Search Results (967)

The Anderson localization of phonons in disordered superlattices has been proposed as a route to suppress thermal conductivity beyond the limits imposed by conventional scattering mechanisms. A commonly used signature of phonon localization is the emergence of the nonmonotonic dependence of thermal conductivity $\kappa$ on system length L, i.e., a $\kappa$-L maximum. However, such behavior has rarely been observed. In this work, we conduct extensive non-equilibrium molecular dynamics (NEMD) simulations, using the LAMMPS package, on both periodic superlattices (SLs) and aperiodic random multilayers (RMLs) constructed from Si/Ge and Lennard-Jones materials. By systematically varying acoustic contrast, interatomic bond strength, and average layer thickness, we examine the interplay between coherent and incoherent phonon transport in these systems. Our two-phonon model decomposition reveals that coherent phonons alone consistently exhibit a strong nonmonotonic $\kappa$-L. This localization signature is often masked by the diffusive, monotonically increasing contribution from incoherent phonons. We further extract the ballistic-limit mean free paths for both phonon types, and demonstrate that incoherent transport often dominates, thereby concealing localization effects. Our findings highlight the importance of decoupling coherent and incoherent phonon contributions in both simulations and experiments. This work provides new insights and design principles for achieving phonon Anderson localization in superlattice structures. Full article

The core objective of racehorse breeding is to enhance the speed and endurance of the horses. The Grassland-Thoroughbred is an emerging horse breed developed in northern China in recent years, characterized by excellent speed performance, enduring stamina, and strong environmental adaptability. However, research on the genetic characteristics within this breed and the genes associated with athletic performance remains relatively limited. We conducted whole-genome resequencing of Grassland-Thoroughbred F1, F2, F3, and the crossbred population (CY) and obtained a total of 4056.23 Gb of high-quality data after quality control. The single nucleotide polymorphisms (SNPs) were primarily distributed in intergenic regions, followed by intronic regions. Principal component analysis (PCA) and STRUCTURE revealed clear distinctions among the generations, with a notable overlap between CY and F3. Using the SNP dataset, we analyzed the number and length distribution patterns of runs of homozygosity (ROHs) in the genomes of different generational groups of Grassland-Thoroughbreds. Short ROHs ranging from 0.5 to 2 Mb were the most abundant, with the following distribution: F1 (85.15%) > F2 (82.92%) > CY (78.75%) > F3 (77.51%). Medium-length ROHs (2–8 Mb) and long ROHs (>8 Mb) together exhibited a similar but opposite trend. The average length of ROHs was 1.57 Mb. The inbreeding coefficients (F_ROH) among different generational groups of Grassland-Thoroughbreds were as follows: F1 (0.0942) < F2 (0.1197) < CY (0.1435) < F3 (0.1497). Through ROH island analysis, 10 high-frequency ROH regions were identified and annotated with 120 genes. Genomic regions and candidate genes associated with athletic traits—ACAD8, OPCML, PRDX2, NTM, NDUFB7, SCL25A15, FOXO1, and SLC4A10—were identified. These genes may play important roles in regulating muscle performance, mitochondrial energy supply, and learning and memory processes in horses and are closely associated with the athletic ability of the Grassland-Thoroughbred population. This study is the first to systematically characterize the genomic diversity and inbreeding dynamics of the Grassland-Thoroughbred during the breeding process. It identifies candidate genes that may influence athletic performance, thereby providing an important molecular foundation and theoretical basis for the genetic improvement and performance-based selection of this emerging breed. Full article

