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Keywords = amylose/amylopectin

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20 pages, 6727 KB  
Article
Optimized Sowing Date and Seeding Rate for Simultaneous Improvement of Yield and Quality of Late-Sown Winter Wheat in the Southern North China Plain
by Shuxian Li, Juan Han and Shiju Liu
Agronomy 2026, 16(14), 1378; https://doi.org/10.3390/agronomy16141378 - 20 Jul 2026
Viewed by 245
Abstract
Sowing date and seeding rate are key agronomic measures for regulating yield formation and quality accumulation in winter wheat, especially in the context of global warming, which has led to delayed sowing becoming the norm. Clearly identifying optimal combinations of sowing date and [...] Read more.
Sowing date and seeding rate are key agronomic measures for regulating yield formation and quality accumulation in winter wheat, especially in the context of global warming, which has led to delayed sowing becoming the norm. Clearly identifying optimal combinations of sowing date and seeding rate under late-sowing conditions is crucial for achieving coordinated improvement in both high yield and quality. Therefore, this study used the strong-gluten wheat cultivar ‘Shaannong 33’ and conducted a two-factor split-plot field experiment in the southern North China Plain (Nanyang, Henan) during 2019–2021. Three sowing dates (early sowing: 27 October; intermediate sowing: 31 October–2 November; late sowing: 6–8 November) and four seeding rates (180 × 104, 240 × 104, 300 × 104, and 360 × 104 plants ha−1) were tested to systematically analyze the effects of sowing date and seeding rate on wheat yield and its components, grain processing quality, and starch physicochemical properties. The results showed that sowing from late October to early November (approximately 25 October to 2 November in 2019–2020 and 25 October to 31 October in 2020–2021) combined with a seeding rate of 240–300 × 104 plants ha−1 maintained yield at a comparably high level across both growing seasons while improving processing quality indicators such as protein content, wet gluten content, and dough stability time for the strong-gluten cultivar ‘Shaannong 33’. However, the optimal sowing window varied between years: in 2019–2020, both D1 (27 October) and D2 (2 November) produced comparable yields, whereas in 2020–2021, D2 (31 October) was superior for both yield and quality. This inter-annual variability highlights the influence of climatic conditions on the optimal sowing window. Further delaying the sowing date to after 6 November led to decreases in yield and quality parameters; however, increasing the seeding rate to 360 × 104 plants ha−1 partially compensated for yield loss. In addition, late sowing significantly increased amylopectin content, swelling power, and pasting indicators such as peak viscosity and final viscosity while decreasing amylose content and pasting temperature, which is beneficial for improving cooking and eating quality. Correlation analysis indicated that the amylose/amylopectin ratio was the core factor determining starch pasting properties, while protein content showed a significant negative correlation with starch pasting indicators, reflecting the resource competition effect between grain protein and starch. Yield differences between the two years were mainly driven by changes in spike number per unit area, while inter-annual climate fluctuations (especially precipitation and temperature) significantly affected spike formation capacity. Overall, sowing date and seeding rate have a synergistic regulatory effect on yield and quality of late-sown wheat for the strong-gluten cultivar ‘Shaannong 33’. It is recommended to adopt a sowing date of 25–31 October with the seeding rate controlled at 240–300 × 104 plants ha−1 to achieve synergistic improvement of yield and quality under late-sowing conditions in the southern North China Plain for this cultivar. Full article
(This article belongs to the Section Soil and Plant Nutrition)
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19 pages, 3193 KB  
Article
Short-Range Starch Order, Pasting Behavior, and Gelatinization of Native and Sprouted Wheat and Rice Flour Systems
by Paloma Lopez-Sarmiento and Julián de la Rosa-Millán
Polysaccharides 2026, 7(3), 85; https://doi.org/10.3390/polysaccharides7030085 - 14 Jul 2026
Viewed by 221
Abstract
This study evaluates the effects of germination on the composition, starch architecture, thermal profiles, short-range molecular order, and pasting behavior of wheat and rice flour systems and their corresponding isolated starch fractions. Germination significantly reduced total starch content in wheat flour (74.81–56.74%) and [...] Read more.
