Search Results (91,499)

This study presents and evaluates three sampling strategies to characterize electricity demand in regions of Colombia with limited metering infrastructure. These areas lack Advanced Metering Infrastructure (AMI), relying instead on traditional monthly consumption records. The objective of the research is to obtain user samples that are representative of the original population and logistically efficient, in order to support energy planning and decision-making. The analysis draws on five years of historical data from 2020 to 2024. It includes monthly energy consumption, geographic coordinates, customer classification, and population type, covering over 500,000 users across four subregions of operation determined by the region grid operator: North, South, Center, and East. The proposed methodologies are based on Shannon entropy, consumption-based probabilistic sampling, and Kullback–Leibler divergence minimization. Each method is assessed for its ability to capture demand variability, ensure representativeness, and optimize field deployment. Representativeness is evaluated by comparing the differences in class proportions between the sample and the original population, complemented by the Pearson correlation coefficient between their distributions. Results indicate that entropy-based sampling excels in logistical simplicity and preserves categorical diversity, while KL divergence offers the best statistical fit to population characteristics. The findings demonstrate how combining information theory and statistical optimization enables flexible, scalable sampling solutions for demand characterization in under-instrumented electricity grids. Full article

Background and objective: Minimally invasive interventions, including irreversible electroporation (IRE), cryoablation, and high-intensity focused ultrasound (HIFU), offer promising alternatives for the treatment of low- and intermediate-risk prostate cancer. We aimed to evaluate the oncological efficacy and safety of these treatments. Methods: A systematic search of MEDLINE, Central, and EMBASE was conducted up to 5 January 2025, according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Recurrence, complication, survival, biochemical, and retreatment rates were evaluated, with risk of bias assessed using the Risk of Bias In Non-randomized Studies of Interventions (ROBINS-I) and Risk of Bias 2 (RoB2) tools. Results: 85 studies met the inclusion criteria, comprising 42 prospective cohort studies, 36 retrospective cohort studies, six registries, and one randomized controlled trial. Whole-gland HIFU showed significantly lower recurrence (15%) and postoperative mean PSA levels (0.68 ng/mL) than focal HIFU (24%, 2.81 ng/mL). Recurrence rates were similar for focal vs. extended IRE (30% vs. 26%) and focal vs. whole-gland cryoablation (18% vs. 13%). In-field and out-of-field recurrence rates were similar across treatment modalities (5–15%). Retreatment rates were low, with 6–7% of patients receiving a second ablation and 2–8% progressing to radical or hormonal therapy. Major complications were consistently rare. One-year biochemical recurrence-free survival (BRFS) exceeded 95%, and five-year BRFS approached 80% for HIFU and cryoablation. Conclusions: Minimally invasive focal and whole-gland therapies are safe and effective for treating low- and intermediate-risk prostate cancer, with high survival and low major complication rates. Notably, whole-gland HIFU achieves superior biochemical control and lower recurrence than focal HIFU, emphasizing the clinical importance of treatment extent. Full article

Aims: Prostate cancer (PCa) presents ongoing challenges in differentiating aggressive from indolent disease using traditional biomarkers such as prostate-specific antigen (PSA). The Phosphatase and Tensin Homolog (PTEN), a key tumour suppressor involved in cellular growth regulation, is emerging as a promising biomarker for risk stratification. This meta-analysis aims to evaluate the prognostic significance of PTEN loss in PCa, particularly its relationship with Gleason grade groups (GG), as defined by the ISUP system, and clinical outcomes. Methods: A systematic review and meta-analysis of 16 studies encompassing 11,375 patients was conducted in accordance with PRISMA guidance. Studies included evaluated PTEN loss, stratified by hemizygous and homozygous deletions, and its association with GG and clinical endpoints such as biochemical recurrence and lethal progression. Pooled odds ratios (ORs) and hazard ratios (HRs) were calculated using a random-effects model. Results: PTEN loss was significantly associated with tumour aggressiveness. Compared to GG1 tumours, the odds of PTEN loss were markedly increased in Gleason GG 2 and 3(OR: 2.78, 95% CI: 1.95–3.61) and GG ≥ 4 (OR: 6.35, 95% CI: 5.37–7.33). Homozygous PTEN deletions were more strongly associated with high-grade tumours than hemizygous deletions. Clinically, PTEN loss was predictive of adverse outcomes, including increased risk of biochemical recurrence (HR: 1.78, 95% CI: 1.31–2.25) and lethal progression (HR: 2.57, 95% CI: 1.12–3.95). Conclusion: PTEN loss correlates with higher GG and poorer clinical outcomes in PCa. Incorporating PTEN assessment into clinical decision making could improve risk stratification, guiding early intervention strategies and identifying patients suitable for active surveillance. Full article

