Search Results (248)

This study examines how mothers raising children with disabilities in American Samoa experience the processes of seeking diagnosis, navigating special education, and advocating for services within an insular rural context. American Samoa, an unincorporated U.S. territory located 2600 miles from Hawaiʻi with a population under 50,000, represents a case of what we term insular rurality—a condition in which the structural disadvantages of rurality are intensified by oceanic isolation, territorial governance, and colonial history. Data were collected through three focus groups with fifteen mothers whose children hold a range of disability diagnoses, with a card sort activity at the outset of each session serving as an idiographic anchor to protect individual voice within the group format. Analysis followed Interpretative Phenomenological Analysis adapted for focus groups (IPA-FG), proceeding from line-by-line exploratory noting through Personal Experiential Themes and Group Experiential Themes within each focus group case to cross-case convergence and divergence analysis, interpreted through the Fonofale model of Pacific wellness. Findings reveal two overarching themes: systemic invalidation, in which mothers encountered deficit-based assumptions, stagnant educational goals, and institutional disengagement; and parent peer support as the primary infrastructure, in which mothers became de facto experts, built community-driven solutions, and envisioned more inclusive futures. Technology emerged as a contradictory force—valuable for parent learning but largely ineffective for children’s remote therapy. These findings suggest how workforce shortages and geographic isolation create conditions in which maternal advocacy becomes a systems-level necessity rather than a personal choice. Implications for rural education policy, IDEA implementation in U.S. territories, and culturally grounded family support are discussed. Full article

Plant cytochrome P450 monooxygenases (CYPs) are valuable biocatalysts for the regioselective hydroxylation of aromatic compounds. However, their expression in bacterial hosts is hampered by poor solubility, membrane anchoring and the requirement for redox partners. In this work, we report the design and characterization of modular expression systems that enable the functional production of SbCYP82D1.1 from Scutellaria baicalensis (SbF6H) in Escherichia coli. Both independent expression and synthetic fusion systems were evaluated by combining a CYP with a compatible reductase (ATR2_tr from Arabidopsis thaliana) to catalyze the conversion of chrysin into baicalein. A combinatorial library of N-terminal variants, host strains, media, and induction strategies was constructed and screened. Among the tested host, E. coli DH 10-beta provided the highest product titers, particularly when cultures were supplemented with 5-aminolevulinic acid. Truncation of the native transmembrane anchor significantly improved catalytic performance, whereas the addition of the heterologous MALLLAVF tag decreased activity. Fusion systems outperformed separate expression formats, showing approximately two-fold higher activity, with the flexible glycine–serine linker (L_GS) supporting the highest hydroxylation product formation. The corresponding fusion construct showed an apparent conversion of 0.1 mM chrysin to baicalein of up to 90% under the applied whole-cell reaction and analytical conditions, although this value should be interpreted with caution due to the concurrent instability of baicalein observed in all reactions and culture conditions. This result nevertheless indicates a marked improvement in whole-cell baicalein formation compared with previously reported bacterial systems. Together, these results demonstrate that rational N-terminal engineering combined with fusion protein design can enable efficient bacterial expression of plant CYPs, representing a promising step toward scalable production of hydroxylated flavonoids. Full article

Wikidata provides extensive coverage of tourism-related Points of Interest (POIs), yet its heterogeneous type system and uneven metadata limit its direct use in smart tourism applications. This paper presents an end-to-end pipeline that transforms Wikidata POIs into a compact and interpretable tourism-oriented representation supporting multi-category assignments. We collect POIs from six countries—Greece, Italy, Spain, Norway, Sweden, and Denmark—and construct a dataset that integrates core identifiers with textual descriptions, type information, heritage indicators, geographic coordinates, and Wikipedia sitelinks. We introduce an eight-category tourism taxonomy capturing key themes, including cultural venues, archaeological and historic sites, monuments, fortifications, religious sites, protected areas, natural features, and coastal or water locations. As a reproducible baseline, category likelihoods are estimated using sentence embeddings and similarity to category anchor descriptions, producing a probability vector for each POI. Building on this baseline, we propose a fuzzy inference layer that integrates embedding-based probabilities with structured Wikidata signals to generate interpretable membership degrees across categories and enable principled multi-category classification. This fusion is particularly valuable for smart tourism applications, as it supports robust faceted exploration and personalized recommendations (e.g., “historic + coastal”), while providing evidence-based explanations that enhance user trust and facilitate curator oversight when POI metadata is sparse or ambiguous. The resulting pipeline produces ranked POI catalogs by country and category, country-level tourism profiles, and diagnostic views for examining uncertain cases. The approach is fully reproducible and readily adaptable to other geographic regions or domain taxonomies. Full article

