Search Results (372)

The effect of periodical heatwaves and related thermal stratification in freshwater aquatic ecosystems has been a hot research issue. A large dataset of samples was generated from samples exposed to temporary thermal stratification in mesocosms mimicking shallow eutrophic freshwater lakes. Temperature regimes were based on IPCC climate warming scenarios, enabling simulation of future warming conditions. Surface oxygen levels reached 19.37 mg/L, while bottom layers dropped to 0.07 mg/L during stratification. Analysis by FlowCAM revealed dominance of Cyanobacteria under ambient conditions (up to 99.2%), while Cryptophyta (up to 98.9%) and Chlorophyta (up to 99.9%) were predominant in the A2 and A2+50% climate scenarios, respectively. We identified temperature changes and shifts in nutrient concentrations, particularly phosphate, as critical factors in microbial community composition. Furthermore, five distinct Microcystis morphospecies identified by FlowCAM-based analysis were associated with different microbial clusters. The combined use of imaging flow cytometry, which differentiates phytoplankton based on morphological parameters, and nanopore long-read sequencing analysis has shed light into the dynamics of microbial communities associated with different Microcystis morphospecies. In our observations, a peak of algicidal bacteria abundance often coincides with or is followed by a decline in the Cyanobacteria. These findings highlight the importance of species-level classification in the analysis of complex ecosystem interactions and the dynamics of algal blooms in freshwater bodies in response to anthropogenic effects and climate change. Full article

High-resolution mooring observations captured diverse upper-ocean responses during typhoon passage, showing strong agreement with satellite-derived sea surface temperature and salinity. Analysis indicates that significant wind-induced mixing drove pronounced near-surface cooling and salinity increases at the mooring site. This mixing enhancement was predominantly governed by rapid intensification of near-inertial shear in the surface layer, revealed by mooring observations. Unlike shear instability, near-inertial horizontal kinetic energy displays a unique vertical distribution, decreasing with depth before rising again. Interestingly, the subsurface peak in diurnal tidal energy coincides vertically with the minimum in near-inertial energy. While both barotropic tidal forcing and stratification changes negligibly influence diurnal tidal energy emergence, significant energy transfer occurs from near-inertial internal waves to the diurnal tide. This finding highlights a critical tide–wave interaction process and demonstrates energy cascading within the oceanic internal wave spectrum. Full article

Background: Often, neoadjuvant therapy, which relies on the induction of double-strand breaks (DSBs), is used prior to surgery to shrink tumors by inducing cancer cell apoptosis. However, recent studies have suggested that this treatment may also induce a fluctuating state between senescence and stemness in PA-1 embryonal carcinoma cells, potentially affecting therapeutic outcomes. Thus, the respective epigenetic pathways are up or downregulated over a time period of days. These fluctuations go hand in hand with changes in spatial DNA organization. Methods: By means of Single-Molecule Localization Microscopy in combination with mathematical evaluation tools for pointillist data sets, we investigated the organization of euchromatin and heterochromatin at the nanoscale on the third and fifth day after etoposide treatment. Results: Using fluorescently labeled antibodies against H3K9me3 (heterochromatin tri-methylation sites) and H3K4me3 (euchromatin tri-methylation sites), we found that the induction of DSBs led to the de-condensation of heterochromatin and compaction of euchromatin, with a peak effect on day 3 after the treatment. On day 3, we also observed the co-localization of euchromatin and heterochromatin, which have marks that usually occur in exclusive low-overlapping network-like compartments. The evaluation of the SMLM data using topological tools (persistent homology and persistent imaging) and principal component analysis, as well as the confocal microscopy analysis of H3K9me3- and H3K4me3-stained PA-1 cells, supported the findings that distinct shifts in euchromatin and heterochromatin organization took place in a subpopulation of these cells during the days after the treatment. Furthermore, by means of flow cytometry, it was shown that the rearrangements in chromatin organization coincided with the simultaneous upregulation of the stemness promotors OCT4A and SOX2 and senescence promotors p21Cip1 and p27. Conclusions: Our findings suggest potential applications to improve cancer therapy by inhibiting chromatin remodeling and preventing therapy-induced senescence. Full article

