Search Results (93)

Specialty coffee commercialization has experienced a consistent upward trend over the past several years. The prevalence of specialty coffee consumption has increased considerably, particularly among younger demographics and people who engage in physical activities. Sellers are actively involved in the development of innovative formulas and modifications to maintain the competitiveness of their product in the market. Here, we propose a naturally infused caffeine drink with cascara extract as an alternative social beverage. This beverage was formulated using extracts derived from Arabica Ethiopia coffee beans and coffee cherry shells. The final cascara-infused caffeine drink comprises a 50% Ethiopian Arabica coffee infusion and a 50% coffee cherry shell infusion. This beverage is characterized by an average caffeine content of 0.28 mg/mL, a caffeic acid content of 0.24 mg/mL, and a chlorogenic acid content of 0.13 mg/mL. Furthermore, 100 mL of the cascara-infused coffee drink is enriched with polyphenol compounds at an amount of 80.6 mg of Gallic Acid Equivalents per liter (mg GAE/L), including 67.6 mg of catechin equivalent per liter (mg CAE/L) flavonoids. The formulation of the infused caffeine drink contains natural sugars such as glucose, sucrose, and fructose, in amounts of 0.17 mg/mL, 0.97 mg/mL, and 1.66 mg/mL, respectively. The developed procedure has the potential to enhance the coffee-sale market. Full article

Hydrothermal processing has emerged as a promising clean technology for managing the substantial amounts of agro-industrial waste generated worldwide. This study aims to introduce a clean technology approach to biomass valorization processes by exploring the hydrothermal conversion of two distinct biomass feedstocks, peapods and coffee cherries, into valuable platform chemicals through the use of homogeneous acid catalysts. The hydrothermal valorization experiments were conducted in a 500 mL reactor at 180 °C for 1 h with a 1:20 biomass–acid solution ratio, utilizing a set of organic and inorganic acids as catalysts. The chemical compositions of the biomass feedstocks were analyzed, revealing significant differences in their cellulose (20.2 wt% in peapods; 27.6 wt% in coffee cherries), hemicellulose (17.4 wt% in peapods; 12.5 wt% in coffee cherries), and lignin (5.0 wt% in peapods; 13.7 wt% in coffee cherries) contents. Without the use of catalysts, peapods yielded 45.128 wt% platform chemicals, outperforming coffee cherries, which produced 32.598 wt%. The introduction of various acid catalysts influenced the yields and selectivity of platform chemicals. Sulfuric acid enhanced sugar production, yielding 62.936 wt% from peapods and 51.236 wt% from coffee cherries. Hydrochloric acid selectively favored sugar production but resulted in decreased overall yields. Nitric acid facilitated the conversion of both biomass types, yielding 35.223 wt% from coffee cherries and 40.315 wt% from peapods. Adipic acid achieved the highest overall yields, with 53.668 wt% for coffee cherries and 65.165 wt% for peapods, while also increasing levulinic acid production. Acetic acid significantly increased sugar yields, which reached 50.427 wt% with peapods. The findings highlight the potential of hydrothermal valorization as a clean technology for biomass conversion and underscores the importance of tailoring catalyst selection and process conditions to optimize the valorization of biomass feedstocks. Full article

Coffee and coffee by-products, such as coffee cherries, coffee flowers, coffee leaves, green beans, roasted coffee, instant coffee, spent coffee grounds, and silverskin, contain a complex mixture of bioactive compounds that may exhibit both genotoxic and antimutagenic effects. This article evaluates in vitro studies on the genotoxic potential of coffee and coffee by-products, with a focus on different preparation methods, roasting processes, and key chemical constituents. Furthermore, given the growing interest in utilizing coffee by-products for novel food applications, this review sought to identify knowledge gaps regarding their safety. The impact of metabolic activation, particularly the role of enzymatic detoxification and bioactivation, was examined to better understand the effects on genetic material. The findings suggest that while certain compounds in coffee can induce DNA damage under specific conditions, the overall evidence does not indicate a significant genotoxic risk to consumers. However, further studies, particularly in vivo and human studies, appear necessary to ensure the requirements of novel food applications for some coffee by-products. Full article

