Search Results (197)

Fungal laccases are promising oxidative enzymes for bioremediation applications, particularly in the degradation of synthetic dyes present in industrial effluents. Here, we evaluated the inhibitory effects of sodium chloride (NaCl) and sodium sulfate (Na₂SO₄) on the activity of Trametes versicolor laccase and its ability to decolorize Congo Red (CR), Malachite Green (MG), and Remazol Brilliant Blue R (RBBR). Enzyme assays revealed concentration-dependent inhibition, with IC₅₀ values of 0.22 ± 0.04 M for NaCl and 1.00 ± 0.09 M for Na₂SO₄, indicating stronger inhibition by chloride. Kinetic modeling showed mixed-type inhibition for both salts. Despite this effect, the enzyme maintained significant activity: after 12 h, decolorization efficiencies reached 95 ± 4.0% for MG, 88 ± 3.0% for RBBR, and 75 ± 3.0% for CR, even in the presence of 0.5 M salts. When applied to a mixture of the three dyes, decolorization decreased only slightly in saline medium (94.04 ± 4.0% to 83.43 ± 5.1%). FTIR spectra revealed minor structural changes, but toxicity assays confirmed marked detoxification, with radicle length in lettuce seeds increasing from 20–38 mm (untreated dyes) to 41–48 mm after enzymatic treatment. Fungal growth assays corroborated reduced toxicity of treated dyes. These findings demonstrate that T. versicolor laccase retains functional robustness under ionic stress, supporting its potential application in saline textile wastewater remediation. Full article

A mycelium is a network of hyphae that possesses the ability to self-assemble and grow into various shapes, acting as a natural binder that minimises the need for intensive chemical and energy processes, making it an alternative capable of forming structures that may eventually outperform traditional fibres such as animal leather and polyester. In this work, two mycelium mats were created, and their thickness, water absorption, coverage, and tear strength for the sewing process were determined. Fibre mats were grown in vitro or on a jute substrate. The mats were treated with salt, tannin or citric acid solutions, then air- or oven-dried. In general, the treatment that least modified the colour and appearance of the mycelium mats was citric acid, and when dried by airflow, the thickness averaged 1.4 mm. The highest tear strengths were 10.55 N/mm and 12.7 N/mm for the mycelium mats treated with citric acid without and with jute, respectively. A high percentage of water absorption was observed, reaching 267% (mycelium mats treated with tannins and dried at 65 °C) and 28% (mycelium mats treated with citric acid and air-dried). In general, all mycelium mats can be sewn, except for those treated with citric acid, which have a viscous texture and require slow sewing to prevent the mycelium from breaking. The Trametes fungus can be utilised in the production of mycelial materials, allowing for the optimisation of growth conditions to obtain mycelial mats that meet the requirements for use as an environmentally friendly alternative in the textile and related industries. Full article

Evaluating wood’s natural durability is essential when establishing the level of protection that is required depending on the end use to extend its service life. Natural durability is defined as the intrinsic resistance of wood against the attack of destructive organisms. There are standardized methods for estimating a durability value. In Europe, the EN 350:2016 standard is responsible for establishing the basic guidelines, as well as the necessary tests to determine this value. This standard applies to commercial wood, both native and imported, and helps to improve and obtain better construction applications depending on the final use. This work aimed to study the natural durability of Quercus pyrenaica Wild. against Basidiomycetes (Coniophora puteana (Schumacher ex Fries) Karsten and Trametes versicolor (Linnaeus) Quélet) and termites (Reticulitermes grassei Clément). The standards applied were EN 113-2:2021 and EN 117:2023. The heartwood of the Quercus pyrenaica is highly durable against both xylophages basidiomycetes and subterranean termites. The sapwood is moderately durable against Coniophora puteana, slightly durable-not durable against Trametes versicolor, and moderately durable against termites. These results open the door for commercialization of this species, and it is expected to be included in EN 350:2016, where Q. pyrenaica is not included. Full article

