Search Results (749)

Continuous greenhouse watermelon cultivation is widely constrained by declining soil function, impaired nutrient cycling, and increasing soil-borne disease pressure. Developing biologically driven strategies to restore soil–crop coupling is therefore critical for sustainable protected horticulture. Here, we conducted a two-year field experiment (2024–2025) using a randomized block design with three treatments (CK, ST, and STE), three replicates per treatment, and a plot area of 22.5 m² to evaluate how straw application alone and in combination with earthworms regulate soil processes and crop performance in a continuous greenhouse watermelon system. Compared with CK and ST, earthworm–straw co-application (STE) exerted stronger effects, particularly during the mid-to-late growth stages. In 2024, STE increased soil organic matter by 25.34% and 30.28% relative to CK at the fruiting and harvest stages, respectively; in 2025, the corresponding increases were 25.22% and 27.62%. STE also significantly increased total nitrogen at nearly all growth stages, with the maximum increase reaching 67.23% relative to CK at harvest. In 2025, total phosphorus under STE was significantly higher than under CK and ST across all growth stages, with increases of 75.82% and 79.63%, respectively, at the fruiting stage. Neutral phosphatase activity was markedly enhanced, increasing by 292.24% at the fruiting stage in 2025. These improvements were accompanied by higher plot yield and lower wilt disease incidence, with yield increasing by 34.00% in 2024 and 21.29% in 2025 relative to CK, while disease incidence decreased by 41.46% and 56.06%, respectively. Integrative Mantel tests showed that total nitrogen was the factor most strongly associated with watermelon yield, with the correlation coefficient increasing from r = 0.490 (p = 0.001) in 2024 to r = 0.662 (p = 0.001) in 2025. Co-occurrence network analysis further revealed a strong positive correlation between yield and total nitrogen (r = 0.848 in 2024; r = 0.673 in 2025) and a negative correlation between disease incidence and total nitrogen (r = −0.661 in 2024; r = −0.822 in 2025), indicating progressively strengthened soil–plant functional coupling over time. Our findings demonstrate that earthworm–straw co-application strengthened soil nutrient transformation capacity and enhanced soil suppressiveness against wilt disease, thereby providing an effective ecology-based strategy for alleviating continuous-cropping constraints in greenhouse watermelon systems. Full article

Successive cropping frequently causes a decline in Chinese Fir (Cunninghamia lanceolata) biomass, a problem intricately tied to soil nutrient shifts and microbial processes. This research investigates the mechanisms governing biomass carbon partitioning and soil nutrient shifts in these plantations. This study investigated five Chinese Fir clones (‘ck’, ‘b44’, ‘K13’, ‘F13’, and ‘kt13’) across two cultivation regimes: continuous cropping (second-generation plantation, G2) and first-generation plantation (G1). The focus was on their biomass and soil nutrient status. The results showed that: (1) The biomass of different Chinese Fir clones at 25 years of age decreased significantly with increasing generations of continuous cultivation. Tree height showed no significant differences among clones within the same generation; however, the G2 cultivation significantly inhibited diameter at breast height (DBH). (2) The changes in soil nutrients and microbial activity under different successive generations (G1, G2) was closely linked to the decline in Chinese Fir biomass carbon. Analysis revealed that the decreases in dissolved organic carbon (DOC), dissolved organic nitrogen (DON), and Catalase (CAT) activity were significantly positively correlated with the reduction in biomass carbon. Concurrently, the decrease in soil pH showed a significant negative correlation with microbial biomass carbon (MBC) and Sucrase (SUC) activity. (3) Regarding growth traits, although tree height showed no significant differences among clones within the same generation, DBH was generally and significantly inhibited under G2 cultivation. An exception was the ‘K13’ clone, which remained largely unaffected. In terms of carbon accumulation, G2 cultivation led to a universal decline in biomass carbon across clones; however, the magnitude of reduction in different components (leaf, branch, stem, root) and total biomass carbon varied clone-specifically. Notably, ‘K13’ exhibited the strongest tolerance, with a significantly smaller decrease in tree biomass carbon compared to the other four clones, which showed substantially lower tree carbon stocks across all components relative to G1 plantations. This indicates that successive cropping of Chinese Fir likely constrains the carbon sequestration capacity of plantations by altering soil nutrient properties, thereby suppressing tree DBH growth and biomass carbon accumulation, likely through reduced net primary productivity. Among the five clones, ‘K13’ was the least affected, demonstrating its high potential for adaptation to continuous cultivation. These findings provide implications for sustainable forest management by guiding clone selection to mitigate productivity decline under successive cropping. Full article

