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19 pages, 1651 KiB  
Article
Genetic Evaluation of Growth Traits in Black-Boned and Thai Native Synthetic Chickens Under Heat Stress
by Wootichai Kenchaiwong, Doungnapa Promket, Vatsana Sirisan, Vibuntita Chankitisakul, Srinuan Kananit and Wuttigrai Boonkum
Animals 2025, 15(15), 2314; https://doi.org/10.3390/ani15152314 - 7 Aug 2025
Abstract
Heat stress is a critical constraint to poultry production in tropical regions, where the temperature–humidity index (THI) frequently exceeds thermoneutral thresholds. Despite growing interest in climate-resilient livestock, limited research has explored the genetic sensitivity of local chicken breeds to increasing THI levels. This [...] Read more.
Heat stress is a critical constraint to poultry production in tropical regions, where the temperature–humidity index (THI) frequently exceeds thermoneutral thresholds. Despite growing interest in climate-resilient livestock, limited research has explored the genetic sensitivity of local chicken breeds to increasing THI levels. This study aimed to evaluate the genetic effects of increasing THI on growth performance traits in two tropical chicken breeds. The data included body weight (BW), average daily gain (ADG), and absolute growth rate (AGR) from 4,745 black-boned and 3,001 Thai native synthetic chickens across five generations. Growth data were collected from hatching to 12 weeks of age, whereas temperature and humidity were continuously recorded to calculate daily THI values. A reaction norm model was used to estimate genetic parameters and rate of decline of BW, ADG, and AGR traits under varying THI thresholds (THI70 to THI80). Results indicated that the onset of heat stress occurred at THI72 for black-boned chickens and at THI76 for Thai native synthetic chickens. Heritability estimates for BW, ADG, and AGR decreased as the THI increased in both chicken breeds. However, the Thai native synthetic chickens consistently exhibited higher genetic potential across all THI levels (average heritability: BW = 0.28, ADG = 0.25, AGR = 0.36) compared to the black-boned chickens (average heritability: BW = 0.21, ADG = 0.15, AGR = 0.23). Under mild heat stress (THI72), black-boned chickens showed sharp declines in all traits (average reduction in BW = −10.9 g, ADG = −0.87 g/day, AGR = −3.20 g/week), whereas Thai native synthetic chickens maintained stable performance. At THI76, both breeds experienced significant reductions, particularly in males. Estimated breeding values (EBVs) for AGR decreased linearly with THI, though Thai native synthetic chickens showed greater individual variability, with some birds maintaining stable or positive EBVs up to THI80—suggesting the presence of heat-resilient genotypes. In conclusion, Thai native synthetic chickens demonstrated superior thermotolerance and genetic robustness under increasing THI conditions. The identification of breed-specific THI thresholds and resilient individuals provides novel insights for climate-smart poultry breeding. These findings offer valuable tools for genetic selection, environmental management, and long-term adaptation strategies in response to global climate change. Full article
(This article belongs to the Section Poultry)
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13 pages, 1560 KiB  
Article
Detection of the Early Sensitive Stage and Natural Resistance of Broad Bean (Vicia faba L.) Against Black Bean and Cowpea Aphids
by Fouad Meradsi, Adel Lekbir, Oussama A. Bensaci, Abdelkader Tifferent, Asim Abbasi, Assia Djemoui, Nazih Y. Rebouh, Abeer Hashem, Graciela Dolores Avila-Quezada, Khalid F. Almutairi and Elsayed Fathi Abd_Allah
Insects 2025, 16(8), 817; https://doi.org/10.3390/insects16080817 - 7 Aug 2025
Abstract
Broad bean is one of the most important leguminous crops worldwide. However, its productivity is greatly affected by the infestation of Aphis fabae and Aphis craccivora (Hemiptera: Aphididae). The main objective of the current study was to identify the most susceptible phenological stages [...] Read more.
