Search Results (30,057)

Bacterial spot of the pepper (BSP) and the tomato (BST) caused by multiple Xanthomonas spp. remains a major constraint to production of both crops worldwide. The widespread breakdown of dominant resistance genes, such as Bs2, due to the emergence of virulent races, like Xanthomonas euvesicatoria P6, has underscored the need for more durable, non-race-specific resistance. The recessive genes, bs5; bs6; and bs8, have emerged as promising alternatives, conferring broad-spectrum resistance without triggering a hypersensitive response. In this study, we systematically evaluated the individual and combinatorial effects of these three recessive resistance genes against three Xanthomonas species, X. euvesicatoria (Xe), X. hortorum pv. gardneri (Xhg), and X. perforans (Xp). Using near-isogenic lines (NILs) developed in the susceptible Early Calwonder (ECW) background, we assessed the in planta bacterial population growth and symptom development across a panel of eight genotypes carrying different gene combinations. Our results demonstrate that bs5, particularly when combined with either bs6 or bs8, significantly reduces bacterial growth and disease severity across all three Xanthomonas species. The triple-stacked line (ECW568 (i.e., bs5, bs6, and bs8)) consistently displayed the strongest suppression of pathogen proliferation and symptom development. By contrast, bs6 and bs8, alone or in combination, were largely ineffective. In some cases, combining bs6 with bs8 was less effective than bs8 alone. These findings reinforce the central role of bs5 in conferring quantitative resistance and highlight the additive benefit of pyramiding recessive resistance genes. Furthermore, we have demonstrated that these recessive resistance genes are effective in limiting the ability of the emerging pathogen, X. perforans, to grow in planta, and thus are predicted to offer a high level of resistance in the field. Our work provides key insights for breeding durable, broad-spectrum resistance into commercial pepper cultivars and offers a framework for integrated disease management strategies in the face of rapidly evolving bacterial pathogens Full article

This on-farm study explored the effects of diverse pasture systems and seaweed-based bio-stimulants (AgriSea NZ Seaweed Products, Paeroa, New Zealand) on sheep performance, metabolic health, milk composition, and carcass characteristics. A 3 × 2 factorial design was used to compare three pasture systems; ryegrass-white clover (RW), a 23-species diverse mix (DI), and functionally diverse strip swards (ST), with (SW) or without (CO) a seaweed-based bio-stimulant. Ninety pregnant ewes were stratified by live weight and allocated across six treatment groups (15 ewes per treatment). Lambing occurred on treatment paddocks. At weaning, 90 lambs (15 per treatment) were selected based on body weight and sex balance to continue through to finishing. Pasture chemical composition differed among treatments: ST had lower fibre (neutral detergent fibre, NDF; acid detergent fibre, ADF) than RW and DI, while SW increased dry matter digestibility (DMD) and metabolisable energy (ME), and reduced NDF and ADF (p < 0.05). Strip pastures improved lamb average daily gain (ADG) by 17% from lambing to weaning compared to DI, and by 14% from weaning to finishing compared to RW (p < 0.05). Seaweed-based bio-stimulant treatment enhanced lamb ADG by up to 12% and improved carcass traits, including loin and shoulder yields (p < 0.05). Ewes and lambs on seaweed-treated pastures exhibited lower serum non-esterified fatty acid (NEFA) concentrations (p < 0.05), indicating better energy balance. Milk from ST and/or SW treated ewes had elevated omega-6 fatty acids and essential amino acids, suggesting enhanced nutritional value. These findings demonstrate that combining botanical diversity with natural bio-stimulants can improve animal growth, metabolic health, and product quality, offering a promising strategy for sustainable and welfare-oriented sheep production systems. Full article

