Search Results (496)

Seventy 28-day-old weaned barrow piglets (Duroc × Landrace × Large White; 7.2 ± 0.20 kg) were used to determine the effects of 25-hydroxyvitamin D₃ (25-OH-VD₃) combined with phytase and probiotics on calcium and phosphorus metabolism and bone development. Five dietary groups were tested: basal diet + 50 µg/kg 25-OH-VD₃ (CON); basal diet with 17% reduced calcium and phosphorus + 50 µg/kg 25-OH-VD₃ (LCP); LCP + 50 mg/kg phytase (LH); LCP + 10 mg/kg probiotics (LC); LCP + 50 mg/kg phytase + 10 mg/kg probiotics (LHC). The experiment lasted for 31 days, including 3 days adaptation period. Apparent phosphorus digestibility was higher in the LH and LHC groups than in the CON group (p < 0.05). Bone mineral density and calcium content in metacarpal and rib bones were also higher in the LHC group compared with the CON, LCP, LC, and LH groups (p < 0.05). The jejunal mRNA expression of solute carrier family 34 members (SLC34A1, SLC34A2, and SLC34A3) members was higher in the LHC group than the CON, LCP, LC, and LH groups (p < 0.05), while the relative protein expression of the calcium-sensing receptor in the kidneys was lower in the CON group than in the LCP, LH, LC, and LHC groups (p < 0.05). Additionally, supplementation with 25-OH-VD₃, either alone or in combination with phytase and probiotics, was associated with an increased abundance of beneficial gut bacteria. Overall, combined supplementation of 25-OH-VD₃, phytase and probiotics enhanced bone development in weaned piglets fed a low-calcium, low-phosphorus diet by improving calcium and phosphorus utilization and calcium–phosphorus metabolic regulation. Full article

Background/Objectives: Comprehensive knowledge of body composition and bone status across the lifespan is critical for clinical evaluation and public health initiatives. This study aimed to develop age- and sex-specific reference curves for body composition and bone status in a physically active Greek population aged 18–80 using dual-energy X-ray absorptiometry (DXA). A secondary objective was to examine age- and sex-related trends in fat distribution, lean mass (LM), and bone status. Methods: A cross-sectional analysis was conducted on 637 participants (275 men and 362 women). Physical activity was assessed through structured interviews evaluating type, frequency, and intensity, categorized using established guidelines from organizations such as the American Heart Association and World Health Organization. Anthropometric data and DXA scans were utilized to measure parameters including fat mass (FM), LM, and BMD. Participants were stratified into age categories, and percentile curves were generated using generalized additive models for location, scale, and shape (GAMLSS). Results: Among women, body mass increased by 20.9% and body fat percentage rose by 38.3% from the youngest to the oldest age group, accompanied by a 5.7% reduction in bone mineral density (BMD) and an 11.5% decline in bone mineral content (BMC). Men exhibited a 49.1% increase in body fat percentage, with LM remaining stable across age groups. In men, BMD decreased by 1.7%, while BMC showed minimal variation. Notable sex differences were observed in fat redistribution, with android fat (AF) increasing significantly in older individuals, particularly among women, highlighting distinct age-related patterns. Conclusions: This study provides essential reference data on body composition and bone status, emphasizing the need for tailored interventions to address sex- and age-related changes, particularly in fat distribution and bone density, to support improved health outcomes in aging populations. Full article

Background: This study investigated the relationships between hematological and bone metabolism variables in 35 elite female trail runners, focusing on identifying key hematological correlates of bone health. Methods: Forty-four hematological variables, including biochemical, hormonal, metabolic, liver enzyme, and iron profiles, as well as complete blood count and platelet indices, were analyzed. Bone mineral density (BMD) and bone mineral content (BMC) were assessed at multiple skeletal regions via dual-energy X-ray absorptiometry (DXA). A cross-sectional design was employed, utilizing descriptive statistics, correlation analyses, and multiple linear regression to analyze the associations between hematological markers and BMC and BMD. Results: Significant but moderate associations were identified: magnesium consistently emerged as a negatively associated factor, particularly associated with BMC and BMD in the lumbar spine (L1–L4) and whole-body, potentially reflecting hypothesized mineral mobilization during chronic physical stress. Follicle-stimulating hormone showed positive associations with BMD, suggesting a potential protective association in bone turnover regulation. Additionally, calcium and thyroid hormones were linked to regional bone properties, highlighting site-specific skeletal vulnerabilities. Conclusions: These findings suggest a complex interplay between mineral homeostasis and hormonal balance that may be related to skeletal integrity in elite female trail runners. This work provides a foundation for developing evidence-based guidelines to support the health and performance of female endurance athletes. Further research is warranted to confirm these results through longitudinal evaluations. Full article

