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Search Results (333)

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Keywords = adhesion to dentin

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19 pages, 4325 KiB  
Article
The Impact of Nanoparticle Coatings on the Color of Teeth Restored Using Dental Adhesives Augmented with Magnetic Nanoparticles
by Carina Sonia Neagu, Andreea Codruta Novac, Cristian Zaharia, Meda-Lavinia Negrutiu, Izabell Craciunescu, Vlad Mircea Socoliuc, Catalin Nicolae Marin, Ionela-Amalia Bradu, Luminita Maria Nica, Marius Stef, Virgil-Florin Duma, Mihai Romînu and Cosmin Sinescu
Medicina 2025, 61(7), 1289; https://doi.org/10.3390/medicina61071289 - 17 Jul 2025
Viewed by 326
Abstract
Background and Objectives: Dental adhesives augmented with magnetic nanoparticles (MNPs) have been proposed to prevent microleakages. MNPs dispersed in a dental adhesive reduce the thickness of the adhesive layer applied in a magnetic field and enhance the bond strength by favoring the [...] Read more.
Background and Objectives: Dental adhesives augmented with magnetic nanoparticles (MNPs) have been proposed to prevent microleakages. MNPs dispersed in a dental adhesive reduce the thickness of the adhesive layer applied in a magnetic field and enhance the bond strength by favoring the penetration of the adhesive into dentinal tubules. However, the restoration’s color has been found to be affected by the MNPs. This study tests the hypothesis that MNP coating can alleviate the esthetic impact of magnetic dental adhesives. Materials and Methods: We synthesized Fe3O4 MNPs with silica coating (MNPs-SiO2), calcium-based coating (MNPs-Ca), and no coating. Their morphology was studied using transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Their chemical composition was assessed by energy-dispersive X-ray spectroscopy (EDX), and magnetic properties were measured using a vibrating sample magnetometer. FTIR spectroscopy was used to evaluate the polymerization of the MNP-laden adhesive. We prepared cavities in molar phantoms divided in four groups (n = 15 each) restored using the same adhesive with different MNP contents: Group 0 (G0)—no MNPs, G1—MNPs-SiO2, G2—MNPs-Ca, and G3—uncoated MNPs. The restoration’s color was quantified in the CIELAB color space using a dental spectrophotometer. Results: MNPs-SiO2 were globular, whereas MNPs-Ca had a cubic morphology. The SiO2 layer was 73.1 nm ± 9.9 nm thick; the Ca(OH)2 layer was 19.97 nm ± 2.27 nm thick. The saturation magnetization was 18.6 emu/g for MNPs-SiO2, 1.0 emu/g for MNPs-Ca, and 65.7 emu/g for uncoated MNPs. MNPs had a marginal effect on the adhesive’s photopolymerization. The mean color difference between G0 and G2 was close to the 50:50% acceptability threshold, whereas the other groups were far apart from G0. The mean whiteness index of G2 did not differ significantly from that of G0; G1 deviated marginally from G0, whereas G3 differed significantly from G0. Conclusions: These results suggest that MNP coating can mitigate the influence of MNP-laden dental adhesives on the color of restorations. Full article
(This article belongs to the Collection New Concepts for Dental Treatments and Evaluations)
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12 pages, 3142 KiB  
Article
The Influence of Drying Time, Application Mode, and Agitation on the Dentin Bond Strength of a Novel Mesoporous Bioactive Glass-Containing Universal Dentin Adhesive
by Jiyoung Kwon, Jungwon Kim, Dongseok Choi and Duck-Su Kim
J. Funct. Biomater. 2025, 16(7), 247; https://doi.org/10.3390/jfb16070247 - 5 Jul 2025
Viewed by 533
Abstract
This study evaluated the influence of drying time, application mode, and agitation on the micro-tensile bond strength (μTBS) of a novel mesoporous bioactive glass-containing universal adhesive (Hi-Bond Universal). Twelve experimental groups were established according to drying time (blot-dry, 10 s dry, or 20 [...] Read more.
