Search Results (18)

Atractylodes macrocephala (A.M.) is a traditional Chinese medicinal and edible herb renowned for its spleen-tonifying, dampness-resolving, diuretic, and antiperspirant properties. Its primary bioactive constituents are terpenoids, which have demonstrated anti-inflammatory, antitumor, and immunomodulatory activities. However, transcriptomic studies focusing on terpenoid biosynthesis in A.M. from different geographical origins remain limited. To investigate the molecular mechanisms underlying differential sesquiterpenoid production, we performed transcriptome sequencing on samples collected from four distinct regions in China. Sesquiterpenoid biosynthesis predominantly proceeds through the mevalonate (MVA) and methylerythritol phosphate (MEP) pathways. Comparative analysis revealed four key enzyme-encoding genes—HMGCR, ISPF, GCPE, and FDPS—whose differential expression patterns were further validated by quantitative real-time PCR (qRT-PCR). Samples from Shaanxi exhibited the highest upregulation of biosynthetic genes and the greatest enrichment of terpene-related metabolites, suggesting enhanced pharmacological potential. In contrast, samples from Fujian, Anhui, and Hebei displayed relatively lower activity, with only FDPS upregulated in the Hebei sample. High-performance liquid chromatography (HPLC) quantification confirmed regional differences in the levels of major terpenoids—including atractylodin, atractylenolide I, and atractylenolide III—which correlated well with the observed gene expression profiles. This study compared conspecific A.M. from different geographical regions and further revealed that the variation in terpenoid metabolites is closely related to environmental factors. These findings provide a theoretical basis for the further discovery of functional genes and offer important implications for the quality control of A.M. Full article

Nowadays deodorants and antiperspirants play an important role in maintaining daily hygiene, exerting a substantial influence on both physical comfort and social functioning. Consequently, they can be regarded as a pivotal component of contemporary personal hygiene programs. The aim of this study was to undertake a comparative analysis of the elemental composition of diverse samples (72) of various roll-on deodorants and antiperspirants, sticks, and solid natural potassium–aluminium alums. These analyses were performed using ICP-OES and SEM-EDS techniques. The obtained results were then subjected to statistical and chemometric analysis. Studies demonstrated that Al and Zr were the most significant elements in the tested samples. Aluminium, a prevalent component in antiperspirants, was quantified in concentrations ranging from 0.9% to 4.4%, and in potassium–aluminium alums up to 4.7%. Aluminium and zirconium compounds were found to be the predominant elements in stick antiperspirants, with zirconium levels reaching up to 3%. The presence of lead was quantified in 35 of the 72 samples, with 19 samples exhibiting concentrations exceeding 1 mg/L. The highest lead level, reaching 15.90 mg/L, was found in potassium–aluminium alum. Furthermore, SEM-EDS analysis was conducted to verify the elemental composition, to provide data on additional ingredients, and to partially verify the information contained on the product labels. Full article

Deodorants and antiperspirants available on the market are designed to reduce the discomfort associated with sweating. This study examined the types of active substances contained in deodorants and antiperspirants from international cosmetic brands available in Poland (part of the EU market) and the frequency of their use. Product compositions were analysed based on INCI (International Nomenclature of Cosmetic Ingredients) product labels. The investigation included the following 170 cosmetic products: 50 spray deodorants (from 50 different brands); 50 roll-on deodorants (from 50 brands); 20 stick deodorants (from 20 brands); 40 roll-on antiperspirants (from 40 brands); and 10 stick antiperspirants (from 10 brands). The most popular active components were Triethyl Citrate (51/120 formulations; 42.5%), followed by Alcohol (25.8%), Ethylhexylglycerin (25.0%), Caprylyl Glycol (12.5%), and Potassium Alum (10.0%). Antiperspirant products were dominated by aluminium-based compounds, with the most frequently used being the following aluminium-based salts: Aluminium Chlorohydrate (67.5%), Aluminium Sesquichlorohydrate (25.0%), and Aluminium Chloride (12.5%). In contrast, aluminium–zirconium complexes, such as Aluminum Zirconium Tri-, Penta-, and Octachlorohydrex Gly, were rarely used by cosmetic manufacturers. Additionally, composition complexity, i.e., the number of deodorizing and anti-sweating ingredients per single formulation, was examined for roll-on deodorants, stick deodorants, and roll-on antiperspirants. All tested antiperspirants and most deodorants contained fragrance-imparting ingredients; the most popular were Parfum/Fragrance, Limonene, Linalool, Citronellol, Citral, Benzyl Salicylate, Hexyl Cinnamal, and Geraniol. Full article