This study aimed to develop an effective method to overcome the challenge of straw return in cold-region soil. We systematically investigated the synergistic mechanism of montmorillonite (MMT) and composite amino acid (CAA) on straw humification and carbon sequestration through a low-temperature litterbag field experiment. The results indicate that the combined treatment (MMT-CAA) significantly increased the decomposition rate of straw by 42.1% compared to the control (CK), with MMT showing particular efficacy in lignin degradation (28.3% reduction), while the CAA preferentially decomposed cellulose (19.7% reduction). An FTIR analysis of the decomposition products confirmed these findings. Water-soluble organic carbon (WEOC) and its three-dimensional fluorescence spectra exhibited a 25.0% increase in MMT-CAA and enhanced aromaticity of humic acid-like substances. Humic substances and their ¹³C-NMR revealed that MMT-CAA enhanced humic acid formation and molecular stability by 31.4% (with a 47.8% increase in aromaticity). A further redundancy analysis and symbiotic network of microorganisms demonstrated that MMT-CAA increased the abundance of lignocellulose-degrading phyla (Actinomycetes and Stramenomycetes) and the formation of a complex co-degradation network. Field corn planting trials indicated that MMT-CAA increased plant height by 55.1%, stem thickness by 58.7%, leaf area by 70.2%, and the SPAD value by 41.1%. Additionally, MMT significantly reduced CO₂ and N₂O emission fluxes by 35.6% and 15.8%, respectively, while MMT-CAA increased CH₄ uptake fluxes by 13.4%. This study presents an innovative strategy, providing mechanistic insights and practical solutions to synergistically address the challenges of slow straw decomposition and carbon loss in cold regions. Full article

Simulating nonlinear classical dynamics on a quantum computer is an inherently challenging task due to the linear operator formulation of quantum mechanics. In this work, we provide a systematic approach to alleviate this difficulty by developing an explicit quantum algorithm that implements the time evolution of a second-order time-discretized version of the Lorenz model. The Lorenz model is a celebrated system of nonlinear ordinary differential equations that has been extensively studied in the contexts of climate science, fluid dynamics, and chaos theory. Our algorithm possesses a recursive structure and requires only a linear number of copies of the initial state with respect to the number of integration time-steps. This provides a significant improvement over previous approaches, while preserving the characteristic quantum speed-up in terms of the dimensionality of the underlying differential equations system, which similar time-marching quantum algorithms have previously demonstrated. Notably, by classically implementing the proposed algorithm, we showcase that it accurately captures the structural characteristics of the Lorenz system, reproducing both regular attractors–limit cycles–and the chaotic attractor within the chosen parameter regime. Full article

The electric arc furnace (EAF) is a key technology in the steel production industry, particularly for recycling scrap iron. It plays a crucial role in the shift to low-carbon metallurgy, responding to the growing demand for more sustainable production methods. Alongside its environmental and energy benefits, the EAF process generates significant amounts of solid by-products, including dust (EAFD) and slag (EAFS). These wastes are not only rich in base metals but also contain critical elements, which have attracted increasing scientific and industrial interest. Depending on the waste type, key metals such as zinc (from EAFD) and chromium, vanadium, and titanium (from EAFS) are targeted for recovery. This review examines the chemical and phase compositions of these wastes, various leaching techniques (often combined with pretreatment stages), and methods for final metal recovery, either in their pure form or as compounds. Key challenges in hydrometallurgical routes include chloride contamination, the dissolution of refractory zinc ferrite, and impurity management. Despite current limited industrial adoption, hydrometallurgical approaches show significant promise as efficient and environmentally friendly solutions for resource recycling, offering high-purity metal recovery. Full article

Social media promotion of harmful products (e.g., combustible tobacco) poses a public health threat. However, strategies that amplify exposure to and engagement with such content remain understudied. This study aims to characterize strategies boosting cigar, little cigar, and cigarillo (CLCC) marketing visibility, referrals, and engagement on Instagram. Using keyword rules, we collected publicly available CLCC-related Instagram posts from CrowdTangle for a six-year period from August 2016 to October 2021. Posts were categorized as commercial (e.g., posts by tobacco brands or vendors) or organic and were coded for consumer engagement (CE) strategies (e.g., presence of prompts to like/share) using a combination of machine learning methods and human coding. Temporal engagement trends were analyzed using metadata. A total of 320,488 CLCC-related public posts were collected, with 44.6% (n = 142,875) identified as overtly commercial. Of these, 33.5% (n = 47,832) contained CE cues, including discounts and giveaways for tagging peers, liking, commenting, or following CLCC brands and spokesperson/influencers accounts, as well as calls to participate in contests and polls. Overtly commercial CE messages consistently garnered more comments per post and likes per post than non-CE commercial posts. There was a significant upward trend in the rate of comments on CE posts, suggesting growing effectiveness in eliciting user interaction. The proliferation of and high level of engagement with cigar-related promotional messages on Instagram demonstrate the need for public health surveillance and regulation of the evolving strategies promoting CLCC marketing exposure, reach, and engagement on social media. Full article

