Search Results (254)

Natural hydrogen is generated by fairly deep processes and/or in low-permeability rocks. In such contexts, fluids circulate mainly through the network of faults and fractures. However, hydrogen flows from these hydrogen-generating layers can reach sedimentary rocks with more typical permeability and porosity, allowing H₂ flows to spread out rather than be concentrated in fractures. In that case, three different H₂ transport modes exist: advection (displacement of water carrying dissolved gas), diffusion, and free gas Darcy flow. Numerical models have been run to compare the efficiency of these different modes and the pathway they imply for the H₂ in a sedimentary basin with active aquifers. The results show the key roles of these aquifers but also the competition between free gas flow and the dissolved gas displacement which can go in opposite directions. Even with a conservative hypothesis on the H₂ charge, a gaseous phase exists at few kilometers deep as well as free gas accumulation. Gaseous phase displacement could be the faster and diffusion is neglectable. The modeling also allows us to predict where H₂ is expected in the soil: in fault zones, eventually above accumulations, and, more likely, due to exsolution, above shallow aquifers. Full article

Incorporation of lanthanide (Ln) and actinide (An) ions into the human body poses significant chemotoxic and radiotoxic risks, necessitating effective decorporation strategies. This study investigates the displacement of biologically relevant ligands from trivalent ions of europium, Eu(III), and curium, Cm(III), in artificial biofluids by various complexing agents, i.e., ethylenediaminetetraacetic acid (EDTA), ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA), diethylenetriaminepentaacetic acid (DTPA), and spermine-based hydroxypyridonate chelator 3,4,3-LI(1,2-HOPO) (HOPO). Utilizing a modified unified bioaccessibility method (UBM) to simulate gastrointestinal conditions, we conducted concentration-dependent displacement experiments at both room and body temperatures. Time-resolved laser-induced fluorescence spectroscopy (TRLFS) supported by ²H nuclear magnetic resonance (NMR) spectroscopy and thermodynamic modelling revealed the complexation efficacy of the agents under physiological conditions. Results demonstrate that high affinity, governed by complex stability constants and ligand pK_a values, is critical to overcome cation and anion competition and leads to effective decorporation. Additionally, there is evidence that cyclic ligands are inferior to linear ligands for this application. HOPO and DTPA exhibited superior displacement efficacy, particularly in the complete gastrointestinal tract simulation. This study highlights the utility of in vitro workflows for evaluating decorporation agents and emphasizes the need for ligands with optimal binding characteristics for enhanced chelation therapies. Full article

Peste des petits ruminants virus (PPRV) has been classified into four lineages based on the nucleocapsid and fusion genes, with lineage IV strains being the most widely distributed. In Africa, recent epidemiological data revealed that PPRV lineage IV is increasingly displacing other lineages in prevalence, suggesting a competitive advantage in viral transmission and adaptability. Moreover, a lineage IV strain was the only confirmed strain in Europe and Asia. In this study, a one-step Taqman quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) assay for lineage IV PPRV was established by targeting the hemagglutinin (H) gene. The results indicated that this method could detect approximately six copies of PPRV RNA, indicating high sensitivity. No cross-reactions with related viruses or other lineages of PPRV were observed. The results of a repeatability test indicated that the coefficient of variation values were low in both the inter-assay and intra-assay experimental groups. Detection of field samples indicated that all positive samples could be detected successfully using the developed method. This RT-qPCR assay provides a valuable tool to facilitate targeted surveillance and rapid differential diagnosis in regions with active circulation of PPRV lineage IV, enabling timely epidemiological investigations and strain-specific identification. Full article

