Search Results (572)

Fully prefabricated bridge construction technology has gradually replaced traditional cast-in-place methods, becoming a core approach for efficient, environmental, and intelligent construction of bridges in cities. However, due to city-embedded restrictions, i.e., large width for more lanes, prefabricated cap beams in municipal bridges often demand segmental prefabrication and multi-point lifting, which hinders the overall construction efficiency. Based on a real project, this paper proposes a vertical rotation construction method for the bridge substructure, achieving integrated vertical rotation and positioning of prefabricated cap beams and piers by designing rotating steel shoes at the bottom of piers. This approach avoids segmental lifting of extraordinarily heavy cap beams while eliminating the need for prestressing and grouting operations high above the ground. Subsequently, detailed finite element models for critical rotational components, such as steel shoe, hinge pin and lug plates, are established to verify the stress distribution under various rotation conditions. The results demonstrate that the proposed layout, consisting of four 60 mm diameters 40Cr steel hinge pins with Q345 steel shoe and lug plates, effectively controls the representative stress within yield under all conditions, leaving the 10° scenario most unfavorable. Compared with the 10° rotation scenarios, increasing the initial rotation angle to 20° reduces the most critical stress inside the steel shoes by up to 39%, while further increasing to 30° only produces an additional 1% stress reduction. The design parameters of auxiliary equipment for the vertical rotation process can provide valuable references for future engineering practices. Full article

Digital survey and three-dimensional modelling have become central tools for the documentation and interpretation of architectural heritage, yet their epistemic role in the study of early Christian architecture still requires critical assessment. This article examines three early Christian sacella in northern Italy—Sante Teuteria e Tosca in Verona, Santa Maria Mater Domini in Vicenza, and San Prosdocimo in Padua—in order to evaluate how digital models can support architectural analysis without replacing direct engagement with the material monument. The study combines terrestrial laser scanning, photographic documentation, point-cloud processing, orthophotos, and architectural drawings to investigate masonry evidence, spatial organisation, roofing traces, and volumetric relationships. The results show that digital models make it possible to compare architectural elements that are difficult to observe simultaneously on site, especially in relation to construction sequences, domed spaces, subsidiary vaulted compartments, and traces of earlier phases. At the same time, the analysis demonstrates that digital visibility remains partial, since material presence, light, scale, and bodily experience cannot be fully reproduced in a virtual environment. The article argues that digital models are most valuable when understood as interpretative mediators: tools that extend observation, support comparison, and encourage renewed attention to the architectural fabric itself. Full article

Natural bitumens are promising alternative hydrocarbon resources, but their high resin–asphaltene content and strong coke-forming tendency limit their efficient conversion into valuable liquid products. This study elucidates the molecular transformations of resin and asphaltene fractions during thermocatalytic upgrading of natural bitumens from the Beke and Munaily Mola deposits in West Kazakhstan. Cracking experiments were conducted at 450 °C for 60 min using thermal treatment, fly-ash-derived ferrospheres, and di-tert-butyl peroxide (DTBP) as a radical-generating additive. Elemental analysis, average-molecular-weight determination, and nuclear magnetic resonance (NMR) spectroscopy were combined with structural-group analysis to establish changes in the molecular architecture of the heavy fractions. Thermal cracking produced 68–74% liquid products, while DTBP increased the liquid yield to 70% for Beke bitumen and 87% for Munaily Mola bitumen and substantially suppressed coke formation. Cracking promoted extensive degradation of aliphatic and naphthenic fragments, dealkylation, cyclization, dehydrogenation, and aromatization, resulting in increased aromaticity and lower molecular weight of the asphaltenes. The average molecular weight of Beke asphaltenes decreased from approximately 2044 to 1003 amu in the presence of ferrospheres. Although ferrospheres enhanced asphaltene destruction, they increased coke formation under the investigated conditions. These findings demonstrate that radical stabilization is critical for directing heavy-component conversion toward liquid products and provide a molecular basis for optimizing catalytic upgrading of natural bitumen. Full article