Birch stands, dominated by Betula pendula Roth, are a common feature of boreal forests. Within the Krasnoyarsk (central) group of regions, they are concentrated in the taiga, subtaiga and forest steppe zones of actively managed forests, represented by stands of seed and shoot origin. The health and productivity of birch forests is often determined by the activity of wood-decay fungi, which leads to rot and decay in trees. The objective of the research is to evaluate the impact of stem rot on birch forests in the study area, with a focus on key ecological and silvicultural factors. The research methods employed included a reconnaissance survey of birch forests, a detailed forest pathology survey of forest stands on research plots (31 pcs.), comprehensive macroscopic diagnostics of stem rot, identification of xylotrophic fungi by basidiomes, integrated assessment of forest health, graph analytics and statistical data analysis. Stem rot has been identified in all birch forests in the study area. In shoot origin stands, the incidence rate has reached the stage of the disease center (i.e., more than 10% of trees are infected). The following wood-decay fungi have been detected on the trunks of living trees affected by rot: Fomes fomentarius, Fomitopsis pinicola, Inonotus obliquus, Phellinus igniarius, and Trametes versicolor. The infection typically infects trees via spores, finding entry through dying branches or mechanical and thermal wounds on trunks. In trees of shoot origin, stem rot is frequently transmitted via mycelium from stumps left after felling. This, in conjunction with diminished immunity, contributes to a substantially elevated incidence of stem rot in comparison to stands of seed origin. The research has not established a reliable correlation between the incidence of stem rot and forest stand characteristics due to the impact of human activity on birch forests (e.g., cutting, fires, tree injury). At the same time, no reliable connection has been established between the spread of stem rot and the stage of recreational disturbance. Trees of various sizes are affected by stem rot, usually proportional to their representation in the structure of the forest stand. The disease has a detrimental effect on the trees, which is clearly evident in the decline of forest health. Full article

Mycelium-based biocomposites (MBBs) represent a sustainable alternative to synthetic composites, as they are produced from lignocellulosic substrates bonded by fungal mycelium. Their mechanical performance depends on multiple interacting factors, including the substrate composition, fungal species, and processing conditions, which makes property optimisation challenging. In this study, an artificial neural network (ANN) model was developed to predict two mechanical properties of MBBs, namely internal bonding (IB) and compressive strength (CS). An ANN model was trained on experimental data, using the substrate composition, fungal species, and physical properties of MBBs. The ANN predictions were compared with measured values, and the model accuracy was evaluated. The results showed that the ANN achieved a high predictive accuracy, with coefficients of determination of 0.992 for IB and 0.979 for CS. IB values were predicted more precisely than CS, likely due to microstructural heterogeneities. The heterogeneities were visualised using scanning electron microscopy. Composites produced with Ganoderma sessile and Trametes versicolor exhibited the highest IB. Interestingly, Trametes versicolor achieved the highest CS on virgin wood particles but the lowest values on recycled wood, underlining the strong influence of the substrate quality. The study demonstrates that ANNs can effectively predict the mechanical properties, reducing the number of experimental tests needed for material characterisation. Full article

The antifungal effect of catechin and extractives from Prosopis juliflora was studied against one white rot fungus, Trametes versicolor (TV), and one brown rot fungus, Poria placenta (PP). The mentioned extractives from Prosopis julilfora were crude mesquitol and pure mesquitol. Tebuconazole was used in this study as a known fungicide against the two named fungi. Wood preservation using the current synthetic fungicides can be harmful to the environment and toxic to animals and plants. To help solve these problems, fungicides can be mixed with natural extractives to act synergistically as wood preservatives. Most of these natural extractives contain polyphenols, which are secondary metabolites, having good antioxidant properties, which may inhibit radical species involved in wood cell polymer defects. In this study, 1000 ppm and 5000 ppm of crude mesquitol, pure mesquitol and catechin had a very good growth inhibition against TV and PP. Thus, the concentrations were used to assess their synergistic response when mixed with lower inhibitory concentration of tebuconazole. The results showed that there was an additive effect in a combination of 0.1 ppm tebuconazole with 1000 ppm pure mesquitol for PP, 0.5 ppm Tebuconazole with 1000 ppm crude mesquitol and pure mesquitol for PP and 0.5 ppm tebuconazole with 1000 ppm pure mesquitol for TV. The other remaining combinations of 1000 ppm/5000 ppm of the samples with 0.1 ppm/0.5 ppm tebuconazole all had synergistic effect. This data suggests that a combination of polyphenols (catechin and extractives) with tebuconazoles can be useful sources for preparation of fungicides and wood preservatives for agricultural use and wood durability, respectively. Full article

