Search Results (140)

Plants are continuously exposed to multiple environmental stressors throughout their lifecycle. Understanding their integrated physiological, biochemical, and anatomical responses under combined stress conditions is crucial for developing effective approaches to improve stress tolerance and maintain crop productivity. This study aimed to investigate the physiological, biochemical, and anatomical changes in photomorphogenic Micro-Tom plants exposed to high irradiance and water deficit—an abiotic stress combination that commonly co-occurs in natural environments but remains poorly understood in light-sensitive genotypes. We hypothesized that the high pigment 1 (hp1) mutant, due to its enhanced light responsiveness, would display improved stress acclimation compared to the wild-type when exposed to combined stress factors. This study was conducted in a controlled plant growth chamber, using a randomized block design with five replicates. Two Micro-Tom genotypes (wt and hp1) were exposed to control (soil at field capacity (FC) + 450 μmol m⁻² s⁻¹ PPFD) and combined stress (40% FC + 1800 μmol m⁻² s⁻¹ PPFD) conditions. Despite the higher concentration of chloroplast pigments in hp1, its photosynthetic performance under combined stress was not significantly improved, and its defense mechanisms did not effectively mitigate the stress impacts. Anatomically, wt exhibited greater structural adjustment, observed by adaptations in the spongy parenchyma and mesophyll. Overall, the wt genotype showed stronger defense mechanisms, while hp1 was more susceptible to combined abiotic stress. Full article

Social-communicative abilities and theory of mind (ToM) are crucial for successful social interactions. The developmental trajectories of social and communicative skills characterizing individuals with Autism Spectrum Disorder (ASD) are rather complex and multidimensional, including components related to theory of mind. Due to its mentalistic nature, theory of mind has been rarely addressed as an outcome for Applied Behavior Analysis (ABA) intervention in children and adolescents with ASD. However, there is evidence that ABA intervention might be effective in promoting social abilities in individuals with ASD. Thus, this topic is worth investigating. We present a systematic review to explore the Ins and Outs of an ABA approach to promote social and communicative abilities and ToM in children and adolescents with ASD. We applied a PRISMA checklist to consider studies published up to December 2024. The keywords that we used were ToM, perspective-taking, false belief, social cognition, and mental states, in combination with ABA intervention and ASD (up to age 18). We searched for studies using Scopus, Google Scholar, and Medline. We included twenty studies on perspective-taking, identifying emotions, helping, detecting eye gazing, and social engagement, reviewing fifteen dedicated to teaching the interpretation of mental states (involving 49 children and 10 adolescents). The ToM was addressed with a multiple baseline design on target behaviors associated with ToM components such as identifying emotion, helping behaviors, and mental states. The intervention included a behavioral package consisting of Behavioral Skill Training, Derived Relations, video modeling, and role playing. The results indicated a significant number of participants who followed ABA intervention to promote social abilities and mastered the target behavior in ToM tasks; however, they showed maintenance and generalization issues across trials and settings. The role of predictors was highlighted. However, the studies are still rare and exhibit specific methodological limitations, as well as some clinical and ethical considerations. More research is needed to define best practices in ABA intervention to promote social abilities in individuals with ASD. Full article

Understanding others’ perspectives, or Theory of Mind (ToM), is a critical cognitive skill essential for social competence and effective interpersonal interactions. Although ToM is present in varying degrees across individuals, recent research indicates that linguistic factors, particularly bilingualism, can significantly influence its expression. Building on these findings, the current study examined performance on the perspective-taking trials of the Director Task among bilinguals and monolinguals. The results showed a nonsignificant trend in accurate responses as a function of bilingualism; however, a significant effect was found when examining only perspective-taking trials, with bilinguals outperforming monolinguals, suggesting that larger sample sizes are needed to identify this effect. Interestingly, a significant interaction between fluid intelligence and bilingualism was found, suggesting that bilinguals with higher fluid intelligence performed better on perspective-taking trials compared to bilinguals with lower fluid intelligence. The results emphasize the importance of domain-general abilities for the effect of bilingualism on perspective-taking and suggest that bilingualism’s effect on ToM may be more salient in individuals with higher cognitive abilities. Full article