This study presents a microfluidic chip platform designed using a multiscale 3D printing strategy for fabricating microfluidic chips with integrated, high-resolution, and customizable membrane structures. By combining two-photon polymerization (2PP) for submicron membrane fabrication with liquid crystal display printing for rapid production of larger components, this approach addresses key challenges in membrane integration, including sealing reliability and the use of transparent materials. Compared to fully 2PP-based fabrication, the multiscale method achieved a 56-fold reduction in production time, reducing total fabrication time to approximately 7.2 h per chip and offering a highly efficient solution for integrating complex structures into fluidic chips. The fabricated chips demonstrated excellent mechanical integrity. Burst pressure testing showed that all samples withstood internal pressures averaging 1.27 ± 0.099 MPa, with some reaching up to 1.4 MPa. Flow testing from ~35 $\mathsf{\mu }$L/min to ~345 $\mathsf{\mu }$L/min confirmed stable operation in 75 $\mathsf{\mu }$m square channels, with no leakage and minimal flow resistance up to ~175 $\mathsf{\mu }$L/min without deviation from the predicted behavior in the 75 $\mathsf{\mu }$m. Membrane-integrated chips exhibited outlet flow asymmetries greater than 10%, indicating active fluid transfer across the membrane and highlighting flow-dependent permeability. Overall, this multiscale 3D printing approach offers a scalable and versatile solution for microfluidic device manufacturing. The method’s ability to integrate precise membrane structures enable advanced functionalities such as diffusion-driven particle sorting and molecular filtration, supporting a wide range of biomedical, environmental, and industrial lab-on-a-chip applications. Full article

The monolithic integration of III-V semiconductors with silicon (Si) is a critical step toward advancing optoelectronic and photonic devices. In this work, we present GaAs nanoheteroepitaxy (NHE) on Si nanotips using gas-source molecular beam epitaxy (GS-MBE). We discuss the selective growth of fully relaxed GaAs nanoislands on complementary metal oxide semiconductor (CMOS)-compatible Si(001) nanotip wafers. Nanotip wafers were fabricated using a state-of-the-art 0.13 μm SiGe Bipolar CMOS pilot line on 200 mm wafers. Our investigation focuses on understanding the influence of the growth conditions on the morphology, crystalline structure, and defect formation of the GaAs islands. The morphological, structural, and optical properties of the GaAs islands were characterized using scanning electron microscopy, high-resolution X-ray diffraction, and photoluminescence spectroscopy. For samples with less deposition, the GaAs islands exhibit a monomodal size distribution, with an average effective diameter ranging between 100 and 280 nm. These islands display four distinct facet orientations corresponding to the {001} planes. As the deposition increases, larger islands with multiple crystallographic facets emerge, accompanied by a transition from a monomodal to a bimodal growth mode. Single twinning is observed in all samples. However, with increasing deposition, not only a bimodal size distribution occurs, but also the volume fraction of the twinned material increases significantly. These findings shed light on the growth dynamics of nanoheteroepitaxial GaAs and contribute to ongoing efforts toward CMOS-compatible Si-based nanophotonic technologies. Full article

Mystus guttatus, a second-class protected species in China, has undergone severe population decline due to anthropogenic and environmental pressures, yet conservation efforts are hindered by limited genomic resources and a lack of mechanistic insights into its stress response systems. Here, the first full-length transcriptome of M. guttatus was generated via SMRT sequencing. A total of 32,647 full-length transcripts were obtained, with an average length of 1783 bp. After structure and function annotation of full-length transcripts, 30,977 genes, 1670 transcription factors (TF), 918 alternative splicing (AS), and 11,830 simple sequence repeats (SSR) were identified. In order to further explore the stress resistance of M. guttatus, 93 genes belonging to the heat shock protein (HSP) family were identified and categorized into HSP70 and HSP90 subgroups. After phylogenetic analysis and selective stress analysis, it was discovered that the hsp family has suffered purifying selection and gene loss, potentially contributing to a decrease in the stress resilience and population of M. guttatus. Using protein interaction network and molecular docking tools, we observed the intricate interplay among HSPs and discovered HSP70-HOP-HSP90 interaction, which is an essential stress response mechanism. Our study sequenced the first full-length transcriptome of M. guttatus to enhance its genomic resources for its conservation and breeding and provide new insights into the future study of stress response mechanisms on M. guttatus. Full article