The discharge of synthetic dyes from industries poses severe environmental challenges, necessitating eco-friendly remediation strategies. This study investigated the biosorption of triarylmethane dyes Crystal Violet (CV), and Brilliant Green (BG) using self-immobilized and sponge-immobilized biosorbents of Trametes versicolor (strain CB8, CB8/S2) and Pleurotus ostreatus (strain BWPH, BWPH/S2). Tests were conducted with live and autoclaved biomass under varying conditions of dye concentration (100–400 mg/L), temperature (15–55 °C), and pH (2–10). Sponge-immobilized live biomass (CB8/S2 and BWPH/S2) showed superior performance, removing up to 90.3% and 81.7% of BG and 43.9% and 39.3% of CV, respectively, within 6 h, demonstrating 3–5 times higher efficiency than self-immobilized biomass for both dyes. Maximum sorption of 379.4 mg/g of BG and 48.9 mg/g of CV was achieved by CB8/S2 at 400 mg/L. Principal Component Analysis biplot confirmed immobilization efficacy, where Dim1 (85.9–91.8% variance) dominated dye concentration and contact time. The optimized conditions for BG removal by CB8/S2 was 20.85–32.17 °C and pH 3.4–6, and for CV, at pH 6.5–7.5 and 30 °C. The percentage of dye sorption data fitted well with the quadratic model (p < 0.05). Fourier transform infrared spectroscopy (FT-IR) analysis indicated that hydrogen bonding and electrostatic interactions facilitated dye binding onto fungal mycelium. Notably, sponge-immobilized biosorbents were reusable without additional treatment. The findings support fungal biomass immobilization as a viable strategy to augment the bioremediation potential in treating dye-laden wastewater. Full article

Modern culture, strongly influenced by the growth of sectors such as the fashion and textile industries, has generated an environmental trend that is difficult to reverse. It is estimated that between 60 and 70% of the dyes used in these sectors are synthetic, which offer great versatility, a low cost, and a broad spectrum of colors, making them indispensable in many sectors. Among these synthetic dyes, azo dyes stand out due to their excellent chromophoric properties, structural stability, and ease of synthesis. However, these compounds are considered xenobiotics with a strong recalcitrant potential. This review article comprehensively examines the biodegradation potential of azo contaminants by microorganisms, including bacteria, fungi, microalgae, and consortia, using the PRISMA 2020 methodology. In this regard, this study identified 720 peer-reviewed articles on this topic that are outstanding. The analysis of these studies focused on the effect of parameters such as pH, temperature, and exposure time, as well as the enzymatic degradation pathways associated with the degradation efficiency of these contaminants. For example, the results identified that microorganisms such as Meyerozyma guilliermondii, Trametes versicolor, Pichia kudriavzevi, Chlorella vulgaris, and Candida tropicalis possess significant potential for degrading azo dyes (up to 90%). This degradative efficiency was attributed to the high enzymatic activity that cleaves the azo bonds of these contaminants through specialized enzymes, such as azoreductases, laccases, and peroxidases. Furthermore, the results highlight synergistic effects or metabolic cooperation between species that enhance the biodegradation of these contaminants, suggesting an eco-friendly alternative for environmental remediation. Full article

This paper reports on a preliminary experimental study on binder jetting 3D printing of biomass–fungi composite materials. Biomass–fungi composite materials have potential applications in the packaging, furniture, and construction industries. Biomass particles (prepared from agricultural residues) act as the substrate of the composite materials. The filamentous roots of fungi intertwine and bind biomass particles together. In this study, the biomass (hemp hurd) powders used had two distinct average particle sizes. The liquid binder used contained fungi (Trametes versicolor) cells. T-shaped samples were printed using a lab-designed binder jetting setup. Printed samples were kept inside an incubator oven for four days to allow fungi to grow. Afterward, loose biomass powder was removed from the T-shaped samples. The samples were then kept inside the incubator oven for eight more days to allow further fungal growth. The samples were subsequently placed in an oven at 120 °C for four hours to terminate all fungal activity in the samples. SEM micrographs were taken of the cross-sectional surfaces of the samples. The micrographs showed a significant presence of fungi hyphae inside the printed samples, providing evidence of the binding of biomass particles by the hyphae. Full article

