Search Results (2,190)

The rapid growth of personal protective equipment (PPE) consumption has generated unprecedented volumes of polymer-based waste, posing a major challenge to the transition from a linear to a circular economic model. The challenges associated with PPE recycling are strongly linked to the sector of origin—including healthcare, laboratories, cleanrooms, and food processing—as this factor determines contamination levels and critically influences subsequent recycling steps. PPE waste originating from the healthcare sector requires stringent decontamination processes, which directly affect the final properties of recycled materials and their suitability for upcycling or downcycling applications. Another decisive factor is source segregation, together with labeling and sorting, given the intrinsic material heterogeneity of PPE, which commonly includes polypropylene (PP) masks, polycarbonate (PC) protective eyewear, and nitrile butadiene rubber (NBR) gloves. Mechanical and chemical recycling routes, including processes specifically developed for elastomeric materials, play a complementary role depending on the cleanliness and composition of the waste streams. The potential for downcycling and upcycling of recycled PPE is closely linked to polymer integrity and process compatibility. When appropriate segregation strategies and tailored recycling technologies are implemented, PPE waste can be effectively diverted from incineration. Under these conditions, PPE—once emblematic of single-use culture—can become a representative example of how complex polymer products may be reintegrated into sustainable material loops, contributing to resource efficiency and circular-economy objectives. Full article

Weight gain has been associated with integrase strand transfer inhibitors (INSTIs) in real-world studies; however, the causality of this relationship is unclear. Thus, we examined the effects of the INSTI, Dolutegravir (DTG), on human adipose cells in vitro and the reversibility of these effects by switching to a protease inhibitor, Darunavir (DRV). We established cultures of human adipose stem cells (ASCs) and newly differentiated adipocytes from individuals without HIV. For adipocytes, cells were exposed to DTG or DRV for 7 days, after which cells were maintained or switched to another ART. Experiments examining ASCs and the effects on adipogenesis initiated exposure during proliferation and continued throughout differentiation. Adipogenic outcomes included triglyceride content, gene expression, and adipokine secretion. Metabolic outcomes included lactate production, lipolysis, and oxygen consumption rates. DTG suppressed the secretion of adiponectin and leptin, and this was reversed following the switch to DRV in adipocytes without the altered expression of adipogenic genes. DTG exposure increased markers of endoplasmic reticulum stress, elevated lactate production, and suppressed oxygen consumption in ASCs. Exposure to DTG during differentiation lowered triglyceride accumulation and adiponectin secretion without altering the expression of adipogenic markers. Thus, DTG exposure resulted in changes in adipocyte function consistent with the progression of metabolically adverse phenotypes, and these effects were reversible. Full article

In the face of escalating environmental crises, sustainable development is becoming one of the key global challenges. A growing body of research indicates that transitioning to plant-based diets, including a vegan diet, can significantly improve environmental sustainability. Young people from Generation Z play a crucial role in shaping future consumption patterns, and their beliefs and attitudes toward sustainable food may serve as an important indicator of social changes in the area of nutrition and the potential for implementing pro-ecological practices. The aim of the study is to assess students’ perceptual attitudes toward plant-based products, which constitute an integral part of vegan diets, addressing a significant gap in the literature on sustainable consumption among Generation Z in emerging economies. The study allows for the identification of the main sources of information, psychosocial barriers, and motivational factors underlying the adoption of a vegan diet, situating them within sustainable development paradigms. The research group consisted of 263 university students in Warsaw (Poland). A CAWI survey method was used, with a proprietary questionnaire. The study shows that more than 60% of respondents were not interested in this dietary option, and a similar proportion did not expect to adopt veganism in the future. Students’ attitudes toward the vegan diet are shaped primarily by their self-assessment of nutritional knowledge, previous experiences with plant-based diets, and socio-cultural factors. Although many respondents recognize the health and environmental benefits of a plant-based diet, the lack of reliable knowledge, concerns about deficiencies, higher product costs, and social pressure remain key barriers limiting their willingness to change eating habits. From a practical perspective, the findings highlight the need to develop nutrition education focused on the competencies required to properly balance plant-based diets, to increase the economic and sensory accessibility of vegan food, and to counteract persistent stereotypes and misconceptions about veganism, especially on social media, which is the main source of information for this age group. Full article

