Search Results (11,924)

The rapid proliferation of Internet of Things (IoT) devices in cloud-based e-learning platforms has posed significant security risks, particularly in protecting learner information, authentication of devices, and safe communication in the highly heterogeneous learning settings. Current cryptographic solutions are largely based on classical public-key infrastructure (PKI) protocols such as RSA and ECC, which will become vulnerable with the advent of large-scale quantum computers capable of executing Shor’s algorithm. In addition, traditional perimeter-based security models are inadequate for handling the dynamics, scattered, and resource-limited characteristics of IoT-enabled educational systems. As a solution to these problems, this paper introduces ZeroTrustEdu, a scalable zero-trust cryptographic solution that combines lightweight post-quantum key management with adaptive trust scoring of cloud-connected IoT e-learning infrastructure. The proposed framework makes three fundamental contributions namely: (1) a hierarchical zero-trust security model with no implicit trust, operating across device, edge, and cloud layers; (2) a lightweight key distribution protocol based on the Module-Lattice Key Encapsulation Mechanism (ML-KEM) compliant with NIST FIPS 203 standards and (3) an adaptive behavioral trust scoring engine that dynamically adjusts device and user trust levels based on real-time interaction analytics. The architecture is evaluated using extensive NS-3 network simulations with up to 100,000 concurrent IoT nodes with formal security analysis under Chosen Plaintext Attack (CPA) and Chosen Ciphertext Attack (CCA) threat models. Comparative evaluation against RSA-2048, ECC-P256, and AES-256 baselines demonstrates that, ZeroTrustEdu delivers a 62% ± 3% (95% CI, 10 independent runs) reduction in ML-KEM encapsulation latency (12.8 ms for key encapsulation/decapsulation, contributing to a complete device authentication latency of 47.3 ms including ML-DSA signature operations), 45% reduced communication overheads, and 38% reduction in energy consumption on ARM Cortex-M4 constrained devices compared to RSA-2048 and achieves provable post-quantum security reducible to the hardness of the Module Learning With Errors (MLWE) problem. These findings demonstrate that the proposed architecture provides a viable, scalable, and quantum-resilient security solution for next-generation IoT-enabled e-learning environments. The cryptographic security of ZeroTrustEdu is guaranteed at the primitive level through NIST-standardized ML-KEM (FIPS 203) and ML-DSA (FIPS 204), with IND-CCA2 and EUF-CMA security formally proven in the respective standards; full protocol-level formal verification using automated theorem provers (ProVerif, Tamarin) is identified as valuable future work to rule out protocol-composition vulnerabilities beyond primitive-level guarantees. Full article

Cervical cancer screening with high-risk human papillomavirus (HR-HPV) testing requires effective triage of HPV-positive women. Koilocytosis is a classic cytopathic effect of HPV infection, but its clinical significance in low-grade squamous intraepithelial lesions (LSILs) remains unclear. We retrospectively evaluated 157 HPV-positive women with LSIL cytology and follow-up data, including 140 women with concurrent biopsy results. Koilocytes were identified in 93/157 cases (59.2%) and were less frequent in HPV16/18-positive cases. Cervical intraepithelial neoplasia ≥ grade 2 (CIN2+) was detected in 9/84 koilocyte-positive cases (10.7%) and 16/56 koilocyte-negative cases (28.6%), whereas non-CIN findings were more common in koilocyte-positive cases. Koilocyte-positive cases also showed a longer time to regression from LSIL to negative for intraepithelial lesions or malignancy. These findings suggest that koilocytosis mainly reflects productive HPV infection and has limited utility for predicting CIN2+ in HPV-based screening triage. Excluding koilocytosis-driven low-grade cytological changes from triage positivity criteria may improve specificity and positive predictive value, supporting higher triage thresholds. Full article