Objectives: This study investigated the properties of a neutral exopolysaccharide (EPS-LP1) with an average molecular weight of 55,637 Da, isolated from Lactiplantibacillus plantarum DMDL 9010 (LP9010). Results: The composition of EPS-LP1 includes galactose (Gal), glucose (Glu) and mannose (Man) in a molar ratio of 5.35:86.25:8.40. Notably, EPS-LP1 exhibits a smooth and rod-like surface along with thermal stability. Methylation combined with nuclear magnetic resonance analysis revealed that EPS-LP1 structured as t-Galp(1→, →6)-Glcp(1→, 4)-Glcp(1→ and →4,6)-Galp(1→), with relative molar ratio of 1.016:9.874:4.355:78.693:6.062, respectively. In the concentration range of 50 to 400 mg/mL, we observed the absence of cytotoxic effects from EPS-LP1 on RAW264.7 cells. Furthermore, EPS-LP1 demonstrated protective effects on RAW264.7 cells against oxidative damage by reducing the production of reactive oxygen species (ROS), malondialdehyde (MDA), and lactate dehydrogenase (LDH) release. Conversely, an increase in superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and concentrations of glutathione (GSH) was observed. Immunoreactivity assays indicated that EPS-LP1 can effectively reduce the production of nitric oxide (NO) and inhibit the secretion of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Additionally, it inhibited the activation of the mitogen-activated protein kinase (MAPK)/nuclear factor-kappa B gene binding (NF-kB) signaling pathway. Conclusions: This research provides a foundation basis for further investigations into the neutral exopolysaccharide derived from LP9010. Full article

The walnut (Juglans regia L.) holds a significant position among Kazakhstan’s nut crops, both in terms of cultivation and consumption. Although genetic resources of walnut are accessible within the country, they remain underexplored, yet they represent a valuable foundation for breeding initiatives and the development of locally adapted varieties. Currently, a comprehensive genetic profile of these resources is lacking, which hampers their effective utilization. The aim of this research was to evaluate the genetic diversity within a collection of walnut accessions collected from the southern and southeastern regions of Kazakhstan, including Almaty and Turkestan regions. To achieve this, eight SSR molecular markers were employed, providing insights into the genetic structure and relationships among the germplasm. All markers exhibited a high degree of polymorphism, with the WGA276 locus standing out as the most informative, displaying the greatest number of alleles and a high Shannon diversity index. The average expected heterozygosity (He) was 0.704 and was significantly higher than the observed heterozygosity (Ho = 0.547), which was confirmed by t-test (t = −6.426, p < 0.05). The findings indicated substantial genetic variation and intra-population polymorphism: on average, 5.875 alleles per locus were identified, and the Shannon index was 1.444. The population from Turkestan region (population 1, Sairam-Ugam) demonstrated the highest levels of diversity. Analyses of genetic structure, conducted through STRUCTURE, PCoA, and UPGMA methods, confirmed the existence of two genetically distinct groups exhibiting considerable diversity. Future research should focus on conserving the highly diverse populations in the Turkestan region to facilitate the development of stress-tolerant varieties. These findings highlight the importance of conserving and harnessing the genetic resources of Kazakhstan’s J. regia populations for future breeding efforts. Full article