Niacinamide was recently shown to directly interact with bacterial DNA and interfere with cell replication; niacinamide mode of interaction and efficacy as a natural anti-microbial molecule were also described. The aim of this study is to elucidate the exact binding mechanism of niacinamide to microbial DNA. Intercalation is a binding mode where a small planar molecule, such as niacinamide, is inserted between base pairs, causing structural changes in the DNA. Melting curve analysis with various intercalating dyes demonstrated that niacinamide interaction with bacterial DNA reduces its melting temperature in a linear dose-dependent manner. Niacinamide’s effect on the melting temperature was found to be % GC-dependent, while purine stretches were also found to influence the binding kinetics. Finally, fluorescent intercalator displacement (FID) assays demonstrated that niacinamide strongly reduces SYBR Safe signal in a dose-dependent manner. Interestingly, competition assays with a minor groove binder also reduced Hoechst signal but in a non-linear manner, which can be attributed to strand lengthening and unwinding following niacinamide intercalation. Taken altogether; our results suggest a “disruptive intercalation” as the mode of interaction of niacinamide with bacterial DNA. Formation of locally destabilized DNA portions by niacinamide might interfere with protein–DNA interaction and potentially affect several crucial bacterial cellular processes, e.g., DNA repair and replication, subsequently leading to cell death. Full article

Markerless motion capture (MMC) offers a non-invasive method for monitoring external load in sports where wearable devices are restricted; however, its validity in 3x3 basketball contexts remains unverified. The viability and measurement precision of a multi-camera RTMPose-based MMC system for single-player tasks in 3x3 basketball performance monitoring were evaluated in this study. Recorded on a standard half-court, eight cameras (60 fps) captured ten collegiate athletes executing basketball-specific activities including linear sprints, curved runs, T-tests, and vertical jumps. The 3D coordinates of hip and ankle keypoints were reconstructed from multiple synchronized camera views using Direct Linear Transformation (DLT), from which horizontal displacement and average speed were derived. These values were validated using tape-measure distance and time–motion analysis. The MMC system demonstrated high accuracy, with coefficients of variation (CVs) below 5%, mean bias under 3.5%, and standard error of estimate (SEE) below 3% across most tasks. Speed estimates revealed great consistency with time–motion analysis (ICC = 0.97–1.00; standardized change in mean [SCM] varied from trivial to small). The Bland–Altman graphs verified no proportional error and little bias. These results confirm the MMC system as a consistent, non-invasive method for gathering movement data in outdoor basketball environments. Future studies should assess the system’s performance during live competitive play with several athletes and occlusions and compare it to a laboratory-grade motion capture system. Full article

Interspecific interactions among sympatric carnivores are critical for understanding patterns of coexistence, competition, and community structure. Among mesocarnivores, dominance hierarchies are typically shaped by differences in body size, social organization, and competitive ability. The golden jackal (Canis aureus) is generally assumed to dominate the red fox (Vulpes vulpes) across shared landscapes, particularly at high-value resources such as carcasses. However, here, we present rare behavioral evidence that challenges this prevailing assumption. Using motion-triggered camera traps deployed at a carcass in Lake Kerkini National Park, Greece, we recorded a sequence of interactions in which a golden jackal displayed clear submissive behavior toward a red fox, including lowered body posture, tail tucking, and conflict avoidance. Subsequent footage revealed two additional agonistic encounters, during which the same red fox successfully displaced two separate jackals, one of which emitted a distress vocalization while retreating. These findings represent the first documented case of interspecific submission by golden jackals toward a red fox and suggest that context-specific factors—such as immediate carcass possession, individual experience, or body condition—may modulate expected dominance outcomes. Our observations underscore the importance of fine-scale behavioral studies in revealing plasticity in interspecific relationships and contribute to a more nuanced understanding of carnivore competition under semi-natural conditions. Full article

