Search Results (696)

Limnospira platensis is a promising sustainable biomass for functional food production. During cultivation, it secretes extracellular polysaccharides (EPS) with underutilized potential as food ingredients. This study aimed to strategically fractionate Spirulina EPS (SEPS) by molecular weight (MW: <30, 30–100, >100 kDa) to elucidate their structure-function relationships for targeted food applications. We found distinct functional diversification: The mid-MW fraction (SEPS-2, 30–100 kDa) was an amphiphilic glycoprotein complex with potential interfacial activity. The high-MW fraction (SEPS-3, >100 kDa) formed a dense, glucose-rich glucan network, suggesting utility as a natural thickener or texturizer. In contrast, the low-MW fraction (SEPS-1, <30 kDa), rich in deoxy-sugars, exhibited superior antioxidant capacity, indicating potential as a bioactive preservative or nutraceutical. Spectroscopic and morphological analyses linked these structural differences to their physicochemical properties. Notably, the 30–100 kDa fraction transitions from a cultivation byproduct to a functional food architect, where its interfacial properties can be leveraged to engineer stable, clean-label emulsion-based food products. This work provides a foundation for the valorization of L. platensis EPS, demonstrating how MW-directed fractionation can unlock tailored functionalities-from bioactive agents to structural polymers-for the development of next-generation foods from circular bioeconomy streams. Full article

Freeze–thaw procedures impair sperm morphology and function, affecting viability, motility, redox balance, and subcellular organization. Although antioxidants may mitigate these effects, their interaction with donor-specific variability remains unclear. We combined quantitative holotomography with conventional physiological assessments within a multivariate framework based on principal component analysis (PCA) and nested cross-validated Linear Discriminant Analysis (LDA) to evaluate donor-specific responses to antioxidant-supplemented cryopreservation. Spermatozoa from ten stallions was analyzed before and after freezing under five conditions: fresh semen; frozen semen with INRA Freeze, frozen semen with HF-20, and HF-20 supplemented with matcha, spirulina, horseradish, or quercetin. For each condition, sperm kinetics, mitochondrial activity, oxidative stress, DNA integrity, and three-dimensional volumetric measurements of whole-cell and subcellular compartments derived from holotomography were integrated into a single dataset. LDA achieved 0.734 cross-validated accuracy for stallion classification, revealing strong donor-specific signatures. In contrast, classification by antioxidant treatment was near chance (0.248). Fresh semen was clearly distinct from all cryopreserved groups. Holotomography showed reduced whole-cell and post-acrosomal/midpiece volumes after freezing, while nuclear volume was unchanged. Antioxidant supplementation produced minor, inconsistent effects, with partial midpiece preservation in some donors but no global pattern. Overall, inter-stallion variability dominates post-thaw sperm phenotype. Antioxidant effects were detectable but modest, supporting individualized strategies to optimize equine semen cryopreservation protocols. Full article

So far, space exploration has attracted increasing scientific interest due to the growth of missions promoted by private investment, such as SpaceX, Boeing, Blue Origin, and the recent attention generated by astronomical phenomena such as 3I/ATLAS. However, access to space experimentation remains limited and expensive. For this reason, new approaches to simulate space conditions on Earth are being developed to broaden research opportunities bio-inspired by plant responses to phototropism and geotropism. In this context, Betta Aerospace has continued the development of a microgravity simulation system consisting of a 3-axis clinostat powered by a single motor, continuous external electrical supply, and, in this project, a continuous external liquid supply. The proposed pioneer system was designed as a flexible platform manufactured through reinforced 3D printing, with an approximate size of 30 cm, an estimated payload of 30 kg, and a 24 V supply. Its main goal is to study the effects of simulated microgravity on aquatic organisms while enabling longer observation times in a controlled freshwater environment. Candidate biological samples include Ulva lactuca, Pyropia, Spirulina/Arthrospira, and Chlorella. Preliminary motion tests confirmed continuous operation at 10 rpm. In addition, a simplified static finite element analysis under a 294 N load yielded a maximum von Mises stress of 5.45 × 10⁷ Pa and a maximum displacement of 1.73 mm. Full article

