Search Results (34)

This study examined the distribution and seasonal population dynamics of tick species infesting cattle, sheep, and goats in Ağrı Province, Türkiye. From January to December 2024, 913 hosts were examined, and 545 intact tick samples were collected from 386 infested animals and morphologically identified. The overall sex ratio was 52.7% female and 47.3% male. The identified species included Dermacentor niveus, D. marginatus, Rhipicephalus sanguineus, R. turanicus, R. bursa, Haemaphysalis punctata, Hae. sulcata, Hyalomma marginatum, H. anatolicum anatolicum, H. anatolicum excavatum, and H. detritum detritum. Seasonal tick activity peaked in summer (51.7%) and spring (42.9%), with a significant decline in autumn (χ² = 48.72, df = 3, p < 0.001). No active ticks were observed in winter due to the harsh continental climate. Adult Hyalomma ticks, which are known potential vectors of Crimean–Congo hemorrhagic fever (CCHF), remained active except during winter. The high prevalence of these vector species suggests a need for further investigation into pathogen circulation in the region. This research provides a scientific foundation for integrated vector control and surveillance programs within the One Health framework. Full article

The increasing abundance of ticks poses a growing public health concern due to heightened human exposure to tick bites. The lone star tick (Amblyomma americanum: Ixodida: Ixodidae), a common human-biting species in the United States, has expanded its range in recent years. However, how its different life stages vary across time, habitats, and locations remains insufficiently understood. We analyzed tick abundance data collected in southeastern Virginia between 2009 and 2018, focusing on larval, nymphal, and adult life stages. A Bayesian multivariate modelling framework was used to examine seasonal patterns, habitat effects, spatial variation, and biological links between life stages. Two commonly used count models were compared to determine which best described the observed tick abundance patterns. Tick abundance showed strong and distinct seasonal patterns across life stages. Adult ticks were most abundant in late spring to early summer (May–June), nymphs peaked in early to mid-summer (June–July), and larvae peaked later in summer (August). Wooded habitats consistently supported higher tick abundance than grassy areas. Although both models captured these trends, the negative binomial model provided a more stable and biologically meaningful representation of tick dynamics. Several counties, including Chesapeake, York, Portsmouth, and Northampton, were identified as areas of elevated tick abundance, indicating increased tick bite exposure risk. This study highlights clear seasonal and habitat-specific windows of increased tick activity that are relevant for surveillance and control planning. By clarifying when and where different tick life stages are more abundant, the findings support targeted public health interventions aimed at reducing human exposure to tick bites in Virginia. The modelling approach is also applicable to other regions, including settings where ticks affect livestock health and food security. Full article

Tick-borne diseases are a growing public health concern in the Mediterranean. Brijuni National Park (BNP), a unique, highly visited island ecosystem characterized by increased large game host density and diverse Mediterranean habitats, presents an elevated risk for pathogen co-circulation. This study addresses the lack of spatial and epidemiological data to accurately assess human exposure risk in this environment. We performed a detailed geospatial and epidemiological risk mapping of pathogen co-circulation in BNP. A total of 587 hard ticks were collected across 26 georeferenced micro-locations (2020–2022). Ticks were morphologically identified and subsequently screened for six key zoonotic bacterial pathogens using qPCR. The Minimal Infection Rate (MIR) and a Co-infection Rate (CR) were calculated. Geographic Information System (GIS) mapping was utilized to map ecological determinants of risk. Ixodes ricinus was the overwhelmingly dominant vector (94.0%), peaking in spring, with activity absent in summer. Recorded diverse tick fauna also included Hyalomma marginatum (3%), Haemaphysalis punctata (2%), Ixodes frontalis (0.8%) and Rhipicephalus sanguineus (0.2%). Active circulation of Borrelia burgdorferi s.l. (Bbsl), Anaplasma phagocytophilum, and Ehrlichia canis were confirmed. Bbsl presented the highest MIR (3.05). The Co-infection Rate (CR) was notably high at 29.41%, with triple co-infections (Bbsl, A. phagocytophilum, E. canis) concentrated in cultivated mosaics and holm oak forests (Quercus ilex L.). The highest number of ticks was recovered from ecotone zones, accounting for 50.0% of the total catch, confirming them as high-risk interfaces. The absence of Rickettsia conorii may be attributed to the strict control/absence of its primary host (domestic dogs). The presence of the exotic vector H. marginatum was also confirmed. The high rate of co-infection and the spatial concentration of risk in specific habitats underscore an elevated and complex public health risk in BNP, closely linked to habitat structure and increased game host density. This research provides an essential geospatial framework for targeted ‘One Health’ management, prioritizing vector control in ecotone zones and dense forest refugia. Urgent surveillance for the exotic H. marginatum is warranted to monitor the potential risk of Crimean-Congo Hemorrhagic Fever. Full article

