Search Results (86)

Background/Objectives: Liquid profiling of molecular and epigenetic markers in bodily fluids is an expanding field of cancer biomarker research. Recent research activity also reveals the human satellite 2 (HSAT2) repetitive element cell-free DNA (cfDNA) as a potential cancer biomarker. Based on our recent results from targeted sequencing of HSAT2 cfDNA, we tested whether a specific HSAT2 sequence (e.g., 95 bp-HSAT2) shows greater cancer enrichment than 114 bp-SAT2, from which it derives, in patients with colon cancer. Methods: By comparing the ratio of 114 bp-HSAT2 to 95 bp-HSAT2, we investigated the increased cancer enrichment of 95 bp-HSAT2 in cfDNA samples obtained from plasma DNA extraction and a hybridization capture assay, in which HSAT2 sequences were captured from plasma using a biotin-labeled probe, in samples from colon cancer patients (n = 60) and polyp-controls (n = 60), and polyp-free controls (n = 60). Results: A correlation analysis between Ct values from DNA extraction and the hybridization capture assay for both 95 bp- and 114 bp-HSAT2 showed a positive correlation in patients with colon cancer and control subjects, indicating that the hybridization capture assay provides HSAT2 levels comparable to those obtained by DNA extraction. With both approaches, we found a lower 114 bp-HSAT2 to 95 bp-HSAT2 ratio in patients with colon cancer than in the control groups. The median ratio of extracted DNA was 62, 78, and 79 in patients with colon cancer, polyp-controls (p = 0.23), and polyp-free controls (p = 0.067), respectively. Capture assay values were 49, 87, and 64 in patients with colon cancer, polyp controls (p = 0.016), and polyp-free controls (p = 0.19), respectively. Even though statistical significance was not achieved in some comparisons, these results suggest that 95 bp-HSAT2 is more abundant in the blood of patients with colon cancer than 114 bp-HSAT2 in non-malignant patients. Conclusions: To our knowledge, this is the first study to conduct a hybridization capture assay using a biotinylated probe as a feasible approach for targeted enrichment of cfDNA from plasma. Our results confirm the outcomes of our recent article based on targeted sequencing and reveal that some specific HSAT2 sequences may exhibit increased cancer abundance. Full article

Swaddling, known in Quechua as qillpu, is an ancestral practice still in use in Andean communities, linked to cultural conceptions of health, protection, and appropriate ways of raising babies. The objective of the research was to analyze the sociocultural meanings and practices associated with qillpu, as well as the tensions that arise in relation to conventional medicine in the field of childhood. A qualitative ethnographic approach was used, involving in-depth interviews with mothers, participant observation, and interpretive analysis of the discourses collected in Andean communities. The results show that swaddling is perceived as a comprehensive care strategy that promotes rest, bodily symmetry, and the physical and spiritual protection of the child. The testimonies legitimize its use in the face of accusations of abuse, emphasizing its temporary and functional nature. It is concluded that the qillpu maintains a solid symbolic and practical relevance, and therefore its analysis requires an intercultural dialogue that articulates ancestral knowledge with biomedical approaches. Full article