Adult acquired flatfoot deformity (AAFD) results from the collapse of the medial longitudinal arch (MLA), often managed conservatively with foot orthoses. While 3D printing enables rapid and cost-effective production of insoles, the biomechanical impact of different printing materials remains unclear. This study combined material testing, finite element (FE) simulation, and compression testing to evaluate four materials commonly used for 3D-printed insoles: PLA (polylactic acid), resin, EVA (ethylene vinyl acetate), and TPU (thermoplastic polyurethane). Tensile tests provided material properties for FE modeling of balanced standing using a validated healthy foot model. Outcome measures included MLA deformation (navicular drop), plantar stress distribution, and von Mises stress within the insole. A commercial semi-custom insole was tested for comparison. PLA and resin exhibited high stiffness (2228 and 880 MPa) and reduced navicular drop by 55–60%, compared with <30% reductions by EVA and TPU. Plantar stress distribution shifted from forefoot and hindfoot toward the midfoot, with PLA increasing midfoot load share to 38%. Compression testing confirmed that PLA and resin exhibited greater arch support ability than the semi-custom insole within a 10 mm displacement threshold. Material stiffness strongly influenced the biomechanical performance of 3D-printed insoles. Within this static simulation baseline, PLA and resin provided superior arch stabilization and stress redistribution compared with EVA, TPU, and a semi-custom reference. These findings suggest that material choice is critical to optimizing 3D-printed orthotic support, providing a valuable biomechanical baseline to guide future orthotic designs for flatfoot. Full article

Building on our previous multi-configuration Dirac–Hartree–Fock (MCDHF) computational strategies tailored for the hyperfine structure (HFS) of low-lying levels in one-valence electron Sr II and Ba II ions and in two-valence electrons in a Ba I atom, we successfully extend these methodologies along the alkaline-earth elements to the lighter Ca II and heavier Ra I–II ions. MCDHF-recommended HFS constants, along with their uncertainty estimates, are reported for the first time and critically discussed. Where applicable, the Bohr–Weisskopf correction is applied to the HFS constants, and its effects are analyzed. This is particularly valuable for cases where no measurement is available such as for the [Rn]${6d }^2{D}_{3/2,5/2}$ levels in ^223,225Ra II, for the [Rn]${7s6d }^3{D}_{1,2,3}$ and ¹$D_2$ levels in ²²³Ra I, for the [Rn]$7s{6d }^3{D}_{2,3}$ and ${}^{1}D_2$ levels in ²²³Ra I, and for the [Rn]$7s{7p }^3{P}_2^o$ level in ²²⁵Ra I. In all cases, our MCDHF values agree with the ones found in the literature within our error bars. For the ${}^{2}D_{5/2}$ levels in Ca II and Ra II, the discrepancies with the experiment observed in Sr II and Ba II for the HFS A constant are not seen. Full article

Background: Autophagy plays a central role in maintaining cellular homeostasis, regulating growth and development, and responding to multiple stresses. Autophagy-related genes (ATGs) play critical roles in autophagy, yet their functional diversity in soybean (Glycine max) remains underexplored. Methods: Genome-wide identification of GmATG genes was performed using sequence similarity and domain-based searches against the Wm82.gnm4 reference genome, followed by characterization of physicochemical properties, chromosomal distribution, phylogenetic relationships, gene duplication, conserved motifs, gene structure, three-dimensional structural, and promoter cis-acting elements. Tissue-specific expression and multiple stresses response were examined using transcriptome data and profiled by RT-qPCR. Results: A total of 60 GmATG genes belonging to 20 subfamilies were identified in soybean. Gene family expansion was predominantly driven by fragment duplication (33 gene pairs), with the ATG8 family expanding to 12 members, and pan-genomic analysis uncovered prominent copy number variation (6–9 copies) in the ATG18 family. GmATG genes showed distinct expression patterns in response to multiple abiotic and biotic stresses. Specifically, GmATG18f was significantly induced by phosphorus deficiency in the low-phosphorus-tolerant soybean variety Nannong 94-156. GmATG8g, GmATG9d and GmATG13d showed a typical expression trend of initial increase followed by decrease, with expression levels peaking at 6–12 h after salt stress treatment. GmATG8g and GmATG9d were rapidly upregulated at the early drought stress stage, while GmATG13a maintained sustained upregulation. In response to Phomopsis stem rot, GmATG7a/8h/8i/11/13d/18e/18f displayed differential expression in resistant and susceptible soybean materials. Conclusions: This study systematically characterizes the composition, expansion and stress response patterns of the GmATG gene family, revealing functional differentiation among family members. The identified key candidate genes, including abiotic-stress-regulated GmATG8g/9d/13d/18f and biotic-stress-regulated GmATG7a/8h/8i/11/13d/18e/18f, provide valuable genetic resources for the molecular breeding of stress-tolerant soybean. Full article

