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19 pages, 3259 KiB  
Article
Examining the Impact of National Planning on Rural Residents’ Disposable Income in China—The Case of Functional Zoning
by Junrong Ma, Chen Liu and Li Tian
Land 2025, 14(8), 1587; https://doi.org/10.3390/land14081587 (registering DOI) - 3 Aug 2025
Abstract
The growth of rural residents’ disposable income is essential for narrowing the income gap between urban and rural areas and promoting integrated development. This study explores how China’s National Main Functional Zoning Plan influences rural household income through its regulatory impact on construction [...] Read more.
The growth of rural residents’ disposable income is essential for narrowing the income gap between urban and rural areas and promoting integrated development. This study explores how China’s National Main Functional Zoning Plan influences rural household income through its regulatory impact on construction land expansion. Using data from county−level administrative units across China, the research identified the construction land regulation index as a key mediating variable linking zoning policy to changes in household income. By shifting the analytical perspective from a traditional urban–rural classification to a framework aligned with the National Main Functional Zoning Plan, the study reveals how spatial planning tools, particularly differentiated land quota allocations, influence household income. The empirical results confirm a structured causal chain in which zoning policy affects land development intensity, which in turn drives rural income growth. This relationship varies across different functional zones. In key development zones, strict land control limits income potential by constraining land supply. In main agricultural production zones, moderate regulatory control enhances land use efficiency and contributes to higher income levels. In key ecological function zones, ecological constraints require diverse approaches to value realization. The investigation contributes both theoretical and practical insights by elucidating the microeconomic effects of national spatial planning policies and offering actionable guidance for optimizing land use regulation to support income growth tailored to regional functions. Full article
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30 pages, 4622 KiB  
Article
Durability Analysis of Brick-Faced Clay-Core Walls in Traditional Residential Architecture in Quanzhou, China
by Yuhong Ding, Ruiming Guan, Li Chen, Jinxuan Wang, Yangming Zhang, Yili Fu and Canjin Zhang
Coatings 2025, 15(8), 909; https://doi.org/10.3390/coatings15080909 (registering DOI) - 3 Aug 2025
Abstract
This study analyzes the durability of brick-faced clay-core walls (BCWs) in the traditional residential architecture of Quanzhou—a UNESCO World Heritage City. Taking the northern gable of Ding Gongchen’s former residence as an example, the mechanical properties, microscopic structure, and changes in chemical symbol, [...] Read more.
This study analyzes the durability of brick-faced clay-core walls (BCWs) in the traditional residential architecture of Quanzhou—a UNESCO World Heritage City. Taking the northern gable of Ding Gongchen’s former residence as an example, the mechanical properties, microscopic structure, and changes in chemical symbol, oxides and minerals of the red bricks and clay-cores were analyzed using finite element mechanics analysis (FEM), scanning electron microscopy (SEM), X-ray fluorescence (XRF), and X-ray diffraction (XRD). The results indicate a triple mechanism: (1) The collaborative protection and reinforcement mechanism of “brick-wrapped-clay”. (2) The infiltration and destruction mechanism of external pollutants. (3) The material stability mechanism of silicate minerals. Therefore, the key to maintaining the durability of BCWs lies in the synergistic effect of brick and clay materials and the stability of silicate mineral materials, providing theoretical and methodological support for sustainable research into brick and clay constructions. Full article
13 pages, 1388 KiB  
Article
A Proof-of-Concept Study on Bioelectric-Based Biosensing for Prostate-Specific Antigen Detection in Serum Samples
by Georgios Giannakos, Sofia Marka, Konstantina Georgoulia, Spyridon Kintzios and Georgia Moschopoulou
Biosensors 2025, 15(8), 503; https://doi.org/10.3390/bios15080503 (registering DOI) - 3 Aug 2025
Abstract
Prostate cancer is among the most prevalent malignancies in men worldwide, underscoring the need for early and accurate diagnostic tools. This study presents a proof-of-concept and pilot clinical validation of a novel bioelectric impedance-based biosensor for the detection of prostate-specific antigen (PSA) in [...] Read more.