Toxic and non-toxic strains of Raphidiopsis raciborskii coexist widely in natural water bodies, with the dominance of toxic strains directly influencing bloom toxicity. This study investigates how temperature affects the relative dominance of toxic R. raciborskii strains and the production of cylindrospermopsin (CYN). We conducted monoculture and co-culture experiments in nutrient-rich BG11 medium at three temperatures (16 °C, 24 °C, and 32 °C) using two pairs of strains (CS506/CS510 from Australia and QDH7/N8 from China). The results revealed that the Australian strains failed to grow at 16 °C, while the Chinese strains thrived. In a co-culture experiment, the Australian toxic strain CS506 exhibited the fastest growth at 24 °C, whereas the Chinese toxic strain QDH7 reached similar maximum cell densities across all temperatures but peaked more quickly at 24 °C and 32 °C compared to 16 °C. Regardless of temperature and strain pairs, toxic strains consistently maintained a higher relative abundance than their non-toxic counterparts. Analysis using the rate of competitive displacement (RCD) model indicated that higher temperatures accelerated the displacement of non-toxic strains by toxic ones. Total CYN concentrations in co-cultures increased with temperature, although the cell quota of CYN (Q_CYN) did not vary significantly across temperatures. In co-culture, the CYN production rate during the exponential phase was positively correlated with cell growth rate, but this correlation weakened or reversed in the stationary phase, likely due to changes in nutrient availability. These findings suggest that rising temperatures under eutrophic conditions may enhance the growth and competitive advantage of toxic R. raciborskii strains, thereby exacerbating bloom toxicity. Full article

Paddler athletes use resistance training (RT) to optimize power output (PO) during competitions. Understanding the power–load relationship (P–Lr) is essential for effective RT prescription. Moreover, the push-to-pull ratio (PU/PR)—the one-repetition maximum (1RM) of a pulling exercise divided by the one of a pushing exercise—has been suggested as a metric associated with sprint kayak performance. This study aimed to describe P–Lr in three guided exercises (bench press (BP), ballistic bench press (BBP), and prone bench pull (PBP)), along with PU/PR in international-level canoeing and kayaking athletes. Nine male athletes (21.0 ± 1.5 years) were monitored during two sessions of an incremental testing protocol. Load ranged from 30 to 100 kg in BP, 30 to 95 kg in PBP, and 20 to 60 kg in BBP. Instantaneous displacement was measured using a linear position transducer, and PO was computed for each repetition and exercise. PU/PR was calculated upon PBP and BP. A two-way repeated-measures ANOVA was used to explore differences among exercises and relative load from 20% to 90% 1RM. PBP displayed a higher PO between 40% and 90% 1RM compared to BP and BBP), while no statistical difference was found between BP and BBP at any relative load. Additionally, mean PU/PR resulted 0.96. This study provides preliminary values regarding P–Lr and PU/PR in elite paddlers, which may assist in designing training programs for those targeting major competitions. Full article

Conservation of ecologically important freshwater mussels is high on the international agenda, but there is only limited knowledge about the status of rare unionid species in arid and semi-arid areas which are particularly vulnerable. One such example concerns Anodonta vescoiana which was recognized as one of the few endemic species of unionid mussels from Iraq and was restricted to the marshes of southern Mesopotamia and its connected river systems. The last confirmed report of A. vescoiana was in 2009 from the Al-Ezz River. We conducted extensive field surveys during the years 2021 and 2022 at approximately 20 freshwater sites, but we failed to observe any live or dead specimens, suggesting a probable extirpation or severe decline. In contrast, we documented the invasive Sinanodonta woodiana at numerous sites across the Tigris–Euphrates basin including the Al-Ezz River. This documentation of S. woodiana indicates successful establishment of the species and colonization of freshwater systems modified by anthropogenic practices, which include alterations of hydrological dynamics and ecological conditions. Here, we compile existing evidence of the global ecological impacts and development of S. woodiana invasion, while also highlighting Iraq as an important example of the displacement of native unionid mussel species by invasive alien unionids. We assessed the factors that contributed to the disappearance of A. vescoiana in Iraq including biological competition (with S. woodiana), salinity stress, habitat fragmentation, and pollution. The time window to act and prevent the further decline of rare unionid species in Iraq, as well as other arid and semi-arid areas which face similar threats, is short. Urgent actions include systematic monitoring to identify remnant populations, implementing biosecurity policies (for fisheries or habitats), and restoration (of habitats) to secure the long-term persistence of remaining unionid diversity. Full article