The widespread use of antibiotics has led to the persistence of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in drinking water systems, posing potential public health risks at the point of use. In this study, a residential secondary water supply system (SWSS) in eastern China was investigated over one year to characterize microbial communities, ARB and ARG occurrence, and their associations with water quality in bulk water and biofilms. Culture-based methods, flow cytometry, quantitative PCR, and high-throughput 16S rRNA and ITS sequencing were applied. Although conventional treatment removed 94.8% of total bacteria, significant microbial regrowth occurred during secondary distribution, with the highest heterotrophic plate counts observed in rooftop storage tanks (up to 4718 CFU/mL). ARG concentrations increased along the distribution line, and the class 1 integron intI1 was enriched in downstream locations, indicating enhanced horizontal gene transfer potential. Sulfonamide resistance genes dominated the resistome, accounting for more than 60% of total ARG abundance in water samples. Seasonally, ARG levels were higher in autumn and winter, coinciding with elevated disinfectant residuals and lower temperatures. Chlorine was negatively associated with total bacterial abundance, while positive correlations were observed with the relative abundance of several ARGs when normalized to bacterial biomass, suggesting selective pressure under oxidative stress. Turbidity and bacterial abundance were positively correlated with ARB, particularly sulfonamide-resistant bacteria. Biofilms exhibited more stable microbial communities and provided microhabitats that facilitated microbial persistence. Notably, fungal abundance showed strong positive correlations with multiple ARGs, implying that microbial interactions may indirectly contribute to ARG persistence in SWSSs. These findings highlight the role of secondary distribution conditions, disinfectant pressure, and microbial interactions in shaping resistance risks in residential water supply systems, and provide insights for improving microbial risk management at the point of consumption. Full article

Interactive science galleries have transformed how the public engages with science, shifting from object-centred displays to immersive, design-led experiences. This study situates these changes within broader cultural and economic contexts, exploring how design mediates our understanding of science and reflects neoliberal and experiential values. Using archival research, qualitative interviews with museum professionals, and reflective practice, the research examines the evolution of interactive science spaces at the Science Museum in London—The Children’s Gallery, Launch Pad, and Wonderlab. The findings reveal that exhibition design increasingly prioritises entertainment, immersion, and pleasure, aligning with the rise in the experience economy and the influence of corporate models such as Disneyland. While such strategies enhance visitor engagement and accessibility, they risk simplifying complex scientific narratives and reducing learning to consumption. The study concludes that effective science communication design should balance enjoyment with critical inquiry, using both comfort and discomfort to foster curiosity, reflection, and ethical awareness. By analysing design’s role in shaping the “science experience”, this research contributes to understanding how cultural institutions can create more nuanced, thought-provoking encounters between audiences, knowledge, and space. Full article

Pathogens that have developed resistance to antibiotics pose a threat to public health. The primary goal in preventing foodborne infections is to inhibit the growth of and, subsequently, eliminate antibiotic-resistant pathogens at every stage from production to consumption. Escherichia coli, which has acquired resistance to most known antibiotics, is frequently found in chicken meat. In many countries, due to unregulated antibiotic use in poultry farming, poor hygiene in slaughterhouses, or cross-contamination, extended-spectrum beta-lactamase (ESBL)-producing E. coli has been identified as the causative agent in poultry-associated food poisoning. The need for more effective antimicrobial agents against this pathogen, which is resistant to existing antibiotics, has led to increased attention being paid to postbiotics produced by lactic acid bacteria, particularly bacteriocins. This study aimed to determine the antimicrobial effects of postbiotics obtained from kefir-derived Lactiplantibacillus plantarum and Lactococcus lactis against ESBL-positive E. coli. To achieve this, E. coli strains were isolated from raw chicken meat samples collected from the market using culture-based methods, and their antimicrobial resistance profiles were determined using the disk diffusion method. The ESBL positivity of the isolates was assessed using the double-disk synergy test. The antimicrobial activities of the postbiotics against the identified ESBL-positive E. coli strains were tested using the macro-dilution method to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values. ESBL-positive E. coli was detected in 48% of raw chicken meat samples. The antimicrobial effects of postbiotics were examined by disk diffusion, and postbiotics produced by 18 Lb. plantarum strains and 20 Lc. lactis strains showed strong antimicrobial activity. Significant differences in the antimicrobial effects of postbiotics were observed between the two species. Lb. plantarum postbiotics exhibited both bacteriostatic (concentration 60%) and bactericidal (concentration 80%) effects on ESBL-positive E. coli strains, whereas Lc. lactis postbiotics showed only bacteriostatic effects (80% concentration). Postbiotics derived from probiotic bacteria offer promising effects against multidrug-resistant E. coli due to their heat resistance, activity across different pH values, strong antimicrobial effects, affordability, and ease of production. Full article

