followed by (ii) calculation of quantitative [...] Read more.
High-throughput image analysis is a key tool for the efficient assessment of quantitative plant phenotypes. A typical approach to the computation of quantitative plant traits from image data consists of two major steps including (i) image segmentation followed by (ii) calculation of quantitative traits of segmented plant structures. Despite substantial advancements in deep learning-based segmentation techniques, minor artifacts of image segmentation cannot be completely avoided. For several commonly used traits including plant width, height, convex hull, etc., even small inaccuracies in image segmentation can lead to large errors. Ad hoc approaches to cleaning ’small noisy structures’ are, in general, data-dependent and may lead to substantial loss of relevant small plant structures and, consequently, falsified phenotypic traits. Here, we present a straightforward end-to-end approach to direct computation of phenotypic traits from image data using a deep learning regression model. Our experimental results show that image-to-trait regression models outperform a conventional segmentation-based approach for a number of commonly sought plant traits of plant morphology and health including shoot area, linear dimensions and color fingerprints. Since segmentation is missing in predictions of regression models, visualization of activation layer maps can still be used as a blueprint to model explainability. Although end-to-end models have a number of limitations compared to more complex network architectures, they can still be of interest for multiple phenotyping scenarios with fixed optical setups (such as high-throughput greenhouse screenings), where the accuracy of routine trait predictions and not necessarily the generalizability is the primary goal. Full article
(This article belongs to the Special Issue Novel Approaches to Phenotyping in Plant Research)
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27 pages, 7107 KiB  
Article
CBACA-YOLOv5: A Symmetric and Asymmetric Attention-Driven Detection Framework for Citrus Leaf Disease Identification
by Jiaxian Zhu, Jiahong Chen, Huiyang He, Weihua Bai and Teng Zhou
Symmetry 2025, 17(4), 617; https://doi.org/10.3390/sym17040617 - 18 Apr 2025
Viewed by 522
Abstract
The citrus industry plays a pivotal role in modern agriculture. With the expansion of citrus plantations, the intelligent detection and prevention of diseases and pests have become essential for advancing smart agriculture. Traditional citrus leaf disease identification methods primarily rely on manual observation, [...] Read more.
The citrus industry plays a pivotal role in modern agriculture. With the expansion of citrus plantations, the intelligent detection and prevention of diseases and pests have become essential for advancing smart agriculture. Traditional citrus leaf disease identification methods primarily rely on manual observation, which is often time-consuming, labor-intensive, and prone to inaccuracies due to inherent asymmetries in disease manifestations. This work introduces CBACA-YOLOv5, an enhanced YOLOv5s-based detection algorithm designed to effectively capture the symmetric and asymmetric features of common citrus leaf diseases. Specifically, the model integrates the convolutional block attention module (CBAM), which symmetrically enhances feature extraction across spatial and channel dimensions, significantly improving the detection of small and occluded targets. Additionally, we incorporate coordinate attention (CA) mechanisms into the YOLOv5s C3 module, explicitly addressing asymmetrical spatial distributions of disease features. The CARAFE upsampling module further optimizes feature fusion symmetry, enhancing the extraction efficiency and accelerating the network convergence. Experimental findings demonstrate that CBACA-YOLOv5 achieves an accuracy of 96.1% and a mean average precision (mAP) of 92.1%, and improvements of 0.6% and 2.3%, respectively, over the baseline model. The proposed CBACA-YOLOv5 model exhibits considerable robustness and reliability in detecting citrus leaf diseases under diverse and asymmetrical field conditions, thus holding substantial promise for practical integration into intelligent agricultural systems. Full article
(This article belongs to the Section Computer)
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46 pages, 1630 KiB  
Review
Optimization of Vegetable Production in Hydroculture Environments Using Artificial Intelligence: A Literature Review
by Dick Diaz-Delgado, Ciro Rodriguez, Augusto Bernuy-Alva, Carlos Navarro and Alexander Inga-Alva
Sustainability 2025, 17(7), 3103; https://doi.org/10.3390/su17073103 - 31 Mar 2025
Cited by 2 | Viewed by 2367
Abstract
This review analyzes the role of artificial intelligence (AI) and automation in optimizing vegetable production within hydroculture systems. Methods: Following the PRISMA methodology, this study examines research on IoT-based monitoring and AI techniques, particularly Deep Neural Networks (DNNs), K-Nearest Neighbors (KNNs), Fuzzy Logic [...] Read more.
