Search Results (136)

Under the background of global urbanization, the impacts of urban expansion on biodiversity are becoming increasingly significant. Butterflies, as key ecological indicator species and important pollinators, are crucial for studying population dynamics and their driving factors in order to support biodiversity conservation and environmental governance. Butterflies also have important ecological value in urban biodiversity conservation and landscape maintenance. Currently, in many countries, especially developing countries, impervious surfaces and vegetation cover are undergoing significant changes as urbanization intensifies. Existing studies have begun to focus on the effects of these changes on biodiversity conservation. However, most studies primarily focus on the single effects of these two factors on biodiversity, while systematic assessments of their potential interactive effects remain limited. In this study, monthly butterfly population monitoring was conducted in Kunming, China, for two consecutive years. The study aimed to clarify the composition of butterfly diversity in Kunming and to explore the effects of impervious surfaces and vegetation cover on butterfly species richness, abundance, Shannon index, Simpson index, and community structure. The results showed that impervious surface was the primary driving factor influencing both butterfly richness and abundance, while the influence of vegetation cover was relatively limited. The Shannon and Simpson indices were not significantly influenced by either environmental factor. Environmental filtering caused by impervious surfaces and vegetation cover drove species turnover processes. Consequently, butterfly communities exhibited the highest heterogeneity in areas characterized by low impervious surfaces and high vegetation cover. In contrast, areas with high impervious surfaces and low vegetation cover tended to show a pattern of community homogenization. Urban planning should balance the control of impervious surface proportions with the appropriate enhancement of vegetation cover and plant diversity. Such measures may mitigate the negative impacts of intensified urbanization on butterfly diversity to some extent. Full article

Monarch butterflies have declined in both eastern and western populations. Conservation initiatives that support this imperiled species are being implemented in lands managed by the energy and transportation sectors. Vegetation management strategies that encourage the presence of milkweed (Asclepias spp.), the larval host of monarch butterflies (Danaus plexippus), or floral resources to support pollinators are being practiced across North America; however, survey methods to evaluate the success of these strategies vary in accuracy and scalability. In this study, we compared five methods to quantify milkweed stem density and land cover estimates: (1) Site al, (2) Transect plot, (3) Square plot, (4) Large transect (informed by the Monarch CCAA methodology), and (5) Machine learning of images collected by UAVs. These methods encompass full coverage ground counts, partial ground counts, and aerial imagery using object-based image analysis. Sites included distribution, transmission, and gas line ROWs, solar arrays, and transportation easements. We found that Site al and Machine learning most consistently quantified milkweed stem density across sites. Partial ground count methods were likely to over or underestimate milkweed populations. Habitat characteristics (woody, broadleaf, grass, and bare ground) estimates were inconsistent across method and site. The intent of this study was to provide land managers with insight as to the most accurate, efficient, and economical approach to quantify milkweed populations and habitat characteristics. Full article

Monarch butterfly numbers in North America have been declining since the early 2000s. This review focuses on the causes of this decline in the eastern migratory population, found roughly east of the Rocky Mountains, and ways to achieve population sustainability. Drivers of eastern North American monarchs’ decline include (1) the loss of breeding habitat, caused mainly by the loss of their milkweed host plants in agricultural fields after adoption of genetically modified, herbicide tolerant corn and soybeans, (2) weather, particularly warmer and drier conditions that could push monarchs farther north and lead to lower numbers, and (3) increasing insecticide use. Making habitat broadly available to monarchs across their migratory cycle will make them more resilient, and better able to survive weather-driven declines, and even small tracts of land can provide needed habitat. Sites that are isolated from other habitat patches contain higher egg, larva, and adult monarch densities. Good monarch habitat should include a diversity of native milkweed species and nectar plants that will thrive in local growing conditions. Monarch egg and larva density increases with increasing milkweed density and monarch survival is higher when larvae are less crowded, so a good general rule is that more milkweed is better. Full article

