Search Results (96)

Lacticaseibacillus rhamnosus J3205 was isolated from traditional fermented sauces and demonstrated potential probiotic properties. The strain exhibited high tolerance to simulated saliva (93.24% survival) and gastrointestinal conditions (69.95% gastric and 50.44% intestinal survival), along with strong adhesion capacity (58.25%) to intestinal epithelial cells. Safety assessments confirmed the absence of virulence and antibiotic resistance genes. Genomic analysis revealed stress-response genes and 34 insertion sequence (IS) elements, while proteomic profiling identified Pgk as a key enzyme in lactic acid production and SecY in oxidative stress resistance. Functionally, J3205 significantly reduces pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) and enhances antioxidant markers (SOD, GSH) in vitro. These results position L. rhamnosus J3205 as a promising candidate for gut-health foods, anti-inflammatory nutraceuticals, and oxidative-stress therapeutics, warranting further in vivo validation. Full article

This review article is devoted to the use of selenium nanoparticles (Se NPs) in plant production. The review analyzes relevant literature data for the last 10 years, considering the effect of Se NPs application on morphometric and biochemical parameters of plants. A number of actual works demonstrating the efficiency of Se NPs use in the composition of nanocomposites based on synthetic and natural polymers are considered separately. Possible mechanisms of Se NPs absorption and transport and their further activity in plant cells of agricultural crops in the context of biostimulating, biofortification, nutraceutical, and antioxidant activities of Se NPs, as well as the efficiency of Se NPs application under stress factors are discussed. The review provides data demonstrating the antibacterial and antifungal activities of Se NPs in the context of their activity against a wide range of phytopathogens. Also, we conduct a detailed comparative analysis of the relative efficiency of Se NP application with mineral Se-containing compounds (SeO₃²⁻ and SeO₄²⁻), as well as organic forms of Se (SeCys and SeMet). Full article

Background: Southern Brazil faced massive rains and floods in May 2024, which led to social, infrastructural, and One Health issues affecting over 478 municipalities and 2.3 million people. Exposure to floodwater increased the risk of bacterial infections, including leptospirosis. Despite the zoonotic nature of leptospiral infections, only human leptospirosis is subject to mandatory reporting, while canine cases are less closely monitored. Considering the extent of this climatic event, many emergency shelters were created for rescued dogs, highlighting the need to monitor infectious diseases to mitigate the spread of hazardous pathogens. Methods: We performed a molecular assessment of canine leptospirosis in Porto Alegre and its metropolitan region. A total of 246 dogs rescued from the flooded areas underwent molecular diagnosis targeting lipL32. In addition, positive samples were identified by sequencing of the partial secY gene. Results: A total of 9 (4%) dogs were positive for Leptospira spp. Molecular and phylogenetic analyses of secY from the positive samples determined that the circulating strains belonged to L. interrogans (n = 8)—Icterohaemorrhagiae and Pomona as the suggested serogroups—and L. kirschneri (n = 1). Conclusions: Our findings point out the challenges in diagnosing and controlling leptospirosis during severe climatic events and reinforce the need for preventive sanitary measures to mitigate the dissemination of Leptospira spp., including the adoption of a mandatory notification system for canine leptospirosis. Full article

Background: The integration of Artificial Intelligence (AI) into Business Process Management Systems (BPMSs) has led to the emergence of AI-Augmented Business Process Management Systems (ABPMSs). These systems offer dynamic adaptation, real-time process optimization, and enhanced knowledge management capabilities. However, key challenges remain, particularly regarding explainability, user engagement, and behavioral integration. Methods: This study presents a novel framework that synergistically integrates the Socialization, Externalization, Combination, and Internalization knowledge model (SECI), Agile methods (specifically Scrum), and cutting-edge AI technologies, including explainable AI (XAI), process mining, and Robotic Process Automation (RPA). The framework enables the formalization, verification, and sharing of knowledge via a well-organized, user-friendly software platform and collaborative practices, especially Communities of Practice (CoPs). Results: The framework emphasizes situation-aware explainability, modular adoption, and continuous improvement to ensure effective human–AI collaboration. It provides theoretical and practical mechanisms for aligning AI capabilities with organizational knowledge management. Conclusions: The proposed framework facilitates the transition from traditional BPMSs to more sophisticated ABPMSs by leveraging structured methodologies and technologies. The approach enhances knowledge exchange and process evolution, supported by detailed modeling using BPMN 2.0. Full article