Background/Objectives: Inherited platelet disorders (IPDs) are diverse conditions characterized by abnormalities in platelet count and function. Next-Generation Sequencing (NGS) shows promise as a diagnostic tool in the diagnosis of IPDs. This study aims to assess the clinical value and limitations of using a targeted NGS panel in diagnosing children with suspected IPDs. Methods: We conducted a retrospective study of 93 children evaluated for suspected IPDs. A targeted NGS panel of 14 IPD-associated genes (RUNX1, WAS, ADAMTS13, ANKRD26, CYCS, GATA1, GP1BA, GB1BB, GP9, ITGA2B, ITGB3, MASTL, MPL, MYH9) was performed. Results: Genetic variants were identified in 30 patients (32.3% of the cohort). A total of 37 variants, of which 15 (40.5%) were novel, were found across 11 of the 14 genes on the panel (all except MPL, CYCS, and RUNX1). Variants were most frequently found in ITGB3 (18.9% of variants), GP1BA (16.2%), and ADAMTS13 (16.2%) genes. The majority of variants (64.9%) were classified as variants of uncertain significance (VUS), followed by likely pathogenic (LP) (27%) and pathogenic (8.1%) variants. Most variants were in a heterozygous state (73%). Specific cases highlighted complex genetic scenarios, such as co-occurring variants, and the identification of pathogenic and LP variants in patients initially presenting with immune thrombocytopenia. Conclusions: NGS helps to identify genetic causes, assess risk, manage, and provide genetic counseling in the management of IPDs. However, the prevalence of VUS underscores the need for a multidisciplinary approach to evaluate NGS results accurately. Full article

This study investigates reading performances and eye movements in individuals with eccentric fixation due to age-related macular degeneration (AMD). Overall, 17 individuals with bilateral AMD (7 males; mean age 77.47 ± 5.96 years) and 17 controls (10 males; mean age 72.18 ± 5.98 years) were assessed for reading visual acuity (VA), reading speed (Minnesota low vision reading chart in Slovene, MNREAD-SI), and near contrast sensitivity (Pelli-Robson). Microperimetry (NIDEK MP-3) was used to evaluate preferential retinal locus (PRL) location and fixation stability. Eye movements were recorded with Tobii Pro-glasses 2 and analyzed for reading duration, saccade amplitude, peak velocity, number of saccades, saccade duration, and fixation duration. Individuals with AMD exhibited significantly reduced reading indices (worse reading VA (p < 0.001), slower reading (p < 0.001), and lower near contrast sensitivity (p < 0.001)). Eye movement analysis revealed prolonged reading duration, longer fixation duration, and an increased number of saccades in individuals with AMD per paragraph. The number of saccades per paragraph was significantly correlated with all measured reading indices. These findings provide insights into reading adaptations in AMD. Simultaneously, the proposed approach in analyzing eye movements puts forward eye trackers as a prospective diagnostic tool in ophthalmology. Full article

