Search Results (1,891)

Lychee (Lychee chinensis Sonn.) is a tropical fruit highly appreciated for its vivid red color, sweet flavor, and nutritional properties. However, it is highly perishable, with postharvest losses often due to oxidative browning and dehydration. This study evaluated the organic olive oil coating (OC), a natural lipidic system with the potential to act as a precursor for organogel development, combined with polyamide/polyethylene (PA/PE) packaging under passive modified atmosphere. Fruits were harvested at commercial maturity and divided into two groups: OC-treated and untreated control (CTR). Both groups were stored at 5 ± 1 °C and 90 ± 5% relative humidity and analyzed on days 0, 3, 6, and 9. The OC-treated fruits showed significantly better retention of physical, chemical, microbiological, and sensory qualities. The coating reduced oxidative stress and enzymatic browning, preserving color and firmness. The PA/PE packaging regulated gas exchange, lowering oxygen levels and delaying respiration and ripening. As a result, OC fruits had lower weight loss, a slower increase in browning index and maturity index, and better visual and sensory scores than the CTR group. This dual strategy proved effective in extending shelf life while maintaining the fruit’s appearance, flavor, and nutritional value. It represents a sustainable and natural approach to enhancing the postharvest stability of lychee. Full article

Consumer interest in vegetarian, ethical, and clean-label foods is reviving the use of plant-derived milk coagulants. Cardosins from Cynara cardunculus (“thistle”) are aspartic proteases with strong clotting activity, yet their technological impact in cheese remains under-explored. This study compared a commercial thistle extract (PC) with traditional bovine rennet rich in chymosin (AC) during manufacture and 60-day ripening of Caciotta cheese. Classical compositional assays (ripening index, texture profile, color, solubility) were integrated with scanning electron microscopy, three-dimensional surface reconstruction, and descriptive sensory analysis. AC cheeses displayed slower but sustained proteolysis, yielding a higher and more linear ripening index, softer body, greater solubility, and brighter, more yellow appearance. Imaging revealed a continuous protein matrix with uniformly distributed, larger pores, consistent with a dairy-like sensory profile dominated by milky and umami notes. Conversely, PC cheeses underwent rapid early proteolysis that plateaued, producing firmer, chewier curds with lower solubility and darker color. Micrographs showed a fragmented matrix with smaller, heterogeneous pores; sensory evaluation highlighted vegetal, bitter, and astringent attributes. The data demonstrate that thistle coagulant can successfully replace animal rennet but generates cheeses with distinct structural and sensory fingerprints. The optimization of process parameters is therefore required when targeting specific product styles. Full article

Background and clinical significance: Trichoblastomas are rare, mixed epithelial tumors with a mesenchymal component and hair follicle differentiation. Case presentation: Herein, we present a case report of a 51-year-old female patient presenting to the obstetrics and gynecology department with complaints of edema and erythema of the right Bartholin gland, and a lesion measuring 2 cm on the right lateral edge of the mons pubis, towards the inguinal fold. Marsupialization of the Bartholin gland was performed, as well as an incision into the pubo-inguinal lesion, which the patient depicted as grossly resembling an ingrown hair. Upon incision into the pubic–inguinal lesion, it was dark brown in color and spontaneously popped out of the subcutis, without an attempt at enucleation. Histology and subsequent immunohistochemistry of the lesion showed a blue basaloid tumor with an extensive pigment component located deep in the dermis that was sharply demarcated from the surrounding tissues. Conclusion: Immunohistochemistry was diffusely and strongly positive for epithelial markers; melanocytic markers were positive only in dendritic melanocytes dispersed within the tumors, and the proliferative index was low. As such, the tumor was identified as melanotrichoblastoma. Full article

Rhubarb, a medicinal herb in Gansu Province, China, undergoes significant quality changes during sun-drying. This study investigated color changes, drying kinetics, anthraquinone (AQ) content, metabolic profiles, and enzyme activity during the process. Results showed that drying induced enzymatic browning, with the browning index (BI) progressively increasing over extended drying periods (4–16 h) and with greater slice thickness (2–8 mm). Catalase (CAT) activity first decreased and then increased, while polyphenol oxidase (PPO) activity decreased throughout drying. Slice thickness significantly affected AQ content, with the highest in 2 mm slices and the lowest in 4 mm slices. The drying process followed a logarithmic model (R² = 0.99418, RMSE = 0.02310, and χ² = 0.0005). Metabolomics analysis identified 631 differential metabolites, with 8 key metabolites linked to flavonoid biosynthesis, phenylalanine biosynthesis, and tyrosine metabolism. Fifteen enzymes were involved in metabolite synthesis and decomposition, though some enzyme activity trends contradicted metabolite changes. This study provides insight into rhubarb drying mechanisms and a basis for optimizing the drying process. Full article

