Search Results (16)

Particle number concentration (PN) in vehicle exhaust and ambient air describes the number of ultrafine particles (UFPs) below 500 nm, which are recognized as a toxic and carcinogenic component of pollution and are regulated in several countries. Metal nuclei, ash, and organic matter contribute significantly to the ultrafine particle size fraction and, thus, to the particle number concentration. Exhaust gas filtration is increasingly being used worldwide to significantly reduce this pollution, both on diesel particulate filter (DPF) and gasoline particulate filter (GPF) engines. In recent years, the EU has also funded research projects dealing with the possibilities of retrofitting gasoline vehicles with GPFs. This paper presents the results and compares the PN emissions of different vehicles. An original equipment manufacturer (OEM) diesel car with a DPF is considered as a benchmark. The PN emissions of this car are compared with a CNG car without filtration and with gasoline cars equipped with GPFs. It can be concluded that the currently used GPFs still have some potential to improve their filtration efficiency and that a modern CNG car would still have remarkable possibilities to reduce PN emissions with an improved quality GPF. Full article

Proximate analysis, including ash, volatile matter, moisture, fixed carbon, and calorific value, is a fundamental aspect of fuel testing and serves as the primary method for evaluating coal quality, which is critical for the processing and utilization of coal. The traditional analytical methods involve time-consuming and costly combustion processes, particularly when applied to large volumes of coal that need to be sampled in massive batches. Hyperspectral imaging is promising for the rapid and nondestructive determination of coal quality indices. In this study, a fast and nondestructive coal proximate analysis method with combined spectral-spatial features was developed using a hyperspectral imaging system in the 450–2500 nm range. The processed spectra were evaluated using PLSR, with the most effective MSC spectra selected. To reduce the spectral redundancy and improve the accuracy, the SPA, Boruta, iVISSA, and CARS algorithms were adopted to extract the characteristic wavelengths, and 16 prediction models were constructed and optimized based on the PLSR, RF, BPNN, and LSSVR algorithms within the Optuna framework for each quality indicator. For spatial information, the histogram statistics, gray-level covariance matrix, and Gabor filters were employed to extract the texture features within the characteristic wavelengths. The texture feature-based and combined spectral-texture feature-based prediction models were constructed by applying the spectral modeling strategy, respectively. Compared with the models based on spectral or texture features only, the LSSVR models with combined spectral-texture features achieved the highest prediction accuracy in all quality metrics, with $R_p^2$ values of 0.993, 0.989, 0.979, 0.948, and 0.994 for Ash, VM, MC, FC, and CV, respectively. This study provides a technical reference for hyperspectral imaging technology as a new method for the rapid, nondestructive proximate analysis and quality assessment of coal. Full article

This research assesses the effect of carbonated pyrolysis oil (CPO) derived from scrap car tires on the metallurgical efficiency of coal flotation as a flotation additive. Using a statistical experimental design, the influence of various operational variables, including solid percent of feed pulp and dosages of reagents, i.e., CPO as an additive, diesel oil as a collector, and pine oil as a frother, on the ash content and yield of the final concentrate were investigated. Experimental data vary significantly based on operational conditions, ranging from 6.6% ash content with a 15% yield to 19.1% ash content with a 76.8% yield. The composition of the pyrolysis oil was identified by using Fourier transform infrared spectroscopy (FTIR). The analysis of variance (ANOVA) of experimental results demonstrated that almost all variables had a substantial effect on the flotation responses, positive or negative, depending on the variable or variable interaction. It was discovered that the usage of CPO intensified the total yield and ash content of concentrate in a nonlinear fashion in a range of 15% and 4%, respectively. The results revealed a non-selective interaction effect between CPO and pine oil, as well as competitive adsorption between diesel oil and CPO, which contributed to the curved behavior of flotation measurements. The detrimental effect of CPO on the flotation response of the studied coal sample was also related to the interaction of the hydrophilic groups in the CPO structure and the oxide groups of ash material in coal particles. This work shows the potential of carbonated pyrolysis oil to enhance coal flotation performance and sheds light on the underlying mechanisms. Full article

