Search Results (166)

Background: The B3-domain transcription factor ABI3 (ABSCISIC ACID INSENSITIVE 3) is a critical regulator of seed maturation, stress adaptation, and hormonal signaling in plants. However, its evolutionary dynamics and functional roles in cotton (Gossypium spp.) remain poorly characterized. Methods: We conducted a comprehensive genome-wide investigation of the ABI3 gene family across 26 plant species, with a focus on 8 Gossypium species. Analyses included phylogenetics, chromosomal localization, synteny assessment, gene duplication patterns, protein domain characterization, promoter cis-regulatory element identification, and tissue-specific/spatiotemporal expression profiling under different organizations of Gossypium hirsutum. Results: Phylogenetic and chromosomal analyses revealed conserved ABI3 evolutionary patterns between monocots and dicots, alongside lineage-specific expansion events within Gossypium spp. Syntenic relationships and duplication analysis in G. hirsutum (upland cotton) indicated retention of ancestral synteny blocks and functional diversification driven predominantly by segmental duplication. Structural characterization confirmed the presence of conserved B3 domains in all G. hirsutum ABI3 homologs. Promoter analysis identified key stress-responsive cis-elements, including ABA-responsive (ABRE), drought-responsive (MYB), and low-temperature-responsive (LTRE) motifs, suggesting a role in abiotic stress regulation. Expression profiling demonstrated significant tissue-specific transcriptional activity across roots, stems, leaves, and fiber developmental stages. Conclusions: This study addresses a significant knowledge gap by elucidating the evolution, structure, and stress-responsive expression profiles of the ABI3 gene family in cotton. It establishes a foundational framework for future functional validation and targeted genetic engineering strategies aimed at developing stress-resilient cotton cultivars with enhanced fiber quality. Full article

This article critically considers sample multigenerational oral traditions of Numic-speaking communities known as the Nüümü (Northern Paiute), Nuwu (Southern Paiute), and Newe (Western Shoshone), written down over the last 151 years. Utilizing the GOAT! phenomenological method to compare the onto-epistemologies of Numic peoples with a wide range of data from (G)eology, (O)ral traditions, (A)rchaeology and (A)nthropology, and (T)raditional knowledge, the author analyzed 824 multigenerational ancestral teachings. These descriptions encode multigenerational memories of potential geological, climatic, and ecological observations and interpretations of multiple locations and earth processes throughout the Numic Aboriginal homelands within California and the Great Basin. Through this layered and comparative analysis, the author identified potential convergences of oral traditions, ethnography, ethnohistory, rock art, and geological processes in the regions of California, the Great Basin, and the Colorado Plateau, indicative of large-scale earth changes, cognized by Numic Indigenous communities as earth birthing events, occurring during the Late Pleistocene/Early Holocene to Middle and Late Holocene, including the Late Dry Period, Medieval Climatic Anomaly, and Little Ice Age. Full article

This study analyzes forest fire legislation and policies in the Andean Community of Nations (ACN)—Colombia, Ecuador, Peru, and Bolivia—focusing on prevention and control. Using a comparative law approach, similarities, differences, and implementation challenges were identified. Ecuador and Peru have more comprehensive legal structures, while Colombia’s is simpler, and Bolivia falls in between. To address these gaps, this study proposes an Andean Directive for Integrated Fire Management (ADIFM) to harmonize policies and incorporate fire ecology, ancestral knowledge, education, monitoring technologies, and post-fire restoration. This regulatory framework, tailored to Andean ecological and sociocultural conditions, would optimize fire management and strengthen ecosystem resilience. Additionally, harmonizing sanctions and regulations at the regional level would ensure more coherent and effective governance. The ADIFM would provide strategic guidance for policymakers, fostering sustainable fire management and environmental restoration across Andean ecosystems. Full article

This study explores the application of three fermented plant materials—wheat, acorns, and sorghum—in couscous preparation, as well as their impact on its properties. A survey was conducted in some localities in Algeria. The aim is to reproduce the diagrams for the manufacture of different types of couscous incorporated with fermented materials and to evaluate the pasting properties, culinary qualities, and microstructure of each type of couscous produced. The survey identified four couscous formulations made with durum wheat semolina: couscous 1 (4% sorghum, 4% wheat, 8% acorns), couscous 2 (8% acorns), couscous 3 (0.8% sorghum, 6% acorns), and couscous 4 (4% wheat, 4% acorns). A comparative study of the four types of couscous showed significant differences in their physicochemical and microstructural properties. Formulations C3 and C4 showed the best functional performance among all the couscous samples studied. In terms of the swelling index, measured at 25 °C and 95 °C, C3 reached 131.11% and 165.55%, respectively, while C4 recorded 124.9% and 157.0%. Furthermore, these two formulations had the highest viscosity values: initial viscosity of 25 mPas (C3) and 27 mPas (C4), maximum viscosity of 31 mPas (C3) and 30 mPas (C4), and final viscosity of 49 mPas (C3) and 46 mPas (C4). Analysis of the cooking loss revealed higher values for couscous 1 and 2. The microstructure of couscous 2 revealed the presence of native starch particles, open porosity, and a state of partial gelatinization. The study revealed that formulations C3 and C4 significantly (p < 0.05) impact couscous structure by enhancing functionality while preserving quality. It also maintained ancestral knowledge and offered valuable insights for future industrial applications. Full article