This study investigates the transfer of rural land contracting rights in Shandong Province, China, employing a mixed methods approach that integrates qualitative policy analysis and quantitative multiple linear regression models based on panel data from 16 prefecture-level cities (collected in 2010–2022). The findings demonstrate that land transfer optimizes resource allocation, enhances agricultural output efficiency, and serves as a critical mechanism for advancing agricultural modernization and rural revitalization. Key drivers, such as agricultural modernization and industrial development, significantly promote land transfer, particularly in coastal areas, while challenges like non-standardized contracts and inadequate farmer protections persist. The study proposes targeted strategies, including strengthening agricultural modernization, enhancing farmer income through non-agricultural industries, and improving institutional frameworks to stabilize the land transfer market. Additionally, it highlights the potential role of rural social workers in addressing contentious issues such as women’s rights, displacement, and access to resources, ensuring equitable and sustainable rural development. These measures aim to accelerate agricultural modernization, promote rural economic growth, and enhance the competitiveness and social well-being of rural areas, providing a robust foundation for policy formulation. Full article

Soil particle size distribution is a critical parameter in geotechnical and hydraulic engineering, particularly in applications such as dam seepage monitoring, building foundation assessments, and sediment transport. This study presents a novel algorithm for estimating soil particle sizes by analyzing their falling velocities in water, combining optical flow computation with chaotic motion analysis. To address the limitations of the classical Horn and Schunck method, particularly its sensitivity to large displacements and brightness variations, we introduced a coarse-to-fine warping strategy, an image decomposition step to separate dominant structures from fine textures, and the Charbonnier penalty function. The improved model achieved competitive accuracy compared to advanced optical flow algorithms. To manage turbulence and motion noise during particle settling, we incorporated a global flow analysis framework using streaklines, streak flow, and potential functions. This enabled the segmentation of laminar, turbulent, and rebound flow regions without requiring individual particle tracking. Soil particle sizes were then back-calculated from laminar flow velocities using Stokes’ Law. Experimental results confirmed the method’s accuracy for particle sizes ranging from 20 mm to 0.7 mm, significantly extending the measurable range of Sedimaging systems. The proposed approach shows strong potential for integration into dam-related particle monitoring applications and building-related monitoring systems requiring fine-resolution analysis. Full article

For many years, the temporal distribution of pigs in the Pleistocene of Europe drew little attention. This changed when, what became known as, the “suid gap” hypo-thesis was published. Subsequent publications added elements to this hypothesis, while others questioned the hypothesis and even the existence of a “suid gap”. In its most complete form the hypothesis consists of a chain of arguments: (1) pigs are r-selected (a life history trait), (2) therefore fossils of their deciduous teeth are particularly abundant, (3) because being r-selected, pigs are abundant, (4) sites without pig fossils are sure indicators of their absence, (5) at 1.8 Ma, humans dispersed into Europe driving Sus strozzii to extinction in all the continent by competitive displacement, but not in the Middle East, (6) around 1.2 Ma pigs appeared again in Europe, either Sus strozzii, coming from the Middle East, or another species. The proposed link between human and pig ecology increased the interest of this hypothesis. Recently parts of this hypothesis were questioned and a polemic arose. It is the aim of this paper to review the literature and arguments used in favour and against this “suid gap” hypothesis. The hypothesis is rejected, but the life history traits of pigs may prove to be of interest for comparison with humans. Full article