In order to explore the optimal vibration parameters for the selective harvesting of coffee fruits, a high-velocity dynamic photography monitoring system was developed to analyze the vibration-assisted harvesting process. This system identified the optimal vibration position on coffee branches and facilitated theoretical energy transfer analysis, obtaining a mathematical formula for calculating the total kinetic energy of coffee branches. A single-factor experiment was conducted with the vibration position as the experimental factor and the total kinetic energy of coffee branches as the response variable. The results showed that the total kinetic energy of the branches was the highest at Vibration Position 2 (the position between the third and the fourth Y-shaped bud tips on the branch). Therefore, Vibration Position 2 was determined as the optimal vibration position. Further analysis established a mathematical model linking coffee cherry motion parameters to theoretical detachment force. A factorial experiment was conducted with vibration frequency and amplitude as test factors, using detachment rates of green, semi-ripe, and ripe cherries as indicators. The results showed that at 55 Hz and 10.10 mm amplitude, the detachment rate of ripe cherries was highest (83.33%), while green and semi-ripe cherries detached at 16.67% and 33.33%, respectively. A field validation experiment, with Vibration Position 2, 55 Hz frequency, 10.10 mm amplitude, and 1 s vibration duration, yielded actual detachment rates of 15.86%, 35.17%, and 89.50% for green, semi-ripe, and ripe cherries, respectively. The error margins compared with the theoretical values were all below 10%. These results confirm the feasibility of optimizing vibration harvesting parameters through high-velocity photography dynamic analysis. Full article

Effective grinding of residual agricultural materials helps to improve yield in the production of chemical compounds through hydrothermal technology. Milling pretreatment has different types of pre-treatment where ball mills, roller mills, and finally, the knife mill stand out. The knife mill being a mill with continuous processing, its multiple benefits and contributions highlight the knife milling process; however, it is a process that is generally carried out with dry biomass that generates extra processing of the biomass before grinding, implying longer times and wear than other equipment. This work presents the design of a knife mill with an adaptation of free convection drying as a joint process of knife milling and drying. The design is based on lignocellulosic biomass, and the knife milling results are presented for two biomasses: peapods and coffee cherries. The knife mill is designed with a motor, a housing with an integrated drive system, followed by a knife system and a feeding system with a housing and finally the free convection drying system achieving particle sizes in these biomasses smaller than 30 mm, depending on the time processed. The data demonstrate the significant impact of particle size on the yields of various platform chemicals obtained from coffee cherry and peapod waste biomass. For coffee cherry biomass, smaller particle sizes, especially 0.5 mm, result in higher total yields compared to larger sizes while for peapod biomass at the smallest particle size of 0.5 mm, the total yield is the highest, at 45.13%, with notable contributions from sugar (15.63%) and formic acid (19.14%). Full article

Coffee production is a vital source of income for smallholder farmers in Mexico’s Chiapas, Oaxaca, Puebla, and Veracruz regions. However, climate change, fluctuating yields, and the lack of decision-support tools pose challenges to the implementation of sustainable agricultural practices. The SABERES project aims to address these challenges through a Seq2Seq-LSTM model for predicting coffee yields in the short term, using datasets from Mexican national institutions, including the Agricultural Census (SIAP) and environmental data from the National Water Commission (CONAGUA). The model has demonstrated high accuracy in replicating historical yields for Chiapas and can forecast yields for the next two years. As a first step, we assessed coffee yield prediction for Bali, Indonesia, by comparing the LSTM, ARIMA, and Seq2Seq-LSTM models using historical data. The results show that the Seq2Seq-LSTM model provided the most accurate predictions, outperforming LSTM and ARIMA. Optimal performance was achieved using the maximum data sequence. Building on these findings, we aimed to apply the best configuration to forecast coffee yields in Chiapas, Mexico. The Seq2Seq-LSTM model achieved an average difference of only 0.000247, indicating near-perfect accuracy. It, therefore, demonstrated high accuracy in replicating historical yields for Chiapas, providing confidence for the next two years’ predictions. These results highlight the potential of Seq2Seq-LSTM to improve yield forecasts, support decision making, and enhance resilience in coffee production under climate change. Full article

