Search Results (18)

Periodontal disease in dogs leads to progressive bone loss and adversely impacts overall health. However, cost-effective regenerative strategies are still limited in veterinary practice. This study aimed to develop and evaluate a novel tannic acid (TA)–gelatin-based hydrogel (Gel), incorporating graphene oxide (GO) and hydroxyapatite nanoparticles (HA), as a potential barrier material for guided tissue regeneration (GTR) applications. The hydrogels—Gel, Gel-GO, Gel-HA, and Gel-GO-HA—were characterized for chemical structure, molecular interactions, surface morphology, nanoparticle dispersion, and tensile strength. Cytotoxicity was assessed using L929 fibroblasts (ISO 10993-5), while cell viability/proliferation, morphology, and alkaline phosphatase (ALP) production were evaluated using canine periodontal ligament-derived cells. Results show that crosslinking with tannic acid enhanced the incorporation of graphene oxide and hydroxyapatite nanoparticles via hydrogen bonding into TA–gelatin-based hydrogels. This combination increased surface roughness, reduced degradation rate, and enabled shape memory behavior, critical for guided tissue regeneration (GTR) membranes. The extracts from Gel-HA-GO showed that cytotoxicity was both time- and concentration-dependent in L929 fibroblasts, whereas enhanced cell proliferation and increased ALP production were observed in cultures derived from canine periodontal ligament cells. These findings suggest that TA–gelatin-based hydrogels incorporating GO and HA demonstrated favorable mechanical and physicochemical properties, biocompatibility, and osteogenic potential. These attributes suggest their viability as a promising composite for the development of innovative GTR strategies to address periodontal tissue loss in veterinary medicine. Full article

Purpose: This paper investigates the biomechanical effect of thermoformed aligners equipped with complementary biomechanical attachments (CBAs) on periodontal ligaments (PDLs) during the expansion process of the maxillary arch. The analysis was conducted using advanced simulations based on the finite element method (FEM). Methods: High-resolution 3D CAD models were created for four tooth types: canine, first premolar, second premolar, and first molar. Additional 3D models were developed for aligners, CBAs, and PDLs. These were integrated into a comprehensive FEM model to simulate clinical rehabilitation scenarios. Validation was achieved through comparative analysis with empirical medical data. Results: The FEM simulations revealed the following: for canine, the displacement was 0.134 mm with a maximum stress of 4.822 KPa in the amelocemental junction. For the first premolar, the displacement was 0.132 mm at a maximum stress of 3.273 KPa in the amelocemental junction. The second premolar had a displacement of 0.129 mm and a stress of 1.358 KPa at 1 mm from the amelocemental junction; and first molar had a displacement of 0.124 mm and a maximum stress of 2.440 KPa. Conclusions: The inclusion of CBAs significantly reduced tooth tipping during maxillary arch expansion. Among the models tested, the vestibular CBA demonstrated superior performance, delivering optimal tooth movement when combined with thermoformed aligners. Significance: FEM techniques provide a robust and cost-effective alternative to in vivo experimentation, offering precise and reliable insights into the biomechanical efficacy of CBAs in thermoformed aligners. This approach minimizes experimental variability and accelerates the evaluation of innovative orthodontic configurations. Full article

Objective: This study compared the tooth movement patterns of a power arm and a lever jig during mandibular canine retraction into a premolar extraction space using skeletal anchorage. Methods: A finite element model was developed based on anatomical structures. A mini-implant was placed between the mandibular second premolar and first molar, and canine retraction was simulated using a power arm and a lever jig. The lever jig’s vertical arm lengths were 6 mm, 8 mm, and 10 mm, corresponding to force application distances of 4.5 mm, 6.4 mm, and 8.2 mm from the archwire, matching the power arm. Finite element analysis was performed using linear mechanical properties and an explicit method. Results: With the power arm, increasing vertical length led to greater extrusion, while the posterior force remained unchanged. The lever jig also showed increased extrusion with length but to a lesser extent. Posterior force increased proportionally with the lever jig length. Initial displacement analysis showed greater extrusion and distal tipping with the power arm, while the lever jig suppressed extrusion and facilitated controlled tipping. Stress analysis revealed a more uniform periodontal ligament stress distribution with the lever jig. Conclusion: The lever jig minimizes extrusion and enhances force concentration posteriorly, promoting efficient distal movement. Full article

