Search Results (2,962)

The discovery of dynamical quantum phase transitions (DQPTs) has fundamentally challenged the traditional view that phase transitions only occur in thermal equilibrium. Experimental platforms and 1D numerical methods, like matrix product states (MPS), have made great progress. However, exploring true 2D DQPTs remains difficult due to finite-size limitations and the geometric biases of quasi-1D cylinder mappings. Here, we bypass these limitations by deploying a tree tensor network (TTN) approach. This allows us to directly compute the quench dynamics of the transverse-field Ising model (TFIM) on an open 2D square lattice. Because the TTN architecture naturally mirrors 2D lattice connectivity, we can extract the global Loschmidt echo. Our simulations reveal that while deep quenches yield standard DQPTs, quenching within the ferromagnetic phase produces an anomalous dynamical response. In this regime, the rate function exhibits sharp non-analytic peaks even as the macroscopic order parameter maintains its initial sign. This decoupled behavior strongly indicates that local spin excitations drive 2D DQPTs, rather than the macroscopic domain-wall motions seen in 1D chains. These results provide a quantitative numerical baseline for understanding non-equilibrium quantum matter in higher dimensions. Full article

Chemotherapy-induced mucositis (CIM) is a dose-limiting toxicity of cancer therapy that is mainly associated with mitochondrial dysfunction in epithelial cells. We investigated whether GV1001, a mitochondrial protective peptide from human telomerase reverse transcriptase (hTERT), attenuates 5-fluorouracil (5-FU)-induced mucositis in a murine model. 5-FU induced notable mortality, leukopenia, and mucositis in the gastrointestinal (GI) tract, including tongue, esophagus and small intestine. It promoted epithelial–mesenchymal transition (EMT), nuclear factor kappa-B (NF-κB) activation, systemic and mucosal inflammation, DNA damage, impaired cell proliferation, and apoptosis throughout the GI tract. GV1001 blocked 5-FU–associated mortality, significantly attenuated leukopenia, and notably prevented mucositis. GV1001 also suppressed 5-FU-induced DNA damage, EMT, loss of proliferative capacity, apoptosis, and NF-κB activation in mucosal epithelium. In normal human keratinocytes, 5-FU inhibited the cell proliferation, disrupted mitochondrial function, as evidenced by reduced mitochondrial membrane potential, increased reactive oxygen species (ROS) production, impaired electron transport chain (ETC) complex integrity, decreased ATP synthesis, and cytochrome c release into the cytosol. GV1001 markedly mitigated these 5-FU-induced mitochondrial defects. Taken together, GV1001 mitigates CIM by most likely preserving mitochondrial integrity and function, supporting its potential as a strategy to prevent cancer chemotherapy-associated mucosal injury in patients. Full article

The standard meter water flow prover is widely used due to its mature technology and high work efficiency. However, the process of sending the standard meter for calibration is not only time-consuming and laborious but also introduces deviations due to differences in installation and operating conditions. Therefore, by leveraging the high precision, convenient adjustment, and simple traceability of the piston prover, as well as the good repeatability of the standard meter, this paper proposed a cyclic self-calibration method, which iteratively transmits the calibrated piston value to different flow points of the standard meter through the standard meter itself and the transition flow meter, so as to realize the in situ transmission of the value from the high-precision piston to the wide range of standard meter. The water flow standard device and the cyclic self-calibration method, combining the standard meter and the transfer meter in series and parallel, were introduced; the uncertainty transfer chain was analyzed based on the error transfer theory; and experimental validation was conducted. The results show that the proposed self-calibration method is feasible, that the (0.005~4) m³/h high-precision flow rate of the piston can be extended to the (4~1250) m³/h of standard meters through multiple self-calibration value transfers, and that the expanded uncertainty of the water flow standard device is better than 0.20% (k = 2). Full article

