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19 pages, 312 KB  
Review
On Behalf of the Wolf: Niche Construction and Indigenous Concepts of Creation
by Raymond Pierotti
Humans 2026, 6(1), 7; https://doi.org/10.3390/humans6010007 - 25 Feb 2026
Viewed by 256
Abstract
There have been numerous attempts to examine Indigenous cultures from a scientific and evolutionary perspective. In this work, however, there has been little acknowledgment of how the study of biological evolution is changing. I examine evidence of the way Indigenous cultures think about [...] Read more.
There have been numerous attempts to examine Indigenous cultures from a scientific and evolutionary perspective. In this work, however, there has been little acknowledgment of how the study of biological evolution is changing. I examine evidence of the way Indigenous cultures think about nonhumans and examine concepts of creation and creator figures in relation to Niche Construction, a 21st century evolutionary concept that examines how organisms shape both their own environments and those of other species by studying how Natural Selection can act upon how most organisms impact the survival and existence of other species. I focus this comparison on how many Indigenous Plains cultures of North America regard wolves as being creator figures within the context of the way they experience their environments. Ecological studies revealed that in 30 years since wolf reintroduction to Yellowstone, this species has reshaped the ecology of many other species in the park ecosystem. I argue that in the belief systems of Indigenous peoples, this restructuring is tantamount to an Act of Creation, and that Indigenous Americans recognized that wolves filled both this role, as well as a role in helping Indigenous cultures adjust to the environments of North America as they arrived on this continent over the last 20,000 years. I also consider the relationship from the wolves’ perspective. This concept of creation is rooted in ecology and evolutionary biology, and does not involve supernatural anthropomorphic beings the way Western stories of creation do. Full article
14 pages, 587 KB  
Article
Bioacoustic Detection of Wolves Using AI (BirdNET, Cry-Wolf and BioLingual)
by Johanne Holm Jacobsen, Pietro Orlando, Line Østergaard Jensen, Sussie Pagh and Cino Pertoldi
Animals 2026, 16(2), 175; https://doi.org/10.3390/ani16020175 - 7 Jan 2026
Viewed by 756
Abstract
Rising numbers of wolf (Canis lupus) populations make traditional, resource-intensive methods of wolf monitoring increasingly challenging and often insufficient. This study explores how wolf howls can be used as a new monitoring tool for wolves by applying Artificial Intelligence (AI) methods [...] Read more.
Rising numbers of wolf (Canis lupus) populations make traditional, resource-intensive methods of wolf monitoring increasingly challenging and often insufficient. This study explores how wolf howls can be used as a new monitoring tool for wolves by applying Artificial Intelligence (AI) methods to detect and classify wolf howls from acoustic recordings, thereby improving the effectiveness of wolf population monitoring. Three AI approaches are evaluated: BirdNET, Yellowstone’s Cry-Wolf project system, and BioLingual. Data were collected using Song Meter SM4 (SM4) audio recorders in a known wolf territory in Klelund Dyrehave, Denmark, and manually validated to establish a ground truth of 260 wolf howls. Results demonstrate that while AI solutions currently do not achieve the complete precision or overall accuracy of expert manual analysis, they offer tremendous efficiency gains, significantly reducing processing time. BirdNET achieved the highest recall at 78.5% (204/260 howls detected), though with a low precision of 0.007 (resulting in 28,773 false positives). BioLingual detected 61.5% of howls (160/260) with 0.005 precision (30,163 false positives), and Cry-Wolf detected 59.6% of howls (155/260) with 0.005 precision (30,099 false positives). Crucially, a combined approach utilizing all three models achieved a 96.2% recall (250/260 howls detected). This suggests that while AI solutions primarily function as powerful human-aided data reduction tools rather than fully autonomous detectors, they represent a valuable, scalable, and non-invasive complement to traditional methods in wolf research and conservation, making large-scale monitoring more feasible. Full article
(This article belongs to the Section Animal System and Management)
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15 pages, 389 KB  
Article
Increasing Positive Perception of Disability Through Depictions of Animals with Disabilities
by Cameron T. Whitley, Marta Burnet, Em Sherwood, Denny Dulaney, Alexander Jones, Courtney Cordova, Emma Hindes, Katya Ankoudinova, Brooklyn Wehr, Corin Yates, Brooke Tucker, Tut Fuentevilla, Caitlin Allessi, Tess Busch, Kevin Kollar, Michelle Hanenburg and Natalie Stier
Animals 2025, 15(13), 1861; https://doi.org/10.3390/ani15131861 - 24 Jun 2025
Cited by 1 | Viewed by 1824
Abstract
Across the globe, many animals with disabilities live in zoos and aquariums, yet these institutions often face difficult questions about how to share those stories with the public in a way that raises awareness and minimizes concern about the animals’ well-being. This study [...] Read more.
