Search Results (10,328)

The biological compatibility of endodontic sealers is a key determinant of periapical tissue healing. This in vitro study investigated the cytotoxic, pro-inflammatory, and redox-related effects of eight endodontic sealers on human periodontal ligament fibroblasts (HPdLFs): Biopulp (Chema-Elektromet), AH Plus (Dentsply Sirona), MTA Fillapex (Angelus), EndoSeal MTA (Maruchi), GuttaFlow (Coltène), AH Plus Bioceramic (Dentsply Sirona), TotalFill BC (FKG Dentaire SA), and BioRoot TM (Septodont). Cells were exposed for 24 h to 10-fold-diluted sealer extracts prepared in accordance with the manufacturers’ instructions, while control samples underwent identical procedures without sealer contact. Oxidative stress biomarkers, antioxidant defense parameters, protein oxidation indices, apoptotic activity (caspase-3), pro-inflammatory cytokines (IL-1, IL-6), and cell viability (MTT assay) were assessed. Under the applied conditions, all materials induced only limited global oxidative stress, with most alterations reflecting selective protein and glycoxidative modifications. Nevertheless, AH Plus, MTA Fillapex, and the calcium hydroxide-based Biopulp exhibited a less favorable redox profile and greater protein oxidation compared with calcium silicate-based sealers. AH Plus and EndoSeal MTA were associated with increased IL-6 release, whereas EndoSeal MTA moderately elevated IL-1 levels. BioRoot TM demonstrated the lowest cytokine expression, and TotalFill BC preserved high cell viability. Caspase-3 activity remained comparable across all experimental groups, indicating minimal induction of apoptosis. Full article

Filamentous fungi are increasingly recognized as versatile biological platforms for the development of advanced (bio)sensing technologies, owing to their extensive secretory capacity, material-forming ability, and intrinsic bioelectrical activity. This review critically surveys recent progress in fungal-based sensing within a multiscale framework spanning molecular, material, computational, and ecological domains, with particular emphasis on developments reported over the past five years. Key advances involving secretome-derived biomolecules, mycogenic nanomaterials, mycelium-based living materials, and fungal electrophysiology are discussed alongside emerging approaches for environmental monitoring that integrate sensor networks, imaging platforms, and data-driven analytics. Collectively, these works demonstrate that fungal systems can enhance biosensor sensitivity, selectivity, and sustainability, while enabling unconventional paradigms of signal transduction, material-integrated sensing, and biologically mediated computation. At larger spatial and temporal scales, mycelial growth dynamics and electrical activity provide measurable responses to mechanical, chemical, and environmental perturbations, supporting early applications in wearable devices, structural materials, and ecosystem monitoring. Despite significant progress, challenges remain in reproducibility, long-term stability, mechanistic understanding, and scalable device integration. Overall, the evidence reviewed highlights filamentous fungi as biologically adaptive and ecologically embedded systems with substantial potential to support next-generation (bio)sensing technologies, while underscoring the need for integrative approaches that combine biological insight with materials science, electronics, and artificial intelligence. Full article

The widespread use of conventional plastic mulch films in agriculture contributes significantly to soil pollution due to their non-biodegradable nature. This study explores the potential of novel bio-based mulch films composed of chitosan, carboxymethyl cellulose, and sodium alginate, formulated in different ratios (1:1 and 17:3), with or without enrichment with monoammonium phosphate (MAP), to serve as biodegradable films with potential nutrient-releasing functionality as alternatives to conventional plastics. A multi-analytical approach, including elemental and isotopic analysis (EA-IRMS), biodegradation assays, and pyrolysis–gas chromatography–mass spectrometry (Py-GC-MS), was employed to assess their chemical properties, degradation behavior, and environmental compatibility. The results demonstrated that the 1:1 films, both with and without MAP, achieved over 90% biodegradation within 120 days under controlled soil conditions, in agreement with international criteria for soil biodegradability. In contrast, the 17:3 films showed reduced degradation, especially without MAP enrichment, highlighting the influence of polymer composition on microbial degradation. Isotopic tracing confirmed MAP integration and revealed composition-dependent fractionation effects. Py-GC-MS provided structural fingerprints of film components and putatively annotated nitrogen-containing compounds indicative of chitosan presence. Overall, these results demonstrate that the 1:1 films can be considered viable, multifunctional, and soil-friendly alternatives to conventional plastic mulches for sustainable agriculture. Full article

