Search Results (324)

Fungi, from saprophytes to pathogens, face predictable daily fluctuations in light, temperature, humidity, and nutrient availability. To cope, they have evolved an internal circadian clock that confers a major adaptive advantage. This review critically synthesizes current knowledge on the molecular architecture and physiological relevance of fungal circadian systems, moving beyond the canonical Neurospora crassa model to explore the broader phylogenetic diversity of timekeeping mechanisms. We examine the core transcription-translation feedback loop (TTFL) centered on the FREQUENCY/WHITE COLLAR (FRQ/WCC) system and contrast it with divergent and non-canonical oscillators, including the metabolic rhythms of yeasts and the universally conserved peroxiredoxin (PRX) oxidation cycles. A central theme is the clock’s role in gating cellular defenses against oxidative, osmotic, and nutritional stress, enabling fungi to anticipate and withstand environmental insults through proactive regulation. We provide a detailed analysis of chrono-pathogenesis, where the circadian control of virulence factors aligns fungal attacks with windows of host vulnerability, with a focus on experimental evidence from pathogens like Botrytis cinerea, Fusarium oxysporum, and Magnaporthe oryzae. The review explores the downstream pathways—including transcriptional cascades, post-translational modifications, and epigenetic regulation—that translate temporal signals into physiological outputs such as developmental rhythms in conidiation and hyphal branching. Finally, we highlight critical knowledge gaps, particularly in understudied phyla like Basidiomycota, and discuss future research directions. This includes the exploration of novel clock architectures and the emerging, though speculative, hypothesis of “chrono-therapeutics”—interventions designed to disrupt fungal clocks—as a forward-looking concept for managing fungal infections. Full article

Cuscohygrine (CUS) and hygrine (HYG) are pyrrolidine alkaloids proposed as biomarkers of coca leaf consumption, a culturally accepted practice in some Latin American countries. Differentiating legal coca use from illicit cocaine consumption holds forensic importance. While LC-MS/MS is preferred, GC-MS remains widely used in Latin American toxicology labs due to accessibility. This study critically evaluates the analytical limitations of GC-MS for detecting CUS and HYG in biological matrices. Key parameters—injector temperature (180–290 °C), injection mode (split/splitless), solvent, liner condition, and matrix—were systematically studied. GC-MS showed significant limitations: low-abundance, non-specific fragments (m/z 42, 84, 98, 140) failed to meet the identification criteria in SIM mode. Thermal degradation of CUS to HYG and CUS-d6 to HYG-d3 was observed, especially with splitless injection and aged liners. Matrix effects produced signal enhancement ranging from +29% to +316%, meaning that analyte responses in biological samples were significantly higher than in neat standards, likely due to reduced degradation or adsorption. Although deuterated internal standards (CUS-d6) partially corrected signal variability and matrix enhancement, these corrections were not sufficient to overcome the fundamental limitations of GC-MS, including poor ion specificity and compound instability. These findings support the need for LC-MS/MS-based approaches for reliable alkaloid detection and question the suitability of GC-MS for CUS analysis in forensic toxicology contexts. Full article

Precipitation-hardenable stainless steels (PHSS) are widely used in various applications in the aeronautical industry such in as landing gear supports, actuators, and fasteners, among others. This research aims to study the pitting corrosion behavior of passivated martensitic precipitation-hardening stainless steel, which underwent passivation for 120 min at 25 °C and 50 °C in citric and nitric acid baths before being immersed in solutions containing 1 wt.% sulfuric acid (H₂SO₄) and 5 wt.% sodium chloride (NaCl). Electrochemical characterization was realized employing electrochemical noise (EN), while microstructural analysis employed scanning electron microscopy (SEM). The result indicates that EN reflects localized pitting corrosion mechanisms. Samples exposed to H₂SO₄ revealed activation–passivation behavior, whereas those immersed in NaCl exhibited pseudo-passivation, indicative of an unstable oxide film. Current densities in both solutions ranged from 10⁻³ to 10⁻⁵ mA/cm², confirming susceptibility to localized pitting corrosion in all test conditions. The susceptibility to localized attack is associated with the generation of secondary oxides on the surface. Full article