Chlorella and Arthrospira (spirulina) are aquatic microalgae of increasing nutraceutical interest due to their dense bioactive composition and multi-target biological activity. This narrative review provides a comparative, mechanistically integrated synthesis of molecular mechanisms and clinical evidence related to their supplementation. Current data indicate that both microalgae converge on three central regulatory axes: activation of Nrf2-dependent antioxidant responses, attenuation of NF-κB-driven inflammatory signaling, and modulation of AMP-activated protein kinase (AMPK)/protein kinase B (AKT)-mediated metabolic pathways. Spirulina demonstrates stronger mechanistic links to intracellular signaling and more consistent clinical evidence for improvements in lipid profile, insulin sensitivity, and systemic inflammation. In contrast, chlorella provides complementary effects, particularly in antioxidant capacity, blood pressure regulation, gut microbiota modulation, and detoxification-related contexts. Their bioactive components—including phycocyanin, carotenoids, polysaccharides, and peptides—act synergistically to influence mitochondrial function, immune homeostasis, and metabolic resilience. While clinical findings are generally consistent, heterogeneity in study design and product standardization limits definitive conclusions. Overall, chlorella and spirulina emerge as complementary multi-pathway biological response modulators with potential applications in preventive and integrative medicine. Full article

In this study, the effects of different feeds provided during the initial feeding period of Trichogaster lalius (dwarf gourami) larvae on larval survival were evaluated. Larvae reared under laboratory conditions were assigned to four dietary treatments (EY: boiled chicken egg yolk, RO: live rotifers, IN: infusoria, SP: live Spirulina) during days 3–5 post hatching, after which all groups were enriched with live Artemia on days 5–10. A total of 4800 larvae were distributed across 16 treatment groups. On day 10, larval abundance and total length (mm) were recorded, and survival percentages were subsequently calculated. The pooled survival rates at the end of the experiment were 95.50 ± 4.05 for EY, 95.50 ± 2.53 for RO, 58.58 ± 8.88 for IN, and 77.25 ± 10.23 for SP. Statistical analysis revealed no significant differences in survival between the EY and RO groups (p > 0.05). In contrast, significant differences were observed between the IN group and the SP group, as well as between IN and the remaining treatment groups (p < 0.05). Moreover, no significant differences in total length were detected among the 16 groups at the end of the experiment (p > 0.303). Overall, the findings suggest that, under the present experimental conditions, larval survival of T. lalius was higher in the egg yolk and rotifer feeding treatments than in the infusoria and live Spirulina groups. Full article

Spirulina, a nutrient-rich microalga, was incorporated into wheat flour at 0–4% (flour basis) to evaluate its effects on dough mixing, rheology, water distribution, microstructure, and protein aggregation. The water absorption value increased from 56.2% (control) to 58.8–60.4% with Spirulina addition; however, the change was not statistically significant (p > 0.05). At 1–3% addition, development time increased from 2.2 to 2.4–2.7 min and stability from 4.9 to 6.4 min, while 4% addition reduced stability and increased weakening. Rheological measurements showed that G′ and G″ decreased by 23–33%, while tan δ increased from 0.37 to 0.38–0.39, indicating reduced viscoelastic strength. The LF-NMR results showed that A₂₁ exhibited an increasing trend (p > 0.05), accompanied by a prolongation of T₂₂, indicating enhanced water mobility and reduced binding strength. Microscopy showed a more continuous gluten network at 1–3%, whereas discontinuities appeared at 4%. SDS-PAGE indicated increased >60 kDa aggregates at 3% but reduced intensity at 4%. Overall, 1–3% Spirulina, particularly 3%, was associated with improved mixing tolerance and more uniform structural characteristics. Full article

