Search Results (1,272)

Multivalent lectin–glycan interactions (MLGIs) are vital for viral infection, cell-cell communication and regulation of immune responses. Their structural and biophysical data are thus important, not only for providing insights into their underlying mechanisms but also for designing potent glycoconjugate therapeutics against target MLGIs. However, such information remains to be limited for some important MLGIs, significantly restricting the research progress. We have recently demonstrated that functional nanoparticles, including ∼4 nm quantum dots and varying sized gold nanoparticles (GNPs), densely glycosylated with various natural mono- and oligo- saccharides, are powerful biophysical probes for MLGIs. Using two important viral receptors, DC-SIGN and DC-SIGNR (together denoted as DC-SIGN/R hereafter), as model multimeric lectins, we have shown that α-mannose and α-manno-α-1,2-biose (abbreviated as Man and DiMan, respectively) coated GNPs not only can provide sensitive measurement of MLGI affinities but also reveal critical structural information (e.g., binding site orientation and mode) which are important for MLGI targeting. In this study, we produced mannuronic acid (ManA) coated GNPs (GNP-ManA) of two different sizes to probe the effect of glycan modification on their MLGI affinity and antiviral property. Using our recently developed GNP fluorescence quenching assay, we find that GNP-ManA binds effectively to both DC-SIGN/R and increasing the size of GNP significantly enhances their MLGI affinity. Consistent with this, increasing the GNP size also significantly enhances their ability to block DC-SIGN/R-augmented virus entry into host cells. Particularly, ManA coated 13 nm GNP potently block Ebola virus glycoprotein-driven entry into DC-SIGN/R-expressing cells with sub-nM levels of EC₅₀. Our findings suggest that GNP-ManA probes can act as a useful tool to quantify the characteristics of MLGIs, where increasing the GNP scaffold size substantially enhances their MLGI affinity and antiviral potency. Full article

The distribution of forest aboveground biomass density (AGBD) is a key indicator of carbon stock and ecosystem health in the Eastern Himalayas, which represents a global biodiversity hotspot that sustains diverse forest types across an elevation gradient from lowland rainforests to alpine meadows and contributes to the livelihoods of more than 200 distinct indigenous communities. This study aimed to identify the key factors influencing forest AGBD across this region by analyzing the underlying biophysical and anthropogenic drivers through machine learning (random forest). We processed AGBD data from the Global Ecosystem Dynamics Investigation (GEDI) spaceborne LiDAR and applied filtering to retain 30,257 high-quality footprints across ten ecoregions. We then analyzed the relationship between AGBD and 17 climatic, topographic, soil, and anthropogenic variables using random forest regression models. The results revealed significant spatial variability in AGBD (149.6 ± 79.5 Mg ha⁻¹) across the region. State-wise, Sikkim recorded the highest mean AGBD (218 Mg ha⁻¹) and Manipur the lowest (102.8 Mg ha⁻¹). Within individual ecoregions, the Himalayan subtropical pine forests exhibited the highest mean AGBD (245.5 Mg ha⁻¹). Topographic factors, particularly elevation and latitude, were strong determinants of biomass distribution, with AGBD increasing up to elevations of 2000 m before declining. Protected areas (PAs) consistently showed higher AGBD than unprotected forests for all ecoregions, while proximity to urban and agricultural areas resulted in lower AGBD, pointing towards negative anthropogenic impacts. Our full model explained 41% of AGBD variance across the Eastern Himalayas, with better performance in individual ecoregions like the Northeast India-Myanmar pine forests (R² = 0.59). While limited by the absence of regionally explicit stand-level forest structure data (age, stand density, species composition), our results provide valuable evidence for conservation policy development, including expansion of PAs, compensating avoided deforestation and modifications in shifting cultivation. Future research should integrate field measurements with remote sensing and use high-resolution LiDAR with locally derived allometric models to enhance biomass estimation and GEDI data validation. Full article

