Identification and Expression Pattern Analysis of AsSWEET Gene Family in Achnatherum splendens
Abstract
1. Introduction
2. Results
2.1. Identification and Physicochemical Characterization of the AsSWEET Gene Family
2.2. Secondary Structure Analysis of the AsSWEET Gene Family
2.3. Analysis of Transmembrane Domains in the AsSWEET Family Proteins
2.4. Analysis of AsSWEET Gene Structure and Protein Conserved Motifs
2.5. Phylogenetic Analysis of the AsSWEET Gene Family
2.6. Analysis of Cis-Acting Elements in the Promoter Region of the AsSWEETs Gene Family
2.7. Chromosomal Localization Analysis of the AsSWEETs Gene Family
2.8. Subcellular Localization Prediction Analysis of the AsSWEETs Gene Family
2.9. Expression Analysis of AsSWEETs Gene Family Members Under Abiotic Stress
2.10. Subcellular Localization
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Experimental Methods
4.2.1. Identification and Physicochemical Characterization of the SWEET Gene Family in A. splendens
4.2.2. Secondary Structure Analysis of the SWEET Gene Family in A. splendens
4.2.3. Transmembrane Domain Analysis of A. splendens SWEET Proteins
4.2.4. Gene Structure and Protein Motif Analysis of A. splendens SWEET Family
4.2.5. Phylogenetic Analysis of A. splendens SWEET Gene Family
4.2.6. Promoter cis-Acting Element Analysis of A. splendens SWEET Genes
4.2.7. Chromosomal Localization of A. splendens SWEET Genes
4.2.8. Subcellular Localization Prediction of A. splendens SWEET Gene Family Members
4.2.9. Abiotic Stress Expression Analysis of SWEET Gene Family Members in A. splendens
4.2.10. Construction of Plant Expression Vector
4.2.11. Subcellular Localization
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Name | Protein/ (aa) | MW(Da) | PI | Instability Index | Aliphatic Index | GRAVY |
---|---|---|---|---|---|---|
AsSWEET1a | 785 | 65,294.44 | 5.05 | 50.48 | 17.43 | 0.736 |
AsSWEET1a-1 | 801 | 66,709.30 | 5.04 | 54.29 | 16.73 | 0.728 |
AsSWEET1b | 732 | 61,692.02 | 5.08 | 48.68 | 15.85 | 0.829 |
AsSWEET2a-1 | 627 | 52,648.77 | 5.11 | 48.12 | 20.26 | 0.963 |
AsSWEET2a-2 | 816 | 69,883.04 | 5.07 | 43.95 | 18.38 | 0.915 |
AsSWEET2b-1 | 1353 | 109,286.91 | 4.99 | 41.24 | 18.99 | 0.839 |
AsSWEET2b-2 | 987 | 79,015.14 | 5.06 | 41.72 | 20.67 | 0.980 |
AsSWEET3b | 645 | 53,983.53 | 5.14 | 35.81 | 24.19 | 0.949 |
AsSWEET4 | 375 | 31,499.38 | 5.18 | 57.86 | 21.07 | 0.933 |
AsSWEET4-1 | 375 | 31,521.36 | 5.18 | 56.29 | 18.93 | 0.939 |