Improving phosphorus (P) utilization in broilers is crucial for reducing feed costs and environmental pollution. Bone mineralization trait is strongly associated with P utilization in poultry and is thus often used as an alternative trait for evaluating P utilization. Dentin matrix protein 1 (DMP1), an essential matrix protein for bone mineralization and P deposition, has been shown to be actively involved in P utilization in broilers, but the underlying mechanisms remain unclear. The current study aimed to investigate the possible mechanisms whereby DMP1 regulates P utilization of poultry by using gene silencing and overexpression technologies, combined with an in vitro model of primary broiler osteoblasts. The results showed that DMP1 overexpression augmented the P utilization of broiler osteoblasts, characterized by significant increases (p < 0.001) in P utilization rate, mineralization formation, alkaline phosphatase activity, and bone gla protein content. Meanwhile, DMP1 overexpression effectively (p < 0.05) activated the focal adhesion kinase (FAK) signaling, along with obvious (p < 0.01) decreases in fibroblast growth factor 23 (FGF23) expression and production. In contrast, DMP1 silencing reversed (p < 0.05) the above effects. Consistently, FAK activation promoted (p < 0.05) P utilization accompanied by remarkable (p < 0.05) decreases in FGF23 expression and production. Furthermore, gain- and loss-of-function assays demonstrated that a high level of FGF23 contributed to impaired P utilization, while a low level was beneficial. Interestingly, blocking FAK signaling not only recovered (p < 0.05) the FGF23 expression and production in DMP1 overexpressed cells but also obviously (p < 0.05) weakened their P utilization. These findings indicate that DMP1 inhibits FGF23 expression by activating FAK, thereby contributing to P utilization in broiler osteoblasts. They reveal a novel DMP1-FAK-FGF23 regulatory axis in broiler osteoblasts and provide a potential target for improving P efficiency in poultry. Full article

Background/Objectives: This cross-sectional study aimed to evaluate the relationship between nutritional intake (energy, protein, calcium, number of meals, number of dairy products) and other factors (physical activity, past fractures, body composition) with forearm bone parameters in adolescent boys with myelomeningocele (MMC). Methods: This study included 63 boys with MMC aged 11.9 ± 1.8 years, 30 active boys with MMC and 33 inactive boys with MMC. Bone mineral density (BMD) and bone mineral content (BMC) in the distal (dis) and proximal (prox) parts of the forearm were measured by densitometry. Diet was assessed using an FFQ and 24 h dietary recalls. Energy, protein, and calcium intake were calculated using the Diet 6.0 software. Data were collected on past fractures and physical activity (PA min/day). Results: The active MMC group, compared to the inactive group, had significantly higher BMD dis and prox, BMC dis, and Z-scores (Hedges’ g: medium effect). Significant relationships between BMD dis were demonstrated with the number of dairy products (n/day) (F = 6.66; η² = 0.116) and protein intake (g/day) (F = 15.27; η² = 0.230). BMC dis was affected only by PA (min/day) (F = 9.80; η² = 0.161). The parameters affecting BMD prox were the number of dairy products (n/day) (F = 9.95; η² = 0.163) and protein (g/day) (F = 12.95; η² = 0.202). BMC prox was affected only by PA (min/day) (F = 4.39; η² = 0.079). Conclusions: Overall, bone health in boys with MMC appears to be primarily influenced by nutritional factors—particularly dairy intake and protein—as well as by physical activity. These results underscore the need for early nutritional screening and further research on additional bone-related dietary components to optimize nutritional recommendations for this population. Full article

The consumption of water with very low mineral content (W-VLMC; water with total dissolved solids below 50 mg/L), despite limited and inconsistent evidence and the resulting knowledge gaps, has not been associated with health risks for the general population. However, certain population subgroups (those eating very unbalanced diets or avoiding certain foods, engaged in prolonged periods of fasting, and/or doing prolonged or strenuous exercise as well as pregnant or breastfeeding women) should be mindful of maintaining sufficient intake of all essential minerals through their food if regularly using this type of water as their main beverage. The rapid expansion of water filtration systems—often producing W-VLMC—creates a timely and valuable opportunity to advance research on the health implications of W-VLMC intake. As these systems become increasingly common in educational settings and homes, children represent a subgroup experiencing rising exposure to W-VLMC. Additional studies are needed to assess the health effects of such exposure from early childhood. A complementary yet contrasting perspective is that the use of water intended for human consumption—with stringent quality control standards—and natural mineral waters—inherently pure, thus eliminating the need for filtration—with higher mineralization in both types of water, may provide an additional dietary source of essential minerals, especially for all the population subgroups mentioned above. Full article

