Search Results (2,317)

The sustainable valorization of marine biowaste, particularly shrimp residues, has emerged as a promising strategy to develop eco-friendly agricultural inputs that enhance crop productivity and reduce environmental impacts. This study investigated the effects of a biotechnologically processed fermented shrimp-waste (Parapenaeus longirostris) formulation as a biostimulant on the growth, physiological performance, and development of a local mays variety (Zea mays L., DKC 744) under controlled pot conditions. The experiment evaluated root, foliar, and combined applications of the biostimulant at three concentrations (5%, 10%, and 15%) over a 90-day vegetative cycle. Morphological parameters, including stem height, leaf number, leaf mass, and root biomass, were measured at regular intervals, while chlorophyll a and b contents were assessed to evaluate photosynthetic efficiency. The results indicated that all biostimulant treatments significantly enhanced mays growth. Root-applied biostimulants primarily stimulated root biomass by up to 764.0 ± 66.8 g at the 10% concentration, whereas foliar applications improved above-ground traits, including stem elongation and leaf formation, reaching maximum heights of 200.0 ± 1.9 cm and 17.0 ± 0.4 leaves under intermediate concentrations. Combined root and foliar applications produced the highest stem height (240.0 ± 5.6 cm), leaf number (19.0 ± 0.0), leaf mass (1034.0 ± 11.1 g), and chlorophyll content (2.44 ± 0.9 for chlorophyll a) at 10–15% concentrations. The results also revealed that moderate concentrations generally provided the most balanced stimulation, suggesting the presence of an optimal dose threshold. This study demonstrated the comparative effectiveness of root, foliar, and combined applications of a fermented shrimp-waste biostimulant and identified an optimal concentration. However, its limitations lie in the use of controlled pot conditions and a single crop variety, which restrict the extrapolation of results to field-scale applications and diverse agroecological environments. Therefore, more research is needed to explore the action mechanisms of the studied biostimulant and elicitors, mainly the interaction between biocompounds and the treated plant. Full article

Summer cover crops can improve soil fertility and contribute to nitrogen (N) supply in temperate cropping systems, yet the effects of mixture composition and sowing timing remain insufficiently documented. This study evaluated biomass production and N accumulation of five multispecies cover crop mixtures grown in Estonia during 2024–2025 under two sowing dates per year. Aboveground biomass, botanical composition, and carbon (C) and nitrogen concentrations were measured to assess productivity, species contributions, and residue quality. Earlier sowing was generally associated with higher biomass and N accumulation, with first-sown mixtures producing, on average, 38.7% more biomass than later-sown mixtures. Mixture performance was strongly shaped by species composition and competitive hierarchies. Total N accumulation of the cover crop mixtures ranged from 42 to 275 kg N ha⁻¹ depending on mixture composition and sowing time, with mixtures dominated by common vetch (Vicia sativa L.) achieving the highest values. Oat (Avena sativa) dominated and contributed substantially to biomass in mixtures lacking competitive legumes, whereas sunflower (Helianthus annuus) and maize (Zea mays) performed less well under delayed sowing. Low-growing species such as Persian clover (Trifolium resupinatum) produced little biomass when grown with highly competitive species. Legumes exhibited lower C:N ratios than non-legumes, while mixture-level values remained moderate, suggesting residue quality with potential for favourable decomposition and nutrient release in summer cover crop systems under temperate conditions. Full article