This study offers a unique perspective on the role of ancestral knowledge in sustainable river management and community resilience. Specifically, this study draws on (1) a systematic literature review using the PRISMA methodology and (2) a qualitative analysis of community surveys conducted with 39 women in Zambrano, Colombia, to examine the impact of ancestral knowledge on sustainability, peace promotion, and community development. The findings highlight that women’s traditional water management practices significantly contribute to environmental sustainability, conflict resolution, and social cohesion. Women play a central role in transmitting and applying ancestral water knowledge, yet they remain marginalized in decision-making processes, often facing barriers to participation in governance structures. Finally, these findings proposed strategies for integrating ancestral knowledge into sustainable resource management policies. This study underscores the urgent need for legal recognition, intercultural dialogue, gender-inclusive governance, and educational programs to ensure the transmission and adaptation of these practices in contemporary contexts. Integrating ancestral knowledge into water management policies is essential for strengthening gender equity, community resilience, and fostering governance models that harmoniously combine traditional and scientific approaches. Full article

While ethnography has held an essential place in the study of Indigenous rock imagery (i.e., petroglyphs and pictographs) in the United States for the past century and a half, rarely are Tribes and other descendant communities involved throughout the entire research program—from conception to publication. This contrasts with recent developments within more traditional “dirt” archaeology, where over the past 30 years, Tribes have assumed greater roles in decision-making, fieldwork, artifact curation, data management, interpretation of results, and repatriation of ancestral belongings. In concert with these changes, Indigenous archaeology has emerged as a domain of theory and practice wherein archaeological research and cultural heritage management center the voices and interests of Indigenous communities. Collaboration among researchers and Indigenous communities has proven to be an effective means of practicing Indigenous archaeology and advancing its goals, but research into rock imagery all too often still limits Indigenous engagement and knowledge to the interpretation of the imagery. This article highlights a case study in Tribal collaboration from the North American Southwest in the interest of advancing an Indigenous archaeology of rock imagery. Full article

In the face of the growing challenges of the Anthropocene—marked by climate change, biodiversity loss, and increasing rates of disease and despair—this paper explores the need for holistic solutions that integrate cultural and spiritual transformation as essential dimensions of change. Recognizing that the interconnected challenges to planetary health stem from destructive socio-political agendas and unhealthy economic structures, we underscore the importance of worldviews and value systems as root causes of social and ecological injustices. Solutions require an understanding of the complex interdependence of systems, fostering mutualistic mindsets, and healing the ‘relationship crisis’ between humans and the natural world by cultivating a deeper level of consciousness. In response to these urgent needs, we describe Earthrise—a community of engaged contemplative practice led by the Nova Institute for Health in collaboration with the Planetary Health Alliance—dedicated to spiritual and cultural transformation in the face of today’s complex crises. Through intentional spiritual relationships—with ourselves, each other, and the natural world—our community emphasizes the power of narrative co-creation in building social cohesion and collective action for environmental stewardship. Our work is not solely contemplative, but also relational and integrative—embodying values through lived practice, community, and ecological engagement. Our activities focus on developing cultural capacities and self-awareness as essential foundations for fair and sustainable social transformation. By integrating diverse perspectives, including ancestral wisdom and Indigenous knowledge systems, we enrich worldviews and deepen our connection to the planet. The Earthrise community seeks to cultivate a sense of belonging, nurturing the meaningful relationships that foster compassion and care. Central to our approach is the use of creative emergence, leveraging the arts to inspire change and catalyze new paradigms. Through this exploration of interconnected themes, we contend that spiritual and cultural transformation is vital to advancing a thriving future, where human flourishing and planetary health are understood as inseparable and interconnected goals. Full article