Background/Objectives: Early-stage breast cancer treatments adversely affect components of health-related fitness (HRF) important for treatment tolerability, recovery, and long-term outcomes. Few studies have examined cancer treatment modality-specific effects on HRF. We examined associations of breast cancer treatment modalities, regimens, and combinations with one-year changes in HRF. Methods: Newly diagnosed early-stage breast cancer patients were recruited between 2012 and 2019 for the Alberta Moving Beyond Breast Cancer (AMBER) cohort study. HRF assessments were completed within 90 days of diagnosis and at one year, including cardiorespiratory fitness, muscle strength and endurance, and body composition. Analysis of covariance was used to test whether HRF changes differed between treatment modalities, regimens, and combinations. All tests were 2-sided. Results: A total of 1350 participants (mean [SD] age, 55.6 [10.7] years) were included. Women who received chemotherapy (n = 797; 59%) experienced statistically significant smaller increases in upper body strength (−1.7 kg, 95% confidence interval [CI]: −3.0 to −0.5), greater declines in lower body endurance (−118.0 kg, 95%CI: −216.6 to −19.3), and greater declines in total lean mass (−0.7 kg, 95%CI: −1.1 to −0.3), bone mineral density (−0.01 g/cm², 95%CI: −0.02 to 0.00), and bone mineral content (0.04 kg, 95%CI: −0.06 to −0.02). Other treatment modalities were modestly and inconsistently associated with HRF changes. Treatment combinations that included chemotherapy had the most negative impact on cardiorespiratory fitness and body composition. Conclusions: Chemotherapy—either alone or in combination with other treatments—had the largest and broadest negative impact on HRF recovery in early-stage breast cancer at one-year follow-up. Full article

Bone is one of the most important organs of mammals, consisting of collagen and apatite. Various diseases, such as osteoporosis, can affect the components of bone tissue, their chemical composition and bone ultrastructure, which leads to changes in properties. In this paper, the effect of initial osteoporosis on the chemical composition of bone apatite and the ultrastructure of bone tissue from a mineralogical point of view is analyzed using rat femurs as an example. The chemical composition of bone apatite was studied using SEM, EDS and FTIR-ATR spectroscopy. The bone ultrastructure was examined using a transmission electron microscope. An increase in the content of carbonate ion in the position of the phosphorus group and a change in the orientation of apatite crystals inside mineral plates were revealed against the background of initial osteoporosis, which can affect not only the mechanical properties of bone, but also the stability of apatite under biological conditions. Full article

The growth and development of skeletal structures in young animals are important for their overall health and productivity. Deciphering variations in bone growth among pig breeds can optimize breeding strategies and improve animal welfare. This study explored differences in the bone growth and development of different pig breeds at the early growth stages. A total of 120 newborn piglets from Taoyuan black (TB), Xiangcun black (XB), and Duroc piglets, from 30 sows (litter sizes 9–11), were selected to assess their bone growth and development at four different ages (including at 1, 10, 21, and 24 days old). The results showed that the bone length and weight of femur, tibia, rib, and lumbar vertebrae among different pig breeds increased with age. Compared to Duroc piglets, XB and TB piglets exhibited a shorter bone length and lighter bone weight of those four bones at different ages (p < 0.05). XB piglets showed shorter bone length of femur, tibia, and lumbar vertebrae at weaning (21 days old), and a lighter bone weight and lower bone mineral content of those three bones at weaning and three days after weaning (24 days old) compared to TB piglets (p < 0.05). Bone breaking load increased with age, with Duroc piglets exhibiting higher bone breaking load at 10 days old (p < 0.05). Bone mineral density, ash content, and Ca and P deposition varied by breed and age, and TB piglets had a higher bone mineral density at 21 days old compared to XB piglets. In summary, Chinese native piglets have slower bone growth than Duroc piglets, but TB piglets exhibit higher mineral deposition compared to XB piglets. Full article

