Search Results (499)

Background/Objectives: Antimicrobial resistance (AMR) in wastewater represents a valuable reservoir of information for wastewater-based epidemiology (WBE) and a major environmental and public health concern, as wastewater treatment plants (WWTPs) are recognized hotspots for the accumulation and dissemination of antimicrobial resistance genes (ARGs). Within the One Health framework, and to better understand the contribution to AMR spread and the potential of metagenomic surveillance, this study aimed to characterize the taxonomic, functional, and resistome profiles of three WWTPs in Sicily, specifically those located in Catania, Giarre, and Syracuse. Methods: Sixty-nine composite influent samples were collected between February 2022 and December 2023. Shotgun metagenomic sequencing was performed on the Illumina NovaSeq platform. Bioinformatic analyses were conducted to assess microbial community composition, functional pathways, and ARG prevalence across sites. Results: Dominant genera included Aliarcobacter, Bacteroides, and Acinetobacter. Site-specific taxonomic variations reflected differences in local microbial ecology. Functional profiling revealed enrichment in membrane-associated, ribosomal, and energy metabolism pathways, consistent with the expected functional redundancy of wastewater microbiomes. Resistome analysis detected a diverse and ubiquitous array of ARGs, dominated by β-lactam and macrolide resistance genes, followed by aminoglycoside, sulphonamide, and tetracycline classes. Conclusions: These findings highlight urban wastewater as a relevant reservoir and dissemination route for AMR and support the integration of metagenomic approaches into wastewater surveillance programs. By providing region-specific, integrated taxonomic, functional, and resistome data from Sicilian WWTPs, this study contributes to the growing body of evidence supporting WBE as a valuable tool for AMR monitoring and One Health-oriented risk assessment. Full article

Problem Statement: Two global trends, including aging populations and the acceleration of global warming, are increasing the risk of heat-related illness, challenging the health of populations, and the sustainability of healthcare systems. Global warming refers to the increase in the Earth’s average surface temperature, generally attributed to the greenhouse effect, which is occurring at three times the rate of the pre-industrial era. The global population of older adults, defined here as individuals aged 60 and over, is expected to reach over 2 billion by mid-century. This population is particularly vulnerable to heat-related illness, specifically disruption of thermoregulation from excessive exposure to environmental heat due to metabolic and cognitive changes associated with aging. Objectives: This review examines heat-related illness and its impact on older adults within a socio-ecological framework, considering both drivers and mitigation strategies related to global warming, the built environment, social determinants of health, healthcare system responses, and the individual. The authors were motivated to create a conceptual model within this framework drawing on their lived experiences as healthcare providers interacting with older adults in a large urban area of the southwestern US, known for its extreme heat and extensive heat island effects. Based on this framework, the authors suggest actionable strategies supported by the literature to reduce the risks of morbidity and mortality. Methods: The literature search utilized a wide lens to identify evidence supporting various aspects of the hypothesized framework. In this sense, this review differs from systematic and scoping reviews, which seek a complete synthesis of the available literature or a mapping of the evidence. The first author conducted the literature search and synthesis, while the second and third authors reviewed and added publications to the initial search and conceptualized the socio-ecological framework. Discussion: This study is unique in its focus on a global trend that threatens the well-being of a growing population. The population health focus underscores social determinants of health and limitations of existing healthcare systems to guide healthcare providers in reducing older adults’ vulnerability to heat-related illness. This includes patient education regarding age-related declines in extreme heat tolerance, safe and unsafe physical activity habits, the impact of prescription drugs on heat tolerance, and, importantly, identifying the symptoms of heatstroke, which is a medical emergency. Additional strategies for improving survivability and quality of life for this vulnerable population include improved emergency response systems, better social support, and closer attention to evidence-based treatment for heat-related health conditions. Full article