Selenium (Se) is an essential trace element for humans, and the production of Se-enriched rice (Oryza sativa) is a key approach for Se supplementation. Nevertheless, the effects of different Se forms and concentrations on the metabolism and aboveground absorption pathways of rice seedlings are not yet well-understood. Therefore, we conducted a hydroponic experiment and used transcriptome analysis to study the absorption and transformation processes of sodium selenite (Na₂SeO₃) and selenomethionine (SeMet) in rice at the seedling stage. The aboveground (stem + leaf) Se concentration at the seedling stage was higher under the SeMet treatments, and low Se applications (<25 μM) significantly promoted rice growth. Selenocysteine (SeCys) and SeMet were the primary forms of Se in rice, accounting for 57–86.35% and 7.6–31.5%, respectively, while selenate [Se (VI)] significantly increased when Se levels exceeded 25 μM. In the transcriptome, differentially expressed genes (DEGs) were significantly enriched in the following pathways: carbon metabolism, amino acid biosynthesis, and glutathione metabolism. In the Na₂SeO₃ treatments, genes encoding phosphoglycerate mutase (PGM), triosephosphate isomerase (TPI), phosphofructokinase (PFK), pyruvate kinase (PK), malate dehydrogenase (MDH), polyamine oxidase (PAO), aspartate aminotransferase (AST), and glutathione S-transferase (GST) were upregulated, and the expression levels of differentially expressed genes (DEGs) decreased with increasing Se levels. SeMet treatments upregulated genes encoding PFK, PK, glutamine synthetase (NADH-GOGAT), and L-ascorbate peroxidase (APX), and expression levels of DEGs increased with increasing Se levels. This study provides important insights into the molecular mechanisms of the uptake and metabolism of different Se forms in rice at the seedling stage. Full article

Leptospirosis is a zoonotic disease of global public health importance caused by bacteria of the genus Leptospira. Small non-flying mammals are important reservoirs of the pathogen. The Brazilian Atlantic Forest is a biodiversity hotspot located in a densely populated area and subject to intense degradation. Although documented through serosurveys and the detection of leptospiral DNA in wild small mammals, no study has performed a genetic characterization of the bacteria in the region. The present study aimed to evaluate the genetic diversity of pathogenic leptospires identified in small non-flying mammals in the Southeast Atlantic Forest and to perform intraspecific genetic inferences with other hosts. The studied area included five different conservation units. Molecular diagnosis was performed based on the lipl32 gene. The SLST typing method was applied based on the secY gene. In total, 56% of samples were lipL32-PCR-positive and identified as L. interrogans, with a high genetic identity among them, distributed in four main haplogroups. The largest haplogroup also included reference sequences from humans, dogs, and urban rats, all belonging to the Icterohaemorrhagiae serogroup. Our results reinforce the role of small mammals as important carriers of L. interrogans and highlight the Atlantic Forest as a significant environment for the circulation and dissemination of spirochetes with zoonotic potential. Full article

Selenium (Se) is an essential micronutrient, yet its deficiency remains a global concern. This study investigates the biofortification of alfalfa (Medicago sativa cv. ProINTA Super Monarca GR9) via foliar Se application to enhance Se accumulation and transformation into bioavailable organic forms. A controlled environment experiment in a plant growth chamber and a one-season open-field trial (January 2023, Argentina) were conducted. Treatments included sodium selenate (Se(VI)), sodium selenite (Se(IV)), and a 1:1 mixture, applied at 45 and 90 g Se ha⁻¹, with and without the biostimulant BIOFORGE^®. Treated plants exhibited increased Se content, correlating with the applied doses. X-ray absorption spectroscopy (XAS) confirmed that most inorganic Se was transformed into organic Se forms, with Se(IV) treatments yielding the highest concentrations of organic Se species such as selenocysteine (SeCys) and selenomethionine (SeMet). Open-field trials showed a complete conversion of Se, though total Se accumulation was lower than in controlled conditions. Se treatments did not affect forage quality or biomass production. The biostimulant slightly reduced Se uptake but did not compromise biofortification. These results highlight Se(IV) as the optimal treatment for alfalfa biofortification, presenting a sustainable strategy to enhance dietary Se intake through functional foods. Full article

Selenium (Se) is an essential micronutrient, recognized for its role in cellular redox systems and its therapeutic potential in cancer treatment. Organic selenium compounds, particularly selenocystine (SeCys), have demonstrated anticancer efficacy due to the ability to induce apoptosis and enhance the effects of chemotherapy agents. Recent studies have shown that SeCys exhibits selective toxicity against cancer cells while sparing normal cells. Unfortunately, its clinical application is limited by stability and solubility concerns. A possible solution to overcome these hurdles comes from recent advances in functionalized nanomaterials. In this study, we investigate the possible incorporation of SeCys with hydroxyapatite nanoparticles (HASeCys) via various methods (adsorption, co-precipitation, and co-precipitation through thermal decomplexation), resulting in the formation of nanocomposites with elemental selenium. The highest elemental selenium yield was achieved with a thermal decomplexing co-precipitation, highlighting the influence of synthesis parameters on Se allotrope formation. Finally, as a preliminary investigation, the HASeCys samples were tested on a panel of cancer cell lines, showing an interesting activity when the hydroxyapatite nanocrystals were functionalized with both crystalline gray and amorphous red selenium. Full article