The honey bee is a crucial pollinator in urban ecosystems but faces multiple challenges, including habitat degradation, pollution, and parasitism by species such as the phorid fly, Apocephalus borealis Brues (Diptera: Phoridae). This study investigated honey bee abundance and the percentage of A. borealis parasitism in an urban environment in San Francisco, California. We monitored six sites weekly for six months using two sampling methods. Individual bees were weighed and observed for parasitoid emergence under controlled laboratory conditions. Our results indicate fluctuations in honey bee parasitism by A. borealis from September 2024 to May 2025, with four distinct peaks occurring in mid-September, February, late March, and early May. The highest parasitism rates exceeded 50% in early May, coinciding with increased temperatures and drops in relative humidity. These results suggest a potential link between abiotic conditions and parasitoid activity, highlighting the importance of long-term monitoring to understand the seasonal dynamics of host–parasite interactions in urban environments. Full article

By integrating EarthCARE W-band doppler cloud radar observations with GPM Ku/Ka-band dual-frequency precipitation radar data, this study constructs a novel global “pseudo tripe-frequency” radar coincidence dataset comprising 2886 coincidence events (about one-third of the events detected precipitation), aiming to systematically investigating band-dependent responses to cloud and precipitation structure. Results demonstrate that the W-band is highly sensitive to high-altitude cloud particles and snowfall (reflectivity < 0 dBZ), yet it experiences substantial signal attenuation under heavy precipitation conditions, and with low-altitude reflectivity reductions exceeding 50 dBZ, its probability density distribution is more widespread, with low-altitude peaks increasing first, and then decreasing as precipitation increases. In contrast, the Ku and Ka-band radars maintain relatively stable detection capabilities, with attenuation differences generally within 15 dBZ, but its probability density distribution exhibits multiple peaks. As the precipitation rate increases, the peak value of the dual-frequency ratio (Ka/W) gradually rises from approximately 10 dBZ to 20 dBZ, and can even reach up to 60 dBZ under heavy rainfall conditions. Several cases analyses reveal clear contrasts: In stratiform precipitation regions, W-band radar reflectivity is higher above the melting layer than below, whereas the opposite pattern is observed in the Ku and Ka bands. Doppler velocities exceeding 5 m s⁻¹ and precipitation rates surpassing 30 mm h⁻¹ exhibit strong positive correlations in convection-dominated regimes. Furthermore, the dataset confirms the impact of ice–water cloud phase interactions and terrain-induced precipitation variability, underscoring the complementary strengths of multi-frequency radar observations for capturing diverse precipitation processes. Full article

Despite being recognized as toxic in anaerobic systems, sulfide’s potential to enhance hydrogen fermentation via microbial modulation remains underexplored. This study evaluated the combined effects of sulfide concentration (0–800 mg S/L) and heat pretreatment on hydrogen production during dark fermentation (DF). Without pretreatment, hydrogen yield reached 83 ± 2 mL/g COD at 0 mg S/L but declined with increasing sulfide, becoming negligible at 800 mg S/L. In contrast, heat-pretreated inocula showed markedly improved performance: peak cumulative production (4628 ± 17 mL) and yield (231 ± 1 mL/g COD) were attained at 200 mg S/L, while the maximum production rate (1462 ± 64 mL/h) occurred at 400 mg S/L. These enhancements coincided with elevated acetic and butyric acids, indicating a metabolic shift toward hydrogen-producing pathways. The microbial analysis of heat-pretreated samples revealed an enrichment of Clostridium butyricum (from 73.1% to 87.5%) and Clostridium perfringens, which peaked at 13.5% at 400 mg S/L. This species contributed to butyric acid synthesis. At 800 mg S/L, Clostridium perfringens declined sharply to 0.6%, while non-hydrogenogenic Levilinea saccharolytica proliferated, correlating with reduced butyric acid and hydrogen output. These findings indicate that sulfide supplementation, when combined with heat pretreatment, selectively restructures microbial communities and metabolic pathways, enhancing DF performance. Full article