This study examines diurnal surface temperature dynamics across major Moroccan cities during the growing season and explores the interaction between urban and vegetated surfaces. We also introduce the Urban Thermal Impact Ratio (UTIR), a novel metric designed to quantify urban thermal comfort as a function of the surface urban heat island (SUHI) intensity. The analysis is based on outputs from a land surface model (LSM) for the year 2010, integrating high-resolution Landsat and MODIS data to characterize land cover and biophysical parameters across twelve land cover types. Our findings reveal moderate urban–vegetation temperature differences in coastal cities like Tangier (1.8 °C) and Rabat (1.0 °C), where winter vegetation remains active. In inland areas, urban morphology plays a more dominant role: Fes, with a 20% impervious surface area (ISA), exhibits a smaller SUHI than Meknes (5% ISA), due to higher urban heating in the latter. The Atlantic desert city of Dakhla shows a distinct pattern, with a nighttime SUHI of 2.1 °C and a daytime urban cooling of −0.7 °C, driven by irrigated parks and lawns enhancing evapotranspiration and shading. At the regional scale, summer UTIR values remain below one in Tangier-Tetouan-Al Hoceima, Rabat-Sale-Kenitra, and Casablanca-Settat, suggesting that urban conditions generally stay within thermal comfort thresholds. In contrast, higher UTIR values in Marrakech-Safi, Beni Mellal-Khénifra, and Guelmim-Oued Noun indicate elevated heat discomfort. At the city scale, the UTIR in Tangier, Rabat, and Casablanca demonstrates a clear diurnal pattern: it emerges around 11:00 a.m., peaks at 1:00 p.m., and fades by 3:00 p.m. This study highlights the critical role of vegetation in regulating urban surface temperatures and modulating urban–rural thermal contrasts. The UTIR provides a practical, scalable indicator of urban heat stress, particularly valuable in data-scarce settings. These findings carry significant implications for climate-resilient urban planning, optimized energy use, and the design of public health early warning systems in the context of climate change. Full article

Ultra-high dose rate radiotherapy known as Flash radiotherapy (FLASH-RT) offers tremendous opportunities to improve the therapeutic ratio of radiotherapy by sparing the normal tissue while maintaining similar tumoricidal efficacy. However, the underlying biophysical basis of the FLASH effect remains under active investigation with several proposed mechanisms involving oxygen depletion, altered free-radical chemistry, and differential biological responses. This article provides an overview of available experimental and computational tools that can be utilized to probe the tumor and normal tissue microenvironment. We analyze in vitro, ex vivo, and in vivo systems used to study FLASH responses. We describe various computational and imaging technologies that can potentially aid in understanding the biophysics of FLASH-RT and lead to safer clinical translational. Full article

Cassava is the sixth most important food crop and is cultivated in more than 100 countries. The crop tolerates low soil fertility and drought, enabling it to play a role in climate adaptation strategies. Cassava generally requires careful preparation to remove toxic hydrogen cyanide (HCN) before its consumption, but HCN concentrations can vary considerably between varieties. Climate change and low inputs, particularly carbon and nutrients, affect agriculture in Pacific Island countries where cassava is commonly grown alongside traditional crops (e.g., taro). Despite increasing popularity in this region, there is limited experimental data about cassava crop management for different local varieties, their relative toxicity and nutritional value for human consumption, and their interaction with changing climate conditions. To help address this knowledge gap, three field experiments were conducted at the Koronivia Research Station of the Fiji Ministry of Agriculture. Two varieties of cassava with contrasting HCN content were planted at three different times coinciding with the start of the wet (September-October) or dry (April) seasons. A time series of measurements was conducted during the full 18-month or differing 6-month durations of each crop, based on destructive harvests and phenological observations. The former included determination of total biomass, HCN potential, carbon isotopes (δ¹³C), and elemental composition. Yield and nutritional value were significantly affected by variety and time of planting, and there were interactions between the two factors. Findings from this work will improve cassava management locally and will provide a valuable dataset for agronomic and biophysical model testing. Full article

Groundwater level monitoring is crucial for assessing hydrological responses to climate change and human activities, which pose significant threats to the sustainability of semi-arid aquifers and the cultural heritage they sustain. This study presents an integrated remote sensing and transformer-based deep learning framework that combines diverse geospatial datasets to predict spatiotemporal variations across the plain near the Persepolis and Naqsh-e Rustam archaeological complexes—UNESCO World Heritage Sites situated at the plain’s edge. We assemble 432 synthetic aperture radar (SAR) scenes (2015–2022) and derive vertical ground motion rates greater than −180 mm yr⁻¹, which are co-localized with multisource geoinformation, including hydrometeorological indices, biophysical parameters, and terrain attributes, to train transformer models with traditional deep learning methods. A sparse probabilistic transformer (ConvTransformer) trained on 95 gridded variables achieves an out-of-sample R² = 0.83 and RMSE = 6.15 m, outperforming bidirectional deep learning models by >40%. Scenario analysis indicates that, in the absence of intervention, subsidence may exceed 200 mm per year within a decade, threatening irreplaceable Achaemenid stone reliefs. Our results indicate that attention-based networks, when coupled to synergistic geodetic constraints, enable early-warning quantification of groundwater stress over heritage sites and provide a scalable template for sustainable aquifer governance worldwide. Full article