Nitrogen use efficiency (NUE) is a major determinant of maize (Zea mays L.) productivity and sustainability, yet the regulatory changes associated with modern breeding remain incompletely understood. Here, we used breeding-era transcriptomic data from 137 elite Chinese maize inbred lines to identify transcriptional regulators associated with maize NUE. Breeding-era expression shifts in NUE effector genes were modest but tissue-specific, pointing to pathway-level transcriptional rewiring during modern breeding. Focusing on the first leaf above the uppermost ear at silking, we identified 69 breeding-era-responsive genes, including 10 transcription factors, and prioritized ZmSPL19 through Pearson correlation analysis with curated NUE-related genes. ZmSPL19 expression declined during modern breeding and showed a nitrate-repressed expression, with lower transcript abundance under nitrogen-sufficient conditions and rapid downregulation upon nitrate resupply. Loss of ZmSPL19 function promoted primary root elongation, biomass accumulation, leaf nitrogen content, soil–plant analysis development (SPAD), photosynthetic rate, kernel number, and grain yield under nitrogen-sufficient conditions. These results identify ZmSPL19 as a breeding-associated negative regulator of growth and yield formation under nitrogen-sufficient conditions and support the value of a breeding-informed strategy for discovering regulators with potential relevance to maize NUE improvement. Full article

Maize is one of the most widely cultivated crops worldwide and is extensively used for animal feed and industrial applications. Plant height (PH) and ear height (EH) are critical determinants of lodging resistance and tolerance to high planting density, and coordinated regulation of these traits is essential for yield improvement. In this study, 479 maize inbred lines from Northeast and North China were genotyped using 7861 single-nucleotide polymorphism (SNP) markers to perform a genome-wide association study (GWAS). After controlling for population structure and relatedness, the mixed linear model (MLM) identified 20 loci significantly associated with PH on chromosomes 2, 4, 5, 6, 7, and 8, and 8 loci associated with EH on chromosomes 2, 3, 4, and 7. A total of 23 candidate genes were identified, including PLATZ8, pectin methylesterase 36, and leucine-rich repeat extensin 14. Gene Ontology (GO) enrichment analysis revealed significant enrichment in biological and molecular functions such as DNA binding, pectinesterase activity, zinc ion binding, ATP binding, and uniporter activity. Bioinformatic characterization of the two most likely candidate genes, Zm00001d002726 and Zm00001d015394, showed that both possess a typical compact four-exon structure. Functional prediction indicated that Zm00001d002726 encodes a pectinesterase/pectinase, potentially regulating cell elongation through pectin degradation and remodeling of the cell wall. Pectinesterase activity may influence PH and EH by mediating pectin demethylation within the cell wall. In contrast, Zm00001d015394 encodes a PLATZ family transcription factor that may regulate downstream gene expression through DNA-binding activity. These findings provide insight into the genetic architecture and potential molecular mechanisms underlying PH and EH in maize and offer a foundation for future breeding efforts. Full article

Zearalenone (ZEA) is a mycotoxin widely present in cereals, feeds, and foods, posing a persistent threat to human and animal health. Hepatic fibrosis is a pathological process characterized by excessive extracellular matrix (ECM) deposition. Chronic liver injury caused by sustained oxidative stress can initiate the development of early hepatic fibrosis. However, whether liver injury induced by ZEA can trigger hepatic stellate cell (HSC) activation and promote early profibrotic responses remains unclear. The aim of this study was to assess whether ZEA-induced liver injury promotes HSC activation and early profibrotic responses. To address this, we established a BALB/c mouse exposure model and used the murine HSC line (JS-1) for in vitro validation. The results showed that ZEA exposure caused structural damage in hepatic tissue and produced an incomplete bridging pattern of collagen thickening suggestive of an early profibrotic tendency. ZEA shaped a proinflammatory microenvironment by activating the IκBα/NF-κB axis and induced the TGF-β1/Smad2/3 pathway, accompanied by Smad7 suppression, thereby promoting HSC activation and the expression of fibrosis-related genes. ZEA also altered autophagy-related markers in liver tissue and JS-1 cells. Pharmacological inhibition with chloroquine partially attenuated ZEA-induced upregulation of α-SMA and collagen I/III, suggesting that autophagy-related processes may be involved in ZEA-associated HSC activation and early ECM deposition. In summary, ZEA promotes HSC activation and early profibrotic changes in the liver and is associated with inflammatory activation, TGF-β1/Smad signaling, and altered autophagy-related activity. These findings provide a basis for further investigation into the mechanisms underlying ZEA-induced early profibrotic remodeling in the liver. Full article

