Search Results (6,868)

The high-precision, continuous monitoring of the surface water level is of great importance for water resource management and the conservation of ecological systems. This study proposes a GNSS-IR-based water-level estimation method using NMEA observations collected from low-cost GNSS receivers. First, the NMEA-recorded satellite elevation angle, azimuth angle, and signal-to-noise ratio (SNR) are processed using time-series characteristics for improving the resolution and applicability of these GNSS observations. Then, the multi-frequency GNSS signal-based reflector height inversion models are developed by making use of the Lomb–Scargle periodogram method. Finally, the Velocity Pausing Particle Swarm Optimization (VPPSO) algorithm is employed to calculate the reflector height estimation and thus the water level. Two experimental data sets collected in two different environments were used to test the proposed method. The experimental results show that the root mean square error (RMSE) of the water-level estimation error is less than 6 cm for the proposed method when the in situ ones are in the range of 196.4 cm to 296.1 cm. This study provides a theoretical and technical foundation for the development of the low-cost GNSS-IR water-level measuring instrument. Full article

Primary succession following glacier retreat provides a natural system for testing whether soil development simply shifts fine roots along a single acquisitive–conservative axis orinstead changes the nutrient-acquisition pathway that dominates at the community level. We hypothesized a stage-dependent sequence, from substrate-limited exploration, to transient morphological capture, and finally to rhizosphere-mediated biochemical mobilization. To test this idea, we quantified fine-root morphology, absorptive-transport partitioning, anatomy, phosphatase activity, exudation, community-scale belowground structure, and soil and rhizosphere properties across woody communities representing approximately 20, 40, and 90 years since deglaciation in the Hailuogou Glacier foreland. Across succession stages, bulk density and pH declined, whereas field capacity, soil carbon, and soil nitrogen increased, indicating rapid development of the belowground resource environment. Fine-root strategies did not fall along a single acquisitive–conservative continuum. Instead, morphological nutrient capture peaked at intermediate succession: the 40-year stage had the highest specific root length, specific root area, absorptive-to-transport root length ratio, and root nitrogen concentration. In contrast, the 90-year stage showed lower specific root length but higher dry matter content, thicker cortex, greater standing fine-root biomass, larger rhizosphere volume, higher phosphatase activity, and greater area-based carbon exudation. This late-successional syndrome coincided with stronger extracellular enzyme activity, larger dissolved organic carbon and nitrogen pools, and higher microbial biomass, despite negative net nitrogen mineralization. Species-level analyses showed that biochemical-input traits were jointly shaped by successional stage, species identity, and their interaction. Together, these results show that primary succession did not simply increase or decrease root acquisitiveness. Instead, as soils developed, it changed the nutrient-acquisition pathway that dominated, with direct implications for nutrient cycling and vegetation dynamics in rapidly developing glacier-foreland ecosystems. Full article

Soil erosion and nutrient loss degrade the soil resource base and water quality in dryland agricultural landscapes, yet optimal design of vegetation buffers for soil conservation under intensifying rainfall remains poorly quantified, particularly for nutrient retention. This study is novel in integrating event-scale rainfall-simulation experiments, Bayesian hierarchical modelling, Causal Forest analysis, and WEPP simulations to quantify how the sequential addition of biocrusts and grasses to shrub buffers shifts density thresholds for runoff, soil loss, and nutrient export across varying rainfall intensities. Experiments were conducted across a continuous shrub-density gradient (0–11,429 plants ha⁻¹) representing three configurations: shrub monoculture, shrub-biocrust, and shrub-biocrust-grass on agricultural hillslopes of the Chinese Loess Plateau. Runoff, soil loss, and exports of total nitrogen (TN) and total phosphorus (TP) were measured. Results demonstrate three main findings. First, multilayer shrub–biocrust–grass buffers exhibited lower soil loss than monocultures. Posterior estimates indicate reductions from approximately 3.8 t ha⁻¹ at moderate monoculture density to below 1.0 t ha⁻¹ at lower planting densities, with 94% of the highest-density intervals reflecting uncertainty in these estimates. Second, Causal Forest analysis reveals a functional separation of controls: rainfall intensity dominates soil loss (88% importance) and runoff (84%), whereas nutrient retention responds more strongly to buffer structure and density management. Third, WEPP simulations across rainfall intensities (50–180 mm h⁻¹) and slopes (10–30%) identify an optimal multilayer buffer density of 3800–5700 plants ha⁻¹, which delivers robust multifunctional benefits with 50–67% lower planting requirements than conventional high-density monocultures. These findings demonstrate that multilayer vegetation buffers enhance soil retention and reduce nitrogen and phosphorus losses from hillslopes, sustaining the soil resource base and protecting water quality in dryland agricultural landscapes. The integrated modelling framework provides transferable, evidence-based density recommendations for climate-resilient soil conservation in similar dryland regions. Full article