Dryland farming systems in South Dakota face rainfall variability and rising water demand, which can reduce crop productivity and threaten long-term soil health. We combined field experiments across three dryland sites in South Dakota (Roscoe, Selby, Fort Pierre) with continuous soil moisture monitoring (0–15, 15–30, 30–45 cm) and HYDRUS-1D modeling to evaluate cover crops and soil amendments (biochar, manure) on water retention. During the active cover crop growth period, plots with cover crops consistently exhibited lower soil water content than plots without cover crops, likely due to increased transpiration. Plots with no cover crop (NCC) retained more water than cover crop (CC) plots (Roscoe: 26.27% vs. 24.16% at 0–15 cm). During the primary crop growing season, biochar consistently increased soil moisture (θ) compared with manure and unamended plots. Following a 43-day dry spell (1 July–13 August 2024), soil moisture declined by approximately 0.096 m³ m⁻³ in the biochar plots, compared with 0.125 m³ m⁻³ under manure and 0.216 m³ m⁻³ in the unamended control, exhibiting differences in water retention capacity among treatments. HYDRUS inverse modeling reproduced observed soil moisture dynamics (R² ~ 0.91) and demonstrated higher water content under biochar. Scenario analysis using representative wet (2008) and dry (2012) years showed the cover crop + biochar combination maintained the highest average water content. Results support integrating biochar with cover cropping to buffer drought and improve soil water availability in dryland farming. Full article

Livestock manure amendment improves soil fertility and promotes carbon sequestration, but long-term application leads to heavy metal (HM) accumulation with unknown ecological consequences. Based on a 13-year field experiment in a continuous maize cropping system, we compared chemical fertilizer (NPK) with four organic amendments (cattle, pig, chicken manure, and compost) applied on an isocarbon basis. Organic amendments significantly increased total organic carbon (TOC) by 15.8–24.3% and available phosphorus (AP) by 1.9- to 6-fold relative to NPK. Compost achieved the highest maize yield. However, pig and chicken manure led to substantial accumulation of Cu and Zn due to high background levels. Despite this, grain HM concentrations remained below safety thresholds, indicating no immediate food chain risk. Metagenomic analysis revealed that HM stress acted as a deterministic filter on the soil microbiome. Cattle manure fostered the most complex co-occurrence network (average degree: 2.70), while pig manure reduced network complexity and increased modularity (>0.92), reflecting a shift toward fragmented, survival-oriented interactions. This structural reorganization was coupled with functional shifts, including enrichment of stress-tolerant taxa (Chitinophagales, Nitrosotalea) and detoxification pathways. We recommend prioritizing cattle manure or compost over raw pig and poultry manure to balance fertility, productivity, and ecological safety in black soil regions. Full article