This study proposes a novel dual-role weighting framework for dry port location selection, bridging the gap between commercial logistics efficiency and strategic disaster resilience. Designed to establish a new theoretical evaluation paradigm, the research utilizes the Fuzzy Rough SWARA (FR-SWARA) method and a 12-person expert panel to weigh a comprehensive set of 31 criteria under high-dimensional uncertainty. The findings reveal a decisive hierarchical shift, where spatial and infrastructure-related dimensions significantly outweigh traditional cost considerations. This empirical evidence substantiates the transition from ‘just-in-time’ to ‘Just-in-Case’ logistics architectures. Ultimately, the study reconceptualizes the dry port as a ‘strategic stabilizer’—a critical infrastructure node that absorbs systemic shocks and maintains supply chain continuity during diverse crises, including natural disasters and the COVID-19 pandemic. The proposed weighting framework offers a robust theoretical roadmap for policy and managerial decision-making in volatile geographies. Full article

Although prebiotics and postbiotics support gastrointestinal health, evidence for their combined effects in dogs remains limited. This study evaluated a novel prebiotic and postbiotic supplement in healthy dogs undergoing a dietary transition. Thirty-six healthy adult dogs were randomly assigned to control group (CON, high-protein basal diet with placebo chew) or treatment group (TRT, the same basal diet with chew containing prebiotics [baobab fruit pulp and acacia gum] and postbiotics [inactivated Lactobacillus acidophilus and selected yeast fractions]) for a 28-day formal trial following a 7-day adaptation period. The primary outcomes evaluated included clinical fecal scores, specific biomarkers of intestinal barrier function and inflammation, fecal short-chain fatty acids, and microbiota structure. Following the 7-day adaptation, formal trial baseline, fecal scores were already within the healthy range and remained optimal without differing between groups throughout the study. Compared with CON, the TRT group showed lower fecal calprotectin and serum diamine oxidase levels, and higher fecal butyrate (p < 0.05). Metagenomic analysis revealed increased abundances of Bacteroidota, Oscillospiraceae, Prevotellaceae, and Prevotella in TRT (p < 0.05). Overall, in healthy dogs, this supplementation was associated with favorable microbiota modulation and modulated biomarkers of intestinal barrier and inflammation within normal ranges, without altering clinical fecal endpoints. Full article

The critical role of green consumption in mitigating carbon emissions is widely acknowledged. As a prerequisite for green consumption, consumer green awareness (CGA) plays a pivotal role in advancing sustainable development. This study constructs a comprehensive indicator system for CGA from the three dimensions of “antecedent-behavior-outcome” and measures the CGA levels of 30 provinces in China from 2014 to 2022. Using the Theil index, kernel density estimation, Moran’s I, and Markov chain methods, we analyze its spatiotemporal evolution characteristics. Furthermore, spatial econometric models are applied to explore its driving factors. The results show that China’s CGA exhibits sustained growth during the study period, but regional disparities are widening, driven by inter-regional rather than intra-regional differences. Moreover, China’s CGA gradually demonstrates the long-tailed and multimodal distribution, accompanied by emerging spatial clustering effects. In terms of transition dynamics, CGA demonstrates a short-term “gradient lock”, which is substantially alleviated when spatial spillover effects are incorporated. Additionally, we find that economic development, the advancement of emerging industries, accelerated urbanization, emphasis on education, and policy guidance significantly promote CGA, while overconsumption inhibits CGA. Among these factors, economic development, informatization, e-commerce, education, and policy guidance show significant spillover effects. Full article