This study investigates the valorization of carrot discard, a carbohydrate-rich agricultural residue, for the production of 2,3-butanediol (2,3-BDO). The fermentation process was evaluated using two strains, Bacillus licheniformis DSM 8785 and Bacillus amyloliquefaciens DSM 7. Two process configurations were compared: separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF). Additionally, to determine substrate and product inhibition thresholds, fermentation assays were conducted in semi-defined media with glucose concentrations ranging from 20 to 120 g/L. The SHF strategy proved more effective than the SSF configuration. Under the SHF configuration, B. amyloliquefaciens demonstrated superior performance, yielding 16.7 g/L of 2,3-BDO. In contrast, B. licheniformis was notable for its high capacity for acetoin synthesis, producing 24.2 g/L of acetoin in addition to 10.9 g/L of 2,3-BDO. Therefore, these findings demonstrate that carrot discard is a viable feedstock for the co-production of 2,3-BDO and acetoin. Full article

The present review summarizes the existing knowledge regarding the afforestation of sand dunes. Our main focus was on the role of trees in stabilizing and rehabilitating these complex ecosystems. We analyzed 937 publications through a systematic bibliometric review and then proceeded to select 422 articles that met our criteria. This methodological approach—combining a comprehensive bibliometric analysis with an in-depth traditional literature review—represents a novel contribution to the field and allows for both quantitative trends and qualitative insights to be captured. This was then complemented by an in-depth literature review. Our results sustain the global importance of this subject, as they include studies from more than 80 countries, with a focus on the USA, China, Australia, and Japan. We have also identified a series of main tree species that are usually used in the afforestation of sand dunes (Pinus, Acacia, Juniperus) and then proceeded to analyze their ecologic and socio-economic impact. As such, we have analyzed case studies from all continents, showcasing a variety of strategies that were successful and adapted to local conditions. This did not exclude challenges, mainly invasive species, low survival rates, and effects on biodiversity and stabilization. The main factors that impact the success of afforestation are represented by topography, soil structure, water dynamics, and climate. Unlike previous reviews, this study offers a global synthesis of both the scientific output and the applied outcomes of sand dune afforestation, bridging the gap between research and practice. As such, afforestation has a positive impact on soil fertility and carbon sequestration but can also present a major risk to native ecosystems. In this context, the present review highlights the need to adopt strategies that are unique for that site, and that must integrate all aspects (ecological, social, economic) to ensure good results. Our ISI-indexed literature review helped us to address the link between the current knowledge, research trends, and future topics that must be addressed. Full article

Ornithine decarboxylase (ODC) is the rate-limiting enzyme in the biosynthesis of polyamines and plant alkaloids, including medicinal tropane alkaloids (TAs). Due to its key role, ODC has been utilized as an effective molecular tool in metabolic engineering. However, to date, only a limited number of plant ODCs have been characterized. Among the reported ODCs, Erythroxylum coca ODC (EcODC) exclusively has ODC activity, while Nicotiana glutinosa ODC (NgODC) exhibits dual ODC and lysine decarboxylase (LDC) activities. The potential LDC activity of ODCs from TA-producing plants remains unknown. Here, we characterized AlODC and DsODC from Anisodus luridus and Datura stramonium, along with two previously reported ODCs from Atropa belladonna (AbODC) and Hyoscyamus niger (HnODC), in Escherichia coli to investigate their enzyme kinetics and substrate specificity. Enzymatic assays revealed that both AlODC and DsODC catalyzed the conversion of ornithine to putrescine, confirming their ODC activity, with AlODC exhibiting a higher catalytic efficiency, comparable to established ODCs. Furthermore, all four ODCs also displayed LDC activity, albeit at significantly lower efficiency (<1% of ODC activity). This study provides a comprehensive analysis of the enzyme kinetics of ODCs from TA-producing plants, identifying promising candidate genes for metabolic engineering for the biomanufacturing of putrescine-derived alkaloids. Moreover, this is the first report of LDC activity in ODCs from Solanaceae TA-producing plants, shedding light on the evolutionary relationship between ODC and LDC. Full article