Broad bean is one of the most important leguminous crops worldwide. However, its productivity is greatly affected by the infestation of Aphis fabae and Aphis craccivora (Hemiptera: Aphididae). The main objective of the current study was to identify the most susceptible phenological stages of the broad bean variety (Histal) against black aphids’ herbivory. This had been achieved through an evaluation of plant resistance mechanisms such as antixenosis and antibiosis. The results regarding an antixenosis test revealed that the four tested phenological stages of V. faba did not have a significant effect on the preference of A. craccivora and A. fabae towards the crop plant. Overall, a slightly higher number of adults settled on the three and four unfolded leaves’ stage of the crop plant. Similarly, the highest number of developed embryos were found in the four leaves’ stage of the crop, and the lowest in the second leaf stage. The adult body size of A. craccivora was slightly larger in the case of the three unfolded leaves. Furthermore, the maximum body size of A. fabae adults was recorded in the case of the first unfolded leaf stage crop. Linear correlations between the biological parameters for both species revealed only one significant relationship between developed and total embryos for A. craccivora. The results of the current study highlight the need to protect broad bean crops against infestations of black aphids, i.e., A. craccivora and A. fabae. This is essential for reducing direct damage and preventing the transmission of phytoviruses. However, future studies should aim to evaluate the susceptibility of all developmental phenological stages of the crop against black aphids to mitigate potential crop losses. Full article
(This article belongs to the Section Insect Behavior and Pathology)
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14 pages, 280 KiB  
Article
Effects of Dietary Puffed Jujube Powder on Growth Performance, Apparent Digestibility, and Meat Quality of Hainan Black Goats
by Yi Zhang, Jianzhi Shi, Jiapeng Wang, Keke Li, Xianzheng Qiao, Dong Chen, Tingting Dong, Yuanxiao Li, Yushu Zhang and Renlong Lv
Animals 2025, 15(15), 2306; https://doi.org/10.3390/ani15152306 - 6 Aug 2025
Abstract
This study was conducted to investigate the effects of puffed jujube powder (PJP) supplementation in the diet on the slaughter characteristics, growth performance, meat quality, and serum antioxidant capacity of Hainan Black (HB) goats. Twenty-four healthy male HB goats, three months old with [...] Read more.
This study was conducted to investigate the effects of puffed jujube powder (PJP) supplementation in the diet on the slaughter characteristics, growth performance, meat quality, and serum antioxidant capacity of Hainan Black (HB) goats. Twenty-four healthy male HB goats, three months old with an initial body weight of 15.12 ± 3.67 kg, were randomly divided into three groups: the 10% PJP group (basal diet plus 10% PJP); the 20% PJP group (basal diet plus 20% PJP); and the control group (basal diet only). After a 10-day adaptation period, a feeding trial was carried out for 90 days in an ad libitum diet environment. The results show that the final body weight of the 20% PJP group was markedly higher (p < 0.05) than that of the control group (22.58 ± 0.94 kg vs. 20.45 ± 1.01 kg). The average daily gain of the 20% PJP group was 83.44 ± 1.78 g/d, which was substantially greater (p < 0.05) than the 59.22 ± 2.13 g/d of the control group. The feed intake of the 20% PJP group was 713.10 ± 4.54 g/d, notably higher (p < 0.05) than the 498.20 ± 4.33 g/d of the control group. In terms of slaughter characteristics, the carcass weight of the 20% PJP group was 13.99 ± 1.22 kg, considerably heavier (p < 0.05) than the 11.79 ± 1.38 kg of the control group. The muscle weight of the 20% PJP group was 11.43 ± 1.42 kg, distinctly greater (p < 0.05) than the 9.59 ± 1.99 kg of the control group. The slaughter rate of the 20% PJP group was 42.41%, showing a notable increase (p < 0.05) compared with the 37.42% of the control group, and the net meat rate of the 20% PJP group was 34.65%, with a significant rise (p < 0.05) compared with the 30.43% of the control group. Regarding serum antioxidant capacity and meat quality, the activities of serum antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT), were conspicuously increased (p < 0.05) in the 20% PJP group. The meat shear force of the 20% PJP group was decreased by 12.9%, and the cooking loss was improved by 8.9% in comparison with the control group. In conclusion, the supplementation of 20% PJP in the diet was demonstrated to enhance the growth performance, improve the meat quality, and boost the antioxidant status of HB goats, thus presenting a feasible strategy for optimizing tropical goat production systems. Full article
(This article belongs to the Section Animal Nutrition)
18 pages, 2889 KiB  
Article
Neuroprotective Effect Against Ischemic Stroke of the Novel Functional Drink Containing Anthocyanin and Dietary Fiber Enriched-Functional Ingredient from the Mixture of Banana and Germinated Jasmine Rice
by Mubarak Muhammad, Jintanaporn Wattanathorn, Wipawee Thukham-mee, Sophida Phuthong and Supaporn Muchimapura
Life 2025, 15(8), 1222; https://doi.org/10.3390/life15081222 - 2 Aug 2025
Viewed by 154
Abstract
Due to the stroke-protective effects of dietary fiber and anthocyanin together with the synergistic interaction, we hypothesized that the functional drink containing the anthocyanins and dietary fiber-enriched functional ingredient from banana and germinated black Jasmine rice (BR) should protect against ischemic stroke. BR [...] Read more.