Across the globe, many animals with disabilities live in zoos and aquariums, yet these institutions often face difficult questions about how to share those stories with the public in a way that raises awareness and minimizes concern about the animals’ well-being. This study explored whether anthropomorphic narrative signage could help visitors form meaningful emotional connections with these animals and, in doing so, encourage greater understanding and support for people with disabilities. Drawing on ideas from contact and transfer theories, we conducted observational research in zoo settings, comparing visitor reactions to no signage, detailed signage, and simple signage. We found that simple signage resulted in fewer negative comments, fewer questions about the animal’s health, and less frustration directed at the organization. In a related survey experiment, participants who saw a photo and brief story about an animal with a disability were more likely to express empathy and hold more positive views toward both animals and people with disabilities. These findings highlight the power of accessible messaging in informal learning spaces to reduce stigma and promote inclusion. The project was led by a diverse, interdisciplinary team of zoo professionals and disability scholars, most of whom bring personal experience with physical or cognitive disabilities to the work. Full article
(This article belongs to the Section Zoo Animals)
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26 pages, 41765 KB  
Article
Trophic State Evolution of 45 Yellowstone Lakes over Two Decades: Field Data and a Longitudinal Study
by A. Woodruff Miller, Gustavious P. Williams, Rachel Huber Magoffin, Xueyi Li, Taylor Miskin, Amin Aghababaei, Pitamber Wagle, Abin Raj Chapagain, Yubin Baaniya, Peter D. Oldham, Samuel J. Oldham, Tyler Peterson, Lyle Prince, Kaylee B. Tanner, Anna C. Cardall and Daniel P. Ames
Water 2025, 17(11), 1627; https://doi.org/10.3390/w17111627 - 27 May 2025
Cited by 1 | Viewed by 1466
Abstract
From 1998 to 2024, we collected field samples at 45 selected lakes in Yellowstone National Park during the months of April through October. We estimated inflows, outflows, and Secchi depths for most lakes. We analyzed the samples for total phosphorous and chlorophyll-a. We [...] Read more.