Carbon is an essential component in the regulation of climate systems through the global biogeochemical cycle. However, changes in land use/land cover (LULC) have reduced the capacity of terrestrial ecosystems like watershed to store carbon. This shows the need for a policy framework that balances conservative objectives with agricultural demands, as watersheds are required to support carbon storage and food production. Previous studies have generally assessed carbon dynamics or LULC change separately, with limited integration of policy-driven scenarios. Therefore, this study aimed to conduct multi-scenario carbon storage modeling to evaluate LULC protection strategies in the Cimanuk Watershed, Indonesia, an area experiencing significant LULC pressures. The method used consisted of Support Vector Machine (SVM)–Markov, the Integrated Valuation of Ecosystem Services and Trade-offs (InVEST), Geodetector, and Getis-Ord Gi*. A total of four scenarios were used to project LULC and carbon storage in 2042, which included Business as Usual (BAU), Paddy Field Protection (PFP), Forest Protection (FOP), and Paddy Field and Forest Protection (PFFOP). The results showed that forest area declined by 39,400 ha between 2015 and 2025, thereby reducing carbon storage. The PFFOP scenario was identified as the most viable, combining the protection of paddy fields and forests to balance agricultural production and carbon sequestration. Among the factors analyzed, slope exerted the greatest influence on carbon storage. Spatial cluster analysis showed that carbon hotspots were predominantly located in the upper Cimanuk sub-watershed. These results offered valuable insights into scenario-based sustainable watershed management to optimize carbon storage and maintain agricultural function. Furthermore, the proposed framework showed promising potential for application in other tropical watersheds, serving as a reference for decision-makers in sustainable watershed management. Full article

Copy number variations (CNVs) are a major source of structural genomic diversity that influence adaptation, reproduction, and production traits in livestock. The Black Bengal goat, an economically important Indian breed known for its high fecundity, superior skin quality, and resilience to humid tropical climates, was studied to uncover its structural genomic landscape. We performed whole-genome CNV analysis using high-depth (10×) sequencing data from eight individuals. A total of 31,816 copy number variants (CNVs) were identified, predominantly duplications, with an average length of approximately 45 kb. These CNVs were combined into 8910 copy number variation regions (CNVRs) covering approximately 0.15 Gb (about 5.3% of the autosomal genome). CNVR hotspots were mainly located on chromosomes 1. Gene annotation showed that regions overlapping with CNVs and CNVRs contained over 1987 protein-coding genes that are involved in pathways related to immunity, reproduction, metabolism, and extracellular matrix (ECM) organization. The presence of copy number variations involving genes such as GDF9 and BMPR1B on chromosome 7 & 6, respectively, are important because it indicates that the breed has a high reproductive capacity due to dosage-sensitive duplications. Changes in the extracellular matrix and increased dermal strength have been linked to duplications of genes such as COL6A1, LAMC2, LAMB3, FMN1, and CLDN1. This helps explain the superior hide quality of the breed. This research offers a comprehensive map of CNVs and CNVRs within the genome of the Black Bengal goat. It demonstrates how these duplications lead to structural changes that enhance both reproductive performance and skin resilience. These findings provide a valuable genomic resource for future marker-assisted selection, comparative genomics, and conservation breeding programs aimed at preserving indigenous goat populations. Full article

