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18 pages, 3711 KB  
Article
Urban Villages as Hotspots of Road-Deposited Sediment: Implications for Sustainable Urban Management
by Mengnan He, Cheng Chen, Jianmin Zhang, Jinge Ma and Yang Liu
Sustainability 2026, 18(3), 1543; https://doi.org/10.3390/su18031543 - 3 Feb 2026
Abstract
Rapid urbanization has fostered the proliferation of urban villages (UVs), high-density informal settlements that pose unique challenges for environmental management. Despite their prevalence, the dynamics of pollutant accumulation in these transitional neighborhoods remain underexplored. This study investigated nitrogen and phosphorus accumulation in road-deposited [...] Read more.
Rapid urbanization has fostered the proliferation of urban villages (UVs), high-density informal settlements that pose unique challenges for environmental management. Despite their prevalence, the dynamics of pollutant accumulation in these transitional neighborhoods remain underexplored. This study investigated nitrogen and phosphorus accumulation in road-deposited sediment (RDS) within Shenzhen, a representative megacity in southern China, utilizing field sampling and statistical analysis to identify dominant drivers. The results indicate that UVs function as significant pollution hotspots, with RDS accumulation rates approximately 3.7 times higher than in formal built-up areas. Analysis revealed that pollution intensity is primarily driven by natural factors such as slope, whereas pollution load is controlled by anthropogenic supply factors. This creates a critical input–output imbalance where high pollutant inputs exceed the natural removal capacity. Consequently, effective mitigation of urban non-point source pollution requires shifting from traditional engineering solutions to spatially sensitive planning strategies, offering practical guidance for enhancing urban sustainability in rapidly urbanizing regions. Full article
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18 pages, 4846 KB  
Article
Microplastics in Pristine Caves of the Classic Karst (NE Italy): A First Assessment of Contamination Levels
by Raffaele Bruschi, Manuela Piccardo, Tecla Bentivoglio, Serena Anselmi, Patrice Cabanel, Stanislao Bevilacqua, Lucia Gardossi and Monia Renzi
Microplastics 2026, 5(1), 24; https://doi.org/10.3390/microplastics5010024 - 3 Feb 2026
Abstract
Data on microplastic contamination in pristine caves are rare, thus limiting our understanding of its pervasiveness in intact underground ecosystems. Here, we quantified microplastics in sediments from two newly discovered, extremely remote caves (Maucci and Luftloch) and compared them with a frequently visited [...] Read more.
Data on microplastic contamination in pristine caves are rare, thus limiting our understanding of its pervasiveness in intact underground ecosystems. Here, we quantified microplastics in sediments from two newly discovered, extremely remote caves (Maucci and Luftloch) and compared them with a frequently visited cave (Trebiciano), all three of which are hydraulically connected to the Reka/Timavo River in the Classic Karst (NE Italy). Sediment samples were collected along river-to-slope transects and analyzed for microplastics using density separation and μFT-IR spectroscopy. Average contamination levels were comparable across caves, ranging from 84.7 to 105.9 items kg−1 (dry weight). Fibers and fragments dominated, with similar polymer spectra across sites—polypropylene (PP, 29–42%), polyethylene (PE, 19–27%), and polyethylene terephthalate (PET, 33–46%). Microplastic abundance systematically increased with elevation, up to ~4–12× from river-proximal to high-bench sediments. Polymer-resolved trends reflected density-coupled, flood-driven sorting with low-density PP and PE accumulated on higher benches and denser PET depleted aloft, indicating slackwater retention at flood crests and re-entrainment of lower benches during recession. These findings suggest that indirect riverine inputs of microplastics outweigh direct human contamination and provide the first baseline for pristine Timavo caves—serving as reference sites for background microplastic levels in the Classic Karst and similar karst systems worldwide. Full article
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26 pages, 8290 KB  
Article
Modeling and Factor Assessment of Pond Silting in Forest-Steppe Agrolandscapes of the Central Russian Upland
by Natalya A. Skokova, Anastasiya G. Narozhnyaya, Artyom V. Gusarov and Fedor N. Lisetskii
Geographies 2026, 6(1), 13; https://doi.org/10.3390/geographies6010013 - 1 Feb 2026
Viewed by 44
Abstract
This paper presents the results of assessing the influence of siltation factors in 23 ponds in one of the most agriculturally developed macro-regions of European Russia—the Central Russian Upland. Key natural and anthropogenic factors determining the intensity of pond siltation have been identified, [...] Read more.