Surgical intervention is a primary treatment for osteosarcoma, often resulting in a tumorous bone defect with an irregular shape. Postoperative management is essential to minimize tumor recurrence risks and promote bone regeneration. To address these issues, we developed a multifunctional injectable, rapidly photo-curable hydrogel composed of gelatin methacryloyl/carboxymethyl chitosan/hyaluronic acid (GelMA/CMCS/HA), modified with vanadium-doped mesoporous bioactive glass (VMBG). The exceptional injectability enables seamless adaptation to irregular bone defects, offering a significant advantage over preformed implants, while the rapid photocurability of the hydrogel ensures stable fixation within minutes, thereby reducing potential risks during surgery. Furthermore, this platform exhibits dual therapeutic efficacy, characterized by antitumor activity and osteogenic induction. In vitro assessments demonstrated that V(V)/V(IV) valence cycling-driven ROS generation mediated its potent antitumor efficacy. Additionally, concurrent enhancement of alkaline phosphatase activity and osteogenic marker expression validated its osteogenic potential. The CMCS incorporation promoted healing at the defect site, while the HA addition created binding sites for cell adhesion and growth, thereby improving scaffold bioactivity. Collectively, this study presents the development and validation of a multifunctional GelMA/CMCS/HA hydrogel, highlighting its dual capability for bone regeneration and tumor suppression within tumor-associated bone microenvironments. Full article

The solidification process is crucial for preparing high-performance superconductors and is strongly dependent on the characteristics of the starting powder, including particle size, morphology, and phase purity. This review concisely examines the study on four key superconductors: REBCO, Bi-2212, FeSeTe, and MgB₂. In REBCO, additives such as CeO₂, Pt, or BaO₂ powder can refine the RE-211 phase. In Bi-2212, Pb doping stabilizes the high-T_c phase. For FeSeTe, doping with F or Co modifies phase separation and introduces Δκ pinning. Meanwhile, in MgB₂, the incorporation of SiC nanoparticles powder generates effective pinning centers. Concurrently, processing conditions exert a decisive influence on the final microstructure, as demonstrated by the TSMG/TSIG route in REBCO, partial melting parameters for Bi-2212, specific cooling protocols and thermal treatments for FeSeTe, and optimized sintering and post-annealing processes for MgB₂. Future research directions should prioritize fundamental understanding of phase separation mechanisms during powder processing, development of multi-component doping strategies for powder modification, and advancement of scalable powder processing routes for practical conductor architectures. Full article

Against the backdrop of concurrent economic transformation, deepening digitalization, and green upgrading, this study aims to uncover the mechanisms through which the system of new quality productive forces drives high-quality economic development. Drawing on a six-element framework comprising new-quality labor, new means of production, new labor objects, new technology, production organization, and data elements, the study uses panel data from 30 Chinese provinces covering the period 2014–2023 and applies dynamic qualitative comparative analysis (dynamic QCA) to examine the relevant configurational pathways and their cross-temporal evolution. The results show that no single condition constitutes a temporally and regionally stable necessary condition for high-quality economic development. Instead, high-quality economic development is primarily realized through three pathways: a technology–organization–tool-dominated pathway, a talent–data–carrier-led pathway, and a technology–organization–carrier-compensation pathway. The explanatory power of these pathways also varies across stages. The findings indicate that high-quality economic development arises from the synergistic configuration and dynamic recombination of multiple elements of new quality productive forces. Full article