Wild edible fruits play an important role in supporting food security, nutrition, and traditional knowledge systems in rural communities, yet their diversity and uses remain insufficiently documented in many parts of Thailand. This study aimed to investigate the diversity, utilization, and ethnobotanical significance of wild edible fruit species in Kut Chum District, Yasothon Province. Ethnobotanical data were collected through semi-structured interviews and field surveys with local informants, and quantitative indices, including the Cultural Importance Index (CI), Fidelity Level (%FL), and Informant Consensus Factor (ICF), were applied to evaluate species significance and medicinal agreement. A total of 71 species belonging to 33 families were recorded, with most species consumed as fresh fruits and a subset used for medicinal purposes. Several species, such as Irvingia malayana Oliv. ex A.W.Benn., Phyllanthus emblica L., and Syzygium cumini (L.) Skeels exhibited high cultural importance, reflecting their key roles in local diets. High ICF values across therapeutic categories indicated strong consensus in ethnomedicinal knowledge. Additionally, 44 species not used medicinally in the study area were reported as medicinal in other regions, highlighting spatial variation in knowledge systems. These findings emphasize the importance of wild edible fruits as multifunctional resources contributing to food and nutritional security. Integrating culturally important species into conservation and sustainable use strategies may support biodiversity preservation and the continuity of traditional ecological knowledge. Full article

Wild relatives of cultivated legumes represent valuable genetic resources for crop improvement, ecosystem resilience, and sustainable agriculture. This study presents a comparative ecological and metagenomic assessment of three biogeographically distinct regions in Bulgaria—Kaliakra, Strandzha, and the Eastern Rhodopes—where populations of wild legumes, including Pisum elatius, Cicer montbrettii, Vicia incisa, and Lupinus spp., occur. Field expeditions were conducted during flowering and seed maturation stages, followed by rhizosphere soil sampling and high-throughput sequencing targeting bacterial 16S rRNA and fungal ITS regions. Soil physicochemical properties, microbial diversity indices, and taxonomic composition were analysed and compared among regions. Distinct microbial community structures were identified. Kaliakra soils were dominated by Firmicutes (26–58%) and Proteobacteria (20–25%), while Strandzha soils showed higher abundance of Actinobacteriota (12–68%) and Proteobacteria (10–35%). The Eastern Rhodopes exhibited more balanced communities, with Proteobacteria (30–45%), Firmicutes (7–43%), and Actinobacteriota (3–11%). Fungal communities also differed significantly, with Nectriaceae dominating in Kaliakra, higher evenness in Strandzha, and intermediate diversity in the Eastern Rhodopes. Alpha diversity revealed the highest bacterial richness in Kaliakra, whereas the Eastern Rhodopes showed the greatest community evenness. Beta diversity analysis demonstrated clear regional separation driven by environmental filtering. These findings highlight the ecological and microbiological differentiation of wild legume habitats and support their conservation and utilisation in sustainable agriculture and breeding programs. Full article

Sustainable groundwater management represents a main goal for the future in the context of climate change and increasing anthropogenic pressure. In recent decades, intrinsic vulnerability assessment and risk mapping have been established as some of the most important tools for groundwater preservation, but they have also shown limitations due to their static nature and their failure to account for the inherent uncertainty of hydrogeological parameters. This study proposes an innovative hybrid framework that integrates traditional overlay-index methodology (SINTACS Release 5) with stochastic numerical modeling to assess groundwater contamination risk and evolve it into a dynamic time-dependent tool. This methodology was applied to a case study of the Lapisina Valley phreatic aquifer (Northeastern Italy), a strategic area for drinking water supply. Numerical simulations were implemented to reproduce groundwater flow using the MODFLOW-NWT code. To address parametric uncertainty, 237 stochastic realizations of the modeling domain were generated using the Latin Hypercube Sampling method, randomizing hydraulic conductivity values. Advective transport was simulated through forward particle tracking using the MODPATH code, starting from the identified and classified hazard sources within the study area. Assuming the absence of attenuation during transport allowed for a conservative worst-case scenario. The result was the definition of a probabilistic contaminant propagation factor, a time-dependent indicator that quantifies the probability of pollution arrival to a specific discrete portion of the domain. This probabilistic factor was combined with three indexes commonly utilized for risk assessment (the intrinsic vulnerability index, hazard index, and value of the resource) to generate four contamination risk maps representing different timestep scenarios (5, 10, 20, and 50 years) after the arrival of a hypothetical contaminant in the saturated zone. This approach transforms risk mapping from being a useful but static snapshot to a predictive dynamic framework. Full article