Multiple Myeloma is a hematological neoplasm that, over the recent few years, has benefited from numerous therapeutic options. Among the latter, CAR-T stands out as the most recent and one of the most promising treatments currently available. Despite its recent introduction, multiple CAR-T products have already been approved, and research regarding cellular therapy is rapidly increasing. We conducted a comprehensive search and review of the available literature, including published studies and abstracts from recent meetings (ASH, ASCO, ASTCT, IMS), regarding Multiple Myeloma and CAR-T therapy. We describe the discovery and research regarding promising targets like the B-Cell Maturation Antigen (BCMA) and others, the origin and nature of CAR-T cells, and the recent introduction of anti-BCMA CAR-Ts Idecabtagene-vicleucel and Ciltacabtagene-autoleucel, which are currently the only approved CAR-T products for MM. Additionally, we discuss non-BCMA-targeting CAR-Ts and their clinical implications. Given the significant impact of cellular therapy, we provide an overview of its limitations and possible adverse implications, as well as related resistance mechanisms. Finally, we describe the current research aimed at improving CAR-T therapy in MM, including structural innovations and new therapeutic approaches, such as in the earlier lines of treatment and maintenance therapy. Full article

Introduction: Urological cancers account for a significant portion of cancer diagnoses and mortality rates worldwide. The traditional treatment options of surgery and chemoradiation can have significant morbidity and become ineffective in refractory disease. The discovery of the CRISPR system has opened up new avenues for cancer research by targeting specific genes or mutations that play a role in cancer development and progression. In this review, we summarise the current state of research on CRISPR in urology and discuss its potential for improving the diagnosis and treatment of urological cancers. Methods: A comprehensive literature search was conducted on databases including PubMed, Embase, and Cochrane Library. The keywords included CRISPR and urology OR prostate OR renal OR bladder OR testicular cancer. Results: CRISPR has been used extensively in a preclinical setting to identify and target genes in prostate cancer, including AR, NANOG, ERβ, TP53, PTEN, and PD-1. Targeting PRRX2 and PTEN has also been shown to overcome enzalutamide and docetaxel resistance in vitro. In bladder cancer, CBP, p300, hTERT, lncRNA SNGH3, SMAD7e, and FOXA1 have been targeted, with HNRNPU knockout demonstrating tumour inhibition, increased apoptosis and enhanced cisplatin sensitivity both in vitro and in vivo. Renal cancer has seen CRISPR target VHL, TWIST1, PTEN, and CD70, with the first in-human clinical trial of Anti-CD70 CAR T cell therapy showing an excellent safety profile and durable oncological results. Lastly, testicular cancer modelling has utilised CRISPR to knockout FLNA, ASH2L, HMGB4, CD24, and VIRMA, with NAE1 found to be over-expressed in cisplatin-resistant germ cell colonies. Conclusions: CRISPR is a cutting-edge technology that has been used extensively in the pre-clinical setting to identify new genetic targets, enhance drug sensitivity, and inhibit cancer progression in animal models. Although CAR T cell therapy has shown promising results in RCC, CRISPR-based therapeutics are far from mainstream, with further studies needed across all urological malignancies. Full article

The presented study deals with the use of legumes intercropped with maize for the production of biogas from silage. The main goal was to find out whether silages made from mixed cultures can be used in biogas production and how the use of such silages affects qualitative and quantitative parameters of the fermentation process compared with the pure maize silage. Variants prepared were pure cultures of maize, bean, lupin, and white sweet clover. In addition, mixed cultures were prepared of maize and individual legumes. Measured values showed that in terms of dry matter (DM) yield, mixed culture silages are almost of the same or even better quality than silage made from the maize monosubstrate. Compared with the maize monoculture silage, the presence of white lupine, white sweet clover, and broad bean in silages statistically significantly increased the content of DM, ash, and acid detergent fiber (by more than 5%). Bean and lupine in mixed silages with maize significantly increased the content of lipids (on average by more than 1.2%). Legumes in silages were significantly decreasing contents of neutral detergent fiber, crude protein, and starch. Production of biogas from silages of maize monosubstrates and mixed substrates of maize with white lupin, maize with white sweet clover, and maize with broad bean was directly proportional to the content of CAR and starch in these substrates. A perspective variant was the mixed substrate of maize and sweet clover from which biogas production was only 6% lower than that from conventional maize silage. The highest yield was recorded in the maize monosubstrate (0.923 m³/kg_VS). Variants of mixed substrates had a yield ranging from 0.804 to 0.840 m³/kg_VS. Full article