Background: Skipper butterflies (Hesperiidae) are a morphologically distinctive lineage within Papilionoidea, yet relationships among many groups remain difficult to resolve, and mitochondrial genomic resources remain limited for some tribes, including Celaenorrhinini. Methods: We sequenced and characterized the complete mitochondrial genome of Celaenorrhinus victor using Illumina short-read sequencing. Gene content and organization were annotated, codon-usage patterns were assessed across Celaenorrhinus using relative synonymous codon usage and multiple compositional/selection tests (ENC–GC3s, neutrality, and PR2 analyses), selective constraints were evaluated using Ka/Ks for 13 protein-coding genes, and phylogenetic relationships were inferred with a partitioned maximum-likelihood analysis of 66 complete hesperiid mitogenomes. Results: The circular mitogenome of C. victor is 15,180 bp and contains the typical 37 genes (13 protein-coding genes, 22 tRNAs, and two rRNAs) plus an A + T-rich control region, with an overall A + T content of 79.64%. Gene order and orientation match those of other Celaenorrhinus and hesperiid mitogenomes. All protein-coding genes use standard invertebrate mitochondrial start codons (with cox1 initiating with TTG) and terminate with complete TAA stop codons. Codon usage is strongly biased toward A/U-ending codons and is broadly similar among five sampled Celaenorrhinus mitogenomes; ENC–GC3s, neutrality, and PR2 analyses indicate a predominant influence of A + T-directed mutational pressure with additional effects beyond base composition. Ka/Ks values for all 13 protein-coding genes were <1, consistent with pervasive purifying selection; cox genes were the most conserved, whereas several NADH dehydrogenase subunit genes evolved comparatively faster. The phylogeny recovered monophyletic Celaenorrhinini and a well-supported Celaenorrhinus clade, placing C. victor as sister to Celaenorrhinus consanguineus, while deeper nodes among major hesperiid lineages showed only moderate support in parts of the tree. Conclusions: This study provides a new mitogenomic resource for Celaenorrhinini and a comparative reference for codon usage and selective constraints within Celaenorrhinus, supporting the placement of C. victor within Hesperiidae while highlighting remaining uncertainty at deeper hesperiid divergences. Full article

It is widely accepted that preserving biodiversity requires transformative change and, perhaps foremost, demands a paradigm shift in economic scholarship. The prevailing neoclassical growth-based status quo is too often detached from nature and, as a result, ill-equipped to offer insights on how to halt biodiversity loss. This perspective paper builds on the famous cowboy–spaceman economy distinction by presenting an analogy from the natural world. Proposals from post-growth schools of economic thought on how to induce metamorphosis of the growth-based caterpillar economy are described. Thereafter, an exploration is undertaken of a butterfly economy based on three propositions: embed economics in ecosystems, in systems thinking, and in human–nature relationships. Herein, examples are provided of nature-literate economics, policy implications are discussed, and an actionable research agenda is outlined. The perspective closes by drawing on discussions from conservation sciences to inspire the design of an economic framework conducive to healthy human–nature relationships. Full article

The Poweshiek skipperling Oarisma poweshiek (Parker, 1870) (Lepidoptera: Hesperiidae) is an endangered prairie obligate butterfly native to the north central United States and southern Canada. Conservation efforts for this species rely on ex situ approaches for population augmentation and reintroductions. As such, improving our understanding of the behaviors of Poweshiek skipperlings and maximizing their reproductive output in an ex situ setting are critical for the success of associated conservation initiatives. In this study, we examined the frequency of breeding behaviors exhibited by Poweshiek skipperlings in relation to various environmental factors: sunlight intensity (measured in lux), ambient temperature, and age. Sunlight intensity was a significant predictor of breeding behavior frequency, but we did not detect an effect of ambient temperature on breeding behavior. We also documented a generally negative relationship between age and breeding behavior frequency for both sexes. The results of our study underscore the importance of natural sunlight in encouraging breeding behaviors in an ex situ conservation environment. Ex situ observations also can help identify environmental conditions that promote high levels of Poweshiek skipperling activity, which could be used to optimize the timing of in situ population surveys. Full article

The decline of the monarch butterfly population in western North America has been documented by 28 years of community scientist counts from overwintering sites in California. However, no long-term studies on the status and dynamics of monarch populations during spring to fall are available. Using multiple datasets from online recording sites, social media, and personal observations, our study over 13 years shows increases and declines in warm-season populations in the Pacific Northwest (PNW) on a 2 to 4-year cycle that corresponds with fluctuations in overwintering populations. Overall, there was little evidence of a systemic decline in the abundance of monarchs in the PNW. Monarchs were reported in all PNW states and territories in all years, with the earliest annual first appearance in Oregon and the latest in British Columbia and a maximum seasonal range of 200 km north of the US/Canada border. Relatively equal numbers of unique sightings originated from each of three community scientist online recording platforms, with a larger number of unique sightings from personal observations/communications and multiple social media sites. The value of community scientist sightings for the rapid detection of substantial changes in populations was highlighted in August 2024 when weekly sighting data showed a substantial decline in PNW populations that may have been caused by poor survival of immature stages during a July heatwave. The conservation of monarchs in western North America will benefit from contemporary and high-value data on phenology and population dynamics, which we show can be provided by community science observations. Full article