Prostate cancer is among the most prevalent malignancies in men worldwide, underscoring the need for early and accurate diagnostic tools. This study presents a proof-of-concept and pilot clinical validation of a novel bioelectric impedance-based biosensor for the detection of prostate-specific antigen (PSA) in human serum. The system integrates Molecular Identification through Membrane Engineering (MIME) with the xCELLigence real-time cell analysis platform, employing Vero cells electroinserted with anti-PSA antibodies. Optimization experiments identified 15,000 cells/well as the optimal configuration for impedance response. The biosensor exhibited specific, concentration-dependent changes in impedance upon exposure to PSA standard solutions and demonstrated significant differentiation between PSA-positive and PSA-negative human serum samples relative to the clinical threshold of 4 ng/mL. The biosensor offered rapid results within one minute, unlike standard immunoradiometric assay (IRMA), while showing strong diagnostic agreement. The system’s specificity, sensitivity, and reproducibility support its potential for integration into point-of-care screening workflows. This bioelectric assay represents one of the fastest PSA detection approaches reported to date and offers a promising solution for reducing overdiagnosis while improving clinical decision-making and patient outcomes. Full article
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11 pages, 1174 KiB  
Article
385 nm AlGaN Near-Ultraviolet Micro Light-Emitting Diode Arrays with WPE 30.18% Realized Using an AlN-Inserted Hole Spreading Enhancement Superlattice Electron Blocking Layer
by Qi Nan, Shuhan Zhang, Jiahao Yao, Yun Zhang, Hui Ding, Qian Fan, Xianfeng Ni and Xing Gu
Coatings 2025, 15(8), 910; https://doi.org/10.3390/coatings15080910 (registering DOI) - 3 Aug 2025
Abstract
In this work, we demonstrate high-efficiency 385 nm AlGaN-based near-ultraviolet micro light emitting diode (NUV-Micro LED) arrays. The epi structure is prepared using a novel AlN-inserted superlattice electrical blocking layer which enhances hole spreading in the p-type region significantly. The NUV-Micro LED arrays [...] Read more.
In this work, we demonstrate high-efficiency 385 nm AlGaN-based near-ultraviolet micro light emitting diode (NUV-Micro LED) arrays. The epi structure is prepared using a novel AlN-inserted superlattice electrical blocking layer which enhances hole spreading in the p-type region significantly. The NUV-Micro LED arrays in this work comprise 228 chips in parallel with wavelengths at 385 nm, and each single chip size is 15 × 30 μm2. Compared with conventional bulk AlGaN-based EBL structures, the NUV-Micro LED arrays that implemented the new hole spreading enhanced superlattice electrical blocking layer (HSESL-EBL) structure proposed in this work had a remarkable increase in light output power (LOP) at current density, increasing the range down from 0.02 A/cm2 to as high as 97 A/cm2. The array’s light output power is increased up to 1540% at the lowest current density 0.02 A/cm2, and up to 58% at the highest current density 97 A/cm2, measured under room temperature (RT); consequently, the WPE is increased from 13.4% to a maximum of 30.18%. This AlN-inserted HESEL-EBL design significantly enhances both the lateral expansion efficiency and the hole injection efficiency into the multi quantum well (MQW) in the arrays, improving the concentration distribution of the holes in MQW while maintaining good suppression of electron leakage. The array’s efficiency droop has also been greatly reduced. Full article
11 pages, 614 KiB  
Article
Evaluation of Race Pace Using Critical Swimming Speed During 10 km Open-Water Swimming Competition
by Yasunori Fujito, Tomomi Fujimoto, Reira Hara, Ryuhei Yoshida and Kazuo Funato
J. Funct. Morphol. Kinesiol. 2025, 10(3), 302; https://doi.org/10.3390/jfmk10030302 (registering DOI) - 3 Aug 2025
Abstract
Background: Estimating race times for open-water swimming based on pool swimming times could be useful for talent identification and training optimisation. We aimed to compare the swimming speeds of the world’s top and other swimmers in the 2023 Aquatics Championship men’s 10 [...] Read more.