T cells play an important role in the development and progression of type 1 diabetes (T1D). Checkpoint receptors regulate T cell activity, and their expression may be linked to the cells’ metabolic state. This study aims to investigate the association between T regulatory (Treg) and T conventional (Tconv) cells expressing various checkpoint inhibitors and glucose metabolism in type 1 diabetes patients and healthy controls (HCs). The study included 28 participants, with 16 of them diagnosed with type 1 diabetes, while 12 constituted a healthy control group. Multicolor flow cytometry, spectrophotometric analysis, and bead-based multiplex assays were utilized for the analyses. The study revealed that the most significant difference in T cell subsets in peripheral blood concerned TIGIT. Compared to healthy subjects, the percentages of TIGIT+ Tregs and TIGIT+ Tconvs were lower in T1D patients. Interestingly, hyperglycemia in in vitro cultures reduced percentages of TIGIT+ Tregs and TIGIT+ Tconvs, and to some extent also CTLA-4+ Tregs. A decreased percentage of these subsets was, in turn, associated with reduced glucose uptake and lower activity of the enzymes responsible for various stages of glucose metabolism. The described associations suggest a negative influence of hyperglycemia in T1D on immune regulation via a TIGIT-dependent mechanism. Hyperglycemia seems to reduce the percentage of highly regulatory TIGIT+ Tregs both in vivo and in vitro, and it is associated with reduced glucose consumption by these cells. At the same time, a reduction in the percentage of TIGIT+ Tconvs under such conditions may facilitate higher activity of Tconvs, including aberrant autoimmune reactions. Full article

Cyanobacteria, particularly Microcystis aeruginosa, can form dense blooms that impair water quality, and conventional treatment methods often fail to remove them effectively. This study evaluated the impact of cell age on the performance of the UV/H₂O₂ advanced oxidation process against M. aeruginosa. Cultures of M. aeruginosa were monitored over 64 days at an initial culture density of 1.20 × 10⁶ cells mL⁻¹. For the UV/H₂O₂ experiments, cells were adjusted to a density of 5.00 × 10⁵ cells mL⁻¹, and the growth and oxidative experiments were monitored using parameters such as hydrogen peroxide decay concentration, optical density at 730 nm (OD₇₃₀), cell density, and dissolved organic carbon (DOC). The hydrogen peroxide (H₂O₂) dosages used were 20 mg L⁻¹ and 50 mg L⁻¹, and the results showed that despite varying cell ages, H₂O₂ consumption remained stable at both dosages. While optical density and cell count indicate total cell removal, DOC levels increased due to cell lysis, resulting in contributions from both intracellular and extracellular fractions. A linear correlation was found between cell density and OD₇₃₀, and between total DOC and cell density. In conclusion, cell age did not influence the effectiveness of the UV/H₂O₂ process under the conditions tested. These findings indicate that UV/H₂O₂ can be an effective approach for managing cyanobacterial blooms in water treatment systems, with its performance being unaffected by cell age. Full article

This study explores the intersection of sociocultural factors, particularly privacy, with energy consumption patterns in residential buildings in Riyadh, Saudi Arabia. While cultural values around privacy have long been recognised as influential in residential design, the impact of these values on energy consumption is underexplored. This research aims to fill this gap by examining how privacy needs, residents’ preferences, and open layouts affect energy efficiency, particularly in terms of natural light and ventilation. A mixed-methods approach was employed, including semi-structured interviews with engineers, data collected from 108 respondents via an online survey, a case study of a residential building in Riyadh, and building performance simulations using IES software. The study also assessed actual energy consumption data and indoor lighting as potential implications of privacy concerns, causing changes in behavioural control of systems (e.g., windows, blinds, lighting, etc.). It focuses on the relationship between privacy needs, energy use, and natural daylight distribution. The IES simulation results for the studied residential building show an annual energy consumption of 24,000 kWh, primarily due to cooling loads and artificial lighting caused by privacy measures applied by the residents. The findings reveal that privacy-driven design choices and occupant behaviours, such as the use of full window shutters, frosted glazing and limited window operation, significantly reduce daylight availability and natural ventilation, leading to increased reliance on artificial lighting and air conditioning. This study highlights the need for human-centric design approaches that address the interplay between sociocultural factors, particularly reinforcing cultural sensitivity, and building performance, offering insights for future sustainable housing developments in Riyadh and similar contexts. Full article