This research investigates how historical architectural practices offer valuable solutions for contemporary climate adaptation challenges. Through systematic documentary analysis, we examine how European builders across centuries developed sophisticated construction techniques to address climate variability—techniques that remain relevant as we face increasingly extreme climate conditions. Our study focuses mainly on La Aljafería Palace in Zaragoza, Spain, a remarkable 11th-century Islamic structure that exemplifies bioclimatic design principles. We analyze its ingenious architectural elements—strategic courtyards, thermal mass management, passive ventilation systems, and innovative water features—that collectively create comfortable interior environments despite the region’s harsh summer climate. Similar analyses were conducted on historical structures in Italy, Greece, Portugal, and Cyprus as part of the ClimAid European project. Our findings reveal that these ancestral building practices utilized locally available materials and passive design strategies that required minimal energy inputs while providing effective climate regulation. We conclude that modern architects, conservationists, and policymakers face a dual challenge: developing strategies to reduce the vulnerability of historical structures to current climate impacts while also learning from and adapting these time-tested techniques to contemporary sustainable design. This research demonstrates how cultural heritage can serve not merely as an object of preservation but as a valuable knowledge repository for addressing present-day environmental challenges. Full article

The ability of a nucleic acid molecule to self-replicate is the driving force behind the evolution of cellular life and the transition from RNA to DNA as the genetic material. Thus, the physicochemical properties of genome replication, such as the requirement for a terminal hydroxyl group for de novo DNA synthesis, are conserved in all three domains of life: eukaryotes, bacteria, and archaea. Canonical DNA replication is initiated from specific chromosomal sequences termed origins. Early bacterial models of DNA replication proposed origins as regulatory points for spatiotemporal control, with replication factors acting on a single origin on the chromosome. In eukaryotes and archaea, however, replication initiation usually involves multiple origins, with complex spatiotemporal regulation in the former. An alternative replication initiation mechanism, recombination-dependent replication, is observed in every cellular domain (and viruses); DNA synthesis is initiated instead from the 3′ end of a recombination intermediate. In the domain archaea, species including Haloferax volcanii are not only capable of initiating DNA replication without origins but grow faster without them. This raises questions about the necessity and nature of origins. Why have archaea retained such an alternative DNA replication initiation mechanism? Might recombination-dependent replication be the ancestral mode of DNA synthesis that was used during evolution from the primordial RNA world? This review provides a historical overview of major advancements in the study of DNA replication, followed by a comparative analysis of replication initiation systems in the three domains of life. Our current knowledge of origin-dependent and recombination-dependent DNA replication in archaea is summarised. Full article

Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), remains a global health challenge. The human-adapted TB-causing bacteria are distributed into ten lineages with distinct global distributions and clinical outcomes. Mtb lineages 4 (L4) and L6 are good prototypes of these differences, because L4 is globally prevalent, whereas L6 is geographically restricted to West Africa and associated with slower disease progression. Given the fundamental role of T cells for the control of TB, we questioned whether Mtb L4 or L6 antigens and HLA interactions would be disrupted in West African hosts. Here, we selected variable and validated antigens and demonstrate their expression during in vivo Mtb L4 or L6 infections. We then compared the predicted number of IFN-γ-inducing and HLA high-binding-affinity peptides in Mtb ancestral, L4, or L6 proteins, considering HLA alleles of high or low frequency in West Africa. Our immunoinformatics approach predicts that non-synonymous substitutions of high variance in Mtb L6 strains diminish binding affinities to HLA alleles prevalent in West African populations, suggesting specific adaptations of these strains to their preferred hosts. Future functional studies will advance our knowledge on lineage-specific evolution and inform strategies to enhance TB control in endemic regions. Full article

Before the Anthropocene, Bedouin communities in Southern Israel were based on a clan structure—a kin-based social network; clans were culturally and socially homogenous communities with a strong authority structure. Work consisted of subsistence activities necessary for physical survival. Group-based authority and cooperative problem solving were adaptive in this ecology. Throughout the Anthropocene, the Bedouin of Southern Israel have had to adapt to diverse urban environments, expanded educational opportunity, and exposure to media emanating from different cultures. Our study explored the implications of these ecological shifts for epistemic thinking by comparing three generations of 60 Bedouin families: adolescent girls, their mothers, and their grandmothers (N = 180). Families were evenly divided among three residence types differing in degree of urbanization and degree of population homogeneity: unrecognized Bedouin villages consisting of single clans; recognized Bedouin villages, towns, or cities, consisting of multiple clans; and ethnically diverse cities. Results: Across the generations, media exposure and formally educated parents have weakened the epistemic authority of family elders, in turn weakening clan identity. Ethnically diverse cities have weakened extended family identity. At the same time, personal knowledge and professional expertise have gained new cultural importance. These changes in epistemology and identity are adaptive in the ecological environments that have multiplied in the Anthropocene era. Local identity was strongest both in diverse cities, with their many attractions, and in unrecognized villages, where the population continues to occupy ancestral lands. Full article