Background: Estimating race times for open-water swimming based on pool swimming times could be useful for talent identification and training optimisation. We aimed to compare the swimming speeds of the world’s top and other swimmers in the 2023 Aquatics Championship men’s 10 km OWS race. Methods: Sixty-five swimmers were divided into four groups: G1 (1st–10th positions), G2 (11st–30th positions), G3 (31st–47th positions), and G4 (48th–65th positions). Swimming speed, stroke frequency (SF), and stroke length (SL) for each lap (laps 1–6) were recorded. Critical speed (CS) was calculated from each participant’s personal best times in the 400, 800, and 1500 m freestyle events in the pool. Swimming speed against CS was calculated (%CS). Results: The top performance group (G1) maintained their swimming speed from beginning (lap 1, 1.53 m/s) to end (lap 6, 1.50 m/s), at 92.7 ± 1.9% of CS, characterised by longer SL (1.26 m) and lower SF (72.86 rpm). G3 and G4 were unable to maintain their swimming speed, which decreased from G3: 97.64 ± 1.62% and G4: 96.10 ± 1.96% of CS at lap 1 to G3: 88.39 ± 3.78% and G4: 85.13 ± 5.04% at lap 6. This reduction in swimming speed is consistent with the increased reliance on anaerobic metabolism reported in previous studies under similar conditions. Conclusions: Race pacing for maintaining speeds of 92%CS throughout the race could be an important resilient index in open-water swimming. %CS might be a useful index for estimating the athletic performance level in open-water swimming. Full article
(This article belongs to the Section Athletic Training and Human Performance)
19 pages, 2276 KiB  
Article
Segmentation of Stone Slab Cracks Based on an Improved YOLOv8 Algorithm
by Qitao Tian, Runshu Peng and Fuzeng Wang
Appl. Sci. 2025, 15(15), 8610; https://doi.org/10.3390/app15158610 (registering DOI) - 3 Aug 2025
Abstract
To tackle the challenges of detecting complex cracks on large stone slabs with noisy textures, this paper presents the first domain-optimized framework for stone slab cracks, an improved semantic segmentation model (YOLOv8-Seg) synergistically integrating U-NetV2, DSConv, and DySample. The network uses the lightweight [...] Read more.
To tackle the challenges of detecting complex cracks on large stone slabs with noisy textures, this paper presents the first domain-optimized framework for stone slab cracks, an improved semantic segmentation model (YOLOv8-Seg) synergistically integrating U-NetV2, DSConv, and DySample. The network uses the lightweight U-NetV2 backbone combined with dynamic feature recalibration and multi-scale refinement to better capture fine crack details. The dynamic up-sampling module (DySample) helps to adaptively reconstruct curved boundaries. In addition, the dynamic snake convolution head (DSConv) improves the model’s ability to follow irregular crack shapes. Experiments on the custom-built ST stone crack dataset show that YOLOv8-Seg achieves an mAP@0.5 of 0.856 and an mAP@0.5–0.95 of 0.479. The model also reaches a mean intersection over union (MIoU) of 79.17%, outperforming both baseline and mainstream segmentation models. Ablation studies confirm the value of each module. Comparative tests and industrial validation demonstrate stable performance across different stone materials and textures and a 30% false-positive reduction in real production environments. Overall, YOLOv8-Seg greatly improves segmentation accuracy and robustness in industrial crack detection on natural stone slabs, offering a strong solution for intelligent visual inspection in real-world applications. Full article
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18 pages, 7618 KiB  
Article
A Comparative Analysis of Axial Bearing Behaviour in Steel Pipe Piles and PHC Piles for Port Engineering
by Runze Zhang, Yizhi Liu, Lei Wang, Weiming Gong and Zhihui Wan
Buildings 2025, 15(15), 2738; https://doi.org/10.3390/buildings15152738 (registering DOI) - 3 Aug 2025
Abstract
This paper addresses the critical challenge of selecting suitable pile foundations in port engineering by systematically investigating the axial bearing behavior of large-diameter steel pipe piles and prestressed high-strength concrete (PHC) piles. The study integrates both numerical simulations and field tests within the [...] Read more.