This study evaluates the effects of germination on the composition, starch architecture, thermal profiles, short-range molecular order, and pasting behavior of wheat and rice flour systems and their corresponding isolated starch fractions. Germination significantly reduced total starch content in wheat flour (74.81–56.74%) and rice flour (85.55–64.00%). Additionally, amylose content decreased from 24.70 to 18.77% in wheat flour and from 20.83 to 19.69% in rice flour. Amylopectin A-chains increased in germinated wheat flour (33.98%) and germinated rice flour (31.49%), suggesting starch depolymerization and molecular restructuring. These structural changes are associated with lower gelatinization enthalpy values, which decreased from 8.82 to 4.78 J/g in wheat flour and from 9.99 to 6.90 J/g in rice flour, reflecting reduced thermal stability after sprouting. ATR-FTIR analysis showed that germinated flours had the highest short-range molecular order (1047/1023 ratios of 0.75 and 0.71 for wheat and rice, respectively), despite their lower starch content. Germination also affected the development of viscosity; peak viscosity decreased from 2482.5 to 217.0 cP in wheat flour and from 4830.0 to 1039.5 cP in rice flour. Germination influenced protein secondary structure: it increased relative α-helix content in flour systems (from 35.23% to 36.16% in wheat, and from 36.26% to 37.58% in rice) but decreased it in isolated starches (from 42.05% to 33.17% in wheat, and from 39.60% to 36.29% in rice), suggesting a matrix-specific response. Germination-induced depolymerization and reorganization altered starch structure, thermal properties, and viscosity. These findings provide an integrated structural and functional framework that links starch architecture, molecular order, gelatinization, and the development of viscosity in germinated cereal matrices for food applications. Full article
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15 pages, 1007 KB  
Article
Effects of Dietary Standardized Ileal Digestible Lysine and Amylose/Amylopectin Ratio on Intestinal Morphology, Barrier-Related Gene Expression, and Cecal Microbiota in Broilers Fed Low-Protein Diets
by Minhao Zhang and Jianmin Yuan
Animals 2026, 16(12), 1914; https://doi.org/10.3390/ani16121914 - 20 Jun 2026
Viewed by 370
Abstract
This study investigated the effects of dietary standardized ileal digestible (SID) lysine levels and amylose to amylopectin ratios on the intestinal health of broilers fed an 18.5% crude protein diet from 22 to 42 days of age. A total of 540 healthy male [...] Read more.
This study investigated the effects of dietary standardized ileal digestible (SID) lysine levels and amylose to amylopectin ratios on the intestinal health of broilers fed an 18.5% crude protein diet from 22 to 42 days of age. A total of 540 healthy male Ross 308 broilers were randomly assigned to nine treatments in a 3 × 3 factorial design consisting of three SID lysine levels (1.00%, 1.20%, and 1.40%) and three AM/AP ratios (0.19, 0.29, and 0.41), with six replicates of 10 birds each. Ileal morphology, intestinal barrier function and inflammation-related gene expression, and the composition of cecal microbiota were evaluated. Significant interactions between lysine level and AM/AP ratio were observed for Occludin, ZO-1, Claudin-1, and TNF-α expression, with the highest expression in the 1.40% lysine + 0.41 AM/AP group and the lowest in the 1.00% lysine + 0.19 AM/AP group. The VH/CD ratio showed a significant interaction, with the highest value in the 1.20% lysine + 0.19 AM/AP group and the lowest in the 1.40% lysine + 0.41 AM/AP group. IL-18 and IL-10 were primarily affected by the main effects of lysine and AM/AP ratio. The expression levels of both IL-10 and IL-18 increased with increasing lysine level and increasing starch AM/AP ratio. Dietary SID lysine level and AM/AP ratio interactively regulate the expression of barrier-related genes, inflammatory status, intestinal morphology, and cecal microbiota, potentially contributing to enhanced intestinal health in broilers. However, because microbial metabolites were not measured, the functional significance of the observed microbiota alterations remains speculative. In broilers fed an 18.5% CP diet, a combination of 1.20% SID lysine with an AM/AP ratio of 0.19 was identified as the optimal strategy for maintaining intestinal morphology from 22 to 42 days of age. Full article
(This article belongs to the Section Poultry)
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29 pages, 8738 KB  
Review
Protein–Carbohydrate Interactions in Food Matrices and Their Effects on Food Quality
by Muhammad Arif Ramzan, Anna Wang, Ligen Wu and Muhammad Abdul Haseeb
Foods 2026, 15(12), 2213; https://doi.org/10.3390/foods15122213 - 19 Jun 2026
Viewed by 713
Abstract
The structure, functionality, nutritional value, and sensory properties of food are significantly influenced by interactions between proteins and carbohydrates. These interactions occur through hydrogen bonding, electrostatic forces, hydrophobic interactions, and, in many cases, the covalent attachment of sugars to proteins via the Maillard [...] Read more.