The dependence of conventional epoxy resins on fossil fuels and the environmental and health hazards associated with bisphenol A (BPA) demand the creation of sustainable alternatives. Because lignin is a natural resource and has an aromatic ring skeleton structure, it could be used as an alternative to fossil fuels. This study effectively resolved this challenge by utilizing a sustainable one-step epoxidation process to transform lignin into a bio-based epoxy resin. The results verified the successful synthesis of epoxidized bamboo lignin through systematic characterization employing Fourier transform infrared spectroscopy, hydrogen spectroscopy/two-dimensional heteronuclear single-quantum coherent nuclear magnetic resonance, quantitative phosphorus spectroscopy, and gel permeation chromatography. Lignin-based epoxy resins had an epoxy equivalent value of 350–400 g/mol and a weight-average molecular weight of 4853 g/mol. Studies on the curing kinetics revealed that polyetheramine (PEA-230) demonstrated the lowest apparent activation energy (46.2 kJ/mol), signifying its enhanced curing efficiency and potential for energy conservation. Mechanical testing indicated that the PEA-230 cured network demonstrated the maximum tensile strength (>25 MPa), whereas high-molecular-weight polyetheramine (PEA-2000) imparted enhanced elongation to the material. Lignin-based epoxy resins demonstrated superior heat stability. This study demonstrates the conversion of bamboo lignin into bio-based epoxy resins using a simple, environmentally friendly synthesis process, demonstrating the potential to reduce fossil resource use, efficiently use waste, develop sustainable thermosetting materials, and promote a circular bioeconomy. Full article

Biodegradable vascular stents (BVSs) face challenges related to inadequate mechanical strength, which can lead to adverse clinical outcomes. Improving the mechanical behavior of biodegradable vascular stents through structural design has been extensively explored. However, the corresponding effects of these mechanical enhancements on degradation characteristics remain under-investigated. The present work focuses on examining how different stent design strategies affect the mechanical behavior and degradation characteristics of poly (lactic acid) (PLA) stents. The commercial PLA stent DESolve was adopted, and nine modified stents were constructed based on the geometrical configuration of the DESolve stent. The mechanical properties of the modified stents during radial crimping and three-point bending simulations were thoroughly studied. The degradation dynamics of the stents were characterized by four indices (i.e., mean number average molecular weight, residual volume fraction, mean von Mises stress, and stent diameter). The results indicated that both the widening ratio and direction affected the mechanical performance of the stents by increasing the radial stiffness and radial strength, minimizing recoil%, and decreasing the bending flexibility. Although the widening direction had a relatively minor influence on stent degradation, the associated increase in material volume contributed to an improved volumetric integrity and enhanced lumen preservation. This study established a theoretical basis for evaluating both the mechanical and degradation behaviors of PLA stents, offering valuable insights for future structural design optimization. Full article

Sugars Will Eventually Be Exported Transporters (SWEETs) are involved in plant growth and development, particularly in resistance to adverse environments. Achnatherum splendens (Trin.) Nevski exhibits rhizosheath formation and demonstrates notable salt and drought tolerance. We identified 31 sugar transporter family genes (AsSWEETs) from the Achnatherum splendens genome in the NCBI database and performed bioinformatics analyses, including gene structure, subcellular localization, conserved sequences, promoter cis-acting elements, phylogenetic relationships, and chromosomal localization. The 31 AsSWEET genes are distributed across 13 chromosomes, encoding peptides ranging from 375 to 1353 amino acids. Their predicted molecular weights range from 31,499.38 to 109,286.91 Da, with isoelectric points (pI) between 4.78 and 5.21. The aliphatic index values range from 13.59 to 24.19, and the grand average of hydropathicity (GRAVY) values range from 0.663 to 1.664. An analysis of promoter cis-acting elements reveals that all 31 AsSWEET genes contain multiple elements related to light, stress, and hormone responses. Subcellular localization predictions indicate that most genes in this family are localized to the plasma membrane or tonoplast, with AsSWEET12-2 and AsSWEET3b localized in chloroplasts and AsSWEET2b-2 in the nucleus. qRT-PCR results show that AsSWEET13-1, AsSWEET13-3, and AsSWEET1a exhibit upregulated expression in response to salt and drought stress in the roots of Achnatherum splendens. These genes may serve as candidate genes for investigating the stress resistance mechanisms of Achnatherum splendens. The findings provide a theoretical basis for further research on stress resistance mechanisms and candidate gene identification under salt and drought stress in Achnatherum splendens. Full article