Tick-borne diseases pose a significant threat to global cattle production, with species displacement between ticks compounding this issue. This narrative review synthesises the literature to examine the drivers behind the expansion of the invasive Rhipicephalus microplus and its displacement of the native Rhipicephalus decoloratus in Southern Africa. We analysed the biological, ecological, environmental, and anthropogenic factors by reviewing existing scientific studies and reports. Our findings indicate that R. microplus possesses a competitive advantage due to its shorter life cycle, higher reproductive output, and greater acaricide resistance. Furthermore, anthropogenic activities such as communal grazing practices, unregulated livestock movement, and land-use changes facilitate the spread of this parasite. Climate change and vegetation shifts also create more favourable habitats for this invasive species. The conclusion is that the displacement of R. decoloratus by R. microplus intensifies the burden of tick-borne diseases, leading to substantial economic losses. Effective mitigation requires an integrated tick management approach that combines chemical, biological, and ecological strategies, supported by improved surveillance and farmer education. Full article

Despite serological evidence of tick-borne encephalitis virus (TBEV) circulation in Belgian animals since 2007, confirmed autochthonous human infection was only first documented in 2020. We review the current national epidemiologic situation and investigate a household cluster of confirmed autochthonous cases identified in 2025. A cohabiting couple experienced a near-simultaneous onset of meningoencephalitis and tested positive for TBEV-specific IgM and IgG, with confirmation by PRNT90. One patient reported a recent tick bite, and both patients reported consumption of unpasteurized milk and goat cheese, suggesting possible alimentary transmission. The identification of Case 2, who lacked neurological symptoms at presentation and was only tested due to the index case, illustrates the risk of missed diagnoses and supports the notion that human TBEV infection is likely underdiagnosed in Belgium. These findings underscore the need to increase clinical awareness, strengthen surveillance, and reinforce prevention strategies. TBE should be considered in the differential diagnosis of patients presenting with non-specific fever or neurological syndromes such as meningoencephalitis, particularly during the spring-to-autumn tick activity season. Full article

The Carpathian Basin is a coherent biogeographic unit whose wildlife populations and pathogen dynamics are increasingly reshaped by administratively fragmented governance, land-use change and linear infrastructure. This review synthesizes evidence that the permeability patterns governing host movement also structure the transboundary exchange of genes and infections, creating a connectivity substrate for conservation genetics and One Health risk. Focusing on wild boar (Sus scrofa), red deer (Cervus elaphus), roe deer (Capreolus capreolus), fallow deer (Dama dama) and the expanding golden jackal (Canis aureus), we integrate population genetic inferences with wildlife epidemiology to examine how highways, border fences and asymmetric management (e.g., supplemental, feeding practices, hunting pressure and surveillance regimes) can generate biological asymmetries across boundaries. We highlight African swine fever as an emblematic disturbance in wild boar populations, discuss cervid risks including tick-borne pathogens and chronic wasting disease (CWD) preparedness and evaluate zoonotic threats associated with carnivore expansion (e.g., Echinococcus spp.). We propose a Carpathian Basin-level monitoring and data-sharing architecture, coupling harmonized passive surveillance, strategic active surveillance for priority pathogens, and standardized genetic marker panels supported by interoperable metadata. A Basin-scale One Health approach is a pragmatic prerequisite for the coordinated prevention, early detection and resilient management of cross-border epizootics and zoonotic threats. Full article