This paper addresses the critical challenge of selecting suitable pile foundations in port engineering by systematically investigating the axial bearing behavior of large-diameter steel pipe piles and prestressed high-strength concrete (PHC) piles. The study integrates both numerical simulations and field tests within the context of the Yancheng Dafeng Port Security Facilities Project. A self-balanced static load numerical model for PHC piles was developed using Plaxis 3D, enabling the simulation of load-displacement responses, axial force transfer, and side resistance distribution. The accuracy of the model was verified through a comparison with field static load test data. With the verified model parameters, the internal force distribution of steel pipe piles was analysed by modifying material properties and adjusting boundary conditions. A comparative analysis of the two pile types was conducted under identical working conditions. The results reveal that the ultimate bearing capacities of the 1# steel pipe pile and the 2# PHC pile are 6734 kN and 6788 kN, respectively. Despite the PHC pile having a 20% larger diameter, its ultimate bearing capacity is comparable to that of the steel pipe pile, suggesting a more efficient utilisation of material strength in the latter. Further numerical simulations indicate that, under the same working conditions, the ultimate bearing capacity of the steel pipe pile exceeds that of the PHC pile by 18.43%. Additionally, the axial force distribution along the steel pipe pile shaft is more uniform, and side resistance is mobilised more effectively. The reduction in side resistance caused by construction disturbances, combined with the slenderness ratio (L/D = 41.7) of the PHC pile, results in 33.87% of the pile’s total bearing capacity being attributed to tip resistance. The findings of this study provide crucial insights into the selection of optimal pile types for terminal foundations, considering factors such as bearing capacity, environmental conditions, and economic viability. Full article
(This article belongs to the Section Building Structures)
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10 pages, 789 KiB  
Communication
Female Sex Workers in the Amazon Region of Brazil Are at High Risk of Chlamydia trachomatis Infection: A Retrospective Study
by Leonardo Gabriel Campelo Pinto de Figueiredo, Paula do Socorro de Oliveira da Costa Laurindo, Daniela Assunção Pantoja, Maurimélia Mesquita da Costa, Diogo Oliveira de Araújo, Felipe Bonfim Freitas, Jacqueline Cortinhas Monteiro, Ricardo Roberto de Souza Fonseca, Rosimar Neris Martins Feitosa, Rogério Valois Laurentino, Leonardo Miranda dos Santos, Aldemir Branco Oliveira-Filho and Luiz Fernando Almeida Machado
Microorganisms 2025, 13(8), 1815; https://doi.org/10.3390/microorganisms13081815 (registering DOI) - 3 Aug 2025
Abstract
Background: Chlamydia trachomatis is the most prevalent bacterial sexually transmitted infection (STI) globally, linked to severe complications such as pelvic inflammatory disease and infertility. In the Brazilian Amazon, socioeconomic vulnerability and the absence of screening policies exacerbate risks, particularly among female sex workers [...] Read more.