Plastic is a major organic pollutant globally but has only recently been recognized for its recalcitrant nature and resistance to degradation. Although vast amounts of plastic debris are overwhelming the planet, the search for solutions to its degradation has only recently begun. One of the most well-known agents of plastic biodegradation is the larvae of Tenebrio molitor, which can alter the structure of polymers like polystyrene. However, while this insect can cause deterioration, its frass, which still consists of polystyrene microplastics, remains a problem. We investigated whether this frass could be further degraded by strains of white rot fungi, specifically Pleurotus eryngii and Trametes versicolor. We introduced two PS derivatives (styrofoam and stirodure) to the fungi in liquid media and evaluated oxidative metabolism enzymes (laccase, Mn-peroxidase, lignin-peroxidase) activities, and the phenolic products of the potential aromatic polymer degradation in the media. Finally, we evaluated FTIR spectra to determine if we could detect changes in polystyrene molecule degradation. Both fungi produced high amounts of enzymes, particularly when the polystyrene was present. Large quantities of phenolic substances were simultaneously detected, some associated with polystyrene degradation. FTIR spectra of different polystyrene products confirmed species-specific mechanisms for their degradation by experimental fungal strains. Full article

In Germany, Pinus sylvestris is a dominant tree species, and many trees with large diameters are not utilised due to difficulties with processing. However, older pines have larger volumes of sapwood, and boards with a high sapwood content can be produced. The durability of boards from large-diameter (>50 cm) P. sylvestris trees, treated with furfurylation, acetylation, DMDHEU (1.3-dimethylol-4.5-dihydroxyethyleneurea), and SorCA (Sorbitol/Citric Acid), was assessed. The samples were taken from different sections along the longitudinal axis and the cross-section. The durability was tested against Coniophora puteana, Rhodonia placenta, and Trametes versicolor, according to the EN 113-2 standard. All treatments had a median mass loss < 5%, so classed as “highly durable” (Durability Class 1) against all fungi. DMDHEU had a large deviation in mass loss against Coniophora puteana and could potentially be classified as “moderately durable” (Durability Class 3), if based on the mean mass loss. The inner part of the end section had a higher mass loss, indicating that there was poorer retention of the treatment at this location. Overall, chemical modifications on large-diameter pine trees were effective at increasing durability. Utilising large-diameter pine trees can help to make use of regional resources and potentially reduce reliance on imported timber. With favourable mechanical properties and easy-to-treat sapwood, large-diameter P. sylvestris trees could be used for commercial treatments. Full article

Nicotine, a major alkaloid in tobacco, poses significant environmental risks due to its persistence in wastewater. This study explores the degradation of naturally extracted nicotine from tobacco leaves by the white-rot fungus Trametes versicolor, aiming to assess its biodegradation capacity under diverse environmental stressors. Nicotine was extracted using a NaOH–petroleum ether method and confirmed through Fourier-transform infrared (FTIR) and nuclear magnetic resonance (NMR) spectroscopy. Biodegradation experiments were conducted using potato dextrose broth and synthetic wastewater as growth media under varying pH (2.5 and 5.20) and temperatures (25 °C and 37 °C). Fungal growth and nicotine degradation were monitored through biomass quantification and NMR-based analysis. Optimal degradation occurred at 25 °C and pH 5.20, particularly in synthetic wastewater, suggesting enhanced fungal adaptation in complex media. Degradation efficiency ranged from 80% to 99%, with synthetic wastewater outperforming conventional media. Extreme conditions, such as pH 2.5 at 37 °C, significantly hindered fungal growth. These findings demonstrate T. versicolor’s potential for effective nicotine removal in wastewater and highlight the environmental parameters critical to its performance. This work supports the development of sustainable fungal-based bioremediation strategies for managing nicotine contamination in aquatic environments. Full article