Sustainable management of antibiotic fermentation residues is critical to mitigating environmental and public health risks associated with pharmaceutical waste. Gentamicin fermentation residue (GFR), a challenging antibiotic byproduct rich in nitrogen and organic matter, presents both environmental risks and untapped resource potential. Pyrolysis can convert GFR into high-value products (pyrolysis oil, gas, and char) and eliminate environmental risks through high-temperature treatment. In this study, the product yields, pyrolytic kinetics, and nitrogen transformation pathways at different temperatures (400–800 °C) were investigated to explore the pyrolysis behavior and mechanism of GFR. Results revealed that temperature strongly influenced product distribution: biochar yield was dominant at low temperatures 51.2 ± 0.4% at 400 °C, oil yield peaked at 600 °C 14.6 ± 0.4%, and gaseous products became prevalent above 700 °C, reaching an estimated 78.4 ± 0.2% at 800 °C by mass-balance difference. Furthermore, isoconversional kinetic analysis (FWO and KAS) yielded apparent activation energies of 226.9–249.4 kJ/mol over α = 0.5–0.8, with R² values of 0.946–0.976, indicating conversion-dependent, multi-step devolatilization behavior. For nitrogen transformation, elemental and spectroscopic analyses showed that nitrogen transitioned from unstable pyrrolic forms in raw GFR to more stable pyridinic and graphitic forms in biochar, enhancing its potential for catalytic and environmental applications. Meanwhile, gaseous nitrogen species such as NH₃ and HCN were released at different temperature stages, and a three-stage nitrogen transformation mechanism was proposed linking the decomposition kinetics to the nitrogen migration pathways. These findings highlight pyrolysis as a promising, controllable, and sustainable method to convert antibiotic residues into valuable energy products and functional materials, with its contribution to sustainable pharmaceutical waste management contingent on proper NH₃/HCN gas treatment. Full article

Hinge joints are critical structural components that connect precast girders and enhance the load-bearing capacity and serviceability of multi-girder bridges. Damage to hinge joints can significantly reduce the structural integrity of a bridge and may even lead to bridge collapse. This study numerically investigated the effects of hinge-joint damage on the mechanical performance and inter-girder connections of a hollow-slab girder bridge using a surface-based cohesive behavior model. Hinge-joint damage was simulated in the finite element software ABAQUS (version 2022) using bond-performance degradation at the slab–hinge joint interfaces. The effects of damage location and severity on the stress distribution within the hinge joints were evaluated. The results reveal that damage in two hinge joints produces stresses 15% higher than those caused by damage in a single hinge joint. This indicates a weak superposition effect among multiple damaged joints. Additionally, stress fluctuations at key points Nos. 1 and 2 are significantly greater than those at key points Nos. 3 and 4. These findings provide valuable guidance for improving the durability and crack resistance of hinge joints and designing and maintaining multi-girder bridges. Full article

Nuclear Factor Y, subunit A (NFYA) constitutes a family of transcription factors that play critical roles in plant growth, development and abiotic stress responses. Taxodium hybrid ‘Zhongshanshan’ (T. mucronatum × T. distichum) is a fast-growing tree species with high industrial value and remarkable flooding tolerance. However, the systematic characteristics and abiotic stress response patterns of the ThNFYA gene family remain unclear. In this study, a total of 11 ThNFYA genes were identified. The encoded proteins ranged from 67 to 372 amino acids in length, with predicted molecular weights between 16.84 and 40.12 kDa. Phylogenetic analysis classified plant NFYAs into four clades, with all ThNFYAs falling into clades I and IV. Expression profiling revealed tissue-specific patterns, with six members showing the highest transcript levels in the cambium. Multiple cis-acting elements associated with stress and hormone responses were detected in the promoter regions of ThNFYAs. Most ThNFYAs were differentially regulated under salt, drought, and flooding stresses. Notably, most clade IV members (ThNFYA3, ThNFYA4, and ThNFYA6-ThNFYA8) were downregulated in the wood under partial submergence. This indicates their potential role in modifying wood properties in response to flooding. Co-expression network analysis identified ThNFYA1 and ThNFYA8 as central hub genes in leaves under partial submergence. Overall, these results suggest that the ThNFYA family may serve as candidate regulators of development and stress adaptation in T. hybrid ‘Zhongshanshan’. This study provides valuable insights for further functional verification of ThNFYAs and lays a foundation for marker-assisted breeding of stress-tolerant varieties. Full article