This study explores a series of eco-compatible, safe, inexpensive, and recyclable catalysts for the aza-Michael reaction, an essential transformation for constructing C-N bonds. In particular, we focus on hydrothermal carbons (HCB and HCC) prepared from chestnut cupule waste under mild, aqueous conditions, offering a sustainable alternative to traditional pyrolytic methods. These carbonaceous solids, thoroughly characterized by physicochemical techniques, exhibit notable catalytic activity, completing aza-Michael reactions in as little as 5–30 min for various model substrates. Their performance was benchmarked against Montmorillonite K10, [Cho][Pro] ionic liquid, and K10+[Cho][Pro], with the latter combination and [Cho][Pro] alone giving the fastest conversions. For example, the reaction of benzylamine with acrylonitrile reached complete conversion (typically 95% yield) in five minutes using [Cho][Pro], K10+[Cho][Pro], or likewise with HCB and HCC. Although the reactions catalyzed by hydrothermal carbons did not outperform in general those using K10-[Cho][Pro] or [Cho][Pro], they proceeded rapidly and afforded good to excellent yields. Furthermore, the HCC catalyst demonstrated excellent recyclability, maintaining its activity and yield over at least five cycles. These findings underscore the potential of hydrothermal carbons as efficient green heterogeneous catalysts, combining high surface area, porosity, and reusability with strong catalytic performance and scalability, in alignment with the principles of the circular economy. Full article

This study introduces CO₂ laser surface vitrification as an innovative method for managing copper mining tailings, offering a sustainable solution to critical challenges in mineral processing. This technique transforms tailings into a stable and impermeable layer, immobilizing hazardous metals contained within them. By achieving vitrification at the surface level and operating at temperatures around 1200 °C, the process significantly reduces energy consumption compared to traditional vitrification methods, making it suitable for large-scale applications in remote mining sites. Detailed geochemical and mechanical analyses confirmed the formation of a dense vitreous matrix with high hardness (7.19–7.48 GPa) and reduced permeability, ensuring compliance with stringent environmental regulations. However, the brittle nature of the vitrified layer underscores the need for further research to enhance mechanical resilience. This work positions CO₂ laser vitrification as a transformative approach for integrating energy-efficient technologies into mineral processing, addressing key environmental concerns while advancing the sustainable management of mining waste. Full article

Fungi are a diverse and essential group that play crucial ecological roles. However, they remain understudied in tropical countries like Ecuador in terms of their forest or protected areas, particularly across diverse ecosystem zones such as seasonal forests and salt flats. This study aimed to inventory fungal diversity in two specific zones: the dry forest (DF) and the salt flat (SF) within the Reserva Ecologica Arenillas (REAR), located in El Oro, Ecuador. The results recorded 162 specimens representing 47 species belonging to 34 genera, identified morphologically. Although statistically significant, the difference in species richness and abundance between the dry forest and the salt flat was minimal, with the dry forest showing slightly higher values. Nonetheless, certain species were prevalent in both ecosystems, such as Cerrena hydnoides, Pycnoporus sanguineus, Hexagonia tenuis, and Chondrostereum sp., alongside four species with resupinate habit, all of them growing on decayed wood. The Shannon and Simpson indices were calculated to assess alpha diversity, revealing higher diversity in the DF. To evaluate differences in community composition between habitats, non-metric multidimensional scaling (NMDS) and permutational analysis of variance (PERMANOVA) were applied, indicating greater species turnover and dominance of specific taxa in the DF compared to the SF. These findings highlight the importance of the fungal diversity found in the REAR while also pointing to the need for more exhaustive monitoring and comparative studies with other wild or protected areas to fully understand and conserve this biodiversity. Full article