Jingmen tick virus (JMTV) is a novel flavi-like virus first identified in 2010 in Rhipicephalus microplus in the Jingmen region of Hubei Province, China and has been reported in different Asian countries, Central and South America, Africa, and Europe. Beyond ticks, JMTV has been detected in a range of other arthropods and in vertebrate hosts. In humans, JMTV has been found in patients with Crimean-Congo hemorrhagic fever in Kosovo and Turkey, and in febrile patients with a history of tick bites in China, suggesting it may be a novel human pathogen. To investigate the presence of JMTV in Italy, we developed a One-step real-time RT-PCR assay and applied it to individually screen 1150 ticks collected from northeastern, central, and southern Italy. JMTV RNA was detected in multiple tick species, including Ixodes ricinus, Rhipicephalus bursa, Rhipicephalus sanguineus s.l., Dermacentor marginatus, and Hyalomma marginatum with a prevalence ranging from 0.52% to 18.42% in questing ticks. The detection of JMTV in ticks from all surveyed areas, indicates that the virus is geographically widespread in Italy. These findings highlight the need for comprehensive surveillance strategies to identify new areas of active virus circulation and to investigate the potential impact of JMTV on public health. Full article

Pig farms in Southern Nigeria face recurrent threats from enzootic viral infections, yet active surveillance remains limited. This study implemented an active surveillance approach targeting African swine fever virus (ASFV) to assess its circulation across four states. We sampled 40 pig farms and two abattoirs, collecting swine blood and ticks for molecular and serological analysis. Sampling was conducted during both African swine fever (ASF) outbreak (n = 27 pigs) and non-outbreak (n = 204 pigs) periods, resulting in 231 samples tested for ASFV DNA. Additionally, 46 plasma samples from the non-outbreak period were screened for ASFV-specific antibodies. ASFV was detected in all pigs sampled during outbreak periods (27/27; 100%), whereas none of the pigs sampled during non-outbreak periods tested positive (0/204). Of the 27 positives, sequencing generated 7 genomes characterized as genotype II. Serological testing of the 46 plasma samples from non-outbreak pigs revealed no ASFV seropositivity. All tick samples were negative for ASFV DNA. Farm-level risk evaluations revealed widespread biosecurity lapses, suggesting these deficiencies as potential drivers of ASFV circulation. These findings underscore the urgent need to strengthen farm-level biosecurity and demonstrate the pivotal role of active surveillance and genome sequencing in detecting and characterizing ASF outbreaks. Full article

Background: Disruptions in the mesh of the ecosystem come with implications that severely harm the sustainability and the equilibrium of life. Interactions of humans, animals, and many other organisms, along with the whole ecological complex, have given birth to zoonotic diseases, which can vary in type and burden. Collaborative efforts put into the prioritization of environmental, animal, and human health are envisioned as “One Health”. Understanding vector ecology and the varying mechanistic ways of transmission is crucial for constructing effective One Health surveillance tools and warning systems. Methods: We identified the literature available concerning the subject matter. We utilized scholarly databases to gather research for the last 10 years using predefined keywords. Objectives: This review aims to synthesize current knowledge on the interconnection between climate discrepancies, ecological alarms, and the emergence and spread of zoonotic diseases. We attempted to provide recommendations for future research and policy interventions. Results: Human activities have significantly impacted disease-carrying vectors and wildlife habitats, aiding their proliferation and the spillover of diseases. Global frameworks incorporating One Health principles enhance global preparedness for future health threats. Applying the integrated One Health Surveillance has strengthened early warning systems. Interdisciplinary collaborations and tools like OH-EpiCap, a comprehensive tool that assesses and enhances the capacities of One Health surveillance systems, have significantly contributed to responding to infectious disease outbreaks, as seen in the Netherlands, reducing the risk of tick-borne diseases. Conclusions: Strides have been made with comprehensive processes that identify and prioritize zoonotic diseases of most significant concern and burden, such as OHZDP, approaches like One Health, and other theories considered. A proactive and integrated approach will build resilience against potential outbreaks and ensure a healthier future for our planet and its inhabitants. Full article