This paper presents the results of assessing the influence of siltation factors in 23 ponds in one of the most agriculturally developed macro-regions of European Russia—the Central Russian Upland. Key natural and anthropogenic factors determining the intensity of pond siltation have been identified, and a typification of ponds has been developed to predict the rate of accumulation of bottom sediments in them. For the typification, statistical methods such as correlation analysis (Spearman’s coefficient), cluster and factor analysis, and the Random Forest machine learning algorithm were used. Correlation analysis revealed that the percentage of catchment cultivation has a significant effect (r = 0.55, p < 0.01) on the volume of bottom sediments, while soil loss (r = 0.47, p < 0.05) and vertical terrain dissection (r = 0.43, p < 0.05) have a moderate effect. The most important factors in the siltation process are the average slope of the catchment (24.5%), the percentage of cultivated soils (18.8%), and the average annual soil loss (14.1%). All factors were grouped into three clusters, which explained 77.8% of the variance. As a result, four pond types were identified, differing in their dominant limiting factors: pond hydrological characteristics, catchment morphometry, and the degree of anthropogenic transformation of the catchment. Verification of the typification was carried out based on the calculation of annual soil losses considering the sediment delivery coefficient; the discrepancies between the calculated and actual pond sediment volumes were 1.2–10.0%. The proposed approach, which recommends a multi-scale assessment of potential sediment formation volumes using remote sensing data and thematic mapping, offers heuristic potential for identifying the most degraded water bodies. This enables the planning of priority sites and rehabilitation measures for their restoration within the framework of regional soil and water conservation programs. Full article
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13 pages, 1715 KB  
Article
Effects of Long-Term Mulching on Soil Aggregation and Organic Carbon Sequestration in Sloping Croplands of the Loess Plateau
by Xicheng Cao, Zhiguo Yang, Guangxin Ren, Gaihe Yang, Na Yang, Ke Wang, Jian Wang, Xing Wang, Jiajie Song, Jiancheng Zhang and Yongzhong Feng
Agriculture 2026, 16(3), 294; https://doi.org/10.3390/agriculture16030294 - 23 Jan 2026
Viewed by 266
Abstract
Sloping cropland on the Loess Plateau faces severe challenges from soil organic carbon (SOC) depletion and structural instability due to erosion and intensive tillage. Although mulching can enhance SOC sequestration, its long-term effects on the spatial distribution of SOC and aggregates across slopes [...] Read more.