This study evaluated the influence of drying time, application mode, and agitation on the micro-tensile bond strength (μTBS) of a novel mesoporous bioactive glass-containing universal adhesive (Hi-Bond Universal). Twelve experimental groups were established according to drying time (blot-dry, 10 s dry, or 20 s dry), application mode (total-etch or self-etch), and agitation (with or without). The μTBS test and failure mode analysis were performed for each experimental group (n = 20), and an adhesive interface was observed using field-emission scanning electron microscopy. The μTBS of all experimental groups was analyzed using a three-way ANOVA and Tukey’s honestly significant difference (HSD) post hoc test (α = 0.05). The total-etch mode yielded higher μTBS than the self-etch mode in the blot-dry and 10 s dry groups (p < 0.05). Agitation also significantly increased the μTBS in the blot-dry and 10 s dry groups for both application modes (p < 0.05). However, application mode and agitation had no effect on the μTBS in the 20 s dry group (p > 0.05). FE-SEM revealed longer and more uniform resin tags after agitation in the blot-dry and 10 s dry groups for both application modes. In conclusion, total-etch mode and agitation effectively increased the bond strength of mesoporous bioactive glass-containing universal adhesives. Full article
(This article belongs to the Special Issue Recent Advancements in Dental Restorative Materials)
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11 pages, 888 KiB  
Article
Evaluation of Four Different Adhesive Systems’ Bonding Strength Between Superficial and Deep Dentin
by Dersim Gökce, Aslihan Usumez, Zelal Seyfioglu Polat and Emrah Ayna
Materials 2025, 18(13), 3107; https://doi.org/10.3390/ma18133107 - 1 Jul 2025
Viewed by 342
Abstract
The success of adhesive restorations largely depends on the optimal bond strength between the tooth structure and the restorative material. The aim of this study was to evaluate the shear bond strength (SBS) of four different adhesives applied to mandibular molars on deep [...] Read more.
The success of adhesive restorations largely depends on the optimal bond strength between the tooth structure and the restorative material. The aim of this study was to evaluate the shear bond strength (SBS) of four different adhesives applied to mandibular molars on deep and superficial dentin. The total of 56 teeth used in the study were randomly divided into 2 subgroups of superficial dentin and deep dentin participants (n = 28). Superficial and deep dentin groups were randomly divided into 4 subgroups (n = 7) for application with different adhesive agents. We formed the following groups: Group 1 (G1)—deep dentin and GC bonding agent (G-Premio BOND); Group 2 (G2)—superficial dentin and GC bonding agent; Group 3 (G3)—deep dentin and Clearfil S3 bond bonding agent (Clearfil TM S3 BOND); Group 4 (G4)—superficial dentin and Clearfil S3 bond bonding agent; Group 5 (G5)—deep dentin and KerrOptibond bonding agent (KerrOptibondTM Universal); Group 6 (G6)—superficial dentin and Kerr Optibond bonding agent; Group 7 (G7)—deep dentin and 3M-ESPE universal bonding agent (3M ESPE); Group 8 (G8)—superficial dentin and 3M-ESPE universal bonding agent. The silicone block with a diameter of 3 mm and a thickness of 1 mm was placed in the middle of the occlusal surface and the test composite was loaded. All prepared specimens were aged in thermal cycles at 5–55 °C for 5000 cycles. The teeth were subjected to SBS (shear bond strength) tests at a crosshead speed of 1 mm/min in a universal testing machine. In all adhesive systems, deep dentin showed a higher bond strength than superficial dentin and the bond strength value was statistically significant (p = 0.05). The bond strength in all tested adhesive systems was observed to be significantly higher in deep dentin than in superficial dentin. Full article
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14 pages, 1955 KiB  
Article
Adhesive Performance of Zirconia and Lithium Disilicate Maryland Cantilever Restorations on Prepared and Non-Prepared Abutment Teeth: An In Vitro Comparative Study
by Tareq Hajaj, Ioana Elena Lile, Radu Marcel Negru, Serban Talpos Niculescu, Sami Stuparu, Mihai Rominu, Cosmin Sinescu, Paul Albu, Florina Titihazan and Ioana Veja
Biomimetics 2025, 10(7), 413; https://doi.org/10.3390/biomimetics10070413 - 21 Jun 2025
Viewed by 441
Abstract
Aim: This in vitro study aimed to evaluate the adhesive performance of zirconia and lithium disilicate Maryland cantilever restorations on prepared and non-prepared anterior abutment teeth. While conventional clinical protocols involve abutment tooth preparation, no-preparation (no-prep) restorations have emerged as a viable, minimally [...] Read more.
