Search Results (707)

The inclusion of meat quality traits in breeding programs is a promising strategy to improve beef by selecting animals based on both growth and meat quality. This study aimed to estimate genetic parameters for average daily gain (ADG) and Warner–Bratzler shear force (WBSF), as well as to perform genome-wide association studies (GWAS) to identify genomic regions and transcription factor (TF) binding sites associated with both traits in Nelore cattle. Genetic parameters were estimated using a bi-trait Bayesian model, and GWAS identified key SNPs explaining over 1% of variance in genomic estimated breeding values. Candidate genes near these SNPs were annotated, TF binding sites predicted, and gene–TF networks constructed. Genetic estimates indicated moderate heritability for ADG, low heritability for WBSF, and a small negative genetic correlation between traits. Genomic regions contained 116 and 151 candidate genes for ADG and WBSF, respectively, with 35 shared between traits. Functional analyses highlighted MYBPC1 and PENK for WBSF, and GHRS and NPY for ADG. TF analysis identified 25 TFs, with 3 key ones highlighted. Gene–TF networks revealed candidates including CAPN1 and LTBP3 for WBSF, and CARM1 and GH1 for ADG. Shared candidate genes identified in the combined network provide valuable insights into the genetic architecture of growth and tenderness in Nelore cattle. Full article

Cultured meat is an emerging innovation with the potential to contribute to sustainable animal production by reducing the environmental and ethical challenges associated with conventional animal farming. In Romania, this new source of protein is viewed with both interest and scepticism, reflecting broader consumer uncertainties about food innovations. Market data indicates that Romania faces a significant deficit in animal protein supply (exceeding 65% for pork, as well as notable deficits in fish and beef), while also recording one of the largest overall trade deficits in pet food. In this context, the present study explored the openness of respondents in northeastern Romania to the consumption of cultivated meat, taking into account both personal diet and pet nutrition. The results showed that 33.2% of participants were fully willing to feed their pets cultured meat products, and 70% supported its introduction to the market, provided that regulatory frameworks ensure product safety and quality. Although Romania does not currently have the legal and technological infrastructure necessary to integrate cultured meat into the food system, these findings highlight the potential role of consumer acceptance in shaping future nutritional strategies aimed at sustainable, high-quality protein alternatives for humans and pets alike. Full article

The search for natural additives from underutilized halophytes and fruit by-products aligns with circular economy principles, addressing consumer demand for healthier and more sustainable alternatives to salt and synthetic antioxidants in foods. Salicornia ramosissima, a halophytic plant rich in minerals, and Malpighia emarginata (acerola), a fruit rich in bioactive compounds, were selected for their potential to enhance meat preservation while reducing reliance on conventional salt and chemical additives. This study evaluated the effects of replacing salt with S. ramosissima powder (1% and 2%) and adding acerola extract (0.3%) in Mertolenga D.O.P. beef hamburgers. Control, 1% salt, acerola, and salicornia formulations were analyzed over 10 days for the following: (1) microbial counts (mesophiles, psychrotrophics, Enterobacteriaceae, Pseudomonas spp., Brochothrix thermosphacta, lactic acid bacteria, fungi, Salmonella spp., and E. coli); (2) physicochemical parameters (pH, a_w, and CIE-Lab color); and (3) sensory attributes (odor, color, and freshness). Higher Salicornia concentrations negatively affected color (lower a* values) and sensory perception (darker appearance). Acerola extract improved color stability and delayed the development of off-odors, contributing to higher freshness scores throughout storage. No significant differences in microbial counts were observed between treatments. Overall, acerola and low-dose Salicornia showed potential as natural ingredients for meat preservation, with minimal impact on physicochemical and microbiological quality. These findings support the use of halophytes and fruit extracts in sustainable meat preservation strategies. Full article