This review analyzes the role of artificial intelligence (AI) and automation in optimizing vegetable production within hydroculture systems. Methods: Following the PRISMA methodology, this study examines research on IoT-based monitoring and AI techniques, particularly Deep Neural Networks (DNNs), K-Nearest Neighbors (KNNs), Fuzzy Logic (FL), Convolutional Neural Networks (CNNs), and Decision Trees (DTs). Additionally, Recurrent Neural Networks (RNNs) and Long Short-Term Memory (LSTM) models were analyzed due to their effectiveness in processing temporal data and improving predictive capabilities in nutrient optimization. These models have demonstrated high precision in managing key parameters such as pH, temperature, electrical conductivity, and nutrient dosing to enhance crop growth. The selection criteria focused on peer-reviewed studies from 2020 to 2024, emphasizing automation, efficiency, sustainability, and real-time monitoring. After filtering out duplicates and non-relevant papers, 72 studies from the IEEE, SCOPUS, MDPI, and Google Scholar databases were analyzed, focusing on the applicability of AI in optimizing vegetable production. Results: Among the AI models evaluated, Deep Neural Networks (DNNs) achieved 97.5% accuracy in crop growth predictions, while Fuzzy Logic (FL) demonstrated a 3% error rate in nutrient solution adjustments, ensuring reliable real-time decision-making. CNNs were the most effective for disease and pest detection, reaching a precision rate of 99.02%, contributing to reduced pesticide use and improved plant health. Random Forest (RF) and Support Vector Machines (SVMs) demonstrated up to 97.5% accuracy in optimizing water consumption and irrigation efficiency, promoting sustainable resource management. Additionally, LSTM and RNN models improved long-term predictions for nutrient absorption, optimizing hydroponic system control. Hybrid AI models integrating machine learning and deep learning techniques showed promise for enhancing system automation. Conclusion: AI-driven optimization in hydroculture improves nutrient management, water efficiency, and plant health monitoring, leading to higher yields and sustainability. Despite its benefits, challenges such as data availability, model standardization, and implementation costs persist. Future research should focus on enhancing model accessibility, interoperability, and real-world validation to expand AI adoption in smart agriculture. Furthermore, the integration of LSTM and RNN should be further explored to enhance real-time adaptability and improve the resilience of predictive models in hydroponic environments. Full article
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19 pages, 3485 KiB  
Article
Predicting the Distribution of Ailanthus altissima Using Deep Learning-Based Analysis of Satellite Imagery
by Ruohan Gao, Zipeng Song, Junhan Zhao and Yingnan Li
Symmetry 2025, 17(3), 324; https://doi.org/10.3390/sym17030324 - 21 Feb 2025
Viewed by 812
Abstract
Invasive species negatively affect ecosystems, economies, and human health by outcompeting native species and altering habitats. Ailanthus altissima, also known as the tree of heaven, an invasive species native to China that has spread to North America and Europe. Commonly found in [...] Read more.
Invasive species negatively affect ecosystems, economies, and human health by outcompeting native species and altering habitats. Ailanthus altissima, also known as the tree of heaven, an invasive species native to China that has spread to North America and Europe. Commonly found in urban areas and forestland, these invasive plants cause ecological and economic damage to local ecosystems; they are also the preferred host of other invasive species. Ecological stability refers to the balance and harmony in species populations. Invasive species like A. altissima disrupt this stability by outcompeting native species, leading to imbalances, and there was a lack of research and data on the tree of heaven. To address this issue, this study leveraged deep learning and satellite imagery recognition to generate reliable and comprehensive prediction maps in the USA. Four deep learning models were trained to recognize satellite images obtained from Google Earth, with A. altissima data obtained from the Life Alta Murgia project, LIFE12 BIO/IT/000213. The best performing fine-tuned model using binary classification achieved an AUC score of 90%. This model was saved locally and used to predict the density and probability of A. altissima in the USA. Additionally, multi-class classification methods corroborated the findings, demonstrating similar observational outcomes. The production of these predictive distribution maps is a novel method which offers an innovative and cost-effective alternative for extensive field surveys, providing reliable data for concurrent and future research on the environmental impact of A. altissima. Full article
(This article belongs to the Special Issue Applications of Symmetry in Computational Biology)
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20 pages, 7839 KiB  
Article
Normalized Difference Vegetation Index Prediction for Blueberry Plant Health from RGB Images: A Clustering and Deep Learning Approach
by A. G. M. Zaman, Kallol Roy and Jüri Olt
AgriEngineering 2024, 6(4), 4831-4850; https://doi.org/10.3390/agriengineering6040276 - 16 Dec 2024
Viewed by 1552
Abstract
In precision agriculture (PA), monitoring individual plant health is crucial for optimizing yields and minimizing resources. The normalized difference vegetation index (NDVI), a widely used health indicator, typically relies on expensive multispectral cameras. This study introduces a method for predicting the NDVI of [...] Read more.