Aim: This in vitro study aimed to evaluate the adhesive performance of zirconia and lithium disilicate Maryland cantilever restorations on prepared and non-prepared anterior abutment teeth. While conventional clinical protocols involve abutment tooth preparation, no-preparation (no-prep) restorations have emerged as a viable, minimally invasive alternative. This study compared the adhesion fracture resistance (N) of zirconia restorations on non-prepared enamel surfaces with those on prepared surfaces exposing the dentin. Additionally, the zirconia restorations were compared with lithium disilicate Maryland cantilever restorations, a more common yet costly alternative. Materials and Methods: Forty extracted anterior teeth were allocated into four groups based on preparation type (prepared vs. non-prepared) and material (zirconia vs. lithium disilicate). Each group received cantilevered single-unit FPDs fabricated via CAD/CAM and adhesively cemented using Variolink® Esthetic DC. Standardized loading was applied using a universal testing machine, and the fracture resistance was recorded. Results: The fracture resistance ranged from 190 to 447 N in the zirconia groups and from 219 to 412 N in the lithium disilicate groups. When comparing all the zirconia versus all the lithium disilicate ceramic restorations, regardless of tooth preparation, no statistically significant difference was found (p = 0.752). However, the non-prepared restorations exhibited significantly higher fracture resistance than their prepared counterparts (p = 0.004 for zirconia; p = 0.012 for lithium disilicate ceramic). All the failures were attributed to tooth fracture, except one zirconia restoration, with no debonding observed. Conclusions: Both zirconia and lithium disilicate Maryland cantilever restorations demonstrated reliable adhesive performance when bonded using appropriate surface conditioning and adhesive protocols. Interestingly, the non-prepared designs exhibited higher fracture resistance than the prepared abutments, highlighting their potential advantage in minimally invasive restorative dentistry. Zirconia Maryland bridges, in particular, represent a cost-effective and mechanically resilient option for anterior single-tooth replacement. Full article
(This article belongs to the Special Issue Biomimetic Bonded Restorations for Dental Applications: 2nd Edition)
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13 pages, 3366 KiB  
Article
Compatibility of Dual-Cure Core Materials with Self-Etching Adhesives
by Zachary K. Greene, Augusto A. Robles and Nathaniel C. Lawson
Dent. J. 2025, 13(7), 276; https://doi.org/10.3390/dj13070276 - 20 Jun 2025
Viewed by 343
Abstract
Background/Objectives: A material incompatibility has been established between self-etching adhesives and amine-containing dual-cure resin composite materials used for core buildups. This study aims to compare the dentin bond strength of several amine-containing and amine-free core materials using self-etching adhesives with different pHs. [...] Read more.
Background/Objectives: A material incompatibility has been established between self-etching adhesives and amine-containing dual-cure resin composite materials used for core buildups. This study aims to compare the dentin bond strength of several amine-containing and amine-free core materials using self-etching adhesives with different pHs. Methods: Extracted human molars were mounted in acrylic and ground flat with 320-grit silicon carbide paper. Next, 520 specimens (n = 10/group) were assigned to a dual-cure core buildup material group (10 amine-containing, 2 amine-free, and 1 reference light-cure only bulk fill flowable composite) and assigned to a self-etching adhesive subgroup (pH levels of approximately 1.0, 3.0, and 4.0). Within 4 h of surface preparation, the adhesive corresponding to the specimen’s subgroup was applied and light-cured. Composite buttons for the assigned dual-cure core material of each group were placed using a bonding clamp apparatus, allowed to self-cure for 2 h at 37 °C, and then unclamped. An additional group with one adhesive (pH = 3.0) was prepared in which the dual-cure core materials were light-cured. The bonded specimens were stored in water at 37 °C for 24 h. The specimens were mounted on a testing clamp and de-bonded in a universal testing machine with a load applied to a circular notched-edge blade at a crosshead speed of 1 mm/min until bond failure. The maximum load divided by the area of the button was recorded as the shear bond strength. The data was analyzed via 2-way ANOVA. Results: The analysis of bond strength via 2-way ANOVA determined statistically significant differences between the adhesives, the core materials, and their interaction (p < 0.01). There was a general trend in shear bond strength for the adhesives, where pH 4.0 > 3.0 > 1.0. The amine-free core materials consistently demonstrated higher shear bond strengths as compared to the other core materials when chemically cured only. Light-curing improved bond strength for some materials with perceived incompatibility. Conclusions: The results of this study suggest that an incompatibility can exist between self-etching adhesives and dual-cure resin composite core materials. A decrease in the pH of the utilized adhesive corresponded to a decrease in the bond strength of dual-cure core materials when self-curing. This incompatibility may be minimized with the use of core materials formulated with amine-free chemistry. Alternatively, the dual-cure core materials may be light-cured. Full article
(This article belongs to the Special Issue Dental Materials Design and Innovative Treatment Approach)
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15 pages, 2675 KiB  
Article
Aloe Vera as an Adjunct in Endodontic Irrigation: Impact on Dentin Bond Strength and Cytotoxicity
by Lucas David Galvani, Ester Alves Ferreira Bordini, Diana Gabriela Soares, Joatan Lucas de Sousa Gomes Costa, José Rodolfo Verbicário, Fernando Pozzi Semeghini Guastaldi, Milton Carlos Kuga and Luís Geraldo Vaz
Materials 2025, 18(12), 2874; https://doi.org/10.3390/ma18122874 - 18 Jun 2025
Viewed by 347
Abstract
This study evaluated the effects of mechanical agitation of Aloe vera Barbadensis Miller solution at different concentrations using passive ultrasonic irrigation (PUI), XP Endo Finisher (XPF), XP Clean (XPC), and Easy Clean (ECL), compared to conventional endodontic irrigation (CIE), on bond strength and [...] Read more.