The Kelch-like ECH-associated protein 1 (KEAP1)/Nuclear factor E2-related factor 2 (NRF2) pathway is a key mechanism that responds to oxidative stress and xenobiotic stimuli in vertebrates. However, knowledge of its evolutionary origins remains limited. In this study, we identify the ancestral homologues of KEAP1 and NRF (BjKEAP1 and BjNRF) in cephalochordate amphioxus (Branchiostoma japonicum). BjNRF uniquely combines the feature domains of vertebrates NRF1 and NRF2, marking it as an evolutionary intermediate. High expression levels of Bjkeap1 and Bjnrf in the gill, hepatic cecum, and intestine highlight their roles in environmental defense at key interface tissues. Functional studies reveal that BjKEAP1 regulates the cytoplasmic localization of BjNRF. Typical NRF2 activator sulforaphane (SFN) induces its nuclear translocation and significantly elevates the transcriptional expression of BjNRF and phase II detoxification enzymes. Moreover, exposure to the environmental toxin Benzo[a]pyrene (BaP) activates this stress response system. These findings bridge critical gaps in our understanding of this pathway in basal chordates and offer new insights into the evolutionary trajectory of the KEAP1-NRF system. Furthermore, this study highlights crucial implications for the conservation of amphioxus in deteriorating marine environments. Full article

This article ‘Ngā Kare-ā-roto: The Ripples Within’ provides an overview of findings related to Māori views, understanding and expressions of emotions through a Māori cultural lens. One of the key findings from the research project ‘Ngā Kare-ā-roto’ highlighted that emotions are expressed through a range of cultural practices that have served to bring collective understandings to support emotional wellbeing for Māori. At the centre of this research is the revitalisation of ancestral knowledge and practices to support emotional expression. The social and cultural significance of this project is highlighted by Indigenous researchers who have noted the direct relationship of emotions such as grief and trauma to wellbeing. This article provides an outline of kaikōrero (speakers/participants) views on the place of whakataukī (proverbial sayings) in the expression of emotions and emotional states of being. We begin with an overview of the significance of this work to supporting wellbeing and healing. We then move to providing a range of examples that were shared throughout the project that give insights into the ways in which emotions for Māori are framed within our ancestral and cultural understandings, in particular in regards to collective expressions of tikanga (cultural practices) and whakataukī that have been handed down through te reo Māori (Māori language) as sources of knowledge, guidance and learning. Full article

This article offers insights into conceptualizing a different angle of cultural heritage in its intangible form and generational inheritance, in relation to migrant community bonds and their impacts on embodied stories of trauma and healing. This article aims to contribute to understanding how cultural and historical knowledge of heritage is passed from one generation to the next, with deep emotional impacts, whether trauma or self-development. While engaging in an interdisciplinary dialogue with Bion’s work, we explore nodes of divergence and convergence in how gendered and embodied migrant sexuality/identity stories of trauma and healing exemplify the call for research to engage with perspectives of social and cultural differences. This understanding of contested cultural heritage and how belonging can be achieved links to ethnic–ancestral/national consciousness, as well as the struggle to belong among first- and second-generation migrants. The empirical data draws from extensive ethnographic, multi-method, multi-sited, comparative, and narrative research conducted with first- and second-generation migrants. The analysis is situated within Bion’s theory and articulated through an interpretative interdisciplinary framework aiming to unravel the complexity of the phenomena of mobility and identity construction. This analysis exemplifies the power dynamics inherent in migrant inter/intragenerational relations shaped by cultural heritage. Full article

The Bazhou yak, a major native meat yak breed in Xinjiang, China, is renowned for its fast growth rate, strong adaptability, and particularly high intramuscular fat (IMF) content. However, limited knowledge regarding its phylogenetic history and genomic composition has hindered its long-term conservation and utilization. This study evaluated the genetic diversity, population phylogenetics, and genome-wide selective sweep analysis (GWSA) of 100 newly obtained Bazhou yaks through genome resequencing, as well as 340 public yak genomes from nine other populations on the Qinghai–Tibet Plateau. The results revealed moderate diversity, lower genomic inbreeding levels, and rapid linkage disequilibrium (LD) decay in Bazhou yaks. Principal component analysis (PCA) and phylogenetic analysis showed a clear separation of Bazhou yaks from other yak populations, indicating the Bazhou yak as an independent genetic population. Furthermore, less genetic differentiation was found between the Bazhou yak and the Huanhu yak, while ADMIXTURE analysis revealed a common ancestral lineage between Bazhou yaks and Huanhu yaks, indicating an important genetic contribution of the Qinghai yak population to Bazhou yaks. The GWSA identified a total of 833 selected genes in Bazhou yaks using the top 5% interaction windows of both parameters (F_ST, Pi ratio, and XP-EHH). A significant number of these genes are related to fat synthesis and deposition, such as MTOR, APOA1, and GPAT4. In summary, this study sheds light on the phylogenetic status and distinctive genomic features of Bazhou yaks, which facilitates our understanding of the genetic basis of the IMF phenotype in Bazhou yaks. Full article