The structure, functionality, nutritional value, and sensory properties of food are significantly influenced by interactions between proteins and carbohydrates. These interactions occur through hydrogen bonding, electrostatic forces, hydrophobic interactions, and, in many cases, the covalent attachment of sugars to proteins via the Maillard reaction. High starch content in food matrices promotes interactions between proteins and starch components such as amylose and amylopectin, affecting gelation, retrogradation, and thickening. These interactions improve shelf stability and product quality. Additionally, protein–carbohydrate interactions regulate nutrient digestibility and glycemic response, playing a crucial role in the development of functional foods for diabetes and weight management. In silico studies have demonstrated that dietary fibers like pectin and cellulose can improve water retention and textural properties in processed meat products. Furthermore, processing techniques such as enzymatic hydrolysis, fermentation, pulsed electric fields (PEF), and low-temperature drying have been found to improve the functional properties and shelf life of food products. This review synthesizes recent findings on protein–carbohydrate interactions and highlights their potential in creating healthier, more appealing, and sustainable foods that align with modern consumer preferences. Full article
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24 pages, 15145 KB  
Article
Effect of Resistant Dextrin on the Functional, Thermal and Structural Properties of Cooked Chinese Rice
by Ruijun Chen, Qiuling Tang, Shiyu Chang, Barbara Conti and Xingjun Li
Gels 2026, 12(6), 516; https://doi.org/10.3390/gels12060516 - 10 Jun 2026
Viewed by 229
Abstract
This study added two types of resistant dextrin (RD), i.e., Bailong (BL) and Luo Gaite (LGT)) to a Japonica (cv. RXY) and an early indica (cv. IP44) rice during cooking and analysed the functional and structural properties of the cooked rice. Compared with [...] Read more.
This study added two types of resistant dextrin (RD), i.e., Bailong (BL) and Luo Gaite (LGT)) to a Japonica (cv. RXY) and an early indica (cv. IP44) rice during cooking and analysed the functional and structural properties of the cooked rice. Compared with no RD addition, 3–10% RD addition induced a declinein cooking time and an incrementin gruel solid loss. Further, 3–10% RD addition increased the hardness, chewiness, and springiness of cooked rice but decreased the cohesiveness. With increases in the added RD amount, the smell, structural appearance, palatability, taste, cool rice texture, and total score of the cooked rice all increased; the peak time and pasting temperature increased, but the peak, final, breakdown, and setback viscosities all significantly decreased. The enthalpy, conclusion temperature of gelatinisation, and gelatinisation peak width and height all decreased with increasing RD amount, but the peak temperature of gelatinisation increased. The addition of 3–7% RD did not change amylopectin ageing, but 10% RD significantly increased amylopectin ageing. RD addition reduced the protein weakness degree and starch breakdown torque of rice doughbut appeared to increase the amorphous and crystalline regions of cooked rice. The addition of 10% BL or LGT induced the formation of α-helix and random coil secondary protein structures in cooked rice, with optimal cooking properties and total sensory score. Microstructure analysis further showed that low-viscous RD induced the formation of new gel-like structures. In conclusion, 3–10% RD addition in cooking rice decreases amylose recrystallisation, weakens the protein structure, and induces new gel-like structures, enhancing the hardness, chewiness, adhesiveness, springiness, and sensory score of cooked rice. This study is useful for developing functionalcooked rice. Full article
(This article belongs to the Special Issue Advanced Gels in the Food System (2nd Edition))
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23 pages, 3872 KB  
Article
Comparison of the Structure and Properties of Hydroxypropyl Starch/Carrageenan Blends with Different Amylose/Amylopectin Contents
by Xingxing Zhu, Di Wu, Juanjuan Wu, Jinglong Zhao, Yunhe Lian and Yunkai Lv
Gels 2026, 12(5), 423; https://doi.org/10.3390/gels12050423 - 12 May 2026
Viewed by 592
Abstract
To compare the structure and properties of hydroxypropyl starch/carrageenan blends with different amylose/amylopectin contents, two types of hydroxypropyl starch—a high-amylose type (amylose content > 70%) and a high-amylopectin type (amylopectin content > 95%)—were used. These starches had similar molecular weights, degrees of hydroxypropyl [...] Read more.