Foliar fertilization is an effective practice that improves both the yield and quality of maize, a crop with high and specific micronutrient demands. This study hypothesized that foliar application of Fe, Cu, Mn, Mo, Zn and B would improve grain size and quality in GS210 maize compared to the control. The single-factor field experiment was conducted in 2023–2024 on Haplic Cambisol (Eutric) soil, under a variety of meteorological conditions. The application of Zn and B fertilizers significantly increased the soil plant analysis development (SPAD) index. Yield components (number of grains per ear, thousand-grain weight) and grain yield increased significantly following Zn foliar application compared to the control. Zn application increased grain yield by 0.59 t ha⁻¹ and 0.49 t ha⁻¹ in 2023 and 2024, respectively. Smaller but beneficial effects were observed with Cu and B applications. In contrast, the effects of fertilization with other micronutrients (Fe, Mn, Mo) were less pronounced than anticipated. Biochemical analyses revealed that foliar fertilization with Fe, Cu and Mo increased total phenolic content and antioxidant capacity, while Fe and Mo enhanced carotenoid accumulation, and Cu and B significantly influenced grain color parameters. The study highlights the potential of foliar fertilization to improve maize performance and grain quality, despite possible antagonisms between micronutrients. Full article

Fish coloration plays an important role in reproduction and camouflage, yet capturing color variation under field conditions remains challenging. We present a standardized, semi-automated protocol for measuring body coloration in the popular model fish threespine stickleback (Gasterosteus aculeatus). Individuals are photographed in a controlled light box within minutes of capture, and color is sampled from eight anatomically defined standard sites in human-perception-based CIELAB space. Analyses combine univariate color metrics, multivariate statistics, and the ΔE* perceptual difference index to detect subtle shifts in hue and brightness. Validation on pre-spawning fish shows the method reliably distinguishes males and females well before full breeding colors develop. Although it currently omits ultraviolet signals and fine-scale patterning, the approach scales efficiently to large sample sizes and varying lighting conditions, making it well suited for population-level surveys of camouflage dynamics, sexual dimorphism, and environmental influences on coloration in sticklebacks. Full article

Background: Type 2 diabetes (T2D) is a growing public health concern, particularly in low- and middle-income countries. Although prediabetes is a major risk factor for T2D, it remains largely underdiagnosed and untreated. Structured lifestyle interventions have proven effective in preventing diabetes, but their feasibility within the Brazilian public health system remains unclear. Methods: This multicenter pilot randomized controlled trial assessed the feasibility of a culturally adapted lifestyle intervention (PROVEN-DIA) across the five regions of Brazil. A total of 220 adults at high risk for T2D were randomized to an intervention group or a control group (usual care) and followed for three months. Both groups received similar educational content on healthy eating and physical activity, but the intervention group participated in a structured and personalized lifestyle program with regular follow-up sessions. The primary outcome was adherence to dietary recommendations, assessed using the BALANCE Index—a validated dietary score (range: 0–40) based on the Brazilian Cardioprotective Diet that classifies foods into color-coded groups according to nutritional quality—along with engagement in moderate-to-vigorous physical activity (MVPA). Secondary outcomes included diet quality (DQIR), anthropometric and metabolic parameters. Results: Feasibility was demonstrated by a 93.2% retention rate (n = 205). There was no significant difference in the primary outcome (simultaneous improvement in diet and MVPA). However, the PROVEN-DIA group exhibited significantly greater improvements in diet quality, with a 2.8-point increase in the BALANCE Index (vs. 0.5 in the control, p = 0.03), and a significant improvement in the DQIR (p < 0.001). No significant differences between groups were observed in MVPA, HbA1C, glycaemia, or body weight. Conclusions: The PROVEN-DIA intervention proved feasible within the Brazilian public health context, resulting in significant improvements in dietary quality among individuals at high risk for T2D. A larger trial with longer follow-up is warranted to evaluate its effectiveness in preventing the progression to diabetes. However, to enhance physical activity outcomes, specific adaptations and targeted strategies may be required to better support participant engagement in exercise. Full article