Quaternary ammonium compounds (QACs) have dominated biocidal practice in cultural heritage conservation for decades, yet growing evidence of environmental persistence, aquatic ecotoxicity, and antimicrobial resistance induction has prompted the search for safer alternatives. Essential oils (EO) have emerged as promising bio-based biocides, though their environmental performance has rarely been quantified through rigorous life cycle approaches. This study presents a comparative Life Cycle Impact Assessment (LCIA) of EO-based and QAC-based biocidal formulations across representative conservation scenarios, following ISO 14040/14044 standards and the Environmental Footprint 3.1 methodology with USEtox^® 2.1 characterization factors. Three complementary functional units were employed: formulation-based, surface-based, and intervention-based. The results reveal a fundamental trade-off: EO-based systems exhibit 81% higher climate change impacts but 82–89% lower human toxicity and freshwater ecotoxicity impacts compared to QAC-based systems. Surface-normalized comparisons reduce the climate gap to 32%, while toxicity advantages remain robust across all sensitivity scenarios. Monte Carlo analysis confirms the robustness of toxicity findings (p > 99%), whereas climate comparisons remain scenario-dependent. These findings support context-dependent adoption of EO-based biocides in conservation practice and demonstrate that EO-related climate impacts are technically mitigable, while QAC toxicity is intrinsic to their molecular structure. Full article

This study focuses on the machinability optimization of bio-waste corn husk fiber–reinforced epoxy composites during drilling, with the objective of minimizing delamination and improving hole quality required for mechanical fastening applications. While natural fiber composites have been widely investigated, systematic statistical optimization of drilling parameters for corn husk fiber composites remains limited. The novelty of this work lies in identifying the dominant drilling parameter and establishing a clear damage-control strategy using a Taguchi L16 design coupled with ANOVA. Drilling experiments were conducted by varying spindle speed (1000, 1500, 2000, and 2500 rpm), drill diameter (6, 8, 10, and 12 mm), feed rate (00.05, 0.10, 0.15, and 0.20 mm/rev), and point angle (90°, 100°, 110°, and 120°). The results show that the drill diameter is the governing factor affecting delamination, contributing 73.52% of the total variation, followed by spindle speed (22.68%), whereas feed rate (3.14%) and point angle (0.38%) have minimal influence. The optimal condition (2500 rpm, 6 mm drill diameter, and 0.05 mm/rev feed rate) produced the lowest delamination and improved surface integrity. Microscopic observations confirmed reduced fiber pull-out and matrix cracking under these conditions. The main advantage of the proposed approach is the clear identification of parameter priority, enabling the industry to control drilling damage by primarily selecting appropriate drill diameter and spindle speed. The findings provide practical machining guidelines for the use of corn husk fiber composites in lightweight panels, automotive interior parts, and secondary structural components where reliable bolted joints are required. Full article

Circular economy (CE) decouples value creation from virgin resource use and waste in the medical device sector, which faces stringent patient-safety, quality, and regulatory obligations. Green Additive Manufacturing (AM) offers a precise, digitally driven route to implement CE through dematerialization, on-demand localized production, topology optimization, and material circularity. In this study, a comprehensive CE framework is tailored to medical device manufacturing that integrates eco-design, material circularity, remanufacturing, and regulatory compliance across the product life cycle. Methods include an International Organization for Standardization (ISO) 14040/44-aligned life cycle assessment, process energy metering, sterilization-compatibility studies, mechanical/biocompatibility verification to relevant standards, and a techno-economic/circularity analysis with Monte Carlo uncertainty quantification. Three case studies are explored using bio-based PA11 (selective laser sintering), recycled polyethylene terephthalate glycol (fused deposition modeling), and low-volatile organic carbon biocompatible photopolymer (stereolithography): (1) a patient-specific wrist orthosis, (2) a dental surgical guide, and (3) a single-use catheter Y-connector. Results indicate 38–68% reductions in embodied greenhouse-gas emissions, 22–54% energy savings per functional unit, and up to 80% mass recapture through in-process powder/runner reuse while maintaining clinical performance and regulatory conformity. Design-for-circularity patterns (DfC) were created for DfDisassembly, DfSter, DfTraceability, DfUpgrade, and DfPowder-Loop and provide a governance architecture combining ISO 13485 QMS, ISO 10993 biological evaluation, the European Union’s Medical Device Regulation (Regulation (EU) 2017/745), and the United States Food and Drug Administration’s guidance on Additive Manufactured (3D-printed) medical devices, guidance with unique device identification for closed-loop returns. The paper concludes with an Industry 5.0 roadmap for hospital-proximate micro-factories, materials passports, and digital product passports enabling verified circular flows at scale. Full article