Gaseous nitrogen losses and residual soil nitrogen accumulation are primary drivers of low nitrogen use efficiency in alkaline irrigated cropping systems. A two-year field experiment (2019–2020) in the Hetao Irrigation District under alkaline flood-irrigated maize evaluated the effects of nitrogen rate, fertilizer formulation, and enhanced-efficiency fertilizers—urea with urease inhibitor NBPT and ammonium sulfate with nitrification inhibitor DMPP—on NH₃ volatilization, N₂O emissions, post-harvest soil mineral nitrogen, and grain yield. A soil pH manipulation sub-experiment (±0.5 units, ambient pH ~8.8) was conducted to quantify the direct effect of alkalinity on volatilization. NH₃ volatilization was insensitive to fertilizer formulation and inhibitor inclusion but strongly responsive to soil pH; a 0.5-unit increase in soil pH elevated volatilization efficiency by up to 25% relative to ambient conditions. N₂O emissions were around 18% higher under ammonium sulfate than under urea and were reduced by 21–32% with inhibitor treatments, without increasing NH₃ volatilization. Inhibitor-assisted optimized management (urea + NBPT and ammonium sulfate + DMPP) achieved higher yields and lower emission intensity than urea alone. These results confirm that NH₃ and N₂O losses are governed by distinct controls, and that concurrent mitigation of both pathways requires interventions that independently target each loss driver, beyond rate optimization and inhibitor application alone. Full article

Bacillus velezensis-based probiotics are increasingly recognized for their potential to enhance intestinal health in companion animals, yet their mechanisms of action in canine epithelial systems remain incompletely defined. This study aimed to evaluate whether a live Bacillus velezensis probiotic consortia (BC) modulates epithelial barrier integrity, immune signaling, apoptosis-renewal pathways, and metabolic activity in canine-relevant intestinal and macrophage cell models. MCA-B1 proximal gastrointestinal epithelial cells and DH82 macrophage-like cells were exposed to BC cultures, followed by quantification of tight-junction expression, permeability (FITC-Dextran), cytokine responses, phagocytic activity, apoptosis-related markers, and metabolomic profiles. BC treatment significantly strengthened the epithelial barrier, inducing a marked upregulation of Claudin 1 (CLDN1) (11.3 fold), CLDN4 (2.4 fold), Occludin (OCLN, 1.7 fold), and increasing key proteins including ZO-2 and cingulin while reducing LPS-induced FITC-Dextran permeability to 94.5%. BC concurrently modulated innate immune signaling, increasing MyD88 (33.2%), IL-8 (14.6 fold), IL-18 (2.6 fold), and IFNB1 protein levels, while enhancing anti-inflammatory regulation, including a robust rise in DH82-derived IL-10. Apoptosis-renewal markers shifted toward physiological turnover, with increased BCL2 (1.9 fold) and reduced BAK1. Metabolomic profiling of BC activity revealed elevated AMP, abundant Peptide Transporter 1 (PEPT1)-transportable peptides, increased γ-glutamyl metabolites, and lower Glutathione disulfide (GSSG), consistent with AMPK-linked tight-junction assembly and glutathione-supported redox buffering. Together, these data indicate that Bacillus velezensis-derived metabolites positively influence barrier-related, immunological, and metabolic responses in a canine proximal intestinal epithelial system and modulate functional responses in macrophage-like cells. These in vitro findings contribute to the mechanistic understanding of host cellular responses to Bacillus-associated metabolites. Full article

Toxoplasma gondii (T. gondi) is a widespread zoonotic parasite that poses a significant threat to global public health, yet effective therapeutic options remain limited. In this study, we found that the flavonoid compound myricetin (MYR) can significantly inhibit the proliferation of T. gondii. This effect is associated with the inhibition of dihydroorotase (TgDHO) activity in the de novo pyrimidine biosynthesis pathway, and this inhibition can be partially reversed by exogenous supplementation with uracil. Further studies revealed that MYR treatment can induce cell cycle arrest in tachyzoites and impair bradyzoite proliferation, concurrently disrupting the UDP-GlcNAc glycosylation of the cyst wall. In mouse models, MYR demonstrated significant efficacy, achieving an 80% survival rate in acute infection and inducing morphological abnormalities in intracerebral cysts during chronic infection. Collectively, these findings elucidate the anti-Toxoplasma activity and multifaceted mechanisms of MYR, providing valuable insights for developing novel therapeutics against toxoplasmosis. Full article