The periodontal ligament (PDL) is a fibrous connective tissue that anchors the tooth-root surface to the alveolar bone with specific orientations. It plays a crucial role in functional restoration, optimal position stabilities, biomechanical stress transmission, and appropriate tissue remodeling in response to masticatory loading conditions. This pilot study explored spatial microarchitectures to promote PDL orientations while limiting mineralized tissue formation. A computer-designed perio-complex scaffold was developed with two parts: (1) PDL-guiding architectures with defined surface topography and (2) a bone region with open structures. After SEM analysis of micropatterned topographies on PDL-guiding architectures, perio-complex scaffolds were transplanted into two-wall periodontal defects in the canine mandible. Despite the limited bone formation at the 4-week timepoint, bone parameters in micro-CT quantifications showed statistically significant differences between the no-scaffold and perio-complex scaffold transplantation groups. Histological analyses demonstrated that the PDL-guiding architecture regulated fiber orientations and facilitated the functional restoration of PDL bundles in immunohistochemistry with periostin and decorin (DCN). The perio-complex scaffold exhibited predictable and controlled fibrous tissue alignment with specific angulations, ensuring spatial compartmentalization for PDL tissues and bone regenerations. These findings highlighted that the perio-complex scaffold could serve as an advanced therapeutic approach to contribute periodontal tissue regeneration and functional restoration in tooth-supporting structures. Full article

The quad-helix and W-arch are commonly used appliances for expanding the dental arch in orthodontic treatment. However, differences in performance between these two expanders remain unclear, and no guidelines exist for selecting one over the other. The purpose of this study was to investigate whether there were differences in dental arch expanding ability between these appliances. Maxillary arch expansions were simulated using the finite element method. The expander was assumed to be an elastic beam, while the teeth and alveolar bone were treated as rigid bodies. The periodontal ligament (PDL) was modeled as a nonlinear elastic material. The teeth moved in the same direction as the initial movement caused by the elastic deformation of the PDL. The right and left canines, premolars, and first molars were expanded symmetrically in either parallel or fan shapes. When the wire diameter of the W-arch was set to 0.032 inches its stiffness became equivalent to that of a quad-helix with a wire diameter of 0.036 inches. Canines and premolars were expanded through tipping movements. The molars initially tipped buccally, then became upright and moved bodily. Both expanders expanded the arch in almost the same manner. There was no difference in arch expansion ability between the W-arch made of 0.032-inch wire and the quad-helix made of 0.036-inch wire. The W-arch may be preferred as the first choice due to its simpler structure compared to the quad-helix. Full article

Background: In order to reduce the prolonged duration of orthodontic treatment, several surgical techniques have been proposed over the years. Corticotomy and piezocision are the two most widely used techniques, and, given the lack of consensus in the literature, along with the renewed interest in these approaches in recent years, the primary objective of this study is to evaluate their effectiveness in accelerating canine retraction in patients requiring extraction of the upper first premolar and, as a secondary objective, to assess if there is a worsening of periodontal health and how the surgical approach is perceived by the patient. Methods: An electronic search was performed on PubMed, Scopus, Web of Science, Embase, and the Cochrane Central Register of Controlled Trials (CENTRAL) up to 30 November 2024. The PRISMA statement was adopted for the realization of the review, and the Cochrane Collaboration’s risk of bias assessment tool (RoB 2) was used to assess the studies’ quality. Results: After full text assessment, fifteen randomized clinical trials (14 split mouth design, 1 single-blind, single-center design) covering 326 patients (mean age 20, 19 years) were included. The data collected reveal that corticotomy accelerates canine retraction by 1.5 to 4 times, while piezocision achieves retraction 1.5 to 2 times faster, making corticotomy the most effective technique. No statistically significant adverse effects on periodontal ligament, molar anchorage loss, or root resorption were observed following the two surgical techniques. In addition, patients reported experiencing mild to moderate pain. Conclusions: Corticotomy and piezocision are effective techniques for accelerating upper canine retraction in extraction cases, significantly reducing the overall duration of orthodontic treatment. Full article

Background: The mechanical properties of either alveolar bone or periodontal ligaments under orthodontic loading, as well as orthodontic tooth movement, have been studied in recent years using computational approaches. In previous studies, we developed a theoretical mathematical approach that uses a weighting coefficient of the summed resistance of periodontal structures, namely the bone and periodontal ligaments, in relation to apex movement, the center of rotation, orthodontic force loading, and time in order to quantify the biological response to orthodontic biomechanics. Methods: We analyzed the distal retraction of three maxillary canines and integrated the clinical data obtained in the previously developed mathematical programs. Results: The values of the $\left(\sigma \right)$ weighting coefficient of the tissue resistance were interpreted in the context of the clinical data obtained: the smaller the value of ($\sigma )$, the higher the actual tissue resistance, with a greater difference between the crown and root movement; also, the higher the value of ($\sigma )$, the lower the actual tissue resistance, with a small difference between the crown and apex movement. Conclusions: The clinical interpretation of the results allows us to set a premise for the refinement of the mathematical programs so that we can use them in assessing the orthodontic biomechanics of larger patient groups over longer periods of time and create premises of treatment protocol simplification and adjustment. Full article