Smart healthcare is rapidly emerging as a transformative paradigm, enabling simultaneous health monitoring, therapeutic intervention, and early prediction of disease onset. In this context, electrochemical monitoring systems have attracted growing interest due to their cost-effectiveness, ease of operation, miniaturization and compatibility with wearable platforms. Accordingly, conductive hydrogel-based electrochemical (bio)sensors have gained significant attention for health monitoring owing to their soft mechanical properties, high water content, excellent biocompatibility, and ability to form intimate, conformal interfaces with biological tissues. Their three-dimensional polymeric networks facilitate efficient ion transport and mechanical flexibility, making them particularly suitable for wearable and noninvasive sensing and monitoring applications. However, the intrinsically limited conductivity and catalytic activity of pristine hydrogels often constrain their electrochemical performance. To overcome these limitations, functional nanomaterials such as metal–organic frameworks (MOFs) and MXene (MX) nanosheets have been increasingly integrated into hydrogel matrices to enhance conductivity and electrochemical activity. This review provides a comprehensive and critical comparison of recent advances in MOF- and MX-integrated conductive hydrogels for electrochemical health monitoring. In addition to material design strategies and sensing performance, emerging trends in data-driven sensing aimed at improving signal interpretation and multi-analyte discrimination are systematically discussed. Key challenges related to long-term stability, biocompatibility, scalability, and intelligent system integration are critically assessed, and the future potential of these platforms within closed-loop architectures is highlighted, paving the way for next-generation conductive hydrogel-based electrochemical sensors in smart healthcare applications. Full article

This paper presents the design and experimental evaluation of a bio-inspired gecko robot, focusing on mechanical design, vision-based obstacle perception, and rhythmic locomotion control as enabling technologies for future obstacle avoidance in complex environments. The robot features a 17-degrees-of-freedom mechanical structure with a flexible spine and multi-jointed limbs, providing a physical basis for adaptive locomotion. For perception, a custom obstacle detection dataset was constructed from the robot’s onboard camera view and used to train a YOLOv5-based detection model. Experimental results show that the trained model achieves a mean average precision (mAP) of 0.979 and a maximum F1-score of 0.97 at an optimal confidence threshold, demonstrating reliable real-time obstacle perception under diverse indoor conditions. For motion control, a central pattern generator (CPG) based on Hopf oscillators is implemented to generate rhythmic locomotion. Experimental evaluations confirm stable diagonal gait generation, with coordinated joint trajectories oscillating at 1 Hz. The flexible spine exhibits periodic lateral deflection with peak amplitudes of ±15°, ±10°, and ±8° across spinal joints, enhancing locomotion continuity and turning capability. Physical robot experiments further demonstrate smooth straight-line crawling enabled by the coupled limb–spine motion. While visual perception and CPG-based locomotion are experimentally validated as independent subsystems, their real-time closed-loop integration is not implemented in this study. Instead, this work establishes a system-level framework and experimental baseline for future perception–motion coupling, providing a foundation for closed-loop obstacle avoidance and autonomous navigation in bio-inspired gecko robots. Full article

