Search Results (2,795)

Developing eco-friendly metal oxide nanoparticles (NPs) with plant-based reducing and stabilizing agents offers a sustainable alternative to traditional chemical methods. Nonetheless, the detailed mechanisms by which phytochemicals influence NPs formation, antimicrobial properties, and cytocompatibility remain poorly understood, especially in systems mediated by Vaccinium. This study aimed to synthesize TiO₂ NPs and ZnO NPs using Vaccinium corymbosum (blueberry) extract, analyze their structural and surface characteristics, assess their antimicrobial effectiveness and cytotoxicity, and explore potential molecular mechanisms through computational docking. ZnO NPs were produced via alkaline precipitation (pH 12) from ZnCl₂, while food-grade TiO₂ was mixed with blueberry extract. A comprehensive characterization was carried out using techniques like X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy, transmission and scanning electron microscopy (TEM/SEM), dynamic light scattering (DLS), and high-performance liquid chromatography (HPLC) for polyphenol profiling. The antimicrobial activity was tested against Escherichia coli and Salmonella Typhimurium, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. Cytotoxicity was assessed using Gallus gallus domesticus leukocytes and Artemia salina bioassays, and molecular docking simulations were performed to examine polyphenol interactions with the bacterial DNA gyrase subunit B (GyrB). XRD analysis confirmed the presence of wurtzite ZnO (with a crystallite size of 18.2 nm) and anatase TiO₂ (12.8 nm after functionalization). HPLC identified key polyphenols, including quercetin, cyanidin, malvidin, and cyanidin-3-glucoside, with patterns indicating stronger adsorption onto TiO₂ NPs surfaces. ZnO NPs showed higher antimicrobial effectiveness (>90% inhibition at 2 mg/mL; MIC 0.5–1 mg/mL) compared to TiO₂ (72% inhibition at 16 mg/mL; MIC 8–16 mg/mL). Cytotoxicity results indicated concentration-dependent effects. Molecular docking simulations revealed favorable binding energies (−6.2 to −8.4 kcal/mol) for blueberry polyphenols with GyrB, suggesting potential synergistic antimicrobial effects and ROS production. The study highlights a successful green synthesis of bioactive TiO₂ NPs and ZnO NPs using Vaccinium corymbosum extract, where polyphenol surface functionalization enhances both colloidal stability and biological activity. This comparative research offers mechanistic insights into how polyphenol-coated NPs work and supports the development of eco-friendly antimicrobial oxide nanomaterials. Full article

The use of non-biodegradable plastic food packaging materials has become a major environmental concern. These plastics release chemicals and microplastics during degradation, harming wildlife and entering the food chain, posing risks to both environmental and human health. This study aimed to evaluate electrospun poly(vinylpyrrolidone) (PVP) mats incorporating natural antibacterial Thymus vulgaris L. extract (TE) and natural crosslinker citric acid (CA) as alternative food packaging materials. Packaging mats with TE and/or CA combinations in PVP were evaluated for their structural, chemical, optical, and shelf-life-enhancing effects on blueberries. The results show that dissolving PVP in TE extract and adding CA in PVP ethanol-water or TE-based solutions significantly affected the viscosity and conductivity of the electrospinning solutions, thereby influencing the morphology of electrospun mats. FTIR analysis confirmed the incorporation of TE into the polymer and indicated CA induced hydrogen bonding, interactions that may reduce the polymer chain mobility and increase the brittleness of the electrospun mat. In tests with blueberries, it was estimated that the commonly used traditional food film minimized blueberry weight loss, whereas the porous electrospun PVP and PVP/TE mats allowed greater moisture release and preserved better visual quality by reducing wrinkling and dehydration. Overall, electrospun PVP-based mats functionalized with TE show promise as sustainable food packaging materials that balance moisture management with product appearance. Full article

Pycnospatha is a small and poorly known genus of Araceae distributed in Indochina and currently comprising only two accepted species. During botanical surveys in Si Sa Ket Province, eastern Thailand, an unusual population of Pycnospatha was discovered in a dry dipterocarp forest and found to differ from both P. arietina and P. palmata. Here, we describe this plant as a new species, Pycnospatha phanomdongrakensis. The new species is distinguished by a combination of characters, including a slender habit, shorter petiole and peduncle, a medium-sized spathe, a short and dense spadix, a distinctly curved style directed toward the apex of the spadix, a geophilous and ovoid infructescence, obovate berries, and asymmetrically ovoid seeds. The new taxon is currently known only from a single population in the Phanom Dong Rak mountain range. A preliminary conservation assessment is provided, and the species is treated as Critically Endangered (CR) following IUCN guidelines. An identification key to all species of Pycnospatha is also presented. The discovery of this new species highlights the continuing importance of field-based taxonomy in revealing overlooked aroid diversity in the seasonally dry forests of eastern Thailand. Full article