Reducing the alcohol content of wines has received increasing attention, and various strategies have been proposed for this aim. In this study, non-Saccharomyces yeasts isolated from Uruguayan vineyards were screened to identify strains with low ethanol production for use in mixed cultures. Twenty-six strains belonging to six species were evaluated, considering key oenological parameters such as ethanol and glycerol production, glucose and fructose consumption, and absence of organoleptic defects. Based on these criteria, three strains from two genera were selected: Starmerella bacillaris (Sb1 and Sb2) and Metschnikowia fructicola (Mf2). In pure cultures, Starmerella bacillaris showed high sugar consumption along with high glycerol production. Subsequently, co-inoculation and sequential inoculation conditions were tested by combining the selected strains with commercial Saccharomyces cerevisiae (Sc). With Mf2 + Sc sequential inoculation, high sugar consumption, increased glycerol production, and a significant reduction in ethanol were observed compared to the control. For Starmerella bacillaris, only Sb1 achieved consistent alcohol reductions in sequential strategies. With co-inoculation, both strains reduced ethanol by 0.2–1% v/v, although only Sb1 showed complete sugar depletion. Overall, the results demonstrate a marked dependence of fermentation behavior on the strain and highlight the importance of studying biocompatibility and inoculation strategy in mixed cultures. Full article

In recent years, the application of augmented reality digital technology in brands has transformed the way consumers interact with brands. This study focuses on the impact of augmented reality (AR) technology on consumption behavior and brand communication related to intangible cultural heritage products, integrating the TAM and UTAUT2 theories to construct a research model. This study employed a time–location sampling method, utilizing SPSS and AMOS software for data analysis based on valid questionnaires completed by 305 AR-experiencing consumers in Changsha City, Hunan Province. Results indicate that the presence and novelty of AR technology significantly and positively influence consumers’ attitudes toward using AR technology, which in turn affects their purchase intent, social media sharing behavior, and brand attitudes. The study confirms that emotional factors and consumer perceptions play a guiding and decisive role in the new consumption reality enabled by AR technology. These research findings have practical significance and value for ICH brand building and AR marketing, demonstrating that AR is an effective means to enhance the visibility and influence of the ICH brand. They inject new vitality into promoting more sustainable ICH protection and popularization, as well as the development of the digital creative industry. Full article

Today, PHB and its copolymers—potential plastic substitutes—are produced by fermenting sugar, which is not scalable to the volumes of plastic consumption. PHB from CH₄ can offer a sustainable process route, with CH₄ potentially produced from a variety of waste biomass streams through anaerobic digestion, gasification, and methanation. The high molar mass (M_w) of PHB is a key determinant of its mechanical properties, and strain, culture conditions and downstream processing influence it. In this work, the strain Methylocystis sp. GB 25 (DSMZ 7674) was grown on natural gas as the sole carbon and energy source and air (1:1) in a loop reactor with 350 L active fermentation volume, at 35 °C and ambient pressure. After two days of continuous growth, the bacteria were limited in P and N for 1, 2, and 2.5 days to determine the optimal conditions for PHB accumulation and the highest Mw as the target. The biomass was then centrifuged and spray-dried. For downstream processing, chloroform solvent extraction and selected enzymatic treatment were deployed, yielding ~40% PHB from the biomass. The PHB obtained by solvent extraction exhibited high average weight molar masses of M_w ~1.1–1.5 × 10⁶ g mol⁻¹. The highest M_w was obtained after one day of limitation, whereas enzyme treatment resulted in partially degraded PHB. Cold chloroform maceration, interesting due to energy savings, did not achieve sufficient extraction efficiency because it was unable to extract high-molar-mass PHB fractions. The extracted PHB has a high molar mass, more than double that of standard commercial PHB, and was characterized by DSC, which showed a high degree of crystallinity of up to 70% with a melting temperature of close to 180 °C. Mechanical tensile properties measurements, as well as dynamic mechanical thermal analysis (DMTA), were performed. Degradation of the PHB by enzymes was also determined. Methanotrophic PHB is a promising bioplastics material. The high M_w can limit and delay polymer degradation in practical processing steps, making the material more versatile and robust. Full article

