Search Results (9)

Seed aging has adverse effects on agricultural production, mainly because seed vigor is inhibited. Spermidine can improve seed vitality and germination ability to a certain extent and is essential for plant growth and plant response to stress. This study explored how spermidine counteracted aging effects on sorghum seed germination through antioxidant metabolism regulation. Aged seeds showed decreased vigor due to heightened reactive oxygen species (ROS) and diminished antioxidants. Applying spermidine notably enhanced aged seeds’ germination and vigor by boosting antioxidant enzyme activity and curbing ROS. Integrated transcriptomic, proteomic, and metabolomic analyses demonstrated that the majority of differentially expressed genes following exogenous spermidine treatment in aged sorghum seeds were significantly enriched in pathways related to glutathione metabolism, phenylpropanoid, and flavonoid biosynthesis, resulting in increased expression of genes encoding peroxidase, chalcone synthase, and glutathione s-transferase. Exogenous spermidine facilitated the synthesis of peroxidases and glutathione transferases. Analysis of flavonoid pathway intermediates showed a notable increase in antioxidant metabolites like isoquercitrin, underscoring their role in oxidative stress resistance. This multi-omics strategy underscores Spd’s role in boosting aged seeds’ antioxidants, highlighting the molecular basis of seed aging and Spd’s rejuvenating impact. Full article

Moniliformin (MON) is a highly polar, emerging Fusarium mycotoxin with a low molecular weight. It is known to exhibit potentially harmful effects on public and animal health. This study aimed to comprehensively assess the natural occurrence of MON in various foods marketed in South Korea and to perform a risk assessment. An analytical method for MON quantification using strong anion exchange clean-up combined with ultra-high-performance liquid chromatography–tandem mass spectrometry was validated across four different food matrices (white rice, sorghum, corn oil, and baby food), exhibiting excellent accuracy, precision, and sensitivity. A total of six food categories, 33 food commodities, and 253 food samples were included in this study. Maize, sorghum, Job’s tears, and perilla seeds were identified as the major contributors to MON contamination. Estimated daily intake (EDI) was calculated for both mean and 95th percentile extreme dietary scenarios using upper and lower bound approaches. The highest EDI was observed in the 0–2-year and 3–6-year age groups, primarily for cereal grains. The margin of exposure (MOE) values for maize consumption ranged from 2544 to 7482. These results highlight the potential health concerns associated with MON, necessitating targeted risk management strategies. Full article

This exploratory study expected crop management and climatic factors to significantly influence the nutrient composition and amino acid digestibility of tannin-free sorghum grain determined in broilers of 3 wks of age. Using data from six tannin-free sorghum samples harvested across the southeast USA, Pearson correlations were analyzed (r ≥ |0.8|; p < 0.05). Standardized ileal amino acid digestibility (SIAD) was determined in a previous study using eight replicate cages with 13 birds per sorghum sample. SIAD values were correlated with nitrogen fertilization, yield, seeding rate, and climatic data obtained by surveying the crop growers and from weather stations. Nitrogen fertilization positively correlated with dry matter and starch. Yield was positively associated with SIAD, while seeding rate was negatively correlated with dry matter and Lys. Fiber, particular neutral detergent fiber, showed an inverse relationship with SIAD. No significant correlations with climatic factors were found, which was likely due to the close proximity of growing locations (r ≤ |0.8|; p > 0.05). Despite the limitations of a small sample size (n = 6) and genetic variability within and between each sorghum sample, these findings provide preliminary insights into managing sorghum cultivation to enhance its nutritional value for poultry. Future research should explore larger datasets, from further locations apart, and standardized data collection measurements to be able develop predictive models for grain quality improvement. Full article