During forest exploitation, large amounts of wood biomass are left as residues after harvesting, even though this material could be valuable in reducing greenhouse gas emissions by replacing fossil fuels and serving as a source of bioactive compounds. Within the context of the European Union’s circular bioeconomy strategy, enhancing the efficacy and rational application of forest logging residues constitutes a critical future objective. To evaluate the possible use of the stumps as forest residue-derived woody biomass for the first time, the chemical composition and presence of bioactive compounds in the xylem and bark of F. angustifolia Vahl. ssp. pannonica Soó & Simon stumps were analyzed, including the content of cellulose, acid-insoluble lignin, acid-soluble lignin, ash, and extractives soluble in toluene–ethanol and hot water. In hot water extracts, total phenolic content, the antioxidant activity determined by scavenging capacity toward DPPH and HPTLC, the content of elements by ICP-OES, and the identification of bioactive compounds by UHPLC-DAD-ESI MS/MS were assessed. Considering the chemical composition, the quality of the xylem of the F. angustifolia (cellulose: 42.46 ± 0.45%; lignin: 25.05 ± 0.02%; hemicelluloses: 19.74 ± 0.46%) was similar to that of the stem. Hot water extracts of the bark of F. angustifolia stumps showed high total phenolic content (33.94 ± 1.43 mg GAE/g DW) and strong antioxidant potential (198.48 ± 3.33 μmol TE/g DW), while toxic metals were not detected. UHPLC-DAD-ESI-MS/MS analysis indicated phenylethanoid glycosides as the dominant compounds in the extracts. The analyzed stumps of Fraxinus angustifolia Vahl. in the “Morović” Forest Administration represent a valuable biomass with potential for further valorization. Full article

Recycling of lithium-ion batteries (LIBs) remains challenging due to high energy requirements, losses of key elements like lithium, and the heterogeneity of waste streams arising from different cathode chemistries. This study evaluates multiple recycling methods for LIBs black mass (BM), to recover critical raw materials and other valuable components. Three types of BM including nickel–manganese–cobalt (NMC), lithium iron phosphate (LFP) and a heterogeneous mixture of cell phones and laptops (HL; German: Handy/Laptops), were treated using froth flotation, pyrometallurgy, and biohydrometallurgy and their respective recovery efficiencies were assessed. The flotation results revealed that the HL sample had the lowest mis-recovery of non-ferrous metals into the froth product (around 10%), leading to further flotation only for HL. During screening, 94–99% of iron, phosphorus, and carbon in LFP-type BM were recovered in the fine fraction (<45 µm), while 92–99% of lithium, cobalt, manganese, nickel, and carbon in NMC-type BM were recovered in the same fraction. During precipitation, 99% of iron and 100% of phosphorus were recovered from LFP bioleachates at pH 3, while ~97–100% of dissolved cobalt, manganese, and nickel were recovered from NMC bioleachates. These findings confirm that no single recycling method is optimal for all battery chemistries. Full article

Field high-temperature and high-humidity (HTH) stress causes soybean seed deterioration, including shrinkage, moldiness, browning, and reduced germination rate, thereby decreasing seed vigor, emergence performance, and commercial value. Clarifying the molecular mechanisms of seed vigor formation under HTH stress is critical for identifying key genes and improving spring soybean seed quality. Plant homeodomain (PHD) proteins are conserved zinc-finger transcription factors involved in chromatin remodeling and stress responses. GmPHD3, a soybean PHD family member, has only been studied via heterologous expression in Arabidopsis; its authentic function in soybean remains unclear. In this study, we functionally characterized GmPHD3 in soybean. GmPHD3 is a nuclear-localized transcription factor containing Alfin and PHD domains, and is evolutionarily conserved across legumes and other plant species. Expression analysis showed that GmPHD3 was strongly induced by HTH stress, with rapid induction at 6 h in the tolerant cultivar Xiangdou No. 3 but delayed induction at 48 h in the sensitive Ningzhen No. 1. The GmPHD3 promoter harbors abscisic acid (ABA) and stress-responsive elements, and GUS assays confirmed its response to ABA and HTH. Overexpression of GmPHD3 in soybean significantly enhanced seed vigor under artificial accelerated aging by increasing the germination speed and activities of SOD, POD, while reducing TBARS content. Meanwhile, GmPHD3 negatively regulated seed longitudinal elongation, leading to a short and round seed shape without changing single-seed weight. It also significantly decreased the proportion of palmitic acid, a major saturated fatty acid. These results demonstrate that GmPHD3 positively regulates soybean seed vigor and stress resistance under HTH stress by modulating the antioxidant system and modifying seed morphology and fatty acid composition, providing a valuable target for soybean molecular breeding. Full article