In precision agriculture (PA), monitoring individual plant health is crucial for optimizing yields and minimizing resources. The normalized difference vegetation index (NDVI), a widely used health indicator, typically relies on expensive multispectral cameras. This study introduces a method for predicting the NDVI of blueberry plants using RGB images and deep learning, offering a cost-effective alternative. To identify individual plant bushes, K-means and Gaussian Mixture Model (GMM) clustering were applied. RGB images were transformed into the HSL (hue, saturation, lightness) color space, and the hue channel was constrained using percentiles to exclude extreme values while preserving relevant plant hues. Further refinement was achieved through adaptive pixel-to-pixel distance filtering combined with the Davies–Bouldin Index (DBI) to eliminate pixels deviating from the compact cluster structure. This enhanced clustering accuracy and enabled precise NDVI calculations. A convolutional neural network (CNN) was trained and tested to predict NDVI-based health indices. The model achieved strong performance with mean squared losses of 0.0074, 0.0044, and 0.0021 for training, validation, and test datasets, respectively. The test dataset also yielded a mean absolute error of 0.0369 and a mean percentage error of 4.5851. These results demonstrate the NDVI prediction method’s potential for cost-effective, real-time plant health assessment, particularly in agrobotics. Full article
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23 pages, 1660 KiB  
Article
A Deep Learning Model for Accurate Maize Disease Detection Based on State-Space Attention and Feature Fusion
by Tong Zhu, Fengyi Yan, Xinyang Lv, Hanyi Zhao, Zihang Wang, Keqin Dong, Zhengjie Fu, Ruihao Jia and Chunli Lv
Plants 2024, 13(22), 3151; https://doi.org/10.3390/plants13223151 - 9 Nov 2024
Cited by 4 | Viewed by 2313
Abstract
In improving agricultural yields and ensuring food security, precise detection of maize leaf diseases is of great importance. Traditional disease detection methods show limited performance in complex environments, making it challenging to meet the demands for precise detection in modern agriculture. This paper [...] Read more.
In improving agricultural yields and ensuring food security, precise detection of maize leaf diseases is of great importance. Traditional disease detection methods show limited performance in complex environments, making it challenging to meet the demands for precise detection in modern agriculture. This paper proposes a maize leaf disease detection model based on a state-space attention mechanism, aiming to effectively utilize the spatiotemporal characteristics of maize leaf diseases to achieve efficient and accurate detection. The model introduces a state-space attention mechanism combined with a multi-scale feature fusion module to capture the spatial distribution and dynamic development of maize diseases. In experimental comparisons, the proposed model demonstrates superior performance in the task of maize disease detection, achieving a precision, recall, accuracy, and F1 score of 0.94. Compared with baseline models such as AlexNet, GoogLeNet, ResNet, EfficientNet, and ViT, the proposed method achieves a precision of 0.95, with the other metrics also reaching 0.94, showing significant improvement. Additionally, ablation experiments verify the impact of different attention mechanisms and loss functions on model performance. The standard self-attention model achieved a precision, recall, accuracy, and F1 score of 0.74, 0.70, 0.72, and 0.72, respectively. The Convolutional Block Attention Module (CBAM) showed a precision of 0.87, recall of 0.83, accuracy of 0.85, and F1 score of 0.85, while the state-space attention module achieved a precision of 0.95, with the other metrics also at 0.94. In terms of loss functions, cross-entropy loss showed a precision, recall, accuracy, and F1 score of 0.69, 0.65, 0.67, and 0.67, respectively. Focal loss showed a precision of 0.83, recall of 0.80, accuracy of 0.81, and F1 score of 0.81. State-space loss demonstrated the best performance in these experiments, achieving a precision of 0.95, with recall, accuracy, and F1 score all at 0.94. These results indicate that the model based on the state-space attention mechanism achieves higher detection accuracy and better generalization ability in the task of maize leaf disease detection, effectively improving the accuracy and efficiency of disease recognition and providing strong technical support for the early diagnosis and management of maize diseases. Future work will focus on further optimizing the model’s spatiotemporal feature modeling capabilities and exploring multi-modal data fusion to enhance the model’s application in real agricultural scenarios. Full article
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20 pages, 2907 KiB  
Article
Detection of Aspergillus flavus in Figs by Means of Hyperspectral Images and Deep Learning Algorithms
by Cristian Cruz-Carrasco, Josefa Díaz-Álvarez, Francisco Chávez de la O, Abel Sánchez-Venegas and Juan Villegas Cortez
AgriEngineering 2024, 6(4), 3969-3988; https://doi.org/10.3390/agriengineering6040225 - 28 Oct 2024
Cited by 3 | Viewed by 1778
Abstract
Plant diseases cause economic losses and health risks, such as aflatoxins linked to liver cancer. These toxins, produced by fungi like Aspergillus flavus in figs, are often detected late through invasive methods or visual inspection. Since Spain, particularly Extremadura, is a key fig [...] Read more.