Brown spot on the leaf sheath is an emerging disease of sweet corn (Zea mays L.) in Sinaloa, Mexico, with an unknown etiology. This study aimed to identify the causal agent of the disease and assess its pathogenicity on commercial sweet corn hybrids. Bacterial strains were isolated from symptomatic leaf sheaths collected from commercial fields. Identification was performed through biochemical profiling (API 50CHB/E), pathogenicity tests on alternative hosts (potato, onion, celery), and molecular analysis (16S rRNA and recA genes sequencing and phylogenetic reconstruction). Pathogenicity and virulence were confirmed by inoculating four sweet corn hybrids in a greenhouse. The strains were Gram-negative rods, identified as Burkholderia gladioli based on biochemical profiles and molecular data (99% 16S rRNA+ recA similarity; phylogenetic clustering within the B. gladioli clade). In greenhouse trials, the strains induced brown spot lesions on the leaf sheaths of all tested hybrids, replicating field symptoms fulfilling Koch’s postulates. This is the first report of B. gladioli as the causal agent of brown spot on the leaf sheath of sweet corn in Mexico. The pathogen’s broad host range highlights its potential as an emerging threat to horticultural crops in the region. Full article

While silage maize (Zea mays L.) remains the dominant biogas feedstock crop in Germany, concerns about landscape homogenization and ecological risks have stimulated the search for more diverse energy crops. This study evaluated twelve amaranth genotypes (GT01–12; Amaranthus spp.) in southwest Germany using field experiments combined with biomass composition analysis and laboratory batch biogas assays. In contrast to earlier studies focusing primarily on the cultivar ‘Baernkraft’ (GT04), a broader set of genetic material was examined. Significant differences among GTs were observed for plant density, dry matter yield (DMY), dry matter content (DMC), and biomass composition. The most productive genotypes (GT09 and GT11) exceeded 10 Mg ha⁻¹ DMY, clearly outperforming Baernkraft. However, even these GTs did not reach the ≈28% DMC threshold considered necessary for reliable ensiling. Lignin concentrations ranged from 4.7% to 7.2% of dry matter. Methane concentrations remained relatively stable (54–55%), resulting in an average methane yield of 1788 ± 441 m³ CH₄ ha⁻¹ (maximum: 2677.8 m³ CH₄ ha⁻¹) across all genotypes and harvest dates. These findings indicate that amaranth may contribute to diversification of biogas cropping systems, although its agronomic and substrate-related performance remains inferior to that of maize under the conditions studied. Full article

Chitosan bioactivity has been widely evaluated in seed germination; however, its effects remain inconsistent. Some studies report biostimulant effects, while others manifest inhibitory effects, and some show no effect on germination. A major factor contributing to this poor reproducibility appears to be the variation in the degree of polymerization (X), the molar fraction of acetylation (f_A), and the chitosan concentration. However, it remains unclear whether controlling these parameters alone is sufficient to ensure consistent bioactivity in conventional polymeric chitosan samples. To elucidate this, maize seeds (Zea mays L. var. Jubilee) were soaked in chitosan solutions (pH 5) at concentrations ranging from 0.25 to 1.00% w/w for 4 h, as per the literature, to evaluate their effect on germination compared to a control (water). Nine chitosan samples were tested, differing in X (5558 to 17,161) and f_A (0.07 to 0.33). After germination, several response factors were measured, including fresh and dry biomass, root number, and root and epicotyl length. The results showed that chitosans with higher X values (9134 to 17,161) inhibit germination, decreasing it by up to a value of 3% ± 6 at 1.00% w/w. Chitosans with a lower X (5694 ± 131) exhibited a reduced inhibitory effect (63% ± 6 to 100%) across all evaluated concentrations. None of the treatments showed biostimulation; instead, chitosan consistently delayed the germination rate compared to the control, confirming an inhibitory effect regardless of the final percentage. Nevertheless, while X and concentration establish the general inhibitory trends, considerable residual variability suggests that these factors alone are insufficient to ensure consistent bioactivity. A Gauge Repeatability and Reproducibility (R&R) analysis provides preliminary evidence suggesting that molecular heterogeneity, specifically dispersity (Ð_X), is a key factor driving the observed inconsistencies and significantly affecting the reproducibility of the results within the scope of this study. Full article