Seed germination (GS) and seedling growth are vital plant stages that can be affected by stresses such as drought and aging, which cause deterioration and reduce seed viability. With the aim of homogenizing and improving GS, priming treatments with biostimulants such as the antioxidant melatonin are commonly used in seeds. In this study, the effects of melatonin on germination and seedling growth in two different situations, i.e., aging seeds of rice, barley, and sorghum and under polyethylene glycol (PEG)-induced drought stress in sorghum, were studied. Aged seeds were primed for 7 days in different concentrations of melatonin, and drought stress seeds were primed for 24 h before PEG treatment for 7 days, and germination and initial growth parameters were monitored. Aging-seeds of rice and barley showed the maximum response in terms of germination percentage at 20 µM melatonin and 0.05 µM respectively; while aging-seeds of sorghum showed improvement in germination for practically all concentrations studied, even the highest tested at 50 µM. Regarding the effect of melatonin treatments on drought stress in sorghum seeds, all the studied parameters showed a significant attenuation of the adverse effects of drought stress, alleviating them, for all concentrations tested but especially at 200 µM melatonin. The results obtained confirm that priming seeds with melatonin under low germinability conditions relieves stress and improves both germination and seedling growth. Full article

Starch, a substance stored in seeds, is the main source of energy for germination in sorghum seeds. However, as the seeds age, the catabolism of seed starch is affected, thereby seriously damaging germination ability. In this study, we aimed to understand how exogenous spermidine promoted germination in aged sorghum seed. Our phenotypic analysis indicated that exogenous spermidine not only significantly improved the germination rate, germination potential, germination index, and vigor index of aged seeds, but also increased the root and shoot length after germination. Further, physiological analysis showed that exogenous spermidine increased the content of soluble sugar by upregulating the activity of amylase and sucrose invertase. Exogenous spermidine also improved the activities of key enzymes in glycolysis, the tricarboxylic acid cycle, and the pentose phosphate pathway of aged sorghum seeds. Interestingly, exogenous spermidine protected the mitochondrial structure of aged seeds, which was consistent with the increase in the respiration rate and ATP content during seed germination. Moreover, qRT-PCR analysis revealed that exogenous spermidine induced the expression of key genes related to starch and sugar metabolism in aged sorghum seeds. In conclusion, our study demonstrated that exogenous spermidine promoted aged sorghum seed germination by regulating starch and sugar metabolism. Full article

Grain sorghum (Sorghum bicolor L.) is a major dryland crop in the Texas High Plains. Currently, drought and infestation by the sugarcane aphid (SCA, Melanaphis sacchari) are the two major challenges to grain sorghum production in the area. A 2-year field study was conducted to investigate the effect of planting date (PD) and hybrid selection on yield, evapotranspiration (ET), water use efficiency (WUE), and SCA infestation. Five sorghum hybrids (86P20, SP-31A15, AG1201, AG1203, and DKS37-07) were grown on two planting dates (PD1—early May; PD2—late June) under dryland conditions. Insecticides were not used. There were significant differences in grain yield, WUE, evapotranspiration (ET), and SCA population between two PDs and among hybrids. For PD1, SCA infestation occurred after sorghum reached physiological maturity in 2017. Although SCA infestation was observed during late grain filling in 2018, SCA populations were low and did not affect yield. For PD2, SCA was present before anthesis in both years and significantly affected grain yield. Even with heavy SCA infestation in PD2, the grain yield was higher in PD2 than in PD1 due to timely precipitation. Among hybrids, AG1203, 86P20 and DK37-07 performed better with higher yield and less SCA infestation in PD2. Grain yield was more related to seeds per plant than to kernel weight and harvest index. Full article

