Search Results (15)

Habitat fragmentation poses a significant threat to the existence and reproduction of large carnivores, such as brown bears, as it affects the genetic connectivity of populations and, consequently, their long-term viability. Understanding the genetic makeup and dispersal patterns in areas where brown bears live is crucial for developing effective conservation plans and promoting human-brown bear coexistence. In this study, 214 hair samples were collected non-invasively from brown bears and were genetically analyzed using fifteen specific microsatellite loci to shed light on the genetic status and demography of a sub-population residing in Central Greece (Trikala-Meteora area). The broader Central and South Pindos regions have not been studied for over a decade; however, high levels of genetic diversity, a lack of heterozygosity deficiency, and no signs of inbreeding, along with an estimated effective population size (Ne = 99), confirm the good conservation status of this sub-population. Moreover, the existence of a natural and apparently functional corridor in Central Pindos was demonstrated by bear movements between the western and eastern parts of the project area, enhancing the sustainability of the population. These findings will contribute to future efforts to conserve natural corridors that enable functional connectivity among large areas of brown bear territories in Greece. Full article

West Nile virus (WNV), a zoonotic mosquito-borne virus, has recently caused human outbreaks in Europe, including Greece. Its transmission cycle in nature includes wild birds as amplifying hosts and ornithophilic mosquito vectors. The aim of this study was to assess WNV circulation among wild birds from two regions of Greece, Peloponnese and Western Greece, during 2022. To this end, a total of 511 birds belonging to 37 different species were sampled and molecularly screened. WNV RNA was detected from February to November in a total of 71 wild birds of nine species originating from both investigated regions. The first eight positive samples were sequenced on a part of NS3 and, according to the phylogenetic analysis, they belonged to evolutionary lineage 2 and presented similarity to previous outbreak-causing Greek strains (Argolis 2017, Macedonia 2010 and 2012). It was more likely to identify a PCR positive bird as the population density and the distance from water sources decreased. The present report provides evidence of WNV occurrence in both Peloponnese and Western Greece during 2022 and underlines its possible overwintering, highlighting the need for avian species surveillance to be conducted annually and throughout the year. Magpies are proposed as sentinels for WNV monitoring. Full article

Health equipment are used to keep track of significant health indicators, automate health interventions, and analyze health indicators. People have begun using mobile applications to track health characteristics and medical demands because devices are now linked to high-speed internet and mobile phones. Such a combination of smart devices, the internet, and mobile applications expands the usage of remote health monitoring through the Internet of Medical Things (IoMT). The accessibility and unpredictable aspects of IoMT create massive security and confidentiality threats in IoMT systems. In this paper, Octopus and Physically Unclonable Functions (PUFs) are used to provide privacy to the healthcare device by masking the data, and machine learning (ML) techniques are used to retrieve the health data back and reduce security breaches on networks. This technique has exhibited 99.45% accuracy, which proves that this technique could be used to secure health data with masking. Full article

Better alignment between health research organisations with the needs (and interests) of key stakeholders in the health policy and research system is critical to improving research impact. The George Institute for Global Health’s ‘Healthier Societies’ program focuses on harnessing the power of governments, markets, and communities to improve population level health equity outcomes and maximise research impact. This protocol outlines a systemic multi-sectoral approach to advance health research impact globally applied to a project to reduce population salt intake in Vietnam by introducing reduced-sodium salts and salty condiments. We defined a systemic multi-sectoral approach to be a strategy that involves engaging with government, market and communities in a deliberate and joined-up way to solve a problem in which they all have a role to play. The project objectives are to: (i) produce reduced-sodium fish sauce products and test consumer acceptability; (ii) investigate the market feasibility of introducing reduced-sodium foods (salt, bot canh and fish sauce) into the Vietnamese market; (iii) estimate the cost-effectiveness of three different government strategies to support the implementation of reduced-sodium products; and (iv) develop an advocacy roadmap to maximise potential research impact. Methods will include standard quality and safety assessments, consumer sensory testing for the locally produced reduced-sodium fish sauces, market feasibility assessment (including collating market data and semi-structured interviews with stakeholders), cost-effectiveness modelling (Markov cohort model), multi-sector stakeholder engagement, and the development of a coordinated advocacy strategy using the Kotter Plus framework. Health research organisations are increasingly seeking ways to achieve greater impact with their research. Through the application of a systemic multi-sectoral approach with governments, markets and communities, this protocol provides an example of how health research projects can achieve such impact. Full article