Introduction: The COVID-19 pandemic had significant effects on mental health and lifestyle behaviours, especially among university students who experienced academic disruptions, social isolation, and fewer social interactions. Alcohol consumption has long been part of student culture. Still, the influence of post-pandemic academic reintegration on drinking patterns and psychological distress remains relatively unexplored, particularly in countries like Portugal, where student traditions heavily shape consumption habits. This study aimed to describe the prevalence of alcohol consumption, depression, anxiety, and stress in a sample of Portuguese university students during the post-pandemic academic period, and to explore associations with sociodemographic variables. Methods: A cross-sectional study was conducted in November 2021 with 90 students from a private higher education institution in northern Portugal. Data were collected via an online questionnaire including sociodemographic information, the Alcohol Use Disorders Identification Test (AUDIT), and the Depression, Anxiety and Stress Scale (DASS-21). Result: The majority of the participants were not at risk of alcohol addiction (95.3%). In total, 15.1% of students reported anxiety symptoms ranging from severe to extremely severe. A binomial logistic regression was performed to ascertain the effects of being away from home and psychological distress (DASS-42 score), on the likelihood that participants were at risk of alcohol addiction (Level 3 and 4 in the AUDIT scale). The logistic regression model was statistically significant, χ²(2) = 9.20, p = 0.010. Living away from home was associated with a substantially lower likelihood of high-risk status (B = −2.79, p = 0.034), corresponding to an odds ratio of 0.06, indicating a strong protective effect. DASS-42 total score was positively associated with high-risk status (B = 0.04, p = 0.039), such that higher psychological distress increased the odds of being classified as high risk. Conclusions: The findings reveal a low prevalence of alcohol risk but heightened symptoms of anxiety, depression, and stress. Psychological distress notably increases the likelihood of hazardous alcohol use, emphasising the importance of targeted mental health and alcohol-use interventions among university students. Full article

As sustainable tourism gains global momentum, extended reality (XR) technologies have emerged as important tools for enhancing visitor experiences at overburdened World Heritage Sites while mitigating physical deterioration through non-consumptive engagement. However, existing research on immersive technologies in heritage tourism has largely relied on single-cultural samples and has paid limited attention to theoretically grounded boundary conditions in post-adoption behaviour. To address these gaps, this study extends the Expectation–Confirmation Model (ECM) by incorporating cultural distance (CD) and prior visitation experience (PVE) as moderating variables, and empirically tests the proposed framework using a mixed domestic–international sample exposed to an on-site XR application at the Badaling Great Wall World Heritage Site. Data were collected immediately after the XR experience and analysed using structural equation modelling. The results validate the core relationships of ECM while identifying significant moderating effects. Cultural distance attenuates the positive effects of confirmation on perceived usefulness as well as the effect of perceived usefulness on continuance intention, while prior visitation experience weakens the influences of enjoyment and visual appeal on satisfaction. These findings establish important boundary conditions for ECM in immersive heritage contexts. From a practical perspective, the study demonstrates that high-quality, culturally responsive XR can complement physical visitation and support sustainable conservation strategies at large-scale linear heritage sites. Full article

The consumption of ultra-processed foods (UPFs) in adolescence is high due to their widespread availability and accessibility and has been linked to increased cardiometabolic risk. In the Autonomous City of Melilla, an environment with particular cultural and religious characteristics, it is relevant to analyze the relationship of UPFs with metabolic markers of type 2 diabetes mellitus. This is a cross-sectional pilot study on 31 Muslim adolescents aged 15 to 17 years. The NOVA food classification was used to identify UPFs. The final sample comprised Muslim adolescents because written consent for venous blood sampling was obtained only from Muslim families/legal guardians. Separate multiple linear regression models adjusted for sex were fitted to examine the associations between UPF intake (%E/day) and each cardiometabolic and inflammatory marker. Higher UPF intake was positively associated with BMI, body fat percentage, waist circumference, waist-to-height indicator (ICA), and fasting glucose after controlling for the false discovery rate (q < 0.05). Regarding the inflammatory component, Muslim girls had elevated levels of IL-7, IL-10, and IL-13, and Muslim boys had higher levels of MIP-1β. In addition, IL-8 correlated positively with waist circumference, BMI, and the HDL/LDL ratio, while MCP-1 was negatively associated with Apo A1, total cholesterol, and HDL. In this exploratory pilot study, higher intake of UPF appears to be associated with greater central adiposity and higher fasting glucose; these hypothesis-generating findings warrant confirmation in larger, representative samples and may inform culturally adapted nutritional screening in Melilla. Full article