The influence of geopolymer binder characteristics on the performance of geopolymer concrete has been extensively investigated. Yet, the influence of aggregate properties has not been thoroughly studied, and it is usually assumed that their effect is the same as in cement concrete. This study investigates the effect of aggregate on the performance of fly-ash-based geopolymer concrete. A systematic experimental study was undertaken to investigate the effect of aggregate parameters, including volume fractions (AVFs), coarse aggregate to the total aggregate ratio (CAR), maximum coarse aggregate size (MAS), and fineness modulus of fine aggregate (FFM) on the compressive strength, slump, apparent volume of permeable pores (AVPPs), and the air content of geopolymer concrete. Response surface methodology (RSM) using the central composite design approach was utilized to design the experiments and analyze the results statistically. The analysis shows that all of the investigated aggregate parameters have significant first-order effects on the measured properties. No significant interaction between any of the investigated parameters was found. The aggregate may alter the geopolymerization processes, whereby SEM-EDS analysis revealed statistically significant variations in the elemental concentrations of the produced paste as the aggregate parameters changed. Quantitative weights were assigned to the effect of the investigated aggregate parameters on the measured properties. Multi-objective optimization was carried out to obtain the best combinations of the investigated parameters. Additionally, the developed contour graphs may provide an effective tool that can be used as a guide in establishing the first trial mixtures. A wide range of consistencies (10–210 mm slump) and compressive strengths (15–55 MPa) can be obtained by controlling the aggregate grading and proportions. Full article

This work presents two types of ultrasonic vibrators in order to comparatively analyze their dust removal properties for microscopic particles. The vibrators were constructed by pasting four piezoceramic (PZT) plates onto the upper surface of a rectangular metal substrate. The longitudinal or bending mode is excited to form a standing wave in the vibrators. The superposition of the longitudinal and bending modes generates a traveling wave. Because the bending and longitudinal vibrations are two different modes, the process of tuning their resonant frequencies to be close is necessary for the traveling wave vibrator. The structural parameters of the vibrators were optimized by the finite element method. During experiments, the dust removal performances of these vibrators were evaluated by dumping cigarette ash or alumina powders randomly on the surface of the prototype vibrators. The measured experimental results indicate that the dust removal performance of the traveling wave oscillator is better than that of the standing wave oscillator. The two operating modes of the traveling wave vibrator produce orthogonal vibration displacements on the vibrator driving points. Vibration in one direction generates friction force, which drives adherent dust particles. Vibration in the other direction provides intermittent contact pressure between the vibrators and deposited dust particles. The synthesized elliptical motion of vibrator particles helps to improve the dust removal characteristics of the vibrators. The simple structure of the developed vibrators makes them the most promising candidates for dust removal from engines, camera lenses, car windows, and navigation systems. Full article

The paper presents the results of research on a composite mixture intended for use in road construction. The purpose of developing the mixture is to be able to use large amounts of industrial waste to produce building material. The waste used are coal slate from the mining industry, shredded rubber waste from used passenger car tires and fly ash from power plants. The mixture (SRFC) consists of unburnt coal-mining slate (S), shredded rubber waste (R), fly ash (F) and cement(C). A test under cyclic loading conditions was carried out on samples prepared from the SRFC mixture, in which the global deformations and local strains caused on the samples were measured. A measurement system using digital image correlation was used for the research. On the basis of the conducted research, it was found that the content of shredded rubber waste significantly influences the deformability of the tested mixtures and allows for the extension of the scope of elastic deformation in which the tested samples work. Full article