Based on extensive ethnographic fieldwork conducted in Mianning County, Liangshan Yi Autonomous Prefecture between 2023 and 2024, this paper analyzes the “xiō bū” (ꑭꁮ) ritual of the Liangshan Yi people. Framed within contemporary approaches to religious anthropology and social memory theory, the study explores how this ritual constructs Yi ecological ethics, social integration, and cultural identity through nature worship, ancestral spirit beliefs, and ritual practices. The ethnographic evidence reveals that the “xiō bū” ritual, by designating wooded mountains as sacred space and performing sacrifices to nature deities and ancestral spirits, integrates “humans—nature—ancestors” into a symbiotic system of the “community of life.” This reflects the Yi people’s relational ontology and embedded ecological knowledge. The sacrificial offerings, shared meals, and purification practices in the ritual not only reinforce reverence for nature through symbolic acts but also unify the community through Durkheimian “collective effervescence,” thereby restoring the community’s spiritual order. As a carrier of social memory, the “xiō bū” ritual, through epic chanting, symbolic performances (such as clothing, ritual implements), and bodily practices (like the ritual specialist’s movements), embeds individual memories into the collective historical narrative of the group, dynamically constructing the cultural boundaries of the “Yi” people. The ritual specialists (Bimo or Suni), as intermediaries of knowledge and power, maintain religious authority through bricolage-like symbolic reorganization and foster the creative transformation of tradition in response to the challenges of modernity. The study further reveals that while the ritual faces challenges in the contemporary context, such as secularization and population mobility, it continues to activate ethnic identity by simplifying rituals, preserving core symbols, and coupling with ecological discourses, offering a model for the modern adaptation of traditional religions. This paper argues that ritual studies should engage with contemporary theoretical approaches like the ontological turn, focus on the agency of individuals, and reflect on the insights traditional knowledge systems offer in the face of globalization and ecological crises. Full article

Background: Ostomy care consultations are essential for promoting patient autonomy and quality-of-life. The integration of innovative technologies may enhance health education and support effective self-care among ostomized patients. Objective: To evaluate the impact of a nursing-led health education intervention supported by smart-glasses on the quality of life of ostomized patients. Methods: A pre–post quasi-experimental design was employed with 14 patients who had undergone digestive surgery resulting in an ostomy. The intervention consisted of a single 60-min session comprising three phases: (1) assessment of baseline knowledge on ostomy management, (2) personalized feedback, and (3) a hands-on workshop using Vuzix© smart-glasses to demonstrate ostomy care techniques. Quality of life was assessed using the SF-36 questionnaire before and after the intervention. Results: The intervention significantly improved overall SF-36 scores, with notable advancements in emotional role (78.57 ± 36.06 to 97.44 ± 9.25, d = 10.54), mental health (79.14 ± 20.10 to 87.38 ± 13.94, d = 6.27), and vitality (69.29 ± 20.56 to 71.15 ± 16.98, d = 4.19). Social function remained high throughout the study, while bodily pain showed a slight decline. A strong correlation (ρ = 0.923, p = 0.001) was observed between pre- and post-intervention quality of life scores. Conclusions: The findings suggest that integrating smart-glasses into nursing-led health education may enhance the quality of life and self-care capabilities of ostomized patients. However, the small sample size, lack of a control group, and exploratory nature of the study limit the generalizability of the results. Further research is needed to validate these findings in larger, controlled trials. Full article

Endometriosis is a common gynecological pathology, with an incidence of nearly 10% in patients of reproductive age, and is still underdiagnosed. A thorough and well-spread diagnostic study of endometriosis based on epigenetic factor dysregulation can highlight potential areas for improvement. To quantify the potential and utility of non-invasive tools in the early diagnosis of endometriosis, an overview of current knowledge on epigenetic factors, based on DNA and RNA, is presented. Among these tools, it is important to highlight the role of miRNAs (microRNAs), cfDNA (cell-free DNA), and rRNAs (ribosomal RNAs), which are small molecules involved in endometriosis and numerous other pathologies. To evaluate their potential and utility in endometriosis, a salivary miRNA diagnostic test was conducted, the cfDNA methylation patterns of fragmented DNA circulating in bodily fluids (e.g., plasma) were analyzed, and cervical and uterine microbiomes were profiled for bacterial rRNA in patients with clinical suspicion of incipient endometriosis. Specific molecular profiles associated with endometriosis were analyzed. The first profile, a 109-miRNA saliva signature, was validated as a product of miRNA biomarkers and artificial intelligence modeling. In addition, peripheral blood cfDNA methylation biomarkers were identified by investigating nine genes in a molecular signature that requires validation. A profile was also obtained from cervical swabs and uterine washes, including molecular analysis of 16S rRNA amplicon sequencing to evaluate alterations in the cervical bacterial community. This review aims to optimize the integration of a non-invasive diagnostic tool for early endometriosis diagnosis. Genetic biomarkers can be correlated with clinical factors to improve diagnostic accuracy. Of the assessed diagnostic tools, salivary miRNA tests, a peripheral blood cfDNA methylation biomarker, and a microbiome rRNA signature may be useful for early diagnosis of endometriosis, as well as, implicitly, therapeutic attitude and follow-up. Full article