This study evaluated the effects of mechanical agitation of Aloe vera Barbadensis Miller solution at different concentrations using passive ultrasonic irrigation (PUI), XP Endo Finisher (XPF), XP Clean (XPC), and Easy Clean (ECL), compared to conventional endodontic irrigation (CIE), on bond strength and adhesive failure patterns in the cervical, middle, and apical thirds of the root canal. Aloe vera solutions at 1%, 3%, and 5% were tested to reverse collagen fiber collapse induced by hypochlorous acid, a free radical released by 2.5% sodium hypochlorite, which impairs dentin hybridization and the light curing of resin cement. Fiberglass posts were cemented using an etch-and-rinse adhesive system (Ambar; FGM) and conventional dual resin cement (Allcem Core) in root dentin across all thirds. Human teeth underwent chemical–mechanical preparation, and the Aloe vera solution was agitated using the CIE, PUI, XPF, XPC, or ECL protocols. Slices from each root third were evaluated under a stereomicroscope at 10× magnification and subjected to the push-out test. Cytotoxicity was assessed by applying various Aloe vera concentrations to stem cells from the apical papilla (SCAPs) for 24 h, followed by analysis of cell metabolism (Alamar Blue), viability (Live/Dead), and proliferation (F-actin). Aloe vera demonstrated significant biological activity and enhanced bond strength, particularly at 3% and 5%, irrespective of the agitation method or root third. Thus, it can be concluded that using Aloe vera solution is an alternative for pre-treatment before the cementation of fiberglass posts with conventional dual-cure resin cement in endodontically treated dentin. Full article
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17 pages, 3792 KiB  
Article
Influence of Cement Thickness, Dentine Thickness, and Intracoronal Depth on the Fracture Resistance of 3D-Printed Endocrowns: A Pilot In Vitro Study
by Osama Abuabboud, Adrian-George Marinescu, Mihai Paven, Izabella-Maria Kovacs, Luminita Maria Nica, Andrei-Bogdan Faur, Dan Ioan Stoia and Anca Jivănescu
Dent. J. 2025, 13(6), 263; https://doi.org/10.3390/dj13060263 - 12 Jun 2025
Viewed by 956
Abstract
Background/Objectives: Endodontically treated molars are structurally weakened due to internal tissue loss, increasing their risk of fracture. Endocrowns, developed as a conservative alternative to post–core systems, have gained popularity with the rise of digital dentistry, CAD/CAM workflows, and 3D-printed restorations. In this context, [...] Read more.
Background/Objectives: Endodontically treated molars are structurally weakened due to internal tissue loss, increasing their risk of fracture. Endocrowns, developed as a conservative alternative to post–core systems, have gained popularity with the rise of digital dentistry, CAD/CAM workflows, and 3D-printed restorations. In this context, the aim of the present pilot study was to investigate the influence of cement layer thickness, intracoronal depth, and dentine wall thickness on the fracture resistance of molars restored with 3D-printed endocrowns. Methods: Twelve extracted human molars were endodontically treated and restored with endocrowns fabricated from a 3D-printed resin material, SprintRay CrownTM (SprintRay Inc. Los Angeles, CA, USA), via masked stereolithography (MSLA) on a Prusa SL1 printer. Cementation was performed using RelyX Universal Resin Cement (3M, Maplewood, MN USA). Cone beam computed tomography (CBCT) was used to measure the dentine thickness and intracoronal depth before cementation and cement thickness after cementation. The fracture resistance was evaluated using a universal testing machine. For each variable (Td, Dp, Tc), the 12 specimens were divided into two groups (n = 6). Statistical analysis included Pearson correlation, a one-way ANOVA, and the non-parametric Mann–Whitney U test. Results: Within the limitations of this pilot in vitro study, cement thickness demonstrated a strong positive correlation with fracture resistance (r = 0.577) and was the only variable showing statistical significance in the ANOVA (F = 7.847, p = 0.019). In contrast, intracoronal depth and dentine wall thickness exhibited weaker and nonsignificant correlations. No significant mechanical advantage was observed from increasing the pulp chamber depth or peripheral dentine wall thickness. This result was further supported by nonparametric Mann–Whitney U testing (p = 0.015). Conclusions: Cement layer thickness is a key biomechanical factor influencing the fracture resistance of endocrown restorations. Preparation depth and dentine wall geometry appear to have a less direct impact. Full article
(This article belongs to the Special Issue Innovative Materials in Dental Prosthetics)
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24 pages, 34320 KiB  
Case Report
A 10-Year Follow-Up of an Approach to Restore a Case of Extreme Erosive Tooth Wear
by Davide Foschi, Andrea Abate, Francesca Vailati, Ignazio Loi, Cinzia Maspero and Valentina Lanteri
Dent. J. 2025, 13(6), 259; https://doi.org/10.3390/dj13060259 - 10 Jun 2025
Viewed by 1304
Abstract
Background: In recent years, thanks to the improvement of adhesive techniques, patients affected by tooth wear, related to erosion and/or parafunctional habits, can undergo restoration by adding only what has been lost of their dentition (additive approach). However, since not all clinicians are [...] Read more.