Sloping cropland on the Loess Plateau faces severe challenges from soil organic carbon (SOC) depletion and structural instability due to erosion and intensive tillage. Although mulching can enhance SOC sequestration, its long-term effects on the spatial distribution of SOC and aggregates across slopes remain unclear. A 15-year field experiment evaluated five practices—conventional tillage (T), no tillage (NT), straw mulching (SM), plastic film mulching (PM), and ridge–furrow plastic film mulching (RPM)—on SOC storage, aggregate stability, and their variation with different slope positions. Compared to T, all mulching treatments significantly increased SOC concentration by 4.19% to 83.48% in the 0–30 cm layer. SM and RPM notably increased macro-aggregates (>2 mm) and their associated SOC (24.04–56.49% higher than T) by adding organic matter and optimizing micro-topography. Different slope positions strongly influenced SOC redistribution: lower slopes accumulated more SOC than upper slopes due to erosion–deposition processes. Mulching reduced SOC spatial variability and minimized differences between slope positions. Although mulching increased cumulative SOC mineralization compared to T, the long-term net SOC gain was positive, driven by improved aggregate protection and reduced erosion. SM and RPM are recommended for sustainable slope farmland management due to their dual benefits in enhancing carbon sinks and soil stability. This study offers practical strategies for improving soil health and SOC sequestration in vulnerable sloping landscapes. Full article
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24 pages, 4689 KB  
Article
Development of a Thermo-Mechanical Model for PVC Geomembrane—Application to Geomembrane Stability on Dam Slopes
by Hamza Tahir, Guillaume Veylon, Guillaume Stoltz and Laurent Peyras
Appl. Sci. 2026, 16(3), 1160; https://doi.org/10.3390/app16031160 - 23 Jan 2026
Viewed by 157
Abstract
The mechanical response of geomembranes in hydraulic structures is strongly influenced by temperature variations, which alter both material stiffness and interface shear strength behavior. This study develops a non-linear, temperature-dependent tensile behavior constitutive model for a polyvinyl chloride (PVC) geomembrane and evaluates its [...] Read more.
The mechanical response of geomembranes in hydraulic structures is strongly influenced by temperature variations, which alter both material stiffness and interface shear strength behavior. This study develops a non-linear, temperature-dependent tensile behavior constitutive model for a polyvinyl chloride (PVC) geomembrane and evaluates its implications for the stability of geomembrane-lined reservoir slopes. The empirical relationship was calibrated using tensile tests reported in literature for temperatures between 10 °C and 60 °C, reproducing the observed non-linear softening and modulus reduction with increasing temperature. A classical thermal dilation formulation was incorporated to simulate cyclic thermal expansion and contraction. The constitutive and thermal formulations were implemented in FLAC2D and applied to a 2H:1V covered geomembrane slope representative of dam lining systems. The results show that temperature-induced softening significantly increases tensile strain within the geomembrane. The model also shows that the lower surface interface friction angle of the geomembrane plays a significant role in the slope stability. Thermal cycle analysis demonstrates the accumulation of efforts resulting from the fatigue of the geomembrane. The proposed model provides a practical framework for incorporating thermo-mechanical coupling in design analyses and highlights the necessity of accounting for realistic thermal conditions in assessing the long-term stability of geomembrane-lined reservoirs. Full article
(This article belongs to the Section Civil Engineering)
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20 pages, 20102 KB  
Article
Influence of Alpine Forest Types on Soil Microbial Diversity and Soil Quality
by Shuang Ji, Xunxun Qiu, Huichun Xie, Zhiqiang Dong and Hongye Li
Plants 2026, 15(2), 315; https://doi.org/10.3390/plants15020315 - 21 Jan 2026
Viewed by 186
Abstract
Alpine forests are key regulators of soil biogeochemical cycles, yet the extent to which forest type constrains soil microbial diversity and soil quality in high-elevation regions remains insufficiently resolved. Here, we assessed how contrasting alpine forest types influence the taxonomic composition and diversity [...] Read more.