Non-communicable diseases, particularly cardiovascular diseases (CVD) and metabolic syndrome (MS), remain a major challenge for primary health care (PHC). This study aimed to assess cardiometabolic risk and health behaviours in adult PHC patients using routine preventive screening. This prospective observational study included 506 adults attending routine consultations in an urban PHC centre in Poland. Preventive assessment included anthropometric measurements (body weight, height, BMI, and waist circumference), blood pressure, lipid profile, and fasting glucose levels. Health behaviours were recorded using the standardised NFZ CHUK questionnaire. The 10-year CVD risk was estimated using the SCORE2 algorithm. Multivariable logistic regression was used to identify independent factors associated with high cardiovascular risk (SCORE2 ≥ 5%) and of a composite endpoint defined as the presence of any non-optimal biochemical parameter. Nearly half of the participants had excess body weight (overweight or obesity), and more than half met criteria for central obesity. Borderline or elevated total cholesterol was found in 47% of patients, abnormal LDL in 27%, low HDL-C (<40 mg/dL) in 80% (84% when applying sex-specific cut-offs), and impaired fasting glucose or diabetes in about 12%. High SCORE2 risk (≥5%) was observed in approximately 9% of the cohort. In multivariable models, SCORE2 components (age, sex, and smoking) were, as expected, associated with high SCORE2 risk, and obesity (BMI ≥ 30 kg/m²)—a factor not included in SCORE2—was additionally associated with higher risk. Additionally, age, male sex, and obesity also predicted the presence of at least one non-optimal biochemical marker. The prevalence of high SCORE2 risk increased from 1.2% in patients with 0–1 modifiable risk factor to 25.7% in those with 4–5 factors. Lower educational attainment was associated with a higher proportion of high-risk individuals in univariate analysis. Routine preventive activities in PHC enable the identification of important lipid and glucose abnormalities and the clustering of modifiable risk factors, even in a relatively young, highly educated population. Systematic cardiovascular screening and a focus on patients with accumulated risk factors should remain a priority in PHC to enable early identification of high-risk patients and timely implementation of lifestyle and therapeutic interventions. Full article

Urban shared parking systems represent a complex socio-technical challenge. Despite vast potential, utilization remains persistently low (<15%), revealing a critical policy failure. To address this, this study develops a dynamic system framework based on Life-Cycle Cost (LCC) and Hamilton-Jacobi-Bellman (HJB) optimization to analyze and calibrate the key policy levers influencing owner participation timing (T*). The model, resolved using finite difference methods, captures the system’s non-linear threshold effects by simulating critical system parameters, including system instability (price volatility, ${\mathsf{\sigma }}_p$), internal friction (management fee, $w_g{g}_t$), and demand signals (transaction ratio, Q). Simulations reveal extreme non-linear system responses: a 100% increase in system instability (${\mathsf{\sigma }}_p$) delays participation by 325.5%. More critically, a 100% surge in internal friction (management fees) delays T* by 492% and triggers a 95% revenue collapse—demonstrating the risk of systemic collapse. Conversely, a 20% rise in the demand signal (Q) advances T* by 100% (immediate participation), indicating the system can be rapidly shifted to a new equilibrium by activating positive feedback loops. These findings support a sequenced calibration strategy: regulators must first manage instability via price stabilization, then counteract high friction with subsidies (e.g., 60%), and amplify demand loops. The LCC framework provides a novel dynamic decision support system for calibrating complex urban transportation systems, offering policymakers a tool for scenario testing to accelerate policy adoption and alleviate urban congestion. Full article