Accurate crop yield prediction is essential for stabilizing food supply chains and reducing the uncertainties in financial risks related to agricultural production. Yet, it is even more essential to understand how crop yield models make predictions depending on their relationship to Earth Observation (EO) indicators. This study presents a state-of-the-art explainable artificial intelligence (XAI) method to estimate corn yield prediction over the Corn Belt in the continental United States (CONUS). We utilize the recently introduced Kolmogorov–Arnold Network (KAN) architecture, which offers an interpretable alternative to the traditional Multi-Layer Perceptron (MLP) approach by utilizing learnable spline-based activation functions instead of fixed ones. By including a KAN in our crop yield prediction framework, we are able to achieve high prediction accuracy and identify the temporal drivers behind crop yield variability. We create a multi-source dataset that includes biophysical parameters along the crop phenology, as well as meteorological, topographic, and soil parameters to perform end-of-season and in-season predictions of county-level corn yields between 2016–2023. The performance of the KAN model is compared with the commonly used traditional machine learning (ML) models and its architecture-wise equivalent MLP. The KAN-based crop yield model outperforms the other models, achieving an R² of 0.85, an RMSE of 0.84 t/ha, and an MAE of 0.62 t/ha (compared to MLP: R² = 0.81, RMSE = 0.95 t/ha, and MAE = 0.71 t/ha). In addition to end-of-season predictions, the KAN model also proves effective for in-season yield forecasting. Notably, even three months prior to harvest, the KAN model demonstrates strong performance in in-season yield forecasting, achieving an R² of 0.82, an MAE of 0.74 t/ha, and an RMSE of 0.98 t/ha. These results indicate that the model maintains a high level of explanatory power relative to its final performance. Overall, these findings highlight the potential of the KAN model as a reliable tool for early yield estimation, offering valuable insights for agricultural planning and decision-making. Full article

Forests provide habitat for diverse species and play a key role in mitigating climate change. Remote sensing enables efficient monitoring of many forest attributes across vast areas, thus supporting effective and efficient management strategies. This study aimed to identify an effective combination of remote sensing sensors for estimating biophysical variables in Mediterranean and temperate forests that can be easily translated into an operational context. Aboveground biomass (AGB), canopy height (CH), and forest canopy cover (FCC) were estimated using a combination of optical (Sentinel-2, Landsat) and radar sensors (Sentinel-1 and TerraSAR-X/TanDEM-X), along with records of past forest disturbances and topography-related variables. As a reference, lidar-derived AGB, CH, and FCC were used. Model performance was assessed not only with standard approaches such as out-of-bag sampling but also with completely independent lidar-derived reference datasets, thus enabling evaluation of the model’s temporal inference capacity. In Mediterranean forests, models based on optical imagery outperformed the radar-enhanced models when estimating FCC and CH, with elevation and spectral indices being key predictors of forest structure. In contrast, in denser temperate forests, radar data (especially X-band relative heights) were crucial for estimating CH and AGB. Incorporating past disturbance data further improved model accuracy in these denser ecosystems. Overall, this study underscores the value of integrating multi-source remote sensing data while highlighting the limitations of temporal extrapolation. The presented methodology can be adapted to enhance forest variable estimation across many forest ecosystems. Full article

Cell membrane capacitance (C_m) is a potential biomarker that reflects the structural and functional integrity of cell membranes. It is essential for physiological processes such as signal transduction, ion transport, and cellular homeostasis. In clinical practice, C_m can be determined using bioimpedance spectroscopy (BIS), a non-invasive technique for analysing the intrinsic electrical properties of biological tissues across a range of frequencies. C_m may be relevant in various clinical fields, where high capacitance is associated with healthy and intact membranes, while low capacitance indicates cellular damage or disease. Despite its promise as a prognostic indicator, several knowledge gaps limit the broader clinical application of C_m. These include variability in measurement techniques (e.g., electrode placement, frequency selection), the lack of standardised measurement protocols, uncertainty on how C_m is related to pathology, and the relatively low amount of C_m research. By addressing these gaps, C_m may become a valuable tool for examining cellular health, early disease detection, and evaluating treatment efficacy in clinical practice. This review explores the fundamental principles of C_m measured with the BIS technique, its mathematical basis and relationship to the biophysical Cole model, and its potential clinical applications. It identifies current gaps in our knowledge and outlines future research directions to enhance the understanding and use of C_m. For example, C_m has shown promise in identifying membrane degradation in sepsis, predicting malnutrition in anorexia nervosa, and as a prognostic factor in cancer. Full article