Honeysuckle berries (Lonicera caerulea) represent a valuable source of bioactive compounds, primarily flavonoids, and iridoids. This study compared the chemical composition and in vitro antioxidant and antidiabetic properties of resin-purified extracts from ripe, unripe, and unripe lactofermented honeysuckle berries. Polyphenols and iridoids were identified using UPLC-ESI-qTOF-MS/MS and quantified using HPLC-PDA. A total of 6 anthocyanins, 7 phenolic acids, 9 flavan-3-ols, 8 iridoids, 8 flavonols, 3 flavones, and 1 flavanonol were identified in the extracts. The extract from ripe fruits was characterized by a high cyanidin glycoside content (273.59 mg/g) and high iridoid content (138.30 mg/g). The amount of individual iridoids varied among the extracts, with the highest level of loganic acid detected in the unripe fruit extract (39.42 mg/g) and the highest level of sweroside in the ripe fruit extract (55.59 mg/g). Phenolic acid content was approximately twofold higher in extracts from unripe and fermented fruits compared with ripe fruit extracts, suggesting a decrease during ripening, while fermentation did not significantly affect phenolic acid content. Among flavonols, quercetin and isorhamnetin derivatives were identified, with quercetin 3-O-rutinoside being the predominant compound in all extracts. The ripe fruit extract exhibited the strongest radical scavenging activity (in ABTS and DPPH assays), ferric ion-reducing power (FRAP), and α-amylase inhibition, while all extracts exhibited comparable α-glucosidase inhibition. These findings indicate that L. caerulea extracts, especially from ripe fruits, are a rich source of biologically active compounds with potential relevance for managing oxidative stress and hyperglycemia. Full article

Climate warming in Mediterranean vineyards accelerates grape ripening and increases the incidence of sunburn and berry shriveling, leading to imbalances in grape composition and wine quality. This study evaluated the combined effects of a non-positioned training system (asymmetric sprawl) and foliar application of kaolin particle film on vine microclimate, agronomic performance and wine aroma profile in a Syrah cv. vineyard under warm conditions. Vine canopy temperature was monitored by UAV thermography at veraison and harvest, while grape damage, yield components and vegetative balance were assessed at harvest. Wines obtained from each treatment were analysed for chemical composition, volatile compounds and sensory attributes. Kaolin application significantly reduced canopy temperature, particularly under water-limited conditions at veraison (up to 1.9 °C), and the combination with sprawl training decreased the proportion of sunburnt and shrivelled clusters. These microclimatic modifications were associated with higher ethanol content, improved colour intensity and increased total polyphenol index in wines. The combined strategy also enhanced the concentration of key aroma compounds, especially terpenes and fruity esters, resulting in higher values of citrus, floral and fruity aromatic series. Sensory evaluation confirmed a better overall appreciation of wines produced from vines managed with both practices. Overall, the integration of canopy architecture modification and reflective particle film represents an effective strategy to mitigate heat stress effects in warm viticultural regions, improving grape physiological performance and contributing to the preservation of wine aromatic quality under climate change scenarios. Full article

Controlling spin dynamics conventionally requires external magnetic fields, strong electric bias, or material-specific spin–orbit interactions, while the temporal reference frame remains fixed. Here we introduce curved-time spintronics, a framework in which a synthetic lapse field, implemented through GHz surface-acoustic-wave (SAW) modulation, reshapes the effective flow of time experienced by spinor, magnonic, and photon–spin degrees of freedom. Using a curved-time Schrödinger–Pauli model, we show that it renormalizes the Larmor frequency, modifies SOC-driven splittings, and produces helicity-dependent spin precession under circularly polarized excitation. Strikingly, a spatial lapse gradient induces a Hall-like transverse drift even when in the absence of any external electric field or intrinsic Berry curvature, demonstrating that time geometry alone can generate transverse transport. Time-domain simulations confirm curvature-driven Hall response across graphene, carbon nanotubes, and generic Dirac platforms, establishing a material-agnostic, field-free mechanism for transverse spin manipulation. We further predict curvature-dependent spin diffusion, temporal magnon focusing, and helicity-selective entanglement generation, and propose pump–probe detection via ultrafast Kerr rotation synchronized to SAW-driven lapse modulation. These results position engineered time geometry as a new spintronic control axis, enabling Hall-like effects, spin transport, and chiral phase manipulation without relying on intrinsic material properties, magnetic fields, or electric gating. Full article