Background: In recent years, thanks to the improvement of adhesive techniques, patients affected by tooth wear, related to erosion and/or parafunctional habits, can undergo restoration by adding only what has been lost of their dentition (additive approach). However, since not all clinicians are convinced that dental rehabilitation should be proposed in the early stages of exposed dentin, several treatments are often postponed. It is important to emphasize that, in the early stages, the clinical approach should remain conservative, focusing on dietary counseling, the modification of harmful habits, fluoride application, and risk factor management. Only when these preventive and non-invasive strategies prove insufficient, and the condition continues to progress, should invasive restorative treatments be considered. Unfortunately, epidemiological studies are reporting an increase in the number of young patients affected by erosive tooth wear, and not intercepting these cases earlier could lead to a severe degradation of the affected dentition. In addition, parafunctional habits are also becoming more frequent among patients. The combination of erosion and attrition can be very destructive, and may progress rapidly once dentin is exposed and the risk factors remain unaddressed. The aim of this report was to present a conservative full-mouth rehabilitation approach for severe erosive lesions and to provide a 10-year follow-up assessing the biological, functional, and esthetic outcomes. Methods: In this article, the postponed restorative treatment of a patient, suffering from severe tooth wear, is illustrated. The patient had sought dental treatment in the past; however, due to the already very compromised dentition, a conventional but very aggressive treatment was proposed and refused. Four years later, when the patient finally accepted an alternative conservative therapy, the tooth degradation was very severe, especially at the level of the maxillary anterior teeth. The combination of three different approaches, Speed-Up Therapy, BOPT (Biologically-Oriented Preparation Technique), and the 3 Step Technique, however, improved the capacity to successfully complete the difficult therapeutic task. Results: The biological goals (maintenance of the pulp vitality of all of the teeth and the minimal removal of healthy tooth structure) were accomplished, relying only on adhesive techniques. Conclusions: The overall treatment was very comfortable for the patient and less complicated for the clinician. At 10-year follow-up, biological, functional, and esthetic success was still confirmed. Full article
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19 pages, 1981 KiB  
Article
Impact of a Novel Pretreatment on Bond Strength of Universal Adhesive to Conventional and CAD/CAM Resin Composites: In Vitro Study
by Ali A. Elkaffas, Abdullah Alshehri, Feras Alhalabi, Rania Bayoumi, Abdullah Ali Alqahtani, Abdulellah Almudahi, Abdulaziz Fahd Alsubaie and Abdulaziz Fahd Alharbi
J. Funct. Biomater. 2025, 16(6), 197; https://doi.org/10.3390/jfb16060197 - 27 May 2025
Viewed by 564
Abstract
Novel dentin bonding pretreatment using copper sulfate (CuSO4) and dipotassium hydrogen phosphate (K2HPO4) may create a more hydrophobic environment for dentin bonding. Thus, this study aims to investigate the impact of a CuSO4 + K2 [...] Read more.
Novel dentin bonding pretreatment using copper sulfate (CuSO4) and dipotassium hydrogen phosphate (K2HPO4) may create a more hydrophobic environment for dentin bonding. Thus, this study aims to investigate the impact of a CuSO4 + K2HPO4 pretreatment on dentin μTBS when bonded with a universal adhesive to conventional and CAD/CAM resin composites. Eighty recently extracted human molars (n = 80) were chosen and placed in transparent acrylic blocks to expose the crowns entirely. Nano-filled resin composite and CAD/CAM resin blocks were selected. Based on the dentin pretreatment, type of resin composite, and adhesion strategy, the teeth were randomly allocated into eight equal groups (n = 10). The microtensile bond strength (μTBS) and fracture mode were determined. A three-way analysis of variance (ANOVA) was used to analyze the μTBS data, followed by Tukey’s post hoc test. The μTBS values were not significantly affected by either the resin composite type (p > 0.05) or the adhesive strategy (p > 0.05) according to the three-way ANOVA results. Conversely, significant differences were detected between no dentin pretreatment (24.20 ± 4.54 MPa) and CuSO4 + K2HPO4 pretreatment (33.66 ± 5.22 MPa) using an etch-and-rinse adhesive strategy for nano-filled composites (p < 0.001). Additionally, significant differences were detected between no dentin pretreatment (24.71 ± 4.33 MPa) and CuSO4 + K2HPO4 pretreatment (32.49 ± 4.92 MPa) using an etch-and-rinse adhesive strategy for CAD/CAM resin blocks (p < 0.001). Moreover, significant differences were detected between no dentin pretreatment (21.20 ± 3.40 MPa) and CuSO4 + K2HPO4 pretreatment (30.31 ± 3.87 MPa) using a self-etching adhesive strategy for nano-filled composites (p < 0.001). Also, significant differences were detected between no dentin pretreatment (23.89 ± 3.89 MPa) and CuSO4 + K2HPO4 pretreatment (31.22 ± 4.71 MPa) using a self-etching adhesive strategy for CAD/CAM resin blocks (p < 0.001). In conclusion, dentin μTBS was enhanced by a copper-based treatment when used with nano-filled and CAD/CAM resin blocks. Full article
(This article belongs to the Special Issue Advances in Restorative Dentistry Materials)
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23 pages, 2027 KiB  
Article
Development and Evaluation of a Novel Self-Etch Dental Adhesive Incorporating Graphene Oxide–Zirconia (GO-ZrO2) and Hydroxyapatite–Zinc (HA-Zn) for Enhanced Bond Strength, Biocompatibility, and Long-Term Stability
by Norbert Erich Serfözö, Marioara Moldovan, Doina Prodan and Nicoleta Ilie
Nanomaterials 2025, 15(11), 803; https://doi.org/10.3390/nano15110803 - 27 May 2025
Viewed by 476
Abstract
The aim of this study was to develop an experimental self-etch dental adhesive (SE) by synthesizing graphene oxide–functionalized zirconia (GO-ZrO2) and hydroxyapatite–functionalized zinc (HA-Zn) as inorganic powders together with bis-GMA (0–2) (bisphenol A-glycidyl methacrylate) oligomers as main components of the organic [...] Read more.