Alpine forests are key regulators of soil biogeochemical cycles, yet the extent to which forest type constrains soil microbial diversity and soil quality in high-elevation regions remains insufficiently resolved. Here, we assessed how contrasting alpine forest types influence the taxonomic composition and diversity of soil microbial communities, identified the dominant environmental drivers, and evaluated soil quality along the southern slope of the Qilian Mountains. Six forest types were examined, including four monospecific stands (Picea crassifolia, QQ; Betula spp., HS; Juniperus przewalskii, YB; and Pinus tabuliformis, YS) and two mixed formations (mixed conifer–broadleaf, ZKHJ; and mixed broadleaved, KKHJ). Bacterial and fungal communities were characterized using Illumina high-throughput sequencing, while structural equation modeling (SEM) was used to identify primary drivers of diversity and principal component analysis (PCA) was applied to construct the minimum data set (MDS) for soil quality evaluation. Mixed forests consistently exhibited higher bacterial and fungal alpha diversity than pure stands. Environmental gradients were the strongest positive drivers of microbial diversity, whereas soil chemical properties and vegetation-related biotic factors exerted partially negative effects. Soil quality index (SQI) values ranked as follows: KKHJ (0.55) > ZKHJ (0.49) > YB (0.48) > HS (0.46) > YS (0.44) > QQ (0.43). The mixed broadleaved forest reached Grade IV (upper-intermediate level) soil quality, whereas the other forest types were classified as Grade III (intermediate). Mixed forests showed stronger capacities for organic matter accumulation and nutrient retention. These findings indicate that promoting mixed forest stands is critical for improving soil structure, nutrient retention, and microbial diversity in this alpine region. Accordingly, forest management should prioritize the development of mixed forests to enhance overall soil quality. Full article
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21 pages, 20951 KB  
Article
Study of the Mining Depth of Tailings Considering the Stability of Existing Open-Pit Slopes
by Haiyu Ji, Chong Li, Xinfeng Yang, Yanchang Li, Shaodong Li and Shuzhao Feng
Appl. Sci. 2026, 16(2), 577; https://doi.org/10.3390/app16020577 - 6 Jan 2026
Viewed by 238
Abstract
The recovery and comprehensive utilization of tailings resources can effectively mitigate or eliminate safety hazards in the upper zones of open-pit mines. To ensure the safe recovery of accumulated tailings and enhance resource utilization efficiency, this study establishes a two-dimensional model based on [...] Read more.
The recovery and comprehensive utilization of tailings resources can effectively mitigate or eliminate safety hazards in the upper zones of open-pit mines. To ensure the safe recovery of accumulated tailings and enhance resource utilization efficiency, this study establishes a two-dimensional model based on the Discrete Element Method (DEM) for the overall stability of tailings recovery, which is integrated with the existing slope and ore pillar models of the open-pit mine. Leveraging the mechanical parameters of tailings and waste rock obtained from laboratory tests, this study systematically investigates the effects of tailings recovery on the stability of existing slopes. Results show that due to differences in fracture characteristics and tailings reserves, complete tailings extraction causes no landslides in some sections, but large-scale or varying landslides occur on southern/northern flank slopes in specific sections at certain excavation depths or after full extraction. Targeted recovery recommendations are proposed: “segmented excavation with bench reservation” prevents overall landslides on southern flank slopes of landslide-prone sections; 35° slope cutting ensures stability of northern flank slopes in all sections. Further field verification considering rainfall and seismic loading factors is required for practical applications. Full article
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14 pages, 2253 KB  
Article
Comparison of Particle Number Concentrations Between Small and Large Urban Green Spaces During a PM Pollution Episode in Seoul, South Korea
by Sumin Choi, Taehee Kim and Chan-Ryul Park
Land 2026, 15(1), 103; https://doi.org/10.3390/land15010103 - 5 Jan 2026
Viewed by 348
Abstract
We analyzed the effect of reducing particulate matter in a forest by comparing concentrations and particle number concentrations (PNCs) between urban and background forest areas with the use of aerodynamic particle sizers. PM was observed at forest and urban sites during the high [...] Read more.