The popularity of herbal remedies is on the rise, but this often comes at the expense of general knowledge about non-native species. The frequency and versatility of the use of medicinal plants does not depend on their origin, while the use of species with proven efficacy is much more intensive. The most abundant plant families, according used taxa and use records, are Asteraceae, Rosaceae and Lamiaceae. The greatest consensus is on the choice of taxa suitable for the treatment of bloating/flatulence and diarrhea, most commonly treated with Carum carvi L. fruit tea and dried or fresh berries of Vaccinium myrtillus L., respectively. The most popular species for treatment of digestive disorders are Artemisia absinthium L. and Matricaria chamomilla L. The use of different taxa for the treatment of digestive disorders in Lithuania varies considerably for a number of reasons, one of which is the uneven distribution of medicinal plant species in the study areas. Full article

Small and Medium-sized Enterprises (SMEs) are central to the industrial fabric of Sub-Saharan Africa (SSA). Yet, they confront increasing demands to implement sustainability practices originating from institutional contexts markedly different from their own. Existing research has tended to neglect the cultural and institutional negotiations inherent in this process, often framing sustainability adoption as a technical or compliance-oriented exercise rather than as a multifaceted cultural adaptation. This study proposes and empirically examines the concept of sustainability acculturation—the process by which firms align global sustainability norms with local business cultures. Drawing on Institutional Theory, the Resource-Based View, and Berry’s Acculturation Model, we present a context-specific framework, tested using a sequential explanatory mixed-methods approach: survey data from 284 manufacturing SMEs across six SSA countries, followed by 24 semi-structured interviews. Structural equation modeling reveals that international market pressure and owner–manager values are direct drivers, whereas local regulatory pressure exhibits only a weak association with deep cultural integration. Managerial commitment and organizational learning mediate these relationships, while Ubuntu values enhance social sustainability integration, and institutional voids diminish regulatory effectiveness. The model accounts for 57% of the variance in sustainability acculturation. Findings show that SSA SMEs employ distinct acculturation strategies—Integration, Assimilation, Resilient Adaptation, and Decoupling—shaped by the interplay of external pressures, internal capabilities, and contextual conditions. The study underscores the importance of culturally attuned, context-specific interventions for sustainable industrial development in SSA. Full article

The Mediterranean-DASH Intervention for Neurodegenerative Delay (MIND) diet has emerged as a promising dietary pattern associated with reduced Alzheimer’s disease (AD) risk, supported by growing evidence that both diet and the gut microbiota are modifiable contributors to disease development and progression. Observational studies have linked higher MIND diet adherence to lower AD incidence and slower cognitive decline, with certain comparative analyses reporting stronger associations with cognitive outcomes than those observed for the parent Mediterranean or DASH diets. Developed specifically to support cognitive health, the MIND diet emphasizes leafy green vegetables, berries, and olive oil while restricting butter, cheese, fried foods, sweets, and red meat. While these features suggest a biologically plausible basis for neuroprotection, the underlying mechanisms remain incompletely defined. The microbiota–gut–brain axis offers a potential mechanistic framework, as diet is a major determinant of gut microbiota composition and microbiota-derived metabolites that may influence brain function and AD-related pathways. However, direct evidence characterizing MIND diet-specific effects on the gut microbiota remains limited, with most mechanistic insights derived from related dietary patterns or individual dietary components. Accordingly, this review synthesizes evidence from these related dietary patterns and key MIND components to propose a conceptual framework linking the MIND diet, the gut microbiota, and AD risk, while highlighting priorities for future research. Full article