Plant diseases cause economic losses and health risks, such as aflatoxins linked to liver cancer. These toxins, produced by fungi like Aspergillus flavus in figs, are often detected late through invasive methods or visual inspection. Since Spain, particularly Extremadura, is a key fig producer, alternative detection methods are essential to preventing aflatoxins in the food chain. The aim of this research is the early detection of Aspergillus flavus fungus using non-invasive techniques with hyperspectral imaging and applying artificial intelligence techniques, in particular deep learning. The images were taken after inoculation of the microtoxin using 3 different concentrations, related to three different classes and healthy figs (healthy controls). The analysis of the hyperspectral images was performed at the pixel level. Firstly, a fully connected neural network was used to analyze the spectral signature associated with each pixel; secondly, the wavelet transform was applied to each spectral signature. The resulting images were fed to a convolutional neural network. The hyperparameters of the proposed models were adjusted based on the parameter tuning process that was performed. The results are promising, with 83% accuracy, 82.75% recall, and 83.25% F1-measure for the fully connected neural network. The high F1-measure demonstrates that the model’s performance is good. The model has a low incidence of false positives for samples that contain aflatoxin, while a higher number of false positives appears in healthy controls. Due to the presence of false negatives, this class also has a high recall. The convolutional neural network results, accuracy, recall, and F1 are 77.25%, indicating moderate model performance. Only class 3, with higher aflatoxin concentration, achieves high precision and low false positive incidence. Healthy controls exhibit a high presence of false negatives. In conclusion, we demonstrate the effectiveness of pixel-level analysis in identifying the presence of the fungus and the viability of the non-invasive techniques applied in improving food safety. Although further research is needed, in this study, the fully connected neural network model shows good performance with lower energy consumption. Full article
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23 pages, 641 KiB  
Review
Artificial Intelligence Techniques in Crop Yield Estimation Based on Sentinel-2 Data: A Comprehensive Survey
by Muhammet Fatih Aslan, Kadir Sabanci and Busra Aslan
Sustainability 2024, 16(18), 8277; https://doi.org/10.3390/su16188277 - 23 Sep 2024
Cited by 22 | Viewed by 15167
Abstract
This review explores the integration of Artificial Intelligence (AI) with Sentinel-2 satellite data in the context of precision agriculture, specifically for crop yield estimation. The rapid advancements in remote sensing technology, particularly through Sentinel-2’s high-resolution multispectral imagery, have transformed agricultural monitoring by providing [...] Read more.
This review explores the integration of Artificial Intelligence (AI) with Sentinel-2 satellite data in the context of precision agriculture, specifically for crop yield estimation. The rapid advancements in remote sensing technology, particularly through Sentinel-2’s high-resolution multispectral imagery, have transformed agricultural monitoring by providing critical data on plant health, soil moisture, and growth patterns. By leveraging Vegetation Indices (VIs) derived from these images, AI algorithms, including Machine Learning (ML) and Deep Learning (DL) models, can now predict crop yields with high accuracy. This paper reviews studies from the past five years that utilize Sentinel-2 and AI techniques to estimate yields for crops like wheat, maize, rice, and others. Various AI approaches are discussed, including Random Forests, Support Vector Machines (SVM), Convolutional Neural Networks (CNNs), and ensemble methods, all contributing to refined yield forecasts. The review identifies a notable gap in the standardization of methodologies, with researchers using different VIs and AI techniques for similar crops, leading to varied results. As such, this study emphasizes the need for comprehensive comparisons and more consistent methodologies in future research. The work underscores the significant role of Sentinel-2 and AI in advancing precision agriculture, offering valuable insights for future studies that aim to enhance sustainability and efficiency in crop management through advanced predictive models. Full article
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25 pages, 3397 KiB  
Article
Hyperspectral Imaging for Phenotyping Plant Drought Stress and Nitrogen Interactions Using Multivariate Modeling and Machine Learning Techniques in Wheat
by Frank Gyan Okyere, Daniel Kingsley Cudjoe, Nicolas Virlet, March Castle, Andrew Bernard Riche, Latifa Greche, Fady Mohareb, Daniel Simms, Manal Mhada and Malcolm John Hawkesford
Remote Sens. 2024, 16(18), 3446; https://doi.org/10.3390/rs16183446 - 17 Sep 2024
Cited by 9 | Viewed by 4704
Abstract
Accurate detection of drought stress in plants is essential for water use efficiency and agricultural output. Hyperspectral imaging (HSI) provides a non-invasive method in plant phenotyping, allowing the long-term monitoring of plant health due to sensitivity to subtle changes in leaf constituents. The [...] Read more.