Noncommunicable diseases (NCDs) like diabetes and cancer drive demand for therapeutic functional foods. This study developed freeze-dried fermented camel milk (FCM) with Ajwa date pulp (ADP), evaluating its physical and functional properties, probiotic survival, and potential benefits for diabetes and cancer. To achieve this target, six FCM formulations were prepared using ABT-5 starter culture (containing Lactobacillus acidophilus, Bifidobacterium bifidum, and Streptococcus thermophilus) with or without Lacticaseibacillus rhamnosus B-1937 and ADP (12% or 15%). The samples were freeze-dried, and their functional properties, such as water activity, dispersibility, water absorption capacity, water absorption index, water solubility index, insolubility index, and sedimentation, were assessed. Reconstitution properties such as density, flowability, air content, porosity, loose bulk density, packed bulk density, particle density, carrier index, Hausner ratio, porosity, and density were examined. In addition, color and probiotic survivability under simulated gastrointestinal conditions were analyzed. Also, antidiabetic potential was assessed via α-amylase and α-glucosidase inhibition assays, while cytotoxicity was evaluated using the MTT assay on Caco-2 cells. The results show that ADP supplementation significantly improved dispersibility (up to 72.73% in FCM15D+L). These improvements are attributed to changes in particle size distribution and increased carbohydrate and mineral content, which facilitate powder rehydration and reduce clumping. All FCM variants demonstrated low water activity (0.196–0.226), indicating good potential for shelf stability. The reconstitution properties revealed that FCM powders with ADP had higher bulk and packed densities but lower particle density and porosity than controls. Including ADP reduced interstitial air and increased occluded air within the powders, which may minimize oxidation risks and improve packaging efficiency. ADP incorporation resulted in a significant decrease in lightness (L*) and increases in redness (a*) and yellowness (b*), with greater pigment and phenolic content at higher ADP levels. These changes reflect the natural colorants and browning reactions associated with ADP, leading to a more intense and visually distinct product. Probiotic survivability was higher in ADP-fortified samples, with L. acidophilus and B. bifidum showing resilience in intestinal conditions. The FCM15D+L formulation exhibited potent antidiabetic effects, with IC₅₀ values of 111.43 μg mL⁻¹ for α-amylase and 77.21 μg mL⁻¹ for α-glucosidase activities, though lower than control FCM (8.37 and 10.74 μg mL⁻¹, respectively). Cytotoxicity against Caco-2 cells was most potent in non-ADP samples (IC₅₀: 82.22 μg mL⁻¹ for FCM), suggesting ADP and L. rhamnosus may reduce antiproliferative effects due to proteolytic activity. In conclusion, the study demonstrates that ADP-enriched FCM is a promising functional food with enhanced probiotic viability, antidiabetic potential, and desirable physical properties. This work highlights the potential of camel milk and date synergies in combating some NCDs in vitro, suggesting potential for functional food application. Full article

Timely and accurate diagnosis of gastrointestinal diseases (GIDs) remains a critical bottleneck in clinical endoscopy, particularly due to the limited contrast and sensitivity of conventional white light imaging (WLI) in detecting early-stage mucosal abnormalities. To overcome this, this research presents Spectrum Aided Vision Enhancer (SAVE), an innovative, software-driven framework that transforms standard WLI into high-fidelity hyperspectral imaging (HSI) and simulated narrow-band imaging (NBI) without any hardware modification. SAVE leverages advanced spectral reconstruction techniques, including Macbeth Color Checker-based calibration, principal component analysis (PCA), and multivariate polynomial regression, achieving a root mean square error (RMSE) of 0.056 and structural similarity index (SSIM) exceeding 90%. Trained and validated on the Kvasir v2 dataset (n = 6490) using deep learning models like ResNet-50, ResNet-101, EfficientNet-B2, both EfficientNet-B5 and EfficientNetV2-B0 were used to assess diagnostic performance across six key GI conditions. Results demonstrated that SAVE enhanced imagery and consistently outperformed raw WLI across precision, recall, and F1-score metrics, with EfficientNet-B2 and EfficientNetV2-B0 achieving the highest classification accuracy. Notably, this performance gain was achieved without the need for specialized imaging hardware. These findings highlight SAVE as a transformative solution for augmenting GI diagnostics, with the potential to significantly improve early detection, streamline clinical workflows, and broaden access to advanced imaging especially in resource constrained settings. Full article