We analyzed the effect of reducing particulate matter in a forest by comparing concentrations and particle number concentrations (PNCs) between urban and background forest areas with the use of aerodynamic particle sizers. PM was observed at forest and urban sites during the high particulate matter events from 22 to 30 April 2019. Comparing the PM concentrations measured, PM10 and PM2.5 were 61.6 μg/m3 and 36.9 μg/m3, respectively, in the urban site, while PM10 and PM2.5 were 53.9 μg/m3 and 31.8 μg/m3, respectively, in the forest site. Most PNCs at both sites ranged in particle size from less than 0.5 μm (99%). During high-concentration events, the mass concentration of PM10 was not significantly different, but PNCs of the accumulation mode particles (≤0.5 µm) and coarse mode particles (>0.5 µm) were differed between two sites. The re-duction rate of coarse mode particles (>0.5 µm) was lower 20% at large urban green space. A large urban green space showed the high slope value of decrease at the relationship between aerodynamic diameter and PNC at all times. These results indicate that not only mass concentration but PNC could support to understand the PM traits at large urban green space during the PM pollution of episode. Full article
(This article belongs to the Special Issue Healthy and Inclusive Urban Public Spaces)
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31 pages, 5378 KB  
Article
Composite Fractal Index for Assessing Voltage Resilience in RES-Dominated Smart Distribution Networks
by Plamen Stanchev and Nikolay Hinov
Fractal Fract. 2026, 10(1), 32; https://doi.org/10.3390/fractalfract10010032 - 5 Jan 2026
Viewed by 189
Abstract
This work presents a lightweight and interpretable framework for the early warning of voltage stability degradation in distribution networks, based on fractal and spectral features from flow measurements. We propose a Fast Voltage Stability Index (FVSI), which combines four independent indicators: the Detrended [...] Read more.
This work presents a lightweight and interpretable framework for the early warning of voltage stability degradation in distribution networks, based on fractal and spectral features from flow measurements. We propose a Fast Voltage Stability Index (FVSI), which combines four independent indicators: the Detrended Fluctuation Analysis (DFA) exponent α (a proxy for long-term correlation), the width of the multifractal spectrum Δα, the slope of the spectral density β in the low-frequency range, and the c2 curvature of multiscale structure functions. The indicators are calculated in sliding windows on per-node series of voltage in per unit Vpu and reactive power Q, standardized against an adaptive rolling/first-N baseline, and anomalies over time are accumulated using the Exponentially Weighted Moving Average (EWMA) and Cumulative SUM (CUSUM). A full online pipeline is implemented with robust preprocessing, automatic scaling, thresholding, and visualizations at the system level with an overview and heat maps and at the node level and panel graphs. Based on the standard IEEE 13-node scheme, we demonstrate that the Fractal Voltage Stability Index (FVSI_Fr) responds sensitively before reaching limit states by increasing α, widening Δα, a more negative c2, and increasing β, locating the most vulnerable nodes and intervals. The approach is of low computational complexity, robust to noise and gaps, and compatible with real-time Phasor Measurement Unit (PMU)/Supervisory Control and Data Acquisition (SCADA) streams. The results suggest that FVSI_Fr is a useful operational signal for preventive actions (Q-support, load management/Photovoltaic System (PV)). Future work includes the calibration of weights and thresholds based on data and validation based on long field series. Full article
(This article belongs to the Special Issue Fractional-Order Dynamics and Control in Green Energy Systems)
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30 pages, 15497 KB  
Article
Geological and Social Factors Related to Disasters Caused by Complex Mass Movements: The Quilloturo Landslide in Ecuador (2024)
by Liliana Troncoso, Francisco Javier Torrijo Echarri, Luis Pilatasig, Elías Ibadango, Alex Mateus, Olegario Alonso-Pandavenes, Adans Bermeo, Francisco Javier Robayo and Louis Jost
GeoHazards 2026, 7(1), 4; https://doi.org/10.3390/geohazards7010004 - 1 Jan 2026
Viewed by 453
Abstract
Complex landslides have characteristics and parameters that are difficult to analyze. The landslide on 16 June 2024 in the rural community of Quilloturo (Tungurahua, Ecuador) caused severe damage (14 deaths, 24 injuries, and hundreds of affected families) related to the area’s geological, social, [...] Read more.