Submerged fermentation offers a controlled environment for coffee processing, ensuring a consistent temperature and aerobic–anaerobic conditions, making it a superior alternative to solid-state fermentation. This study aimed to optimize submerged fermentation conditions for green coffee beans to maximize total phenolic content (TPC) and antioxidant activity, such as ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), DPPH (2,2-Diphenyl-1-picrylhydrazyl), and FRAP (the ferric reducing antioxidant power). Additionally, pH, yeast, and lactic acid bacteria counts were monitored. Fermentation was conducted with selective microbial starters, a varying temperature (25–35 °C), incubation time (3–9 days), and coffee weight (5–10 g) using a Box–Behnken design. To enhance bioactive compound infusion, fresh coffee cherries underwent ultrasonic treatment, increasing their porosity and water-holding capacity. Vacuum impregnation was then used to infuse fermented green coffee bean extract into the cherries. The lowest pH coincided with peak yeast growth, while the coffee weight significantly influenced all responses. The incubation time affected most parameters except DPPH activity, and the temperature impacted only ABTS and DPPH activities. Optimal conditions (35 °C; 7.21 days; 10 g) yielded a TPC of 480.25 µmol GAE/100 g with ABTS, DPPH, and FRAP activities of 725.71, 164.15 and 443.60 µmol TE/g, respectively. Ultrasound-treated coffee cherries exhibited increased porosity and absorption capacity, facilitating enhanced bioactive compound infusion during 3 h of vacuum impregnation. In conclusion, submerged fermentation effectively improves bioactive compound production, while ultrasound treatment and vacuum impregnation present promising methods for developing high-value dehydrated coffee cherry products. Full article

Coffee pulp, which accounts for approximately 40% of the dry weight of coffee cherries, is one of the many byproducts produced by the world’s most popular beverage, coffee. Such neglected waste represents an interesting source of bioactive compounds, such as procyanidins, which have antioxidant, anti-inflammatory, and neuroprotective properties. This study aims to develop an efficient method for procyanidins extraction from Coffea arabica pulp using a novel microwave–ultrasound hybrid method of extraction. Microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE) and a novel hybrid method (MAE–UAE) were comparatively analyzed. Using Box–Behnken design, the hybrid extraction method was optimized, giving a procyanidin yield of 60.88 mg/g (under these conditions: ~60 °C, ~21 min, ~1:13 solid-to-liquid ratio). The purification was carried out through a Sephadex LH-20 packed column chromatography, and the identified procyanidin dimers and trimers were confirmed by HPLC/ESI-MS. The hybrid extract’s acetonic fraction’s DPPH and ABTS tests revealed that procyanidins had a greater capacity to scavenge radicals than Trolox (p < 0.05). The findings highlight the potential of sustainable extraction methods for valorizing coffee pulp in functional food and pharmaceutical applications. Full article

Background/Objectives: This study aimed to develop and evaluate an anti-pollution film-forming spray (FFS) containing coffee cherry pulp extract (FFS-CCS). The formulation was designed to create a protective skin barrier, improving skin health while defending against environmental pollutants. Its physical properties, dust resistance, stability, skin penetration, and clinical effectiveness were assessed to ensure optimal performance and safety. Methods: Various polymers and a ternary solvent system were used to enhance the stability and solubility of bioactive compounds from the coffee cherry pulp extract. The formulations were characterized based on appearance, film formation, viscosity, pH, spray uniformity, spray pattern, angle, film thickness, and particle adhesion. Stability testing was conducted under different storage conditions. Skin penetration was assessed using Franz diffusion cells with Strat-M^® membranes to simulate human skin. A single-blind, placebo-controlled trial with 42 participants was conducted over 60 days to evaluate the effects of FFS-CCS on skin hydration, tone, and wrinkle reduction. Clinical assessments were performed using a Corneometer, Mexameter, and Skin Visioscan. Results: The FFS1-CCS formulation, incorporating PVP K90 and a ternary solvent system, significantly improved the solubility, stability, and bioavailability of key bioactive compounds (chlorogenic acid, caffeine, and theophylline). Physical characterization confirmed uniform, transparent films with optimal viscosity and sprayability. Stability testing showed minimal degradation. Skin penetration and retention studies revealed enhanced retention of bioactive compounds with minimal systemic absorption. PVP K90, along with ethanol and propylene glycol, extended the compounds’ residence time on the skin, ensuring localized delivery. Clinically, FFS1-CCS significantly improved skin hydration, reduced roughness, lightened skin tone, and decreased erythema. Conclusions: The FFS1-CCS formulation utilizing PVP K90 significantly enhanced the stability, bioavailability, and skin retention of coffee cherry pulp extract, resulting in improved skin hydration, wrinkle reduction, and skin tone enhancement. These findings highlight the potential of coffee cherry pulp extract as a multifunctional, sustainable cosmeceutical ingredient, offering both anti-aging and environmental protection benefits, making it a promising solution for skincare applications. Full article