Cosmetic products contain numerous metals used as pigments, UV filters, preservatives, antiperspirants and antimicrobial agents, which are responsible for allergic skin reactions, with the most common being nickel. To reduce skin penetration of Ni, innovative pharmaceutical formulations such as lipogels with chelating action against the metal ions themselves can be used. Chelation therapy allows a chelating agent to combine with metal ions to form a stable ring structure called a chelate. The chelate structure is more soluble in water than the toxic metal, which facilitates removal of the toxic metal from the tissue and its excretion by the kidneys. The aim of the following work was to evaluate the chelating properties against nickel ions of different types of lipogels containing flavonoids such as resveratrol and epigallocatechin gallate with chelating activities largely dependent on the number and position of their hydroxyl groups. The results obtained showed that lipogels based on epigallocatechin gallate show high chelating action against nickel, especially at low concentrations. In addition, rheological studies showed an ideal profile to ensure viscoelasticity and swelling of the lipogel within 48 h, confirming reports of 75% epigallocatechin release from the lipogel after 48 h. Tests have shown that lipogels based on epigallocatechin gallate have high chelating action against nickel, especially at low concentrations. Full article

The etiopathogenesis of breast cancer depends on genetic conditions, but recently more attention has been paid to the dependence of BC on certain environmental factors, for example, metalloestrogens, which include aluminum (Al) contained in antiperspirants used daily. The use of Al derivatives in antiperspirants in concentrations specified by the FDA, as well as European regulations (SCCS, 2020), do not classify Al as a hazardous and carcinogenic substance for humans. However, Al used to treat excessive sweating raises concerns, as many in vitro studies indicate that it can cause gene instability, change gene expression or increase oxidative stress, and also affect the body’s hormonal balance as a metalloestrogen. The environmental reality is that the breast is constantly exposed to many different chemicals, such as Al. This article reviews the literature to determine whether Al-based products can harm the body, as there are many facts and myths on the subject. The aim of the study is to present the current state of knowledge on the use of aluminum antiperspirants and the risk of breast cancer (BC). The article is based on data from the scientific literature, published in the PubMed and Google Scholar databases, as well as Science Direct, Scopus, Ovid MEDLINE, Ovid EMbase. It includes articles published in the years 2003–2023 mainly in English. Literature databases regarding human and animal studies were searched. To sum up, evaluating the effect of Al as a risk factor for breast cancer requires many studies using different research models focused on long-term exposure to Al-containing antiperspirants. Consumers are advised to limit their exposure to Al by making a conscious choice to minimize exposure to this compound. Full article