The valorization of agro-industrial waste streams represents a promising strategy for reducing production costs in microalgae biotechnology while promoting circular economy approaches. In this study, wastewater derived from fig jam processing was evaluated as an organic carbon source for mixotrophic cultivation of Limnospira (Spirulina) platensis. Cultures were grown under four conditions: a control medium and three concentrations of fig wastewater (FW) at 0.75%, 1.5%, and 3% (v v⁻¹). The wastewater used in this study originates specifically from the washing and cleaning stages of dried fig processing, representing an early processing stream characterized by relatively high soluble sugar content and low thermal or chemical alteration. Biomass biochemical composition and bioactive compound production were investigated, including carbohydrates, proteins, lipids, photosynthetic pigments, polyphenols, antioxidant activity, and phycocyanin extraction yield and purity. The results showed that fig wastewater supplementation significantly influenced the metabolic profile of L. platensis. The highest protein content was obtained at 0.75% FW (44.90 ± 1.93 g 100 g⁻¹ DW), whereas lipid accumulation increased with FW concentration, reaching 9.45 ± 2.30 g 100 g⁻¹ DW at 3% FW. Antioxidant activity peaked at 1.5% FW (4.33 ± 0.43 μmol Trolox mg⁻¹ DW), suggesting stimulation of oxidative stress response pathways under moderate organic supplementation. Pigment production showed different responses, with relatively stable chlorophyll and carotenoid contents but decreasing phycocyanin levels at higher FW concentrations. Phycocyanin yield decreased from 9.82 ± 1.00 g 100 g⁻¹ DW in the control to 5.80 ± 0.22 g 100 g⁻¹ DW at 3% FW, while purity values were highest at the highest FW concentration. These findings demonstrate that fig processing wastewater can be effectively used as an alternative organic substrate for mixotrophic Spirulina cultivation, enabling simultaneous wastewater valorization and production of biomass rich in proteins and bioactive compounds. Full article

This research aimed to develop healthy and sustainable sea bream pâté products by using fish by-products. To this end, fishmeal was obtained from sea bream (Sparus aurata) by-products. Different formulations were developed: C (control pâté), B (pâté in which fish meat had been partially replaced by fishmeal), B-P (pâté containing fishmeal and pea protein), B-P-S (pâté containing fishmeal, pea protein and seaweeds), and B-Sp (pâté containing fishmeal and spirulina). The samples were subjected to physicochemical and microbiological characterization, as well as sensory evaluation. In addition, the secondary shelf life of the C and B pâtés was assessed. The results showed that including by-product meal in the formulations increased the protein content of the pâtés, as did pea protein and spirulina. Sensory analysis revealed that pâtés made with fish by-products were well accepted. The products containing algae received the lowest rating, although these products could be targeted at regular seaweed consumers. The incorporation of fishmeal made from sea bream by-products into fish pâtés, along with other ingredients, allowed a reduction in the proportion of fish in the formulation, thus obtaining innovative, healthy, and sustainable products. Full article

The rapid proliferation of microalgae in aquatic systems poses significant environmental and public health challenges, particularly in regions lacking adequate water treatment facilities. This study reports a sustainable approach for microalgae removal through the development of low-cost membranes derived from expanded polystyrene (EPS) waste and modified with polyethylene glycol (PEG) as a pore-forming agent. Membranes were fabricated via non-solvent-induced phase separation with PEG loadings of 0–20 wt.% and characterized in terms of morphology, porosity, wettability, and hydraulic performance. Filtration efficiency was evaluated using Spirulina platensis as a model microalga. Incorporation of PEG (up to 15 wt.%) enhanced membrane porosity (77–84%), improved hydrophilicity (water contact angle reduced from 68° to 48°), and increased water flux (10.98–39.2 L·m⁻²·h⁻¹), while maintaining complete microalgal rejection (100%). Optimized membranes exhibited asymmetric finger-like structures, contributing to improved permeability. Full article

Objectives: This study evaluates the research landscape of the cyanobacterium Spirulina (recently reclassified as Limnospira), a strategic resource in the nutraceutical, pharmaceutical, and functional food industries. The central objective is to transition from the traditional “superfood” narrative to a structured analysis of its modern therapeutic potential as reflected in current scientific literature. This study employs bibliometric analysis to highlight research trends and thematic directions in Spirulina-related studies, rather than to experimentally validate therapeutic effects. Methods: The investigation employed an exploratory bibliometric analysis of 996 peer-reviewed articles indexed in the Web of Science (2016–2025). Using VOSviewer software, we mapped keyword co-occurrence networks, international collaborations, and institutional clusters to identify dominant thematic directions and emerging research frontiers in biotechnology and medicine. Results: Bibliometric mapping illustrates research trends and thematic associations reported in the scientific literature centered on pathophysiological mechanisms, particularly oxidative stress, inflammation, and hepatoprotection. While often referred to as “microalgae”, Spirulina is biologically a photosynthetic prokaryote with a unique lipid profile characterized by high gamma-linolenic acid (GLA) content, although clinical evidence remains heterogeneous. The analysis highlights a robust regional research hub in the Middle East and North Africa, led by Egypt and Saudi Arabia, in contrast to fragmented inter-continental collaboration. Conclusions: The steady upward trend in publications confirms expanding academic interest in Spirulina as a functional ingredient. However, this study underscores a persistent gap between in vitro bioactivity and standardized clinical validation. These findings provide a roadmap for future biotechnological developments, emphasizing the need for more rigorous, multi-center clinical trials to bridge the “superfood” perception with evidence-based therapeutic applications. Full article