AsSWEET4-2 | 384 | 33,170.84 | 5.14 | 59.34 | 14.06 | 0.949 |
AsSWEET6a-1 | 669 | 56,383.84 | 5.08 | 54.43 | 19.73 | 0.817 |
AsSWEET6a-2 | 741 | 63,173.95 | 5.05 | 53.99 | 18.35 | 0.786 |
AsSWEET6a-3 | 690 | 58,671.54 | 5.07 | 53.71 | 19.28 | 0.79 |
AsSWEET11 | 975 | 83,415.84 | 4.98 | 52.94 | 16.10 | 0.849 |
AsSWEET11-1 | 909 | 77,606.83 | 5.00 | 55.62 | 16.28 | 0.837 |
AsSWEET11-2 | 777 | 66,672.56 | 5.02 | 56.25 | 14.67 | 0.871 |
AsSWEET12-1 | 804 | 68,695.44 | 5.03 | 58.07 | 17.91 | 0.799 |
AsSWEET12-2 | 687 | 57,957.26 | 5.08 | 52.75 | 17.61 | 0.860 |
AsSWEET13-1 | 675 | 55,438.35 | 5.08 | 51.67 | 20.59 | 1.004 |
AsSWEET13-2 | 831 | 69,651.03 | 5.03 | 52.59 | 16.49 | 0.814 |
AsSWEET13-3 | 657 | 55,396.52 | 5.08 | 52.79 | 16.89 | 0.892 |
AsSWEET14-1 | 858 | 71,819.04 | 5.01 | 55.83 | 18.65 | 0.853 |
AsSWEET14-2 | 1128 | 92,667.56 | 4.99 | 48.41 | 19.95 | 0.909 |
AsSWEET15-1 | 390 | 33,558.76 | 5.16 | 53.69 | 13.59 | 0.927 |
AsSWEET15-2 | 879 | 72,421.47 | 5.03 | 47.57 | 17.41 | 0.731 |
AsSWEET16 | 936 | 76,825.64 | 5.02 | 53.29 | 17.74 | 0.663 |
AsSWEET16-1 | 414 | 34,697.08 | 5.21 | 50.47 | 20.53 | 0.923 |
AsSWEET16-2 | 844 | 70,557.10 | 4.78 | 50.61 | 18.79 | 0.696 |
AsSWEET17-1 | 750 | 62,989.42 | 5.08 | 52.43 | 20.27 | 0.714 |
AsSWEET17-2 | 753 | 62,918.27 | 5.08 | 51.34 | 20.85 | 0.728 |
Gene Name | Alpha Helix (Hh) | Beta Turn (Tt) | Random Coil (Ee) | Extended Strand (Cc) |
---|---|---|---|---|
AsSWEET1a | 48.66 | 3.83 | 31.03 | 16.47 |
AsSWEET1a-1 | 55.26 | 3.01 | 26.69 | 16.36 |
AsSWEET1b | 47.33 | 4.12 | 29.22 | 19.05 |
AsSWEET2a-1 | 36.54 | 8.65 | 35.10 | 25.81 |
AsSWEET2a-2 | 41.61 | 10.22 | 20.44 | 21.83 |
AsSWEET2b-1 | 45.78 | 8.67 | 26.00 | 19.34 |
AsSWEET2b-2 | 36.59 | 6.71 | 34.45 | 27.74 |
AsSWEET3b | 41.59 | 3.27 | 38.79 | 23.98 |
AsSWEET4 | 42.74 | 3.23 | 30.65 | 22.26 |
AsSWEET4-1 | 43.55 | 4.03 | 26.61 | 23.18 |
AsSWEET4-2 | 51.18 | 7.87 | 21.26 | 23.39 |
AsSWEET6a-1 | 39.19 | 4.05 | 34.68 | 18.48 |
AsSWEET6a-2 | 39.84 | 4.47 | 31.71 | 16.48 |
AsSWEET6a-3 | 43.23 | 4.37 | 30.57 | 17.83 |
AsSWEET11 | 40.74 | 3.70 | 33.64 | 19.56 |
AsSWEET11-1 | 37.75 | 4.64 | 34.44 | 19.19 |
AsSWEET11-2 | 48.84 | 1.55 | 31.78 | 19.72 |
AsSWEET12-1 | 37.45 | 0.75 | 43.82 | 17.98 |
AsSWEET12-2 | 55.26 | 2.63 | 26.75 | 19.71 |
AsSWEET13-1 | 41.07 | 5.36 | 35.27 | 37.98 |
AsSWEET13-2 | 47.10 | 3.26 | 31.16 | 18.30 |
AsSWEET13-3 | 40.37 | 1.83 | 38.07 | 20.02 |
AsSWEET14-1 | 45.96 | 3.86 | 34.39 | 19.69 |
AsSWEET14-2 | 40.96 | 5.17 | 34.69 | 20.40 |