A novel water-soluble root canal filling material based on sodium iodide (NaI) has been developed to overcome the limitations of existing iodine-based formulations. However, the biological stability of this approach in animal studies remains unverified. This study evaluated the biocompatibility of NaI compared to commercial root canal filling materials (Calcipex II and Vitapex^®) in pulpectomized canine teeth to assess its clinical applicability. Following a four-week observation period, none of the experimental groups exhibited tooth mobility or fistula formation. Radiographic and micro-CT analyses revealed no radiolucency in periapical lesions. Histopathologic evaluation demonstrated the absence of inflammatory responses in periapical regions across all material groups, with histological inflammation scoring 0. High-magnification histological examination of periapical areas showed well-preserved periodontal ligament tissue in all groups. Despite certain limitations of NaI-based fillings in the pulp cavity, including loss of radiopacity and tooth discoloration, NaI demonstrates potential as a safe and effective alternative for pulp filling material, particularly due to its minimal risk of root resorption and inflammatory response. Full article

Periodontal disease affects about 80% of dogs, highlighting the importance of addressing periodontitis in veterinary dental care. The periodontal ligament (PDL) is a key structure holding the potential to regenerate the entire periodontal complex. This work presents an in vitro model of canine PDL-derived cell cultures that mimic the PDL’s regenerative capacity for both mineralised and soft tissues. Explant outgrowth-derived PDL cells were cultured under standard conditions in osteoinductive medium and with hydroxyapatite nanoparticles (Hap NPs). Cell behaviour was assessed for viability/proliferation, morphology, growth patterns, and the expression of osteogenic and periodontal markers. Osteogenic conditions, either achieved with osteoinducers or an osteoconductive biomaterial, strongly promoted PDL-derived cells’ commitment towards the osteogenic phenotype and significantly increased the expression of periodontal markers. These findings suggest that cultured PDL cells replicate the biological function of the PDL, supporting the regeneration of both soft and hard periodontal tissues under normal and demanding healing conditions. This in vitro model will offer a platform for testing new regenerative treatments and materials, ultimately contributing to canine dental care and better outcomes. Full article

To clarify the mechanics of tooth movement produced by a unique distalizer, Class II Carriere Motion appliance (CMA), in which the maxillary canine is connected to the maxillary first molar with a stiff bar, long-term tooth movement was simulated by the finite element method (FEM). The FEM models of the maxillary canine, premolars, and first molar were made based on a dental study model. The periodontal ligament (PDL) was constructed on the root and assumed to be a nonlinear elastic material. The teeth and the alveolar bone were assumed to be rigid bodies. The tooth moved by accumulating the initial movement produced by the elastic deformation of the PDL. When retraction force was applied to the canine from the mandibular dentition, the canine tipped or rotated clockwise and extruded due to the vertical component of the retraction force. The molar and premolars also tipped and moved distally, but hardly extruded because the vertical force did not act on them. As a result of these tooth movements, the canine protruded from the dentition. An interproximal space was created between the canine and the lateral incisor. These movement patterns were similar to those in other clinical studies using the CMA. Full article

The purpose of this study was to investigate the functional load capacity of the periodontal ligament (PDL) in a full arch maxilla and mandible model using a numerical simulation. The goal was to determine the functional load pattern in multi- and single-rooted teeth with full and reduced periodontal support. CBCT data were used to create 3D models of a maxilla and mandible. The DICOM dataset was used to create a CAD model. For a precise description of the surfaces of each structure (enamel, dentin, cementum, pulp, PDL, gingiva, bone), each tooth was segmented separately, and the biomechanical characteristics were considered. Finite Element Analysis (FEA) software computed the biomechanical behavior of the stepwise increased force of 700 N in the cranial and 350 N in the ventral direction of the muscle approach of the masseter muscle. The periodontal attachment (cementum–PDL–bone contact) was subsequently reduced in 1 mm increments, and the simulation was repeated. Quantitative (pressure, tension, and deformation) and qualitative (color-coded images) data were recorded and descriptively analyzed. The teeth with the highest load capacities were the upper and lower molars (0.4–0.6 MPa), followed by the premolars (0.4–0.5 MPa) and canines (0.3–0.4 MPa) when vertically loaded. Qualitative data showed that the areas with the highest stress in the PDL were single-rooted teeth in the cervical and apical area and molars in the cervical and apical area in addition to the furcation roof. In both single- and multi-rooted teeth, the gradual reduction in bone levels caused an increase in the load on the remaining PDL. Cervical and apical areas, as well as the furcation roof, are the zones with the highest functional stress. The greater the bone loss, the higher the mechanical load on the residual periodontal supporting structures. Full article