Background/Objectives: The presented study aimed to understand the relationship between knowledge, attitudes, and practices, as well as consumers’ preferences for the consumption of Spirulina-enriched fruit and vegetable juices. Methods: A survey about the consumers’ attitudes towards consumption of algae in general and especially Spirulina was conducted to better understand the target groups and marketing strategies for this novel non-alcoholic beverage product. Knowledge–Attitude–Practice (KAP) model in combination with structural equation modelling (SEM) was applied to test the hypothesised relationships between the variables. Additionally, consumers’ preference test was done using a seven-point hedonic scale and ranking of the six juice samples: plain sour cherry juice (SC1), sour cherry juice with 0.8% (SC2) and 1.6% (SC3) of blue Spirulina powder; plain tomato juice (T1), tomato juice with 0.8% (T2) and 1.6% (T3) of blue Spirulina powder. Results: The SEM results showed that there is a limited direct impact of knowledge on social motivation, while personal behaviour strongly predicts social motivation. Namely, perceived nutritional value and health benefits were shown to be the main factors for consumers’ willingness to drink Spirulina-enriched juice. Conclusions: The result of the consumer preference evaluation exposed that the juices containing sour cherry and Spirulina achieved better sensory acceptance and ranking than those containing tomato, pointing out the importance of the product matrix for achieving consumer acceptance. Full article

This study examined the life characteristics, fecundity, and innate immune response of fairy shrimp (Branchinella thailandensis Sanoamuang, Saengphan and Murugan, 2002) cultivated on Chlorella vulgaris, biofloc, dried powder Spirulina sp., and a formulated feed. These feeds were selected to compare traditional live feed (Chlorella vulgaris), microbial-based feed (biofloc), and alternative dry feed in order to identify a sustainable feeding strategy for fairy shrimp culture. The lifespans of male and female fairy shrimp reared on the biofloc diet were the longest at 44 ± 6.00 and 44.33 ± 4.67 days, respectively. Fairy shrimp reared on the biofloc diet demonstrated significantly enhanced growth, with lengths of 25.66 ± 1.75 mm in males and 27.60 ± 2.08 mm in females. The highest fecundity was also observed in the biofloc treatment, with 25 ± 6.65 broods per female, an average of 229.57 ± 2.08 eggs per brood, and a total of 5726.33 ± 1518.11 eggs per female over their lifespan. Fairy shrimp fed with Chlorella vulgaris, biofloc, and Spirulina sp. showed significantly high levels of superoxide dismutase (SOD) and lysozyme activities compared to those fed on a formulated feed, while malondialdehyde (MDA) levels, a marker of oxidative stress, were lower in these three groups. Results suggested that biofloc was a viable feed option for B. thailandensis, which positively influenced growth, lifespan, and fecundity while enhancing the innate immune function. Full article