AsSWEET15-1 | 26.23 | 7.07 | 46.68 | 22.07 |
AsSWEET15-2 | 42.47 | 3.42 | 37.67 | 16.44 |
AsSWEET16 | 44.63 | 3.68 | 33.44 | 15.04 |
AsSWEET16-1 | 30.23 | 2.33 | 29.46 | 21.91 |
AsSWEET16-2 | 42.49 | 3.66 | 34.80 | 15.35 |
AsSWEET17-1 | 49.80 | 4.02 | 29.72 | 15.79 |
AsSWEET17-2 | 38.40 | 3.60 | 37.60 | 16.40 |
Gene Name | Subcellular Localization |
---|---|
AsSWEET1a | Plasma membrane |
AsSWEET1a-1 | Plasma membrane |
AsSWEET1b | Tonoplast |
AsSWEET2a-1 | Plasma membrane |
AsSWEET2a-2 | Tonoplast |
AsSWEET2b-1 | Plasma membrane |
AsSWEET2b-2 | Nucleus |
AsSWEET3b | Chloroplast |
AsSWEET4 | Extracellular matrix |
AsSWEET4-1 | Extracellular matrix |
AsSWEET4-2 | Tonoplast |
AsSWEET6a-1 | Plasma membrane |
AsSWEET6a-2 | Plasma membrane |
AsSWEET6a-3 | Plasma membrane |
AsSWEET11 | Plasma membrane |
AsSWEET11-1 | Plasma membrane |
AsSWEET11-2 | Plasma membrane |
AsSWEET12-1 | Plasma membrane |
AsSWEET12-2 | Chloroplast |
AsSWEET13-1 | Plasma membrane |
AsSWEET13-2 | Plasma membrane |
AsSWEET13-3 | Plasma membrane |
AsSWEET14-1 | Plasma membrane |
AsSWEET14-2 | Plasma membrane |
AsSWEET15-1 | Tonoplast |
AsSWEET15-2 | Plasma membrane |
AsSWEET16 | Plasma membrane |
AsSWEET16-1 | Tonoplast |
AsSWEET16-2 | Tonoplast |
AsSWEET17-1 | Tonoplast |
AsSWEET17-2 | Tonoplast |
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Hu, M.; Kou, W.; Chen, M.; Li, X.; Wang, J.; Niu, J.; Wang, F.; Li, H.; Li, R. Identification and Expression Pattern Analysis of AsSWEET Gene Family in Achnatherum splendens. Int. J. Mol. Sci. 2025, 26, 6438. https://doi.org/10.3390/ijms26136438
Hu M, Kou W, Chen M, Li X, Wang J, Niu J, Wang F, Li H, Li R. Identification and Expression Pattern Analysis of AsSWEET Gene Family in Achnatherum splendens. International Journal of Molecular Sciences. 2025; 26(13):6438. https://doi.org/10.3390/ijms26136438
Chicago/Turabian StyleHu, Ming, Wei Kou, Mingsu Chen, Xiaoying Li, Jingru Wang, Jiahuan Niu, Fei Wang, Hongbin Li, and Rong Li. 2025. "Identification and Expression Pattern Analysis of AsSWEET Gene Family in Achnatherum splendens" International Journal of Molecular Sciences 26, no. 13: 6438. https://doi.org/10.3390/ijms26136438
APA StyleHu, M., Kou, W., Chen, M., Li, X., Wang, J., Niu, J., Wang, F., Li, H., & Li, R. (2025). Identification and Expression Pattern Analysis of AsSWEET Gene Family in Achnatherum splendens. International Journal of Molecular Sciences, 26(13), 6438. https://doi.org/10.3390/ijms26136438