In order to optimize the appropriate conservation actions for the brown bear (Ursus arctos L.) population in Greece, we estimated the census (Nc) and effective (Ne) population size as well as the genetic status of brown bear sub-populations in three National Parks (NP): Prespa (MBPNP), Pindos (PINDNP), and Rhodopi (RMNP). The Prespa and Pindos sub-populations are located in western Greece and the Rhodopi population is located in eastern Greece. We extracted DNA from 472 hair samples and amplified through PCR 10 microsatellite loci. In total, 257 of 472 samples (54.5%) were genotyped for 6–10 microsatellite loci. Genetic analysis revealed that the Ne was 35, 118, and 61 individuals in MBPNP, PINDNP, and RMNP, respectively, while high levels of inbreeding were found in Prespa and Rhodopi but not in Pindos. Moreover, analysis of genetic structure showed that the Pindos population is genetically distinct, whereas Prespa and Rhodopi show mutual overlaps. Finally, we found a notable gene flow from Prespa to Rhodopi (10.19%) and from Rhodopi to Prespa (14.96%). Therefore, targeted actions for the conservation of the bears that live in the abovementioned areas must be undertaken, in order to ensure the species’ viability and to preserve the corridors that allow connectivity between the bear sub-populations in Greece. Full article

This paper aimed to define two critical mechanical properties of leather—Young’s modulus and Poisson ratio—essential to the virtual simulation of the behaviour of the footwear uppers against the manufacturing operations of stitching and perforating. The following technological aspects were considered to analyse the materials from manufacturing conditions point of view: the number of layers (one layer and two layers), the nature of the materials used for uppers subgroups (calfskin-outer upper, sheep leather-lining, polyester knitted fabric-lining), the overlapping width in the stitching area, the number of parallel stitches (single stitch and double stitch), the punching interval and the type of perforations (simple and with eyelets), resulting in nine kinds of samples. Furthermore, the elasticity (Young’s modulus) and lateral contraction (Poisson’s ratio) were calculated during the tensile strength analysis performed on the SATRA STM 466 equipment. Both mechanical parameters are essential to simulate the behaviour of the virtual footwear prototypes in various conditions. Full article

The renin-angiotensin system (RAS), besides being a major regulator of blood pressure, is also involved in tumor angiogenesis. Emerging evidence suggests a correlation between the use of pharmacologic RAS inhibitors and a delay in urothelial bladder cancer (BC) progression. However, it is unknown whether RAS gene variants may predispose to the development of BC. This study examined the association of RAS single nucleotide polymorphisms (SNPs) including AT1R rs5186, AT2R rs11091046, REN rs12750834, ANG rs4762, and ANG rs699 with the risk of developing non-invasive BC. Peripheral blood samples from 73 patients with T1 urothelial BC (66 men, seven women) and an equal number of healthy subjects (control group) were collected. The TT genotype of the REN rs12750834 SNP (OR: 2.8 [1.3–6.05], p = 0.008) and to a lesser extent the presence of the T allele (OR: 2.3 [1.2–4.48], p = 0.01) conferred a higher risk of BC. The highest risk for BC within SNP carriers of the RAS system was associated with the presence of the CC genotype (OR: 17.6 [7.5–41.35], p < 0.001) and C allele (OR: 17.7 [8.8–35.9], p < 0.001) of the ANG rs699 SNP. The presence of the AT2R rs11091046 SNP, particularly the AA genotype, was associated with a protective effect against developing BC (OR: 0.268 [0.126–057], p < 0.001). In conclusion, these results support the clinical utility of RAS gene SNPs AT2R rs11091046, REN rs12750834, and ANG rs699 in the genetic cancer risk assessment of patients and families with BC. Full article

We recently reported that the inability of osteoarthritic (OA) chondrocytes to repair oxidative stress (OS) induced DNA damage is linked to Cav-1 overexpression/improper localization. We speculated that the senescent status of OA cells was responsible for this Cav-1 dysregulation. Here, to further investigate this hypothesis, we used Wharton Jelly derived mesenchymal stem cells (WJ-MSCs) and investigated Cav-1 function as cells reached replicative senescence or upon stress induced senescence (SIPS). We showed that Cav-1 is upregulated, phosphorylated and translocated to the nucleus in young WJ-MSCs upon acute exogenous OS, and that it returns back to basal/nonphosphorylated levels and exports the nucleus in the recovery phase. However, as cells reach senescence, this regulation is lost. OS did not induce any Cav-1-mediated response, which is concomitant with the inability of older cells to restore DNA damage. Furthermore, downregulation of Cav-1 resulted in persistent OS-induced DNA damage and subsequent onset of senescence. We also report that the establishment of senescence is mediated by autophagy stimulation, since downregulation of autophagy key molecule Atg5, simultaneously with Cav-1 downregulation, was found to inhibit SIPS. Basically, we propose that Cav-1 involvement in DNA damage response can lead to senescence, either because the damage is extensive or because Cav-1 is absent/unable to perform its homeostatic role. Full article