Background/Objectives: Western Canadian and U.S. communities outside urban centres remain underserved by primary, specialist, emergency, mental health, and chronic-disease services. These access problems reflect distance, weather, workforce shortages, specialist maldistribution, primary care attachment gaps, broadband limitations, and the governance realities of Indigenous and Tribal communities. This scoping review with narrative synthesis examined how telehealth and virtual-care models affect rural access in western Canada and the western/frontier United States. Methods: Searches were completed on 21 May 2026 in PubMed/MEDLINE, Embase, CINAHL, Scopus, the Cochrane Library, and PubMed Central. Supplementary searches included Google Scholar, publisher platforms, reference-list checking, and official Canadian and U.S. health-system sources. Peer-reviewed evidence published from 1 January 2016 to 21 May 2026 was eligible when it addressed rural, remote, frontier, Indigenous, underserved, western, or northern healthcare settings and reported access, implementation, safety, continuity, equity, or service-use outcomes. Results: The search identified 112 records; 27 duplicates were removed, 85 records were screened, 37 full texts were assessed, and 28 peer-reviewed records were included. Seven official sources were retained separately. Evidence was mainly observational, qualitative, mixed-methods, implementation-focused, or review-level. Moderate confidence supported telehealth for travel reduction and specialist input, especially through eConsultation, provider-to-provider consultation, telementoring, and real-time emergency support. Confidence was low to moderate for hybrid primary care and telemental health, and low for durable reductions in emergency department use. Conclusions: Telehealth may be most appropriately implemented as a hybrid, locally anchored, culturally safe access model, not as a stand-alone substitute for rural primary care, specialist capacity, or emergency services. Implementation should include broadband support, local physical assessment capacity, documentation, continuity, patient education, and clear escalation pathways. Full article

This qualitative study examines how Orthodox and ultra-Orthodox Jewish fathers in Israel describe raising a child with autism within the everyday life of a religious family. Although research on autism and family life has expanded, fathers’ voices remain underrepresented, especially in religious families in which family routines, the Sabbat and holidays, and everyday religious practices shape parenting and participation at home. Data were collected from 127 fathers of children aged 3 to 18 through an online Hebrew questionnaire that included open-ended questions and were analyzed using qualitative content analysis. The findings suggest that religiosity shaped fathers’ caregiving not only as a source of meaning, hope, and emotional strength but also as a practical framework for everyday accommodation and belonging. Four themes emerged: (1) religiosity as an anchor for resilience; (2) paternal love as a religious practice of accommodation; (3) paternal adaptation during the Sabbat and holidays; and (4) religious authority as a basis for legitimizing care practices and preserving the child’s place within family and religious life. Together, these findings underscore the importance of culturally responsive support that acknowledges how religious meaning systems shape paternal care, family participation, and children’s inclusion, while also suggesting that fatherhood may function as an ongoing mediating process within religious family life. Full article

Research on contemporary Chinese comprehensive material painting has long focused on aesthetic qualities or relied on Western modernist frameworks, leaving the mechanism of material-to-symbol transformation under-theorized. This study investigates the generative logic of material meaning through a qualitative cross-case analysis of forty-eight works by twelve Chinese artists, integrating in-depth interviews and visual analysis. Through systematic analysis of images, material operations, and artists’ interpretations, the study proposes a three-stage semiotic mechanism: anchoring, exemplification, and differentiation. Findings demonstrate that material meaning is formed through continuous negotiation between bodily technique and material resistance, mediated by cultural techniques guided by pre-textual schemata. Material attributes are first filtered and anchored, reinforced through embodied operations, and eventually stabilized into stylistic structures articulating cultural identity. This research argues that Chinese comprehensive material painting constitutes a localized mode of cross-cultural symbolic production rooted in indigenous cultural experience and material praxis. Ultimately, it supplies a mechanism-based interpretive framework for this art form while contributing a localized perspective to global discussions on the relationship among subjectivity, materiality, and cultural identity. Full article