Low glycemic index (GI) cookie bars were prepared with soft wheat flour substituted with 10–50% soybean flour and 10–50% resistant starch. The effects of increased levels of soybean flour and resistant starch on the quality of low glycemic index cookie bars were investigated (i.e., moisture, cookie spread, texture (breaking force), surface color, and in vitro starch digestibility). It was found that increasing soybean flour substitution increased the breaking force, moisture, protein content, and yellowish color of the low GI cookie bars but decreased the cookie bar spread and the lightness of the cookie bars (p < 0.05). The addition of soybean flour and resistant starch by up to 50% did not significantly change the in vitro starch digestibility of the cookie bars. The overall acceptability of the cookie bars was lower when the soybean flour blend went beyond 10%. When soft wheat flour in the cookie bar formulation was replaced at the following levels (10%, 30%, and 50%) by resistant starch, the cookie spread and lightness of the cookie bars increased but the breaking force was decreased along with the yellowish color (p < 0.05). When resistant starch was combined with soft wheat flour at levels of up to 50%, this significantly increased the content of total dietary fiber and spread ratio of cookie bars. Sensorial analysis showed that resistant starch presence had an acceptable impact on overall acceptability of the low GI cookie bars. Resistant starch represents a viable dietary fiber source when substituted for 50% of soft wheat flour in formulations. While this substitution may result in increased spread ratio and decreased crispness in cookie bars, the addition of 10% soybean flour can mitigate these textural changes. Full article

Background/Objectives: Global warming and rising CO₂ concentrations pose significant challenges to plant systems. Amid these pressures, this study contributes to understanding how tropical species respond by simultaneously evaluating reproductive and genetic traits. It specifically investigates the effects of maternal exposure to warming and elevated CO₂ on progeny physiology, genetic diversity, and population structure in Stylosanthes capitata, a resilient forage legume native to Brazil. Methods: Maternal plants were cultivated under controlled treatments, including ambient conditions (control), elevated CO₂ at 600 ppm (eCO₂), elevated temperature at +2 °C (eTE), and their combined exposure (eTEeCO₂), within a Trop-T-FACE field facility (Temperature Free-Air Controlled Enhancement and Free-Air Carbon Dioxide Enrichment). Seed traits (seeds per inflorescence, hundred-seed mass, abortion, non-viable seeds, coat color, germination at 32, 40, 71 weeks) and abnormal seedling rates were quantified. Genetic diversity metrics included the average (A) and effective (Ae) number of alleles, observed (Ho) and expected (He) heterozygosity, and inbreeding coefficient (Fis). Population structure was assessed using Principal Coordinates Analysis (PCoA), Analysis of Molecular Variance (AMOVA), number of migrants per generation (Nm), and genetic differentiation index (Fst). Two- and three-way Analysis of Variance (ANOVA) were used to evaluate factor effects. Results: Compared to control conditions, warming increased seeds per inflorescence (+46%), reduced abortion (−42.9%), non-viable seeds (−57%), and altered coat color. The germination speed index (GSI +23.5%) and germination rate (Gr +11%) improved with warming; combined treatments decreased germination time (GT −9.6%). Storage preserved germination traits, with warming enhancing performance over time and reducing abnormal seedlings (−54.5%). Conversely, elevated CO₂ shortened GSI in late stages, impairing germination efficiency. Warming reduced Ae (−35%), He (−20%), and raised Fis (maternal 0.50, progeny 0.58), consistent with the species’ mixed mating system; A and Ho were unaffected. Allele frequency shifts suggested selective pressure under eTE. Warming induced slight structure in PCoA, and AMOVA detected 1% (maternal) and 9% (progeny) variation. Fst = 0.06 and Nm = 3.8 imply environmental influence without isolation. Conclusions: Warming significantly shapes seed quality, reproductive success, and genetic diversity in S. capitata. Improved reproduction and germination suggest adaptive advantages, but higher inbreeding and reduced diversity may constrain long-term resilience. The findings underscore the need for genetic monitoring and broader genetic bases in cultivars confronting environmental stressors. Full article

We propose a simulation-based design for a flexible color filter (FCF) using a lithium niobate metamaterial (LNM) to investigate its color filtering potential. The FCF is composed of three periodically arranged half-ellipse LN arrays on a polydimethylsiloxane (PDMS) substrate, denoted as LNM-1, LNM-2, and LNM-3. The electromagnetic responses of the FCF can be controlled by adjusting the periods of the LNMs. Our simulations predict high-quality (Q) factors in transmission spectra, ranging from 100 to 200 for LNM-1, 290 to 360 for LNM-2, and 140 to 300 for LNM-3. When the FCF is exposed to the surrounding environments with different refractive indexes, it exhibits a theoretical figure of merit (FOM) up to 900 RIU⁻¹ and a sensitivity reaching 130 nm/RIU. The electromagnetic field distributions reveal strong confinement within the LNM nanostructures, confirming an efficient light–matter interaction. These results indicate that the proposed LNM-based FCF presents a promising design concept for high-performance color sensing and filtering applications. Full article