The use of artificial intelligence (AI) is increasing in various sectors of photovoltaic (PV) systems, due to the increasing computational power, tools and data generation. The currently employed methods for various functions of the solar PV industry related to design, forecasting, control, and maintenance have been found to deliver relatively inaccurate results. Further, the use of AI to perform these tasks achieved a higher degree of accuracy and precision and is now a highly interesting topic. In this context, this paper aims to investigate how AI techniques impact the PV value chain. The investigation consists of mapping the currently available AI technologies, identifying possible future uses of AI, and also quantifying their advantages and disadvantages in regard to the conventional mechanisms. Full article

The aim of this study was to evaluate the diagnostic performance of a multiplex bead assay for the simultaneous detection of antibodies against Mycobacterium bovis, Brucella suis, and Trichinella spiralis. Sera from Eurasian wild boar of known serological status for TB (64 seropositive, 106 seronegative), Brucella (30 seropositive, 39 seronegative), and Trichinella (21 seropositive, 97 seronegative) were used for the development and evaluation of the assay. Magnetic beads coated with recombinant MPB83 antigen (TB), a whole-cell B. suis 1330 antigen, and an E/S T. spiralis antigen were used for the detection of specific antibodies using Bio-Rad Bio-Plex technology. The sensitivities (Se) and specificities (Sp) of the multiplex assay were, for M. bovis, 0.98 and 0.86; for B. suis, 1.00 and 0.97; and for T. spiralis, 0.90 and 0.99 (Se and Sp, respectively). The results show the diagnostic potential of this assay for the simultaneous detection of antibodies against M. bovis, B. suis, and T. spiralis in wild boar. Full article

Resistance mediated by β-lactamases is a globally spread menace. The aim of the present study was to determine the occurrence of Escherichia coli producing plasmid-encoded AmpC β-lactamases (pAmpC) in animals. Fecal samples from chickens (n = 159), cattle (n = 104), pigs (n = 214), and various wild bird species (n = 168), collected from different Greek regions during 2018–2020, were screened for the presence of pAmpC-encoding genes. Thirteen E. coli displaying resistance to third-generation cephalosporins and a positive AmpC confirmation test were detected. bla_CMY-2 was the sole pAmpC gene identified in 12 chickens’ and 1 wild bird (Eurasian magpie) isolates and was in all cases linked to an upstream ISEcp1-like element. The isolates were classified into five different sequence types: ST131, ST117, ST155, ST429, and ST1415. Four chickens’ stains were assigned to ST131, while five chickens’ strains and the one from the Eurasian magpie belonged to ST117. Seven pAmpC isolates co-harbored genes conferring resistance to tetracyclines (tetM, tetB, tetC, tetD), 3 carried sulfonamide resistance genes (sulI and sulII), and 10 displayed mutations in the quinolone resistance-determining regions of gyrA (S83L+D87N) and parC (S80I+E84V). This report provides evidence of pAmpC dissemination, describing for the first time the presence of CMY-2 in chickens and wild birds from Greece. Full article

The use of slip-resistant winter footwear is crucial for the prevention of slips and falls on ice and snow. The main objective of this paper is to evaluate a mechanical testing method to determine footwear slip resistance on wet and dry ice surfaces and to compare it with the human-centred test method introduced by researchers at KITE (Knowledge, Innovation, Talent, Everywhere)-Toronto Rehabilitation Institute-University Health Network. Phase 1 of this study assessed the repeatability and reproducibility of the mechanical method by evaluating ten different occupational winter boots using two SATRA Slip resistance testers (STM 603, SATRA Technology Centre, Kettering, UK). One tester is located in Toronto and one in Montreal. These boots were chosen based on the needs of the IRSST (Institut de Recherche Robert-Sauvé en Santé et en Sécurité du Travail, Montréal, Quebec, Canada), who were primarily interested in providing safe winter footwear for police, firefighters and municipal workers. In Phase 2, the results of the human-centred test approach were compared with the mechanical results. In Phase 3, two of these boots with conflicting results from the previous phases were tested using a second human-centred method. In Phase 1, the mechanical testing results obtained in the two labs showed a high linear correlation (>0.94) and good agreement on both ice surfaces; however, they revealed a bias (~0.06) between the two labs on the dry ice condition. The mechanical and human-centred tests (phase 2) were found to be better correlated in the wet ice condition (R = 0.95) compared to the dry ice condition (R = 0.34). Finally, the rating of the footwear slip resistance based on the number of slips counted in phase 3 was consistent with the rating by the human-centred test method (phase 2), but not the mechanical method (phase 1). The findings of this study provide a better understanding of the limitations of the SATRA ice tray for measuring footwear slip resistance and demonstrate that the mechanical method must be further refined to make it more comparable to the human-centred methods to achieve better agreement with real-world performance. Full article