Background: Severe glenoid bone loss presents a major challenge in both primary and revision reverse shoulder arthroplasty (RSA). Standard implants often fail to achieve reliable fixation in these cases. Custom-made, 3D-printed glenoid components have emerged as a potential solution, offering anatomically tailored fit and fixation. This study evaluates the clinical and radiographic outcomes of custom-made glenoid implants in managing severe glenoid bone loss. Methods: A retrospective, multicenter study was conducted on 23 shoulders (11 primary and 12 revision RSAs) that received a custom-made glenoid component using the Enovis ProMade System (San Daniele del Friuli, Udine, Italy) between 2017 and 2022, with a minimum follow-up of 24 months. Preoperative planning utilized CT-based 3D modeling to design implants with patient-specific instrumentation. Clinical outcomes (ROM, pain, Constant–Murley score) and radiographic results were assessed. Statistical comparisons were made between primary and revision groups. Results: Both groups demonstrated significant improvements in shoulder mobility, pain relief, and Constant–Murley scores (all p < 0.001), with no significant differences between primary and revision groups in delta scores. Radiographically, no loosening was observed, with minimal radiolucent lines and low complication rates. Four cases of instability occurred, all in the revision group, with only one requiring conversion to hemiarthroplasty. No differences in radiographic outcomes were observed between groups. Conclusions: Custom-made glenoid implants provide a reliable solution for severe glenoid bone loss in both primary and revision RSA, yielding consistent functional improvement and implant stability. Further prospective studies with larger cohorts and long-term follow-up are warranted to confirm these findings and assess cost-effectiveness. Full article

The modernization of agriculture can help humanity address major challenges such as population growth, climate change, and labor shortages. Semi-autonomous agricultural robots offer clear advantages in automating tasks and improving efficiency. However, in open-field conditions, their autonomy is limited by the size and weight of onboard batteries. Wireless charging is a promising solution to overcome this limitation. This work proposes a methodology for the design, modeling, and experimental validation of a wireless power transfer (WPT) system for battery recharging of agricultural robots. A brief review of WPT technologies is provided, followed by key design considerations, co-simulation, and testing results. The proposed WPT system uses a resonant inductive power transfer topology with series–series (SS) compensation, a high-frequency inverter (85 kHz), and optimized spiral planar coils, enabling medium-range operation under agricultural conditions. The main contribution lies in the first experimental assessment of WPT performance under real agricultural environmental factors such as soil moisture and water presence, combined with electromagnetic safety evaluation and robust component selection for harsh conditions. Results highlight both the potential and limitations of this approach, demonstrating its feasibility and paving the way for future integration with intelligent alignment and adaptive control strategies. Full article

Thallium (Tl) is a non-essential and highly toxic heavy metal capable of replacing potassium (K⁺) in biological systems, leading to mitochondrial dysfunction, oxidative stress, and inhibition of protein synthesis. In humans, the estimated oral lethal dose ranges from 10 to 15 mg/kg, with acute mortality rates of 6–15% and chronic neurological sequelae in up to 55% of survivors. Environmental releases of thallium of up to 5000 metric tons annually from industrial and mining activities, combined with its high oral bioavailability and nonspecific multisystemic symptoms, underscore the urgent need for more effective therapeutic strategies. This review summarizes current evidence on Tl toxicity, including its mechanisms of action, clinical manifestations, and available treatments. It emphasizes the strategic selection of biological models: simple organisms such as Caenorhabditis elegans and Drosophila melanogaster enable high-throughput screening and early biomarker detection; zebrafish (Danio rerio) provide vertebrate-level evaluation of multi-organ effects; and rodent models offer systemic toxicokinetic and therapeutic validation. Human-derived organoids and induced pluripotent stem cell (iPSC) systems recreate tissue-specific microenvironments, allowing translational assessment of mitochondrial, neuronal, and cardiac toxicity. Integrating these models within a tiered and complementary framework, alongside environmental and clinical surveillance, can accelerate the development of targeted treatments and strengthen public health responses to Tl exposure. Full article