From 1998 to 2024, we collected field samples at 45 selected lakes in Yellowstone National Park during the months of April through October. We estimated inflows, outflows, and Secchi depths for most lakes. We analyzed the samples for total phosphorous and chlorophyll-a. We used these data to classify the lake trophic states using the Carlson TSI (CTSI), Vollenweider (VW), and Larsen–Mercier (LM) models to assess how trophic states evolved over this 26-year period. This longitudinal dataset is unique because of its extensive 26-year time span gathered from difficult-to-access locations. We found that the data depended on lake size, lake elevation, and the month when data were collected. Most of the lakes exhibit mesotrophic conditions, with variations depending on the trophic state model used. The CTSI distribution shows median values typically between 40 and 55, while the VW and LM index distributions present a somewhat similar pattern but with fewer lakes categorized due to data requirements. We visualized temporal patterns using heatmaps and analyzed trends using the Mann–Kendall test to identify trends and if they were statistically significant. We found only four lakes with statistically significantly increasing trends and two with decreasing trends. Because of the difference in the months when data were collected, the increasing trends in three of the lakes are less certain. We found that, except for four lakes, the trophic states of Yellowstone lakes were maintained or improved over this ~20-year period. Only the trophic state of Nymph Lake clearly deteriorated. The remaining lakes had stable trophic states, with three having weak evidence of worsening conditions. This long-term dataset, which we publish for others’ use, provides an opportunity to better understand eutrophication processes and water quality dynamics in Yellowstone, providing critical information for park management and conservation efforts. Full article
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33 pages, 1969 KB  
Article
Collaborative Adaptive Management in the Greater Yellowstone Ecosystem: A Rangeland Living Laboratory at the US Sheep Experiment Station
by Hailey Wilmer, Jonathan Spiess, Patrick E. Clark, Michelle Anderson, Amira Burns, Arica Crootof, Lily Fanok, Tracy Hruska, Bruce J. Mincher, Ryan S. Miller, William Munger, Christian J. Posbergh, Carrie S. Wilson, Eric Winford, Jessica Windh, Nicole Strong, Marlen Eve and J. Bret Taylor
Sustainability 2025, 17(7), 3086; https://doi.org/10.3390/su17073086 - 31 Mar 2025
Cited by 3 | Viewed by 2595
Abstract
Social conflict over rangeland-use priorities, especially near protected areas, has long pitted environmental and biodiversity conservation interests against livestock livelihoods. Social–ecological conflict limits management adaptation and creativity while reinforcing social and disciplinary divisions. It can also reduce rancher access to land and negatively [...] Read more.
Social conflict over rangeland-use priorities, especially near protected areas, has long pitted environmental and biodiversity conservation interests against livestock livelihoods. Social–ecological conflict limits management adaptation and creativity while reinforcing social and disciplinary divisions. It can also reduce rancher access to land and negatively affect wildlife conservation. Communities increasingly expect research organizations to address complex social dynamics to improve opportunities for multiple ecosystem service delivery on rangelands. In the Greater Yellowstone Ecosystem (GYE), an area of the western US, long-standing disagreements among actors who argue for the use of the land for livestock and those who prioritize wildlife are limiting conservation and ranching livelihoods. Researchers at the USDA-ARS US Sheep Experiment Station (USSES) along with University and societal partners are responding to these challenges using a collaborative adaptive management (CAM) methodology. The USSES Rangeland Collaboratory is a living laboratory project leveraging the resources of a federal range sheep research ranch operating across sagebrush steppe ecosystems in Clark County, Idaho, and montane/subalpine landscapes in Beaverhead County, Montana. The project places stakeholders, including ranchers, conservation groups, and government land managers, in the decision-making seat for a participatory case study. This involves adaptive management planning related to grazing and livestock–wildlife management decisions for two ranch-scale rangeland management scenarios, one modeled after a traditional range sheep operation and the second, a more intensified operation with no use of summer ranges. We discuss the extent to which the CAM approach creates opportunities for multi-directional learning among participants and evaluate trade-offs among preferred management systems through participatory ranch-scale grazing research. In a complex system where the needs and goals of various actors are misaligned across spatiotemporal, disciplinary, and social–ecological scales, CAM creates a structure and methods to focus on social learning and land management knowledge creation. Full article
(This article belongs to the Section Sustainable Management)
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23 pages, 13840 KB  
Article
A Convection-Permitting Regional Climate Simulation of Changes in Precipitation and Snowpack in a Warmer Climate over the Interior Western United States
by Yonggang Wang, Bart Geerts, Changhai Liu and Xiaoqin Jing
Climate 2025, 13(3), 46; https://doi.org/10.3390/cli13030046 - 24 Feb 2025
Cited by 2 | Viewed by 1870
Abstract
This study investigates the impacts of climate change on precipitation and snowpack in the interior western United States (IWUS) using two sets of convection-permitting Weather Research and Forecasting model simulations. One simulation represents the ~1990 climate, and another represents an ~2050 climate using [...] Read more.