European bioeconomy policies stress the need for responsible, efficient feedstock use and timely, comprehensive data on ecosystems and bioeconomic activities. This paper addresses the data gap by: (i) providing holistic county-level (sub-NUTS3) biomass maps for the Republic of Ireland (RoI), covering primary feedstocks (PFs) and secondary feedstocks (SFs, i.e., by-products and waste); (ii) identifying feedstock uses during the study period. In total, 221 feedstocks were mapped: 85 solid PFs (approx. 43 million tonnes dry matter (tDM) nationally) and 136 solid SFs (approx. 6 million tDM nationally), plus 6 liquid PFs (approx. 18 thousand million m³ nationally) and 8 liquid SFs (approx. 39 thousand million m³ nationally). The mapping indicates that environmentally sustainable bio-based value chains (BBVCs) requiring large amounts of solid or liquid SF should prioritise processing sites near major feedstock sources in the southeast and southwest of the RoI. The northwest and east coast have the lowest availability, while the west and midlands have the most variety in quantity and type of feedstock. Counties with abundant feedstocks do not necessarily have high feedstock diversity, except for Cork. Granular sub-NUTS3 mapping of quantities and fate provides a powerful foundation for future feedstock strategies and empowers stakeholders to design innovative BBVCs. Full article

This study investigates how natural fillers of different origins and morphologies influence the structural, thermal, rheological, and mechanical properties of thermoplastic elastomers (TPEs). Two series of materials were prepared: one based on a biobased matrix, poly(butylene 2,5-furandicarboxylate)-block-poly(tetramethylene oxide) (PBF-PTMO), and one based on a petroleum-derived matrix, poly(butylene terephthalate)-block-poly(tetramethylene oxide) (PBT-PTMO). Both series incorporated a range of natural modifiers, i.e., lignocellulosic fibers and ground fractions of Arundo donax L., cyanobacterial biomass (Spirulina platensis), and silica-rich mineral dust originating from volcanic stone quarries. The materials were obtained via melt blending, while the reference matrices (neat block copolymers) were synthesized through melt polycondensation. The chemical structure and limiting viscosity number (LVN) of the neat matrices were confirmed, while differential scanning calorimetry (DSC) provided insight into their morphology and phase composition. Scanning electron microscopy (SEM) was employed to evaluate the morphology and distribution of the modifiers within the polymer matrices. To assess how the fillers influenced processing windows and performance, thermogravimetric analysis (TGA), oscillatory rheological measurements, and tensile testing were performed. The results provide insight into structure–property relationships governing natural filler–TPE interactions and support the development of more sustainable elastomeric composites with tailored performance. Full article

Molecularly imprinted polymers (MIPs) are synthetic materials with highly selective recognition properties and are widely studied for applications in separation, sensing, catalysis, and biomedical analysis. However, conventional MIP synthesis often relies on toxic solvents and reagents, causing environmental and sustainability concerns. This review critically examines recent advances in the green synthesis of MIPs, focusing on strategies aligned with green chemistry principles. Emphasis is placed on the use of environmentally less toxic solvents, as well as bio-based and less hazardous functional monomers and crosslinkers. Emerging polymerization techniques, such as microwave-assisted, photochemical, and solvent-free approaches, are also discussed. The impact of green synthetic routes on the structural, physicochemical, and recognition properties of MIPs is analyzed, highlighting both benefits and current limitations. Finally, key challenges and future perspectives for the development of sustainable MIPs are outlined. Full article

In this study, we develop bio-epoxy composites incorporating bio-oils obtained from the pyrolysis of almond and walnut shells at 400 °C and 600 °C, with the aim of evaluating their potential as renewable precursors for epoxy resin modification. The influence of pyrolysis temperature on bio-oil yield and chemical composition is examined to identify phenolic-rich fractions relevant to epoxy curing. Bio-oil production increased with temperature, reaching 40.46% for walnut shells and 36.84% for almond shells at 600 °C. Chemical analysis revealed that aromatic compounds, particularly phenolics, were the major constituents associated with epoxy curing reactivity. For walnut hulls, the total aromatic fraction increased from 30.4% at 400 °C to 35.2% at 600 °C, while almond hulls showed an increase from 23.8% to 26.1% over the same temperature range. Incorporation of bio-oil into the epoxy matrix promoted three-dimensional network formation through reactions between epoxy groups and the functional moieties present in the bio-oil, resulting in a higher cross-linking degree, Young’s modulus, and tensile strength. However, compared to neat epoxy, the bio-oil-modified systems exhibited reduced storage modulus (E′) and glass transition temperature (Tg), attributed to the plasticizing effect of lighter oxygenated species. Overall, although bio-oil incorporation decreases Tg and cross-linking degree, it still provides a viable pathway toward partially bio-based epoxy resins with enhanced stiffness and competitive mechanical performance. Full article