In our preliminary work, a clam sauce prepared by fermentation with Aspergillus oryzae 3.042 (AO) exhibited desirable flavor and quality; however, the process was prolonged (exceeding 30 d), and a high salt concentration (6–15%) was necessary to prevent spoilage. Consequently, shortening production cycle and reducing salt content without compromising product quality became a new objective. Enzymatic hydrolysis has long been recognized as an efficient approach in seasoning production, with enzyme efficacy being a key competitive factor. Accordingly, an AO-derived aminopeptidase–protease complex (AOAP) was optimized and prepared as a preparatory step. In this study, AOAP was applied to hydrolyze clam meat to evaluate its potential for producing a seasoning base. A two-step enzymatic hydrolysis process was employed. In the first step, the highest hydrolysis degree (29.1%) was achieved using alkaline protease (AP). The resulting hydrolysate was subsequently subjected to secondary hydrolysis with AOAP, achieving a degree of hydrolysis as high as 49.8%. Sensory evaluation revealed a significant reduction in bitterness and enhancement of umami in the final hydrolysate, a finding corroborated by electronic tongue analysis. Further characterization via LC-MS and amino acid (aa) analysis showed that a substantial number of bitter and umami peptides were released following AP treatment; however, the number of these peptides was markedly reduced after a subsequent AOAP hydrolysis, with concurrent substantial changes in the peptide profile. In the two-step hydrolysate, umami peptides mostly contain 3–10 aa, whereas bitter peptides typically contain only 3–5 aa. The content of free aa increased from 369.17 mg/100 g in the control to 3026.25 mg/100 g in the two-step hydrolysate, half of which were bitter, indicating the debittering efficiency of AOAP. Electronic nose analysis revealed similar flavor profile and characteristic presence of nitrogen oxides in all hydrolysates. GC-MS analysis further demonstrated that, after combined enzymatic hydrolysis, the short-chain aldehydes and ketones responsible for the fishy odor in the raw material almost completely disappeared, while long-chain aldehydes with pleasant aromas were generated. These findings suggest that the secondary hydrolysis step using AOAP can effectively improve the overall flavor profile of the clam hydrolysate, which may support its potential applicability in seasoning production, though further optimization and scale-up validation are needed. Full article

Quercus baronii Skan (Q. baronii) is an ecologically important tree species in arid and soil erosion-prone areas of northern China, and also holds significant potential as a bioenergy tree species, providing substantial ecological benefits. Global climate change has profoundly influenced the suitable habitats and habitat fragmentation of Quercus baronii forests. This study employed the Maximum Entropy (MaxEnt) model to project the current and future suitable habitats of Q. baronii forests, along with their trends of contraction and expansion. Concurrently, composite landscape indices were used to assess the fragmentation of these suitable habitats. The results indicate that the suitable habitats for Q. baronii forests are primarily located in the eastern part of Northwest China, the northern part of Central China, and the southern part of North China. Minimum temperature of the coldest month (bio6), annual precipitation (bio12), and temperature seasonality (bio4) emerged as the primary determinants of habitat suitability. Under three future climate scenarios, the centroid of suitable habitats for Q. baronii forests is projected to shift towards higher latitudes in the northwest, with the elevation of suitable habitats also gradually rising in tandem with increased carbon emissions. Under low carbon emission scenarios, the extent of suitable habitat for Q. baronii forests is expected to expand; under medium and high carbon emission scenarios, it is expected to first increase and then decline. Although over two-thirds of the suitable habitat for Q. baronii forests is projected to remain relatively intact, future suitable habitats are expected to be more fragmented compared to the present. This fragmentation is projected to intensify with increasing carbon emissions, primarily occurring at the edges of the suitable areas. The results of this study lay the groundwork for both the preservation of forest biodiversity and the ecological conservation and sustainable management of temperate broad-leaved forest ecosystems. Full article