Remote sensing has become a cornerstone of modern agricultural monitoring, addressing the dual challenges of increasing production while ensuring environmental sustainability. Based on a conceptual framework developed over the past decade, key application areas include yield estimation, phenology, stress assessment (e.g., drought), crop mapping, and land-use change detection. In Central Europe, regionally specific conditions such as fragmented land ownership, small and irregular plots, and high climate variability shape these applications. Annual field crops, such as cereals, oilseeds, maize, and forage crops dominate production and represent the primary focus of monitoring efforts. Optical data from Sentinel-2 are effective for mapping crop types and analyzing phenology, especially when dense time series are available. However, persistent cloud cover during critical growth phases limits the effectiveness of optical approaches, prompting the integration of radar data from Sentinel-1. Multi-sensor strategies increase the robustness of classification and temporal continuity, supporting monitoring under adverse conditions. Reliable reference data from systems such as the Land Parcel Identification System enables parcel-level validation and facilitates object-oriented analyses in line with management needs. Future developments will increasingly rely on advanced time-series analysis, machine learning, and the integration of agrometeorological and crop model data. As climate change intensifies drought frequency and yield variability, remote sensing will play a pivotal role in enabling near-real-time monitoring and decision support within the evolving landscape of digital agriculture ecosystems. The aim of this review article is to provide an overview of crop monitoring in the Central European region over approximately the past fifteen years, emphasizing trends in subsequent technological and procedural developments. Full article

Natural rubber (NR) is essential to the medical, industrial, defense and transportation industries. Alternative rubber crops are needed to supplement increasing rubber demands which cannot be met by the tropical rubber tree, Hevea brasiliensis, and to protect supplies in the event of a rubber tree crop collapse, political strife or a pandemic disrupting global rubber supply chains. Taraxacum kok-saghyz, rubber dandelion, has high-molecular-weight NR, substantial rubber content and the ability to grow in temperate regions. It can also grow hydroponically or aeroponically in controlled environments. This work presents a scenario-based cost analysis of requirements to scale up hydroponic rubber dandelion to replace the 1 million metric tons of imported rubber consumed annually by United States manufacturers. Two scale-up scenarios were considered: a single-level, deep water culture greenhouse and an indoor, ten-level hydroponic vertical farm built in a warehouse. Fuel usage, operating costs, electricity consumption, beneficial insect applications, fertilizers, cooling, and more were included for each case. The costs of operation and construction were compared to the value of products to determine potential annual profit. Sensitivity analyses revealed several scenarios which would drastically improve the economics of the hydroponic facilities. A combination of multiple factors may allow economic feasibility. Hydroponic rubber dandelion production can be profitable on a small scale (up to 15 MT of TNR/year) provided leafy greens and inulin are included as coproducts. The validity of scaling up such a system to 100,000 MT TNR/year to meet 10% of US manufacturing requirements depends heavily on successful research-based gains in TNR concentration and root size, the difference in TNR price between a commodity price and a specialty NR, and upon whether or not tropical rubber tree NR is able to continue to provide a stable source of NR for the US. Full article

Adequate fertilization with nitrogen (N) and potassium (K) is essential to sustain crop productivity and minimize nitrogen losses to the environment. However, the increasing imbalance in fertilizer use in Poland, with low potassium inputs, may impair long-term soil fertility and nitrogen use efficiency. The aim of this study was to evaluate the effects of long-term potassium omission on maize productivity and nitrogen use efficiency under varying nitrogen fertilization rates. A 16-year field experiment (2003–2018) was conducted in two different regions of Poland (Grabów and Baborówko), on soils with medium to low available potassium content. Maize response to potassium fertilization cessation was evaluated at increasing nitrogen rates (50–250 kg N ha⁻¹). Grain yield, nitrogen uptake (Yn), nitrogen surplus (Ns), and nitrogen use efficiency (NUE) were assessed according to the EU Nitrogen Expert Panel (EUNEP) approach. Potassium omission had little effect on maize yield and NUE indices. At nitrogen rates commonly applied in agricultural practice in Poland (~100 kg N ha⁻¹), NUE strongly exceeded 100%. The other NUE indices—nitrogen surplus and nitrogen uptake remained optimal (<80 kg N ha⁻¹, and >80 kg N ha⁻¹, respectively), regardless of potassium treatment. These results indicate a low risk of nitrogen losses under moderate nitrogen inputs, even without potassium fertilization. However, given the declining NUE trend on soils with low potassium content, a reduction in nitrogen use efficiency can be expected, particularly under high nitrogen application rates and continued unbalanced potassium fertilization. Full article