To compare the structure and properties of hydroxypropyl starch/carrageenan blends with different amylose/amylopectin contents, two types of hydroxypropyl starch—a high-amylose type (amylose content > 70%) and a high-amylopectin type (amylopectin content > 95%)—were used. These starches had similar molecular weights, degrees of hydroxypropyl substitution, and other properties, differing only in their amylose and amylopectin contents. Each starch was blended with carrageenan via a solution blending method, and the resulting blends were systematically characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric analysis, rheological tests, texture analysis, mechanical property tests, contact angle analysis, and UV-Vis spectrophotometry. The results showed that, upon blending with carrageenan, the hydroxypropyl starch transformed from a weak viscoelastic solution into an elastic, strong gel. FTIR and XRD analyses confirmed that the hydroxypropyl starch and carrageenan formed a homogeneous, compact, three-dimensional network via hydrogen bonding. This significantly enhanced the mechanical strength and stability of the blends. The influence of starch molecular structure on the blend system’s properties exhibited a pronounced state dependence. In the gel state, hydroxypropyl amylopectin effectively filled the carrageenan network due to its high swelling capacity, thereby improving the thermal stability and textural properties of the blends. However, in the film state, hydroxypropyl amylose with higher crystallinity and denser molecular packing contributed to superior tensile strength, hydrophobicity and light transmittance. Furthermore, the optimal mass ratio of hydroxypropyl starch to carrageenan was found to be in the range of 2:1 to 4:1. With this ratio, excessive cross-linking and poor compatibility could be avoided, resulting in improved mechanical performance, hydrophobicity, and light transmittance. This study reveals the relationship between starch molecular structure, system state and macroscopic properties, providing a theoretical basis for the rational design and regulation of the properties of hydroxypropyl starch/carrageenan blends. Full article
(This article belongs to the Section Gel Analysis and Characterization)
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19 pages, 4749 KB  
Article
Responses of Japonica Rice Quality Indicators and Starch Properties to Low Temperature at Different Periods of the Grain-Filling Stage in Cold Regions
by Mingyu Fan, Miao Hou, Fanxu Meng, Wenxuan Dai, Chuanming Yang and Hongyu Li
Foods 2026, 15(8), 1355; https://doi.org/10.3390/foods15081355 - 13 Apr 2026
Viewed by 618
Abstract
Low temperature during grain filling is a major constraint affecting rice quality in cold regions. This study investigated how low temperature influences rice quality and starch characteristics at different periods of the grain-filling stage using two Japonica rice cultivars, Kenjing 7 (KJ7, moderate [...] Read more.
Low temperature during grain filling is a major constraint affecting rice quality in cold regions. This study investigated how low temperature influences rice quality and starch characteristics at different periods of the grain-filling stage using two Japonica rice cultivars, Kenjing 7 (KJ7, moderate stress tolerance) and Kenjing 8 (KJ8, strong stress tolerance). Low-temperature treatments (17/13 °C, day/night) were applied during the early (5–11 days after anthesis), middle (12–18 days), and late (19–25 days) grain-filling stages and milling, appearance, nutritional, eating and cooking qualities as well as starch physicochemical properties were evaluated. Responses differed markedly between cultivars and treatment periods. Under low-temperature conditions, brown rice and milled rice rates of KJ8 increased during the early and middle grain-filling stages, whereas those of KJ7 declined during the late stage. Low-temperature stress increased protein, total starch, and amylose contents, while reducing gel consistency and the taste value of KJ7. Grain chalkiness increased significantly during the late stage, whereas during the early and middle stages, grain chalkiness, peak viscosity, and breakdown decreased and setback increased. Low temperature increased starch granule size and the proportions of short and intermediate chains of amylopectin, reduced medium-long and long chain and relative crystallinity, without altering starch crystalline type, and produced uneven starch particle surfaces with small pores. These effects were most pronounced during the late grain-filling stage. Overall, low temperature altered starch content and structure, thereby modifying pasting properties and ultimately leading to differences in rice quality. Full article
(This article belongs to the Section Food Quality and Safety)
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22 pages, 6313 KB  
Article
Effects of Nitrogen Fertilizer Levels on Rice Quality and Starch Properties of Common and Glutinous Japonica Rice: Implications for Sustainable Nitrogen Management
by Dongxu Han, Baiwen Jiang and Xingyu You
Sustainability 2026, 18(8), 3828; https://doi.org/10.3390/su18083828 - 13 Apr 2026
Viewed by 556
Abstract
Optimizing nitrogen (N) fertilizer application within conventional rice production systems remains essential for improving grain quality while avoiding inefficient resource use. This study examined how different N application levels influence rice quality, starch structure, and physicochemical properties in two japonica rice types cultivated [...] Read more.