As prosthetic technologies become increasingly data-rich and embedded in care systems, traditional human-centred approaches often fall short of addressing evolving use realities. This paper contributes an applied computing framework that enables semantic reasoning and data-driven adaptation within prosthesis aftercare. We present an ontology-driven, cyber–physical prosthesis service system designed to enable personalised and adaptive care. Implemented through the Adaptive Prosthesis Life-Cycle Service System (adProLiSS) framework and demonstrated via a smart prosthesis prototype, the system treats the prosthesis as a semi-autonomous actor within an emotionally responsive and semantically mediated ecosystem. The proposed architecture integrates sensor data acquisition, ontology-based knowledge representation, and semantic reasoning to enable context-aware decision support and adaptive personalisation. A layered cyber–physical infrastructure, comprising embedded sensors, semantic reasoning, and user feedback through a digital twin interface, supports personalised aftercare, cross-disciplinary collaboration, and reflective design engagement. Evaluation with 26 participants across clinical, engineering, and user groups confirmed the system’s value in enhancing functionality, reducing downtime, and supporting emotional well-being. By positioning ontologies as both computational enablers and design support mechanisms, this research contributes a practical and scalable model for prosthetic service systems that adapt across bodily, emotional, and ecological dimensions, advancing more responsive and consequence-aware care practices. Full article

While existing research highlights a positive association between computational thinking (CT) and self-regulation (SR) skills, limited attention has been given to the embodied and social processes within CT activities that support young children’s executive functions (EFs)—key components of SR. This study investigates how preschoolers develop basic and higher-order EFs, such as focused attention, inhibitory control, causal reasoning, and problem-solving, through their engagement with a tangible programming toy in teacher-guided small groups in a university-affiliated preschool. Informed by a we-syntonicity framework that integrates Papert’s concepts of body/ego syntonicity and Schutz’s “we-relationship”, we conducted a multimodal microanalysis of video-recorded group sessions. Our analysis focuses on two sessions, the “Obstacle Challenge” and “Conditionals”, featuring four excerpts. Findings reveal that children leverage bodily knowledge and empathy toward the toy—named Rapunzel—to sustain attention, manage impulses, reason about cause-effect, and collaborate on problem-solving. Three agents shape these processes: the toy, fostering collective engagement; the teacher, scaffolding learning and emotional regulation; and the children, coordinating actions and sharing affective responses. These findings challenge traditional views of SR as an individual cognitive activity, framing it instead as an embodied, social, and situated practice. This study underscores the importance of collaborative CT activities in fostering SR during early childhood. Full article

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder and one of the most pressing global health challenges. Increasing evidence highlights oxidative stress as a key factor in its pathogenesis, contributing to amyloid-β accumulation, tau hyperphosphorylation, neuroinflammation, and mitochondrial dysfunction. Oxidative stress markers, detected in the bodily fluids of AD patients, are considered promising diagnostic and prognostic tools. Despite extensive research, currently available therapies remain largely symptomatic, which emphasizes the need to develop novel, disease-modifying strategies. The aim of this review is to summarize current knowledge on the role of oxidative stress in the pathogenesis of AD and to evaluate therapeutic approaches aimed at its reduction. We discuss molecular mechanisms linking reactive oxygen species to neurodegeneration and present pharmacological strategies such as monoamine oxidase inhibitors and multifunctional agents, as well as natural antioxidants, dietary interventions, and novel therapeutic technologies. We pay particular attention to their efficacy, limitations, and translational challenges. A more profound understanding of oxidative stress-related mechanisms may facilitate the development of combined antioxidant, anti-inflammatory, and neuroprotective approaches, offering new perspectives for delaying disease progression and improving patient outcomes. Full article