This article investigates the effectiveness of using anti-turbulence additives (ATAs) to reduce hydraulic resistance during the pipeline transportation of high-viscosity oil. This study focuses on analyzing the impact of ATA dosage on the pressure loss and oil flow rate under turbulent flow conditions. Experiments demonstrated that a 40 g/t ATA concentration reduces hydraulic resistance by 25.1–36.7%, increasing pipeline throughput by ~60% (from 179.9 t/h to 289.8 t/h). This efficiency eliminates the need for constructing additional pumping stations, reducing capital costs by an estimated USD 5–7 million per 100 km of pipeline. Graphs confirm a nonlinear relationship between ATA dosage and pressure loss reduction, with optimal efficiency observed at 40 g/t. The findings emphasize the need for developing a comprehensive model that accounts for the physicochemical properties of the oil and additives, as well as the influence of flow parameters. These results provide a foundation for further research and the industrial application of ATA. Full article

Background: Tomatoes are self-pollinating plants, and successful fruit set depends on the production of functional pollen within the same flower. Our previous studies have shown that the ‘Black Vernissage’ tomato variety exhibits greater resilience to heat stress in terms of pollen productivity compared to the ‘Micro-Tom’ variety. Pollen productivity is determined by meiotic activity during microsporogenesis and the development of free microspores during gametogenesis. This study focused on identifying heat stress (HS)-induced proteomes in pollen mother cells (PMCs) and microspores. Methods: Tomato plants were grown under two temperature conditions: 26 °C (non-heat-treated control) and 37 °C (heat-treated). Homogeneous cell samples of meiotic PMCs (prior to the tetrad stage) and free microspores were collected using laser capture microdissection (LCM). The heat-induced proteomes were identified using tandem mass tag (TMT)–quantitative proteomics analysis. Results: The enrichment of the meiotic cell cycle in PMCs and the pre-mitotic process in free microspores confirmed the correlation between proteome expression and developmental stage. Under HS, PMCs in both tomato varieties were enriched with heat shock proteins (HSPs). However, the ‘Black Vernissage’ variety exhibited a greater diversity of HSP species and a higher level of enrichment compared to the ‘Micro-Tom’ variety. Additionally, several proteins involved in gene expression and protein translation were downregulated in PMCs and microspores of both varieties. In the PMC proteomes, the relative abundance of proteins showed no significant differences between the two varieties under normal conditions, with very few exceptions. However, HS induced significant differential expression both within and between the varieties. More importantly, these heat-induced differentially abundant proteins (DAPs) in PMCs are directly involved in meiotic cell division, including the meiosis-specific protein ASY3 (Solyc01g079080), the cell division protein kinase 2 (Solyc11g070140), COP9 signalosome complex subunit 1 (Solyc01g091650), the kinetochore protein ndc80 (Solyc01g104570), MORC family CW-type zinc finger 3 (Solyc02g084700), and several HSPs that function in protecting the fidelity of the meiotic processes, including the DNAJ chaperone (Solyc04g009770, Solyc05g055160), chaperone protein htpG (Solyc04g081570), and class I and class II HSPs. In the microspores, most of the HS-induced DAPs were consistently observed across both varieties, with only a few proteins showing significant differences between them under heat stress. These HS-induced DAPs include proteases, antioxidant proteins, and proteins related to cell wall remodeling and the generation of pollen exine. Conclusions: HS induced more dynamic proteomic changes in meiotic PMCs compared to microspores, and the inter-varietal differences in the PMC proteomes align with the effects of HS on pollen productivity observed in the two varieties. This research highlights the importance of the cell-type-specific proteomics approach in identifying the molecular mechanisms that are critical for the pollen developmental process under elevated temperature conditions. Full article