This paper examines how artificial intelligence can support sustainability assessment in cultural industries, using national film industries as a test case. The Film Industry Sustainability Index (FISI) is introduced as a composite indicator covering cultural diversity, economic resilience, and Sustainable Development Goal (SDG) alignment for 42 national economies from 2005 to 2023. Knowledge-Enhanced Mamba (KE-Mamba), a selective state-space forecasting model, is then proposed to combine annual panel indicators with country-level film-industry knowledge graph (KG) embeddings and large language model (LLM)-derived screenplay-oriented narrative proxies from film synopses. To reduce factual errors in title-level narrative scoring, the LLM is anchored to verified United Nations Educational, Scientific and Cultural Organization (UNESCO) records and the European Audiovisual Observatory’s LUMIERE film-admissions database using rank-one model editing (ROME). On the 2020–2023 held-out test period, KE-Mamba achieves a composite FISI mean absolute error (MAE) of 0.0389, a mean absolute percentage error (MAPE) of 5.61%, and an $R^2$ of 0.934, outperforming autoregressive integrated moving average (ARIMA), tree-based, long short-term memory (LSTM), and base Mamba baselines. Additional robustness checks using a pre-pandemic split, two-way fixed-effects panel regression, alternative FISI weighting schemes, KG embedding ablations, and human validation of LLM narrative scores support the reliability of the proposed framework. Policy simulations are interpreted as model-based projected associations rather than causal estimates. The results show that knowledge-enhanced sequence models can provide transparent forecasting support for sustainable cultural-industry policy. Full article

Cariño (care) should be central to equity-centered transformation in science, technology, engineering, and mathematics (STEM) higher education. Yet, relational leadership practices that prioritize culturally grounded care—such as cariño—are often absent in STEM initiatives, leaving unexamined how Women of Color (WOC) enact these practices to advance equity for historically marginalized students. Employing a qualitative methodology grounded in Chicana Feminist Epistemology, in-depth interviews were conducted with five WOC leading a multi-institutional, federally funded STEM initiative. Analysis revealed four interrelated dimensions of what we are calling “Cariño Competence”: (1) relational attunement grounded in moral obligation, (2) protective action when project systems fail students, (3) boundary-setting as care and resistance to extractive labor, and (4) community-sustained resilience through networks of WOC leaders. The findings offer a data-driven theorization of Cariño Competence, capturing how WOC operationalize culturally grounded care as a strategic, protective, and resistive praxis. By centering students as the moral and epistemic anchor of leadership decisions, this study demonstrates how relational, culturally sustaining practices can humanize bureaucratic systems, buffer harm, and advance systemic transformation in STEM higher education. These insights contribute to scholarship on culturally responsive leadership and provide a practical framework for advancing equity, inclusion, and empowerment in higher education contexts. Full article

Empirical management of acute pyelonephritis in Eastern Europe is increasingly constrained by extended-spectrum β-lactamase (ESBL)-producing Enterobacterales and by uropathogen phenotypes—such as strong biofilm formation—which may further blunt antimicrobial activity. We aimed to characterise resistance mechanisms, minimum inhibitory concentration (MIC) distributions, biofilm-forming capacity, and the in vitro performance of carbapenem-sparing agents and to test whether these microbiological features improve prediction of clinical failure beyond standard bedside risk scores. We retrospectively analysed 102 Enterobacterales isolates recovered from 129 consecutive culture-confirmed adult pyelonephritis admissions at “Victor Babeș” University Hospital, Timișoara (March 2022–March 2025). MIC values were determined by Vitek 2 and interpreted using EUCAST v13 breakpoints; ESBL, AmpC, and carbapenemase phenotypes were confirmed by combination disk and modified carbapenem inactivation methods. Biofilm formation was quantified by the microtiter-plate crystal-violet assay. Mediation, Restricted Mean Survival Time (RMST), and decision-curve analyses were used to assess added clinical value. ESBL was confirmed in 30/102 (29.4%) isolates, AmpC in 9 (8.8%), and carbapenemase in 4 (3.9%). ESBL+ isolates were more often strong biofilm formers (33.3% vs. 12.5%; p = 0.014) and showed a 4- to 16-fold rightward MIC shift for cefepime, piperacillin–tazobactam, and ciprofloxacin. Among carbapenem-sparing agents, ceftazidime–avibactam (96.7% S), fosfomycin (80.0% S), and amikacin (73.3% S) retained the highest activity against ESBL+ isolates. Strong biofilm formation and the ESBL phenotype were independently associated with worse outcomes (adjusted OR 3.5 and 4.7); an exploratory mediation analysis suggested that biofilm formation may explain part of the observed association between the ESBL phenotype and treatment failure and that delayed effective therapy may account for a further portion of this association. A microbiology-enhanced model that added the ESBL phenotype, biofilm strength, and acquisition setting to routine clinical variables improved discrimination over a clinical-only baseline (AUC 0.89 vs. 0.71) and showed a higher net benefit on exploratory decision-curve analysis across the 10–40% threshold range. These predictive findings derive from a single-centre cohort with a small number of events and were only internally validated; they require validation in independent cohorts before any clinical application can be considered. The ESBL phenotype and strong biofilm formation were each independently associated with worse outcomes in pyelonephritis and may help identify candidate isolates for carbapenem-sparing strategies anchored on ceftazidime–avibactam, fosfomycin, and amikacin; given the observational, single-centre design, these associations should be regarded as hypothesis-generating. Full article