This study investigates the impacts of climate change on precipitation and snowpack in the interior western United States (IWUS) using two sets of convection-permitting Weather Research and Forecasting model simulations. One simulation represents the ~1990 climate, and another represents an ~2050 climate using a pseudo-global warming approach. Climate perturbations for the future climate are given by the CMIP5 ensemble-mean global climate models under the high-end emission scenario. The study analyzes the projected changes in spatial patterns of seasonal precipitation and snowpack, with particular emphasis on the effects of elevation on orographic precipitation and snowpack changes in four key mountain ranges: the Montana Rockies, Greater Yellowstone area, Wasatch Range, and Colorado Rockies. The IWUS simulations reveal an increase in annual precipitation across the majority of the IWUS in this warmer climate, driven by more frequent heavy to extreme precipitation events. Winter precipitation is projected to increase across the domain, while summer precipitation is expected to decrease, particularly in the High Plains. Snow-to-precipitation ratios and snow water equivalent are expected to decrease, especially at lower elevations, while snowpack melt is projected to occur earlier by up to 26 days in the ~2050 climate, highlighting significant impacts on regional water resources and hydrological management. Full article
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18 pages, 4851 KB  
Article
The Implications of Fremont Pottery in Montana
by Lawrence Loendorf and Kathleen Rodrigues
Arts 2025, 14(1), 17; https://doi.org/10.3390/arts14010017 - 12 Feb 2025
Viewed by 2516
Abstract
Fremont pottery was recovered at the Valley of the Shields rock art site in Montana and dated by luminescence at circa A.D. 1500. The Fremont archaeological culture of northern Colorado and Utah is thought to have dispersed circa A.D. 1350, meaning that Montana [...] Read more.
Fremont pottery was recovered at the Valley of the Shields rock art site in Montana and dated by luminescence at circa A.D. 1500. The Fremont archaeological culture of northern Colorado and Utah is thought to have dispersed circa A.D. 1350, meaning that Montana Fremont pottery appeared late in time. Recent research suggests that the Fremont spoke a Tanoan language, and when they disbanded, major groups moved east to join the Rio Grande Pueblos, while another group, the Kiowa, who spoke a Tanoan language are believed to have moved north to the region around Yellowstone National Park. Importantly, artifacts found with Montana Fremont pottery are made of the same material type that was found in an excavation beneath a panel of Castle Gardens-style rock art figures at Valley of the Shields. These combined discoveries suggest that Montana Fremont pottery is the product of the proto-Kiowa, and they are the most likely group to have made Castle Gardens-style rock art shields. Full article
(This article belongs to the Special Issue Advances in Rock Art Studies)
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15 pages, 4096 KB  
Article
Yellowstone Wildfires Increased Stream Ion Concentrations and Export
by Isabella G. Sadler, Lusha M. Tronstad, Christine Fisher, Robert O. Hall and Todd M. Koel
Nitrogen 2024, 5(4), 1181-1195; https://doi.org/10.3390/nitrogen5040075 - 20 Dec 2024
Cited by 3 | Viewed by 1851
Abstract
Wildfires in the western U.S. have increased in severity and duration in recent decades. Severe wildfires can enhance the rates of nutrient mineralization, causing large exports of inorganic nitrogen and other ions from forests to streams. Measuring the degree to which streams respond [...] Read more.