Persistent organohalogen pollutants—including halogenated nitrophenols (HNCs), trichloroethylene (TCE), and per- and polyfluoroalkyl substances (PFAS)—pose serious environmental and health risks due to their stability, toxicity, and bioaccumulation potential. This review critically assesses current remediation technologies including advanced oxidation processes (AOPs), adsorption, membrane filtration, and thermal treatments. While these methods can be effective, they are often limited by high costs, energy demands, toxic byproduct formation, and sustainability concerns. Emerging biological approaches offer promising alternatives. Among these, fungal-based degradation methods (mycodegradation) remain significantly underrepresented in the literature, despite fungi demonstrating a high tolerance to contaminants and the ability to degrade structurally complex compounds. Key findings reveal that white-rot fungi such as Phanerochaete chrysosporium and Trametes versicolor possess enzymatic systems capable of breaking down persistent organohalogens under conditions that inhibit bacterial activity. This review also identifies critical research gaps, including the need for direct comparative studies between fungal and bacterial systems. The findings suggest that integrating mycodegradation into broader treatment frameworks could enhance the environmental performance and reduce the long-term remediation costs. Overall, this review highlights the importance of diversifying remediation strategies to include scalable, low-impact biological methods for addressing the global challenge of organohalogen contamination. Full article

In recent years, the incidence rate of cancer has been on the rise all over the world, and the age of cancer patients has shown a younger trend, which seriously endangers patients’ health. Edible/medicinal mushrooms have not only become a new source of nutritional supplements but have also emerged as a promising adjunct to conventional medicine, either by directly or indirectly killing tumor cells and enhancing immunity, or through their use in conjunction with modern cancer therapies to enhance their efficacy or reduce their side-effects, improving patients’ quality of life. Although the anti-cancer potential of edible and medicinal mushrooms has been widely studied in the past, this review focuses on the most recent literature from the last five years, providing an up-to-date and comprehensive summary of the current findings. In this review, we aim to analyze the anti-cancer effects of edible/medicinal mushrooms, including Schizophyllum commune, Trametes versicolor, Grifola frondosa, Ganoderma lucidum, Lentinula edodes, Laetiporus sulphureus, Boletus edulis, and Phellinus igniarius, as well as their potential anti-cancer mechanisms, providing strong theoretical support for the further development of edible/medicinal mushroom anti-cancer products. Full article

This study investigates the coupled hygrothermal behavior of mycelium-based composites (MBCs) as a function of their microstructural organization, governed by fungal species, substrate type, additive incorporation, and treatment method. Eleven composite formulations were selected and characterized using a multi-scale experimental approach, combining scanning electron microscopy, dynamic vapor sorption, vapor permeability tests, capillary uptake measurements, and transient thermal conductivity analysis. SEM analysis revealed that Ganoderma lucidum forms dense and interconnected hyphal networks, whereas Trametes versicolor generates looser, localized structures. These morphological differences directly influence water vapor transport and heat conduction. Additive-enriched composites exhibited up to 21.8% higher moisture uptake at 90% RH, while straw-based composites demonstrated higher capillary uptake and free water saturation (up to 704 kg/m³), indicating enhanced moisture sensitivity. In contrast, hemp-based formulations with Ganoderma lucidum showed reduced sorption and vapor permeability due to limited pore interconnectivity. Thermal conductivity varied nonlinearly with temperature and moisture content. Fitting the experimental data with an exponential model revealed a moisture sensitivity coefficient thirty times lower for GHOP compared to VHOP, highlighting the stabilizing effect of a compact microstructure. The distinction between total and effective porosity emerged as a key factor in explaining discrepancies between apparent and functional moisture behavior. These findings demonstrate that hygric and thermal properties in MBCs are governed not by porosity alone, but by the geometry and connectivity of the internal fungal network. Optimizing these structural features enables fine control overheat and mass transfer, laying the groundwork for the development of high-performance, bio-based insulation materials. Full article