There is limited knowledge about the benefits of functional textile in arthritis management. This study aimed to evaluate the effect of wearing functional socks in patients with rheumatoid or psoriatic arthritis. Patients were randomized into an experimental group (n = 23) and control group (n = 18). The intervention involved wearing functional textile socks for 12 weeks. Sock composition was analyzed using X-ray fluorescence spectrometry and scanning electron microscopy. Outcome measures included the Numeric Rating Scale, Health Assessment Questionnaire–Disability Index (HAQ-DI), and RAND-36 (Estonian version). At week 12, the experimental group showed significantly lower metatarsophalangeal and toe joint pain (p = 0.001), stiffness (p = 0.005), and ankle stiffness (p = 0.017) scores than the control group. Improvements were also observed in HAQ-DI reaching (p = 0.035) and activity (p = 0.028) scores. RAND-36 scores were higher in physical functioning (p = 0.013), social functioning (p = 0.024), and bodily pain (p = 0.006). Role limitations due to physical problems improved in the experimental group but worsened in the control group (p = 0.029). In conclusion, wearing functional socks led to some statistically significant improvements in foot and ankle pain and stiffness, physical function, and health-related quality of life. However, the effect sizes were small, and the clinical relevance of these findings should be interpreted with caution. Full article

This paper discusses the role of energy and movement in facilitating experiences of well-being from an anthropological perspective that considers energy as a situated form of knowledge. Drawing on fieldwork among Core Energetics (CE) and 5Rhythms (5R) groups, I examine how the circulation of energy is achieved through bodily movements and dances, attention to the senses, somatic self-cultivation, and deep experiences of consciousness. Focus is hold on the ritual elements, including a specific spacetime framework and intersubjective exercises that facilitate energetical experiences that renew the subjective and intersubjective relationship to the self in a restorative way. I argue that mind–body–energy groups conflate two Western sources: on the one hand, the legacy of early Western medical offshoots that have been marginalized by mainstream biomedicine, and on the other, discursive references to contemporary interpretations of quantum physics. Full article

Circulating free DNA (cfDNA) is genetic material released from various cells into bodily fluids. Among its fractions, circulating tumor DNA (ctDNA) originates from tumor cells and reflects their genetic material, including mutations and epigenetic changes. Methods commonly employed for detecting ctDNA in blood include next-generation sequencing (NGS) and various types of PCR. The presence of ctDNA can be utilized in liquid biopsies for many diagnostic purposes related to various cancers. It is a minimally invasive method of sampling molecular compounds from tumor cells. In this paper, we focus on current knowledge regarding the liquid biopsy of blood ctDNA in the context of lung cancer, one of the leading causes of cancer-related mortality. Currently, as a clinically approved method, liquid biopsy serves as a complementary technique in NSCLC diagnostic and genetic profiling. Other applications of liquid biopsy that are still being investigated include the detection of minimal residual disease (MRD) after curative treatment and response monitoring to systemic treatment. This review discusses current and future potential directions for the development and implementation of ctDNA for patients with NSCLC. Full article

Recent cognitive literary studies of fiction have begun to reveal patterns in the ways authors engage readers’ bodily and environmentally grounded imaginations. This study brings fiction writers’ craft knowledge into conversation with neuroscientific, cognitive, and literary studies of multimodal imagery and other embodied responses to fiction reading. Developed through years of literary experiments, craft knowledge involves using language not just to engage readers’ senses but to broaden their understandings of how senses work. A close analysis of Edwidge Danticat’s craft techniques in Breath, Eyes, Memory (1994) affirms some recent literary and scientific findings on how language can activate readers’ sensory and motor systems. Danticat’s cues to readers’ imaginations present a relational, environmentally engaged kind of sensorimotor experience that may widen scientific understandings of how sensory and motor systems collaboratively ground cognition. By helping diverse readers imagine a young Haitian American woman’s movements, sensations, and emotions, Danticat’s craft also does political work, depicting the inner lives of characters under-represented in widely published fiction. Full article