White mold caused by Sclerotinia sclerotiorum (Lib.) de Bary continues to threaten yield and quality and remains a stubborn, sometimes unpredictable constraint in many cropping systems. The pathogen’s broad host range and its capacity to persist for years as sclerotia mean that fields can carry risk long after visible symptoms fade. Disease development is often driven by short windows of favorable temperature and moisture that promote germination and ascospore release and dispersal, while myceliogenic infection from soil-borne sclerotia can also initiate disease directly. Yet dependable control is still undermined by durable inoculum, limited stable host resistance, variable biocontrol performance, and shrinking chemical options together with fungicide resistance risk. Here we consolidate current understanding and ongoing uncertainties around sclerotial formation and germination cues, the environmental drivers that shape epidemic onset, and the processes governing host colonization, including the roles of cell wall-degrading enzymes, oxalic acid, and redox regulation, as well as the continuing debate over necrotrophic versus hemibiotrophic phases. Management is considered from a practical perspective, covering cultural risk reduction, forecasting-guided fungicide programmes supported by resistance-management principles, and biological control strategies targeting sclerotia. Across systems, the evidence points to the same lesson: single tactics rarely remain reliable under field variability, whereas integrated packages that reduce soil inoculum and align interventions with risk are more durable. Future priorities include resolving early infection events, improving prediction of carpogenic germination under changing climates, increasing the consistency of biocontrol, and accelerating resistance breeding supported by genomic resources. Full article

This study evaluated the performance of a smart farming technology (SFT) and a climate-smart agriculture (CSA) approach for improving irrigation management in pecan (Carya illinoinensis) orchards in México through soil moisture monitoring, evapotranspiration estimation, and real-time data integration. Continuous monitoring allowed irrigation to be maintained at field capacity, preventing plant stress while avoiding total soil saturation or permanent wilting point. Calibration of soil moisture sensors showed a very strong correlation (R² = 0.99) between sensor reverse voltage and volumetric soil water content in predominant sandy loam soils, confirming the reliability of the monitoring system for irrigation scheduling. Seasonal analysis of reference evapotranspiration (ETo) and crop evapotranspiration (ETc) revealed increasing atmospheric water demand during summer months, with crop coefficient (Kc) values ranging from approximately 0.3 during dormancy to 1.0–1.3 during peak vegetative growth. After five years of field implementation of the technology, results showed water savings exceeding 50% compared with traditional flood irrigation practices. The optimized irrigation schedule reduced total seasonal irrigation depth from 216 cm to 128 cm, representing a 59% reduction in applied water while maintaining adequate soil moisture conditions for crop development at field capacity (FC). These results highlight the potential of integrating sensor-based monitoring, evapotranspiration modeling, and IoT platforms to enhance water-use efficiency and support sustainable pecan production under increasing climate variability. Full article

The yellow peach moth, Conogethes punctiferalis, is a destructive polyphagous pest and poses a severe threat to the fruit industry and field crops worldwide with its continuously increasing population and expanding host range in recent years. Despite the severe damage caused by C. punctiferalis larvae, their antennae and mouthparts, equipped with abundant sensilla responsible for feeding behavior, have not been investigated in detail. In our study, the antennae, mouthparts, and associated sensilla of first-instar and mature larvae of C. punctiferalis were examined with light and scanning electron microscopy. Our results revealed no obvious morphological differences between the two instars in the basic composition of the antennae and mouthparts, or in the types, distribution, and numbers of sensilla. The antenna is three-segmented, with no sensilla on the scape, three sensilla basiconica and two sensilla chaetica on the pedicel, and three sensilla basiconica and one sensillum styloconicum on the flagellum. The mouthparts of C. punctiferalis are typically mandibulate and consist of a labrum-epipharynx, paired mandibles, a pair of maxillae, a labium, and a hypopharynx. Six types of sensilla were primarily concentrated on the labrum-epipharynx, maxilla, and labial palp, including sensilla chaetica, sensilla basiconica, sensilla styloconica, sensilla digitiformia, sensilla epipharyngea, and sensilla placodea. We conducted a systematic analysis of the characteristics of sensilla and discussed their variation in the context of Lepidoptera phylogeny. The potential functions of the sensilla have also been inferred. The study could advance our understanding of the behavioral ecology of C. punctiferalis and provide potentially useful information on the development of pest control technologies. Full article