Research exploring involvement of RNA N⁶-methyladenosine (m⁶A) in tomato (Solanum lycopersicum) seed germination remains limited. There is also a lack of direct evidence supporting the interaction among tomato seed germination, microgravity, and m⁶A modification. In this study, Micro-Tom tomatoes are used as the experimental material to conduct tomato genetic transformation, seed germination assay, and m⁶A modification levels identification experiments. During tomato seed germination processes, the m⁶A modification level significantly increases under the mutual influence of various m⁶A methyltransferase subunits and multiple eraser proteins. As a m⁶A reader gene, SlYTP9 expression significantly affects the germination of tomato seeds, with promotion and inhibition in OE (overexpression) and RNAi (RNA interference) transgenic tomato plants, respectively. Microgravity promotes seed germination in the early germination period (0–3 days past germination; 0–3 DPG), but this promoting effect gradually disappears as the seedling grows (8–15 DPG). Further exploration revealed that this promoting effect is correlated with m⁶A modification, manifested as enhanced expression of most m⁶A writer genes; increased expression levels of overall reader genes; altered expression trends of some m⁶A eraser genes, particularly SlALKBH2; and enhanced m⁶A modification levels. The experimental results obtained in this study can provide a theoretical basis and evidence support for elucidating the role of m⁶A in tomato seed germination, as well as for exploring the interactions between seed germination, microgravity, and m⁶A modification. Full article

This study investigates the effects of astaxanthin on oxidative stress, mitochondrial function, and follicular development in mouse preantral follicles, with a focus on the involvement of the adenosine monophosphate-activated protein kinase (AMPK) signaling pathway. Astaxanthin (2.5 nM) significantly enhanced both the antrum formation (from 85.96% in the control group to 94.38% in the astaxanthin group) and maturation rates (from 79.15% to 85.12%) of oocytes (p < 0.05). From day 4 of in vitro culture, astaxanthin notably increased the area of follicle attachment (from 0.06 µm² to 0.32 µm²) and the secretion of estradiol (from 32.10 ng/L to 49.73 ng/L) (p < 0.05). Additionally, it significantly decreased malondialdehyde content (from 80.54 μM to 62.65 μM) within the follicles while increasing the mRNA expression levels of glutathione and superoxide dismutase 1 (p < 0.05). Astaxanthin also reduced reactive oxygen species levels in oocytes (p < 0.05). Notably, astaxanthin enhanced the expression of p-AMPK and PGC-1α, which are key proteins for the AMPK pathway; NRF1 and TFAM, which are crucial for mitochondrial biogenesis; NRF2 and HO-1, which protect against oxidative stress; CO1, CO2, CO3, ATP6, ATP8, and TOM20, which are essential for electron transport chain activity and ATP synthesis; PINK1, Parkin, and LC3-II, which are involved in mitophagy; Bcl-2, which inhibits cell apoptosis; and StAR and P450scc, which promote estrogen synthesis (p < 0.05). Furthermore, astaxanthin improved mitochondrial membrane potential and decreased the expression of cleaved caspase 3, Bax, and P53, which promotes cell apoptosis (p < 0.05). However, these changes induced by astaxanthin were completely reversed by AMPK inhibitors, indicating the involvement of the AMPK pathway. Conclusively, astaxanthin enhances the in vitro development of follicles, alleviates oxidative stress in preantral follicles, and promotes mitochondrial function during in vitro culture, which may be mediated by the AMPK pathway. Full article