Background/Objectives: The Lancet Commission proposes a new obesity definition that combines body mass index (BMI) with anthropometric measurements to distinguish adipose tissue excess more effectively. This study aims to determine the prevalence of obesity based on the new definition and to examine cardiometabolic risk factors and lifestyle habits across different obesity phenotypes in the urban population of Lithuania. Methods: This study was conducted among residents of Kaunas city from 2020 to 2024. A total of 3426 adults aged 25–69 years (57.1% of the random sample) were participated. Three individuals were excluded due to missing anthropometric data. Participants were categorized into three phenotypes: (1) no obesity (BMI < 30 kg/m² and no or one elevated anthropometric measure, (2) anthropometric-only obesity (BMI < 30 kg/m² and at least 2 elevated anthropometric measures), and (3) BMI-plus-anthropometric obesity (BMI ≥ 30 kg/m² plus at least one elevated anthropometric measure or BMI ≥ 40 kg/m²). Standardized anthropometric, biochemical, and clinical measurements were collected, along with self-reported dietary habits and leisure-time physical activity. Results: Anthropometric-only obesity was highly prevalent, affecting 36.1% of males and 22.7% of females (p < 0.05). The prevalence of BMI-plus-anthropometric obesity was 24.1% among males and 21.4% among females. Individuals with anthropometric-only obesity had significantly higher odds of metabolic syndrome (OR 8.64; 95% CI 6.97–10.71), diabetes (OR 3.01; 95% CI 1.72–5.25), coronary heart disease (OR 1.48; 95% CI 1.12–1.97), and several lipid abnormalities compared with those without obesity. The highest cardiometabolic risk was observed in the BMI-plus-anthropometric obesity group. Greater adiposity was associated with higher intake of red meat, junk foods, and sugary drinks, while physical activity levels declined across obesity categories. Conclusions: Anthropometric-only obesity is a common and metabolically adverse phenotype that cannot be detected using BMI alone. A new obesity definition enhances identification of high-risk individuals and supports targeted prevention strategies. Full article

Background: Herpes zoster (HZ) represents a substantial public health concern among aging populations, yet regional variability in clinical patterns and risk determinants remains insufficiently documented. In southeastern Romania, epidemiological data are limited, and the combined influence of demographic, behavioral, and metabolic factors on disease severity has not been systematically evaluated. Methods: We performed a retrospective observational study including 100 consecutive patients diagnosed with HZ between 2019 and 2023 in a dermatology department in southeastern Romania. Demographic characteristics, lifestyle behaviors, anthropometric status, clinical manifestations, and outcomes were extracted from medical records. Associations between categorical variables were assessed using Chi-square tests and Cramer’s V, while interaction patterns were explored through log-linear modeling. Heatmaps were generated in Python (version 3.10) using the Matplotlib library (version 3.7.1) to visualize distribution patterns and subgroup relationships. Results: The cohort showed a marked age dependence, with 77% of cases occurring in individuals ≥ 60 years, consistent with immunosenescence-driven reactivation. Women represented 59% of cases, and 84.7% of female patients were postmenopausal. Urban residents predominated (91%). Vesicular eruption (84%) and acute pain (79%) were the most frequent symptoms. Localized HZ was observed in 81% of cases, while ophthalmic involvement (11%) and disseminated forms (8%) were less common. Lifestyle factors significantly influenced clinical severity: smokers, alcohol consumers, and sedentary individuals exhibited higher proportions of postherpetic neuralgia (PHN) and ocular complications (p < 0.001). Overweight and obese patients demonstrated a higher burden of PHN, suggesting a role for metabolic inflammation, although BMI was not associated with incidence. No significant association between age category and complication type was detected, likely due to small subgroup sizes despite a clear descriptive trend toward increased severity with advanced age. Conclusions: These findings support a multifactorial model of HZ severity in southeastern Romania, shaped by age, lifestyle behaviors, hormonal status, and metabolic risk. While incidence patterns align with international data, the strong impact of modifiable factors on complication rates highlights the need for targeted prevention and individualized risk assessment. Results offer a regional perspective that may inform future multicenter investigations. Full article