Autotransplantation is a potential treatment alternative when orthodontic traction of an impacted tooth is difficult. In this article, we describe two cases of guided autotransplantation of an impacted canine using a computer-aided designed and manufactured surgical template. The impacted canine was segmented on preoperative cone-beam computed tomography images to ensure a sufficient periodontal ligament space and placement of the donor tooth with the least pressure on it. The canine was virtually transposed using a simulation program considering the adjacent teeth. The surgical template, which was connected to the occlusal stop on adjacent teeth, was designed and 3D-printed with polymer resin. The recipient site was prepared using the surgical template, followed by immediate transplantation of the surgically extracted canine into the socket. The transplanted donor tooth was positioned in planned infra-occlusion to prevent occlusal interference. It was then splinted with the adjacent teeth for initial stabilization. During follow-up, one transplanted tooth showed pulp canal obliteration and the other had suspected pulp necrosis; endodontic treatment was performed. One year after the procedure, the periradicular condition of both teeth was favorable. Full article

Objectives: The current study aimed to perform an in vivo examination using a critical-size periodontal canine model to investigate the capability of a 3D-printed soft membrane for guided tissue regeneration (GTR). This membrane is made of a specific composition of gelatin, elastin, and sodium hyaluronate that was fine-tuned and fully characterized in vitro in our previous study. The value of this composition is its potential to be employed as a suitable replacement for collagen, which is the main component of conventional GTR membranes, to overcome the cost issue with collagen. Methods: Critical-size dehiscence defects were surgically created on the buccal surface of the roots of canine bilateral mandibular teeth. GTR treatment was performed with the 3D-printed membrane and two commercially available collagen membranes (Botiss Jason^® and Smartbrane-Regedent membranes) and a group without any membrane placement was considered as the control group. The defects were submerged with tension-free closure of the gingival flaps. Histologic and histometric analyses were employed to assess the periodontal healing over an 8-week experimental period. Results: Histometric evaluations confirmed higher levels of new bone formation in the 3D-printed membrane group. Moreover, in all defects treated with the membranes, the formation of periodontal tissues, bone, periodontal ligaments, and cementum was observed after 8 weeks, while in the control group, only connective tissue was found in the defect sites. There was no clinical sign of inflammation or recession of gingiva in any of the groups. Significance: The 3D-printed gelatin/elastin/sodium hyaluronate membrane can be safe and effective for use in GTR for periodontal tissue regeneration therapies, with better or comparable results to the commercial collagen membranes. Full article

The maxillary sinus is a common anatomic limitation for orthodontic tooth movement. The effect of orthodontic forces on a particular anatomy can be studied using finite element analysis (FEA). Our study aimed to determine the effect of different tooth penetration depths into the maxillary sinus floor (MSF) on the orthodontic force system for bodily tooth movement. Using the cone-beam computed tomography of a patient with low MSF, we modeled the geometry of canine, premolar, and molar teeth with their periodontal ligaments and the alveolar bone surrounding them. The models were manually modified to simulate different root penetration depths. Thereafter, the center of resistance and stress distributions for teeth penetrating into the MS were determined using FEA. Moreover, the force systems for teeth with a low MSF to varying degree were evaluated based on the FEA results. During orthodontic tooth movement, the individual differences in the periodontal anatomy should be considered. The CR position decreases with the penetration depth, while the average hydrostatic stress in the PDL increases rapidly. In this paper, we present the correction coefficients of the orthodontic force and moment for a tooth penetrating into the MSF, which is necessary for personalized treatment planning. Full article

The aim of this study was to evaluate the tooth movement efficacy of retraction springs made of a new β-titanium alloy, “gum metal”, which has a low Young’s modulus and nonlinear super elasticity. Using double loop springs incorporated into an archwire made of gum metal (GUM) and titanium molybdenum alloy (TMA), the maxillary anterior teeth were moved distally to close an extraction space. The long-term movements were simulated by the finite element method. Its procedure was constructed of two steps, with the first step being the calculation of the initial tooth movement produced by elastic deformation of the periodontal ligament, and in the second step, the alveolar socket was moved by the initial tooth movement. By repeating these steps, the tooth moved by accumulating the initial tooth movement. The number of repeating calculations was equivalent to an elapsed time. In the GUM and TMA springs, the anterior teeth firstly tipped lingually, and then became upright. As a result of these movements, the canine could move bodily. The amount of space closure in GUM spring was 1.5 times that in TMA spring. The initial tipping angle of the canine in the GUM spring was larger than that in the TMA spring. The number of repeating calculations required for the bodily movement in the GUM spring was about two times that in the TMA spring. It was predicted that the speed of space closure in the GUM spring was smaller than that in the TMA spring. Full article