To clarify the genetic background and biological characteristics of grape germplasm resources and provide theoretical support for germplasm innovation and new-variety breeding, we conducted systematic morphological identification and SSR molecular-marker analysis on 38 core grape germplasms (29 fresh-eating cultivars, 1 local cultivar, and 8 wild germplasms) from the National Southeast Mountainous Crop Germplasm Repository (Jiangxi·Yichun) and other regions. For morphological identification, 14 quantitative traits and 5 descriptive traits of leaves, floral organs and fruits were determined in strict accordance with the NY/T 2932-2016 Descriptors for Grape Germplasm Resources. For SSR molecular-marker analysis, eight pairs of internationally universal core primers were used for PCR amplification and fluorescence detection referring to the NY/T 3640-2020 Identification of Grape Cultivars Using SSR Markers, and genetic diversity analysis was conducted on 11 local and wild grape germplasms. The results revealed abundant phenotypic diversity among the tested germplasms: the functional leaves of cultivars were predominantly pentagonal and cuneate, while those of wild germplasms were mostly reniform and cordate, with 3–5 lobes for most germplasms; all germplasms were hermaphroditic, except for two wild accessions with unisexual flowers. Significant variations were observed in fruit traits, with the coefficient of variation (CV) of cluster weight and berry weight reaching 67.64% and 50.53%, respectively. The genetic plasticity of weight-related traits was much higher than that of shape- and length-related traits, and the average Shannon–Wiener index (H′) of 19 morphological traits was 3.47, indicating a high level of overall phenotypic diversity. SSR analysis showed that the eight primer pairs amplified a total of 42 genotypes (5.25 per primer pair on average). The population had a mean observed number of alleles (Na) of 5.28, a mean effective number of alleles (Ne) of 7.25, and a mean polymorphism information content (PIC) of 0.74, demonstrating rich genetic diversity and high polymorphism of the tested loci. Cluster analysis divided the 11 local germplasms into four groups, which clearly reflected the genetic relationships among them, and genetic admixture was found in some germplasms due to unclear introduction traceability. In this study, fresh-eating grape cultivars suitable for the climatic conditions of Jiangxi Province were screened, the utilization value of local germplasm resources was clarified, and a two-dimensional evaluation system based on phenotypic traits and SSR molecular markers was constructed. The findings provide basic data and a scientific basis for the precise evaluation, elite gene mining, and new-variety breeding of grape germplasm resources in Jiangxi Province. Full article

Gluten-free cakes formulated solely with rice flour frequently exhibit limited volume, weak internal structures, and rapid quality deterioration. This study investigated the effects of replacing rice flour with 0, 3, 6, 9, and 12% goji berry powder (GBP) in gluten-free cupcakes. Physical properties, texture profile, crumb porosity, crust and crumb color, flour pasting behavior, and antioxidant properties were evaluated. Moderate GBP addition improved cupcake quality, with the 6% treatment showing the greatest height (45.17 mm) and specific volume (3.64 cm³/g), the lowest hardness (327.50 g), the highest springiness (9.25 mm), and the largest average pore area (0.42 mm²). In contrast, higher substitution levels (9–12%) increased moisture and reduced baking loss but caused a decline in specific volume and a marked increase in hardness. GBP progressively darkened the product, especially the crumb, while significantly enhancing total phenolic content and DPPH radical-scavenging activity from 55.46 to 67.36 mg gallic acid equivalents (GAE)/100 g and from 4.85 to 15.08 mg ascorbic acid equivalents (AAE)/100 g, respectively. Monotonic decreases in peak, trough, final, and setback viscosities were observed, indicating reduced starch swelling and retrogradation tendencies as the GBP level increased. GBP at 6% showed the most balanced overall performance, while 12% maximized the antioxidant response at the expense of structural quality. Full article

Grapevine (Vitis vinifera L.) is an economically important fruit crop cultivated worldwide. However, its production and fruit quality are severely constrained by powdery mildew (Erysiphe necator) and Botrytis bunch rot (Botrytis cinerea) diseases. Increasing concerns regarding chemical fungicide resistance and environmental sustainability highlight the urgent need to develop alternative and more sustainable disease management strategies. This study assessed the field efficacy of Pseudomonas protegens-based formulations (TANIRI^® WP at 1 g·L⁻¹ and MaxGrowth at 1 mL·L⁻¹) within an integrated disease management program in cv. Chardonnay. Defense-related gene expression analysis revealed that biological treatments predominantly up-regulated pr1, pr2, and pr10 in both leaves and berries. In contrast, the chemical inducer acibenzolar-S-methyl (ASM) triggered earlier but less consistent induction of pr1 and pr2, alongside transient activation of pal and lox9. Repeated field applications of P. protegens formulations moderately reduced the severity of Botrytis bunch rot (20.89%) and powdery mildew (6.14%), though control levels remained below conventional sulfur/Bacillus subtilis-based treatments (30.04% and 13.56%, respectively). Overall, these findings suggest that biological inducers could complement conventional management practices for grapevine health. In particular, P. protegens may act mainly by systemically inducing host defense responses and partially suppressing pathogen development under field conditions. Full article