Background: Chlamydia trachomatis is the most prevalent bacterial sexually transmitted infection (STI) globally, linked to severe complications such as pelvic inflammatory disease and infertility. In the Brazilian Amazon, socioeconomic vulnerability and the absence of screening policies exacerbate risks, particularly among female sex workers (FSWs). Objective: This study aimed to determine the seroprevalence of anti-C. trachomatis IgG antibodies among FSWs in five municipalities of Pará State, Brazilian Amazon, and identify epidemiological factors associated with infection. Methods: A retrospective cross-sectional study (2005–2007) included 348 FSWs recruited via convenience sampling. Sociodemographic and behavioral data were collected through questionnaires, and blood samples were analyzed by ELISA for anti-C. trachomatis IgG. Statistical analyses included Fisher’s exact tests, odds ratios (ORs), and 95% confidence intervals (CIs), using SPSS 21.0. Results: Overall seroprevalence was 93.9% (327/348; 95% CI: 83.1–90%). Significant associations included a household income of 1–3 minimum wages (98.4%; p = 0.0002), sexual partners from the same region (98.8%; p = 0.0421), and age >42 years (96.3%). Most reported inconsistent condom use (43.7%), multiple monthly partners (54.6%), and illicit drug use (53.4%). Discussion: The extremely high seroprevalence reflects chronic C. trachomatis exposure, driven by socioeconomic deprivation and limited healthcare access. Comparisons with global data underscore the urgent need for screening policies, absent in Brazil for FSWs, and highlight the vulnerability of this population. Conclusions: The findings reveal an alarming burden of C. trachomatis exposure among Amazonian FSWs. Integrated strategies, including routine screening, sexual health education, and inclusion of FSWs in priority health programs, are critical to reduce transmission and associated complications. Full article
(This article belongs to the Special Issue Chlamydiae and Chlamydia-Like Infections)
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19 pages, 4313 KiB  
Article
Integrating Clinical and Imaging Markers for Survival Prediction in Advanced NSCLC Treated with EGFR-TKIs
by Thanika Ketpueak, Phumiphat Losuriya, Thanat Kanthawang, Pakorn Prakaikietikul, Lalita Lumkul, Phichayut Phinyo and Pattraporn Tajarernmuang
Cancers 2025, 17(15), 2565; https://doi.org/10.3390/cancers17152565 (registering DOI) - 3 Aug 2025
Abstract
Background: Epidermal growth factor receptor (EGFR) mutations are presented in approximately 50% of East Asian populations with advanced non-small cell lung cancer (NSCLC). While EGFR-tyrosine kinase inhibitors (TKIs) are the standard treatment, patient outcomes are also influenced by host-related factors. This study aimed [...] Read more.
Background: Epidermal growth factor receptor (EGFR) mutations are presented in approximately 50% of East Asian populations with advanced non-small cell lung cancer (NSCLC). While EGFR-tyrosine kinase inhibitors (TKIs) are the standard treatment, patient outcomes are also influenced by host-related factors. This study aimed to investigate clinical and radiological factors associated with early mortality and develop a prognostic prediction model in advanced EGFR-mutated NSCLC. Methods: A retrospective cohort was conducted in patients with EGFR-mutated NSCLC treated with first line EGFR-TKIs from January 2012 to October 2022 at Chiang Mai University Hospital. Clinical data and radiologic findings at the initiation of treatment were analyzed. A multivariable flexible parametric survival model was used to determine the predictors of death at 18 months. The predicted survival probabilities at 6, 12, and 18 months were estimated, and the model performance was evaluated. Results: Among 189 patients, 84 (44.4%) died within 18 months. Significant predictors of mortality included body mass index <18.5 or ≥23, bone metastasis, neutrophil-to-lymphocyte ratio ≥ 5, albumin-to-globulin ratio < 1, and mean pulmonary artery diameter ≥ 29 mm. The model demonstrated good performance (Harrell’s C-statistic = 0.72; 95% CI: 0.66–0.78). Based on bootstrap internal validation, the optimism-corrected Harrell’s C-statistic was 0.71 (95% CI: 0.71–0.71), derived from an apparent C-statistic of 0.75 (95% CI: 0.74–0.75) and an estimated optimism of 0.04 (95% CI: 0.03–0.04). Estimated 18-month survival ranged from 87.1% in those without risk factors to 2.1% in those with all predictors. A web-based tool was developed for clinical use. Conclusions: The prognostic model developed from fundamental clinical and radiologic parameters demonstrated promising utility in predicting 18-month mortality in patients with advanced EGFR-mutated NSCLC receiving first-line EGFR-TKI therapy. Full article
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17 pages, 4783 KiB  
Article
Empirical Investigation of the Structural Response of Super-Span Soil–Steel Arches During Backfilling
by Bartłomiej Kunecki
Materials 2025, 18(15), 3650; https://doi.org/10.3390/ma18153650 (registering DOI) - 3 Aug 2025
Abstract
This paper presents field investigations of a corrugated steel soil–steel arch structure with a span of 25.7 m and a rise of 9.0 m—currently the largest single-span structure of its kind in Europe. The structure, serving as a wildlife crossing along the DK16 [...] Read more.