Complex landslides have characteristics and parameters that are difficult to analyze. The landslide on 16 June 2024 in the rural community of Quilloturo (Tungurahua, Ecuador) caused severe damage (14 deaths, 24 injuries, and hundreds of affected families) related to the area’s geological, social, and anthropogenic conditions. Its location in the eastern foothills of Ecuador’s Cordillera Real exacerbated the effects of a landslide involving various processes (mud and debris flows, landslides, and rock falls). This event was preceded by intense rainfall lasting more than 10 h, which accumulated and caused natural damming of the streams prior to the event. The lithology of the investigated area includes deformed metamorphic and intrusive rocks overlain by superficial clayey colluvial deposits. The relationship between the geological structures found, such as fractures, joints, schistosity, and shear, favored the formation of blocks within the flow, making mass movement more complex. Geomorphologically, the area features a relief with steep slopes, where ancient landslides or material movements, composed of these colluvial deposits, have already occurred. At the foot of these steep slopes, on plains less than 300 m wide and bordered by the Pastaza River, there are human settlements with less than 60 years of emplacement and a complex history of territorial occupation, characterized by a lack of planning and organization. The memory of the inhabitants identified mass movements that have occurred since the mid-20th century, with the highest frequency of occurrence recorded in the last decade of the present century (2018, 2022, and 2024). Furthermore, it was possible to identify several factors within the knowledge of the inhabitants that can be considered premonitory of a mass movement, specifically a flood, and that must be incorporated as critical elements in decision-making, both individual and collective, for the evacuation of the area. Full article
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27 pages, 9753 KB  
Article
Identification of Potential Flood-Prone Areas in the Republic of Kosovo Using GIS-Based Multi-Criteria Decision-Making and the Analytical Hierarchy Process
by Bashkim Idrizi, Agon Nimani and Lyubka Pashova
Sustainability 2026, 18(1), 359; https://doi.org/10.3390/su18010359 - 30 Dec 2025
Viewed by 439
Abstract
Floods rank among the most frequent and destructive natural hazards, threatening ecosystems, human settlements, and national economies. This study delineates flood-prone areas across Kosovo by developing a national-scale Flood Risk Database (FRDB) and a comprehensive mapping framework integrating Geographic Information Systems (GIS), Multi-Criteria [...] Read more.
Floods rank among the most frequent and destructive natural hazards, threatening ecosystems, human settlements, and national economies. This study delineates flood-prone areas across Kosovo by developing a national-scale Flood Risk Database (FRDB) and a comprehensive mapping framework integrating Geographic Information Systems (GIS), Multi-Criteria Decision-Making (MCDM), and the Analytical Hierarchy Process (AHP). Eight hydrological and topographic conditioning factors—slope, elevation, flow accumulation, distance to rivers, land use/land cover, soil type, precipitation, and drainage density—were analyzed. AHP was employed to assign factor weights based on their relative influence on flood susceptibility, while MCDM aggregated these weighted spatial layers to generate a national flood risk map. Model validation, based on historical flood points, achieved an AUC of 0.909, confirming its high predictive accuracy. The resulting flood risk map classifies Kosovo’s territory into five risk levels: very high (0.56%), high (14.44%), moderate (36.68%), low (46.46%), and very low (1.88%). This research provides the first systematic national-scale FRDB for Kosovo, offering a reliable scientific basis for flood management, spatial planning, and climate resilience policy. Full article
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18 pages, 3970 KB  
Article
Numerical Simulation of the Mineralization Process of the Axi Low-Sulfidation Epithermal Gold Deposit, Western Tianshan, China: Implications for Mineral Exploration
by Wenfa Shan, Xiancheng Mao, Zhankun Liu, Hao Deng, Qiao Yuan and Zhaohui Fu
Minerals 2026, 16(1), 41; https://doi.org/10.3390/min16010041 - 29 Dec 2025
Viewed by 313
Abstract
The Axi gold deposit, a low-sulfidation epithermal deposit in the Western Tianshan, China, hosts over 50 t of gold resources and is widely regarded as the result of coupled processes of rock deformation, heat transfer, pore fluid flow, and chemical reactions. However, research [...] Read more.