Water scarcity is one of the main constraints on maize production in semiarid regions, making it essential to adopt management strategies that reconcile water savings, crop resilience, and economic viability. This study evaluated the effects of deficit irrigation strategies integrated with the use of bioinputs on physiological, productive, and economic parameters of maize grown under field conditions in the Brazilian semiarid region over two growing seasons (2023 and 2024). The experiment was conducted using a randomized complete block design with a split-plot arrangement. Irrigation strategies comprised full irrigation (FI; 100% of crop water requirements), continuous deficit irrigation (RD50%; 50% throughout the crop cycle), and stage-specific controlled deficit irrigation (50%) imposed during the vegetative (CDV50%), flowering/grain formation (CDF50%), and grain-filling (CDG50%) stages, while seed treatments involved inoculation with Bacillus aryabhattai, coinoculation with B. aryabhattai + Azospirillum brasilense, and control treatments. Physiological variables, yield components, water use efficiency, the crop sensitivity coefficient to water deficit (Ky), and economic indicators were assessed. Controlled deficits irrigation, particularly under CDV50%, maintained grain yield comparable to FI (6465.80 kg ha⁻¹, in second growing season), whereas RD50% reduced yield in 26%. Inoculation treatments enhanced gas exchange, carboxylation efficiency, and water use efficiency, resulting in higher agricultural income under specific production systems. The CDV50% strategy combined with coinoculation showed the greatest potential as a sustainable approach for maize production in semiarid environments and reduced the water use by up to 18.9%. Full article

The demand for sustainable weed management and the limited discovery of new herbicide molecules have led to high interest in plant-derived bioherbicides, such as the water residues (WRs) from the hydrodistillation of aromatic plants, which contain biologically active secondary metabolites. Here, the bioherbicidal activity of WRs of four aromatic plant species was investigated. Chemical composition of WRs was determined by solid-phase microextraction (SPME) coupled to gas chromatography–mass spectrometry (GC-MS), and their effect was assessed on seed germination and seedling growth characteristics of Avena sterilis, Echinochloa crus-galli, and Zea mays. Five concentrations, i.e., 0, 10, 20, 50, and 100% (v/v), with 100% representing pure WR, were tested. Phenolic monoterpenes dominate WRs in oregano and thyme, and oxygenated monoterpenes in laurel and lavender. Germination and growth responses were dose-dependent and species-specific. Oregano and lavender WRs exhibited the strongest inhibitory effect, reducing weed germination by 82% and 79%, respectively. In contrast, laurel extracts showed weaker germination inhibition. Across all tested species, germination delays were observed, making WRs a promising candidate for weed control. The results also showed that WR reduced root growth by up to 95% and shoot growth by 70–80%. Maize exhibited greater tolerance than the weed species, maintaining higher germination. Overall, WRs represent a promising tool for integrated weed management. Full article