Sintered silver, widely used in WBG electronic device packaging for its excellent electrothermal properties and high-temperature stability, faces challenges in macroscopic mechanical behavior and reliability due to porosity, especially for pressureless sintered silver. However, the intrinsic pores inside sintered material introduce uncertainties during nanoindentation tests for mechanical characterization. This study investigated the impact of pore distribution on the dislocation behavior of pressureless sintered silver during nanoindentation. Firstly, pressureless sintered silver models with 8–33% porosity were prepared and characterized through scanning electron microscope (SEM) for porosity, electron backscatter diffraction (EBSD) for the geometrically necessary dislocation (GND) density distribution, and transmission electron microscopy (TEM) for the crystal structure and microscopic strain. The EBSD results indicated that nanoindentation caused localized plastic deformation in sintered silver, closely related to its porous structure. The TEM results revealed that sintered silver undergoes dislocation slip during nanoindentation, leading to complex dislocation network formation, while the strain decreased with distance from the indentation. To further investigate the relationship of pore distribution and dislocation behavior during nanoindentation, molecular dynamics (MD) simulations were carried out. The MD results revealed that the dislocation distribution was consistent with the EBSD and TEM results. During loading, with the increased porosity from 10% to 23.7%, the total dislocation length was reduced by 63%, while it led to a 38% increase in total dislocation length with the average pore size decreased from 3.84 nm to 2.88 nm under similar porosity conditions. This study improves the understanding of the deformation mechanisms of porous sintered silver under nanoindentation and provides insight into the mechanical characterization of porous materials. Full article

The formation of soil cracks in soil slopes can compromise structural integrity. Guar gum, as a natural high-molecular-weight biopolymer, offers environmental and economic advantages in soil stabilizers due to its biodegradability, strong binding properties, and ability to form a three-dimensional network structure. To investigate its improvement effects, outdoor dry shrinkage cracking tests were conducted on silt loam using different guar gum dosages. Image preprocessing was performed using Photoshop software, and Python algorithms combined with the PCAS system were employed to quantitatively analyze the development process of cracks, revealing the evolution patterns of basic crack parameters, fractal dimensions, and probability entropy. The results indicate the following: (1) the addition of guar gum improves the water retention capacity of the soil, with the average moisture content of the samples decreasing as the guar gum content increases; (2) as the guar gum content increased, the total length, total area, and surface crack ratio of the cracks all increased, but the average crack width decreased significantly, with the maximum decrease reaching 9.8%, indicating that guar gum can effectively suppress the expansion of crack width and slow down the infiltration rate of rainwater; (3) the fractal dimension of crack area is less affected by guar gum content, while the fractal dimension of crack length is significantly influenced by guar gum content. Combining both parameters can effectively characterize crack morphology and distribution. The final fractal dimension of crack length generally ranges from 1.2 to 1.3, while the fractal dimension of the crack area remains stable between 1.55 and 1.65; (4) the addition of guar gum has a minor effect on the probability entropy of cracks, with a change of less than 3%, indicating that it does not significantly influence the randomness of cracks. Therefore, this study confirms that guar gum has a significant effect in controlling crack width and optimizing the uniformity of the crack network. Through its mechanisms of binding soil particles and delaying drying shrinkage, it provides an important reference for the ecological protection of cohesive soil slopes. Full article

(1) Background: Ionizing radiation in the Earth’s atmosphere drives key chemical transformations affecting atmospheric composition. Despite their environmental relevance, experimental data on proton collisions with hydrofluorocarbons remain limited, and theoretical models for total cross-sections and stopping power are still underdeveloped. (2) Methods: This study applies Rudd’s semiempirical model to calculate proton impact ionization cross-sections for the CF₃CH₂F molecule, considering contributions from both outer and inner electron shells. The model enables the estimation of differential cross-sections and the average energy of secondary electrons. In addition, we calculate the photoionization cross-sections using a discretized representation of the continuum—the so-called pseudo-spectrum—obtained through TDDFT with PBE0 as an exchange–correlation functional and compare it with the cross-section obtained for proton impact in the high-energy limit. (3) Results: The Rudd model proves highly adaptable and suitable for numerical applications. However, its validation is hindered by the scarcity of experimental data. Existing models, such as SRIM and Bethe–Bloch, show significant discrepancies due to their limited applicability at intermediate energies and lack of molecular structure consideration. (4) Conclusions: A comparison between the Rudd and BEB models reveals strong agreement in the analyzed energy range. This consistency stems from both models accounting for the molecular structure of the target, as well as from the fact that protons and electrons possess charges of the same magnitude, supporting a coherent description of ionization processes at these energies. Full article