This paper presents the results of an integrated research and design process developed within the Master’s study programme in Architecture at the University of Belgrade—Faculty of Architecture, aimed at exploring architectural agency in conditions of ecological degradation, declining biodiversity, and the urgent need for regenerative transformation of the built environment. Moving beyond technologically driven notions of “green design,” the study investigates architectural approaches that support ecosystem restoration, biodiversity enhancement, and multispecies coexistence while strengthening health and well-being. Grounded in a three-phase methodological framework, the research (1) formulates conceptual models of therapeutic landscapes through typo-morphological, place-based, and adventure-based analytical approaches; (2) evaluates these models using the New European Bauhaus (NEB) Checklist to assess their alignment with the core values of sustainability, beauty, and togetherness; and (3) synthesizes the findings into regenerative design scenarios that integrate ecological processes, multisensory experience, and community participation. The results position therapeutic landscapes as a spatial practice in which architecture functions as ecological infrastructure, a metabolic system where natural cycles, cultural meanings, bodily experiences, and more-than-human agencies interact. In this sense, architectural design becomes the basis for re-naturalization, regeneration, ecological care, multisensory experience, and resilience in urban, peri-urban, and rural communities. Full article

Concrete is the most widely used construction material worldwide, yet its production and disposal pose significant environmental challenges due to high carbon emissions and limited recyclability. While microbial colonization of concrete is often associated with structural deterioration, recent research has highlighted the potential of microorganisms to contribute positively to concrete recycling and self-healing. In this study, we investigated the bacterial and fungal communities inhabiting urban concrete samples using amplicon-based taxonomic profiling targeting the 16S rRNA gene and internal transcribed spacer (ITS) region. Our analyses revealed a diverse assemblage of microbial taxa capable of surviving the extreme physicochemical conditions of concrete. Several taxa were associated with known metabolic functions relevant to concrete degradation, such as acid and sulphate production, as well as biomineralization processes that may support crack repair and surface sealing. These findings suggest that concrete-associated microbiomes may serve as a reservoir of biological functions with potential applications in sustainable construction, including targeted biodegradation for recycling and biogenic mineral formation for structural healing. This work provides a foundation for developing microbial solutions to reduce the environmental footprint of concrete infrastructure. Full article

This study explores global experiences of capital relocation to extract lessons relevant to North Maluku, Indonesia, where Sofifi has yet to develop into a functional provincial capital. Drawing on six theoretical perspectives, including growth pole theory, polycentric development, institutional inertia, urban metabolism, spatial justice, and urban vitality, the paper analyzes how political vision, institutional integration, and social participation influence relocation outcomes. Comparative cases from Abuja, Brasília, Putrajaya, Naypyidaw, Randstad, and Nusantara show that successful relocations occur when governance reform aligns with spatial planning and participatory urban design. For Sofifi, enhancing urban vitality through connectivity, inclusiveness, and institutional coordination is essential to transform relocation from a symbolic decision into a functional urban system. The study contributes a conceptual framework linking spatial design, institutional reform, and social vibrancy in the pursuit of sustainable and equitable capital development. Full article

Urban areas often suffer from enduring environmental issues, including flooding, biodiversity loss, heat island effects, and air and soil pollution. The Miyawaki method of afforestation, characterized by dense planting of native species on remediated soil, has been proposed as a rapid, nature-based solution for restoring urban ecological function. This study aims to evaluate early-stage changes in soil health following Miyawaki-style microforest establishment in formerly redlined neighborhoods in Elizabeth, New Jersey. Specifically, it investigates whether this method improves soil permeability, carbon content, and microbial activity within the first three years of planting. Three microforests aged one, two, and three years were assessed using a chronosequence approach. At each site, soil samples from within the microforest and adjacent untreated urban soil (control) were compared. Analyses included physical (porosity, dry density, void ratio), chemical (total carbon), and biological (microbial respiration, biomass, metabolic rate, carbon use efficiency) assessments. Soil permeability was estimated via the Kozeny–Carman equation. Microforest soils showed significantly greater porosity (p = 0.015), higher void ratios (p = 0.009), and reduced compaction compared to controls. Soil permeability improved dramatically, with factors ranging from 5.99 to 52.27. Total carbon content increased with forest age, reaching 2.0 mg C/g in the oldest site (p < 0.001). Microbial metabolic rate rose by up to 287.5% (p = 0.009), while carbon use efficiency also improved, particularly in the older microforests. Within just one to three years, Miyawaki microforests significantly enhanced both the physical and biological properties of degraded urban soils, signaling rapid restoration of soil function and the early return of ecosystem services. Full article