Reforestation is widely promoted as a nature-based solution for climate change, yet its unintended consequences, such as deforestation leakage, remain under-investigated. This study provides empirical evidence of reforestation-induced leakage in the Brazilian Amazon, using municipality-level panel data from 2000 to 2023 and spatial Durbin panel models to estimate both the magnitude and spatial reach of agricultural displacement. Despite the positive local effects of reforestation projects, we found a significant displacement of deforestation to the vicinity of municipalities. We estimated a statistically significant deforestation leakage effect of approximately 12% from the reforested area, due to the agricultural displacement of cattle ranching activities. Spatial spillovers are strongest within a 150 km radius and within two years after reforestation onset. Sensitivity tests using alternative spatial weight matrices, including distance decay and land rent-weighted specifications, confirm the robustness of these findings. Livestock intensification, proxied by cattle stocking rates, does not significantly mitigate displacement effects, challenging assumptions about land sparing benefits. These results suggest that current carbon market protocols (e.g., Verra, ART-TREES) may improve their leakage analysis to avoid under- or over-estimating net carbon benefits. Incorporating spatial econometric evidence into offset methodologies and reforestation planning can improve climate policy integrity and reduce unintended environmental trade-offs. Full article

Ensuring food security is essential for achieving sustainable global development, requiring a balance between sufficient food production and maintaining its safety and nutritional value. However, this objective faces considerable challenges due to the infiltration of toxic metal species into the food supply. Heavy metals and metalloids, depending on their molecular form and daily dose, exhibit varying degrees of toxicity, making the precise identification of their species essential for assessing their impact on human health and the environment. This study focuses on identifying the primary anthropogenic sources and dissemination pathways of heavy metal pollutants, with an emphasis on their speciation and bioavailability. It examines how toxic metal species, such as Pb²⁺, Cd²⁺, Hg²⁺, and various arsenic species (AsIII and AsV), infiltrate ecosystems, bioaccumulate within the food chain, and ultimately compromise food safety and nutritional value. Furthermore, the research explores the physiological and biochemical disruptions caused by these toxic metal species, including the displacement of essential ions from enzymatic active sites and transport proteins due to competitive binding by pollutants, oxidative stress induced by reactive oxygen species generation, and cellular dysfunction affecting metabolic pathways and signaling cascades, all of which contribute to both chronic and acute health conditions. By providing a detailed analysis of exposure routes and toxicological processes, this paper highlights the far-reaching consequences of heavy metal contamination on public health and agricultural sustainability. Special attention is given to the need for precise terminology, as the toxicity of metals is inherently linked to their daily dose and chemical species rather than their elemental form. Finally, this study advocates for integrated, multidisciplinary strategies aimed at mitigating these risks, enhancing ecosystem stability, and ensuring long-term food security in the face of environmental challenges. Full article

Background/Objectives: High-performance affinity chromatography (HPAC) was used to investigate the binding affinity of a series of synthetic cannabinoids, a widely abused class of new psychoactive substances, to human serum albumin (HSA) and obtain insights into the binding sites. To better understand the recognition mechanisms, molecular docking studies were conducted. Methods: Binding affinity was assessed through zonal elution approach Additionally, displacement chromatography with site-specific probes provided insights into the HSA binding sites of five synthetic cannabinoids. Results: That these drugs exhibit extensive binding to HSA, with values ranging from 98.7% to 99.9%. Competition for site I was observed between warfarin and four synthetic cannabinoids (5F-AMB, AB-PINACA, AMB-FUBINACA, and AB-CHMINACA). Furthermore, AB-CHMINACA also competed with L-tryptophan for site II. The binding affinity of all synthetic cannabinoids increased in the presence of (S)-ibuprofen. Molecular docking studies supported the experimental findings, reinforcing the insights gained. Conclusions: The key novelty of this study lies in analyzing, for the first time, the binding affinity of synthetic cannabinoids to HSA through HPAC and molecular docking. These results may improve our understanding of their toxicokinetic behavior and help in predicting possible competitive interactions that could influence HSA binding and, consequently, their activity and toxicity. This study is the first to describe the binding affinity of synthetic cannabinoids to HSA, elucidate their recognition mechanisms, identify binding sites, and characterize their interactions with the protein. Full article