Solanum lycopersicum, a widely cultivated vegetable crop globally, faces soil cadmium (Cd) contamination issues due to Cd’s high mobility, posing potential threats to Solanum lycopersicum growth and human health. In light of this, this study selected three representative Solanum lycopersicum varieties: Micro Tom, Red Guanyin, and Taiwan Pink King, and designed a series of experiments to investigate their growth performance under Cd stress. Experimental treatments included the sole application of different concentrations of Vitekang soil conditioner (VT), as well as the individual and combined application of VT and arbuscular mycorrhizal fungi (AMF). By thoroughly analyzing agronomic traits, cellular membrane lipid peroxidation levels, the activities of antioxidant enzymes (Catalase (CAT), Superoxide Dismutase (SOD), and Peroxidase (POD)), and the expression levels of genes related to Cd transport and detoxification (SLNRAMP6 and SlHMA3), this study comprehensively evaluated the effectiveness of different treatments in mitigating Cd stress in the three Solanum lycopersicum varieties. The results indicated that when VT was applied at a concentration of 2.4 g/kg in combination with AMF, it significantly reduced the detrimental effects of Cd on Micro Tom, Red Guanyin, and Taiwan Pink King. The specific experimental outcomes were as follows: (i) significantly decreased Cd accumulation in Solanum lycopersicum roots and leaves; (ii) effectively mitigated cellular membrane lipid peroxidation; (iii) significantly increased antioxidant enzyme activities; and (iv) influenced expression patterns of genes related to Cd transport and detoxification. This study further confirms that, compared to the sole application of VT or AMF, the combined application of these two treatments serves as a more effective practical method, exhibiting significant advantages in alleviating soil Cd contamination, promoting Solanum lycopersicum growth, and improving agronomic traits. This study not only advances research progress on VT and AMF in Solanum lycopersicumes, providing a solid theoretical and experimental foundation for cultivating high-quality Solanum lycopersicumes, but also holds significant importance for improving and optimizing the “VIP+N” technology, achieving farmland soil protection, and enhancing agricultural product quality. Full article

The presence of sufficient bone volumes is one of the most important criteria for the success of oral implant osseointegration. Therefore, the rehabilitation of edentulous atrophic maxillae represents the greatest challenge in terms of oral rehabilitation. Techniques such as bone grafts, angled implants, short implants, tuberosity, and pterygoid implants may not always be a viable alternative in the subsequent rehabilitation of the posterior atrophic maxilla. A breakthrough occurred when Brånemark first introduced longer, custom-designed implants inserted into the zygomatic bone to support craniofacial prosthesis in the 1980s. When used in the treatment of atrophic jaws, zygomatic implants provide a safe and effective alternative, with stable long-term results. Objectives: We aimed to retrospectively evaluate zygomatic bone resorption ten years after the placement of zygomatic implants. Methods: A retrospective observational study was designed to evaluate bone resorption over ten years following the placement of zygomatic implants. In a study group of 50 patients, using Hounsfield scales, the area of the zygoma and its bone density were established and evaluated. The NewTom NNT Analysis software (NewTom^®, Imola, Italy) was employed to trace the bone and implant limits on CBCT scans. Using this software, the three-dimensional information of the postoperative CBCT image was compared with the ten-year postoperative CBCT image, allowing for the assessment of the zygomatic bone resorption and bone density. Results: Highly significant statistical differences to an alpha level of 0.01 were identified between T0 (pre-op), T1 (12 months), and T2 (120 months) concerning zygomatic bone density, both in the first and in the second quadrants. The post hoc Bonferroni test revealed that significant statistical differences were observed between T0 and the remaining timepoints (T1 and T2), with the latter two exhibiting similar values. Conclusions: The evaluation of the resorption at the level of the zygoma, ten years after the placement of zygomatic implants, reveals that there are no significant losses between the initial and final controls. Therefore, it follows that this type of implant rehabilitation represents a viable alternative approach in patients with bone atrophy of the maxilla, offering a predictable therapeutic solution that enables immediate full function and excellent long-term success rates. However, we must not neglect the potential for future innovations in GBR involving the use of barrier membranes, either resorbable or non-resorbable, and even the application of titanium alveolar customized osteogenic scaffold, in conjunction with autologous bone grafts or biomaterials. Full article

Osteoarthritis (OA) is the most common rheumatologic disease and a major cause of pain and disability in older adults. No efficient treatment is currently available. Mitochondrial dysfunction in chondrocytes drives molecular dysregulation in OA pathogenesis. Recently, mitochondrial transfer to chondrocytes had been described, enabling transplant of mitochondria as a new avenue to modify the OA process, although evidence on its feasibility and safety remains limited.The primary objective of this study was to demonstrate the feasibility and safety of intra-articular mitochondrial transplantation. Mitochondria were isolated from liver using the procedure described by Preble and coworkers combined with magnetic beads coupled to anti-TOM22 antibodies. The organelles obtained were analyzed to determine their purity and viability. The safety and viability of the administration of the isolated mitochondria into articular tissues as well as the integration and distribution of the transplanted mitochondria within joint tissues were analyzed using both in vitro and in vivo models. We established an efficient, reproducible, effective, and rapid protocol for isolating mitochondria from liver. We obtained mitochondria with high viability, yield, and purity. The isolated mitochondria were injected into joint tissue using both in vitro and in vivo models. Functional mitochondria were detected in the extracellular matrix of the cartilage, menisci and synovium. Our results establish a safe and viable protocol for mitochondrial isolation and intra-articular injection. The methodology and findings presented here pave the way for future studies in osteoarthritis models to validate mitochondrial transplantation as a potentially effective treatment for OA. Full article