Heart failure (HF) remains a major cause of morbidity and mortality despite substantial therapeutic progress, and important phenotype-specific treatment gaps persist. Protein S-nitrosylation (SNO) is a reversible cysteine-centered post-translational modification (PTM) whose reported associations with selected HF-relevant contexts, including vascular–endothelial dysfunction, mitochondrial–energetic remodeling, Ca²⁺-handling abnormalities, and selected receptor- or stress-related signaling observations, are supported to varying degrees. In this review, we evaluate reported mechanisms that may regulate cardiac SNO and define the evidentiary boundaries that constrain interpretation across HF-relevant settings. Available studies suggest that altered SNO homeostasis is associated with selected HF-related processes, but the strength of support varies substantially across targets, phenotypes, and disease contexts. Many mechanistic observations derive from animal models, cultured systems, donor-based perturbations, or non-HF settings. These should, therefore, be interpreted as hypothesis-generating rather than as established mechanisms in human HF. We accordingly distinguish findings supported by human HF tissue or HF-relevant in vivo evidence from more preliminary observations and highlight the need for human, site-resolved, and, where feasible, quantitatively grounded datasets. Future studies should prioritize stronger tissue anchoring, better integration of circulating and myocardial readouts, and closer alignment between mechanistic claims and the strength of the supporting evidence. Full article

This study examines how early Soviet culture sought to render time perceptible, governable, and politically productive by translating Futurist “chrontology” into socialist “chronization.” Taking Aleksandr Kusikov’s 1922 polemic on the Berlin journal Veshch (Thing)—edited by Ilya Ehrenburg and El Lissitzky—as its point of departure, it reconstructs a “third-way” Futurism shaped by postrevolutionary transit and exile, in which anticipatory futurity was inseparable from an intensified orientation toward materiality, technique, and constructive making. Against the backdrop of modern heterochrony—where “times” proliferate and synchronization remains contested—the essay traces how Soviet actors devised new units, comparisons, and pedagogies for living in a temporally unstable present. Two case studies anchor the argument. First, the League of Time (1923–1925) promoted multiscalar time-management practices through campaigns, memos, and didactic graphics that treated clocks, routines, and efficiency as instruments of socialist subject-formation. Second, illustrated children’s books translated variegated temporal sensibilities into a pedagogy of images, making futurity legible via “thingful” traces that recoded the past as a catalogue of obsolete objects. Chronization thus appears as an ambitious cultural technology for producing a planned, dynamic, and productive socialist way of being. By foregrounding work, visualization, and material comparison, these projects converted temporal uncertainty into actionable, collective everyday socialist practice. Full article

Background/Objectives: From a phenomenological perspective, the body is not merely a biological entity but the primary medium through which we experience and interpret the world. This study aimed to understand the lived body experience of advanced physiotherapy students at a university in Cali, Colombia, exploring how significant life events are embodied and expressed. Methods: A qualitative phenomenological design was employed. Twenty physiotherapy students participated in a body mapping exercise within a mental health elective. Participants graphically represented sensations, emotions, and memories on a body silhouette using colors and symbols. Data from the resulting body maps were analyzed using a thematic analysis approach via a data extraction matrix to identify patterns in symbolic, chromatic, and narrative elements. Results: The analysis revealed that students consistently inscribe both traumatic and positive life events onto their body maps, illustrating a narrative of resilience. Specific colors and body parts were symbolically charged: black and red in the heart, head, and shoulders represented pain and emotional burden, while blue and green in areas like the hands and stomach signified stability and achievement. External symbols (e.g., landscapes, bicycles) served as emotional anchors or representations of personal growth. Conclusions: Body mapping proves to be a powerful technique for accessing the embodied, often non-verbal, narratives of students. It underscores that the body functions as a living archive of experience. Integrating such methodologies into physiotherapy education can significantly enrich professional training by fostering sensitivity to corporality as a lived, relational, and cultural phenomenon, thereby strengthening future clinicians’ holistic and humanistic competencies. Full article