Accurate detection of drought stress in plants is essential for water use efficiency and agricultural output. Hyperspectral imaging (HSI) provides a non-invasive method in plant phenotyping, allowing the long-term monitoring of plant health due to sensitivity to subtle changes in leaf constituents. The broad spectral range of HSI enables the development of different vegetation indices (VIs) to analyze plant trait responses to multiple stresses, such as the combination of nutrient and drought stresses. However, known VIs may underperform when subjected to multiple stresses. This study presents new VIs in tandem with machine learning models to identify drought stress in wheat plants under varying nitrogen (N) levels. A pot wheat experiment was set up in the glasshouse with four treatments: well-watered high-N (WWHN), well-watered low-N (WWLN), drought-stress high-N (DSHN) and drought-stress low-N (DSLN). In addition to ensuring that plants were watered according to the experiment design, photosynthetic rate (Pn) and stomatal conductance (gs) (which are used to assess plant drought stress) were taken regularly, serving as the ground truth data for this study. The proposed VIs, together with known VIs, were used to train three classification models: support vector machines (SVM), random forest (RF), and deep neural networks (DNN) to classify plants based on their drought status. The proposed VIs achieved more than 0.94 accuracy across all models, and their performance further increased when combined with known VIs. The combined VIs were used to train three regression models to predict the stomatal conductance and photosynthetic rates of plants. The random forest regression model performed best, suggesting that it could be used as a stand-alone tool to forecast gs and Pn and track drought stress in wheat. This study shows that combining hyperspectral data with machine learning can effectively monitor and predict drought stress in crops, especially in varying nitrogen conditions. Full article
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17 pages, 4494 KiB  
Article
Sága, a Deep Learning Spectral Analysis Tool for Fungal Detection in Grains—A Case Study to Detect Fusarium in Winter Wheat
by Xinxin Wang, Gerrit Polder, Marlous Focker and Cheng Liu
Toxins 2024, 16(8), 354; https://doi.org/10.3390/toxins16080354 - 13 Aug 2024
Cited by 3 | Viewed by 1970
Abstract
Fusarium head blight (FHB) is a plant disease caused by various species of the Fusarium fungus. One of the major concerns associated with Fusarium spp. is their ability to produce mycotoxins. Mycotoxin contamination in small grain cereals is a risk to human and [...] Read more.
Fusarium head blight (FHB) is a plant disease caused by various species of the Fusarium fungus. One of the major concerns associated with Fusarium spp. is their ability to produce mycotoxins. Mycotoxin contamination in small grain cereals is a risk to human and animal health and leads to major economic losses. A reliable site-specific precise Fusarium spp. infection early warning model is, therefore, needed to ensure food and feed safety by the early detection of contamination hotspots, enabling effective and efficient fungicide applications, and providing FHB prevention management advice. Such precision farming techniques contribute to environmentally friendly production and sustainable agriculture. This study developed a predictive model, Sága, for on-site FHB detection in wheat using imaging spectroscopy and deep learning. Data were collected from an experimental field in 2021 including (1) an experimental field inoculated with Fusarium spp. (52.5 m × 3 m) and (2) a control field (52.5 m × 3 m) not inoculated with Fusarium spp. and sprayed with fungicides. Imaging spectroscopy data (hyperspectral images) were collected from both the experimental and control fields with the ground truth of Fusarium-infected ear and healthy ear, respectively. Deep learning approaches (pretrained YOLOv5 and DeepMAC on Global Wheat Head Detection (GWHD) dataset) were used to segment wheat ears and XGBoost was used to analyze the hyperspectral information related to the wheat ears and make predictions of Fusarium-infected wheat ear and healthy wheat ear. The results showed that deep learning methods can automatically detect and segment the ears of wheat by applying pretrained models. The predictive model can accurately detect infected areas in a wheat field, achieving mean accuracy and F1 scores exceeding 89%. The proposed model, Sága, could facilitate the early detection of Fusarium spp. to increase the fungicide use efficiency and limit mycotoxin contamination. Full article
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