Discrimination between normal (fresh/non-frozen) and frozen-thawed beef is crucial for ensuring food safety. This paper proposed a novel, non-destructive and real-time you only look once for normal and frozen-thawed beef discrimination (YOLO-NF) model using deep learning techniques. The simple, parameter-free attention module (SimAM) and the squeeze and excitation (SE) attention mechanism were introduced to enhance the model’s performance. A total of 1200 beef samples were used, with their images captured by a charge-coupled device (CCD) camera. In the model development, specifically, the training set comprised 3888 images after data augmentation, while the validation set and test set each included 216 original images. Experimental results on the test set showed that the YOLO-NF model achieved precision, recall, F1-Score and mean average precision (mAP) of 95.5%, 95.2%, 95.3% and 98.6%, respectively, significantly outperforming YOLOv7, YOLOv5 and YOLOv8 models. Additionally, gradient-weighted class activation mapping (Grad-CAM) was adopted to interpret the model’s decision basis. Moreover, the model was deployed on the web interface for user convenience, and the discrimination time on the local server was 0.94 s per image, satisfying the requirements for real-time processing. This study provides a promising technique for high-performance and rapid meat quality assessment in food safety monitoring systems. Full article

Using locally produced forage and agro-industrial by-products can reduce dependence on imported feed and competition for human food sources, while improving meat quality. However, the overall effect of this feeding strategy on global greenhouse gas emissions must be evaluated to provide a comprehensive assessment of sustainability. This study aimed to test whether replacing the conventional concentrate finishing diet with a total mixed ration (TMR) diet based on maize silage and brewer’s spent grains (BSG) would improve meat quality without compromising productive performance, carcass composition, and the carbon footprint (CFp) of finishing beef cattle. Twenty crossbred young bulls were randomly distributed among 4 pens and randomly allocated to 2 treatments: Control—a conventional diet based on commercial concentrate and wheat straw or TMR—a maize silage-based diet with BSG, concentrate, and straw. Dry matter intake and average daily gain were 13% and 15%, respectively, lower in the TMR treatment than in the Control treatment. Daily methane emissions were 59% higher in the TMR treatment. However, life cycle assessment results revealed no differences in the CFp, and the beef from TMR treatment achieved higher meat quality. In conclusion, a maize silage-based diet offers a cost-effective alternative to conventional diets, with a lower environmental impact and improved beef quality. Full article

Halophilic isolates were screened to mitigate postharvest losses caused by citrus canker disease in lemon fruits. Among all isolates, SWIS03, isolated from the Sambhar Salt Lake in Rajasthan, India, exhibited the highest exopolysaccharide production, with good stability and antibacterial activity against Xanthomonas citri. Isolate SWIS03 was identified as halophilic Bacillus licheniformis DET601. It produced a very high EPS content in optimized sterilized seawater-based minimal media fortified with 2.5% sucrose and 0.3% beef extract, which was purified through repeated deproteinization and Sephadex G-75 chromatography. HPTLC analysis of composition check indicated the presence of glucose, mannose, and galactose. FTIR analysis also confirmed the presence of sugar and bound water, as well as the presence of hydroxyl, amine, amide, and methyl groups. Rheological characterization revealed a pseudoplastic nature, making it suitable for uniform coating. EPS was reported to have bioactive properties, including antioxidant activity (84.7 ± 1.75% DPPH scavenging potential), antimicrobial activity against human pathogens, and a noncytotoxic nature, which are essential for use in edible coatings. The effect of EPS coating formulation on healthy lemon fruits resulted in shelf-life extension of up to 26.6 ± 1.14 days and 18.0 ± 1.41 days at 4 °C and 30 °C in coated lemons, respectively, as demonstrated by physiological parameters such as % weight loss, firmness, titratable acidity, and shelf life of lemons. Also, EPS coating preserved the quality of fruits in terms of phenolic compounds and Vitamin C content, and reduced lipid peroxidation during storage. Biocontrol potential of halophilic EPS coating on lemon fruits revealed an 86.50% and 68.64% reduction in % disease incidence compared to uncoated fruits at 4 °C and 30 °C, respectively. Similarly, a significantly lower disease incidence (46.80% at 4 °C and 67.03% at 30 °C) was also reported compared to paraffin-coated (positive control) lemons. Consequently, halophilic Bacillus licheniformis DET601 EPS is an effective coating material for citrus fruits to prevent canker disease in post-harvest settings for agricultural and food industry applications. Full article