Bovine genital leptospirosis (BGL) is a chronic reproductive disease in cattle, often causing significant economic losses, and is commonly associated with leptospiral strains belonging to the Sejroe serogroup. A two-step protocol was recommended, based on serological screening of the herds followed by an individual diagnosis with PCR of a genital sample. Although proposed, it has not been commonly applied under field conditions, leading to frustrating outcomes in disease control. In that context, the present study aimed to demonstrate the viability of that two-step protocol under field conditions for diagnosing BGL in eight herds with reproductive disorders. Blood samples were collected from 440 cows for serology. In addition, 304 cervicovaginal mucus (CVM) samples were collected for lipL32-PCR and 11 samples were sequenced of the secY gene. All herds showed high seroreactivity, mainly against the serogroup Sejroe. In addition, 113 of the 304 CVM samples (37.2%) were PCR-positive. DNA sequencing of 11 positive samples based on the secY gene revealed maximum identity (100%) with L. interrogans species. This study found CVM sampling quick and easy, making it practical for field use. Overall, the results support the two-step protocol serological screening followed by CVM-PCR testing as an efficient and reliable method for diagnosing BGL in herds with reproductive disorders in field conditions. Full article

In this study, C. pyrenoidosa were cultured with seven different concentrations of Na₂SeO₄ (0–10 mg/L), and the effects of Na₂SeO₄ on the growth, Se-forms, and nutritional quality of C. pyrenoidosa were explored. The results showed that at the concentration of 0.5 mg/L Na₂SeO₄, the C. pyrenoidosa were plump and healthy; the contents of biomass, soluble protein, lipids, and TPUFA reached the highest level; the total Se content in C. pyrenoidosa increased with the increasing Na₂SeO₄ concentrations. However, the proportion of organic Se in C. pyrenoidosa. reached the highest value of 87.58% at the concentration of 0.5 mg/L Na₂SeO₄. Among organic Se forms, SeMet accounted for the largest proportion, while MeSeCys accounted for a relatively smaller proportion, but SeCys₂ was not detected. The addition of Na₂SeO₄ (except for ≤0.5 mg/L) reduced the contents of photosynthetic pigments in C. pyrenoidosa. In addition, the antioxidant capacity of C. pyrenoidosa first increased and then decreased with the increasing Na₂SeO₄ concentrations, but different enzymes exhibited different tolerances to Na₂SeO₄. Based on the above research results, 0.5 mg/L Na₂SeO₄ concentration is recommended for the production of Se-rich C. pyrenoidosa. Our findings will provide a theoretical basis and practical references for the development of Se-rich C. pyrenoidosa health care products. Full article

In the post-COVID-19 era, education requires teachers to engage learners across diverse learning environments (at school or other formal institutions, at home, outdoors, or in virtual environments) using innovative learning strategies. To meet these challenges, teachers must upskill their creativity and strengthen their pedagogical digital competencies and knowledge management skills. This study introduces the innovative teacher training and support (TTS-IPCD) model to enhance teachers’ creativity and pedagogical digital competencies. This research involved a sample of 350 teachers from 75 primary and secondary schools over a four-year period. Teachers’ creativity was measured using the Kirton Adaption–Innovation Inventory (KAI), assessing key metrics such as problem-solving flexibility, openness to change, and inclination toward novel approaches. Quantitative analysis was conducted using an independent samples t-test to evaluate teacher creativity changes. The results indicated that the TTS-IPCD model enhanced teacher creativity in the direction of a stronger propensity toward innovative behaviors, including embracing diversity and change in their work, solving problems through novel approaches, and adopting a holistic perspective rather than strictly adhering to established routines. Furthermore, the TTS-IPCD model improved teamwork and collaboration, contributing to the development of more adaptive and innovative learning environments. These findings highlight the importance of continuous professional development of teachers focused on creative pedagogy and digital competencies to equip teachers for the evolving educational landscape. Full article