The Axi gold deposit, a low-sulfidation epithermal deposit in the Western Tianshan, China, hosts over 50 t of gold resources and is widely regarded as the result of coupled processes of rock deformation, heat transfer, pore fluid flow, and chemical reactions. However, research on the ore-forming processes of this gold deposit from a coupled perspective remains limited, resulting in its ore-forming mechanisms being incompletely understood. In this paper, we use the concept of mineralization rate based on computational modeling to indicate the 3D spatial distribution of mineralization. The simulation results reveal the following: (1) temperature gradients play a key role in influencing mineral precipitation, whereas the effect of pore fluid pressure gradients is relatively negligible; (2) gold precipitation, characterized by a negative mineralization rate, predominantly took place along fault zones that exhibit vertical transitions from steep to gentle slopes or lateral bends, which are further distinguished by the accumulation of fluids and the presence of significant temperature gradients. Notably, this particular distribution pattern of gold precipitation closely mirrors the spatial arrangement of known gold orebodies. These findings suggest that the coupling of multiple physical and chemical processes at specific fault sites plays a critical role in ore formation, providing new insights into the mechanisms governing the development of the Axi gold deposit. Furthermore, based on these observations, it can be inferred that the deeper regions of the Axi gold deposit hold considerable mineralization potential. Full article
(This article belongs to the Special Issue 3D Mineral Prospectivity Modeling Applied to Mineral Deposits)
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11 pages, 3315 KB  
Article
Evaluation of a Cell-Based Potency Assay for Detection of the Potency of TrenibotulinumtoxinE® (TrenibotE)
by Yingchao Yang, Huajie Zhang, Shuo Wang, Yanhua Xue and Liyong Yuan
Toxins 2026, 18(1), 19; https://doi.org/10.3390/toxins18010019 - 29 Dec 2025
Viewed by 451
Abstract
(1) Background: As an innovative drug derived from botulinum neurotoxin serotype E, TrenibotulinumtoxinE® demonstrates a rapid onset and shorter effect. Due to concerns regarding specificity, test throughput, and animal welfare, a new cell-based potency assay (CBPA) method was developed for BoNT/E drug [...] Read more.
(1) Background: As an innovative drug derived from botulinum neurotoxin serotype E, TrenibotulinumtoxinE® demonstrates a rapid onset and shorter effect. Due to concerns regarding specificity, test throughput, and animal welfare, a new cell-based potency assay (CBPA) method was developed for BoNT/E drug substance and drug product; independent evaluation of this new CBPA was required. (2) Methods: The CBPA for BoNT/E is a quantitative assay that measures the accumulated cleaved SNAP25180 in human neuroblastoma cells. It involves sequential culturing, differentiation of cells, and then treatment with drug products. Data were analyzed using a quadratic parallel model via statistical software. Linearity was determined using five effective concentration levels. Key assay parameters including accuracy, linearity, repeatability, intermediate precision and range were evaluated. (3) Results: The overall assay’s accuracy was 98%, and the intermediate precision was 6.3%. The coefficient of determination (R2) and slope were determined as 0.963 and 0.942, respectively. The root mean squared error (RMSE) was 0.057, and the intercept was 0.032 for the combined data. The repeatability was 2.4%, which is well within the acceptance criterion of ≤8%. (4) Conclusions: The evaluation was carried out within a single laboratory under controlled conditions; the new CBPA meets all acceptance criteria and can be used for BoNT/E potency determination. Full article
(This article belongs to the Section Bacterial Toxins)
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16 pages, 63480 KB  
Article
Tectono-Geothermal Coupling Effects on Paleogeomorphology and Source-to-Sink System Evolution of Steep Slope Zones in Lufeng Sag, Pearl River Mouth Basin, South China Sea
by Lihao Bian, Weilin Zhu, Xin Huang, Kai Zhong, Shijie Zhao and Zengyuan Zhou
Appl. Sci. 2026, 16(1), 258; https://doi.org/10.3390/app16010258 - 26 Dec 2025
Viewed by 186
Abstract
As a significant hydrocarbon-bearing sag in the Zhu I Depression of the Pearl River Mouth Basin, the Lufeng sag has long encountered exploration challenges owing to the dearth of comprehensive investigations on the source-to-sink system of the steep slope zone. To tackle this [...] Read more.