Background/Objectives: The study examined the relationships between specific Theory-of-Mind (ToM) dimensions, cognitive planning, and sleep duration in aging adults. Methods: The sample included 179 participants, comprising 46 cognitively healthy individuals, 75 diagnosed with amnestic Mild Cognitive Impairment (aMCI), and 58 with non-amnestic (naMCI). The mean age of the participants was 70.23 years (SD = 4.74), with a mean educational attainment of 12.35 years (SD = 3.22) and gender distribution of 53 men and 126 women. ToM assessment included tasks measuring the understanding and interpretation of non-literal speech, proverbs and metaphors, as well as an emotion-recognition test. For cognitive planning, a Tower Test was utilized. Sleep duration was measured using actigraphy. Results: We identified significant differences in various ToM tasks’ performance between the groups, particularly in non-literal speech tasks and third-order ToM stories. The HC group consistently outperformed both MCI groups in these tasks, with aMCI showing higher performance than naMCI. Mediation analysis applied to examine potential direct and indirect effects of sleep duration on ToM tasks indicated that total sleep time had significant indirect effects through cognitive planning—mainly as rule violation total score—on specific ToM aspects. Hence, besides the effects of MCI pathologies and especially of naMCI, sleep duration seems also to be associated with ToM performance in aging via specific executive functioning decrements. Conclusions: The findings underscore the social implications of ToM deficits due to MCI and/or sleep duration decrease, particularly in naMCI older adults, as they can seriously impair their social interactions. Targeted interventions could improve emotional understanding, communication, and overall quality of life. Full article

Background: Patellofemoral pain (PFP) significantly affects patients’ daily activities and consequently reduces their quality of life. Custom-made foot orthoses (FOs) are a common method of medical treatment that positively influences biomechanical factors such as the kinematics of the lower extremity and reduces pain perception in patients. However, there is a gap in research regarding the influence of different FO treatments on knee pain. Therefore, this study addresses the impact of biomechanical foot orthoses (BMFOs) and sensorimotor foot orthoses (SMFOs) on patients with foot deformity and PFP. Methods: A total of 26 participants (9 men, 17 women; 27.7 ± 10.7 years; 175.0 ± 0.1 cm; 75.7 ± 18.8 kg; BMI: 24.7 ± 5.6) took part in this randomized controlled clinical trial. In the pre-test, knee pain was evaluated using the Kujala Anterior Knee Pain Scale after the physician’s anamnesis and plantar pressure measurement. A 3-month intervention with SMFO and BMFO was performed, and weekly development was evaluated using 11-item visual analog scales (VASs). Repeated measures analyses of variance were used to assess differences between time of measurements (ToMs) and the interaction effect between ToMs and treatment groups (SMFO, BMFO). Results: Statistical analysis revealed no statistically significant interaction between ToMs and treatment groups but a significant main effect on Kujala anterior knee pain scores (M_Diff = 10.189; p = 0.014) and 12-week VAS (p = 0.001). Conclusions: The findings indicate that both treatment approaches effectively alleviated perceived knee pain in the PFP sample with foot deformity, with neither approach demonstrating superior efficacy. This trial was registered in the WHO International Clinical Trials Registry Platform (ICTRP) and German Clinical Trials Register (DRKS00035082). Full article