Machine learning (ML) has gained significant attention for unraveling the complex, nonlinear relationships between soil moisture (SM) and various predictive variables, including remote sensing (RS; reflectance, brightness temperature, backscatter coefficients) and biophysical (topographic, soil, vegetation, and weather) variables. We reviewed the literature to extract and synthesize ML algorithms, reliable input features, and challenges in SM estimation using RS data. We analyzed results from 144 articles published from 2010 to 2024. Random forest (40 out of 67 studies), support vector regressor (13 out of 39 studies), and artificial neural networks (12 out of 27 studies) often outperformed other algorithms to estimate SM using RS datasets. Multi-source RS data often outperformed single-source data in SM estimation. Satellite-derived features, such as vegetation indices and backscattering coefficients, provided critical information on surface SM (SSM) variability to estimate SSM. For root zone SM estimation, soil properties and SSM generally were more reliable predictors than surface information derived solely from RS. Two recent advances—the use of semi-empirical models and L-band SAR to mitigate vegetation effects, and transfer learning to improve model transferability—have shown promise in addressing key challenges in SM estimation. Full article

Despite significant advances in understanding neuronal development, a fully quantitative framework that integrates intracellular mechanisms with environmental cues during axonal growth remains incomplete. Here, we present a unified biophysical model that captures key mechanochemical processes governing axonal extension on micropatterned substrates. In these environments, axons preferentially align with the pattern direction, form bundles, and advance at constant speed. The model integrates four core components: (i) actin–adhesion traction coupling, (ii) lateral inhibition between neighboring axons, (iii) tubulin transport from soma to growth cone, and (iv) orientation dynamics guided by substrate anisotropy. Dynamical systems analysis reveals that a saddle–node bifurcation in the actin adhesion subsystem drives a transition to a high-traction motile state, while traction feedback shifts a pitchfork bifurcation in the signaling loop, promoting symmetry breaking and robust alignment. An exact linear solution in the tubulin transport subsystem functions as a built-in speed regulator, ensuring stable elongation rates. Simulations using experimentally inferred parameters accurately reproduce elongation speed, alignment variance, and bundle spacing. The model provides explicit design rules for enhancing axonal alignment through modulation of substrate stiffness and adhesion dynamics. By identifying key control parameters, this work enables rational design of biomaterials for neural repair and engineered tissue systems. Full article

Multispectral images obtained from Unmanned Aerial Vehicles (UAVs) have become strategic tools in precision agriculture, particularly for analyzing spatial variability in soil attributes. This study aimed to evaluate the spatial distribution of soil electrical (EC) and total organic carbon (TOC) in irrigated forage cactus areas in the Brazilian semiarid region, using field measurements and UAV-based multispectral imagery. The study was conducted in a communal agricultural settlement located in the Mimoso Alluvial Valley (MAV), where EC and TOC were measured at 96 points, and seven biophysical indices were derived from UAV multispectral imagery. Geostatistical models, including cokriging with spectral indices (NDVI, EVI, GDVI, SAVI, and NDSI), were applied to map soil attributes at different spatial scales. Cokriging improved the spatial prediction of EC and TOC by reducing uncertainty and increasing mapping accuracy. The standard deviation of EC decreased from 1.39 (kriging) to 0.67 (cokriging with EVI), and for TOC from 15.55 to 8.78 (cokriging with NDVI and NDSI), reflecting a 43.5% reduction in uncertainty. The indices, EVI, NDVI, and NDSI, showed strong potential in representing and enhancing the spatial variability in soil attributes. NDVI and NDSI were particularly effective at finer grid resolutions, supporting more efficient irrigation strategies and sustainable agricultural practices. Full article