A radial basis function neural network-based 2-satisfiability reverse analysis (RBFNN-2SATRA) primarily depends on adequately obtaining the linear optimal output weights, alongside the lowest iteration error. This study aims to investigate the effectiveness, as well as the capability of the artificial immune system (AIS) algorithm in RBFNN-2SATRA. Moreover, it aims to improve the output linearity to obtain the optimal output weights. In this paper, the artificial immune system (AIS) algorithm will be introduced and implemented to enhance the effectiveness of the connection weights throughout the RBFNN-2SATRA training. To prove that the introduced method functions efficiently, five well-established datasets were solved. Moreover, the use of AIS for the RBFNN-2SATRA training is compared with the genetic algorithm (GA), differential evolution (DE), particle swarm optimization (PSO), and artificial bee colony (ABC) algorithms. In terms of measurements and accuracy, the simulation results showed that the proposed method outperformed in the terms of Mean Absolute Error (MAE), Mean Absolute Percentage Error (MAPE), Root Mean Square Error (RMSE), Schwarz Bayesian Criterion (SBC), and Central Process Unit time (CPU time). The introduced method outperformed the existing four algorithms in the aspect of robustness, accuracy, and sensitivity throughout the simulation process. Therefore, it has been proven that the proposed AIS algorithm effectively conformed to the RBFNN-2SATRA in relation to (or in terms of) the average value of training of RMSE rose up to 97.5%, SBC rose up to 99.9%, and CPU time by 99.8%. Moreover, the average value of testing in MAE was rose up to 78.5%, MAPE was rose up to 71.4%, and was capable of classifying a higher percentage (81.6%) of the test samples compared with the results for the GA, DE, PSO, and ABC algorithms. Full article

Amazon.com Inc. seeks alternative ways to improve manual transactions system of granting employees resources access in the field of data science. The work constructs a modified Artificial Neural Network (ANN) by incorporating a Discrete Hopfield Neural Network (DHNN) and Clonal Selection Algorithm (CSA) with 3-Satisfiability (3-SAT) logic to initiate an Artificial Intelligence (AI) model that executes optimization tasks for industrial data. The selection of 3-SAT logic is vital in data mining to represent entries of Amazon Employees Resources Access (AERA) via information theory. The proposed model employs CSA to improve the learning phase of DHNN by capitalizing features of CSA such as hypermutation and cloning process. This resulting the formation of the proposed model, as an alternative machine learning model to identify factors that should be prioritized in the approval of employees resources applications. Subsequently, reverse analysis method (SATRA) is integrated into our proposed model to extract the relationship of AERA entries based on logical representation. The study will be presented by implementing simulated, benchmark and AERA data sets with multiple performance evaluation metrics. Based on the findings, the proposed model outperformed the other existing methods in AERA data extraction. Full article

Human papilloma virus (HPV) is one of the most prevalent viral sexually transmitted diseases. The ability of HPV to induce malignancy in the anogenital tract and stomato-pharyngeal cavity is well documented. Moreover, HPV infection may also affect reproductive health and fertility. Although, the impact of HPV on female fertility has not been thoroughly studied it has been found also to have an impact on semen parameters. Relative information can be obtained from studies investigating the relationship between HPV and pregnancy success. Furthermore, there is an ongoing debate whether HPV alters the efficacy of assisted reproductive technologies. An association between HPV and assisted reproductive technologies (ART) programs has been reported. Nevertheless, due to conflicting data and the small number of existing studies further research is required. It remains to be clarified whether HPV detection and genotyping could be included in the diagnostic procedures in couples undergoing in vitro fertilization (IVF)/intrauterine insemination (IUI) treatments. Vaccination of both genders against HPV can reduce the prevalence of HPV infection and eliminate its implications on human fertility. The aim of the present mini-review is to reiterate the association between HPV and human fertility through a systematic literature review. Full article