This study introduced a new low-temperature storage method that applies an additional lower strength static electric field (SEF) under the condition of a static magnetic field (SMF) to investigate the impact of magneto–electric coupling on the supercooling degree and quality of beef. The results showed that 7 mT-1 kV performs the best (−5.8 °C); the ability of SMF to maintain supercooling is less affected by SEF. Moreover, on the 15th day, magneto–electric coupling (7 mT-1 kV) outperformed SMF (7 mT) alone by reducing beef pH by 0.27, decreasing total viable counts (TVC) by 0.87 log CFU/g, maintaining TVB-N at only 12.5 mg/100 g, and limiting oxidative change, calpain activity, and shear force variation. Magnetic resonance imaging revealed that magneto–electric coupling treatment stabilized the T₂ relaxation time in meat samples, effectively inhibiting immobilized water migration and promoting more uniform moisture distribution, highlighting its application potential as a low temperature preservation method. Full article

This research was carried out to assess the impact of daidzein supplementation on production performance, serum biochemical indexes, meat quality, and the transcriptome of the longissimus dorsi (LM) muscle in heat-stressed Jinjiang cattle. Twenty 20-month-old Jinjiang cattle (initial mean ± SE: 438 ± 34.6 kg of body weight) were randomly divided into two treatment groups (n = 10 per treatment): control treatment and daidzein treatment (1000 mg/kg concentrate). After a 100-day feeding trial (consisting of a 10-day adaptation period and a 90-day daidzein feeding period), blood and LM muscle samples were collected on day 100. Daidzein significantly increased the average daily dry matter intake (ADMI), the concentration of free fatty acid (FFA) and glutamic-pyruvic transaminase (ALT) in serum, and the marbling score of the LM muscle. Additionally, daidzein significantly decreased the concentration of total cholesterol (TC) and leptin in serum, along with the shear force and L* value of LM in heat-stressed Jinjiang cattle. The transcriptome analysis demonstrated that 238 differentially expressed genes (DEGs) were identified through differential expression analysis, among which 168 genes were downregulated and 70 genes were upregulated. The results of KEGG pathways showed that these DEGs were significantly enriched in pathways related to beef tenderness, including the FoxO signaling pathway, Notch signaling pathway, and regulation of the actin cytoskeleton. Daidzein significantly affected the candidate genes (FOSL1, DGKH, Gadd45G, GAL, SEMA3, TOB, FABP8, TRIB2, Nech1, and GSTA3) involved in adipocyte differentiation, as well as genes (CSTB and ACTN) related to connective tissue structure in heat-stressed Jinjiang cattle. Daidzein plays a positive role in relieving heat stress and improving beef quality in heat-stressed Jinjiang cattle. Full article

This study evaluated the microbiological quality, free amino acid profile (FAAs), and the biogenic amine (BA) accumulation in beef steaks during extended storage under 0–4 °C in modified atmosphere (MAP O₂/CO₂/N₂): MAP80/20/0, MAP70/20/10, MAP60/20/20, and vacuum (VP). The VP meat had significantly higher Enterobacteriaceae counts than MAP meat, influencing BA accumulation. The total plate count (TPC) exceeded the acceptable fresh meat limit (10⁷ cfu/g) on day 28 of beef storage, regardless of packaging method. The dynamics of the changes in the FAAs differed between VP and MAP beef throughout storage, which affected the BAs’ formation. From day 14 of storage, VP beef steaks had lower or significantly lower content of (p ≤ 0.05) FAAs such as histidine, lysine, ornithine, phenylalanine, tryptophan, and tyrosine, and significantly (p ≤ 0.05) higher content of BAs such as histamine, cadaverine, putrescine, 2-phenylethylamine, tryptamine and tyramine compared to MAP beef. Based on BAI values, VP beef was spoiled on day 14, which was two weeks earlier than MAP beef, demonstrating that vacuum packaging promotes faster BA accumulation, due to the growth of Enterobacteriaceae under low-oxygen conditions. MAP provided more stable microbiological quality and lower BAI values of beef during storage than VP. No differences in shelf life between various MAP gas mixtures were observed; however MAP 80/20/0 slowed down the formation of biogenic amines, which was reflected by lower BAI values. Full article