Wildfires in the western U.S. have increased in severity and duration in recent decades. Severe wildfires can enhance the rates of nutrient mineralization, causing large exports of inorganic nitrogen and other ions from forests to streams. Measuring the degree to which streams respond to severe, stand-replacing wildfires is critical to estimate in ecosystems prone to disturbance. In 2003, two severe crown wildfires burned in Yellowstone National Park, WY, USA. We studied the extent to which these fires increased nitrogen (ammonium, nitrate and nitrite), sulfate, chloride, and total dissolved phosphorus concentrations and export in three watersheds prior to and during the first four years post-fire. We measured higher concentrations of most ions after wildfire, and nitrate and chloride concentrations increased the most, increasing > 1000 µg/L. Concentrations of nitrate (≤146 times pre-fire concentrations), total dissolved nitrogen (≤11 times), chloride (≤9 times), and total dissolved phosphorus (≤7 times) were higher four years post-fire than before the wildfires burned. Exports of nitrate (≤1392 times), sulfate (≤14 times), and chloride (≤37 times) were also higher after wildfire, while nitrite (≤2.9 times) and ammonium (≤6.4 times) increased to a lesser degree. Stream concentrations of most ions were higher in watersheds that had a larger percent of the area burned. Comparing ion concentrations in streams before and after severe wildfires provides critical information to managers as the climate warms and the frequency of fire-conductive weather increases. Full article
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16 pages, 4967 KB  
Article
Effects of Solid Dairy Manure Application on Greenhouse Gas Emissions and Corn Yield in the Upper Midwest, USA
by Eric Young and Jessica Sherman
Sustainability 2024, 16(24), 11171; https://doi.org/10.3390/su162411171 - 20 Dec 2024
Viewed by 1620
Abstract
Dairy manure is an important nitrogen (N) source for crops, but its role in greenhouse gas (GHG) emissions and farm sustainability is not fully understood. We evaluated the effects of application of two dairy manure sources (bedded pack heifer, BP, and separated dairy [...] Read more.
Dairy manure is an important nitrogen (N) source for crops, but its role in greenhouse gas (GHG) emissions and farm sustainability is not fully understood. We evaluated the effects of application of two dairy manure sources (bedded pack heifer, BP, and separated dairy solids, SDS) on corn silage yield and GHG emissions (carbon dioxide, CO2; methane, CH4; nitrous oxide, N2O) compared to a urea-fertilizer-only control (80 kg N ha−1 yr−1). The BP and SDS were applied at 18.4 and 19.4 Mg dry matter ha−1 in fall 2020 in the final year of ryegrass production. No-till corn was planted from 2021 to 2023, and GHG emissions were measured each season (from May to November). The results showed significantly greater CO2-C emissions for BP in 2021 and no differences in 2022 or 2023. A small N2O-N emission increase for BP occurred in the spring after application; however, seasonal fluxes were low or negative. Mean CH4-C emissions ranged from 2 to 7 kg ha−1 yr−1 with no treatment differences. Lack of soil aeration appeared to be an important factor affecting seasonal N2O-N and CH4-C emissions. The results suggest that GHG models should account for field-level nutrient management factors in addition to soil aeration status. Full article
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20 pages, 2241 KB  
Article
Can the Artificial Release of Fluorinated Gases Offset Global Cooling Due to Supervolcanic Eruptions?
by Yangyang Xu, Nathanael P. Ribar, Jeffrey Sachnik, Gunnar W. Schade, Andrew John Lockley, Yi Ge Zhang, Pengfei Yu, Jianxin Hu and Guus J. M. Velders
Atmosphere 2024, 15(11), 1322; https://doi.org/10.3390/atmos15111322 - 2 Nov 2024
Cited by 2 | Viewed by 3149
Abstract
Large volcanic eruptions, such as the prehistoric Yellowstone eruption, induce abrupt global cooling—by some estimates at a rate of ~1 °C/year, lasting for more than a decade. An abrupt global cooling of several °C—even if only lasting a few years—would present immediate, drastic [...] Read more.