Microplastics (MPs) have emerged as pervasive environmental contaminants due to their widespread presence across various ecosystems, including their use in single-use plastic food ware and table salt dispensers. This issue coincides with the presence of heavy metals in water sources in Doha, Qatar. Fourier Transform Infrared (FTIR) analysis revealed that the plastic plate and spoon were composed of polyolefin, with the spoon exhibiting additional peaks that indicated oxidation or the presence of additives. Thermogravimetric Analysis (TGA) revealed that the spoon exhibited higher thermal stability, retaining approximately 10% of its mass at 700 °C, than the plate, which retained 2%, indicating the presence of complex additives or contamination. MPs in food-grade salt samples were verified through filtration and Fourier Transform Infrared (FTIR) Spectroscopy, identifying polymers such as polyethylene (PE), polypropylene (PP), and polyethylene terephthalate (PET). These MPs likely stem from exposure to packaging or environmental contaminants. FTIR spectra confirmed the integrity of the polymers after treatment. Inductively Coupled Plasma–Optical Emission Spectroscopy (ICP-OES) analysis revealed varying levels of heavy metals in bottled and tap water, with notable findings including detectable arsenic and lead in both, higher calcium and magnesium in bottled water, and the presence of copper present in tap water only, highlighting potential health and infrastructure-related concerns. These results highlight the possible risks associated with exposure to MPs and heavy metals from everyday products and water sources, underscoring the need for enhanced regulatory oversight and safer material choices to ensure protection. Full article

The mechanisms of ictal discharge initiation remain incompletely understood, particularly the paradoxical role of inhibitory fast-spiking interneurons in seizure generation. Using simultaneous whole-cell recordings of interneurons and pyramidal neurons combined with extracellular [K⁺]_o monitoring in mouse entorhinal cortex-hippocampal slices (4-aminopyridine model of epileptiform activity), we identified a critical transition sequence: interneurons displayed high-frequency firing during the preictal phase before entering depolarization block (DB). DB onset coincided with the peak of rate of extracellular [K⁺] accumulation. Pyramidal cells remained largely silent during interneuronal hyperactivity but started firing within 1.1 ± 0.3 s after DB onset, marking the transition to ictal discharges. This consistent sequence (interneuron DB → [K⁺]_o rate peak → pyramidal cell firing) was observed in 100% of entorhinal cortex recordings. Importantly, while neurons across all entorhinal cortical layers synchronously fired during the first ictal discharge, hippocampal CA1 neurons showed fundamentally different activity: they generated high-frequency interictal bursts but did not participate in ictal events, indicating region-specific seizure initiation mechanisms. Our results demonstrate that interneuron depolarization block acts as a precise temporal switch for ictogenesis and suggest that the combined effect of disinhibition and K⁺-mediated depolarization triggers synchronous pyramidal neuron recruitment. These findings provide a mechanistic framework for seizure initiation in focal epilepsy, highlighting fast-spiking interneurons dysfunction as a potential therapeutic target. Full article

Mental health disorders, particularly depression and anxiety, have become increasingly prevalent among elite athletes, exacerbated by factors such as competitive pressure and the Coronavirus Disease 19 (COVID-19) pandemic. This study analyzes trends in the use of antidepressants, anxiolytics, and cannabinoids (delta-9-tetrahydrocannabinol (THC)/cannabidiol (CBD)) among Italian athletes from 2011 to the first half of 2023 (FH2023), referring to anti-doping reports published by the Italian Ministry of Health. Data from 13,079 athletes were examined, with a focus on non-prohibited medications, banned substances, and regulatory impacts, including threshold adjustments for THC since 2013 and the legalization of CBD. The results show fluctuating use of antidepressants/anxiolytics, with peaks in 2021 and the FH2023, coinciding with post-pandemic awareness. Positive THC cases rose following regulatory changes, reflecting socio-cultural trends. Gender disparities emerged, with THC use predominantly among males (e.g., nine males vs. one female in 2013), though female athletes were underrepresented in testing. This study highlights the need for personalized, evidence-based strategies that balance therapeutic efficacy and anti-doping compliance. Clinicians should carefully consider prescribing selective serotonin reuptake inhibitors (SSRIs) and benzodiazepines to address depression and anxiety and should monitor the risks of CBD contamination. Future research should adopt longitudinal, gender-sensitive approaches to refining guidelines and combating stigma in professional sports. Full article