To elucidate the regulatory mechanisms underlying the interaction between straw return and nitrogen (N) fertilization on yield formation, nutrient uptake, and soil N cycling in a continuous maize cropping system, a two-year positioning experiment was conducted. The study established two straw treatments (S0: 0 g/box; S1: 84 g/box) combined with three N levels (N0: 0 g/box; N1: 1.24 g/box; N2: 2.47 g/box). (The box refers to the cylinder used for planting maize.) The responses of maize yield, plant nutrient accumulation and partitioning, fertilizer-derived N ratio, nitrogen fertilizer use efficiency (NUE), and soil microenvironment were analyzed. Results indicated that under N1 conditions, straw return had a negligible effect on crop growth and yield formation. Conversely, under N2 conditions, straw return significantly enhanced maize yield and promoted the accumulation of N, phosphorus (P), and potassium (K) in plant tissues. ¹⁵N isotope tracing revealed a novel mechanism: rather than significantly altering direct fertilizer nitrogen use efficiency, straw return improved crop yield primarily by elevating indigenous soil N content and boosting the activities of N-transforming enzymes, thereby beneficially altering the ultimate environmental fate of the fertilizer N. Furthermore, straw return significantly boosted the activities of enzymes involved in N transformation and optimized the soil microenvironment. Collectively, straw return coupled with increased N application (specifically the S1N2 treatment) significantly maximizes maize yield, providing a theoretical basis for rational straw utilization and N management. Full article

This study examined nectar and pollen production as well as pollinator visitation in Nigella damascena (Ranunculaceae), an annual ornamental and seed crop, over two flowering seasons. Flower anthesis lasted 6–7 days, with protandry: the male phase began on the first day, and pollen presentation continued until corolla senescence. Peak stigma receptivity occurred in 5-day-old flowers, resulting in a partial overlap of male and female functions between days 5 and 7. Nectar was secreted by petal-derived structures, with secretion beginning in 1-day-old flowers and steadily increasing, peaking on the day of maximum stigma receptivity. The nectar sugar composition differed between floral phases; it was sucrose-dominant in the male phase and sucrose-rich in the female phase. Significant year effects were observed for flowering abundance, nectar traits (volume, sugar production, concentration), and pollen output. Flowers were visited predominantly by honey bees, but bumblebees, solitary bees, and dipterans were also recorded. These results demonstrate that floral reward traits vary between years and contribute to differences in the temporal availability of nectar and pollen resources. Full article

Oat (Avena sativa L.), as an essential dual-purpose grain and forage crop, exhibits lodging resistance as a key factor directly impacting yield and quality. Therefore, breeding new oat varieties with lodging resistance is important to increase crop productivity and economic benefits. Using 130 oat germplasm as materials, 7 lodging resistance-related traits of oat, including plant height (PH), the fresh weight of single stem (FWSS), the length of basal second internode (LBSI), diameter of basal second internode (DBSI), wall thickness of basal second internode (WTBSI), stem breaking strength (SBS), and stalk puncture strength (SPS), were investigated in two experimental sites for one year. The results indicate that the seven lodging resistance-related traits exhibit a continuous distribution overall and generally follow a typical distribution pattern. A total of 36,928,068 high-quality Single-nucleotide polymorphisms (SNPs) generated from whole-genome resequencing were used for genome-wide association study (GWAS). Based on the BLINK (Bayesian-information and Linkage-disequilibrium Iteratively Nested Keyway) model threshold (−log₁₀(P) ≥ 6), 379 quantitative trait nucleotides (QTNs) associated with lodging resistance-related traits were identified. Among them, 38, 34, 78, 66, 55, 18, and 94 QTNs were associated with PH, FWSS, SBS, SPS, LBSI, DBSI, and WTBSI, respectively. Notably, three QTNs associated with FWSS and one QTN associated with SBS were stably detected across both environments, representing valuable markers for molecular breeding. From these loci, 54 candidate genes were annotated. Ranked by the number of candidate genes per trait, LBSI contained the highest number (14), followed by WTBSI (12), SPS (11), SBS (7), PH (5), and FWSS (5). Our findings provide critical support for analyzing the genetic mechanism of oat lodging resistance. Moreover, this study also offers a material and theoretical basis for the subsequent development of molecular markers and the breeding of new lodging-resistant oat varieties. Full article