Optimizing nitrogen (N) fertilizer application within conventional rice production systems remains essential for improving grain quality while avoiding inefficient resource use. This study examined how different N application levels influence rice quality, starch structure, and physicochemical properties in two japonica rice types cultivated under cold-region conditions in Northeast China. Using two cultivars, common japonica rice ‘Putian 1498’ and glutinous japonica rice ‘Longjing 57’, four nitrogen levels were established under machine-transplanting conditions: N0 (0 kg/hm2), N1 (80 kg/hm2), N2 (135 kg/hm2), and N3 (190 kg/hm2). The results indicate that increasing nitrogen application differentially affected the milling quality of the two rice types: it reached its maximum at the N1 level for common japonica rice and at the N3 level for glutinous japonica rice. However, the taste value decreased and chalkiness increased in both types. Regarding starch properties, increased nitrogen application led to rougher starch granule surfaces, a decrease in large granules, and an increase in medium and small granules. Starch content decreased, and the amylose-to-amylopectin ratio declined. Relative crystallinity increased, while the FTIR ratio of 1045/1022 cm−1 decreased. Solubility showed an increasing trend, whereas swelling power exhibited the opposite trend. The gelatinization enthalpy and pasting temperatures were positively correlated with nitrogen rate, whereas retrogradation degree showed a negative correlation. These results demonstrate that nitrogen application regulates rice quality through changes in starch structure and physicochemical properties, with distinct responses between common and glutinous japonica rice. Moderate nitrogen input improves milling quality, but excessive application reduces eating quality, indicating a trade-off between processing performance and consumer-oriented quality. This study provides mechanistic evidence to support more precise nitrogen management in conventional rice systems, contributing to improved resource-use efficiency without overstating broader sustainability claims. In conclusion, moderate nitrogen application optimizes rice quality by balancing milling performance and eating quality through its effects on starch structure, whereas excessive nitrogen input leads to quality deterioration and inefficient resource use. Full article
(This article belongs to the Section Sustainable Agriculture)
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18 pages, 2467 KB  
Article
Physicochemical, Pasting and Thermal Properties of the Starch of Three Varieties of Yam (Dioscorea spp.) as Potential Food Ingredients
by Mildreth Cordero-Herrera, José Benítez-Lobo, Claudia De Paula, Ricardo Andrade-Pizarro, Piedad Montero-Castillo, Diofanor Acevedo-Correa, Jhon Rodríguez-Meza and Alba Durango-Villadiego
Polymers 2026, 18(8), 943; https://doi.org/10.3390/polym18080943 - 12 Apr 2026
Viewed by 1138
Abstract
Yam starch accounts for 70–80% of its dry matter, and its physicochemical and technofunctional properties are crucial for its use in the food industry (gelling agent, thickener, and stabilizer). The objective of this study focuses on the physicochemical, pasting and thermal properties of [...] Read more.