The recycling of polypropylene (PP) packaging films modified with biobased additives: biochar derived from the pyrolysis of natural fibers and diatomaceous earth was investigated. The aim was to assess the impact of these modifiers on the processing, rheological, mechanical, and thermal properties of the recycled material. The processing behavior was evaluated through extrusion with granulation to determine industrial applicability. Rheological properties, including viscosity and melt flow index (MFI), were measured to characterize flow behavior. Mechanical performance was assessed through tensile strength, hardness, three-point bending, and impact resistance tests. Thermal properties were analyzed using thermogravimetric analysis (TGA), Vicat softening temperature (VST), and differential scanning calorimetry (DSC). The results demonstrate that incorporating biochar and diatomaceous earth can modify and, in selected cases, enhance the processing and performance characteristics of recycled PP films, though their impact on thermal behavior is parameter-specific. While diatomaceous earth slightly increased the onset of thermal degradation (T₅), both fillers caused a slight decrease in the VST, indicating reduced heat resistance under load. Diatomaceous earth was found to effectively improve stiffness and impact strength, while biochar reduced viscosity and promoted finer crystalline structures. Both additives acted as nucleating agents, increasing crystallization temperatures, with diatomaceous earth additionally delaying thermal degradation onset. These findings highlight the potential of using sustainable, waste-derived additives in polymer recycling, supporting the development of environmentally responsible materials within circular economy frameworks. Full article

Background/Objectives: Computed tomography (CT)-based radiomic analysis is an emerging technique that enables non-invasive assessment of tumor characteristics. In gastrointestinal stromal tumors (GISTs), radiomics may reflect biological behavior such as proliferative activity, often indicated by Ki-67 expression. To our knowledge, this is the first systematic review and meta-analysis synthesizing evidence on the ability of CT radiomics to predict the Ki-67 index in GISTs, addressing an important gap in the literature. Methods: A systematic review and meta-analysis were conducted following PRISMA guidelines to evaluate the predictive performance of CT radiomics for Ki-67 expression in GISTs. A literature search of PubMed, Scopus, Science Direct, and the Cochrane Library was performed up to December 2024 using predefined terms. Extracted data included study design, patient demographics, imaging protocols, radiomic features, and diagnostic performance. Study quality was assessed using the QUADAS-2 tool. A random-effects meta-analysis summarized the pooled area under the ROC curve (AUC), sensitivity, and specificity. Subgroup and sensitivity analyses explored heterogeneity sources. Publication bias was assessed using Egger’s test and funnel plots. Results: Six studies involving 1632 patients were included. The pooled sensitivity and specificity for predicting Ki-67 expression were 0.71 and 0.76, respectively, with a summary AUC of 0.79. Subgroup analyses showed consistent results across different imaging protocols and radiomic feature sets, though the Ki-67 cutoff (8% vs. 10%) affected diagnostic performance. Moderate heterogeneity and potential publication bias in specificity were observed. Conclusion: CT-based radiomics demonstrates moderate accuracy for non-invasively predicting Ki-67 index in GISTs. While not a substitute for histology, it may support personalized preoperative planning and guide future immunotherapy strategies. In the future, radiomic signatures—particularly when integrated with molecular or immune-related biomarkers—could help refine patient selection and monitoring strategies for emerging therapies, including immunotherapy. Full article