Due to the stroke-protective effects of dietary fiber and anthocyanin together with the synergistic interaction, we hypothesized that the functional drink containing the anthocyanins and dietary fiber-enriched functional ingredient from banana and germinated black Jasmine rice (BR) should protect against ischemic stroke. BR at doses of 300, 600, and 900 mg/kg body weight (BW) was orally given to male Wistar rats weighing 290–350 g once daily for 21 days, and they were subjected to ischemic reperfusion injury induced by temporary occlusion of the middle cerebral artery (MCAO/IR) for 90 min. The treatment was prolonged for 21 days after MCAO/IR. They were assessed for brain infarction volume, neuron density, Nrf2, MDA, and catalase in the cortex together with serum TNF-α and IL-6. Lactobacillus and Bifidobacterium spp. in feces were also assessed. Our results showed that BR improved the increase in brain infarcted volume, MDA, TNF-α, and IL-6 and the decrease in neuron density, Nrf2, catalase, and both bacteria spp. induced by MCAO/IR. These data suggest the stroke-protective effect of the novel functional drink, and the action may involve the improvement of Nrf2, oxidative stress, inflammation, and the amount of Lactobacillus and Bifidobacterium spp. Full article
(This article belongs to the Special Issue Bioactive Compounds for Medicine and Health)
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36 pages, 4836 KiB  
Article
Harnessing the Hepatoprotective and Nephroprotective Potential of Nigella sativa Fractions via per os Administration in CCl4-Intoxicated Wistar Rats: A Mixed Approach
by Mohammed Dalli, Nour Elhouda Daoudi, Salah-eddine Azizi, Mohammed Roubi, Ilyass Alami Merrouni, Faiza Souna, Mohammed Choukri, Bonglee Kim and Nadia Gseyra
Pharmaceuticals 2025, 18(8), 1147; https://doi.org/10.3390/ph18081147 - 1 Aug 2025
Viewed by 137
Abstract
Background: Nigella sativa, known as black cumin, is traditionally used to treat various illnesses. Objective: The current study aims to investigate the potential hepatoprotective and nephroprotective effect of black cumin fractions via per os route in CCl4-intoxicated Wistar rats. [...] Read more.
Background: Nigella sativa, known as black cumin, is traditionally used to treat various illnesses. Objective: The current study aims to investigate the potential hepatoprotective and nephroprotective effect of black cumin fractions via per os route in CCl4-intoxicated Wistar rats. This study used a computational approach to assess the interaction of bioactive compounds with key proteins (CYP P450 3E1, TNF-α, and Cox-2). Methods:Wistar rats were treated with CCl4 to induce liver injury and with different Nigella sativa fractions (250 mg/Kg) or Sylimarin (50 mg/Kg). Liver and kidney functions were assessed through biochemical markers, hepatic glycogen, malondialdehyde levels, molecular docking, and ADMET analysis to evaluate drug-likeliness. Results: The results revealed that intoxication with CCl4 induced an elevation in different liver and kidney biochemical parameters such as (ALT, AST, creatinine, urea...) indicating kidney and hepatic toxicity. However, treatment with different Nigella sativa fractions showed a significant improvement in animal body weight and significant amelioration of biochemical markers indicating a protective potential of these fractions against CCl4-induced intoxication. Furthermore, the molecular docking approach demonstrated high binding affinity with the target proteins. Conclusions: These current findings shed light on the therapeutic potential of Nigella sativa fractions as a promising protective agent of the liver and kidney against CCl4 intoxication. Full article
(This article belongs to the Section Natural Products)
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13 pages, 274 KiB  
Article
Nautical Desires: Tourists, Stowaways and Other Travellers in Caribbean Fiction
by Conrad Michael James
Humanities 2025, 14(8), 158; https://doi.org/10.3390/h14080158 - 31 Jul 2025
Viewed by 279
Abstract
This article examines two Caribbean texts which use 20th-century journeys on passenger ships as opportunities to investigate ways in which colonial anxieties of race and gender are worked out through nautical desires. Mayra Montero’s erotic novel La última noche que pasé contigo (1991) [...] Read more.