Calcium silicate-based cement is commonly used for bone repair and regeneration. Current research focuses on developing innovative antibacterial materials with radiopacity, which is essential for ensuring successful clinical outcomes in procedures like vertebroplasty and endodontic treatments. Strontium (Sr) has emerged as a powerful additive, stimulating bone formation and inhibiting bone resorption. In this study, we evaluated the impact of varying levels of Sr—5, 10, and 20 mol% (designated as CSSr5, CSSr10, and CSSr20) on critical attributes of bone cement, including radiopacity, setting time, in vitro bioactivity, antibacterial efficacy, and osteogenic activity. The findings indicated that as the Sr content increased, the setting time and radiopacity of the cement increased. Remarkably, the cement formulations containing over 10 mol% Sr achieved radiopacity values surpassing the 3 mm aluminum threshold mandated by ISO 6876:2001 standards. Furthermore, incorporating Sr significantly improved MG63 cell attachment, proliferation, differentiation, and mineralization, while also boosting antibacterial properties in a dose-dependent manner. After 48 h of inoculation with E. coli or S. aureus, the CSSr10 and CSSr20 cements showed a bacteriostatic ratio exceeding 1.7 or 2 times that of the control without Sr. In conclusion, the CSSr10 cement could be a promising bone filler, exhibiting favorable setting time, radiopacity, antibacterial ability, and osteogenic activity. Full article

Background: We evaluated certain factors relative to onion (Allium spp.) and onion-derived polyphenols. Methods: We searched PubMed, Scopus, and Google Scholar from 2010 to 20 October 2025 without language limits. Eligible designs comprised of in vitro, animal, and human studies assessing onion, onion extracts, or isolated onion-derived polyphenols with bone outcomes. Two reviewers independently screened, extracted, and assessed risk of bias (RoB 2 for RCTs; SYRCLE for animals). Results: We included X studies (a in vitro, b animal, and c human RCTs; N samples). In vitro limitations included the following: heterogeneous models, short human follow-up, small samples, and moderate-to-serious risk of bias in animals. Reports included improvements in bone mineral density (BMD), bone mineral content (BMC), bone turnover markers, and osteoclast/osteoblast activity. Onion extracts consistently inhibited RANKL-induced osteoclastogenesis while sparing osteoblast function. In animals, onion or quercetin/kaempferol preserved BMD and improved mineral content and (in several models) fracture healing. In one small RCT of onion juice (8 weeks), antioxidant status improved with a trend to attenuated BMD loss; a resveratrol RCT was excluded/included as a benchmark. Conclusions: Pre-clinical evidence suggests anti-resorptive and osteogenic effects of onion-derived polyphenols; human evidence is limited and characterized by low certainty. Longer RCTs are needed to define effective doses and clinical relevance. Full article

Background: Muscle and bone show reciprocal interactions and are associated in a muscle-bone unit. The muscle-bone unit has been investigated to a very limited extent in soccer players. The objective of this work was to investigate in detail the muscle-bone unit in male youth elite soccer players. Methods: Bone mineral and lean mass were measured with dual-energy X-ray absorptiometry (DXA). The functional muscle-bone unit (fMBU) and the muscle-to-bone ratio (MBR) were calculated from the DXA output in a sample of players aged 14–19 (n = 193) playing in the youth squads of an Italian Serie A team. Results: Statistically significant (p < 0.05) correlations were found between lean mass variables and bone mineral content and density, also after adjusting for age, body mass, stature, maturity, and ethnicity (White/Black). fMBU and MBR were statistically significantly associated with age, body mass, stature, maturity, and ethnicity. Linear regression showed that body lean mass was the strongest predictor for bone mineral content and density. Age was a statistically significant predictor for fMBU and MBR. Playing position did not show any statistically significant relationship with bone mineral content and density, as well as fMBU or MBR. Centiles for fMBU and MBR were calculated as a reference. Conclusions: This work is the first detailed characterization of the muscle-to-bone relationship in soccer players. It is expected to be of use for sport scientists and the wide community of sportsmen and professionals involved in soccer. Full article