The coordinated advancement of economic growth, ecological resource constraints, and societal well-being is pivotal to achieving high-quality development in the Yellow River Basin. The influence of the digital economy on urban development manifests as systemic, dynamic, and spatially heterogeneous—exhibiting pronounced interurban correlations. This study adopts a dual analytical lens: first, ecological well-being performance—which quantifies the efficiency with which natural resource inputs are converted into improvements in residents’ subjective and objective well-being; and second, ecological common prosperity—which captures equity dimensions in the distribution of ecological benefits across populations and territories. Drawing on system dynamics modeling, we elucidate the underlying mechanisms through which the digital economy shapes ecological well-being performance, simulate evolutionary trajectories of both ecological well-being performance and ecological common prosperity under alternative policy regimes, and identify optimal intervention pathways. Empirical results indicate that the integrated policy scenario combining green welfare enhancement and economic growth-oriented development, with digitally driven and regional cooperation, yields the greatest improvement in ecological well-being performance; the green welfare–focused scenario ranks second. Grounded in regional heterogeneity, we recommend a phased, differentiated implementation strategy—tailored to the developmental stage, endowments, and institutional capacities of individual cities within the basin. Methodologically, this study advances beyond conventional city-centric frameworks by introducing a local–neighborhood coupled simulation model that explicitly accounts for intercity spillovers. Furthermore, our measurement of ecological well-being performance integrates text-based big data analytics—leveraging sentiment lexicons—to robustly capture subjective welfare dimensions, thereby addressing a longstanding limitation in extant literature regarding the operationalization of well-being. Collectively, these contributions provide theoretically grounded, empirically informed insights to support the strategic implementation of ecological conservation and high-quality development in the Yellow River Basin. Full article

The vineyards of El-Kantara (Calceus Herculis, Algeria) have an ancient viticultural tradition that has never been scientifically documented. This study aims to evaluate the genetic and phenotypic diversity of this region to preserve native grapevine genetic resources. A combined approach of simple sequence repeat (SSR) markers and ampelographic characterization based on 35 Organisation Internationale de la Vigne et du Vin (OIV) descriptors was applied to 51 grapevine cultivars. Genetic analysis revealed moderate diversity, identifying eight international Mediterranean varieties, four known Algerian cultivars, and six novel genotypes with proposed names ‘Aïn Taher’, ‘Ineb Ganteri’, ‘Datté Ganteri’, ‘Seouikiya’, ‘Bayedha d’El-Kantara’, and ‘Ineb ElDjebel’. Ampelographic analyses revealed significant phenotypic variation, with principal component analysis (PCA) explaining 77% of the total variance, primarily driven by vein length and sinus shape. Cluster analysis demonstrated strong alignment between molecular and morphological data, grouping the novel genotypes into distinct morphological categories. These findings highlight a unique and previously undocumented genetic heritage in El-Kantara’s vineyards and underscore the need for a national strategy to conserve and promote Algeria’s native grapevine resources, ensuring their preservation for future viticultural and breeding programmes. Full article