Sorghum (Sorghum bicolor [L.] Moench) is a valuable crop in the dry regions of the world, including Namibia. Due to the intensity and recurrence of drought and heat stress in the traditional sorghum growing areas, there is a need to breed and deploy new generation farmer-preferred and climate-smart cultivars to serve the diverse value chains. Therefore, the objectives of this study were to assess the present state of sorghum production in northern Namibia and document farmers’ perceived production constraints and trait preferences in new varieties to guide drought-tolerance breeding. A survey was conducted using a participatory rural appraisal in the following six selected sorghum-growing constituencies in Namibia: Kapako and Mpungu (Kavango West Region), Eenhana and Endola (Ohangwena Region), and Katima Mulilo Rural and Kongola (Zambezi Region). Data were collected using a structured questionnaire involving 198 farmers in 14 sampled villages across the regions. Results revealed variable trends in sorghum production among respondent farmers when disaggregated by gender, age, number of households, education level, cropping systems, types of varieties grown, and perceived production constraints. An equal proportion of male and female respondent farmers cultivate sorghum, suggesting the value of the crop to both genders in Namibia. Most respondent farmers (63.6%) were in productive age groups of <40 years old. In the study areas, low-yielding landrace varieties, namely Ekoko, Okambete, Makonga, Kamburo, Nkutji, Katoma, Fuba, Dommy, Kawumbe, and Okatombo, were widely cultivated, and most of the farmers did not use chemical fertilizers to cultivate sorghum. Farmers’ perceived sorghum production constraints in the study areas included recurrent drought, declining soil fertility, insect pest damage, high cost of production inputs, unavailability of improved seed, lack of alternative improved varieties with farmers’ preferred traits, lack of organic manure, limited access to market and limited extension service. The key farmers’ preferred traits in a new sorghum variety included high grain yield, early maturity, and tolerance to drought, in the field and storage insect pests. The study recommends genetic improvement and new variety deployment of sorghum with the described farmers-preferred traits to increase the sustainable production of the crop in Namibia. Full article

The interactions between ambient temperatures and host plants are central to the population dynamics of invasive animal species. Despite significant research into the effects of temperatures, the performance of invasive species is also influenced by host plants. The effects of different temperatures (20, 25, and 30 °C) and host plants (maize, sorghum, and coix seed) were tested on the mortality, development, reproduction, and population parameters of the fall armyworm (FAW), Spodoptera frugiperda (J E Smith) (Lepidoptera: Noctuidae), using an age-stage, two-sex life table. The results support the hypothesis that temperature and the species of the host plant significantly influences the performance of FAW. Feeding on maize at 30 °C resulted in a lower mortality rate, a shorter developmental time and longevity, a higher fecundity, intrinsic rate of natural increase (r), finite rate of increase (λ), and net reproductive rate (R₀). However, at 20 °C, the host plant could eliminate temperature-mediated synergism in FAW performance, which did not reach statistical significance at 20 °C. Similar results induced by a relatively low temperature (20 °C) on different host plants were also found in the age-stage specific survival curves (s_xj), fecundity (m_x), maternity (l_xm_x), and reproductive value (v_xj) curves of FAW. Consequently, we also need to pay more attention to FAW outbreaks on different host plants mediated by relatively low temperatures. Full article

Ergot is a disease of cereals and grasses caused by fungi in the genus Claviceps. Of particular concern are Claviceps purpurea in temperate regions, C. africana in sorghum (worldwide), and C. fusiformis in pearl millet (Africa, Asia). The fungi infect young, usually unfertilized ovaries, replacing the seeds by dark mycelial masses known as sclerotia. The percentage of sclerotia in marketable grain is strictly regulated in many countries. In winter rye, ergot has been known in Europe since the early Middle Ages. The alkaloids produced by the fungus severely affect the health of humans and warm-blooded animals. In sorghum and pearl millet, ergot became a problem when growers adopted hybrid technology, which increased host susceptibility. Plant traits reducing ergot infection include immediate pollination of receptive stigmas, closed flowering (cleistogamy), and physiological resistance. Genetic, nonpollen-mediated variation in ergot susceptibility could be demonstrated in all three affected cereals. Fungicides have limited efficacy and application is weather dependent. Sorting out the sclerotia from the harvest by photocells is expensive and time consuming. In conclusion, molecular-based hybrid rye breeding could improve pollen fertility by introgressing effective restorer genes thus bringing down the ergot infection level to that of conventional population cultivars. A further reduction might be feasible in the future by selecting more resistant germplasm. Full article