This study systematically examined the effects of Tai Chi exercise on bone health in menopausal women, with subgroup analyses of potential moderators. A systematic search was conducted across nine databases (PubMed, Web of Science, Cochrane Library, EBSCO-Medline, EBSCO-Sportdiscus, Embase, CNKI, VIP and Wanfang Data) on June 1 and updated on 14 September 2025 to identify controlled trials evaluating perimenopausal or postmenopausal women. A three-level meta-analysis was performed to pool effect estimates, reported as standardized mean differences (SMDs), with heterogeneity further explored through subgroup analyses. Across 16 studies involving 1091 participants aged 49–64 years, Tai Chi interventions led to significant improvements in bone health. Training protocols ranged from 6 to 104 weeks, with sessions lasting 30 to 90 min. Bone mineral density (BMD) improved significantly at the femoral neck (SMD = 0.50), greater trochanter (SMD = 0.61), and lumbar spine L2–L4 (SMD = 0.81), with stronger effects observed in perimenopausal women. Bone mineral content (BMC) also increased significantly in menopausal women (SMD = 1.63, I² = 91.46%), although heterogeneity was substantial, and no significant differences were detected in subgroup moderators. In contrast, no significant effects were found for bone mineral metabolism (p = 0.38) or bone turnover markers (p = 0.25). According to GRADE assessments, the certainty of evidence ranged from low to moderate across these outcomes. In conclusion, while Tai Chi has been shown to improve BMD and BMC in menopausal women, the relatively high heterogeneity observed for BMC necessitates cautious interpretation of these particular outcomes. In contrast, no statistically significant effects were observed on bone mineral metabolism (BMM) and bone turnover markers (BTMs). Notably, given the significant differences observed between perimenopausal and postmenopausal women, future well-designed studies that stratify participants by menopausal status and possess adequate statistical power are needed to further explore the potential differential effects of Tai Chi on bone health. Full article

Objectives: The study aimed to determine the effect of manganese (Mn) exclusion from the dietary mineral mixture and the dietary replacement of the recommended level of MnCO₃ with Mn₂O₃ nanoparticles (Mn₂O₃NPs) on the Mn biodistribution and the femur histology. Methods: The experiment was conducted on twenty seven Wistar rats divided into three groups (n = 9): a control group receiving the recommended level of Mn (65 mg/kg) in standard form (MnCO₃); a manganese deficient group (Mn deprived from dietary mineral mixture), and a group receiving diet supplemented Mn₂O₃NPs (65 mg/kg) instead of MnCO₃. During the 12-week experiment, a balance test was performed. After the experiment period, blood and femur were collected from sacrificed rats. The content of Mn in water, diet, urine, feces, plasma, and femur was measured. Results: In the Mn-deficient rats, a reduction in Mn intake and excretion, Mn retention index, and blood Mn level, but an increase in Mn digestibility index was noted. In rats supplemented with Mn₂O₃NPs, Mn intake and excretion and blood Mn levels were decreased, while Mn retention and digestibility indexes were increased. In both experimental groups, deterioration of femur morphology was noted, but these changes were more severe in the Mn-deficient group. Conclusions: The obtained research results indicate that manganese deficiency significantly disturbed the biodistribution of this element and led to the deterioration of the architecture and histological parameters of the femur, emphasizing the key role of manganese in maintaining bone homeostasis. It has also been shown that replacing MnCO₃ with Mn₂O₃NPs allows the maintenance of the correct Mn level in the femur but causes unfavorable changes in its morphology. Full article

Background/Objectives: While oral nutritional supplements (ONSs) are known to support general growth in undernourished children, their specific effects on body composition and bone health remain underexplored. This manuscript evaluates the impact of ONS combined with dietary counselling (DC) on these outcomes in Indian children aged 3 to 6.9 years at nutritional risk, within the framework of a larger randomized controlled trial assessing multiple growth parameters. Methods: This prospective, randomized controlled trial was conducted among Indian children, both male and female participants with picky eating habits and at risk of undernutrition, aged 3 to 6.9 years (height-for-age and weight-for-height below the 25th percentile per WHO Growth Standards and Growth Reference). Participants were randomized in a 1:1 ratio to receive either ONS + DC or DC alone for 6 months. Body composition, bone mineral density (BMD), and bone mineral content (BMC) were measured using dual-energy X-ray absorptiometry (DXA) at baseline and after 6 months. Group differences were analyzed to evaluate intervention effects. Results: A total of 223 children were enrolled and randomized. At 6 months, the test group showed significantly greater improvements in BMD (0.023 g/cm²) compared to the control (0.017 g/cm²; p = 0.004), and a greater gain in BMC (36.60 g vs. 28.48 g; p =0.0007). Lean mass increased significantly more in the test group (926.33 g) versus the control (801.48 g; p = 0.0401). Fat mass showed a numerical reduction in the test group (−171.42 g) compared with the control group (−114.60 g), although this difference was not statistically significant. Conclusions: These findings highlight the potential of targeted nutritional interventions to favorably improve body composition and bone health during critical growth periods in undernourished children, offering a promising approach to address early-life nutritional deficits with lasting health benefits. Full article