This article examines two Caribbean texts which use 20th-century journeys on passenger ships as opportunities to investigate ways in which colonial anxieties of race and gender are worked out through nautical desires. Mayra Montero’s erotic novel La última noche que pasé contigo (1991) and Claude McKay’s Romance in Marseille (2020) both wrestle with the imagined and material consequences of pervasive anti-blackness. They also raise crucial questions about embodied practices of struggle for survival. My analysis seeks to answer the following questions. What happens when anti-blackness masquerades as desire? How do we read and represent an anti-blackness that seeks to consume parts of the Caribbean and then dispense as refuse with what it sees as superfluous? What reading practices might we adopt in order to make sense of Caribbean bodies dehumanized on their own shores, and what narrative solutions might Caribbean fiction propose that might begin to restore humanity and value to these bodies? Full article
(This article belongs to the Special Issue Rise of a New World: Postcolonialism and Caribbean Literature)
21 pages, 8624 KiB  
Article
Comparison of GOES16 Data with the TRACER-ESCAPE Field Campaign Dataset for Convection Characterization: A Selection of Case Studies and Lessons Learnt
by Aida Galfione, Alessandro Battaglia, Mariko Oue, Elsa Cattani and Pavlos Kollias
Remote Sens. 2025, 17(15), 2621; https://doi.org/10.3390/rs17152621 - 28 Jul 2025
Viewed by 263
Abstract
Convective updrafts are one of the main characteristics of convective clouds, responsible for the convective mass flux and the redistribution of energy and condensate in the atmosphere. During the early stages of their lifecycle, convective clouds experience rapid cloud-top ascent manifested by a [...] Read more.
Convective updrafts are one of the main characteristics of convective clouds, responsible for the convective mass flux and the redistribution of energy and condensate in the atmosphere. During the early stages of their lifecycle, convective clouds experience rapid cloud-top ascent manifested by a decrease in the geostationary IR brightness temperature (TBIR). Under the assumption that the convective cloud top behaves like a black body, the ascent rate of the convective cloud top can be estimated as (TBIRt), and it can be used to infer the near cloud-top convective updraft. The temporal resolution of the geostationary IR measurements and non-uniform beam-filling effects can influence the convective updraft estimation. However, the main shortcoming until today was the lack of independent verification of the strength of the convective updraft. Here, Doppler radar observations from the ESCAPE and TRACER field experiments provide independent estimates of the convective updraft velocity at higher spatiotemporal resolution throughout the convective core column and can be used to evaluate the updraft velocity estimates from the IR cooling rate for limited samples. Isolated convective cells were tracked with dedicated radar (RHIs and PPIs) scans throughout their lifecycle. Radial Doppler velocity measurements near the convective cloud top are used to provide estimates of convective updrafts. These data are compared with the geostationary IR and VIS channels (from the GOES satellite) to characterize the convection evolution and lifecycle based on cloud-top cooling rates. Full article
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36 pages, 3579 KiB  
Article
RNA Sequencing Reveals Inflammatory and Metabolic Changes in the Lung and Brain After Carbon Black and Naphthalene Whole Body Inhalation Exposure in a Rodent Model of Military Burn Pit Exposures
by Allison M. Haaning, Brian J. Sandri, Henry L. Wyneken, William T. Goldsmith, Joshua P. Nixon, Timothy R. Nurkiewicz, Chris H. Wendt, Paul Barach, Janeen H. Trembley and Tammy A. Butterick
Int. J. Mol. Sci. 2025, 26(15), 7238; https://doi.org/10.3390/ijms26157238 - 26 Jul 2025
Viewed by 562
Abstract
Military personnel deployed to Iraq and Afghanistan were exposed to emissions from open-air burn pits, where plastics, metals, and medical waste were incinerated. These exposures have been linked to deployment-related respiratory diseases (DRRD) and may also impact neurological health via the lung–brain axis. [...] Read more.