Microplastics represent an emerging threat to aquatic environments and organisms, as they infiltrate water systems, are ingested by marine species, and cause physical harm, endocrine disruption, and bioaccumulation up the food chain, potentially impacting biodiversity and human health. Aquatic ecosystems face considerable harm from microplastic pollution because fish in the early developmental stages, including embryos, larvae, and juveniles, are more susceptible due to their immature physiological and detoxification systems. This review aims to comprehensively explore the impacts of microplastics on the early life stages of fish. Aquatic environments receive primary and secondary MPs from urban runoff and industrial waste, together with degraded plastics, which affect fish embryos and larvae via direct ingestion, surface adhesion, and trophic transmission pathways. The physical impact of MPs causes digestive tract blockages that reduce hatching success and create developmental problems in fish organs, but chemical toxicity develops from plasticizers, heavy metal leaching, and pollutant adsorption, which causes oxidative stress, endocrine disruption, and metabolic dysfunction. Survival rates decrease because exposure causes fish to perform poorly during swimming activities and make limited efforts to avoid predators. The small dimensions and high chemical reactivity of MPs increase their bioavailability, which promotes tissue penetration and leads to accumulation at different levels of the food chain. This comprehensive review emphasizes that we need to establish uniform detection protocols, long-term exposure research, and effective strategies to control MP pollution. The resolution of these difficulties remains essential for protecting fish populations, as well as for protecting biodiversity and minimizing seafood contamination risks to human health. Full article

The gut microbiota plays an essential role in human health through its contributions to immune regulation, metabolism, pathogen defence and disease susceptibility. Despite this significance, most gut microbiome research remains disproportionately focused on high-income countries, resulting in a limited and underrepresented view of global microbial diversity. This bias is evident in Africa, where populations, including those in South Africa, show unique combinations of genetic variation, dietary patterns and environmental exposures that are insufficiently captured in current datasets but offer opportunities to uncover novel insights into microbial evolution and its influences on health across diverse settings. In response to this gap, this review synthesises global patterns in gut microbiota composition and diversity while situating South African findings within this broader context. We examine evidence across microbial domains, including bacteria, fungi, viruses, archaea, protozoa and helminths, and highlight the impact of dietary transitions and environmental exposures on microbial community structure. Although still emerging, research on the gut microbiome of South African populations consistently reports contrasts between rural and urban populations, with rural groups enriched in fibre-fermenting and anti-inflammatory taxa, whereas urban communities often exhibit reduced diversity and features of dysbiosis linked to Westernisation. However, limited sample sizes, heterogeneous methodologies and absence of multi-omic approaches constrain robust interpretation. These lacunae in current knowledge emphasise the urgent need for large-scale, longitudinal studies that reflect South Africa’s demographic and geographic diversity. Strengthening this evidence will not only help identify microbial signatures linked to modifiable lifestyle factors but will also guide nutrition, prevention and screening programmes to improve health in African populations. Full article

Groundwater is increasingly relied upon in cities, particularly during drought, yet its management often lacks coordination and systems-based decision-making. Effective governance requires inclusive participation across sectors and scales, engaging actors with diverse knowledge, experiences, and priorities. In cities, this is challenging due to the wide range of roles and responsibilities tied to groundwater. This study examines the value of urban water metabolism analysis (UWMA) for enhancing groundwater governance in Cape Town and Nelson Mandela Bay, South Africa—both recently affected by severe drought. Through a series of Learning Labs, we convened groundwater-related actors to co-develop a shared understanding of urban water systems. We brought together two methods of systems enquiry, UWMA and governance network analysis to explore physical stocks and flows of water across metropolitan boundaries with governance processes shaping groundwater management. The UWMA revealed that, prior to the 2015 drought, Nelson Mandela Bay’s water supplies were more diversified than those of Cape Town, despite Cape Town progressively pursuing managed aquifer recharge and wastewater reuse. The governance analysis surfaced the diversity of actors influencing groundwater flows across the public, private, and civil society sectors, yet highlighted the fragmented nature of the network, with geohydrology and engineering consultants often acting as intermediaries. This research found that UWMA was perceived to be most useful at larger scales (e.g., watershed/urban scales) and was considered a valuable tool for strategic discussion, though clearer language would increase accessibility. We conclude that UWMA helps identify knowledge gaps, integrate diverse perspectives, and foster stakeholder cooperation. Coupled with scenario planning, it can support participatory and inclusive decision-making. Full article