Passive thermoregulatory textiles, operating without external energy input, play a crucial role in maintaining the human body within the thermal comfort zone. However, integrating autonomous environmental adaptability with superior wearing comfort into a single textile remains a challenge. In this work, inspired by the autonomous actuation of water lilies, we proposed an intelligent strategy to fabricate thermoregulatory textiles that dynamically adapted to ambient temperature fluctuations, driven by a bidirectional shape memory polymer (SMP). To concurrently achieve robust thermal adaptability and human-body-compatible softness, a crosslinked polyethylene glycol–butyl acrylate (PEG-BA) bidirectional SMP network was engineered. The PEG phase, featuring a broad crystal size distribution, provided the dynamic skeleton for thermally induced actuation, while the incorporation of the BA component tuned the intrinsic softness to match conventional soft textiles. Consequently, the synthesized PEG-BA network exhibited an exceptional bidirectional shape memory effect with a reversible strain of 15.5%, while maintaining high macroscopic softness comparable to that of human skin. By integrating this bidirectional polymer into a garment to form adaptive vents, the smart textile demonstrated the capability to significantly elevate human thermal comfort. Specifically, the vents autonomously open in hot environments to accelerate heat dissipation and close in cool environments to suppress heat loss. Given its exceptional personal thermoregulatory performance and wearing compliance, this proposed strategy exhibits considerable potential for maintaining optimal human comfort against fluctuating environmental conditions. Full article

In weak receiving-end grids, the active support of the valve-side static var compensator and filter (SVF) extends the commutation failure (CF) boundary of LCC-HVDC. However, SVF control state transitions reshape valve-side voltage and harmonic characteristics, causing conventional fixed threshold protection to exhibit concurrent misblocking and failure to operate risks, while SVF zone internal faults are prone to excessive pole-level escalation. This paper proposes a state-aware coordinated protection strategy for symmetric single-pole SLCC-HVDC systems. A normalized commutation margin index, derived from the commutation voltage time integral, characterizes the nonlinear CF boundary under SVF support. SVF control mode, health status, and reactive power margin serve as conditioning variables for adaptive threshold and time window modification. A three-level escalation strategy—local isolation, derated ride-through, and pole-level action—is further designed for SVF zone faults. Validation via RTDS sequence of events records and EMT–protection logic replay co-simulation shows that the proposed index achieves a 100% CF risk prediction rate across five fault scenarios, versus 40% for conventional indices. The method maintains zero failure to operate with a misblocking rate ≤ 10.1% at SNR ≥ 30 dB. The staged response correctly escalates all four SVF zone fault types to the required level, compared with two of four for the fixed threshold baseline. These results confirm effective enhancement of protection robustness, fault ride-through capability, and operational continuity for SLCC-HVDC in weak receiving-end grids. Full article

Intensive pesticide application sustains global agriculture but poses poorly characterized risks to complex soil ecosystems. Here, we quantitatively evaluated pesticide residues and utilized high-resolution environmental DNA (eDNA) metagenomics to decode multi-trophic community responses across a typical major grain-producing region located in China. Among 39 targeted pesticides, 26 were detected with total concentrations ranging from 27.9 to 478.8 ng/g. While herbicides and fungicides dominated the residual mass, insecticides posed the most severe ecological threat. Notably, the neonicotinoid imidacloprid exhibited high-risk levels (RQ = 1.78 ± 1.49) at >61.1% of the sampling sites. eDNA profiling and Procrustes analyses revealed a clear trophic-dependent sensitivity gradient (p < 0.01). Lower-trophic microbial communities were significantly altered in composition; pesticide stress was strongly associated with profound non-target suppression on keystone plant-beneficial bacteria (e.g., Nocardioides). Concurrently, the fungal eDNA profiles indicated that the soil mycobiome harbored an alarming 34.7% of potential phytopathogenic fungi (e.g., Aspergillus and Colletotrichum), intrinsically driving the massive fungicide reliance. In contrast, higher-trophic soil metazoa (Rotifera, 40.4%) and weed communities (e.g., Digitaria sanguinalis) exhibited significant spatial stability, reflecting robust environmental buffering and herbicide-driven ecological escapes. Furthermore, co-occurrence networks decoupled target from non-target toxicities, uniquely revealing that persistent herbicide metabolites (desethylatrazine) induce prolonged legacy toxicities on specific soil fauna. Collectively, this study unveils the deep, cross-kingdom ecological disruptions caused by current pesticide regimes, underscoring the urgency of integrating eDNA biomonitoring to guide precision pest management and safeguard soil health in vital agricultural hubs. Full article