Military personnel deployed to Iraq and Afghanistan were exposed to emissions from open-air burn pits, where plastics, metals, and medical waste were incinerated. These exposures have been linked to deployment-related respiratory diseases (DRRD) and may also impact neurological health via the lung–brain axis. To investigate molecular mechanisms, adult male rats were exposed to filtered air, naphthalene (a representative volatile organic compound), or a combination of naphthalene and carbon black (surrogate for particulate matter; CBN) via whole-body inhalation (six hours/day, three consecutive days). Lung, brain, and plasma samples were collected 24 h after the final exposure. Pro-inflammatory biomarkers were assessed using multiplex electrochemiluminescence and western blot. Differentially expressed genes (DEGs) were identified by RNA sequencing, and elastic net modeling was used to define exposure-predictive gene signatures. CBN exposure altered inflammatory biomarkers across tissues, with activation of nuclear factor kappa B (NF-κB) signaling. In the lung, gene set enrichment revealed activated pathways related to proliferation and inflammation, while epithelial–mesenchymal transition (EMT) and oxidative phosphorylation were suppressed. In the brain, EMT, inflammation, and senescence pathways were activated, while ribosomal function and oxidative metabolism were downregulated. Elastic net modeling identified a lung gene signature predictive of CBN exposure, including Kcnq3, Tgfbr1, and Tm4sf19. These findings demonstrate that inhalation of a surrogate burn pit mixture induces inflammatory and metabolic gene expression changes in both lung and brain tissues, supporting the utility of this animal model for understanding systemic effects of airborne military toxicants and for identifying potential biomarkers relevant to DRRD and Veteran health. Full article
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
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12 pages, 480 KiB  
Brief Report
Treated but Uncontrolled: Characterizing Hypertension in a Sample of 357 Older Adults in the Southeastern United States
by Rachel Helms, Laura A. Robinson, Paul S. Fiore, Kelly P. Strickland, Sarah O. Watts, Felicia J. Tuggle, Jennifer L. Slay, Jeanna Sewell and Andrew D. Frugé
Geriatrics 2025, 10(4), 101; https://doi.org/10.3390/geriatrics10040101 - 26 Jul 2025
Viewed by 174
Abstract
Background/Objectives: Hypertension (HTN) continues to be a leading cause of death and disability in older adults, especially in the southeastern United States. A cross-sectional study was conducted to evaluate the relationships among measured, diagnosed, and treated (HTN) in community-dwelling adults participating in [...] Read more.
Background/Objectives: Hypertension (HTN) continues to be a leading cause of death and disability in older adults, especially in the southeastern United States. A cross-sectional study was conducted to evaluate the relationships among measured, diagnosed, and treated (HTN) in community-dwelling adults participating in student-led health screenings in eastern Alabama. Methods: Between 2017 and 2019, students from health-related disciplines facilitated screenings at 23 community and independent living sites to conduct health assessments, including measuring blood pressure (BP), obtaining medical history, and evaluating current prescriptions. Statistical analyses including chi-square tests, t-tests, and backward stepwise linear regression were performed. Results: The current sample includes data from 357 adults aged 60 to 99 years (mean age 74.6 ± 8.7), who were 70.9% females, 60.8% identifying as Black/African American (BA), and 36.8% residing in rural areas. The majority of clients had a prior HTN diagnosis (71.1%) and/or currently measured HTN (78.7%). Forty-three percent of adults screened had measured, diagnosed, and pharmaceutically treated HTN, while 31% had measured but untreated HTN. Black clients had higher measured systolic and diastolic BP and were more likely to also have been diagnosed with HTN (p < 0.05 for all). Linear regression indicated that lower systolic BP was predicted by not living alone (p = 0.003), White race (p = 0.004), and previous HTN diagnosis (p = 0.012), while female gender (p = 0.079) and decreasing body mass index (p = 0.053) had marginal predictive value. Conclusions: These results indicate that awareness and screening of HTN in this population are noteworthy, though management of the disease through ongoing screening and referrals is essential to reduce disparities. Full article
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22 pages, 633 KiB  
Article
Effects of Genetic Diversity on Health Status and Parasitological Traits in a Wild Fish Population Inhabiting a Coastal Lagoon
by Alejandra Cruz, Esther Lantero, Carla Llinares, Laura Ortega-Díaz, Gema Castillo-García, Mar Torralva, Francisco J. Oliva-Paterna, David H. Fletcher and David Almeida
Animals 2025, 15(15), 2195; https://doi.org/10.3390/ani15152195 - 25 Jul 2025
Viewed by 176
Abstract
Host genetic variability is relevant to understanding how parasites modulate natural selection in wild fish populations. Coastal lagoons are transitional ecosystems where knowledge lacks on relationships between genotypic diversity with parasitism. The aim of this study was to assess the effect of genetic [...] Read more.