This paper presents field investigations of a corrugated steel soil–steel arch structure with a span of 25.7 m and a rise of 9.0 m—currently the largest single-span structure of its kind in Europe. The structure, serving as a wildlife crossing along the DK16 expressway in northeastern Poland, was constructed using deep corrugated steel plates (500 mm× 237 mm) made from S315MC steel, without additional reinforcements such as stiffening ribs or geosynthetics. The study focused on monitoring the structural behavior during the critical backfilling phase. Displacements and strains were recorded using 34 electro-resistant strain gauges and a geodetic laser system at successive backfill levels, with particular attention to the loading stage at the crown. The measured results were compared with predictions based on the Swedish Design Method (SDM). The SDM equations did not accurately predict internal forces during backfilling. At the crown level, bending moments and axial forces were overestimated by approximately 69% and 152%, respectively. At the final backfill level, the SDM underestimated bending moments by 55% and overestimated axial forces by 90%. These findings highlight limitations of current design standards and emphasize the need for revised analytical models and long-term monitoring of large-span soil–steel structures. Full article
(This article belongs to the Section Construction and Building Materials)
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25 pages, 4386 KiB  
Article
Design and Testing of a Pneumatic Jujube Harvester
by Huaming Hou, Wei Niu, Qixian Wen, Hairui Yang, Jianming Zhang, Rui Zhang, Bing Xv and Qingliang Cui
Agronomy 2025, 15(8), 1881; https://doi.org/10.3390/agronomy15081881 (registering DOI) - 3 Aug 2025
Abstract
Jujubes have a beautiful taste, and high nutritional and economic value. The planting area of dwarf and densely planted jujubes is large and shows an increasing trend; however, the mechanization level and efficiency of fresh jujube harvesting are low. For this reason, our [...] Read more.
Jujubes have a beautiful taste, and high nutritional and economic value. The planting area of dwarf and densely planted jujubes is large and shows an increasing trend; however, the mechanization level and efficiency of fresh jujube harvesting are low. For this reason, our research group conducted a study on mechanical harvesting technology for fresh jujubes. A pneumatic jujube harvester was designed. This harvester is composed of a self-regulating picking mechanism, a telescopic conveying pipe, a negative pressure generator, a cleaning mechanism, a double-chamber collection box, a single-door shell, a control assembly, a generator, a towing mobile chassis, etc. During the harvest, the fresh jujubes on the branches are picked under the combined effect of the flexible squeezing of the picking roller and the suction force of the negative pressure air flow. They then enter the cleaning mechanism through the telescopic conveying pipe. Under the combined effect of the upper and lower baffles of the cleaning mechanism and the negative-pressure air flow, the fresh jujubes are separated from impurities such as jujube leaves and branches. The clean fresh jujubes fall into the collection box. We considered the damage rate of fresh jujubes, impurity rate, leakage rate, and harvesting efficiency as the indexes, and the negative-pressure suction wind speed, picking roller rotational speed, and the inclination angle of the upper and lower baffles of the cleaning and selection machinery as the test factors, and carried out the harvesting test of fresh jujubes. The test results show that when the negative-pressure suction wind speed was 25 m/s, the picking roller rotational speed was 31 r/min, and the inclination angles of the upper and lower baffle plates for cleaning and selecting were −19° and 19.5°, respectively, the breakage rate of fresh jujube harvesting was 0.90%, the rate of impurity was 1.54%, the rate of leakage was 2.59%, and the efficiency of harvesting was 73.37 kg/h, realizing the high-efficiency and low-loss harvesting of fresh jujubes. This study provides a reference for the research and development of fresh jujube mechanical harvesting technology and equipment. Full article
(This article belongs to the Section Precision and Digital Agriculture)
23 pages, 343 KiB  
Article
How Do China’s OFDI Motivations Affect the Bilateral GVC Relationship and Sustainable Global Economy?