The aim of this study was to develop an experimental self-etch dental adhesive (SE) by synthesizing graphene oxide–functionalized zirconia (GO-ZrO2) and hydroxyapatite–functionalized zinc (HA-Zn) as inorganic powders together with bis-GMA (0–2) (bisphenol A-glycidyl methacrylate) oligomers as main components of the organic matrix. The adhesive was compared to the current gold standard adhesive Clearfill SE Bond 2 (CSE) using cytotoxicity assays, shear bond strength (SBS) tests, and resin–dentin interface analyses. Cytotoxicity assays with human gingival fibroblasts (HGF-1) revealed reduced cell viability at early time points but indicated favourable biocompatibility and potential cell proliferation at later stages. SBS values for the experimental adhesive were comparable to CSE after 24 h of storage while aging did not significantly affect its bond strength. However, SBS exhibited more consistent resin tag formation and higher Weibull modulus values post-aging. A scanning electron microscopy (SEM) analysis highlighted differences in resin tag formation, suggesting the experimental adhesive relies more on chemical bonding than micromechanical interaction. The experimental adhesive demonstrated promising potential clinical properties and bond durability due to the integration of GO-ZrO2 and HA-Zn fillers into the adhesive. Full article
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17 pages, 1276 KiB  
Review
Deep Caries Lesions Revisited: A Narrative Review
by Irina Maria Gheorghiu, Sergiu Ciobanu, Ion Roman, Stana Păunică, Anca Silvia Dumitriu and Alexandru Andrei Iliescu
J. Mind Med. Sci. 2025, 12(1), 37; https://doi.org/10.3390/jmms12010037 - 23 May 2025
Viewed by 1961
Abstract
Background/Objectives: Deep caries lesions represent an actual concern in preserving tooth vitality and preventing irreversible pulpitis. As presently the non-selective approach is considered an overtreatment, the concept of selective caries removal is highly recommended. The goal of this narrative review is to [...] Read more.
Background/Objectives: Deep caries lesions represent an actual concern in preserving tooth vitality and preventing irreversible pulpitis. As presently the non-selective approach is considered an overtreatment, the concept of selective caries removal is highly recommended. The goal of this narrative review is to focus on current trends in carious dentine excavation and adjunctive therapies. Methods: A keyword-based selection of scientific publications issued in the last six years, i.e., 2019–2024, was conducted with the search engine of PubMed (MEDLINE) and ScienceDirect databases, using the following keywords: deep carious lesion; caries removal; indirect pulp capping; adjunctive antimicrobial therapy; adjunctive anti-enzymatic therapy; biomimetic restorative dentistry. Discussions: In deep caries management, the current trends of carious dentine excavation recommend preferentially partial caries removal technique as less risky to pulp exposure and more conservative compared to the stepwise technique (SW). Presently, advanced additional procedures such as antimicrobial photodynamic therapy and an anti-enzymatic approach are also considered for caries arrest. Conclusions: Selective caries removal and adjunctive photodynamic antimicrobial therapy are procedures of choice in preserving pulp vitality. Anti-enzymatic therapies impede decoupling with time of adhesive restorations from the smear layer. Biomimetic restorative dentistry and smart materials introduce the principles of artificial intelligence in the therapeutic approach of deep caries. Full article
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10 pages, 1752 KiB  
Article
Influence of Different Fiber-Reinforced Biobases on the Marginal Adaptation of Lithium Disilicate Overlay Restorations (A Comparative In Vitro Study)
by Maareb Abdulraheem Nabat and Alaa Jawad Kadhim
Prosthesis 2025, 7(3), 55; https://doi.org/10.3390/prosthesis7030055 - 22 May 2025
Viewed by 1083
Abstract
Background/purpose: Fiber-reinforced materials are commonly used as biobases beneath indirect restorations, potentially affecting the seating and marginal accuracy of the restorations. This study intended to assess the impact of various biobase techniques on the marginal adaptation of lithium disilicate overlay restorations. Methods [...] Read more.