With the rapid progression of global industrialization and urbanization, heavy metal contamination has emerged as a major global threat, especially cadmium pollution. Consequently, optimizing remediation measures has become a pivotal means to solve cadmium contamination. Compared to traditional physical and chemical remediation methods, microbial remediation has great potential in addressing cadmium pollution. In this study, a novel bacterial strain, Chryseobacterium sp. HGX-24, exhibiting high cadmium resistance was successfully isolated and screened from cadmium-contaminated environments. A preliminary discussion of the response mechanisms of this strain under cadmium stress is provided. Additionally, preliminarily explored the synergistic remediation of microbial-plant in cadmium-contaminated soil. Under conditions of high cadmium concentration, cadmium ions were effectively adsorbed by strain HGX-24 through extracellular polymers and functional groups on the cell wall surface, including −COOH, −CONH−, −NH, −OH, and >C=O. Extracellular proteins and polysaccharides were secreted by strain HGX-24 to regulate the adverse effects of heavy-metal cadmium ions on bacterial growth. Furthermore, the expression of genes such as antioxidant defense and ROS scavenging (katG, fabG, ybjT), Fe-S cluster assembly (sufB, sufD), sulfur metabolism (cysAU), amino acid metabolism (hisA, cysD, aspC), phenylacetic acid catabolism (paaC), and ribosomal proteins (rplC, rpsC, rpsL, rplA, rplY, rpmC) was regulated, affecting the synthesis and metabolism of membrane transporters (ABC transporters and efflux RND transporters), antioxidant enzymes (SOD, COT, POD), Fe-S clusters, thioredoxin family proteins, and ribosomal proteins, thereby enhancing resistance to cadmium toxicity. Moreover, strain HGX-24 was found to regulate the activities of redox enzymes in Zea mays L., thereby alleviating oxidative stress and reducing the negative feedback effects of reactive oxygen species in Z. mays. Full article

Negative plant–soil feedback (NPSF) drives yield decline in monocropping systems, yet how intraspecific competition modulates NPSF across planting densities remains unclear. We conducted a two-stage plant–soil feedback experiment using five crops (Triticum aestivum L., Zea mays L., Solanum lycopersicum L., Cucumis sativus L., and Panax notoginseng (Burkill) F.H. Chen) with contrasting NPSF intensities under four planting densities (30 × 30 to 8 × 8 cm). Crops with stronger NPSF (P. notoginseng) showed pronounced density-dependent biomass reductions, whereas those with moderate (S. lycopersicum, C. sativus) or low (Z. mays, T. aestivum) NPSF were largely density-insensitive. Given its sensitivity, P. notoginseng was used to explore mechanisms. High-density planting (8 × 8 cm) intensified NPSF, reducing seedling survival by 88.54% and biomass by 56.08% compared with low-density controls (30 × 30 cm). Microbiome profiling showed enrichment of pathogenic Fusarium spp. and depletion of beneficial Humicola spp. under high density. Metabolomic analysis identified linoleic acid and oleamide as key root exudates upregulated under high-density stress, which selectively stimulated Fusarium growth as preferred carbon sources. Collectively, these results reveal a density-dependent feedback in which intensified competition reshapes root exudation, promotes pathogen proliferation, and suppresses beneficial taxa, thereby amplifying NPSF. This provides mechanistic insights into microbially mediated NPSF under density stress and highlights the importance of optimizing planting density to sustain crop productivity. Full article

The field experiment was conducted in 2016–2018 at the Department of Agronomy of the Poznań University of Life Sciences on the fields of the Research and Education Centre in Gorzyń, Złotniki branch. It was a single-factor experiment with six sowing dates of an ultra-early maize variety: A1—12 April, A2—26 April, A3—10 May, A4—24 May, A5—7 June, and A6—21 June. Seeds of the maize variety ‘Pyroxenia’ were used in the experiment. This variety is characterized by extremely early maturity (FAO 130), rapid initial development and elongation growth. Delaying the maize sowing date from A1 to A2 resulted in a 16.5% reduction in starch content in the silage dry matter, and a 14.6% increase in the ADF (Acid Detergent Fiber) fiber fraction. The difference in milk production per hectare between maize sown on date A1 and date A6 was 14,189.51 kg/ha, representing 97.1%. Delaying the maize sowing date led to an increase in the abundance of Clostridium spp. in silages, which are responsible for increased losses of dry matter, including starch. No butyric acid was detected in the silages as a final product of butyric fermentation. The low abundance of bacteria from the family Enterobacteriaceae in the silages indicated that they were well prepared. Silages prepared from maize sown at later dates were characterized by a higher abundance of undesirable mold fungi, which are responsible for dry matter losses, including starch. The coefficient of determination showed that 38.54% of the variation in silage starch content was explained by variation in mold abundance in the silage. According to the Flieg–Zimmer scale, all silages received a very good rating, regardless of maize sowing date. Full article