Background/Objectives: Composite dental restorations are continuously exposed to dietary substances, which may compromise their structural integrity. This study aimed to assess the chemical and mechanical effects of coffee, red wine, and Coca-Cola on two widely used commercial resin composites, Herculite Ultra XRV and Omnichroma. Methods: Forty disk-shaped specimens (20 per material) were immersed for 10 days in the selected beverages. Changes in chemical composition were analyzed using Fourier Transform Infrared (FTIR) and Raman spectroscopy, while Vickers microhardness testing evaluated surface hardness. Results: FTIR and Raman analyses revealed that coffee and red wine caused the most substantial chemical degradation, particularly in carbonyl (C=O), aromatic (C=C), and siloxane (Si–O–Si) groups. Herculite XRV demonstrated higher chemical stability, while Omnichroma showed more pronounced molecular degradation. In contrast, microhardness testing indicated that Omnichroma maintained better surface hardness compared to Herculite XRV after exposure. Across all solutions, Coca-Cola induced the least effect. Conclusions: The tested beverages significantly affected both the chemical and mechanical properties of the resin composites. Omnichroma exhibited superior mechanical durability, while Herculite XRV showed greater resistance to chemical degradation. These results highlight the importance of material composition in restorative dentistry and support the development and selection of composites with improved resistance to acidic and staining agents to ensure long-term clinical performance. Full article

This article reports the case of a patient with a gastric neoplasm and total gastrectomy, presenting with severe digestive intolerance, who developed peritoneal and pulmonary tuberculosis. Standard treatment could not be administered. Therefore, treatment was initiated with isoniazid and rifampicin suppositories, and intravenous levofloxacin and amikacin, with significant remission of the digestive symptomatology. Although treatment with rifampicin suppositories has demonstrated efficacy in tuberculosis, it is rarely used in practice. This case highlights the importance of individualizing tuberculosis treatment and demonstrates that rectal administration of isoniazid and rifampicin suppositories, combined with intravenous levofloxacin and amikacin, was successfully used to treat tuberculosis in a patient with severe digestive intolerance, highlighting a potential alternative regimen when standard oral therapy is not feasible. Full article

Cardiovascular (CV) diseases remain a leading global health challenge, being influenced by diet and systemic inflammation. Adherence to healthy dietary patterns, such as the Mediterranean (MED), Dietary Approaches to Stop Hypertension (DASH), and Anti-inflammatory (AnMED) diets, may reduce the CV risk. Background/Objectives: We aimed to evaluate the association between the adherence to healthy dietary patterns and CV treatments. Methods: This cross-sectional study was conducted in the Valencian Community, Spain. Nutritional data were collected using a food frequency questionnaire to assess the adherence to MED, DASH, and AnMED dietary patterns. Statistical analyses, including Kruskal–Wallis tests and linear regression models, evaluated dietary adherence, nutrient intake, the Dietary Inflammatory Index (DII), and medication use. Results: Of 468 participants initially recruited, were included in the final analysis after applying inclusion and exclusion criteria (88.48% female, mean age: 66.16 ± 9.59 years). A significant association was observed between the DII and antihypertensive use (p-value < 0.001), with higher DII scores correlating with increased antihypertensive consumption. Among dietary patterns, the AnMED diet exhibited the strongest association with the DII (p-adjust < 0.001). Predictive modeling revealed a 14.28% increase in antihypertensive use per unit rise in the DII. The AnMED diet was the only pattern significantly linked to improved micronutrient intake, including calcium, magnesium, sodium, and potassium. Conclusions: The DII is a useful tool for assessing the inflammatory potential of diets. Diets with lower DII scores, such as the AnMED diet, may reduce systemic inflammation and improve CV health. Adherence to the AnMED diet may lower blood pressure and reduce reliance on antihypertensive medications, supporting anti-inflammatory dietary patterns for CV disease prevention and management. Full article