Feeding a balanced diet such as total mixed ration (TMR) is a widely adopted feeding strategy providing a uniformly blended diet of roughages, concentrates, and supplements that enhances ruminant productivity by optimizing nutrient utilization, stabilizing rumen fermentation, and improving microbial activity. Scientific studies have confirmed that TMR increases dry matter intake (DMI), milk yield, and growth performance in dairy and beef cattle, as well as in sheep and goats. TMR’s advantages include consistent feed quality, reduced selective feeding, and improved feed efficiency. A key benefit of TMR is its ability to promote the production of volatile fatty acids (VFAs), which are the primary energy source for ruminants, particularly propionate. This enhances energy metabolism, resulting in higher carcass yields, increased milk production, and economic benefits compared to conventional or supplementary feeding systems. However, TMR feeding is also susceptible to mycotoxin contamination (e.g., aflatoxins, zearalenone), potential effects on methane emissions, and the need for precise formulation to maintain consistency and optimise profitability. Prevention and good practices, including routine inspection of feed for pathogens and vulnerable ingredients, as well as careful management of particle size and forage-to-concentrate ratios, are crucial in preventing subacute ruminal acidosis (SARA) and the development of other subclinical diseases. Mycotoxin binders, such as hydrated sodium calcium aluminosilicate, can also reduce mycotoxin absorption. Another advantage of practicing TMR is that it can support sustainable farming by integrating agro-industrial byproducts, which minimises environmental impact. In conclusion, TMR is a widely adopted feeding strategy that significantly enhances ruminant productivity by optimizing nutrient utilization, stabilizing rumen fermentation, and improving microbial activity, leading to increased dry matter intake, milk yield, and growth performance. It offers key benefits such as consistent feed quality, reduced selective feeding, improved feed efficiency, and enhanced energy metabolism, providing economic advantages and supporting sustainable farming through agro-industrial byproduct integration. However, its implementation requires careful management to mitigate risks, including mycotoxin contamination, potential impacts on methane emissions, and digestive issues like SARA if formulation is not precise. Therefore, for sustainable production, future research should focus on optimizing TMR formulations with alternative ingredients (e.g., agro-industrial byproducts) and precision feeding strategies to enhance livestock health and animal productivity while minimizing environmental impacts. Full article

Bruises in beef cattle carcasses are important indicators related to pre-slaughter handling and transport conditions, with implications for animal welfare and meat quality. This study analysed 19.4 million cattle carcasses transported from 42,805 farms to 38 slaughterhouses in Brazil to identify factors associated with bruising. Logistic regression models were used to assess the effects of sex, age, transport distance from farm to industry, and truck class. At least one bruise was found in 33.8% of the analysed carcasses. Older animals had a greater prevalence of bruising, and females were the most affected. The relationship between transport distance and bruising varied across carcass regions, showing distinct patterns rather than a uniform trend. Compared with larger-capacity vehicles, smaller trucks increased the risk of bruising. The round, rump, and flank regions presented the greatest number of bruises. The models assessing individual effects demonstrated good overall performance, with accuracy ranging from 75% to 82% in identifying bruises. The best performance was observed for round-rump, likely due to the higher frequency of bruises in these cuts. These findings highlight the need to improve transport logistics, adopt better handling practices, and implement specific interventions to reduce bruising. Full article

Accurate and non-invasive assessment of fat and muscle composition is crucial for biomedical monitoring to track health conditions in humans and pets, as well as for classifying meats in the meat industry. This study introduces a cost-effective, multifunctional ultra-wideband microwave system operating from 2.4 to 4.4 GHz, designed for rapid and non-destructive quantification of fat thickness, muscle thickness, and fat-to-muscle ratio in diverse ex vivo samples, including pork, beef, and oil–water mixtures. The compact handheld device integrates essential RF components such as a frequency synthesizer, directional coupler, logarithmic power detector, and a dual-polarized Vivaldi antenna. Bluetooth telemetry enables seamless real-time data transmission to mobile- or PC-based platforms, with each measurement completed in a few seconds. To enhance signal quality, a two-stage denoising pipeline combining low-pass filtering and Savitzky–Golay smoothing was applied, effectively suppressing noise while preserving key spectral features. Using a random forest regression model trained on resonance frequency and signal-loss features, the system demonstrates high predictive performance even under limited sample conditions. Correlation coefficients for fat thickness, muscle thickness, and fat-to-muscle ratio consistently exceeded 0.90 across all sample types, while mean absolute errors remained below 3.5 mm. The highest prediction accuracy was achieved in homogeneous oil–water samples, whereas biologically complex tissues like pork and beef introduced greater variability, particularly in muscle-related measurements. The proposed microwave system is highlighted as a highly portable and time-efficient solution, with measurements completed within seconds. Its low cost, ability to analyze multiple tissue types using a single device, and non-invasive nature without the need for sample pre-treatment or anesthesia make it well suited for applications in agri-food quality control, point-of-care diagnostics, and broader biomedical fields. Full article