The increasing demand for high-performance composites has driven the need for sustainable alternatives to conventional petroleum-based resins. This research introduces a novel glycerol-derived bio-epoxy resin and investigates the effect of catalyst concentration on its curing behaviour, network structure, and thermomechanical performance. Four catalyst concentrations were evaluated using differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), and dynamic mechanical analysis (DMA) combined with tensile, flexural, and compression testing. DSC results revealed that increasing the catalyst concentration significantly lowered the curing activation energy, shifting the exothermic peak temperature from 194.8 °C to 145.2 °C. DMA revealed that the glass transition temperature (T_g), crosslinking density, and stiffness consistently increased up to an optimal catalyst concentration, reaching a maximum T_g of 109.0 °C. Further increases in catalyst content led to slight reductions in T_g and crosslink density due to the formation of a heterogeneous network. The optimal concentration enhanced tensile and compressive strength by 32.8% and 9.3%, respectively. At excessive catalyst concentration, strength properties deteriorated despite increased material rigidity. These findings confirm the critical role of catalyst in governing polymerisation kinetics and network structure, demonstrating that an optimal catalyst percentage is essential for maximising strength and durability, making the bio-epoxy a viable, high-performance alternative for advanced composite manufacturing. Full article

Poly(lactic acid) (PLA) is widely used in food-contact applications due to its bio-based origin, compostability, and transparency; however, its limited resistance to thermo-oxidative degradation remains a challenge for applications involving repeated thermal exposure. The moderate but repetitive heating conditions commonly encountered during food use and pre-recycling stages were analyzed for the samples filled with algal biomass and rosemary extract, additives accepted for use in the food industry. In this context, the present study introduces a comparative and application-driven approach by evaluating the effect of food-grade fillers—rosemary extract, spirulina biomass, and kelp biomass—incorporated at low loadings (0.5–3 wt%) on the thermal and oxidative behavior of PLA subjected to repeated heating at 80 °C. The presented results show algal biomasses as multifunctional fillers and benchmark their performance against a well-established natural extract. By combining DSC, FTIR, and chemiluminescence analyses, the study aims to clarify whether such bio-fillers act as stabilizing or destabilizing factors under realistic service-life thermal stress. This strategy provides insight into the suitability of algae-based fillers for food-contact PLA materials from both performance and recyclability perspectives. Full article

Flapping-wing micro air vehicles (FWMAVs) have gained increasing attention due to their manoeuvrability, low acoustic signature, and suitability for confined or cluttered environments. Despite considerable progress, existing reviews treat actuation mechanisms and mechanical transmissions in isolation, leaving a gap in the comparative assessment of pulley-based and alternative flapping systems. This study provides a comprehensive and quantitative synthesis of the literature on FWMAV mechanical architectures, with particular emphasis on pulley-driven transmissions used in platforms such as the Nano Hummingbird and the Robotic Hummingbird. A structured review methodology was applied, incorporating a systematic database search, extraction of performance parameters, and cross-platform comparison of flapping frequency, lift generation, power consumption, lift-to-weight ratio, and material choices. The analysis identifies consistent scaling trends across motor-driven, piezoelectric, and hybrid actuation families and highlights the efficiency and stroke-amplification advantages of pulley-based mechanisms for centimetre-scale hovering MAVs. The review also identifies unresolved challenges, including durability of transmission materials, standardisation of performance metrics, and the need for high-fidelity aerodynamic characterisation. Overall, this work offers an integrated framework for understanding the trade-offs among actuation and transmission strategies and provides a roadmap to guide future research and the practical development of next-generation FWMAVs. Full article