Aspergillus carbonarius is the main producer of Ochratoxin A (OTA) in coffee. In the last few years, there has been an increasing interest in using yeast isolates as Biocontrol Agents to prevent OTA production in coffee cherries during the primary postharvest processing. Little is known about how climate change abiotic conditions of increased temperature (+2–4 °C), elevated CO₂ (existing levels of 400 vs. 1000 ppm), and increased drought stress will impact biocontrol resilience. This study examined the effect of a three-way interaction between temperature (27, 30, and 33 °C) x water activity (a_w) (0.90 and 0.95 a_w) x CO₂ level (400 vs. 1000 ppm) on the growth and OTA production of A. carbonarius and the resilience of three yeast strains’ biocontrol capacity on fresh coffee cherries. High a_w (0.95), CO₂, and temperature levels increased the production of OTA by A. carbonarius. All the yeast biocontrol strains significantly reduced A. carbonarius growth by at least 20% and OTA production by up to 85%. From the three strains used, the Meyerozyma caribbica strain (Y4) showed the best resilience to climate change, since it reduced both growth (50%) and OTA production (70%) under future scenarios of CO₂ and a_w at all temperatures tested, and should be the one selected for pilot scale experiments in Ivory Coast. Full article

Coffee cherry waste, a byproduct of coffee production, presents significant environmental challenges due to its large volume—approximately 20 million tons annually. The disposal of this waste, which includes pulp and mucilage, often leads to pollution of land and water systems, contributing to environmental degradation. Additionally, the high acidity and organic content of coffee cherry waste complicate its management, making it crucial to find sustainable solutions for its valorization and reuse in order to mitigate these ecological impacts. The purpose of this study is to investigate the efficiency and selectivity of various organic acid catalysts in the hydrothermal valorization of coffee cherry waste. The procedures were conducted using the liquid hot water (LHW) treatment for one hour with a 1:20 biomass/catalyst ratio and a 1 mm biomass particle size at 180 °C in 500 mL batch reactors modifying 10 different organic acids at 0.02 M. Concentrations of the valorized products (HMF, furfural, levulinic acid, formic acid, and sugars) were measured using HPLC-IR. Among the catalysts tested, adipic acid demonstrated the highest efficiency, with a total yield of 53.667%, showing significant selectivity towards formic acid (19.663%) and levulinic acid (11.291%). In contrast, butyric acid was the least efficient catalyst, yielding a total of 17.395% and showing minimal selectivity towards other compounds. Chloroacetic acid and benzoic acid were notable for their high selectivity towards sugars. Other catalysts, such as anthranilic acid, propanoic acid, and succinic acid, displayed moderate efficiency and selectivity, with balanced yields across various compounds. These findings highlight the importance of catalyst selection in optimizing the hydrothermal process for desired product outcomes. Full article

Arabica coffee, one of the most valuable crop commodities, harbors diverse microbial communities with unique genetic and functional traits that influence bean safety and final coffee quality. In Ecuador, coffee production faces challenges due to the spread of pathogenic organisms across cultivars, leading to reduced yields and compromised quality. This study employed a shotgun metagenomic approach to characterize the indigenous microbial diversity present in the cell biomass of fermented coffee cherries from three Coffea arabica varieties: Typica (Group A), Yellow Caturra (Group B), and Red Caturra (Group C), originating from the Intag Valley in northern Ecuador, at two ripe stages: green (immature fruits) and ripe (red/yellow mature fruits). Gene prediction and functional annotation were performed using multiple databases, including EggNOG, COG, KEGG, CAZy, CARD, and BacMet, to explore the potential impact of microbial communities on bean quality and safety. Metagenomic sequencing generated over 416 million high-quality reads, averaging 66 million clean reads per sample and yielding a total of 47 Gbps of data. Analysis revealed distinct differences in species abundance based on the coffee variety and ripening stage. A total of 799,658 protein-coding sequences (CDSs) were predicted, of which 205,937 genes were annotated with EggNOG, 181,723 with COG, 155,220 with KEGG, and 10,473 with CAZy. Additionally, 432 antibiotic resistance genes (ARGs) were identified using CARD, and 8974 biocide and metal resistance genes (BMRGs) were annotated with BacMet. Immature cherries exhibited enriched pathways associated with resistance to antibiotics such as fluoroquinolones, penams, rifamycin, macrolides, carbapenems, and cephalosporins. The abundance of these pathways varied with the ripening stage and variety. Furthermore, green cherries showed a significant increase in BMRGs associated with resistance to substances including hydrochloric acid, copper, nickel, hydrogen peroxide, arsenic, and zinc. Among mature cherries, Typica and Red Caturra shared similar profiles, while Yellow Caturra displayed a divergent microbial and functional profile. These study findings emphasize the interplay between microbial diversity, ripening stages, and coffee varieties, providing a foundation for innovative approaches to enhance coffee quality through microbiome management. Full article