Cardamine violifolia is a selenium (Se)-rich vegetable crop belonging to the Brassicaceae family. This study investigated the Se concentration and volatiles in the fresh (CK) C. violifolia, natural fermented (NF), Lactiplantibacillus plantarum (LP), and Leuconostoc mesenteroides (LM) fermented C. violifolia pickles. Results showed that fermentation promoted the levels of selenocysteine, methyl selenocysteine, and selenate. A total of 648 volatile compounds were found, including 119 terpenoids, 105 heterocyclic compounds, 103 esters, and 65 hydrocarbons. Differential analysis of volatiles indicated that fermentation induced the release of volatiles when compared to CK, whereas volatile profiles in LM and NF pickles showed notable differences from LP pickles. SeCys2, MeSeCys, and selenate significantly correlated to several volatile compounds, implying that Se metabolism may affect the formation of volatiles. Conclusively, fermentation promoted the release of aroma and bioactive volatiles and the degradation of unpleasant and harmful substances in C. violifolia pickles. Full article

This article proposes the application of the AHP method in an office furniture remanufacturing company, with the aim of optimizing knowledge retention and management. In particular, it seeks to establish the optimal retrieval route for returned products. To this end, a bibliographic analysis was first carried out, which revealed the scarcity of previous studies on the subject, thus validating the relevance of this work. Subsequently, a practical application of the AHP method was carried out to define the weighting matrix of the evaluation criteria, applied to three specific pieces of furniture, which confirmed the effectiveness of the tool. In a complementary manner, Nonaka and Takeuchi’s SECI model of knowledge management was used, guaranteeing the continuous updating of the matrices and the adequate retention of knowledge in the company. This methodology will increase the volume of remanufactured products and improve operating margins. By reaping both the economic and environmental benefits of this practice, the company will be able to reduce costs, generate additional revenue, improve its corporate image, and build customer loyalty. At the same time, this study promotes the sustainability and sustainable development of this practice within the company and, by extension, in the broader office furniture manufacturing industry. It can serve as a reference for other companies in this sector across different countries. Full article

The quality evaluation of processed potatoes is vital in the food industry. In this study, the effect of three different drying methods on the post-processing quality of potatoes utilizing 4, 8, 12, and 16 h of freeze drying (FD), infrared drying (ID), and oven drying (OD) was investigated. The impact of the drying methods on the potato’s microstructure was analyzed and quantified using 3D X-ray micro-computed tomography images. A new Hybrid Quality Score Evaluator (HQSE) was introduced and used to assess the Quality Index (QI) and Specific Energy Consumption Index (SECI) across various drying methods and durations. Mathematical models were developed to predict the optimal drying method. FD showed significantly higher (p < 0.05) colour retention, rehydration ratio, and total porosity, with minimal shrinkage, although it had higher energy consumption. ID had the shortest drying time, followed by OD and FD. The optimization showed that for FD, the optimal time of 5.78 h increased QI by 9.7% and SECI by 30.6%. The mathematical models could accurately predict the QI and SECI under different drying methods, balancing quality preservation with energy efficiency. The findings suggest that a hybrid drying system could optimize potato quality and energy consumption. Full article

Sequence analysis of the Zaire ebolavirus (EBOV) polymerase (L gene) mRNA, using online tools, identified a highly ranked −1 programmed ribosomal frameshift (FS) signal including an ideal slippery sequence heptamer (UUUAAAA), with an overlapping coding region featuring two tandem UGA codons, immediately followed by an RNA region that is the inverse complement (antisense) to a region of the mRNA of the selenoprotein iodothyronine deiodinase II (DIO2). This antisense interaction was confirmed in vitro via electrophoretic gel shift assay, using cDNAs at the EBOV and DIO2 segments. The formation of a duplex between the two mRNAs could trigger the ribosomal frameshift, by mimicking the enhancing role of a pseudoknot structure, while providing access to the selenocysteine insertion sequence (SECIS) element contained in the DIO2 mRNA. This process would allow the −1 frame UGA codons to be recoded as selenocysteine, forming part of a C-terminal module in a low abundance truncated isoform of the viral polymerase, potentially functioning in a redox role. Remarkably, 90 bases downstream of the −1 FS site, an active +1 FS site can be demonstrated, which, via a return to the zero frame, would enable the attachment of the entire C-terminal of the polymerase protein. Using a construct with upstream and downstream reporter genes, spanning a wildtype or mutated viral insert, we show significant +1 ribosomal frameshifting at this site. Acting singly or together, frameshifting at these sites (both of which are highly conserved in EBOV strains) could enable the expression of several modified isoforms of the polymerase. The 3D modeling of the predicted EBOV polymerase FS variants using the AI tool, AlphaFold, reveals a peroxiredoxin-like active site with arginine and threonine residues adjacent to a putative UGA-encoded selenocysteine, located on the back of the polymerase “hand”. This module could serve to protect the viral RNA from peroxidative damage. Full article