Large volcanic eruptions, such as the prehistoric Yellowstone eruption, induce abrupt global cooling—by some estimates at a rate of ~1 °C/year, lasting for more than a decade. An abrupt global cooling of several °C—even if only lasting a few years—would present immediate, drastic stress on biodiversity and food production. This cooling poses a global catastrophic risk to human society beyond the immediate and direct impact of eruptions. Using a simple climate model, this paper discusses the possibility of counteracting large volcanic cooling with the intentional release of greenhouse gases. Longer-lived compounds (e.g., CO2 and CH4) are unsuitable for this purpose, but selected fluorinated gases (F-gases), either individually or in combinations, could be released at gigaton scale to offset large volcanic cooling substantially. We identify candidate F-gases (e.g., C4F6 and CH3F) and derive radiative and chemical properties of ‘ideal’ compounds matching specific cooling events. Geophysical constraints on manufacturing and stockpiling due to mineral availability are considered, alongside technical and economic implications based on present-day market assumptions. The effects and uncertainty due to atmospheric chemistry related to aerosol injection, F-gases release, and solar dimming are discussed in the context of large volcanic perturbation. The caveats and future steps using more complex chemistry–climate models are discussed. Despite the speculative nature of the magnitude and composition of F-gases, our conceptual analysis has implications for testing the possibility of mitigating certain global catastrophic cooling risks (e.g., nuclear winter, asteroid impact, and glacier transition) via intentional intervention. Full article
(This article belongs to the Section Aerosols)
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10 pages, 903 KB  
Article
A Test of Activated Carbon and Soil Seed Enhancements for Improved Sub-Shrub and Grass Seedling Survival With and Without Herbicide Application
by Lauren N. Svejcar, Trace E. Martyn, Hayley R. Edlund and Kirk W. Davies
Plants 2024, 13(21), 3074; https://doi.org/10.3390/plants13213074 - 1 Nov 2024
Cited by 3 | Viewed by 2204
Abstract
Re-establishing native plants while controlling invasive species is a challenge for many dryland restoration efforts globally. Invasive plants often create highly competitive environments so controlling them is necessary for effective establishment of native species. In the sagebrush steppe of the United States, invasive [...] Read more.
Re-establishing native plants while controlling invasive species is a challenge for many dryland restoration efforts globally. Invasive plants often create highly competitive environments so controlling them is necessary for effective establishment of native species. In the sagebrush steppe of the United States, invasive annual grasses are commonly controlled with herbicide treatments. However, the same herbicides that control invasive annual grasses also impact the native species being planted. As such, carbon-based seed technologies to protect native seeds from herbicide applications are being trialed. In addition to controlling invasive species, ensuring good seed-to-soil contact is important for effective establishment of native species. In this grow room study, we explored the impact of different seed ameliorations when no herbicide was applied and when herbicide was applied. We selected two native species that are important to the sagebrush steppe for this study—the sub-shrub Krascheninnikovia lanata and the perennial bunchgrass Pseudoroegneria spicata—and used three different seed ameliorations—seed pelleting with local soil alone, local soil plus activated carbon and activated carbon alone—to ensure both greater seed-to-soil contact and protection against herbicides. Shoot and root biomass data were collected eight weeks after planting. We found that when herbicide was not applied, K. lanata had the strongest response to the soil alone amelioration, while P. spicata had the strongest response to the activated carbon alone amelioration. However, when herbicide was applied, K. lanata performed best with the soil plus activated carbon treatments, with an average 1500% increase in biomass, while P. spicata performed best with the activated carbon alone treatments, with an over 4000% increase in biomass, relative to bare seed. The results from our study indicate that there is a positive effect of local soils and activated carbon as seed ameliorations, and further testing in the field is needed to understand how these ameliorations might perform in actual restoration scenarios. Full article
(This article belongs to the Special Issue Innovative Seed Enhancement Technologies)
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11 pages, 1033 KB  
Article
U-Pb LA-ICP-MS Zircon Dating of Crustal Xenoliths: Evidence of the Archean Lithosphere Beneath the Snake River Plain
by William P. Leeman, Jeffrey D. Vervoort and S. Andrew DuFrane
Minerals 2024, 14(6), 578; https://doi.org/10.3390/min14060578 - 30 May 2024
Cited by 2 | Viewed by 1879 | Correction
Abstract
New U-Pb zircon ages are reported for granulite facies crustal xenoliths brought to the surface by mafic lavas in the Snake River Plain. All samples yield Meso-to-Neoarchean ages (2.4–3.6 Ga) that significantly expand the known extent of the Archean Wyoming Craton at least [...] Read more.