The rapid conversion of cropland into construction land in China poses an increasing threat to national food security. Using high-resolution (30 m) land-use data from 2000 to 2020, obtained from the Resource and Environment Science and Data Center, along with spatial analytical methods such as land-use transition metrics and centroid-based migration models, this study identifies the spatio-temporal evolution and spatial migration patterns of construction land expansion and cropland loss. Combined with multi-source data, including socioeconomic, environmental, and topographic variables, this study employs a geographically weighted regression (GWR) model to explore the spatially heterogeneous driving mechanisms of two key indicators: the dependency of construction land expansion on cropland (DEP) and the contribution of cropland loss to construction land (CON). The results reveal that cropland has generally increased in the west and decreased in the east, reflecting expansion in underdeveloped areas and shrinkage in wealthier regions. In contrast, construction land expansion shows polycentric and stage-specific characteristics. Both DEP and CON remain high in major grain-producing areas like the North China Plain. Among the influencing factors, the non-grain production rate strongly inhibits DEP, while chemical fertilizer use significantly promotes CON. The urbanization rate shows a southwest-to-northeast increasing inhibitory effect on DEP and a northeast-to-southwest increasing promoting effect on CON. Local fiscal expenditure and land degradation demonstrate spatially heterogeneous effects. These differences highlight the conflict between low-cost land development priorities and cropland protection policies. Full article

The impact of final surface preparation immediately prior to hydrogen content measurements in aluminum alloy samples was investigated using thermal desorption analysis (TDA). Samples ground in water showed an apparent hydrogen signal. Glow-discharge optical emission spectroscopy (GDOES) confirmed that the analyzed hydrogen originated from the subsurface layer. X-ray photoelectron spectroscopy (XPS) revealed the presence of a thin aluminum oxide/hydroxide layer on the surface. Formation of these compounds indicates that hydrogen was introduced into the material by the reaction of oxide-free aluminum with water molecules during the grinding, followed by its entrapment at near-surface interstitial lattice sites. Chemical pickling in concentrated nitric acid and combined grinding, chemical pickling, and electrochemical polishing approaches are proposed as proper surface-preparation techniques for samples without and with adherent corrosion products, respectively. Full article

This study investigated the effects of probiotic supplementation on performance, egg quality, antioxidant capacity, gut morphology, fecal microbiota, and bone morphology in Lohmann Brown laying hens aged 44 weeks over a 16-week period. Ninety-six hens were randomly divided into control and probiotic groups (n = 48 each). The probiotic group received probiotic supplement containing Lactobacillus acidophilus KUEN 1607 and Pediococcus acidilactici KUEN 1608 via drinking water at 0.5%. Probiotic supplementation significantly improved feed conversion ratio (FCR), eggshell strength and thickness, and albumen quality (p < 0.001) and reduced the incidence of cracked and shell-less eggs (p < 0.05). Yolk and serum cholesterol levels decreased (p < 0.001), and antioxidant parameters improved, along with elevated serum IgG (p < 0.001). Histological analysis showed an increased ratio of villus height to crypt depth (p < 0.001) in the jejunum, indicating enhanced intestinal health. Fecal samples revealed increased Lactobacillus spp. and reduced coliform counts (p < 0.001), suggesting improved gut microbiota balance. While bone volume and surface area showed no significant difference, 3D geometric morphometric analysis identified subtle shape changes in long bones, especially the femur and tibiotarsus. These findings demonstrate that the selected probiotic combination supports nutrient utilization, egg quality, gut integrity, immune status, and skeletal health, offering a sustainable strategy to enhance productivity and welfare in laying hens. Full article