The phenomenon of suicide has become a significant global concern, claiming over 800,000 lives annually and resulting in millions of suicide attempts worldwide. In the aftermath of the COVID-19 pandemic, these troubling statistics have worsened, with notable increases in suicidal behavior, especially among vulnerable populations such as the youth, the elderly, and those in socioeconomically disadvantaged groups. This paper aims to explore the impact of the COVID-19 pandemic on suicide rates in Spain, using a theoretical ex post facto analysis. Spain has witnessed an alarming rise in suicide rates, particularly among young people, and a disturbing trend of increased suicidal ideation and self-harm behaviors. While some studies report no significant change in suicide rates during the pandemic, others point to the exacerbating effects of social isolation, economic instability, and public health measures. This study provides an in-depth examination of the psychosocial consequences of the pandemic on mental health in Spain, emphasizing the urgency of the need to address pre-existing inequalities and implement effective suicide prevention measures. Furthermore, it highlights the importance of gender-sensitive strategies and the need for systemic reforms to ensure better mental healthcare access for all segments of society. To achieve this goal, this paper uses a narrative literature review combined with a theoretical ex post facto analysis to assess the influence of the COVID-19 pandemic on suicide patterns in Spain. Full article

Background: The increasing demand for elective surgery makes optimizing preoperative assessment a priority. Value-based healthcare aims to provide the highest value for patients at the lowest possible cost through various mechanisms, including reorganizing care into integrated practice units (IPUs). However, few studies have analyzed the effectiveness of implementing virtually led IPUs for preoperative assessment. Methods: We performed a retrospective observational cohort study including patients undergoing elective surgery at a teaching hospital in Madrid, Spain from 1 January 2018 to 31 December 2023, analyzing changes in surgical complications, efficiency, and patient satisfaction between the pre-implementation (2018–2019) and post-implementation (2020–2023) periods. Anesthesiologists’ satisfaction with the virtual assessments was described. During the post-implementation period, preoperative assessment was reorganized as a virtually led IPU. At the IPU appointment, preoperative testing and physical (including airway) examinations were performed by a nurse anesthesiologist. The results were uploaded to the electronic health records, and asynchronous virtual anesthesiologist assessment using a store-and-forward approach was performed. Digital patient education was carried out over the Patient Portal mobile application. Results: A total of 40,233 surgical procedures were included, of which 31,259 were from the post-intervention period. During the post-intervention period, no increase in surgical complications was observed, while same-day cancellations decreased from 4.3% to 2.8% of the total procedures (p < 0.001). The overall process time did not increase, despite the rising number of surgical procedures per year. Patient satisfaction improved. The median time to complete anesthesiologist assessment was significantly lower for virtual assessment (4.5 versus 10 min (p < 0.001), signifying estimated time savings of 716 person-hours per year. Anesthesiologists agreed that virtual assessment was more efficient than in-person evaluation, and half of the participants agreed that virtual preoperative care improved their work–life balance and reduced burnout. Conclusions: A digitally enhanced value-based model of preoperative care can improve efficiency and satisfaction metrics, reducing unnecessary costs and potentially improving the quality of care. Full article

Introduction: High-performance fiber-reinforced technopolymers for computer-aided design/computer-aided manufacturing (CAD/CAM) of dental restorations offer superior durability and strength. However, exposure to acidic solutions may adversely affect these mechanical properties. Objective: This study aimed to evaluate the flexural properties of a high-strength commercially available CAD/CAM fiber-reinforced dental material in response to water, cola, and artificial gastric acid solutions. Method: Forty bar-shaped specimens (1 × 4 × 13 mm) were fabricated from a pre-polymerized glass fiber-reinforced composite (Trilor disks, Bioloren, Saronno, Italy). Ten specimens were randomly selected for baseline testing. The remaining specimens were subdivided into three groups based on the storage media (n = 10): artificial gastric acid solution, Coca-Cola, and deionized water (37 °C, 48 h). Mean flexural strengths and moduli were statistically compared at a significance level of p < 0.05. Results: No statistically significant change in flexural strength was observed after immersion in the different media. However, there was a statistically significant decrease in the flexural modulus after storage for 48 h, regardless of pH. Conclusion: Fiber-reinforced CAD/CAM technopolymers show promising strength stability in response to varying pH conditions. However, further studies are needed to investigate the material’s long-term strength stability. Full article