Optimizing tillage and fertilization practices is of vital importance for enhancing soil carbon retention, improving soil quality and increasing crop productivity in the intensive wheat (Triticum aestivum L.)–maize (Zea mays L.) double cropping system (WM). However, the combined effects of subsoiling (ST) and liquid manure (LM) application on yield sustainability and the dynamic changes in labile organic carbon (LOC) fractions (LOCs) remain insufficiently quantified in WM in the North China Plain (NCP). A two-year field experiment evaluated the responses of grain yields, the sustainable yield index (SYI), soil organic carbon (SOC), LOCs, C pool management indexes (CPMIs), and the soil quality index (SQI) to both patterns of tillage [conventional shallow rotary tillage (RT) and ST] and fertilization [conventional fertilization (CF), LM broadcast (LMB), and LM injection (LMI)] in WM in the NCP. Compared with RT, ST significantly enhanced crop grain yields (3.5~4.1%) and the annual SYI (4.1%) (p < 0.05). The contents of SOC, total labile OC (TLOC), high LOC (HLOC), and medium LOC (MLOC) and the values of SQI were higher in soil layers at both 0–20 cm and 20–40 cm under ST than those under RT. Compared with CF, LMI significantly enhanced grain yields (5.8~6.1%) and the annual SYI (5.4%). LMI significantly increased the contents of SOC, TLOC, HLOC, and MLOC and the SQI values in both soil layers relative to CF, while no significant difference was observed for grain yields, the annual SYI, and the SQI between LMB and CF. The higher contents of SOC and LOC led to an increase in the values of CPMIs based on TLOC (TCPMI), HLOC (HCPMI), and MLOC (MCPMI). The combination of both ST and LMI enhanced SOC retention through the increase in recalcitrant organic carbon (ROC) content and the transformation process of LOCs. It was obvious that HLOC and MLOC affected SOC, HCPMI, and MCPMI in the soil layers at both 0–20 cm and 20–40 cm, and thus can be regarded as sensitive indicators reflecting the dynamic changes in SOC and soil quality. Therefore, the combination of subsoiling and liquid manure injection can promote labile OC transformation, SOC retention, soil quality, and yield sustainability, providing an effective management strategy for the achievement of sustained agricultural production in the NCP or other regions with similar conditions. Full article

Cadmium is an element that is unnecessary for the functioning of plant and animal organisms, and its widespread presence in the environment poses a serious threat to human and animal health. Therefore, effective methods are being sought to remediate soils contaminated with this element, including through the enrichment of degraded soils with organic matter. To this end, the effectiveness of selected organic sorbents, including starch, fermented bark, compost and humic acids, in mitigating the transfer of cadmium and other heavy metals from soil to plants was assessed. Model studies compared the effects of 15 and 30 mg of cadmium (Cd) per kg of soil with an uncontaminated control sample. The sorbents were applied on a carbon basis at a rate of 3 g C per kg of soil. The test plant was Zea mays. Cadmium was found to significantly impair plant growth, causing reductions of 21%, 85%, and 77% in leaf greenness, aboveground biomass and root biomass, respectively. Excess cadmium increased the translocation of lead, chromium, copper, nickel, zinc, iron, and manganese from the roots to the aboveground parts of the plant, while simultaneously limiting their uptake. All of the organic sorbents tested reduced the negative impact of cadmium on leaf greenness, except starch. Compost and HumiAgra significantly improved the condition of Zea mays plants weakened by cadmium exposure. Cadmium contamination increased soil acidification. pH was positively correlated with maize yield and the SPAD leaf greenness index and negatively correlated with the cadmium translocation index and cadmium content in the aboveground parts of maize. Compost and humic acids are among the most effective and practically feasible approaches for reducing cadmium bioavailability in soil and its accumulation in Zea mays, and are therefore recommended for the remediation of cadmium-contaminated soils. Full article