NCED proteins play a critical role in drought, salt, and cold stress responses through ABA biosynthesis in plants. However, little information is currently available regarding the NCED gene family in Brassica species. Twenty-six putative BjNCED genes were identified in the genome of Brassica juncea, and found to be distributed on 15 chromosomes. Phylogenetic analysis suggested that these members could be classified into six subfamilies. The putative cis-elements were identified in the promoter regions of these BjNCED genes, and thought to be related to phytohormones, light, and abiotic stress responses. qRT-PCR analysis of five genes (BjNCED1, BjNCED4, BjNCED7, BjNCED16 and BjNCED18) revealed that, under salt stress, BjNCED16 and BjNCED18 showed transient induction, whereas exogenous ABA produced gene-specific and time-dependent expression patterns, including pronounced induction of BjNCED16 at 24 h. Low-temperature treatment strongly induced four of the five examined genes at 6 h. Analysis of the BjNCED genes in this study provides a valuable foundation for future investigations into the functional roles of the BjNCED family in response to growth, development and stress. Full article

Biosensors have become important analytical platforms that enable rapid, selective, sensitive and portable analysis for early disease diagnosis, biomarker monitoring and point-of-care diagnostic applications. Their analytical performance depends on the coordinated function of molecular recognition elements, surface chemistry, transduction mechanisms and signal-processing strategies. Nevertheless, the analysis of real biological samples remains challenging because of low target concentrations, matrix effects, interfering species, signal noise, sensor drift and device-to-device variability. Therefore, artificial intelligence and machine learning are gaining increasing importance as data-driven tools for signal preprocessing, calibration, feature extraction, pattern recognition, quantitative prediction and diagnostic decision support. These approaches are particularly valuable for interpreting complex datasets generated by electrochemical, optical, wearable and microfluidic biosensors. This review presents an overview of healthcare-oriented biosensor systems beginning with molecular recognition principles, bioreceptor design, and transduction technologies, and extending to applications in clinical diagnosis and health monitoring. It also examines the roles of supervised, unsupervised and deep learning approaches in biosensor data analysis, while critically discussing model validation, generalizability, interpretability and clinical translation. By linking molecular-level recognition with computational signal interpretation, this review highlights the advantages and limitations of artificial intelligence-integrated biosensors for next-generation point-of-care diagnostics, continuous health monitoring, and personalized healthcare applications. Full article

The development of efficient visible-light-driven semiconductor photocatalysts is essential for scalable and sustainable green hydrogen production. Among ternary metal oxides, MV₂O₆ (M = Zn, Ni, Cu, Mn, Co, etc.) metavanadates have attracted considerable interest because of their narrow band gaps (~1.8–2.5 eV), strong visible-light absorption, and unique edge-sharing VO₆ octahedral framework that promotes charge separation. This review summarizes recent advances in the design, synthesis, and electronic engineering of MV₂O₆-based photocatalysts for solar water splitting. Since direct particulate overall water splitting has only been demonstrated for MnV₂O₆, whereas ZnV₂O₆, NiV₂O₆, and CuV₂O₆ have mainly been investigated as photoelectrodes, both particulate photocatalytic and photoelectrochemical (PEC) systems are critically examined. The review clearly distinguishes these two configurations, highlighting how PEC studies provide valuable insights into charge transport, interfacial processes, and reaction kinetics while recognizing the additional challenges associated with suspension-based photocatalysis. Fundamental crystal structures, electronic band alignments, and charge-transfer characteristics of MV₂O₆ compounds are discussed, followed by recent advances in synthesis strategies, including hydrothermal, sol–gel, and deep eutectic solvent (DES)-assisted methods, together with morphology and defect engineering. Particular attention is given to oxygen-vacancy formation and its influence on visible-light absorption and charge separation. Modification strategies, including elemental doping, cocatalyst loading, and the construction of Z-scheme and step-scheme (S-scheme) heterojunctions, are critically evaluated for improving photocatalytic efficiency. Finally, the review discusses the key challenges that limit practical applications, including unfavorable band-edge positions, rapid carrier recombination, sluggish surface reaction kinetics, photostability, and the need to establish composition–structure–activity relationships. Future perspectives emphasize rational materials design through advanced characterization, theoretical calculations, and scalable synthesis approaches to accelerate the development of efficient MV₂O₆ photocatalysts for solar-driven hydrogen production. Full article