Background: The COVID-19 pandemic progressed unevenly across the 417 municipalities of Bahia, Brazil. Pinpointing where and when risk peaked is vital for preparing for future emergencies. Methods: We performed an ecological, spatiotemporal study using COVID-19-confirmed cases in Bahia, Brazil, from January 2020 to December 2022. A discrete Poisson space–time scan in SaTScan-identified clusters. For each cluster, we calculated relative risk (RR) and Log Likelihood Ratio, considering p < 0.05 as significant. Results: A total of 33 clusters were detected; 25 statistically significant. The largest cluster (164 municipalities; May 2020–June 2021) comprised 702,720 observed versus 338,822 expected cases (RR = 2.8). Two overlapping large clusters (185 and 136 municipalities) during January–February 2022—coinciding with Omicron circulation—showed RR > 2.0. Localized clusters reached RR > 3.0. Spatially, risk concentrated in the south, southwest, and east of the state, with isolated countryside outbreaks. Conclusions: The heterogeneous spatiotemporal dynamics of COVID-19 in Bahia underscore the value of cluster detection for targeted surveillance and resource allocation. We recommend employing statistical techniques for early detection and control, as well as conducting further studies on socioeconomic and behavioral factors. Full article

Microgrids and load shifting can improve resilience and lower costs for electricity customers. The costs to deploy each have decreased and helped accelerate their deployment in the U.S. and globally. However, previous research has focused minimally on the combined benefit or “stacked economic value” that these assets could provide jointly. This article evaluates the financial value when those assets are combined and optimized jointly. The methods are demonstrated for a U.S. government facility with an existing microgrid and building automation system, with optimizations that vary the percentage load shifted and the duration of time the load can be shifted. The economic benefits of load shifting are greater when combined with a microgrid and coordinated dispatch of loads and microgrid assets. The methods and case study results illustrate “stacked economic value” showing energy charge reductions are 56–252% greater and demand charge reductions are 96–226% greater when load shifting is combined with a microgrid as compared to load shifting without a microgrid. Increasing the amount and duration of load shifting improves the stacked economic value as more loads are scheduled coincident with on-site generation to offset or completely avoid utility purchases during peak pricing periods, an underlying behavior that enables stacked economic value and increased financial savings. The percentage reduction in demand charges is greater than energy charges—a generalizable finding—but the relative impact on utility expenditures is dependent on the utility tariff structure and composition of demand charges and energy charges in the utility bill. In this case study, demand charge reductions were four times greater than energy charge reductions, but the financial savings of demand charges are less due to their smaller proportion of utility charges. This suggests that the stacked economic value of microgrids and load control may be even more significant in locations with electricity tariffs that more heavily weight billing towards demand charges than energy charges. Full article

Floral ontogeny in soybean (Glycine max) is governed by multilayered regulatory hierarchies that integrate phytohormonal cues with precisely choreographed gene-expression programs. Yet, the transcriptomic architecture underpinning this continuum remains only partially resolved. Here, we generated a strand-specific, high-depth temporal transcriptome atlas of soybean inflorescences spanning four morphologically defined stadiums (Stadium 0–Stadium 3). We detected transcriptional activity for 60,889 loci; pairwise stadium contrasts revealed 4000–7000 differentially expressed genes, with the most extensive reprogramming coinciding with the onset of anthesis (Stadium 2). Unsupervised clustering delineated ~600 genes peaking at the pre-anthesis phase (Stadium 1), a cohort enriched for transcriptional regulators and floral organ-identity determinants. Stadium-resolved gene-set enrichment and KEGG mapping uncovered dynamic modulation of canonical hormone-signaling pathways—including auxin, cytokinin, gibberellin, abscisic acid, ethylene, jasmonate, and salicylate circuits—reflecting shifting developmental priorities. Forty-five MADS-box transcription factor genes were expressed; notably, JOINTLESS was strongly induced at anthesis, while the root-predominant factor GmNMH7 exhibited unexpected floral expression, implicating a hitherto unappreciated role in reproductive development. Quantitative RT-PCR of representative loci corroborated RNA-seq measurements. This high-resolution atlas refines our understanding of the hormonal and genetic circuitry of soybean floral morphogenesis, furnishing molecular targets for engineering flowering time and inflorescence architecture under fluctuating environmental conditions. Full article