As a significant hydrocarbon-bearing sag in the Zhu I Depression of the Pearl River Mouth Basin, the Lufeng sag has long encountered exploration challenges owing to the dearth of comprehensive investigations on the source-to-sink system of the steep slope zone. To tackle this issue, this research utilizes paleogeomorphic reconstruction techniques to conduct a quantitative source-to-sink analysis and establish a hydrocarbon accumulation pattern in the steep slope zone of ductile deformation zones. The findings indicate that the longitudinal drainage systems developed during the intense rifting stage determine the proximal sedimentation. During the minor rifting stage, the area of the lacustrine basin contracted, and the pre-existing drainage systems converged into axial drainage systems. By the late rifting stage, the extent of the drainage system expands significantly, displaying distal sediment source characteristics. The tectono-geothermal regime governs the spatial scale of the source-to-sink system. Quantitative analysis reveals that the denudation and depositional areas in the ductile deformation zones notably exceed those in the brittle deformation zones, thereby creating favorable conditions for the large-scale development of sand bodies and hydrocarbon accumulation in the steep slope zone. These insights offer crucial guidance for future exploration in the steep slope zones of the fault basin. Full article
(This article belongs to the Section Earth Sciences)
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22 pages, 2558 KB  
Article
Post-Fire Restauration in Mediterranean Watersheds: Coupling WiMMed Modeling with LiDAR–Landsat Vegetation Recovery
by Edward A. Velasco Pereira and Rafael Mª Navarro Cerrillo
Remote Sens. 2026, 18(1), 26; https://doi.org/10.3390/rs18010026 - 22 Dec 2025
Viewed by 575
Abstract
Wildfires are among the most severe disturbances in Mediterranean ecosystems, altering vegetation structure, soil properties, and hydrological functioning. Understanding post-fire hydrological dynamics is crucial for predicting flood and erosion risks and vegetation restoration in fire-prone regions. This study investigates the hydrological responses of [...] Read more.
Wildfires are among the most severe disturbances in Mediterranean ecosystems, altering vegetation structure, soil properties, and hydrological functioning. Understanding post-fire hydrological dynamics is crucial for predicting flood and erosion risks and vegetation restoration in fire-prone regions. This study investigates the hydrological responses of Mediterranean watersheds following a wildfire event by integrating WiMMed (Watershed Integrated Management in Mediterranean Environments), a distributed, physically based hydrological model, with high-resolution vegetation data derived from LiDAR and Landsat imagery. A Priority Post-Fire Restoration Index (PPRI) was calculated as the weighted sum of the six parameters runoff (mm), flow accumulation (mm), distance to drainage network (m), slope (%), erodibility (K), lithology, and LiDAR index under a sediment reduction and runoff peak reduction scenario. The post-fire hydrological processes modeled with WiMMed described the dynamics of surface runoff and soil moisture redistribution across the upper soil layers after fire, and their gradual attenuation with vegetation regrowth. The spatial distribution of the PPRI identified specific zones within the burned watershed that require urgent restoration measures (10% and 4.55% under sediment reduction and peak reduction scenarios, respectively). The combined use of process-based modeling and remote sensing offers valuable insights into watershed-scale hydrological resilience and supports the design of post-fire restoration strategies in Mediterranean landscapes. Full article
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