Objective: Seaweed and microalgae provide antioxidants, polyunsaturated fatty acids, and bioactive compounds that may enhance exercise performance and accelerate recovery. However, evidence remains inconsistent. This systematic review and meta-analysis aimed to evaluate the effects of algae-derived supplementation on exercise performance and physiological recovery outcomes in healthy and athletic adults. Methods: This review was registered in PROSPERO (CRD420251166723) and conducted in accordance with PRISMA 2020 guidelines. PubMed, Web of Science, Embase, Cochrane Library, EBSCO, and CNKI were systematically searched for randomized controlled trials (RCTs) evaluating algae supplementation in exercise contexts. Inclusion and exclusion criteria were defined based on the PICOS framework. Primary outcomes included VO₂max, Time to exhaustion (TTE), maximal power output (WRmax), Time-Trial (TT) performance, and creatine kinase (CK). Standardized mean differences (SMDs) with 95% confidence intervals (CIs) were calculated using a random-effects model. Subgroup, sensitivity, and publication bias analyses were performed. Results: Twenty-two RCTs (n = 822) investigating Spirulina, Chlorella, brown-algal polysaccharides, or astaxanthin met inclusion criteria. Algae supplementation showed a suggestive improvement in VO₂max (SMD = 0.88, 95% CI: 0.00–1.75) and significantly improved in TTE (SMD = 1.06, 95% CI: 0.16–1.96), with smaller effects on WRmax (SMD = 0.29, 95% CI: 0.03–0.55), and no significant benefit for TT performance (SMD = −0.27, 95% CI: −0.74 to 0.21). Regarding recovery, CK concentrations were significantly reduced (SMD = −0.78, 95% CI: −1.28 to −0.28). Subgroup analysis suggested greater effects for Chlorella supplementation, higher dosages, and aerobic training contexts; reductions in muscle-damage markers were more evident following resistance exercise. Sensitivity analyses supported the robustness of the main findings with minimal evidence of publication bias. Conclusions: Algae-derived supplements—particularly Spirulina and Chlorella—may modestly enhance aerobic exercise performance and attenuate exercise-induced muscle damage under certain conditions. Effects appear to depend on algae species, dosing strategies, intervention duration, and training modality. High-quality, multi-center RCTs incorporating mechanistic endpoints are needed to clarify optimal application and to develop athlete-specific recommendations. Full article

Protein and phycocyanin production is challenged by freshwater scarcity in arid coastal regions. This study assessed and optimized the cultivation of Limnospira platensis BEA 1257B in full seawater. Eight cultivation phases were conducted in 10,000 L raceways under a greenhouse to evaluate the effects of seawater content, nutrient availability, shading, CO₂ supply, and medium recycling on biomass productivity and biochemical composition. Freshwater, energy, and fertilizer savings, together with effluent characteristics of the optimized full-seawater recirculation strategy (SWR), were evaluated against a conventional freshwater cultivation process. Lower productivity was associated with high salinity and irradiance. Under long-term optimized conditions (615 days), the strain achieved stable productivities of 4.1 ± 1.4 gDW m⁻² day⁻¹ (14.8 ± 5.0 tDW ha⁻¹ year⁻¹). Increasing salinity promoted carbohydrate accumulation in the biomass (26.0% AFWD), while protein (64.4%) and C-phycocyanin (9.9%) moderately decreased. Nevertheless, protein quality, phycocyanin, and essential fatty acids remained high. Spray-dried biomass exhibited nutritionally relevant contents of K, Mg, Ca, Fe, and Mn, and complied with international food safety standards. SWR reduced energy demand by 10.5% and freshwater consumption by 12% on a surface basis, although these advantages were partially offset when expressed per unit of product, while clearly supporting environmentally sustainable and regulatory-compliant Limnospira production. Full article

Marine-derived natural products are increasingly recognized for their therapeutic potential in cancer and other chronic diseases. Despite significant advances, current cancer treatments remain challenged by toxicity, drug resistance, and limited survival benefits. Natural compounds offer promising alternatives due to their multi-target mechanisms and favorable safety profiles. Among them, Spirulina, a filamentous cyanobacterium, stands out for its rich composition and diverse biological activities. Its anticancer effects involve apoptosis induction via intrinsic and extrinsic pathways, cell cycle arrest at G1/S or G2/M phases, inhibition of angiogenesis through the VEGF/VEGFR2 axis, and suppression of epithelial–mesenchymal transition. These activities are mainly attributed to C-phycocyanin, allophycocyanin, phenolic compounds, and immunomodulatory polysaccharides. Spirulina also exhibits potent immunomodulatory effects by enhancing natural killer cell activity, promoting M1 macrophage polarization, and regulating Th1 and Th17 cytokine responses, highlighting its potential as both an immunotherapeutic and chemoprotective agent. Moreover, preclinical findings suggest it may reduce chemotherapy-associated side effects. However, translation into clinical therapy remains limited by low bioavailability, lack of standardized extracts, and scarce clinical evidence. This review summarizes current mechanistic and immunological insights and highlights the need for optimized formulations, defined dosing strategies, and well-designed clinical trials to validate Spirulina’s potential in cancer treatment. Full article