Morpho butterflies have the potential to act as a bioindicator species, since they are sensitive to changes in land uses and forest degradation; there have been few population studies of butterflies in the Caribbean region of Colombia. In this study, we aimed to analyze and characterize the population structure of M. helenor peleides and evaluate its variation across different land uses. We used the capture-mark and release technique, with 50 VanSomeren–Rydon type traps distributed in five sampling units with different land uses (forest, restoration areas, and pasture areas); temperature and luminosity were also measured. Butterflies were wing-marked and subsequently released. Sampling was carried out between June and September 2023. Jolly–Seber population models were constructed using the R code to obtain population size (N_i), survival rate, and recruitment; also, other parameters were analyzed (abundance of the imagines, average sex ratio-ASR, displacement, permanence, and age). A total of 876 butterflies were tagged and released, and 33.7% were recaptured. Butterfly abundance was concentrated in conserved sampling units in the forest. The N_i ranged from 25 to 845 individuals within the population, and individual displacement and permanence were restricted in the forest, ASR was significantly male-skewed. The land use directly influenced the population structure of M. helenor peleides, suggesting that conserved areas are key to population persistence. Full article

We studied Lycaena helle (Lepidoptera: Lycaenidae) population genetics in lowlands and mountains of East-Central Europe using the microsatellite markers previously applied in population studies mainly in mountains of Western Europe. As in the West, the East-Central populations are genetically diverse (mean expected/observed heterozygosity 0.67/0.49), affected by drift processes (mean inbreeding coefficient 0.277) and widely differentiated (mean F_ST 0.209). The Polish lowland populations, all of them bivoltine in contrast to mountain populations, are less differentiated than Romanian populations, which are bivoltine in low and univoltine in high altitudes. The lowland Romanian population Vad is extremely genetically impoverished. A transferred CZ population from Western Europe is impoverished relative to its donor population, but the genetic parameters remain within a range of other studied so far. Dendrogram of allelic frequencies suggests that the populations form two branches, one rooted in southeastern Poland and branching towards Carpathians in Romania, one encompassing populations in central and northern Poland. We conclude that the lowland Romanian populations, plus populations in unglaciated southeastern Poland, represent sites where the species survived the glacial cycles in situ, comprising rear edge of subsequent upslope expansion, while northern Poland was colonised from more easterly regions. Full article

Loeselia mexicana (Polemoniaceae) is a Mexican shrub with significant medicinal value since pre-Hispanic times. Despite its ethnobotanical importance and apparent role in supporting pollinator communities, detailed information about its reproductive biology remains limited, hindering conservation efforts for this increasingly harvested species. We investigated the reproductive ecology of L. mexicana across two flowering seasons (2023–2024 and 2024–2025) in central Mexico through an integrated approach examining flowering phenology, floral morphology, sexual maturation sequence, nectar characteristics, floral visitors, and breeding system experiments. Flowering occurs from September to March, peaking in October. Flowers exhibit protandry, with anther dehiscence on days 1–2 and stigma receptivity from day 2 onward (flower lifespan: 2.85 ± 0.11 days). Maximum nectar production (1.46 ± 0.05 µL per flower; 193.13 ± 8.8 mg/mL) coincided with peak visitor activity. Despite possessing classic ornithophilous traits, we recorded 21 floral visitor species (5 hummingbirds, 3 hymenopterans, 13 butterflies) with similar visitation patterns, challenging previous assumptions about pollination specialization. Controlled pollination experiments confirmed self-incompatibility, with cross-pollination producing significantly more seeds than autonomous selfing. Our findings reveal that L. mexicana maintains a generalized pollination system, while protandry and self-incompatibility enforce outcrossing, providing critical baseline information for conservation strategies. Full article

Host plant limitation poses a major threat to the endangered specialist butterfly Luehdorfia chinensis Leech, 1893, whose larvae are oligophagous at the species level on Asarum spp., while local populations often appear monophagous, depending on the host plants (A. sieboldii Miq. or A. forbesii Maxim.) available in their habitat. To simulate natural starvation caused by host plant scarcity, third- to fifth-instar larvae were subjected to a three-day deprivation treatment, and the effects on individual fitness traits—including larval development, pupal duration, and adult fecundity—were assessed, along with population dynamics. Starvation significantly prolonged larval development, shortened the pupal stage, reduced female fecundity, and markedly decreased key population parameters, such as the intrinsic rate of increase (r_m) and the net reproductive rate (R₀). Population projections further indicated that repeated starvation stress could reduce population size by more than 83% within two years, potentially intensifying genetic drift, inbreeding depression, and demographic instability, ultimately increasing the risk of extinction. These findings provide direct evidence that host plant limitation drives population decline in L. chinensis, contribute to the broader understanding of global butterfly declines, and underscore the critical importance of conserving and restoring essential habitat resources. Moreover, they highlight the relevance of the resource-based habitat concept for the effective protection of specialist species. Full article