Anchote (Coccinia abyssinica) is a neglected high-potential food and nutrition security tuber crop in Ethiopia. Phenotyping core germplasm collections using agro-morphological and physiological markers is essential for effective crop improvement and utilization. A total of 282 anchote germplasms were profiled using six qualitative and twenty-six quantitative agro-morphological and physiological traits. Augmented Block Design was used for the experiment at the Debre Zeit Agricultural Research Center. The chi-square test and Shannon diversity index indicated the presence of substantial phenotypic variation and diversity among the accessions based on the predominant qualitative traits studied. The quantitative agro-morphological and physiological traits showed wider variability and ranges for the accessions. The broad-sense heritability and genetic advance as a percentage of the mean were notably high for quantitative traits such as root yield, vine length, and leaf area index. A significantly positive correlation was observed among agronomically important traits such as root yield and root diameter as well as root yield and leaf area. The principal component analysis for qualitative and quantitative traits found that ten components explained 72.2% of the variation for qualitative traits, whereas nine components accounted for 69.96% of the variation in quantitative traits. The primary contributors to the variations are traits such as root (shape, flesh color, and yield), leaf (color, length, diameter, area) and fruit (length, diameter, and weight). Further, the accessions were grouped into two and three clusters based on qualitative and quantitative traits, respectively, indicating that quantitative characters better differentiated among the accessions. Similarly, the tanglegram showed little similarity between the qualitative and quantitative agro-morphological and physiological traits in clustering the accessions. These findings indicate the presence of sizable trait variation among the accessions that can be exploited as a selection marker to design and facilitate conservation and breeding strategies of anchote. Full article

The intense demand for alternatives to conventional plastics has increasingly motivated the development of biodegradable packaging. However, the ecological impact of these materials when discarded in natural settings has not yet been evaluated. Therefore, this study investigated the effects of films based on chia mucilage in different aquatic environments. The solubilization time varied according to water type, ranging from 40 min in ultrapure, deionized, and distilled water to 230 min in saline water. After solubilization, all water samples exhibited increased turbidity (from 1.04 to 15.73 NTU in deionized water) and apparent color (from 0 to 44 PCU in deionized water) as well as pH variations depending on ionic strength. Deionized water also showed the highest viscosity increase (>350 Pa·s at 1 s⁻¹). UV–Vis spectra revealed a moderate rise in absorbance between 236 and 260 nm, indicating organic compound release. Regarding phytotoxicity, the solubilized films had no toxic effect and promoted a biostimulating effect on root elongation, with Relative Germination Index values exceeding 140% in most samples. These results reinforce the potential of chia-based films for controlled disposal, particularly in low-salinity environments, while highlighting the importance of evaluating post-solubilization interactions with aquatic systems. Full article

To address the concerns of contrast deterioration, detail loss, and color distortion in images produced under haze conditions in scenarios such as intelligent driving and remote sensing detection, an algorithm for image defogging that combines Retinex theory and the dark channel prior is proposed in this paper. The method involves building a two-stage optimization framework: in the first stage, global contrast enhancement is achieved by Retinex preprocessing, which effectively improves the detail information regarding the dark area and the accuracy of the transmittance map and atmospheric light intensity estimation; in the second stage, an a priori compensation model for the dark channel is constructed, and a depth-map-guided transmittance correction mechanism is introduced to obtain a refined transmittance map. At the same time, the atmospheric light intensity is accurately calculated by the Otsu algorithm and edge constraints, which effectively suppresses the halo artifacts and color deviation of the sky region in the dark channel a priori defogging algorithm. The experiments based on self-collected data and public datasets show that the algorithm in this paper presents better detail preservation ability (the visible edge ratio is minimally improved by 0.1305) and color reproduction (the saturated pixel ratio is reduced to about 0) in the subjective evaluation, and the average gradient ratio of the objective indexes reaches a maximum value of 3.8009, which is improved by 36–56% compared with the classical DCP and Tarel algorithms. The method provides a robust image defogging solution for computer vision systems under complex meteorological conditions. Full article