The present study was conducted to evaluate the effect of biodegradable mulches and microbial biostimulants (BMs) on processing tomato (Solanum lycopersicum L.) cv. Pietra rossa. A black Mater-Bi^®-based film (MB), a black paper film (Np), a light-brown paper film (Sp), and bare soil (BS) were factorially combined with Trichoderma harzianum M10, T. afroharzianum T22, and the metabolite 6-pentyl-α-pyrone (6PP). The mulching practice influenced soil temperatures that were lower under all types of mulching from the end of May up to the first week of June, but, by contrast, they were greater than those of the BS just under MB for the entire next period. Mulching and BM application increased the marketable fruits (by 26% MB vs. BS and by 27% T22 vs. untreated control (CTRL)) and reduced rotten fruits (by 46% Np vs. BS and by 31% T22 vs. CTRL). Lycopene increased under MB and Sp (not Np) vs. BS and with 6PP and T22 (not M10) vs. CTRL, while ascorbic acid was unaffected by mulching and increased only with T22, and total phenols decreased with mulching but were unaffected by BMs. Tomato berry metabolism is treatment-dependent: 6PP consistently separated from the microbial-based treatments (M10 and T22) across all mulching types. Overall, it demonstrated a consistent and distinct impact on a subset of metabolites (i.e., phenolic acids and steroidal glycoalkaloids) across different mulching conditions compared to the microbial treatments, which exhibited a mulch-dependent accumulation. Full article

The coffee berry borer (Hypothenemus hampei) is the primary pest of coffee crops worldwide. Sustainable management strategies increasingly rely on the integration of biological control and interventions activated by population thresholds. In this work, a comparative framework based on dynamical systems is presented, integrating three complementary mathematical models to analyze different management strategies for the coffee berry borer. First, a biologically structured three-dimensional model describes the interaction between adult and immature borers and predatory ants. Second, a two-dimensional formulation allows the maximum per capita consumption rate of the predator to be studied as a bifurcation parameter, identifying critical parameter values that delimit regions of coexistence or effective pest control. Finally, a piecewise-smooth dynamical system incorporates ethological control activated when infestation exceeds a predefined threshold, whose effectiveness depends on the capture intensity associated with the traps. Using stability theory, bifurcation analysis, and techniques from piecewise-smooth dynamical systems, parametric regions associated with persistence, coexistence, or significant pest reduction are characterized. The results show that biological control alone may be insufficient if a predation threshold is not exceeded, whereas its combination with early threshold-based interventions considerably enlarges the dynamical regions favorable to producers. This study provides a dynamical interpretation of the agricultural concept of intervention threshold and offers a quantitative framework to strengthen integrated management and the sustainability of coffee production. Full article

Sensitivity to the bitterness of 6-n-propylthiouracil (PROP) is controlled by variations in the TAS2R38 gene. This phenotype is often used as a marker for individual differences in taste perception. Previous findings show that PROP taster status is associated with differences in the salivary microbiome. It is well known that diet and environmental factors influence the risk of oral disease, but there is far less evidence showing how genetic differences play a role. Forty-seven young, healthy, PROP taster-classified adults rinsed with a cranberry polyphenol extract (CPE) oral rinse (0.75 g/L CPE powder in spring water) twice daily for 11 days. Saliva was collected pre- and post-intervention for microbiome analysis using shotgun metagenomic sequencing. At the same time points, participants evaluated two astringent juices (cranberry and aronia berry) for key attributes. At baseline, PROP taster groups differed in their salivary microbiome compositions, but post-intervention, the groups had more similar bacterial compositions. Post-intervention, non-tasters showed decreases in the relative abundance of 15 bacterial species, including a significant reduction (p = 0.037) in Eikenella corrodens, which is one bacterium, among several others, involved in oral biofilm formation. Additionally, after the intervention, sourness was reduced, and overall liking increased significantly for aronia juice. Oral dysbiosis, a risk factor for oral disease, may be controlled by bactericidal mouthwashes. Our results suggest that CPE, a natural alternative to traditional bactericidal rinses, may selectively target pathobionts while preserving salivary microbiota diversity. CPE might also provide greater benefits to non-tasters, who are at greater risk for oral disease. Full article