Host genetic variability is relevant to understanding how parasites modulate natural selection in wild fish populations. Coastal lagoons are transitional ecosystems where knowledge lacks on relationships between genotypic diversity with parasitism. The aim of this study was to assess the effect of genetic diversity on host health and parasitological traits in fish inhabiting a Mediterranean lagoon. Black-striped pipefish Syngnathus abaster were collected in August 2023 and 2024 from the Mar Menor (Iberian lagoon, SE Spain). Genetic diversity was measured as Internal Relatedness (IR: a homozygosity index from microsatellite markers). Population frequency was lower for the medium IR level. For this same category, both health indices (external body condition and internal organs) indicated a worse status. Parasite prevalence, abundance and an index of life-cycle complexity (heteroxenous species) were greater for the medium level of genetic diversity. Such results are explained under a scenario of parasite-mediated disruptive selection: a higher disease pressure against the phenotypically intermediate individuals. Two contrasting strategies were detected to better control parasitism at the host genotypic level: (1) high homozygosity, and (2) high heterozygosity, which probably reflects better immuno-competence as a phenotypic trait. From an evolutionary perspective, parasites play a crucial role in shaping genetic diversity within host populations. Full article
(This article belongs to the Section Animal Genetics and Genomics)
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10 pages, 439 KiB  
Article
Comparison of Angiotensin II (Giapreza®) Use in Kidney Transplantation Between Black and Non-Black Patients
by Michelle Tsai, Jamie Benken, Joshua Adisumarta, Eleanor Anderson, Chris Cheng, Adriana Ortiz, Enrico Benedetti, Hokuto Nishioka and Scott Benken
Biomedicines 2025, 13(8), 1819; https://doi.org/10.3390/biomedicines13081819 - 24 Jul 2025
Viewed by 378
Abstract
Background/Objectives: Perioperative hypotension during kidney transplantation poses a risk to graft function and survival. Angiotensin II (AngII) is an endogenous vasoconstrictor targeting the renin–angiotensin–aldosterone system (RAAS) to increase blood pressure. Black patients may have a different response to synthetic angiotensin II (AT2S) [...] Read more.
Background/Objectives: Perioperative hypotension during kidney transplantation poses a risk to graft function and survival. Angiotensin II (AngII) is an endogenous vasoconstrictor targeting the renin–angiotensin–aldosterone system (RAAS) to increase blood pressure. Black patients may have a different response to synthetic angiotensin II (AT2S) compared to non-Black patients, given differential expressions in renin profiles. The purpose of this study is to assess the difference between Black and non-Black patients in total vasopressor duration and usage when AT2S is first line for hypotension during kidney transplantation. Methods: A single-center, retrospective cohort study comparing Black and non-Black patients who required AT2S as a first-line vasopressor for hypotension during the perioperative period of kidney transplantation. Results: The primary outcome evaluating total usage of vasopressors found that Black patients required longer durations of vasopressors (36.9 ± 66.8 h vs. 23.7 ± 31.7 h; p = 0.022) but no difference in vasopressor amount (0.07 ± 0.1 NEE vs. 0.05 ± 0.1 NEE; p = 0.128) compared to non-Black patients. Regression analysis found that body weight was associated with the duration of vasopressors (p < 0.05), while baseline systolic blood pressure was inversely associated with it. Longer duration of vasopressors and duration of transplant surgery were associated with delayed graft function in regression analysis (p < 0.05). Conclusions: Black patients had a longer duration of vasopressors, but this was not driven by differences in usage of AT2S. As baseline weight was significantly higher in Black patients and associated with duration of usage, perhaps the metabolic differences in our Black patients led to the observed differences. Regardless, longer durations of vasopressors were associated with delayed graft function, making this an area of utmost importance for continued investigation. Full article
(This article belongs to the Section Drug Discovery, Development and Delivery)
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49 pages, 21554 KiB  
Article
A Disappearing Cultural Landscape: The Heritage of German-Style Land Use and Pug-And-Pine Architecture in Australia
by Dirk H. R. Spennemann
Land 2025, 14(8), 1517; https://doi.org/10.3390/land14081517 - 23 Jul 2025
Viewed by 282
Abstract
This paper investigates the cultural landscapes established by nineteenth-century German immigrants in South Australia and the southern Riverina of New South Wales, with particular attention to settlement patterns, architectural traditions and toponymic transformation. German immigration to Australia, though numerically modest compared to the [...] Read more.