by Min Wang
Sustainability 2025, 17(15), 7049; https://doi.org/10.3390/su17157049 (registering DOI) - 3 Aug 2025
Abstract
The purpose of this paper is to analyze how China’s outward foreign direct investment (OFDI), driven by different motivations, affects the bilateral global value chain (GVC) relationship between the home country (China) and host countries, evaluating both bilateral GVC trade value and relative [...] Read more.
The purpose of this paper is to analyze how China’s outward foreign direct investment (OFDI), driven by different motivations, affects the bilateral global value chain (GVC) relationship between the home country (China) and host countries, evaluating both bilateral GVC trade value and relative GVC positions. Employing the OECD Trade in Value Added (TiVA) database combined with Chinese listed firm data, we found the following results: (1) Strategic asset-seeking OFDI strengthens the GVC relationship between China and host countries while enhancing China’s GVC position relative to host countries. (2) Efficiency-seeking OFDI increases the domestic value-added exported from host countries to China but does not improve China’s relative GVC position. (3) Natural resource-seeking OFDI enhances bilateral GVC trade volumes but has no significant impact on the relative GVC positions of China and host countries. (4) China’s OFDI, not driven by these motivations, generates a trade substitution effect between home and host countries. We also examined the heterogeneity of these effects. Our findings suggest that China’s OFDI fosters equitable and sustainable international cooperation, supports mutually beneficial GVC trade and host-country economic growth, and therefore, progresses toward Sustainable Development Goal (SDG) 8. Full article
11 pages, 560 KiB  
Article
Expression of 15-PGDH Regulates Body Weight and Body Size by Targeting JH in Honeybees (Apis mellifera)
by Xinying Qu, Xinru Zhang, Hanbing Lu, Lingjun Xin, Ran Liu and Xiao Chen
Life 2025, 15(8), 1230; https://doi.org/10.3390/life15081230 (registering DOI) - 3 Aug 2025
Abstract
Honeybees (Apis mellifera) are pollinators for most crops in nature and a core species for the production of bee products. Body size and body weight are crucial breeding traits, as colonies possessing individuals with large body weight tend to be healthier [...] Read more.