The glass transition temperature (T_g) is undoubtedly one of the most important characteristics of polymers, and investigating its dependence on their structure and composition is crucial from both fundamental and application points of view. This study deals with the unexpected relationship between T_g and the average molecular weight between crosslinking points (M_c) in nanophase-separated polystyrene-l-poly(dimethylsiloxane) (PSt-l-PDMS) and polystyrene-l-poly(dimethylsiloxane)/divinylbenzene (PSt-l-PDMS/DVB) polymer conetworks. In order to reveal the correlation between the T_g and M_c, a library of PSt-l-PDMS and PSt-l-PDMS/DVB conetworks was synthesized, and their compositions and T_gs were determined. Instead of the expected increase of T_g with decreasing M_c, a reverse correlation was found. Namely, the T_g decreases with decreasing M_c in these conetworks. Correlation analyses showed that the T_g linearly depends on 1/M_c, similar to the Fox–Flory relationship for homopolymers with their M_n, that is, T_g = T_g,ꝏ −K/M_c for the investigated conetworks, independent of the absence or presence of relatively low amounts of DVB as an additional small molecular weight crosslinker. This means that the PDMS macrocrosslinker acts like scissors by interrupting the mobility of the crosslinked PSt chains in the conetworks, and the T_g of the PSt segments will be close to that of PSt homopolymers with the same M_n as M_c, as found by comparison. Consistent with previous findings with other conetworks, the presence of the scissors effect of the macromolecular crosslinker in the PSt-l-PDMS and PSt-l-PDMS/DVB conetworks indicates that the scissors effect is a general phenomenon for polymer conetworks formed by crosslinking with a macromolecular crosslinker. The observed unusual T_g versus M_c relationship in the conetworks can be utilized in designing such novel materials with predetermined T_gs required for targeted applications. Full article

Immunotherapy is a transformative strategy in oncology, yet the development of novel immunomodulatory agents remains essential. This study explores the anti-tumor potential of a structurally unique polysaccharide isolated from an Aspergillus ochraceus (AOP), sourced from the Antarctic Weddell Sea. Using alkaline-assisted extraction and chromatographic purification, we obtained a homogeneous polysaccharide predominantly composed of galactose and mannose, with an average molecular weight of 39.67 kDa. The structure was characterized by an integrated nuclear magnetic resonance spectroscopy and mass spectrometry analysis, revealing that the AOP is composed of β (1→5)-linked galactofuranose units, with a minor substitution by α-D-mannopyranose residues via (1→2) glycosidic bonds at the C2 of the galactofuranose. Functional assays, including CCK8 and wound-healing tests, demonstrated that this polysaccharide, referred to as AOP, inhibited melanoma cell proliferation and migration in a dose-dependent manner. Additionally, the AOP activated RAW264.7 and bone marrow-derived macrophage (BMDM) cells without exhibiting significant cytotoxicity, leading to the release of inflammatory factors such as TNF-α, IL-1β, and IL-6. Mechanistically, the AOP was found to upregulate the expression of CD86 and IFN-γ, while downregulating genes like IL-4 and Arg1. These findings position the AOP as the first documented Antarctic fungal polysaccharide with macrophage-reprogramming capabilities against melanoma, offering novel molecular insights for marine-derived immunotherapeutics. Full article