The Andean walnut (Juglans neotropica Diels), locally known as tocte, is a keystone tree species of major socio-ecological importance in South American mountain ecosystems, facing severe anthropogenic pressure associated with genetic erosion, habitat fragmentation, and unregulated selective logging. This article presents a case study applying a qualitative phenomenological approach to examine the power relations and institutional failures shaping the sustainable management of its value chain in Imbabura Province, Ecuador. Drawing on 21 in-depth semi-structured interviews with key actors (including woodcarvers, sawyers, traders, and environmental authorities) conducted between March and September 2025 until theoretical saturation was achieved, and supported by thematic analysis in ATLAS.ti, we identified five thematic categories revealing the tension between cultural valuation and market pressure. The findings confirm the existence of a value paradox, whereby high timber demand paradoxically accelerates resource depletion rather than incentivizing conservation, as premature harvesting of young trees undermines the viability of non-timber forest products such as nuts and accelerates the loss of local genetic resources. We conclude that the long-term conservation of the species requires a transition toward polycentric stewardship, community forestry enterprises, and integrated landscape management in which the standing tree is formally recognized as carrying greater ecological and economic value than harvested timber. Full article

South Africa’s food system reflects a growing imbalance between nutritionally rich indigenous foods and the increasing dominance of commercially processed alternatives. Despite well documented health, cultural, and environmental benefits, indigenous leafy vegetables and edible insects remain marginal within formal markets and everyday diets. This systematic review synthesised 141 empirical and theoretical studies to examine how household attitudes, consumption behaviours, and market structures interact to shape the role of indigenous foods in South Africa. The review identifies a consistent pattern in which positive perceptions of indigenous foods do not translate into regular consumption. Rural households continue to utilise these foods as part of seasonal and livelihood strategies, while uptake in urban areas remains uneven and context specific. Emerging interest among certain consumer segments highlights potential for product diversification and market development, particularly where indigenous foods are adapted to align with modern preferences. However, this potential is constrained by weak value chain integration, limited standardisation, and the absence of reliable consumption data. These structural limitations restrict both market participation and consumer access, reinforcing the peripheral position of indigenous foods within the broader food system. The findings suggest improving availability, strengthening markets, and enhancing positioning critical for inclusion of indigenous foods in diets. Full article

The dual circulation strategy represents China’s new-era national development paradigm aimed at achieving sustainable economic growth while promoting global value chain (GVC) upgrading. This study develops an integrated analytical framework that combines a production–demand-based theoretical model with firm-level empirical analysis to examine how domestic and international circulation jointly influence value chain upgrading. Using multiple datasets, including the World Input–Output Database (WIOD), the China Industrial Enterprise Database, and the China Customs Database, the study provides empirical evidence on the mechanisms underlying GVC upgrading. The results show that within the domestic circulation framework, firms positioned further upstream in the value chain experience significantly slower upgrading, reflecting structural constraints such as limited domestic circulation depth and weaker feedback effects. The findings further indicate that dual circulation plays a critical role in overcoming these constraints. Improvements in domestic technological capabilities enhance firms’ competitiveness in global markets, facilitating their transition into higher value-added segments of GVCs. Domestic and international circulation thus operate in a mutually reinforcing manner. Overall, the study highlights the importance of strengthening domestic circulation while deepening international engagement to achieve sustainable and coordinated value chain upgrading. Full article

The deep physiological dormancy of Zanthoxylum armatum DC. seeds severely limits its seedling propagation efficiency. Variable temperature stratification is an effective treatment for promoting dormancy release; however, the metabolic basis underlying this process remains poorly understood. In this study, we utilized a UPLC-MS/MS-based untargeted metabolomics approach, coupled with multivariate statistical analyses (PCA and OPLS-DA), to profile metabolic changes in Z. armatum seeds subjected to variable temperature stratification in a moist sand substrate (15 °C in the dark for 10 days, followed by 4 °C for 20 days). A total of 3687 metabolic features were detected, among which 33 structurally annotated differential metabolites were retained for biological interpretation, including 8 upregulated and 25 downregulated metabolites. Pathway enrichment analysis indicated that α-linolenic acid metabolism and linoleic acid metabolism were markedly altered after stratification. In particular, 9-(S)-HPOTE, colneleate, jasmonic acid (JA), and JA-ACC were significantly reduced, suggesting that attenuation of JA-related oxylipin metabolism may be associated with dormancy release in Z. armatum seeds. In addition, coordinated changes in phenylpropanoid- and cutin/wax-related metabolites implied remodeling of seed-coat-associated metabolism, whereas the accumulation of branched-chain amino acids and the alteration of sulfur- and purine-related metabolites suggested reorganization of metabolic reserves during the transition from dormancy to germination. Overall, these results provide metabolomic evidence that variable temperature stratification is associated with extensive metabolic reprogramming in Z. armatum seeds and highlight JA-related lipid metabolism as a candidate pathway involved in dormancy release. Full article