Yam starch accounts for 70–80% of its dry matter, and its physicochemical and technofunctional properties are crucial for its use in the food industry (gelling agent, thickener, and stabilizer). The objective of this study focuses on the physicochemical, pasting and thermal properties of starch extracted from the yam varieties Dioscorea cayenensis, Dioscorea alata, and Dioscorea rotundata. The proximal composition, amylose and amylopectin content, as well as their functional properties (absorption index, solubility, swelling, thermal and pasting behavior, morphology, and color) were analyzed. The results showed that the starch extraction yield varied between varieties, being highest in D. cayenensis with 14.14%. D. alata had the highest starch (82.24%) and amylose (34.69%) content, which gives it greater gel firmness and retrogradation potential, as well as the best techno-functional properties water absorption index (2.46 g/g), water solubility index (1.1%), and swelling power (2.54 g/g). D. cayenensis stands out for its high amylopectin content (69.62%) and brightness (96.89), reflecting greater starch whiteness. D. rotundata has an intermediate balance between amylose and amylopectin, which makes it versatile. The proximal composition and techno-functional properties of yam starch position it as a promising raw material for the food industry, especially in the manufacture of thickeners, gelling agents, and in bakery products, pasta and noodles. Full article
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17 pages, 10135 KB  
Article
Physicochemical Properties of Starch from High-Quality Hybrid Indica Rice: Insights from National High-Quality Rice Gold Award Chinese Varieties
by Yumei Wang, Jiale Wu, Xingeng Wu, Yanhua Zeng, Yongjun Zeng, Feiyu Tang and Xiaobing Xie
Foods 2026, 15(8), 1335; https://doi.org/10.3390/foods15081335 - 11 Apr 2026
Viewed by 547
Abstract
The physicochemical properties of starch in high-quality hybrid indica rice (HQR) varieties that have received the National High-Quality Rice Gold Award are not well characterized. Ten HQR and two ordinary-quality indica rice (OQR) varieties were selected for this study. All varieties were identically [...] Read more.
The physicochemical properties of starch in high-quality hybrid indica rice (HQR) varieties that have received the National High-Quality Rice Gold Award are not well characterized. Ten HQR and two ordinary-quality indica rice (OQR) varieties were selected for this study. All varieties were identically cultivated under late-season conditions in southern China and were subsequently analyzed for differences in taste-related attributes, amylopectin fine structure, and functional properties. Compared with OQR varieties, HQR varieties exhibited a distinct starch profile: lower amylose (16.6–20.2%) but higher amylopectin content (62.6–65.0%), a greater proportion of small and medium starch granules, and a higher ratio of A and B1 chains in amylopectin (with few exceptions). Functionally, HQR varieties showed significantly (p < 0.05) higher gel consistency, solubility, and swelling power, along with higher breakdown but lower setback. They also generally exhibited higher crystallinity and gelatinization enthalpy, alongside a softer texture. Notably, the functional properties showed strong correlations (p < 0.05) with most taste-related attributes and amylopectin fine structures across all varieties. These findings provide critical guidance for future breeding programs aimed at improving the quality of indica rice and developing new elite HQR varieties. Full article
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23 pages, 2315 KB  
Article
Carbon–Nitrogen Metabolism Associated with Appearance Quality in Superior and Inferior Grains of Soft and Non-Soft Japonica Rice in Southern China
by Xi Chen, Jianghui Yu, Ying Zhu, Guodong Liu, Guangyan Li, Fangfu Xu, Qun Hu, Jiale Cao, Hongcheng Zhang and Haiyan Wei
Plants 2026, 15(8), 1155; https://doi.org/10.3390/plants15081155 - 9 Apr 2026
Viewed by 527
Abstract
To investigate the differences in carbon and nitrogen metabolism between superior and inferior grains of southern soft and non-soft japonica rice and their relationships with appearance quality, the metabolic characteristics and appearance quality of superior and inferior grains during the grain-filling stage were [...] Read more.
To investigate the differences in carbon and nitrogen metabolism between superior and inferior grains of southern soft and non-soft japonica rice and their relationships with appearance quality, the metabolic characteristics and appearance quality of superior and inferior grains during the grain-filling stage were compared between the two rice types. The results showed that, compared with non-soft japonica rice, the activities of AGPase and GBSS in superior grains of soft rice were significantly lower, whereas the activities of SSS, SBE, and DBE were significantly higher. The amylose content decreased by 32.68–44.72%, while amylopectin increased by 7.27–10.73%. The limitation in carbon metabolism was more pronounced in inferior grains, and the non-structural carbohydrate content was 9.33–17.33% lower than that in superior grains. In terms of nitrogen metabolism, GS activity decreased whereas GOGAT activity increased in superior grains, resulting in a 6.28–8.38% increase in protein content. The protein content of inferior grains was 1.75–6.44% higher than that of superior grains. In addition, the chalky grain rate and chalkiness degree of superior grains in soft rice were 79.00–481.03% higher than those in non-soft japonica rice, while the increases in inferior grains ranged from 67.51% to 136.31%. Correlation analysis indicated that the chalky grain rate of superior grains was positively correlated with starch content during the early grain-filling stage, whereas the chalkiness degree of inferior grains was positively correlated with protein content. These results suggest that differences in carbon and nitrogen metabolism between grain positions are closely associated with the formation of appearance quality. Full article
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22 pages, 13137 KB  
Article
Effects of High Temperature and Nitrogen Fertilizer on the Carbon and Nitrogen Metabolism Characteristics of Rice Varieties with Differing Taste Stability
by Ke Ma, Yuanyuan Zhou, Yao Ma, Zexin Qi and Heping Xu
Plants 2026, 15(7), 1006; https://doi.org/10.3390/plants15071006 - 25 Mar 2026
Viewed by 621
Abstract
Temperature and nitrogen fertilizer are key environmental factors that significantly affect rice growth and grain quality. There remains a lack of systematic research on the effects of temperature and nitrogen fertilizer on carbon–nitrogen metabolism during grain-filling, and consequently on the taste quality of [...] Read more.