Floating wheat is a classical herbal with potential efficacy in the treatment of hyperhidrosis. Aiming at revealing the main components and potential mechanisms of floating wheat, a comprehensive and unique phytopharmacology profile study was carried out. First, common wheat was used as a control to look for chemical markers of floating wheat. In the screening analysis, a total of 180 shared compounds were characterized in common wheat and floating wheat, respectively. The results showed that floating wheat and common wheat contain similar types of compounds. In addition, in non-targeted metabolomic analysis, when taking the contents of the constituents into account, it was found that there indeed existed quite a difference between floating wheat and common wheat and 17 potential biomarkers for floating wheat. Meanwhile, a total of seven components targeted for hyperhidrosis were screened out based on network pharmacology. Seven key differential components were screened, among which kaempferol, asiatic acid, sclareol, enoxolone, and secoisolariciresinol had higher degree values than the others. The analysis of interacting genes revealed three key genes, namely, MAP2K1, ESR1, and ESR2. The Kyoto Encyclopaedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses showed that various signaling pathways were involved. Prolactin signaling, thyroid cancer, endocrine resistance, gonadotropin secretion, and estrogen signaling pathways were the main pathways of the intervention of floating wheat in excessive sweating, which was associated with the estrogenic response, hormone receptor binding, androgen metabolism, apoptosis, cancer, and many other biological processes. Molecular docking showed that the screened key components could form good bindings with the target proteins through intermolecular forces. This study reveals the active ingredients and potential molecular mechanism of floating wheat in the treatment of hyperhidrosis and provides a reference for subsequent basic research. Full article

Hyperhidrosis is a condition characterized by excessive sweating that affects at least 5% of the world’s population. It is normally associated with unpleasant body odour which requires continuous maintenance throughout life. Antiperspirants and deodorants are the first-line treatments for hyperhidrosis. However, association of this class of product with the risk of other diseases has increased the search for an effective antiperspirant from natural sources. This study aimed to investigate the antibacterial and antiperspirant activities of Curcuma xanthorrhiza Roxb. Using an in vitro microbial assay against specific bacteria responsible for body odour and rat foot pad analysis. Quality control of the fractionated extract was conducted using reversed-phase HPLC based on two active markers: xanthorrhiza and curcumin. Toxicity tests were conducted based on the OECD guidelines (50–2000 mg/kg). Inhibition zone, MIC, and MBC values of the hexane fraction of C. xanthorrhiza showed equivalent antibacterial activity to positive control (p > 0.05). No swelling, redness, or rashes were observed at the injection area throughout the study. Treatment with 40, 80, and 160 mg/kg of the extract significantly decreased the sweat excretion up to 79.34% (p < 0.05) after 15 min compared to negative control. Histopathology analysis showed the reduced size of the secretory units in plantar sweat glands post treatment compared to negative control. In conclusion, the hexane fraction of C. xanthorrhiza is able to reduce sweat excretion in a rat model and inhibit the growth of bacteria causing body odour. Full article

This study investigates the effects of the antiperspirant aluminum chlorohydrate on the development of antibiotic resistance in commensal Staphylococcus epidermidis isolates. The isolates were exposed to aluminum chlorohydrate for 30 days. The bacteria that developed resistance to oxacillin and ciprofloxacin were isolated, and the expression levels of some antibiotic resistance genes were determined using quantitative reverse transcriptase PCR. Before and after exposure, the minimum inhibitory concentration (MIC) values of the bacteria were determined using the microdilution method. A time-dependent increase was observed in the number of bacteria that developed resistance and increased MIC values. Consistent with the ciprofloxacin resistance observed after exposure, an increase in norA, norB/C, gyrA, gyrB, parC, and parE gene expression was observed. In addition to aluminum chlorohydrate exposure, oxacillin resistance was observed in all test bacteria in the group only subcultured in the medium, suggesting that phenotypic resistance cannot be correlated with chemical exposure in light of these data. The increase in mecA gene expression in selected test bacteria that acquired resistance to oxacillin after exposure compared with control groups suggests that the observed resistance may have been related to aluminum chlorohydrate exposure. To our knowledge, this is the first time in the literature that the effects of aluminum chlorohydrate as an antiperspirant on the development of antibiotic resistance in Staphylococcus epidermidis have been reported. Full article