Industrial control systems (ICSs) increasingly rely on legacy communication protocols such as ModbusTCP, which lack built-in security mechanisms and remain widely exposed to network-based attacks. This paper investigates the security limitations of authentication mechanisms in ModbusTCP-enabled programmable logic controllers (PLCs) and demonstrates how plaintext credential transmission and limited connection handling capabilities can be exploited to perform brute-force and denial-of-service (DoS) attacks. An experimental testbed based on two industrial Delta PLC families (DVP-13SE and DVP-311SV3) was developed to systematically evaluate these vulnerabilities under realistic conditions. The results show that authentication credentials can be easily captured through network sniffing, while the PLC communication stack supports a maximum of 16 concurrent connections and can process up to approximately 8600 Modbus operations per second, making it susceptible to resource exhaustion and performance degradation under distributed attack scenarios. To address these limitations, this paper proposes a lightweight deception-based protection mechanism, termed the PLC misleading algorithm (PMA), which is implemented directly within the PLC ladder logic. Unlike traditional network-level defenses, PMA operates at the device level and dynamically misleads attackers by generating controlled randomized responses while preserving consistent behavior for legitimate clients. Experimental results demonstrate that PMA significantly mitigates brute-force effectiveness by preventing reliable password extraction while introducing minimal overhead (2.2% memory usage) and maintaining acceptable communication latency. Additionally, the proposed approach significantly reduces observable attack traffic, with only 0.246 Modbus operations per second observed during the attack phase, thereby limiting the effectiveness of automated exploitation tools. These findings highlight the potential of in-device deception mechanisms as a practical and deployable security layer for legacy industrial systems, and provide new insights into the resilience of PLC-based infrastructures against network-level attacks. This work bridges the gap between lightweight PLC-level protections and the growing need for robust cybersecurity mechanisms in industrial IoT environments. Full article

This study comparatively investigates the efficacy of two natural, plant- and microbe-derived polysaccharides—xanthan gum (XG) and guar gum (GG)—in enhancing the water stability and shear strength of granite residual soil (GRS). GRS specimens treated with varying dosages of XG and GG were cured for 14 days and subsequently evaluated through direct shear and static-water disintegration tests. Concurrently, scanning electron microscopy (SEM) and low-field nuclear magnetic resonance (LF-NMR) were employed to elucidate the underlying microstructural and pore-scale mechanisms. Direct shear test results indicate that the peak shear strength reached 295.9 kPa (2.0% GG) and 221.0 kPa (1.5% XG), representing increases of 58.2% and 35.7%, respectively. Quantitatively, GG and XG treatments yielded maximum internal friction angle improvements of 52.96% and 39.37%, with peak cohesion increases of 55.27% and 35.7%, respectively. During static-water immersion, the untreated GRS suffered complete disintegration within 200 s. In contrast, the 2.0% GG- and XG-treated specimens preserved overall structural integrity for 24 h. SEM observations revealed that XG and GG reconstruct the soil fabric by forming encapsulating films and interparticle bridging structures. Finally, LF-NMR analysis provided definitive quantitative proof of a “pore refinement” effect, where biopolymer treatment shifted the primary $T_2$ peaks from 4.64 ms to 3.51 ms. Notably, at a 2.0% dosage, dramatic NMR signal surges (up to 747.5 a.u. for XG and 704.3 a.u. for GG) revealed that excessive biopolymers tend to form localized ‘gel lumps’ rather than uniform films. These blobs weaken the biting force between soil particles, thereby accounting for the observed degradation in shear strength. Full article