This paper investigates the cultural landscapes established by nineteenth-century German immigrants in South Australia and the southern Riverina of New South Wales, with particular attention to settlement patterns, architectural traditions and toponymic transformation. German immigration to Australia, though numerically modest compared to the Americas, significantly shaped local communities, especially due to religious cohesion among Lutheran migrants. These settlers established distinct, enduring rural enclaves characterized by linguistic, religious and architectural continuity. The paper examines three manifestations of these cultural landscapes. A rich toponymic landscape was created by imposing on natural landscape features and newly founded settlements the names of the communities from which the German settlers originated. It discusses the erosion of German toponyms under wartime nationalist pressures, the subsequent partial reinstatement and the implications for cultural memory. The study traces the second manifestation of a cultural landscapes in the form of nucleated villages such as Hahndorf, Bethanien and Lobethal, which often followed the Hufendorf or Straßendorf layout, integrating Silesian land-use principles into the Australian context. Intensification of land use through housing subdivisions in two communities as well as agricultural intensification through broad acre farming has led to the fragmentation (town) and obliteration (rural) of the uniquely German form of land use. The final focus is the material expression of cultural identity through architecture, particularly the use of traditional Fachwerk (half-timbered) construction and adaptations such as pug-and-pine walling suited to local materials and climate. The paper examines domestic forms, including the distinctive black kitchen, and highlights how environmental and functional adaptation reshaped German building traditions in the antipodes. Despite a conservation movement and despite considerable documentation research in the late twentieth century, the paper shows that most German rural structures remain unlisted and vulnerable. Heritage neglect, rural depopulation, economic rationalization, lack of commercial relevance and local government policy have accelerated the decline of many of these vernacular buildings. The study concludes by problematizing the sustainability of conserving German Australian rural heritage in the face of regulatory, economic and demographic pressures. With its layering of intangible (toponymic), structural (buildings) and land use (cadastral) features, the examination of the cultural landscape established by nineteenth-century German immigrants adds to the body of literature on immigrant communities, settler colonialism and landscape research. Full article
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13 pages, 2193 KiB  
Article
Microbiota Anatomical Niche Partitioning of Simulium vanluni (Diptera: Simuliidae)
by Noor Izwan-Anas, Van Lun Low, Zubaidah Ya’cob, Sazaly AbuBakar and Kim-Kee Tan
Diversity 2025, 17(8), 504; https://doi.org/10.3390/d17080504 - 23 Jul 2025
Viewed by 280
Abstract
Background: Microbial communities of insects have distinct roles for their respective hosts. For the black fly (Diptera: Simuliidae), an important vector and ecological indicator, the representative microbiota from the different body regions are not known. Here, we investigated the microbial composition and diversity [...] Read more.
Background: Microbial communities of insects have distinct roles for their respective hosts. For the black fly (Diptera: Simuliidae), an important vector and ecological indicator, the representative microbiota from the different body regions are not known. Here, we investigated the microbial composition and diversity of the head, thorax, and abdomen of wild-caught Simulium vanluni. Methods: Adult Simulium vanluni were surface-sterilized and dissected into head, thorax, and abdomen. For each body region, 20 individuals were pooled into one sample with six replicates per region. DNA was extracted and sequenced using the 16S rRNA amplification method to assess for possible microbial diversity. Data were analyzed using MicrobiomeAnalyst, where we calculated alpha diversity, beta diversity, and tested compositional differences using PERMANOVA. Results: Across 17 pooled samples, three core genera, Wolbachia (78.33%), Rickettsia (9.74%), and Acinetobacter (9.20%), accounted for more than 97% of the 16S rRNA sequencing reads. Head communities were compositionally distinct compared to the thorax and abdomen (PERMANOVA, p < 0.05). Heads were nearly monodominated by Wolbachia (95–97%), exhibiting significantly lower diversity and evenness compared to other body regions. In contrast, the thoracic and abdominal communities were more even, where thoraces were enriched with Acinetobacter (19.16%) relative to Rickettsia (10.85%), while abdomens harbored higher Rickettsia (10.96%) than Acinetobacter (5.68%). Collectively, the near-monodominance of Wolbachia in heads and inverse abundances of Acinetobacter and Rickettsia in thoraces and abdomens suggest possible anatomical niche partitioning or competition exclusion of microbiota across body regions. Conclusions: Our findings reveal fine-scale anatomical niche partitioning in S. vanluni microbiota, with the heads being almost exclusively colonized by Wolbachia, while the thoracic and abdominal niche regions exhibit distinct enrichment patterns for Acinetobacter and Rickettsia. These spatially distinct microbial distributions suggest potential functional specialization across anatomical regions of S. vanluni. Full article
(This article belongs to the Special Issue Diversity, Biodiversity, Threats and Conservation of Arthropods)
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15 pages, 1273 KiB  
Article
Screening of Substrates and Optimization of Formulations for Exogenous Nutrient Bags of Morchella sextelata (Black Morel)
by Qi Yan, Weidong Zhang, Qi Wang, Tonghui Yang, Peng Wang, Ya Yu, Xiao Tan, Xueping Kang and Jiawei Wen
Horticulturae 2025, 11(7), 863; https://doi.org/10.3390/horticulturae11070863 - 21 Jul 2025
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Abstract
In the artificial cultivation of Morchella sextelata (Black Morel), exogenous nutrient bags (ENBs) commonly employ wheat grains as the primary substrate raw material. However, this approach is costly and runs counter to the “non-grain” development direction advocated by the edible mushroom industry. Under [...] Read more.
