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Pharmaceuticals
Volume 18
Issue 8
10.3390/ph18081147
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Article

Harnessing the Hepatoprotective and Nephroprotective Potential of Nigella sativa Fractions via per os Administration in CCl4-Intoxicated Wistar Rats: A Mixed Approach

by
Mohammed Dalli
1,2,*,
Nour Elhouda Daoudi
1,2,
Salah-eddine Azizi
1,
Mohammed Roubi
2,
Ilyass Alami Merrouni
1,
Faiza Souna
1,
Mohammed Choukri
3,
Bonglee Kim
4,5,* and
Nadia Gseyra
2
1
Higher Institute of Nursing Professions and Health Techniques, Oujda 60000, Morocco
2
Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, University Mohammed the First, Oujda 60000, Morocco
3
Central Laboratory, Biochemistry Department of Mohammed VI University Hospital Center, Oujda 60000, Morocco
4
College of Korean Medicine, Kyung Hee University, Hoegidong Dongdaemungu, Seoul 05253, Republic of Korea
5
Korean Medicine-Based Drug Repositioning Cancer Research Center, College of Korean Medicine, Kyung Hee University, Hoegidong Dongdaemungu, Seoul 05253, Republic of Korea
*
Authors to whom correspondence should be addressed.
Pharmaceuticals 2025, 18(8), 1147; https://doi.org/10.3390/ph18081147 (registering DOI)
Submission received: 17 February 2025 / Revised: 21 March 2025 / Accepted: 30 July 2025 / Published: 1 August 2025
(This article belongs to the Section Natural Products)
Versions Notes

Abstract

Background: Nigella sativa, known as black cumin, is traditionally used to treat various illnesses. Objective: The current study aims to investigate the potential hepatoprotective and nephroprotective effect of black cumin fractions via per os route in CCl4-intoxicated Wistar rats. This study used a computational approach to assess the interaction of bioactive compounds with key proteins (CYP P450 3E1, TNF-α, and Cox-2). Methods: Wistar rats were treated with CCl4 to induce liver injury and with different Nigella sativa fractions (250 mg/Kg) or Sylimarin (50 mg/Kg). Liver and kidney functions were assessed through biochemical markers, hepatic glycogen, malondialdehyde levels, molecular docking, and ADMET analysis to evaluate drug-likeliness. Results: The results revealed that intoxication with CCl4 induced an elevation in different liver and kidney biochemical parameters such as (ALT, AST, creatinine, urea...) indicating kidney and hepatic toxicity. However, treatment with different Nigella sativa fractions showed a significant improvement in animal body weight and significant amelioration of biochemical markers indicating a protective potential of these fractions against CCl4-induced intoxication. Furthermore, the molecular docking approach demonstrated high binding affinity with the target proteins. Conclusions: These current findings shed light on the therapeutic potential of Nigella sativa fractions as a promising protective agent of the liver and kidney against CCl4 intoxication.
Keywords: Nigella sativa; liver protection; kidney protection; molecular docking; ADMET Nigella sativa; liver protection; kidney protection; molecular docking; ADMET

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MDPI and ACS Style

Dalli, M.; Daoudi, N.E.; Azizi, S.-e.; Roubi, M.; Alami Merrouni, I.; Souna, F.; Choukri, M.; Kim, B.; Gseyra, N. Harnessing the Hepatoprotective and Nephroprotective Potential of Nigella sativa Fractions via per os Administration in CCl4-Intoxicated Wistar Rats: A Mixed Approach. Pharmaceuticals 2025, 18, 1147. https://doi.org/10.3390/ph18081147

AMA Style

Dalli M, Daoudi NE, Azizi S-e, Roubi M, Alami Merrouni I, Souna F, Choukri M, Kim B, Gseyra N. Harnessing the Hepatoprotective and Nephroprotective Potential of Nigella sativa Fractions via per os Administration in CCl4-Intoxicated Wistar Rats: A Mixed Approach. Pharmaceuticals. 2025; 18(8):1147. https://doi.org/10.3390/ph18081147

Chicago/Turabian Style

Dalli, Mohammed, Nour Elhouda Daoudi, Salah-eddine Azizi, Mohammed Roubi, Ilyass Alami Merrouni, Faiza Souna, Mohammed Choukri, Bonglee Kim, and Nadia Gseyra. 2025. "Harnessing the Hepatoprotective and Nephroprotective Potential of Nigella sativa Fractions via per os Administration in CCl4-Intoxicated Wistar Rats: A Mixed Approach" Pharmaceuticals 18, no. 8: 1147. https://doi.org/10.3390/ph18081147

APA Style

Dalli, M., Daoudi, N. E., Azizi, S.-e., Roubi, M., Alami Merrouni, I., Souna, F., Choukri, M., Kim, B., & Gseyra, N. (2025). Harnessing the Hepatoprotective and Nephroprotective Potential of Nigella sativa Fractions via per os Administration in CCl4-Intoxicated Wistar Rats: A Mixed Approach. Pharmaceuticals, 18(8), 1147. https://doi.org/10.3390/ph18081147

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