This study examines the effects of implementing a movement-integrated instruction (MII) program in third-grade mathematics classes with a focus on students’ mathematical learning outcomes and physical literacy development. The program was designed using the Analysis, Design, Development, Implementation and Evaluation (ADDIE) instructional model and was implemented in a public elementary school in South Korea. While the primary instructional emphasis was placed on improving mathematical concept comprehension and problem solving, the study also evaluated outcomes in three core areas of physical literacy: physical competence, motivation and confidence, and knowledge and understanding of physical activity. A descriptive qualitative approach was adopted and supplemented with quantitative data. The data sources included classroom observations, learning artifacts, teacher reflections, semi-structured interviews, and structured student surveys. The results showed that 82.6% of students reported improved bodily control and coordination, while 75.4% indicated that they used skills acquired through physical education (PE) to solve math problems. Student work demonstrated an increasing use of multi-step reasoning, diagrammatic representations, and contextual explanations, suggesting that embodied learning reinforces both cognitive engagement and physical development. Although challenges related to time, space, and varying motor abilities were encountered, they were addressed through interdisciplinary integration and differentiated instructional strategies. This study provides empirical support for MII as a pedagogical model that effectively bridges academic learning and physical development, and offers practical recommendations for broader applications in elementary education. Full article

This study assessed and compared the postmortem concentrations of 6-monoacetylmorphine [6-MAM] and 6-acetylcodeine [6-AC], morphine, and codeine in various tissues and fluids from 52 postmortem cases related to heroin use. Samples were received at the Poison Control and Forensic Chemistry Center in Jeddah, Saudi Arabia, and analyzed using liquid chromatography–mass spectrometry. Descriptive and inferential statistics, including median, range, variability, and outliers, were used for analysis. The results showed significant variability in heroin and metabolite concentrations across different fluids and tissues. Tissue specimens were analyzed in 38 cases (73%), with 50% of cases exhibiting putrefaction. Blood and tissue samples were available in 39 cases, highlighting the need for alternative specimens in challenging cases. Notably, heroin metabolites were detected in unique matrices, such as nasal swabs, bladder tissues, lung tissues, and small intestine tissues, underscoring the potential of these samples in forensic investigations, especially when traditional bodily fluids are unavailable or compromised. These findings suggest that environmental factors, timing of substance use, and postmortem changes influence substance distribution, emphasizing the need to consider the location of death when interpreting toxicological results for accurate forensic analysis. This study provides valuable insights into the distribution, correlation, and significance of heroin and its metabolites in postmortem samples, aiding the confirmation of heroin overdose. These findings contribute to the limited data on postmortem cases in the Middle East and North Africa, particularly Saudi Arabia, supporting efforts to curb drug abuse in this region. This knowledge can inform public health strategies and forensic practices, ultimately aiding efforts to address and mitigate drug abuse. Full article

This paper represents an attempt to investigate some aspects of Vedic religiosity, as represented in the R̥gveda (R̥V), in comparison with the Pāli Canon on the themes of cognition, contemplative practice, and the theory of knowledge. It aims to argue that the metaphors in R̥V, for instance, fire, the chariot, the yoke, light, and the ocean, bear proto-Buddhist ideas that have influenced Buddhist meditative practices. These metaphors reflect a theory of knowledge and cognition that shares certain features with the Pāli Canon. The Vedic seer, the figure around whom this discussion revolves, is a forerunner of the Buddhist practitioner, and the themes of surmounting ignorance and gaining knowledge are common to both of them. The article identifies two major metaphorical fields: one related to knowledge and cognition and the other related to contemplative practice and liberation. The analysis investigates how Vedic metaphors represent an early conceptualization of “technics”, both bodily and contemplative. It underlines similarities between Vedic contemplative exercises, usually understood as a form of prayer, and Buddhist meditation. While the Vedic tradition is focused on divine association, the Buddhist framework reinterprets these ideas within a human-centered perspective. The transformation of Vedic metaphors into Buddhist concepts shows an intricate dialogue rather than an absolute rejection of Vedic traditions. Full article