Temperature and nitrogen fertilizer are key environmental factors that significantly affect rice growth and grain quality. There remains a lack of systematic research on the effects of temperature and nitrogen fertilizer on carbon–nitrogen metabolism during grain-filling, and consequently on the taste quality of rice varieties with different taste characteristics. To bridge this gap, pot experiments were conducted under different temperature and nitrogen fertilizer conditions to investigate the changes in carbon and nitrogen metabolism and the quality of different high-quality and stable-taste rice varieties during the grain filling stage. Our research results indicate that high-temperature conditions inhibit both carbon and nitrogen metabolism; however, the variations differ among rice varieties with differing taste stability. Under both normal and high nitrogen levels, compared to Akita Komachi (AK), a variety with poor taste stability, Jikedao 606 (J 606), a variety with strong taste stability, maintained a certain photosynthetic capacity under high-temperature conditions, with smaller decreases in net photosynthetic rate and soil–plant analysis development values, declining by 4.30–5.59% and 4.30–5.59% respectively. The decline in the activities of nitrate reductase, glutamine synthetase, and glutamate synthase in nitrogen metabolism was relatively small; in comparison, the decrease in the activities of ADP-glucose pyrophosphorylase, granule-bound starch synthase, starch branching enzyme, and starch debranching enzyme in carbon metabolism was comparatively minor. The content of amylose and amylopectin in the grains was maintained, improving the milled rice rate and head rice rate, thereby ensuring strong stability of excellent sensory quality. Under both high-temperature and high-nitrogen conditions, the yields of the two rice varieties were maintained. In summary, variations exist in carbon and nitrogen metabolism among different rice varieties with stable excellent taste under varying temperature and nitrogen fertilizer conditions. These metabolic differences affect starch synthesis in the endosperm, ultimately influencing the stability of rice sensory quality. This study provides a theoretical basis for nitrogen fertilizer application under high-temperature conditions and the cultivation of rice varieties with excellent taste stability. Full article
(This article belongs to the Special Issue Genetic and Metabolic Insights into Crop Improvement)
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13 pages, 1132 KB  
Article
Impact of Storage Duration on the Structural and Functional Properties of Starch in Spicy Strips
by Yujing Ding, Hongling Chao, Xiutian Li, Yang Li, Mingfei Li, Xiaowei Zhang, Shiyuan Miao, Yujie Lu and Dube Nhlanhla Mtelisi
Foods 2026, 15(5), 826; https://doi.org/10.3390/foods15050826 - 2 Mar 2026
Cited by 1 | Viewed by 523
Abstract
The effects of storage time on the characteristics of starch in spicy strips were investigated. Techniques including differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and X-ray diffraction (XRD) were employed to analyze the gelatinization properties, thermal characteristics, crystal structure, moisture distribution, and quality [...] Read more.