The emergence of membrane boundaries represents a decisive transition in the origin of life, yet the molecular nature of the earliest abiotic membranes remains uncertain. Existing models based on simple fatty acids, while experimentally tractable, often lack the environmental robustness required under fluctuating prebiotic conditions. Furthermore, the absence of clear pathways linking primitive amphiphiles to later phospholipid systems highlights the need for chemically continuous intermediate frameworks. Here, we explore borate-bridged amphiphile–carbohydrate conjugates as plausible intermediates between simple prebiotic surfactants and modern lipid bilayers. These conjugates arise from low-molecular-weight polyols—including glycerol, butane-1,2,3,4-tetraol, pentane-1,2,3,4,5-pentaol, and hexane-1,2,3,4,5,6-hexitol—reacting with long-chain alkyl ethers and borate species under alkaline conditions, enabling reversible coupling to ribose and other vicinal diol-containing sugars. This chemistry integrates three essential properties for early compartmentalization: hydrolytically robust ether-linked hydrophobic domains, multivalent and highly hydrated headgroups, and environmentally responsive borate coordination. Comparative physicochemical analysis suggests that single-tail alkylglycerol derivatives preferentially form micelles and interfacial films, while di- and tri-tail tetritol and pentitol conjugates favor lamellar assemblies and vesicle formation across realistic prebiotic pH and salinity ranges. Hexitol-based systems, particularly those bearing three hydrophobic chains, may act as membrane-stabilizing components that enhance rigidity and reduce permeability under extreme conditions. We propose that heterogeneous mixtures dominated by two-tail polyol diethers, supplemented by tri-tail stabilizers and surface-active alkylglycerols, could provide mechanically robust, pH-tunable, and sugar-decorated abiotic membranes. Such borate-mediated amphiphiles offer a chemically coherent framework linking carbohydrate stabilization, ether lipid persistence, and dynamic self-assembly, potentially representing a transitional stage in the evolutionary pathway from primitive amphiphilic films to biologically encoded membranes. Full article

The use of renewable energy sources instead of conventional ones, together with the development of efficient electricity storage solutions, represents a central objective of the transition to sustainable and resilient energy systems. In this context, two main development directions are the integration of hydrogen in the energy chain (Power-to-Gas) and the use of batteries, each with specific advantages and disadvantages, compared to internal combustion engines. The purpose of this work was to evaluate the dynamic response time of a hydrogen fuel cell model powered by green hydrogen, under conditions of sudden and instantaneous power demand, for its integration into wind-based renewable energy systems. Experimental research was carried out on an autonomous installation designed to operate continuously for an unlimited duration, simulating the integration of hydrogen produced from wind sources. The novelty consists of the application of an instrumental method for automatic measurement of the response time of a proton exchange membrane hydrogen fuel cell, based on the automatic acquisition and processing of measured electrical signals. The response time of the fuel cell was compared with that of an internal combustion engine based on the classic Carnot cycle, using a dedicated oscilloscope. The load connection time, the current and voltage variation as a function of time were recorded simultaneously. The results show that the response time of the fuel cell is relatively short (approximately 0.3 ms), much lower than that of the internal combustion engine (0.7 s), being of the order of about 2333 times smaller. In conclusion, the hydrogen fuel cell can be effectively integrated into renewable energy systems for the role of an uninterruptible power supply, with an exceptionally fast dynamic response, suitable for applications in regulating and supporting wind-powered networks. Full article