As China’s first World Heritage Mixed Property site, Mount Tai enjoys international renown, with its historic buildings serving both as the central carriers of its cultural heritage and as significant tourism resources. Existing studies have predominantly emphasized the form, scale, and construction techniques of individual buildings or architectural complexes, while less attention has been given to the overall spatial pattern shaped by the interplay of natural and social environments and to the mechanisms underlying its formation. Taking the administrative area of Tai’an City as the study extent, this research selects 451 officially protected historic buildings, classified by period and type, and employs GIS-based spatial analysis and statistical methods to examine their spatiotemporal distribution patterns and influencing factors. The results indicate the following. (1) The temporal distribution exhibits an И-shaped fluctuation pattern, with ancient architecture and ancient sites together accounting for nearly 60% of the total and constituting the core resource categories. This distribution curve is shaped jointly by preservation conditions, social stability, and heritage designation preferences. (2) The spatial distribution displays a pronounced clustering pattern, with the kernel density core shifting over forty kilometers from southwest to northeast, generating an evolutionary trajectory from Dawen River basin agglomeration to Mount Tai mountain belt agglomeration. (3) The overall pattern is associated with both natural and anthropogenic factors. During the early stages, natural conditions such as hydrology and topography provided foundational constraints, whereas in later periods, human factors, including fengshan ritual culture, religious activities, economic development, and institutional governance, exhibit increasingly apparent associations with the distribution pattern. Based on these findings, this study proposes a strategic spatial framework comprising one cultural pilgrimage ring and four thematic corridors, which translates the spatial analytical results into planning implications for the regional integration of historic building resources, and discusses differentiated conservation strategies, thereby providing an analytical foundation and a reference pathway for the dissemination of Mount Tai culture and the sustainable development of heritage tourism. Full article

Indigenous pig breeds represent valuable reservoirs of genetic diversity but face increasing risks of genetic erosion due to uncontrolled crossbreeding with commercial lines. The Ashanti Dwarf Pig (ADP) of Ghana is an important local genetic resource well-adapted to tropical environments but poorly characterised at the genomic level. Using high-density SNP data from the ADPs and publicly available datasets from other African, European, and Asian pig populations, we examined genetic diversity, population structure, inbreeding, and selection signatures. After quality control, 59,124 SNPs across 875 individuals were retained. ADPs exhibited high polymorphism (~99%) and moderate heterozygosity but also elevated inbreeding (FIS = 0.15; FROH = 0.40), indicating recent inbreeding under free-range management. Population structure revealed that ADPs cluster closely with other African pigs and European breeds more than Chinese breeds. ADMIXTURE analysis, however, indicated recent introgression from both European and Chinese lines. Selection scans revealed candidate genes linked to metabolism-Zinc Finger Ran-Binding Protein 3 (ZRANB3), growth-Sortilin Related VPS10 Domain Containing Receptor 1 (SORCS1), reproduction–Sus Scrofa Chromosome 9 quantitative trait loci (SSC9 QTLs), and immunity-Tudor Domain-Containing Protein 3 and CKLF-like MARVEL transmembrane Domain Containing 7 (TDRD3, CMTM7), reflecting adaptation to tropical production systems. Our results provide a comprehensive genomic characterisation of the ADP within a global context, revealing both genetic richness and vulnerability to genetic erosion. These findings underscore the importance of structured breeding and conservation strategies in preserving this unique African genetic resource and supporting sustainable pig production under changing climatic conditions. Full article