Yajiangxue cattle (XF) is three-way crossbred cattle developed specifically for producing high-quality beef in the Tibetan Plateau by introducing the bloods of Tibetan yellow cattle (HF) and Angus cattle into Tibetan yak (MF). In the present study, we mainly focused on fat deposition and metabolism changes and used RNA-seq and LC-MS/MS-based metabolomics to partially explain the meat quality improvement in Yajiangxue cattle. Differential expression analysis revealed 1762, 2949, and 2931 different expression genes in XF vs. HF, XF vs. MF, and XF vs. cattle–yak (PF), respectively, such as BMP2, WISP2, FGF1, IL1B, IL6, and WNT5B. Immune response, oxidation–reduction processes, and fatty acid metabolism were markedly enriched. Furthermore, an initial identification revealed 319 metabolites using positive ion mode and 289 metabolites using negative ion mode in bovine adipose tissue across four breeds/populations. Of these, 143 were differential metabolites in positive ion mode, while 166 were in negative ion mode. The main pathways of metabolism affected by breed/population were unsaturated fatty acid biosynthesis, tryptophan and tyrosine biosynthesis, primary bile acid biosynthesis, cholesterol metabolism, beta-alanine metabolism, etc. Similarly, both the transcriptome and the metabolome results highlighted fatty acid metabolism. These results could help elucidate the biological mechanisms involved in fat deposition and identify valuable biomarkers for specific metabolite accumulation. Full article

Microbial proteins offer a sustainable alternative for animal nutrition. Rossellomorea marisflavi NDS, a bacterium isolated from seawater, was previously identified as a promising candidate due to its high protein content. This study aimed to enhance its single cell protein production through systemic fermentation optimization. Single-factor optimization in shake flask determined the optimal conditions to be: a salinity of 20‰ NaCl, a temperature of 32 °C, and an initial pH of 7.3, and a medium composed of 1% (w/v) corn flour, 1% peptone, 0.3% beef extract, and 0.2% KCl. Scaling up to a 10 L bioreactor demonstrated that a two-stage agitation strategy (150 rpm for the first 20 h followed by 180 rpm for the remaining 12 h) enhanced single cell protein yield. Furthermore, allowing the pH to fluctuate freely was more beneficial for protein production than maintaining a constant pH of 7.3 ± 0.02. Under these optimized conditions, the biomass composition (wet weight) was determined to be 2.3767 ± 0.0205% crude ash, 15.6013 ± 0.0082% crude protein, 0.1023 ± 0.0026% crude lipid, and 2.6997 ± 0.0021% carbohydrates. Amino acid analysis revealed a rich profile, with lysine and glutamic acid being the predominant essential and non-essential amino acids, respectively. Fatty acids analysis indicated that C14:1n5 was the most dominant. These findings underscore the potential of R. marisflavi NDS as a high-quality dietary protein supplement and provide a solid foundation for its industrial-scale production. Full article

A prepared dish needs to be reheated before eating, and various reheating methods affect its flavor quality. This study evaluated the influence of stir-frying reheating, steaming reheating and microwave reheating on moisture content, lipid oxidation and flavor profiles of prepared beef with tangerine peel. Stir-frying reheating samples obtained a higher moisture content and the highest thiobarbituric acid reactive substance value. Fifty-seven volatile compounds were identified by gas chromatography–mass spectrometry, of which fifteen compounds were considered as odor-active compounds with an odor activity value > 1. Aldehydes were the most prominent contributors to the aroma of reheated samples. Results revealed that stir-frying reheating samples had the most varieties of odor-active compounds, and the odor activity values of most of them were relatively higher. The heatmap analysis based on the odor activity values indicated that the stir-frying reheating process could maintain the original flavor of samples. A total of fifty-two volatile organic compounds were identified by gas chromatography–ion mobility spectrometry, and the principal component analysis revealed that the three reheated samples could be well distinguished from each other. Moreover, the content of free amino acids and nucleotides in stir-frying reheating samples was higher than that in other reheated samples. In conclusion, different reheating treatments affected the flavor quality of beef samples, and stir-frying process was better to obtain the aroma and taste characteristics of samples. The results of this study could provide useful information about the appropriate reheating method of a dish of prepared beef with tangerine peel for consumers, caterers and industrial production. Full article