Respiratory syncytial virus (RSV) is a major etiological agent of lower respiratory tract infections, particularly among infants and the elderly. Activation of B cells in the mucosa and the production of specific neutralizing antibodies are essential for protective immunity against pulmonary infection. B-cell activating factor (BAFF) is a critical survival factor for B cells and has been associated with antiviral responses; however, its regulation during RSV infection remains poorly understood. This study examined BAFF regulation in BEAS-2B cells exposed to RSV or IFN-β. The treatments resulted in a progressive increase in gene expression over time, accompanied by higher protein levels. BAFF mRNA peaked at 12 h post-infection and declined by 48 h, coinciding with the release of soluble BAFF protein into the culture supernatant. Pre-treatment with anti-IFN-β antibodies prior to RSV infection reduced both BAFF mRNA and protein levels, indicating that IFN-β plays a regulatory role in BAFF production by airway epithelial cells. Western blot analysis revealed membrane-bound BAFF (~31 kDa) in non-infected cells, with elevated expression at 24 h post-infection. By 48 h, this form was cleaved into a soluble ~17 kDa form, which was detected in the supernatant. Immunostaining further demonstrated reduced surface expression of membrane-bound BAFF in RSV-infected cells compared to uninfected controls, suggesting that RSV infection promotes the cleavage and release of BAFF into the extracellular environment. Additionally, the release of BAFF was not affected by furin convertase inhibition or ER–Golgi transport blockade, indicating a potentially novel cleavage mechanism. Co-culturing BAFF produced by BEAS-2B cells with isolated B cells enhanced B cell viability. Overall, these results indicate that RSV infection stimulates BAFF production in airway epithelial cells through a pathway involving IFN-β, potentially contributing to B cell activation and promoting local antibody-mediated immunity. Understanding this mechanism may offer valuable insights for improving mucosal vaccine strategies and enhancing immunity against respiratory pathogens. Full article

Background/Objectives: Type 2 diabetes mellitus (T2DM) and Parkinson’s disease (PD) are two highly prevalent chronic conditions that often coexist in older adults. Their interaction may influence clinical outcomes, particularly during external stressors such as the COVID-19 pandemic. This study aimed to assess the prevalence and temporal trends of PD among hospitalized patients with T2DM in Spain (2017–2023), evaluate sex-based differences in clinical characteristics and outcomes, examine the impact of the COVID-19 pandemic, and identify predictors of PD diagnosis and in-hospital mortality (IHM). Methods: We conducted a retrospective, nationwide study using the Spanish National Hospital Discharge Database (RAE-CMBD). Adults aged ≥40 years hospitalized with T2DM were included. PD cases were identified using ICD-10 codes. Joinpoint regression assessed temporal trends, and multivariable logistic regression identified factors associated with PD and IHM. Results: Among 5.1 million T2DM-related hospitalizations, 107,931 (2.41%) involved PD. PD prevalence increased over time, particularly among women. Men accounted for most PD cases and were younger than their female counterparts. Depression and anxiety were more frequent in women and associated with PD in both sexes. IHM peaked at 14.6% in 2020, coinciding with the COVID-19 outbreak. Predictors of IHM included older age, higher comorbidity burden, dementia, and COVID-19 diagnosis. Conclusions: The coexistence of PD and T2DM in hospitalized patients is associated with clinical complexity and increased mortality. Personalized, multidisciplinary care is essential to address sex-specific patterns, psychiatric comorbidities, and vulnerability to systemic stressors. Full article

The study examined seasonal variability in soil enzymatic activity and microbial abundance across five grassland vegetation units: Molinietum caeruleae, Alopecuretum pratensis, Arrhenatheretum elatioris, Lolio–Cynosuretum, and com. Poa pratensis–Festuca rubra. Soils under Molinietum caeruleae showed higher fungal abundance and greater plant diversity, while Lolio–Cynosuretum was notable for elevated Azotobacter spp. populations. Actinobacteria preferred soils with more organic matter, whereas Azotobacter spp. favored higher pH. A negative correlation was observed between the Shannon diversity index (H′) and heterotrophic bacterial abundance in Arrhenatheretum elatioris and with fungal abundance in com. Poa pratensis–Festuca rubra. Acid and alkaline phosphatase and catalase activities were also negatively correlated with H′. Redundancy analysis showed these enzymes were related to total nitrogen content, and enzyme activity decreased with rising soil pH. In autumn 2022, high fungal abundance coincided with a reduction in other microorganisms. Seasonal trends were evident: catalase and urease activities peaked in autumn 2023, while other enzymes were more active in spring 2022. The results emphasize the significance of seasonal shifts in shaping microbial and enzymatic soil processes, which are vital for nutrient cycling, carbon sequestration, and climate regulation. Further research is essential to guide sustainable grassland soil management. Full article