Background: Exfoliation syndrome (XFS) is a form of deleterious ocular aging mediated by genetic and environmental factors that frequently produces glaucoma (XFG). We aimed to develop a genetic risk score (GRS), assess its clinical utility, and identify metabolites/metabolite classes associated with a high GRS. Methods: For 39,472 Nurses’ Health Studies (NHS, 1980–2018; NHS2, 1989–2019) and Health Professionals Follow-up Study (1986–2018) participants aged ≥ 40 years reporting eye exams and no baseline glaucoma, we formed an eight-single nucleotide polymorphism Genetic Risk Score (GRS8) using loci with genome-wide associations with XFS. We estimated relative risks (RR) for incident XFG suspect (XFGS)/XFG (n = 118 cases) and Harrell’s C statistics. Among 7547 participants with plasma metabolites measured via liquid chromatography-mass spectrometry, we evaluated the relation between GRS8 and 427 individual metabolites and 20 metabolite classes, adjusting for multiple comparisons. Results: Higher GRS8 was associated with XFGS/XFG (GRS8 RR_{Quintile(Q)5vs.Q1} = 3.82, 95% CI: 1.76, 8.29). GRS8 significantly (p = 0.04) improved model prediction from C-index of 88% (95% CI: 0.84, 0.92) to 93% (95% CI: 0.91, 0.95) when added to a basic risk model including age, sex, period at risk, intraocular pressure, and glaucoma family history. Metabolite class analyses revealed positive associations of bile acids and inverse associations of fatty acyls with GRS8 (adjusted p < 0.001). Conclusions: XFS GRS8 improved XFGS/XFG prediction, and a higher XFS GRS8 was associated with altered levels of fatty acyl and bile acid metabolite classes. Full article

Coastal marine ecosystems are facing serious ecological risks from metals pollution, threatening biodiversity and human health. The main objective of this study is to evaluate the spatial distributions and ecological risks of dissolved cadmium (Cd), lead (Pb), and copper (Cu) in the Shandong Peninsula coastal areas, China. Two sampling campaigns were conducted at 21 sites in early spring 2025 to measure the concentrations of the three trace metals in the study area using an electrochemical detection system. The results revealed higher metals concentrations in nearshore areas (e.g., port entrances, aquaculture zones, and estuaries). Specifically, the Cd, Pb, and Cu concentrations in the study area ranged from 0 to 0.079 µg L⁻¹, 0.30 to 0.84 µg L⁻¹, and 2.19 to 4.79 µg L⁻¹, with average concentrations of 0.033, 0.55, and 3.18 µg L⁻¹, respectively. The contamination factors (C_f) of the three metals were below 1, indicating low pollution levels and thus meeting China’s Class I seawater quality standard. However, the ecological risk assessment, employing complementary methods, revealed varying interpretations: the risk quotient (RQ), based on species sensitivity distribution and predicted no-effect concentrations (PNECs), indicated low risks associated with Cd and Pb (RQ < 0.1) but a high risk for Cu (RQ > 1) at all sites, attributable to the exceedance of Cu’s protective threshold (0.46 µg L⁻¹), despite its low C_f. These findings highlight the need for continuous monitoring of Cu due to its high ecological impacts. In contrast, the Hakanson potential ecological risk index (ERI), which incorporates toxicity coefficients, suggested overall low risks (ERI < 150) for the combined metals; however, Cd contributed approximately 70% to the ERI due to its high toxicity coefficient, warranting attention despite the low individual Erⁱ values for Cd across the study area. This study provides valuable recent data on metals pollution dynamics in the Shandong Peninsula coastal areas, offering a scientific basis for developing marine pollution control policies and sustainable marine resource management. Full article

This study conducts a detailed and systematic Life Cycle Assessment (LCA) of demineralized (DEMI) water production at the Al-Hilla Second Gas Power Plant in Iraq, employing the Open LCA-ReCiPe 8 Midpoint (H) method to evaluate potential environmental impacts across 18 midpoint categories. The analysis focuses on the production of 1 cubic meter of high-purity water, offering a comprehensive evaluation of the environmental burdens associated with chemical usage, energy consumption, and resource depletion. The results indicate that terrestrial ecotoxicity is the most dominant impact category (20.383 kg 1,4-DCB-eq), largely driven by the extensive use of treatment chemicals such as coagulants, disinfectants, and antiscalants. Climate change follows as the second highest impact category (3.496 kg CO₂-eq), primarily due to significant electricity consumption during energy-intensive stages, particularly reverse osmosis (RO) and electro-deionization (EDI). These stages also contribute notably to fossil resource depletion (1.097 kg oil-eq) and particulate matter formation, reflecting the heavy reliance on fossil fuel-based energy in the region. Additional environmental concerns identified include human toxicity (both carcinogenic and non-carcinogenic), freshwater and marine ecotoxicity, and metal/mineral resource depletion, all of which underscore the need for improved chemical and material management throughout the treatment process. While impacts from categories such as ozone layer depletion, ionizing radiation, and eutrophication are relatively low, their cumulative effect over time remains a concern for long-term sustainability. The energy assessment reveals that the RO and EDI units alone account for over 70% of the total energy consumption, estimated at 3.143 kWh/m³. This research provides insights into minimizing environmental burdens in water treatment systems, especially in regions facing energy and water stress. Full article