In recent years, continuous cropping has become a major constraint on soybean production in China, and thus, researching methods to improve soybean yield under this cropping pattern has become a research hotspot. This study aimed to explore whether cultivar improvement and different fertilization regimes could enhance the nutrient uptake and resource utilization of continuously cropped soybean, thereby elevating its yield potential. A total of 11 soybean cultivars were subjected to different fertilization treatments, with the leaf area index (LAI), net photosynthetic rate (Pn), radiation use efficiency (RUE), dry matter accumulation, crop growth rate (CGR), nitrogen content (NC), grain yield, yield components, and harvest index (HI) analyzed. Compared with early cultivars, current cultivars increased LAI, Pn, CGR, RUE, NC, dry matter accumulation, grains per plant, 100-seed weight, HI, and grain yield by 26.22%, 10.07%, 34.13%, 22.65%, 20.43%, 29.44%, 30.09%, 9.80%, 13.69%, and 15.88%, respectively, while decreasing the nitrogen requirement per 100 g grain by 20.08%. Similarly, compared with unfertilized plants, fertilized plants increased these indices by 23.93%, 14.08%, 53.38%, 39.01%, 29.53%, 42.49%, 16.95%, 23.35%, 10.49%, and 26.50%, respectively, while decreasing the nitrogen requirement per 100 g grain by 14.40%, with the highest yield observed by the 2006-era cultivar (Dennison) fed compound fertilizer. In conclusion, cultivar improvement and fertilization can improve the yield potential of continuously cropped soybean by enhancing light energy and optimizing nitrogen accumulation and consumption, and future research should focus more on breeding to further tap the production potential of continuously cropped soybean. Full article

Contemporary agriculture faces the challenge of sustaining crop productivity amid increasing climatic pressures and simplified agronomic practices, such as monoculture. A field experiment conducted from 2022 to 2024 aimed to determine the effects of meteorological conditions and biological amendments on grain yield and yield structure in three maturity groups of continuous maize (Zea mays L.; FAO 200, 230 and 260). The split-plot experiment included applications of the biological amendments Neosol, Bactim Gleba and UGmax. Deteriorating agrometeorological conditions over the years studied led to a progressive decline in mean grain yield, reaching the lowest value in 2024 (5.06 Mg ha⁻¹). The cultivar belonging to the FAO 260 maturity group exhibited the highest yield potential. Application of all biological amendments resulted in a significant increase in grain yield and thousand-grain weight compared with the untreated control. The most effective treatment was UGmax which increased mean grain yield by approximately 14% and thousand-grain weight by 19% compared with the control. Path analysis revealed hierarchical relationships among components of ear structure and grain yield. The primary direct effect on yield increase was the number of kernels per ear, with thousand-grain weight also contributing significantly depending on maturity group. In later-maturing cultivars, kernel number per ear played the dominant role, whereas thousand-grain weight was more influential in earlier-maturing ones. The economic analysis demonstrated that all of the applied biological amendments generated a positive net profit, with the highest additional revenue obtained following the application of UGmax (160 USD·ha⁻¹). These results confirm that biostimulant application affected grain yield formation, and reduced yield losses under stress conditions. Full article