Roles of plant-derived cinnamaldehyde, carvacrol, and thymol in the gut and bone health of laying hens was evaluated in the present study. After acclimation for 2 weeks, a total 384 of 52-week-old laying hens were allocated into three groups for 6 weeks: (1) basal diet group (Ctrl), (2) combination of cinnamaldehyde with carvacrol group (CAR+CIN), and (3) blend of cinnamaldehyde with thymol (THY+CIN). The dietary essential oil level was 100 mg/kg. Each treatment group had eight replicate pens (16 birds/pen). The stiffness and ultimate load of the tibiae from both the CAR+CIN and THY+CIN groups were higher than that of the Ctrl group (p < 0.05), along with comparable tibia ash, calcium, and phosphorus content among groups. At the same time, the manipulation of essential oils upregulated the transcription abundances of intestinal barrier proteins to varying degrees, whereas the experimental treatment failed to affect the composition in phyla of cecal microbiota. When compared to the Ctrl group, birds fed the CAR+CIN and THY+CIN diet displayed decreased bone resorption markers, reduced interleukin-1 concentrations, and increased transforming growth factor beta levels in serum. These findings suggest that cinnamaldehyde with carvacrol or thymol in feed of hens could enhance intestinal barrier and improve the mechanical properties of tibiae through structural modelling but not increase the mineral density, which might be involved in suppressing inflammation-mediated bone resorption. Full article

In this work, chemical recycling as an alternative to conventional end-of-life treatments was studied. Two different types of end-of-life tires (ELT), truck tires and mix tires (50:50 mixture of passenger car and truck tires), were pyrolyzed in a batch microwave reactor. The influence of specific power (10, 20, and 30 W/g) on mass distribution was analyzed. The maximum liquid yield was attained at 10 W/g, while the maximum gas yield is obtained at 30 W/g. Liquid fractions were characterized by gas chromatography/quadrupole mass spectrometry (GC/qMS) to identify the main components, and major compounds were quantified. In all samples, limonene (3.76 ± 0.31–6.80 ± 2.37 wt. %) and BTEX (3.83 ± 0.20–1.19 ± 2.80 wt. %) were the main components. Major limonene concentration is obtained in oil produced from truck ELT while higher yields of aromatic compounds are obtained from mix ELT. The maximum BTEX concentration is obtained at 10 W/g being toluene the main compound with a concentration of 2.07 ± 0.42 and 4.63 ± 1.29 for truck and mix ELT, respectively. The separation and purification of these compounds will confer important value to these fractions. Higher yields of the solid fraction are produced when mix tires are pyrolyzed due to the higher concentration of ash in this type of ELT. Recovered carbon black was characterized by measuring the surface area. Full article

The emerald ash borer (EAB), Agrilus planipennis, is an invasive beetle of East Asian origin that has killed millions of ash trees (Fraxinus spp.) in North America and Russia. In September 2020, EAB was detected in Saint Petersburg, a notable event for the metropolitan city. The aim of the present study was to investigate the occurrence and ecology of EAB in Saint Petersburg. The presence of two distinct enclave populations of EAB was revealed, each of which has, most likely, been established through separate events of “hitchhiking” via transport vehicles. Following the invasion, the further spread of EAB in Saint Petersburg was slow and locally restricted, most likely due to climatic factors. This spread by “hitchhiking” suggests that the possibility of the further long-distance geographic spread of EAB in the Baltic Sea region (the EU) is high, both by ground transport (120–130 km distance from EU borders) and ferries that transport cars across the Baltic Sea. In certain cases, the development of EAB on Fraxinus excelsior, based on the stem portion colonized, larval densities, number of galleries, exit holes, viable larvae, and emerged adult beetles, was more successful than in Fraxinus pennsylvanica trees. The observed relatively high sensitivity of F. excelsior to EAB, therefore, casts doubt on the efficacy and benefits of the currently ongoing selection and breeding projects against ash dieback (ADB) disease, which is caused by the fungus Hymenoscyphus fraxineus. Inventory, mapping, and monitoring of surviving F. excelsior trees infested by both ADB and EAB are necessary to acquire genetic resources for work on the strategic long-term restoration of F. excelsior, tackling the probable invasion of EAB to the EU. Full article