Synthetic peptides, including tetraspanin CD9 peptides, are increasingly coming into focus as new treatment strategies against various organisms, including bacteria, that cause underarm odour. The use of deodorants and antiperspirants is associated with side effects. Therefore, it is critical to find an alternative therapeutic approach to combat underarm odour. The aim of this study is to investigate the antibacterial effect of tetraspanin CD9 peptides against the skin microbiota that cause malodour in the underarms. The antimicrobial activity of CD9 peptides against Micrococcus luteus (M. luteus), Bacillus subtilis (B. subtilis), Staphylococcus epidermidis (S. epidermidis), and Corynebacterium xerosis (C. xerosis) was investigated by the disc diffusion method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by broth microdilution assays using CD9 peptide concentrations ranging from 1 mg/mL to 0.0078 mg/mL. In addition, the anti-biofilm activity of the CD9 peptides was determined. The CD9 peptides showed different antibacterial activity with an inhibition zone of 7.67, 9.67, 7.00, and 6.00 mm for S. epidermidis, M. luteus, C. xerosis, and B. subtilis, respectively. All bacteria had the same MBC value of 1 mg/mL. A high MIC of CD9 peptides was observed for S. epidermidis and M. luteus at 0.5 mg/mL. The MIC values of B. subtilis and C. xerosis were 0.125 mg/mL and 0.25 mg/mL, respectively. CD9 peptides significantly inhibited biofilm development of S. epidermidis, B. subtilis, and C. xerosis isolates. The CD9 tetraspanin peptide has excellent antibacterial activity against bacteria that cause underarm odour. Therefore, the CD9 tetraspanin peptide is a promising alternative to deodorants and antiperspirants to combat commensal bacteria of the skin that cause underarm odour. Full article

Interest in developing selective and sensitive metal sensors for environmental, biological, and industrial applications is mounting. The goal of this work was to develop a sensitive and selective sensor for certain metal ions in solution. The goal was achieved via (i) preparing the sensor ((E)-2-((pyridine-3-ylimino)methyl)phenol) (3APS) using microwave radiation in a short time and high yield and (ii) performing spectrophotometric titrations for 3APS with several metal ions. 3APS, a Schiff base, was prepared in 5 min and in a high yield (95%) using microwave-assisted synthesis. The compound was characterized by FTIR, XRD, NMR, and elemental analysis. Spectrophotometric titration of 3APS was performed with Al(III), Ba(II), Cd(II), Co(II), Cu(II), Fe(III), Mn(II), Ni(II), and Zn(II). 3APS showed good abilities to detect Al(III) and Fe(III) ions fluorescently and Cu(II) ion colorimetrically. The L/M stoichiometric ratio was 2:1 for Cu(II) and 1:1 for Al(III) and Fe(III). Low detection limits (μg/L) of 324, 20, and 45 were achieved for Cu(II), Al(III), and Fe(III), respectively. The detection of aluminum was also demonstrated in antiperspirant deodorants, test strips, and applications in secret writing. 3APS showed high fluorescent selectivity for Al(III) and Fe(III) and colorimetric selectivity towards Cu(II) with detection limits lower than corresponding safe drinking water guidelines. Full article

Aluminum is an element found in nature and in cosmetic products. It can interfere with the metabolism of other cations, thus inducing gastrointestinal disorder. In cosmetics, aluminum is used in antiperspirants, lipsticks, and toothpastes. The aim of this work is to investigate aluminum bioavailability after accidental oral ingestion derived from the use of a toothpaste containing a greater amount of aluminum hydroxide than advised by the Scientific Committee on Consumer Safety (SCCS). To simulate in vitro toothpaste accidental ingestion, the INFOGEST model was employed, and the amount of aluminum was measured through the ICP-AES analysis. Tissue barrier integrity was analyzed by measuring transepithelial electric resistance, and the tissue architecture was checked through light microscopy. The margin of safety was also calculated. Overall, our results indicate that the acute exposure to aluminum accidentally ingested from toothpastes is safe for the final user, even in amounts higher than SCCS indications. Full article