Mount Falakro in Northern Greece historically hosted populations of the Balkan-endemic butterfly Polyommatus orphicus and its larval host plant Onobrychis alba. In this study, we surveyed six historically confirmed localities during the peak flight period of P. orphicus in 2024, but neither the butterfly nor the host plant were detected. While the historical data on both species are scarce and often imprecise, our field observations indicate severe habitat degradation, dominated by overgrazing and suspected climate-driven shifts. Habitat conditions were assessed qualitatively, with special attention to limestone substrates previously known to support O. alba. Although definitive absence cannot be statistically confirmed, the lack of detection in previously occupied sites raises urgent concerns about possible local extinction. Our findings suggest that both species may already be extirpated from parts of their former range. This case study underscores the conservation relevance of absence data and highlights the importance of site-based monitoring in mountainous ecosystems undergoing rapid environmental change. Long-term surveys, regulated grazing, and post-disturbance habitat restoration are urgently needed to clarify the conservation status of these species and guide future management strategies. Full article

Habitat and climate changes driven by human activities are altering the distribution of organisms globally. In South Korea, recent temperature increases have exceeded twice the global average, and habitats have markedly changed and shrunk due to urban development driven by population growth and economic expansion. Despite its high biodiversity and over 500 years of preservation, Gwangneung Forest in South Korea has experienced habitat alterations due to the urbanization of surrounding rural areas since the 1990s. In this study, we aimed to evaluate how butterfly communities respond to urbanization and climate change using long-term monitoring data (1998–2015) from the conserved Gwangneung Forest. We considered the thermal adaptation types (cold-, warm-, and moderately adapted species), habitat types (forest edge, forest inside, and grassland), diet breadth (monophagous, oligophagous, and polyphagous), and wingspan of butterflies. Linear regression analysis of the abundance trends for each species revealed that cold-adapted species experienced population declines, while warm-adapted species showed increases. Changes in butterfly abundance were associated with both thermal adaptation type and wingspan, with larger, more mobile species showing greater resistance to habitat loss in surrounding areas. To preserve butterfly diversity in Gwangneung Forest and across South Korea, it is crucial to conserve open green habitats—such as gardens, small arable lands, and grasslands—within urban areas, especially considering the impacts of climate change and habitat loss, which disproportionately affect smaller species with limited mobility. Full article

The Brazilian Atlantic Forest is currently reduced to a few, small fragments surrounded by anthropic landscapes. Urban forest remnants play an important role in housing biodiversity in urbanized areas and enabling species dispersion between larger natural areas. Describing and monitoring the biodiversity in these anthropized environments is a useful tool for urban ecology and species conservation. By compiling long-term data, this study proposes a species list of tropical diurnal butterflies for an Atlantic Forest remnant in Southeastern Brazil and describes the temporal diversity patterns of the community. Inventories were carried out from the 1970s to 2000 to record butterfly incidence data in a 250 ha fragment of seasonal, semideciduous Atlantic Forest, resulting in a species list of 706 species presented here for the first time for the studied area. From 1998 to 2000, weekly standardized observations enabled inferences on temporal trends in butterfly diversity. Total species richness peaked in the climatic transitions, and a cycle of 52 weeks was reported for beta diversity. Butterfly families lack synchronized temporal fluctuations. Nymphalidae and specifically the fruit-feeding nymphalids were considered good surrogates for short-term studies and monitoring community changes. Full article

Protected areas play a key role in containing and protecting most of the endemic biodiversity of megadiverse places, underscoring their importance as custodians of biological richness. Colombia, one of the most species-rich countries, also has one of the highest rates of deforestation globally. The Colombian National Natural Parks (NNPs) system is one of the mechanisms for protecting natural landscape ecosystems and biota. Based on the role of butterflies as bioindicators in biodiversity mapping, we compiled records of endemic butterfly species from entomological collections and the literature to assess the importance of protected areas in endemic species conservation. The NNPs harbor records of 127 endemic species, representing 65% of the 196 endemic butterfly species recorded in Colombia. Most of these endemic species, 93 species, have been recorded in only one NNP, here defined as “unique” species. These species are mainly distributed along the Andes Cordillera. Among all the NNPs, Sierra Nevada de Santa Marta holds the highest number of both total and unique endemic species. Extrapolating this pattern to the broader Andean Biota supports the idea that protected areas play a key role in containing and protecting much of Northern South American endemic biodiversity, underscoring their importance as custodians of biological richness. Full article