MicroRNA and DNA adduct biomarkers may be used to identify the contribution of environmental pollution to some types of cancers. The aim of this study was to use integrated DNA adducts and microRNAs analyses to study retrospectively the contribution of exposures to environmental carcinogens to lung cancer in 64 non-smokers living in Sicily and Catania city near to the Etna volcano. MicroRNAs were extracted from cancer lung biopsies, and from the surrounding lung normal tissue. The expression of 2549 human microRNAs was analyzed by microarray. Benzo(a)Pyrene-DNA adducts levels were analyzed in the patients’ blood by HPLC−fluorescence detection. Correlations between tetrols and environmental exposures were calculated using Pearson coefficients and regression variable plots. Compared with the healthy tissue, 273 microRNAs were downregulated in lung cancer. Tetrols levels were inversely related both with the distance from Etna and years since smoking cessation, but they were not significantly correlated to environmental exposures. The analysis of the microRNA environmental signatures indicates the contribution of environmental factors to the analyzed lung cancers in the following decreasing rank: (a) car traffic, (b) passive smoke, (c) radon, and (d) volcano ashes. These results provide evidence that microRNA analysis can be used to retrospectively investigate the contribution of environmental factors in human lung cancer occurring in non-smokers. Full article

The application of construction waste as an aggregate in subgrade backfilling is an important recycling option. This study analyzed a subgrade backfill material consisting of lime-fly ash construction waste mixture (LFCWM). Compaction and California bearing ratio (CBR) tests were performed on LFCWM under different cement-aggregate ratios (CARs, 3:7, 4:6, 5:5, 8:2). Different normal stresses (100, 200, and 300 kPa) and aggregate sizes (20%, 40%, 60%, 80% of P_4.75) were also evaluated. The experimental results indicated that: (1) when the CAR was 4:6, the optimum water content and the maximum dry density reached their maximum values of 10.1% and 2.03 g/cm³, respectively, the maximum CBR value was 42.5%, and the shear strength reached its maximum value. (2) With an increase in shear displacement, the shear stress showed a rapid initial increase, then a slow decrease, and finally tended to stabilize. (3) Normal stress had a positive effect on the shear strength of the mixture. (4) When P_4.75 was 40%, the shear strength of LFCWM was the maximum. The research results have been successfully applied to road engineering, providing an important reference for the application of construction waste aggregate in roadbed engineering. Full article

The spatial distribution of potentially toxic elements in land near a heavily loaded highway in the West Attica region (Greece) is discussed. This study aimed to investigate the extent of soil contamination with trace elements on land in the margin of highways. The concentration of thirty-five elements in topsoil, car ash, and road sediment bulk samples was determined. Statistical and spatial analysis was applied for evaluating the geochemical dispersion of the examined elements. Geo-accumulation index was estimated. Elements content were compared to criteria and screening values established by the literature. The concentration of As, Cu, Mn, Ni, P, Pb, and Zn in topsoil appeared to be influenced by traffic emissions. It is found that engine exhaust, tyre wear, and brake lining contribute to the As, Ba, Cu, Mn, Ni, P, Pb, and Zn content recorded in topsoil in the margin of highways. The bulk sample of road sediment is moderately contaminated to extremely contaminated with Cu, Mn, Ni, P, Pb, and Zn. Comparison of elements content in topsoil with screening values revealed that Al, As, B, Ba, Cd, Co, Cr, Cu, Fe, Mn, Ni, Sb, V, and Zn pose a potential risk for plants. Full article