In the artificial cultivation of Morchella sextelata (Black Morel), exogenous nutrient bags (ENBs) commonly employ wheat grains as the primary substrate raw material. However, this approach is costly and runs counter to the “non-grain” development direction advocated by the edible mushroom industry. Under controlled field conditions, twelve self-made formulations were set up and compared with a conventional market formulation to comprehensively analyze their impacts on the agronomic traits, yield, soil physicochemical properties, and economic benefits of M. sextelata fruiting bodies. The research findings indicate that the nutrient bag formulations have a significant effect on soil available nutrients. Specifically, the contents of alkali-hydrolysable nitrogen (AN) and available potassium (AK) exhibit a significantly negative correlation with M. sextelata yield (r = −0.60, p < 0.05; r = −0.72, p < 0.01, respectively). Among all the treatment groups, the KY1 formulation (comprising 30% wheat grains, 5% rice bran, 60% corncobs, 2% rice husks, 1% lime, and 1% gypsum) achieved the highest yield of 915.13 kg per 667 m2, which was 16.1% higher than that of the control group. The net economic benefit per unit area (667 m2) reached CNY 75,282.15, representing a 20.7% increase compared to the traditional wheat grains-based formulation. In conclusion, partially substituting wheat grains with rice bran in ENBs can not only reduce reliance on staple food resources but also enhance yield and economic efficiency. Due to the differences in cultivated strains and environmental conditions, the impact on morel yield is substantial; therefore, the results of this study need further validation through pilot trials. Full article
(This article belongs to the Section Medicinals, Herbs, and Specialty Crops)
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12 pages, 351 KiB  
Article
Associations Between Sleep, Appetite, and Food Reward over 6 Months in Black Emerging Adults—Findings from the Sleep, Health Outcomes and Body Weight (SHOW) Pilot Study
by Hannah R. Koch, Jesse N. L. Sims, Stephanie Pickett, Graham Finlayson, Laurie Wideman and Jessica McNeil
Nutrients 2025, 17(14), 2305; https://doi.org/10.3390/nu17142305 - 13 Jul 2025
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Abstract
Background/Objectives: Imposed sleep restriction leads to increased feelings of appetite and hedonic eating behaviors (or food rewards). No study to date has assessed home-based measures of sleep with appetite and food rewards exclusively in Black emerging adults (ages 18–28 years), despite higher [...] Read more.
Background/Objectives: Imposed sleep restriction leads to increased feelings of appetite and hedonic eating behaviors (or food rewards). No study to date has assessed home-based measures of sleep with appetite and food rewards exclusively in Black emerging adults (ages 18–28 years), despite higher risks of short sleep and obesity in this population. We examined associations between 6-month changes in sleep with changes in appetite and food reward in Black emerging adults. Methods: Fifteen Black emerging adults (12 females; age, 21 ± 2.5 years; body mass index, 25.7 ± 4.5 kg/m2; body fat, 25.8 ± 11.9%) completed two identical 7-day measurement bursts at baseline and 6 months. Sleep (duration, efficiency, and architecture) was captured via 7 days of actigraphy and 2 nights of in-home polysomnography. During a laboratory visit, participants completed appetite measures (desire to eat, hunger, fullness, and prospective food consumption) via visual analog scales before and for 3 h following standard breakfast intake. The food reward for the fat and sweet categories of food was measured before lunch with the Leeds Food Preference Questionnaire. Results: Fasting fullness scores decreased from baseline to 6 months (−8.9 mm, p < 0.01) despite increases in body weight (2.6 kg, p < 0.01) and waist circumference (2.4 cm, p = 0.03). Increases in actigraph-measured sleep duration were associated with decreases in fasting desire to eat (r = −0.58, p = 0.04). Increases in actigraph-measured sleep efficiency were also associated with decreases in explicit liking for sweet foods (r = −0.60, p = 0.03). Conclusions: Our findings suggest that improvements in sleep duration and sleep efficiency may lead to decreased feelings of appetite and food reward in Black emerging adults. Full article
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