Hyperhidrosis, the medical term for excessive sweating beyond physiological need, is a condition with serious emotional and social consequences for affected patients. Symptoms usually appear in focal areas such as the feet, hands, axillae and face. Non-surgical treatment options such as topical antiperspirants or systemic medications are usually offered as a first step of treatment, although these therapies are often ineffective, especially in severe and intolerable cases of hyperhidrosis. In the treatment algorithm for patients suffering from hyperhidrosis, surgical thoracoscopic sympathicotomy offers a permanent solution, which is particularly effective in the treatment of palmar hyperhidrosis and facial blushing. In this review, we describe the current status of thoracoscopic sympathicotomy for palmar hyperhidrosis and facial blushing. In addition, we share the specific treatment approach, technique and results of our Hyperhidrosis Expert Center. Last, we share recommendations to ensure an effective, reproducible and safe application of single-port thoracoscopic sympathicotomy for palmar hyperhidrosis and facial blushing, based on our extensive experience. Full article

Atomization is an intricate operation involving unstable and complex networks with rupture and fusion of liquid molecules. There are diverse details that typify the spray formation, which are the technique and configuration of the atomization process, dimension and structure of the nozzle, experimental parameters, etc. Ultimately, the process generates fine sprays from the bulk of a liquid. Some examples of atomization that we come across in our day-to-day life are antiperspirant or hair spray, shower head, garden sprinkler, or cologne mist. In this review paper we are briefly discussing the theoretical steps taking place in an atomization technique. The instabilities of the jet and sheet are explained to understand the underlying theory that breaks the jet or sheet into droplets. Different types of atomization processes based on the energy sources are also summarized to give an idea about the advantages and disadvantages of these techniques. We are also discussing the various biomedical applications of the electrohydrodynamic atomization and its potential to use as a drug delivery system. In short, this paper is trying to demonstrate the diverse applications of atomization to show its potency as a user friendly and cost-effective technique for various purposes. Full article

Astragalus L. is the largest genus in the family Leguminosae with over 3000 species and is represented in Turkey by 445 species, of which 224 are endemic. Several Astragalus species have many biological properties such as hepatoprotective, immunostimulant, antiviral, anti-aging, antitumor, antioxidant, antimicrobial, antimalarial, antileshmanial, antiperspirant, anti-inflammatory, diuretic and tonic. The aim of this research was the investigation of the cytotoxic activities of extracts obtained from underground and aerial parts of Astragalus argaeus Boiss. on a human breast cancer cell line (MCF-7). The cytotoxic effects of extracts on MCF-7 (human breast cancer cell lines) and fibroblast cells during 24 and 48 h were determined by 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphynyl tetrazolium bromide (MTT) assay. The extracts showed weak cytotoxic activity on MCF-7 during 24 h. To the best of our knowledge, this study is the first report on cytotoxic activity of A. argaeus. Full article

Background: Blisters are the most common dermatologic problem in ultraendurance runners. Their incidence, localization, pain scores, and risk factors in field conditions are poorly understood. Methods: We conducted an observational field-based cohort study during the 5-day multistage 2010 and 2011 Al Andalus Ultimate Trail (219 km). Daily postrace data on blister frequency, localization, severity, and preventive measures from 50 ultramarathon runners were collected through the direct interview technique. Results: After 4 days of running (182 km), blisters occurred in 76% of the participants (P < .001 versus stage 1) compared with 34% after day 1, 54% after day 2, and 72% after day 3 (P < .001 versus stage 1). Most of the blisters formed on the toes (65%) (P < .001), followed by blisters on other locations of the foot: the ball of the foot (16%), heel (14%), and sole (5%). Blisters were more painful toward the end of the race, and those on the sole and heel tended to be the most painful, although this did not reach statistical significance. Prophylactic measures studied (type and fabric of socks; application of antiperspirants, talcum powder, or lubricant to feet; and prophylactic taping) did not show any reduction in blister rates. The only predictive marker for reduced blister incidence was previous ultramarathon experience in men (r = −0.44, P < .05). Conclusions: Blisters are extremely common in multistage ultramarathon races. Race experience in male ultramarathon runners is associated with reduced blister rates. (J Am Podiatr Med Assoc 104(5): 473-478, 2014) Full article