Zinc oxide promotes poultry growth, but it tends to agglomerate. This necessitates high doses and leads to environmental contamination from unabsorbed, excreted zinc. Undigested zinc is excreted and can enter the food chain, increasing the probability of zinc residues in edible poultry tissues (muscle, liver, and eggs) and raising concerns for consumer safety. MOF-supported single-atom zinc catalysts (SAC) resolve agglomeration by atomic anchoring, enhancing bioavailability. High-temperature/high-pressure fixation of Zn²⁺ surfaces was confirmed by XRD, while FESEM revealed the corresponding surface morphology, collectively verifying SAC formation. SAC exhibited potent antimicrobial efficacy against key pathogens such as Salmonella typhimurium, Escherichia coli, and Staphylococcus aureus (MIC of 3.125 mg/mL, MBC of 25 mg/mL). Co-culture experiments further demonstrated that the antibacterial performance of SAC remained stable over a temperature range of 20–80 °C and a pH range of 2–8, thus exhibiting excellent thermal stability and gastrointestinal tolerance. In 7-day-old chicks, SAC alleviated S. typhimurium-induced inflammation, reduced bacterial adherence, upregulated claudin-1, preserved gut homeostasis, ameliorated tissue lesions, and increased the abundance of Lactobacillus in the cecum, demonstrating promising potential for poultry infection control. Full article

Convergence education promotes learning experiences that integrate science, technology, engineering, and mathematics (STEM) to address complex real-world problems. However, secondary teachers often report limited access to professional development (PD) and curricular resources that support transdisciplinary instruction. This exploratory case study examines how four secondary teachers (three chemistry; one engineering) made sense of a transdisciplinary PD model, Convergence Research in STEM and Tradition (CReST), that leverages cultural heritage artifacts (Renaissance frescoes) as boundary objects to connect chemistry, engineering, world history, and technology. Teachers participated in a four-day immersive PD experience in Firenze (Florence) and Pisa, Italy, that included site-based learning, interaction with conservation scientists, and structured reflection. Data included daily reflective journals during the PD and semi-structured interviews following the experience, focused on teachers’ reflections on CReST and its implications for their instructional thinking. Using inductive thematic analysis, we identified patterns in teachers’ meaning-making about convergence instruction and the pedagogical possibilities the artifact opened for their classrooms. Findings indicate that (a) the fresco and associated conservation practices functioned as shared reference points for cross-disciplinary connections; (b) teachers reported shifts toward problem-centered, artifact-anchored pedagogy; and (c) sustained collaboration and shared tools were viewed as necessary for extending learning beyond the immersive experience. These findings indicate early, self-reported shifts in instructional planning, including artifact-based entry tasks, problem-centered instruction, and integration of real-world conservation practices. Implications are offered for designing science teacher PD that uses boundary objects to support coherent, culturally grounded STEM integration. Full article

This study examined the critical sustainability challenge of regional demographic decline in South Korea by analyzing how young people’s mobility decisions are intricately influenced by structurally and socially constructed meaning systems. Countering strictly economic deterministic views, this research posited that youth out-migration is a complex socio-cognitive process mediated by social representations of place—collectively constructed and circulated meanings attached to regions. Applying a secondary analysis of Q-sort data from 24 undergraduate students at a regional national university, the study integrated Q methodology with Social Representation Theory to systematically identify youth typologies regarding regional identity, territorial stigma, and local extinction. Participants sorted 44 statements encompassing place attachment, local consumption, cultural experiences, and policy effectiveness. Rigorous factor analysis revealed four distinct perception typologies: identity-based strategic mobility, conditional leaving based on internalized success norms, re-anchoring toward alternative lifestyles, and skeptical leaving rooted in profound institutional distrust. The findings empirically demonstrated that identical structural constraints can produce highly divergent mobility trajectories—ranging from active retention to complete resignation—depending entirely on the region’s socio-cognitive representation. This study demonstrates that local extinction is not merely a demographic condition, but a socially constructed framework of meaning and an object of social representation that shapes youth perception typologies and mobility judgments. Accordingly, moving beyond conventional technical interventions, meaning governance, and strategic communication are needed to help reimagine regional futures. Full article