New U-Pb zircon ages are reported for granulite facies crustal xenoliths brought to the surface by mafic lavas in the Snake River Plain. All samples yield Meso-to-Neoarchean ages (2.4–3.6 Ga) that significantly expand the known extent of the Archean Wyoming Craton at least as far west as the west-central Snake River Plain. Most zircon populations indicate multiple growth episodes with complexity increasing eastward, but they bear no record of major Phanerozoic magmatic episodes in the region. To extrapolate this work further west to the inferred craton boundary, zircons from southwestern Idaho batholith granodiorites were also analyzed. Although most batholith zircons record Cretaceous formation ages, all samples have zircons with inherited cores—with some recording Proterozoic ages (approaching 2 Ga). These data enhance our perspectives regarding lithosphere architecture beneath southern Idaho and adjacent areas and its possible influence on Cenozoic magmatism associated with the Snake River Plain–Yellowstone “melting anomaly”. Full article
(This article belongs to the Section Mineral Geochemistry and Geochronology)
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27 pages, 7292 KB  
Article
Mantle Sources and Geochemical Evolution of the Picture Gorge Basalt, Columbia River Basalt Group
by Emily B. Cahoon, Martin J. Streck, Richard W. Carlson and Ilya N. Bindeman
Minerals 2024, 14(5), 440; https://doi.org/10.3390/min14050440 - 23 Apr 2024
Cited by 3 | Viewed by 2526
Abstract
The Columbia River Basalt Group (CRBG) is the youngest continental flood basalt province, proposed to be sourced from the deep-seated plume that currently resides underneath Yellowstone National Park. If so, the earliest erupted basalts from this province, such as those in the Picture [...] Read more.
The Columbia River Basalt Group (CRBG) is the youngest continental flood basalt province, proposed to be sourced from the deep-seated plume that currently resides underneath Yellowstone National Park. If so, the earliest erupted basalts from this province, such as those in the Picture Gorge Basalt (PGB), aid in understanding and modeling plume impingement and the subsequent evolution of basaltic volcanism. Using geochemical and isotopic data, this study explores potential mantle sources and magma evolution of the PGB. Long known geochemical signatures of the PGB include overall large ion lithophile element (LILE) enrichment and relative depletion of high field strength elements (HFSE) typical of other CRBG main-phase units. Basaltic samples of the PGB have 87Sr/86Sr ratios on the low end of the range displayed by other CRBG lavas and mantle-like δ18O values. The relatively strong enrichment of LILE and depletion of HFSE coupled with depleted isotopic signatures suggest a metasomatized upper mantle as the most likely magmatic source for the PGB. Previous geochemical modeling of the PGB utilized the composition of two high-MgO primitive dikes exposed in the northern portion of the Monument Dike swarm as parental melt. However, fractionation of these dike compositions cannot generate the compositional variability illustrated by basaltic lavas and dikes of the PGB. This study identifies a second potential parental PGB composition best represented by basaltic flows in the extended spatial distribution of the PGB. This composition also better reflects the lowest stratigraphic flows identified in the previously mapped extent of the PGB. Age data reveal that PGB lavas erupted first and throughout eruptions of main-phase CRBG units (Steens, Imnaha, Grande Ronde Basalt). Combining geochemical signals with these age data indicates cyclical patterns in the amounts of contributing mantle components. Eruption of PGB material occurred in two pulses, demonstrated by a ~0.4 Ma temporal gap in reported ages, 16.62 to 16.23 Ma. Coupling ages with observed geochemical signals, including relative elemental abundances of LILE, indicates increased influence of a more primitive, potentially plume-like source with time. Full article
(This article belongs to the Special Issue Large Igneous Provinces: Research Frontiers)
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14 pages, 1463 KB  
Article
Characterization of a Novel Hyperthermophilic GH1 β-Glucosidase from Acidilobus sp. and Its Application in the Hydrolysis of Soybean Isoflavone Glycosides
by Jinjian He, Yuying Li, Xihang Sun, Dinghui Zuo, Mansheng Wang, Xia Zheng, Pinglian Yu and Pengjun Shi
Microorganisms 2024, 12(3), 533; https://doi.org/10.3390/microorganisms12030533 - 7 Mar 2024
Cited by 4 | Viewed by 2420
Abstract
A putative β-glucosidase gene, BglAc, was amplified from Acidilobus sp. through metagenome database sampling from a hot spring in Yellowstone National Park. BglAc is composed of 485 amino acid residues and bioinformatics analysis showed that it belongs to the GH1 family of [...] Read more.