Ticks are key vectors of zoonotic pathogens, and their expanding distribution in Europe heightens public health concerns. In Bosnia and Herzegovina, while tick distribution is well documented, molecular data on tick-borne pathogens remain limited. This study aimed to illustrate the presence and diversity of these pathogens, focusing on areas with high human activity. Ticks (n = 556) were collected in April 2022 from eight diverse locations, including urban parks, private properties, and rural sites. PCR-based screening was employed to detect Anaplasmataceae, Borrelia, Francisella, Piroplasmida, Rickettsia, and tick-borne encephalitis virus (TBEV), with subsequent sequencing to confirm results. Further characterization of Borrelia burgdorferi sensu lato was achieved via reverse line blotting (RLB) hybridization and sequencing. Ixodes ricinus was the most prevalent species, followed by Dermacentor marginatus and D. reticulatus. Our analysis revealed an overall infection rate of 22.1% in questing ticks, with Rickettsia spp. and Borrelia spp. predominating. Notably, seven Borrelia species were identified in I. ricinus, alongside Anaplasma phagocytophilum, Rickettsia helvetica, and R. monacensis, with co-infections mainly observed in peri-urban areas. This study provides the first molecular evidence of multiple tick-borne pathogens in the region, underscoring the need for further surveillance and risk assessment of tick-borne diseases in the region. Full article

Rapid environmental changes driven by human activities are contributing to a significant decline in global biodiversity, with avian species being particularly affected due to their migratory behavior. As highly mobile hosts, birds facilitate the geographic dispersal of ectoparasites, including ticks, which serve as vectors for numerous zoonotic pathogens. This study, conducted in collaboration with the Faunistic Observatory of the Asinara National Park between 2021 and 2023, aimed to investigate the potential role of migratory birds in tick dispersal and the presence of Borrelia spp. DNA. Birds were captured using mist nets during pre-breeding (April–May) and post-breeding (October–November) migration periods. Ticks were systematically collected and identified at the species level, and molecular analyses were performed using real-time and conventional PCR to detect the presence of Borrelia spp. DNA. Results showed a distinct seasonal variation in tick species composition. In autumn, Ixodes ricinus was predominant (99%), whereas Hyalomma species were more frequently observed in spring (78%). Molecular screening revealed Borrelia spp. DNA in 26.1% of the collected ticks, with Borrelia garinii being the most prevalent species. These findings underscore the ecological significance of migratory birds in the dissemination of ticks and tick-borne pathogens, highlighting their potential role in shaping disease transmission dynamics across different geographic regions. This study provides valuable insights into the seasonal fluctuations in tick populations associated with migratory avifauna and the epidemiological risks posed by these interactions. Continued surveillance of migratory birds as vectors of zoonotic pathogens is essential for informing public health strategies and mitigating the risks of emerging infectious diseases, but further investigation is needed to clarify the actual role of migratory birds in the transmission of Borrelia spp. Full article

Active and passive surveillance, followed by gene sequencing, continue to be used to identify a diverse range of novel bacteria, viruses, and other microorganisms in ticks with the potential to cause disease in vertebrate hosts following tick bite. In this study, we describe the isolation and characterization of a novel virus from Bothriocroton hydrosauri ticks collected from a blotched blue-tongue, Tiliqua nigrolutea. In an attempt to isolate rickettsia, the inoculation of Vero cell cultures with tick extracts led to the isolation of a virus, identified as a novel tick Orthomyxovirus by electron microscopy and gene sequencing. Transmission electron microscopic analysis revealed that B. hydrosauri tick virus-1 (BHTV-1) is a spherical orthomyxovirus, 85 nm in size. Multiple developmental stages of the virus were evident in vitro. Analysis of putative BHTV-1 amino acid sequences derived from a genomic analysis of virus-infected host cell extracts revealed the presence of six putative RNA segments encoding genes, sharing the closest sequence similarity to viral sequences belonging to the arthropod-borne Thogotovirus genus within the Orthomyxoviridae. Thogotoviruses are an emerging cause of disease in humans and animals following tick bite. The detection of this new thogotovirus, BHTV-1, in B. hydrosauri, a competent vector for human tick-borne infectious diseases, warrants follow-up investigation to determine its prevalence, host range, and pathogenic potential. Full article