The efficiency of cloud seeding in enhancing precipitation is a subject of active debate within the scientific community. This work examines the impacts of cloud seeding in changing cloud properties and dynamics over Tom Green County in West Texas, USA, from 2015 to 2020. Several cloud categories including small, large, and type B are considered. The effect of cloud-seeding missions in changing clouds’ lifetime, area, volume, and precipitation mass is investigated. The results show that the average increase in the lifetime of small, large, and type B is 53.6, 27, and 3.5%, respectively, while the average area increased by 47.1, 27.5, and 5.0% respectively, and their average volume increased by 63.6, 33, and 5.6% respectively. A significant increase in the precipitation mass of the small, large, and type B clouds is observed after the seeding missions. From 2015 to 2020, the precipitation rates in seeded clouds are higher than the unseeded clouds. Comparing precipitation rates during the 2015–2020 cloud-seeding campaigns to the period from 2010 to 2014 before the campaigns shows no trend of increasing precipitation except during 2015 and 2016. Full article

Background and Aims: Cancer cachexia is a complex syndrome affecting most cancer patients and is directly responsible for about 20% of cancer-related deaths. Previous studies showed muscle proteolysis hyper-activation and mitophagy induction in tumor-bearing animals. While basal mitophagy is required for maintaining muscle mass and quality, excessive mitophagy promotes uncontrolled protein degradation, muscle loss and impaired function. BNIP3, a key mitophagy-related protein, is significantly increased in the muscles of both mice and human cancer hosts. This study aimed to define the potential of mitigating mitophagy via BNIP3 downregulation in preserving mitochondrial integrity, counteracting skeletal muscle loss in experimental cancer cachexia. Methods: Two in vivo gene delivery methods were performed to knock down muscle BNIP3: electroporation of a BNIP3-specific shRNA expression vector or adenovirus injection. Results: The electroporation effectively reduced muscle BNIP3 in healthy mice but was ineffective in C26 tumor-bearing mice. In contrast, adenovirus-mediated BNIP3 knockdown successfully decreased BNIP3 levels also in tumor hosts. Although BNIP3 knockdown did not impact overall on body or muscle mass, it improved muscle fiber size in C26-bearing miceh2, suggesting partial prevention of muscle atrophy. Mitochondrial respiratory chain complexes (OxPhos) and TOM20 protein levels were consistently rescued, indicating improvements in mitochondrial mass, while H₂O₂ levels were unchanged among the groups, suggesting that BNIP3 downregulation does not impair the endogenous control of oxidative balance. Conclusions: These findings suggest that a fine balance between mitochondrial disposal and biogenesis is fundamental for preserving muscle homeostasis and highlight a potential role for BNIP3 modulation against cancer-induced muscle wasting. Full article

Glaucoma is a heterogenous group of optic neuropathies characterized by the degeneration of optic nerve axons and the progressive loss of retinal ganglion cells (RGCs), which could ultimately lead to vision loss. Elevated intraocular pressure (IOP) is a major risk factor in the development of glaucoma, and reducing IOP remains the main therapeutic strategy. Endothelin-1 (ET-1), a potent vasoactive peptide, has been shown to produce neurodegenerative effects in animal models of glaucoma. However, the detailed mechanisms underlying ET-1-mediated neurodegeneration in glaucoma are not completely understood. In the current study, using a Seahorse Mitostress assay, we report that ET-1 treatment for 4 h and 24 h time points causes a significant decline in various parameters of mitochondrial function, including ATP production, maximal respiration, and spare respiratory capacity in cultured RGCs. This compromise in mitochondrial function could trigger activation of mitophagy as a quality control mechanism to restore RGC health. Contrary to our expectation, we observed a decrease in mitophagy following ET-1 treatment for 24 h in cultured RGCs. Using Morrison’s model of ocular hypertension in rats, we investigated here, for the first time, changes in mitophagosome formation by analyzing the co-localization of LC-3B and TOM20 in RGCs. We also injected ET-1 (24 h) into transgenic GFP-LC3 mice to analyze the formation of mitophagosomes in vivo. In Morrison’s model of ocular hypertension, as well as in ET-1 injected GFP-LC3 mice, we found a decrease in co-localization of LC3 and TOM20, indicating reduced mitophagy. Taken together, these results demonstrate that both ocular hypertension and ET-1 administration in rats and mice lead to reduced mitophagy, thus predisposing RGCs to neurodegeneration. Full article