Chondroitin sulfate (CS) chains are major components of the extra- and pericellular matrix in the central nervous system (CNS), and their sulfation patterns influence CNS development and function. Highly sulfated CS preparations, including CS-D- and CS-E-enriched forms, have been shown to facilitate neurite outgrowth in cultured mouse hippocampal neurons. Notably, neurons cultured on CS-D- or CS-E-enriched substrates exhibited the following distinct morphological characteristics: CS-D promoted the extension of multiple short neurites, whereas CS-E induced the formation of a single elongated neurite with a polarization-like morphology. These features are consistent with early stages of neuronal polarization. However, the specific roles of these highly sulfated CS forms in polarization-like morphology remain unclear. In this study, we demonstrate that polarization-like morphological transitions in hippocampal neurons can be modulated on mixed CS-D/CS-E substrates by varying their ratios. Compared with CS-D-enriched substrates, CS-E-enriched substrates more effectively promoted polarization-like neuronal morphology, accompanied by enhanced activation of Src-family kinases. Furthermore, forced activation of Fyn kinase induced morphological changes resembling polarization-like features in a neuroblastoma cell line, even in the absence of CS-D/CS-E mixed substrates. In conclusion, highly sulfated CS subtypes may function as extracellular cues that regulate neuronal morphology via Src-family signaling, with likely involvement of Fyn. Full article

Deposits of insoluble protein plaques, which are mostly composed of fibrils from disease-specific amyloid proteins, are histological markers of various human disorders. These range from non-neuropathic amyloidosis such as light chain amyloidosis or type II diabetes to well-known neuro-degenerative diseases such as Alzheimer’s Disease and Parkinson’s Disease. There are indications that these types of fibrillar suprastructures display biological activity distinct from the individual fibrils they are composed of. Yet, little is known about the mechanisms underlying the assembly of fibrillar suprastructures. An understanding of secondary fibril self-assembly into mesoscopic and macroscopic suprastructures is also critical for their application as novel biomaterial. The paucity of experimental data and theoretical models on fibrillar supra-assembly likely relates to the experimental and conceptual challenges in following this type of assembly on multiple length- and timescales, and in characterizing the distinct morphologies formed. Here, we report that the amyloid dye thioflavin T (ThT) is augmented during self-assembly of isolated lysozyme fibrils. We provide evidence that this augmentation of ThT fluorescence results from the unquenching of fibril-bound ThT during fibril binding. Combining ThT fluorescence, optical density, and fluorescence quenching kinetics with optical and electron microscopy, we propose that fibril self-assembly is driven by a transition from reaction-limited ordered assembly to diffusion-limited random cross-linking of fibrils. Full article

To reduce food waste and mitigate health risks from accidentally consuming spoiled food, freshness-indicating technologies are increasingly demanded. However, conventional colorimetric-based freshness-indicating packaging is limited by instability, subtle color changes, and complex production processes. This study presents a curcumin-based ink suitable for eco-friendly polylactic acid (PLA) food packaging films enabling real-time shrimp freshness monitoring via integrated intelligent packaging. The ink comprised curcumin as the indicator, ethyl cellulose (EC) and polyvinyl butyral (PVB) as binders, and polyethylene glycol 400 (PEG 400) to regulate permeability. Excellent printability was demonstrated by fineness, initial dryness and fluidity tests. It also demonstrated good thixotropic, viscosity, and flow curve properties. Printing minimally affected the PLA films’ mechanical and barrier properties. The indicator label showed high sensitivity, rapid response, and excellent reversibility to ammonia vapor. Practical application in monitoring shrimp spoilage at 25 °C and 4 °C revealed a strong correlation between the distinct color transition of the label and the increase in total volatile basic nitrogen (TVB-N) content and pH value, providing a reliable visual warning before obvious spoilage signs appeared. This work provides a viable integrated indicator packaging strategy for developing intelligent packaging, offering significant potential to reduce food waste and enhance supply chain transparency for perishable goods. Full article