The effects of storage time on the characteristics of starch in spicy strips were investigated. Techniques including differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and X-ray diffraction (XRD) were employed to analyze the gelatinization properties, thermal characteristics, crystal structure, moisture distribution, and quality changes of spicy strips under different storage periods (0, 60, 120, and 180 days). The results demonstrated that prolonged storage led to a significant decrease in peak viscosity and an increase in setback value, indicating enhanced starch retrogradation. DSC analysis revealed a continuous increase in enthalpy change (ΔH), confirming the formation of more ordered double-helix structures over time. TGA revealed a shift in thermal degradation profiles, indicating changes in component interactions and moisture-binding capacity over storage. XRD patterns showed a clear transition from A-type to V-type crystals and finally to an amorphous state after 180 days. Consequently, solubility, swelling power, and amylose leaching were markedly inhibited, while the retrogradation rate of amylopectin became dominant during long-term storage. These findings provide insights into starch retrogradation mechanisms in complex snack matrices and offer guidance on mitigating quality deterioration during the shelf life of spicy strips. Full article
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27 pages, 1329 KB  
Review
Hydrophobicity Strategies of Starch-Based Films: Recent Advances and Perspectives
by Elsa F. Vieira, Tomás Amaral, Valentina F. Domingues and Cristina Delerue-Matos
Polymers 2026, 18(4), 490; https://doi.org/10.3390/polym18040490 - 15 Feb 2026
Cited by 5 | Viewed by 2069
Abstract
The rapid accumulation of plastic waste and the depletion of fossil resources have intensified global efforts to develop biodegradable polymeric materials derived from renewable feedstocks. In this context, starch-based films have emerged as one of the most promising alternatives to conventional petroleum-based plastics, [...] Read more.
The rapid accumulation of plastic waste and the depletion of fossil resources have intensified global efforts to develop biodegradable polymeric materials derived from renewable feedstocks. In this context, starch-based films have emerged as one of the most promising alternatives to conventional petroleum-based plastics, owing to their wide availability, low cost, biodegradability, and ability to form continuous films using simple and scalable processing techniques. Starch is a naturally occurring polysaccharide composed primarily of amylose and amylopectin, whose molecular structure is rich in hydroxyl (–OH) groups. These functional groups promote extensive intermolecular hydrogen bonding, enabling starch gelatinization and film formation in aqueous systems. However, the same hydroxyl-rich structure confers a pronounced hydrophilic character, resulting in high moisture sensitivity, poor water vapor barrier properties, and limited dimensional stability under humid. Consequently, improving the hydrophobicity of starch-based films remains one of the most critical challenges for their practical application in food packaging. This review aims to summarize and critically discuss the main strategies reported for improving the hydrophobicity of starch-based films. The review focuses on composition and processing approaches, including (i) chemical modification of starch, (ii) incorporation of hydrophobic additives, (iii) reinforcement with natural fibers and nanocellulosic materials, (iv) polymer blending and multilayer/gradient architectures, and (v) processing strategies, including film homogenization, shear treatment and aging conditions. Emphasis is placed on the mechanisms governing hydrophobicity enhancement, comparative performance indicators, and current limitations. Full article
(This article belongs to the Special Issue Sustainable Polymers in Waste Management and Recycling)
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20 pages, 3995 KB  
Article
Role of Starch Type in Gel-like Network Formation of Extruded Meat Analogs
by Chaeyeon Kang, Ayeon Han and Bon-Jae Gu
Gels 2026, 12(1), 94; https://doi.org/10.3390/gels12010094 - 22 Jan 2026
Cited by 5 | Viewed by 1250
Abstract
Starches play a crucial role in determining the expansion, texture, and structural development of extruded meat analogs through their gelatinization behavior and interactions with proteins. In this study, corn, pea, tapioca, sweet potato, and potato starches were incorporated into soy protein-based formulations and [...] Read more.
Starches play a crucial role in determining the expansion, texture, and structural development of extruded meat analogs through their gelatinization behavior and interactions with proteins. In this study, corn, pea, tapioca, sweet potato, and potato starches were incorporated into soy protein-based formulations and processed under low-moisture and high-moisture extrusion conditions to investigate starch-dependent physicochemical properties. Amylose/amylopectin composition and starch pasting properties were evaluated, and the resulting extrudates were characterized in terms of expansion behavior, water-related properties, textural attributes, and internal structure. Distinct differences in pasting behavior were observed among starches, with potato starch exhibiting high peak viscosity and pea starch showing strong viscosity development during cooling. These differences were closely associated with extrusion outcomes, influencing expansion ratio and texture formation. In low-moisture extrusion, starches susceptible to thermal and shear degradation showed increased solubilization, whereas in high-moisture extrusion, enhanced starch gelatinization promoted starch–protein interactions and contributed to improved textural integrity and structural alignment. Overall, the results demonstrate that starch type is a key determinant of expansion behavior, texture, and structural organization in extruded meat analogs, highlighting the importance of starch selection and processing conditions for tailoring product quality. Full article
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