Climate change (CC) is a major threat to agriculture, the sector that supports the territorial economy in the Pays Haut Languedoc et Vignoble (PHLV) region (south France). In this region, farms have been facing the negative effects of CC for several decades. The implementation of agriculture adaptation policies requires a coherent and integrated tool that mobilizes approaches for territorial development, vulnerability assessments, and feasibility. The purpose of this research is to provide a multi-criteria assessment of farm vulnerability to CC in the PHLV region. An index of farm vulnerability was developed based on the classic model of vulnerability, which is the product of exposure and sensitivity divided by adaptive capacity. This assessment was conducted at the farm level, by combining biophysical variables (such as soil type and irrigation) and socioeconomic variables (such as agricultural experience and crop insurance), selected based on a literature review and interviews with local stakeholders and local experts. To solve the weighting problem, we differentiated between a “calculated vulnerability”, without any specific weighting of the vulnerability variables, and a “declared vulnerability” that integrates the scores assigned to the importance of each variable for each farmer surveyed, based on their awareness. Afterward, a discriminant analysis was used to identify the factors that determine the vulnerability classes. Our results show that (i) the majority of the surveyed farms have a relatively high vulnerability index, but wine farms and cereal farms are the most vulnerable; (ii) for all farms the “declared vulnerability” is lower than the “calculated vulnerability”, showing that farmers underestimate their vulnerability; (iii) there is an interesting link between the low level of vulnerability and the adaptation efforts already made over the past ten years by certain farms that have changed or introduced crops and improved their agricultural practices. Full article

In this study, we introduce Shannon entropy as a key metric for assessing concentration variability in diffusion processes. Shannon entropy quantifies the uncertainty or disorder in the spatial distribution of diffusing particles, providing a novel perspective on diffusion dynamics. This proposed approach enables a more comprehensive characterization of mixing efficiency, equilibrium states, and transient diffusion behavior. Numerical simulations performed using the finite difference method in the MAPLE 2025 symbolic computing environment illustrate how entropy evolution correlates with diffusion kinetics. The computational model used in this study is based on a previously developed framework from our earlier research, ensuring consistency and validation of the results. The findings suggest that Shannon entropy can serve as a robust descriptor of diffusion-driven mixing, with potential applications in engineering, environmental science, and biophysics. Full article

Canopy chlorophyll content (CCC) is a key indicator for assessing the carbon sequestration capacity and material cycling efficiency of ecosystems, and its accurate retrieval holds significant importance for analyzing ecosystem functioning. Although numerous destructive and remote sensing methods have been developed to estimate CCC, the accurate estimation of CCC remains a significant challenge in mountainous regions with complex terrain and heterogeneous vegetation types. Through the synergistic analysis of ground hyperspectral and Sentinel-2 data, this study employed Pearson correlation analysis and spectral resampling techniques to identify Sentinel-2 blue band B1 (443 nm) and red band B4 (665 nm) as chlorophyll-sensitive bands through spectral matching with the hyperspectral reflectance of typical grassland vegetation. Based on this, we developed a new four-band vegetation index (VI), the Dual Red-edge and Coastal Aerosol Vegetation Index (DRECAVI), for estimating the CCC of heterogeneous grasslands in the middle section of the Tianshan Mountains. DRECAVI incorporates red-edge anti-saturation modules (bands B4 and B7) and aerosol correction modules (bands B1 and B8). In order to test the performance of the new index, we compared it with eight commonly used indices and a hybrid model, the Sentinel-2 Biophysical Processor (S2BP). The results indicated the following: (1) DRECAVI demonstrated the highest accuracy in CCC retrieval for mountainous vegetation (R² = 0.74, RMSE = 16.79, MAE = 12.50) compared to other VIs and hybrid methods, effectively mitigating saturation effects in high biomass areas and capturing a weak bimodal distribution pattern of CCC in the montane meadow. (2) The blue band B1 enhances atmospheric correction robustness by suppressing aerosol scattering, and the red-edge band B7 overcomes the sensitivity limitations of conventional red-edge indices (such as NDVI705, CIred-edge, and NDRE), demonstrating the potential application of the synergy mechanism between the blue band and the red-edge band. (3) Although the S2BP achieved high accuracy (R² = 0.73, RMSE = 19.83, MAE = 14.71) without saturation effects and detected a bimodal distribution of CCC in the montane meadow of the study area, its algorithmic complexity hindered large-scale operational applications. In contrast, DRECAVI maintained similar precision while reducing algorithmic complexity, making it more suitable for regional-scale grassland dynamic monitoring. This study confirms that the synergistic use of multi-source data effectively overcomes the limitations of the spectral–spatial resolution of a single data source, providing a novel methodology for the precision monitoring of mountain ecosystems. Full article