This study offers a detailed environmental, energy, and economic evaluation of thermal modernisation options for an existing single-family home in southern Poland. A total of 24 variants, combining different heat sources (solid fuel, biomass, natural gas, and heat pumps) with various levels of building insulation, were analysed using energy performance certification methods. Results show that, from an energy perspective, the most advantageous scenarios are those utilising brine-to-water or air-to-water heat pumps supported by photovoltaic systems, reaching final energy demands as low as 43.5 kWh/m²year and primary energy demands of 41.1 kWh/m²year. Biomass boilers coupled with solar collectors delivered the highest renewable energy share (up to 99.2%); however, they resulted in less notable reductions in primary energy. Environmentally, all heat pump options removed local particulate emissions, with CO₂ reductions of up to 87.5% compared to the baseline; biomass systems attained 100% CO₂ reduction owing to renewable fuels. Economically, biomass boilers had the lowest unit energy production costs, while PV-assisted heat pumps faced the highest overall costs despite their superior environmental benefits. The findings highlight the trade-offs between ecological advantages, energy efficiency, and investment costs, offering a decision-making framework for the modernisation of sustainable residential heating systems. Full article

The widespread prevalence of Salmonella underscores the urgent need for rapid, sensitive, and reliable detection methods to ensure food safety and protection of public health. In this study, we successfully developed an integrated detection system that combines immunomagnetic separation with surface plasmon resonance (SPR) analysis. This system achieved high capture efficiencies, exceeding 96.04% in phosphate-buffered saline and over 91.66% in milk samples artificially spiked with S. Typhimurium at concentrations below 4.2 × 10⁴ CFU/mL. However, direct SPR detection of the isolated S. Typhimurium showed limited sensitivity, with a limit of detection (LOD) of 4.2 × 10⁷ CFU/mL. Incorporating a sandwich assay with antibody-conjugated gold nanoparticles significantly enhanced sensitivity, lowering the LOD by six orders of magnitude to 4.2 × 10¹ CFU/mL. The whole integrated process, integrating immunomagnetic separation with SPR analysis, was completed within 50 min. These results demonstrate that this AuNP-enhanced SPR platform offers both the rapidity and sensitivity essential for effective monitoring of food safety and traceability in Salmonella-related foodborne outbreaks, particularly in products such as milk. Full article

Plants are constantly exposed to various environmental challenges, such as drought, flooding, heavy metal toxicity, and pathogen attacks. To cope with these stresses, they employ several adaptive strategies. This review highlights the potential of plant-derived smoke (PDS) solution as a natural biostimulant for improving plant health and resilience, contributing to both crop productivity and ecological restoration under abiotic and biotic stress conditions. Mitigating effects of PDS solution against various stresses were observed at morphological, physiological, and molecular levels in plants. PDS solution application involves strengthening the cell membrane by minimizing electrolyte leakage, which enhances cell membrane stability and stomatal conductance. The increased reactive-oxygen species were managed by the activation of the antioxidant system including ascorbate peroxidase, superoxide dismutase, and catalase to meet oxidative damage caused by challenging conditions imposed by flooding, drought, and heavy metal stress. PDS solution along with other by-products of fire, such as charred organic matter and ash, can enrich the soil by slightly increasing its pH and improving nutrient availability. Additionally, some studies indicated that PDS solution may influence phytohormonal pathways, particularly auxins and gibberellic acids, which can contribute to root development and enhance symbiotic interactions with soil microbes, including mycorrhizal fungi. These combined effects may support overall plant growth, though the extent of PDS contribution may vary depending on species and environmental conditions. This boost in plant growth contributes to protecting the plants against pathogens, which shows the role of PDS in enduring biotic stress. Collectively, PDS solution mitigates stress tolerance in plants via multifaceted changes, including the regulation of physico-chemical responses, enhancement of the antioxidant system, modulation of heavy metal speciation, and key adjustments of photosynthesis, respiration, cell membrane transport, and the antioxidant system at genomic/proteomic levels. This review focuses on the role of PDS solution in fortifying plants against environmental stresses. It is suggested that PDS solution, which already has been determined to be a biostimulant, has potential for the revival of plant growth and soil ecosystem under abiotic and biotic stresses. Full article