Urban trees are vital for air pollution mitigation, but their function is often compromised by exposure to particulate matter (PM), which impairs physiological processes and reduces growth. Enhancing tree resilience is therefore essential for maintaining their ecosystem services in polluted urban environments. This study examined the early growth and biochemical responses of Norway spruce (Picea abies) and Norway maple (Acer platanoides) seedlings to foliar PM exposure and assessed whether biochar (BC) soil amendment can alleviate PM-induced stress. Seedlings were cultivated outdoors under three treatments: Control (no treatment), PM (foliar exposure to particulate matter), and PM + BC (PM exposure with 10% biochar added to the substrate). Results revealed that Norway maple showed significant biochemical sensitivity to PM, including substantial reductions in chlorophyll and increases in antioxidant activity. However, Norway spruce showed more moderate pigment changes but reduced height growth. BC modulated oxidative and phenolic responses (TPC, TFC, MDA) and partially mitigated PM-induced stress, although its effectiveness varied by species. For Norway spruce, BC significantly enhanced resilience by restoring height growth, stabilizing pigments, and reducing oxidative stress compared with treatment using PM alone. In contrast, for Norway maple, BC failed to restore chlorophyll levels and increased oxidative and phenolic activity, yielding mixed outcomes. Despite physiological differences between the two species, multivariate PCA consistently showed that PM-treated seedlings diverged from the control cluster, whereas PM + BC-treated seedlings were closer to the controls, with mitigation substantially stronger in Norway spruce. These findings demonstrate that biochar can reduce PM-induced stress, but its successful implementation depends fundamentally on selecting appropriate species traits and understanding their specific metabolic response strategies. Full article

Understanding the seasonal dynamics of phytochemical composition in evergreen species is crucial for improving ecosystem productivity models and selecting appropriate species for urban landscapes under changing climate conditions. However, knowledge about how light environment, temperature, and precipitation interact to regulate leaf biochemical processes across seasons remains limited. We investigated morphological and biochemical responses of cherry laurel (Prunus laurocerasus L.) grown under contrasting light environments (light-exposed versus shaded) across twelve months, analyzing photosynthetic pigments, antioxidants, osmolytes, and secondary metabolites in relation to environmental variables. Light-exposed leaves exhibited enhanced accumulation of photoprotective compounds, including carotenoids (9.38 mg g⁻¹), xanthophylls (3.60 mg g⁻¹), and flavonoids (0.51 mg g⁻¹), along with superior total antioxidant capacity during spring and autumn. Proline showed bimodal seasonal peaks (93.7 µmol g⁻¹ in August under shade, 71.1 µmol g⁻¹ in July under light), indicating stress responses to both summer heat and winter cold. Multivariate analyses revealed that seasonal variation accounted for 94.9% of total phytochemical variability, with distinct metabolic signatures characterizing winter (high glycine betaine, anthocyanin), spring (high chlorophyll, phenolics), summer (high proline, transient carotenoid peaks), and autumn (maximum antioxidant capacity) periods. We conclude that light environment significantly influences cherry laurel’s seasonal metabolic strategies, with shade-grown plants prioritizing light harvesting efficiency and osmotic adjustment, while light-exposed plants emphasize photoprotection and antioxidant defense. The coordinated regulation of functionally related compounds reveals integrated stress response mechanisms that contribute to cherry laurel’s remarkable environmental plasticity. These quantitative seasonal patterns provide valuable parameters for optimizing cultivation practices, predicting biochemical composition for harvesting purposes, and modeling the ecological performance of this species in variable urban and forest environments under climate change scenarios. Full article