Climate changes and human-related activities are identified as major factors responsible for the increasing distribution and abundance of vectors worldwide and, consequently, of vector-borne diseases (VBDs). Farmed animals, during grazing or in establishments with the absence of biosecurity measures, can easily be exposed to wildlife showing high-risk of contagion of several infectious diseases, including VBDs. Furthermore, livestock represents an interface between wildlife and humans, and thus, promoting the transmission pathway of VBDs. Little is known about the presence and prevalence of VBDs in livestock in Southern Italy; therefore, the present study evaluated the circulation of zoonotic VBDs in livestock and potential risk of exposure. A total of 621 whole blood samples belonging to cattle and buffaloes (n = 345) and small ruminants (n = 276) were examined by molecular examinations for the detection of tick-borne pathogens (TBPs). High prevalence (66.3%) for at least one agent was observed. Moreover, the risk of exposure related to environmental features was assessed, as follows: presence of humid areas, high-density of animals, and sample collection during May. These results show a high circulation of TBPs among livestock and underline the need for surveillance in high-risk habitats for public health. Full article

Lyme borreliosis (LB) is not notifiable in many European countries, and the patchwork of surveillance strategies in Europe perpetuates knowledge gaps. In the Netherlands, LB incidence has been estimated from recurring general practitioner surveys since the 1990s. To complement the incidence data, this study aimed to estimate the prevalence of antibodies against Borrelia burgdorferi sensu lato in the general population of the Netherlands in 1995/1996, identify risk factors for seropositivity, and compare these findings to data from 2016/2017 to identify temporal trends. Sera from participants (n = 8041, aged 0–80 years) in a cross-sectional nationwide surveillance study were assessed for the presence of antibodies against B. burgdorferi s.l., using a screening ELISA and immunoblot confirmation. Risk factors associated with seropositivity were evaluated using multivariable analysis. A significant difference in weighted seroprevalence was observed between 1995/1996 (2.8%) and 2016/2017 (4.3%). In both cohorts, the seroprevalence was significantly higher among men than among women, and increased with age and tick bite frequency. The upward trend in age-specific seropositivity in individuals over 50 was steeper in 2016/2017 than in 1995/1996, possibly due to improved fitness among contemporary elderly, allowing increased outdoor activities. This study highlights significant trends in the seroprevalence of B. burgdorferi s.l. antibodies in the general population of the Netherlands over 20 years. The doubling of seroprevalence underscores the increasing burden of LB, and the importance of continued surveillance. Targeted interventions, particularly for elderly populations, may help raise awareness to the risks of tick bites and reduce the growing disease burden and societal costs associated with LB. Full article

In the northeastern USA, the distribution of lone star ticks (Amblyomma americanum) has expanded northward in recent decades, overlapping with the range of blacklegged ticks (Ixodes scapularis). Blacklegged ticks carry pathogens for diseases such as Lyme, babesiosis, and anaplasmosis, while bites from lone star ticks cause other diseases and the alpha-gal syndrome allergy. Lone star ticks can become so abundant that they are perceived as more of a public health threat than blacklegged ticks. Using the island of Martha’s Vineyard, Massachusetts, as a case study, we analyzed data from a total of 1265 yard surveys from 2011 to 2024 to document lone star tick presence and subsequent expansion from two peripheral areas, Chappaquiddick and Aquinnah, to all six towns. The timing of lone star tick expansion on Martha’s Vineyard closely matched an increase in tick submissions to a pathogen testing center. At Chappaquiddick, drag sampling carried out in June 2023 and 2024 showed that both tick species were most common at wooded sites, where blacklegged nymphs were somewhat more abundant than lone star nymphs. However, lone star ticks occurred in a wider range of natural and peridomestic habitats than blacklegged nymphs, making them far more challenging for people to avoid and manage. Full article