This study addresses the increasing demand for texture-modified seafood products suitable for elderly consumers by focusing on dried squid, a popular protein source. The aim was to optimize the softening and drying procedures to produce a dried squid product with improved chewability and quality. Fresh squid was pretreated using sodium bicarbonate or potassium carbonate solutions (0, 0.3, 0.6, and 0.9 mol/kg) and dried at 40 °C using either continuous (CD) or intermittent drying (ID) until the final moisture content reached 18.34 ± 0.44%. Hardness generally increased with higher alkaline concentrations, with the potassium carbonate-treated samples showing better softening effects. Based on standards for elderly-friendly foods targeting chewable hardness (10,000–50,000 N/m²), low water activity (<0.58), and limited color change (ΔE = 14.32), the optimal result was achieved with 0.3 mol/kg potassium carbonate and ID. Among the thin-layer drying models, the Midilli–Kucuk model showed the best fit, with the highest average R² (0.9974), and lowest SSE (0.0481) and RMSE (0.1688), effectively capturing the drying kinetics. Scanning electron microscopy (SEM) revealed smoother surfaces and consistent porosity in samples dried intermittently, indicating less structural degradation. Finite element analysis showed that ID improved internal moisture distribution, reduced surface crusting, and alleviated internal stresses. These results support mild alkaline soaking combined with ID as an effective strategy for enhancing dried squid quality for elderly individuals. Full article

The upper reaches of the Yangtze River face the challenge of balancing livestock development and ecological protection. As a significant livestock production region in China, optimizing the livestock ecological efficiency (LEE) of Sichuan Province (SP) is of strategic importance for regional sustainable development. Livestock carbon emissions and related pollution indices were utilized as undesirable output indicators within the super-efficiency SBM model to measure SP’s LEE over the 2010–2022 period. Kernel density estimation was combined with the Theil index to analyze spatiotemporal variation characteristics. A STIRPAT model was constructed to explore the influencing factors of SP’s LEE, and a grey forecasting GM (1,1) model was employed for prediction. Key findings reveal the following: (1) LEE increased by 25.9%, with high-efficiency regions expanding from 19.0% to 57.1%; (2) regional disparities persist, driven by labor redundancy and environmental governance gaps; (3) per capita GDP, industrial agglomeration, and technology advancement significantly promoted efficiency, while government subsidies and carbon intensity suppressed it. Projections show LEE reaching 0.923 by 2035. Key recommendations include the following: (1) implementing region-specific strategies for resource optimization, (2) restructuring agricultural subsidies to incentivize emission reduction, and (3) promoting cross-regional technology diffusion. These provide actionable pathways for sustainable livestock management in ecologically fragile zones. Full article

Multimaterial 3D printing is transforming the landscape of additive manufacturing, enabling the production of advanced, functional parts with tailored properties for sectors like automotive, aerospace, and engineering. However, achieving strong interlayer adhesion between different polymers remains a significant challenge, limiting the mechanical reliability. This study investigates adhesion properties of widely used materials—polycarbonate (PC), acrylonitrile styrene acrylate (ASA), polylactic acid (PLA), and polyethylene terephthalate glycol (PETG)—and enhances mechanical performance of structural joints through optimized interlayer bonding techniques. Using the Material Extrusion (MEX) method, tensile testing was employed to evaluate the mechanical strength of joints by co-depositing and bonding material layers during the printing process. The results demonstrate that specific material combinations and joint design strategies, particularly increasing the interfacial contact area and applying interlayer bonding pressure, significantly enhance tensile strength. For instance, the strength of PC/PTEG composite joints increased from 15.2 MPa (standard joint) to 29.9 MPa (interlayer bonding strategy), nearly doubling the bond strength. These findings provide valuable insights into the behavior of multimaterial joints and propose practical approaches for improving the durability and functionality of 3D-printed structures. This research lays the groundwork for advancing multimaterial additive manufacturing, with implications for high-performance applications in engineering, aerospace, and beyond. Full article