Background/purpose: Fiber-reinforced materials are commonly used as biobases beneath indirect restorations, potentially affecting the seating and marginal accuracy of the restorations. This study intended to assess the impact of various biobase techniques on the marginal adaptation of lithium disilicate overlay restorations. Methods: Fifty sound maxillary first premolar teeth of comparable dimensions were prepared using a full-bevel overlay design (3 mm occlusal reduction) and allocated randomly to five groups as follows (n = 10): Group A, delayed dentin sealing; Group B, immediate dentin sealing using Optibond FL; Group C, immediate dentin sealing with a 1 mm flowable composite layer (Clearfil AP-X Flow); Group D, immediate dentin sealing followed by a 1 mm short-fiber-reinforced composite layer (everX Flow); and Group E, immediate dentin sealing coated with a 1 mm flowable composite layer reinforced with polyethylene Ribbond fibers. Digital impressions were obtained using a Medit i700 intraoral scanner, and the overlays were digitally designed via the Sirona inLab CAD software and milled via a four-axis milling machine. The overlays were luted with a preheated composite (Clearfil AP-X). Marginal gap assessments were conducted pre- and post-cementation via a digital microscope at 230× magnification. The data were statistically analyzed using a one-way analysis of variance and paired t-tests. Results: The one-way ANOVA disclosed no significant differences among the groups before or after cementation (p > 0.05). Conclusions: The presence or absence of fiber-reinforced biobases did not impact the marginal adaptation of the restorations; these biobases can be incorporated to optimize the mechanical behavior of indirect restorations without adversely affecting their seating accuracy. These findings suggest that fiber-reinforced and non-reinforced biobase techniques can be safely integrated into clinical adhesive protocols to enhance the mechanical performance of restorations without comprising their marginal adaptation. Full article
(This article belongs to the Section Prosthodontics)
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18 pages, 2832 KiB  
Article
Effects of Grape Seed Extract-Modified Etchants on Collagenolytic Activity, Interface Formation, and Bonding Longevity of Adhesive–Dentin Interfaces
by Viviane Hass, Xiaomei Yao and Yong Wang
Materials 2025, 18(11), 2416; https://doi.org/10.3390/ma18112416 - 22 May 2025
Viewed by 499
Abstract
This study investigated the effects of acid etching with grape seed extract (GSE)-modified etchants, varying phosphoric acid (PA) concentrations, on endogenous collagenolytic activity of etched dentin, adhesive–dentin (A/D) interfacial formation, and bond strength over time. Three PA concentrations (5%, 10%, and 20%) were [...] Read more.
This study investigated the effects of acid etching with grape seed extract (GSE)-modified etchants, varying phosphoric acid (PA) concentrations, on endogenous collagenolytic activity of etched dentin, adhesive–dentin (A/D) interfacial formation, and bond strength over time. Three PA concentrations (5%, 10%, and 20%) were combined with 2% GSE (5PA/GSE, 10PA/GSE, and 20PA/GSE) and compared to a control (CT) group using 32% PA gel (3M Universal Scotchbond etchant). Seventy-four caries-free human third molars were sectioned to expose dentin surfaces, which were etched and analyzed. In situ zymography with confocal laser microscopy was used to assess endogenous collagenolytic activity in etched dentin specimens. For A/D interfacial morphology and bond strength, etched dentin was bonded with Adper Single Bond Plus adhesive (3M ESPE) and composite buildup. The interfacial morphology of A/D specimens was evaluated using either Goldner’s trichrome staining under light microscopy after microtomy sectioning or scanning electron microscopy. A/D specimens were stored in either TESCA buffer or collagenase solution and tested immediately (IM) or at multiple time points over one year using the microtensile bond strength (μTBS) test. Data were analyzed by one- or three-way ANOVA followed by Games–Howell or Tukey’s tests (α = 0.05). GSE-modified etchants significantly reduced endogenous collagenolytic activity (p < 0.05). Although GSE-modified etchants resulted in thinner A/D interfaces, the bond strength remained unaffected (p > 0.05). Bond strength stability was prolonged up to one year with 5PA/GSE and 10PA/GSE (p < 0.001), while CT or 20PA/GSE showed significant degradation by 17 weeks (p < 0.01). Storage in the more aggressive collagenase solution did not further reduce the bond strength compared to TESCA buffer (p = 0.966). Acid etching with GSE-modified etchants effectively inhibits endogenous MMP-mediated collagenolytic activity. At 5% and 10% PA, this approach enhances the stability of the A/D bond strength, offering a promising modification for dentin bonding protocols. Full article
(This article belongs to the Special Issue Advanced Materials for Oral Applications)
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14 pages, 6740 KiB  
Article
Er:YAG Laser in QSP Modality for Treatment of Indirect Adhesive Restoration Build-Up: Surface Roughness Analysis and Morphology Assessment by Environmental Scanning Electron Microscopy (ESEM)
by Ilaria Giovannacci, Monica Mattarozzi, Fabrizio Moroni, Giuseppe Pedrazzi, Paolo Vescovi and Maria Careri
Dent. J. 2025, 13(5), 223; https://doi.org/10.3390/dj13050223 - 21 May 2025
Viewed by 383
Abstract
Background/Objectives: Sandblasting build-ups before applying the acid and adhesive significantly improves the bond strength. The aim of this study is to evaluate, for the first time, the effectiveness of an Er:YAG laser used in QSP mode to treat the surface of build-ups before [...] Read more.