This study presents the design, fabrication, and performance evaluation of a batch reactor for the hydrothermal valorization of coffee and peapod waste biomass. The reactor, designed using Inventor 2023 and analyzed using ANSYS 2023, is capable of operating at elevated temperatures to facilitate the breakdown of lignocellulosic structures and promote the extraction of valuable platform chemicals. Based on the design, the reactor was manufactured, and a set of experiments was performed to test it at different temperatures ranging from 120 to 180 °C, at different times (1–4 h) and with different types of biomass (peapods and coffee cherry waste). The results demonstrate the effectiveness of the reactor in optimizing the conversion of agricultural waste into key compounds such as hydroxymethylfurfural (HMF) and furfural. For peapod biomass, optimal conditions were identified at temperatures between 150 °C and 180 °C, with a reaction time of approximately 1 h yielding up to 72.17%wt total platform chemicals. Coffee cherry biomass showed a different yield profile, with total platform chemical yields reaching 23.56%wt at 180 °C after 4 h of treatment, highlighting the importance of feedstock-specific optimization. The reactor’s performance indicates its potential for broader applications in the conversion of various lignocellulosic feedstocks into high-value chemicals, contributing to a more sustainable and circular economy. The presented design and analysis demonstrate the reactor’s compliance with necessary characteristics such as temperature, deformation, and stress, ensuring its suitability for the experiments. This study showcases the importance of innovative reactor design in advancing sustainable chemical production from biomass waste, making it a valuable contribution to the field of waste-to-value conversion technologies. Full article

Spent coffee grounds generated from the brewing of coffee cherries are rich in chlorogenic acids that are associated, to a certain extent, with a delay in the development of various chronic diseases and age-related disorders. These natural antioxidants are applied in the pharmaceutical, cosmetic, and food industries. This brief overview describes recently proposed procedures for the extraction and recovery of chlorogenic acids from spent coffee grounds, which is a low-cost and easily accessible by-product. Solvent selection and temperature control seem to be the main factors due to the thermolabile nature of these compounds. Advanced extraction technologies are generally faster and enhance extraction efficiency. Procedures for the valorization of coffee waste are the goal of a sustainable and circular bioeconomy that seeks to increase their added benefits and reduce environmental pollution. Full article

Coffee and cocoa agribusinesses generate large volumes of byproducts, including coffee husk, coffee pulp, parchment skin, silver skin, and cocoa bean shell. Despite the rich composition of these materials, studies on biomolecule extraction with green solvents are still scarce, and further research is needed. Extraction methods using alternative solvents to obtain biomolecules must be developed to enhance the byproducts’ value and align with biorefinery concepts. This article reviews the compositions of coffee and cocoa byproducts, their potential applications, and biomolecule extraction methods, focusing on alternative solvents. The extraction methods currently studied include microwave-assisted, ultrasound-assisted, pulsed electric field-assisted, supercritical fluid, and pressurized liquid extraction. At the same time, the alternative solvents encompass the biobased ones, supercritical fluids, supramolecular, ionic liquids, and eutectic solvents. Considering the biomolecule caffeine, using alternative solvents such as pressurized ethanol, supercritical carbon dioxide, ionic liquids, and supramolecular solvents resulted in extraction yields of 2.5 to 3.3, 4.7, 5.1, and 1.1 times higher than conventional solvents. Similarly, natural deep eutectic solvents led to a chlorogenic acid extraction yield 84 times higher than water. The results of this research provide a basis for the development of environmentally friendly and efficient biomolecule extraction methods, improving the utilization of agricultural waste. Full article