A putative β-glucosidase gene, BglAc, was amplified from Acidilobus sp. through metagenome database sampling from a hot spring in Yellowstone National Park. BglAc is composed of 485 amino acid residues and bioinformatics analysis showed that it belongs to the GH1 family of β-glucosidases. The gene was successfully expressed in Escherichia coli with a molecular weight of approximately 55.3 kDa. The purified recombinant enzyme showed the maximum activity using p-nitrophenyl-β-D-glucopyranoside (pNPG) as the substrate at optimal pH 5.0 and 100 °C. BglAc exhibited extraordinary thermostability, and its half-life at 90 °C was 6 h. The specific activity, Km, Vmax, and Kcat/Km of BglAc toward pNPG were 357.62 U mg−1, 3.41 mM, 474.0 μmol min−1·mg−1, and 122.7 s−1mM−1. BglAc exhibited the characteristic of glucose tolerance, and the inhibition constant Ki was 180.0 mM. Furthermore, a significant ethanol tolerance was observed, retaining 96% relative activity at 10% ethanol, and even 78% at 20% ethanol, suggesting BglAc as a promising enzyme for cellulose saccharification. BglAc also had a strong ability to convert the major soybean isoflavone glycosides (daidzin, genistin, and glycitin) into their corresponding aglycones. Overall, BglAc was actually a new β-glucosidase with excellent thermostability, ethanol tolerance, and glycoside hydrolysis ability, indicating its wide prospects for applications in the food industry, animal feed, and lignocellulosic biomass degradation. Full article
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13 pages, 2752 KB  
Article
Habitat Characteristics, Distribution, and Abundance of Cicindelidia haemorrhagica (LeConte) (Coleoptera: Cicindelidae) in Yellowstone National Park
by Kelly A. Willemssens, John L. Bowley, Laissa Cavallini, Erik Oberg, Robert K. D. Peterson and Leon G. Higley
Insects 2024, 15(1), 15; https://doi.org/10.3390/insects15010015 - 29 Dec 2023
Cited by 2 | Viewed by 1964
Abstract
We observed the tiger beetle species, Cicindelidia haemorrhagica (LeConte), foraging in and reproducing near the thermal pools of Yellowstone National Park (YNP). Although this species was recorded in YNP more than 130 years ago, its distribution, ecology, and association with thermal features are [...] Read more.
We observed the tiger beetle species, Cicindelidia haemorrhagica (LeConte), foraging in and reproducing near the thermal pools of Yellowstone National Park (YNP). Although this species was recorded in YNP more than 130 years ago, its distribution, ecology, and association with thermal features are unknown. Therefore, we examined the distribution and habitat characteristics of C. haemorrhagica and evaluated methods for studying its abundance. Given the extreme environments in which these beetles live, typical methods to estimate abundance are challenging. We used a series of presence/absence studies and observations to assess distribution and recorded temperature and pH measurements to determine habitat characteristics. We also conducted visual counts, light trapping, and mark/recapture experiments to assess abundance. The inability to capture C. haemorrhagica with lights led to a phototaxis experiment, which showed minimal attraction to light. Cicindelidia haemorrhagica was found throughout YNP, but it was exclusively associated with thermal springs. The thermal springs ranged from pH 2.7 to 9.0 with temperatures from 29.1 to 75.0 °C and had varying metal concentrations in soil and water. However, all thermal springs with C. haemorrhagica had barren soil with a gradual slope toward the thermal water. Specifically, habitats were thermal pools with gradual margins (a less than five-degree slope) and thermal (i.e., heated) soils for larval burrows by thermal springs or pools. Population sizes of C. haemorrhagica ranged between 500 and 1500 individuals based on visual counts. Full article
(This article belongs to the Section Insect Ecology, Diversity and Conservation)
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