Honeybees (Apis mellifera) are pollinators for most crops in nature and a core species for the production of bee products. Body size and body weight are crucial breeding traits, as colonies possessing individuals with large body weight tend to be healthier and exhibit high productivity. In this study, small interfering RNA (siRNA) targeting 15-Hydroxyprostaglandin dehydrogenase (15-PGDH) was incorporated into the feed for feeding worker bee larvae, thereby achieving the silencing of this gene’s expression. The research further analyzed the impact of the RNA expression level of the 15-PGDH gene on the juvenile hormone (JH) titer and its subsequent effects on the body weight and size of worker bees. The results show that inhibiting the expression of 15-PGDH in larvae could significantly increase JH titer, which in turn led to an increase in the body weight of worker bees (1.13-fold higher than that of the control group reared under normal conditions (CK group); p < 0.01; SE: 7.85) and a significant extension in femur (1.08-fold longer than that of the CK group; p < 0.01; SE: 0.18). This study confirms that 15-PGDH can serve as a molecular marker related to body weight and size in honey bees, providing an important basis for molecular marker-assisted selection in honey bee breeding. Full article
(This article belongs to the Section Animal Science)
14 pages, 3361 KiB  
Article
Bacteremia Caused by a Putative Novel Species in the Genus Erwinia: A Case Report and Genomic Analysis
by Jiwoo Lee, Taek Soo Kim, Hyunwoong Park and Jae Hyeon Park
Life 2025, 15(8), 1227; https://doi.org/10.3390/life15081227 (registering DOI) - 3 Aug 2025
Abstract
We report a case of catheter-associated bloodstream infection caused by a putative novel species in the genus Erwinia, identified using whole-genome sequencing (WGS). A female adolescent receiving long-term home parenteral nutrition via a central venous catheter (CVC) presented with a fever. Gram-negative [...] Read more.
We report a case of catheter-associated bloodstream infection caused by a putative novel species in the genus Erwinia, identified using whole-genome sequencing (WGS). A female adolescent receiving long-term home parenteral nutrition via a central venous catheter (CVC) presented with a fever. Gram-negative rods were isolated from two CVC-derived blood culture sets, while peripheral cultures remained negative. Conventional identification methods, including VITEK 2, Phoenix M50, MALDI-TOF MS, and 16S rRNA and rpoB gene sequencing, failed to achieve species-level identification. WGS was performed on the isolate using Illumina MiSeq. Genomic analysis revealed a genome size of 5.39 Mb with 56.8% GC content and high assembly completeness. The highest average nucleotide identity (ANI) was 90.3% with Pantoea coffeiphila, and ≤85% with known Erwinia species, suggesting that it represents a distinct taxon. Phylogenetic analyses placed the isolate within the Erwinia clade but separate from any known species. Antimicrobial susceptibility testing showed broad susceptibility. This case highlights the utility of WGS for the identification of rare or novel organisms not captured by conventional methods and expands the clinical spectrum of Erwinia species. While the criteria for species delineation were met, the phenotypic characterization remains insufficient to formally propose a new species. Full article
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Article
Sustainable Tourism in Protected Areas: Comparative Governance and Lessons from Tara and Triglav National Parks
by Stefana Matović, Suzana Lović Obradović and Tamara Gajić
Sustainability 2025, 17(15), 7048; https://doi.org/10.3390/su17157048 (registering DOI) - 3 Aug 2025
Abstract
This paper investigates how governance frameworks shape sustainable tourism outcomes in protected areas by comparing Tara National Park (Serbia) and Triglav National Park (Slovenia). Both parks, established in 1981 and classified under IUCN Category II, exhibit rich biodiversity and mountainous terrain but differ [...] Read more.
This paper investigates how governance frameworks shape sustainable tourism outcomes in protected areas by comparing Tara National Park (Serbia) and Triglav National Park (Slovenia). Both parks, established in 1981 and classified under IUCN Category II, exhibit rich biodiversity and mountainous terrain but differ markedly in governance structures, institutional integration, and local community engagement. Using a qualitative, indicator-based methodology, this research evaluates ecological, economic, and social dimensions of sustainability. The findings reveal that Triglav NP demonstrates higher levels of participatory governance, tourism integration, and educational outreach, while Tara NP maintains stricter ecological protection with less inclusive management. Triglav’s zoning model, community council, and economic alignment with regional development policies contribute to stronger sustainability outcomes. Conversely, Tara NP’s centralized governance and infrastructural gaps constrain its potential despite its significant conservation value. This study highlights the importance of adaptive, inclusive governance in achieving the Sustainable Development Goals (SDGs) within protected areas. It concludes that hybrid approaches, combining legal rigor with participatory flexibility, can foster resilience and sustainability in ecologically sensitive regions. Full article
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