Environmental DNA (eDNA) metabarcoding has emerged as a powerful tool for biodiversity monitoring, yet its accuracy is fundamentally constrained by the completeness and taxonomic reliability of reference sequence databases. For the Three Gorges Reservoir (TGR), no integrated multi-marker eDNA reference library exists, hampering standardized fish conservation monitoring under the Yangtze River Ten-Year Fishing Ban. Here, we constructed a comprehensive, multi-marker eDNA reference database for the fish fauna of the TGR, encompassing mitochondrial 12S rRNA, 16S rRNA, and cytochrome c oxidase subunit I (COI) gene sequences from 173 specimens (120 species) collected between 2021 and 2024. After integrating publicly available sequences, the final database comprised 161 species. Then, we quantitatively compared species annotation performance between this local database and public repositories. Results showed that while public databases achieved higher nominal species coverage (94.67%), they exhibited critical deficiencies in annotation accuracy, correctly annotating only 77.97% (12S rRNA), 75.00% (16S rRNA), and 38.14% (COI) of sequences from shared species under controlled conditions. In contrast, the local database exhibited 92.37%, 93.10% and 100% annotation accuracy for the respective markers. Optimal interspecific Kimura 2-parameter (K2P) thresholds for species delimitation were 0.00448 (12S rRNA), 0.00531 (16S rRNA), and 0.00734 (COI). In addition, 15, 0, and 4 species pairs exhibited zero interspecific distance for 12S rRNA, 16S rRNA, and COI, respectively. These limitations reinforce the need for cautious interpretation of eDNA metabarcoding results and the integration of multiple markers or complementary nuclear loci. This study provides preliminary evidence that regionally curated, multi-marker reference libraries could improve taxonomic assignment reliability in eDNA metabarcoding compared to uncurated public repositories, providing a foundational resource for biodiversity conservation. Full article

Conservation agriculture (CA) is widely promoted as a climate-smart approach to improve productivity and resilience, especially among small-scale farmers who face socioeconomic and climate-related risks that threaten their livelihoods. However, evidence linking CA adoption to household food-security outcomes in South Africa remains limited. This study examines patterns and determinants of CA adoption and assesses its implications for household food security among small-scale farmers in three municipalities of Mpumalanga Province. A quantitative cross-sectional survey was conducted among 391 farmers selected through stratified random sampling. Data were collected using a structured questionnaire and analyzed using descriptive statistics, chi-square tests, Kruskal–Wallis tests, and binary logistic regression. Results show that CA adoption was widespread but largely partial, with most farmers adopting one or two principles rather than the full CA package. Access to CA-related resources and information, household size, livelihood strategy, farm income, and farm size significantly influenced adoption. Higher adoption intensity was consistently associated with improved food-security outcomes, including increased production, lower food-insecurity severity, greater crop diversification, higher likelihood of year-round production, and increased market participation. The study concludes that conservation agriculture can contribute positively to multiple dimensions of household food security when adopted as an integrated system, but partial adoption yields limited benefits. Targeted extension support, improved access to resources, and context-specific interventions are required to enhance sustained and holistic CA adoption among small-scale farmers. Full article

The transition toward a sustainable agri-food system, aligned with agricultural and environmental policy objectives, has increased interest in aromatic plants as non-synthetic pesticide alternatives. This study focused on evaluating the antifungal potential of five specific aromatic plant species, particularly Lavandula dentata, Origanum vulgare, Thymus vulgaris, Salvia officinalis and Rosmarinus officinalis, against the phytopathogenic soil-borne fungi Fusarium oxysporum f.sp. radicis-lycopersici and Verticillium dahliae. During screening, L. dentata and T. vulgaris extracts exhibited strong in vitro fungicidal activity. Bioactive compounds previously detected in both lavender and thyme were identified in their extracts using a triple quadrupole/linear ion trap mass spectrometer. Assessment of in vitro phytoprotective action of L. dentata extract in solid and liquid growth media demonstrated inhibitory effects against F. oxysporum f.sp. radicis-lycopersici at concentrations above 1% v/v, with inhibitory effects of L. dentata extract being observed at concentrations equal to or above 2% v/v. T. vulgaris extract inhibited V. dahliae growth on solid media at concentrations at 1% v/v or above, while inhibitory effects were observed in broth media containing 2% v/v thyme extract. Seed germination tests of both L. dentata and T. vulgaris revealed a concentration-dependent reduction in their germination index (GI) at concentrations equal or above 2% v/v, apart from the effect of lavender extract on cress, where inhibition occurred at dose application above 5% v/v. In planta experiments demonstrated the complete phytoprotective action of lavender extract against F. oxysporum f.sp. radicis-lycopersici, while a marginal improvement in plant survival was observed during application of T. vulgaris extract. Full article