Background/Objectives: Sandblasting build-ups before applying the acid and adhesive significantly improves the bond strength. The aim of this study is to evaluate, for the first time, the effectiveness of an Er:YAG laser used in QSP mode to treat the surface of build-ups before the adhesive cementation sequence. Methods: This ex vivo study was conducted on 12 intact, undecayed extracted teeth kept hydrated in NaCl 0.9% solution. A cavity was created in the center and reconstructed with composite resin (build-up). Then, samples were prepared with burs and divided into three groups: control group G1, prepared only with burs; group G2, in which surfaces were treated with a sandblaster (2.5 bar, 10 mm from composite surface, aluminum oxide, 10 s); and group G3, treated using an Er:YAG laser (QSP modality, 1 W, 10 Hz, 100 mJ). The surface roughness of the build-ups was measured using a CCI MP-L digital optical profiler (Taylor Hobson, Leicester, UK), and surface morphology was studied using the Quanta™ 250 FEG (FEI, Hillsboro, OR, USA) ESEM instrument. Results: Regarding enamel, mean surface roughness in G1 vs. G2 was not statistically significant (p = 0.968); meanwhile, differences between the Er:YAG laser group (G3) and G1 or G2 were significant (G3 vs. G1 p < 0.001; G3 vs. G2 p < 0.001). Regarding dentin, G1 vs. G2 was significant (p = 0.021); differences between G3 and G1 or G2 were extremely significant (G3 vs. G1 p < 0.001; G3 vs. G2 p < 0.001). The same trend was detected for resin. Conclusions: An Er:YAG laser in QSP mode used on the build-up surface for indirect adhesive restorations is innovative and should be investigated with further studies. However, it seems extremely effective with increased roughness, the absence of a smear layer and characteristics potentially favorable for good adhesion for all substrates (enamel, dentin, resin). Full article
(This article belongs to the Special Issue Laser Dentistry: The Current Status and Developments)
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9 pages, 736 KiB  
Article
Effect of Two Different Adhesion Modes of a Universal Resin Cement on the Retention of Glass Fiber Posts Cemented to Root Canal Dentine: An In Vitro Study
by Rani D’haese, Valentin Vervack, Inas Hamid and Stefan Vandeweghe
Adhesives 2025, 1(2), 8; https://doi.org/10.3390/adhesives1020008 - 21 May 2025
Viewed by 479
Abstract
Purpose: The aim of this in vitro study was to investigate the adhesive bond strength of glass fiber posts when cemented with universal resin cement in two different adhesion modes: adhesive and self-adhesive. Methods: A total of 20 extracted single-root teeth were endodontically [...] Read more.
Purpose: The aim of this in vitro study was to investigate the adhesive bond strength of glass fiber posts when cemented with universal resin cement in two different adhesion modes: adhesive and self-adhesive. Methods: A total of 20 extracted single-root teeth were endodontically treated, decoronated and prepared to receive glass fiber posts (GFPs) with a diameter of 1.6 mm (RelyX fiber post 3D). Specimens were randomly divided into two groups: (G1) GFPs were cemented using RelyX Universal cement in self-adhesive mode, and (G2) GFPs were cemented using Scotch Bond Universal Plus and RelyX Universal cement (adhesive mode). Afterwards, the specimens were sliced at three root levels: coronal, middle and apical. Bond strength was measured using a push-out test. Data were analyzed with a two-way analysis of variance (ANOVA) test and independent sample T-test. Results: Bond strength was significantly influenced by the adhesive strategy (p < 0.025) and the position of the root third (p < 0.007). Microscopic analysis of failure mode revealed a higher prevalence of adhesive failures (cement–dentine). Conclusions: Glass fiber posts cemented with universal resin cement applied in adhesive mode showed significantly higher push-out bond strength than when applied in self-adhesive mode. In both study groups, the apical root regions exhibited the highest retention values, followed by the middle and coronal regions. Full article
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