Background: The prevalence of dental caries has increased among children, largely due to nutritional habits or inadequate access to dental care. The present study aimed to investigate the prevalence of dental caries associated with various factors that lead to their appearance, such as food intake and body composition, among Romanian school-age children from two counties, Bistriţa Năsăud and Mureş. Methods: This cross-sectional study included 1100 children aged 6–10 years from two Romanian counties. Dental caries experience was assessed using the DMFT and dmft indices based on WHO criteria. Dietary intake and oral health behaviors were evaluated through a food frequency questionnaire completed by parents. Associations between dietary variables and dental caries were assessed using independent sample t-tests and Mann–Whitney U tests. A binary logistic regression model was used to estimate the likelihood of caries in the permanent dentition (DMFT > 0), with covariates including county, parental education, daily sugar intake, consumption of dairy products, body mass index (BMI), and waist-to-height ratio. Results: It was found that the children who daily consume cheese recorded a DMFT value lower than the children who did not consume (p < 0.05). Moreover, those who consume sugary foods recorded higher values of DMFT as compared with those who did not (p < 0.05). The body weight, BMI, and waist circumference are positively correlated with DMFT, but negatively with dmft (p < 0.05). The overall prevalence of caries was 79.8% in primary dentition and 63.6% in permanent dentition, with slightly higher rates observed in Bistriţa-Năsăud County compared to Mureş. It seems that the predictors of the caries’s presence among children include the mother’s education level, sugar intake frequency, and body fat ratio. Conclusions: Based on significant associations found, one can affirm that the dental caries of the Romanian school-age children is due to sugary food intake and body composition. In addition, the direct relationship between dental caries and childhood obesity showed through the correlation of BMI and dental health indices denotes that school-age children should reduce sugary foods and increase dairy products. Full article

Background/Objectives: Despite the bidirectional relationship between diabetes and periodontal disease, dental hygienists’ role in diabetes screening remains underexplored in Japan. Previous studies have not examined the relationship between attitudes and actual screening practices. This study aimed to assess dental hygienists’ attitudes regarding diabetes screening and medical–dental collaboration, examine current practices, and investigate the association between attitudes and practices. Methods: A nationwide cross-sectional survey was conducted among dental hygienists from 1340 dental clinics using stratified random sampling. Participants completed an anonymous questionnaire assessing attitudes (4-point Likert scale) and screening practices (4-point frequency scale). Exploratory factor analysis and multiple regression analysis examined attitude–practice associations. Results: Of 1340 surveys distributed, 95 valid responses were obtained (response rate: 7.2%). Participants showed low implementation rates for lifestyle and physical sign assessment in diabetes (below 35%, except thirst: 68.4%) but moderate-to-high rates for medical history evaluation (44.2–75.8%). Multiple regression analysis revealed that lack of confidence and knowledge in diabetes management was negatively associated with both lifestyle assessment (β = −0.38; 95% CI −0.72 to −0.23) and medical history evaluation (β = −0.55; 95% CI −0.63 to −0.32). Desire to participate in diabetes education was positively associated with medical history evaluation (β = 0.21; 95% CI 0.24 to 4.38). Conclusions: Despite low response rate limiting generalizability, this first nationwide Japanese survey indicates that confidence and knowledge deficits significantly hinder diabetes screening practices. Targeted educational interventions could enhance screening implementation and strengthen medical–dental collaboration, improving early diabetes detection. Full article