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Keywords = Picea abies (L.) Karst.

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15 pages, 27708 KiB  
Article
Defoliation of Norway Spruce by Spruce Budworm (Lepidoptera: Tortricidae) and Protection Using Bacillus thuringiensis
by Alvaro Fuentealba, Richard Berthiaume, Simon Fortier, Louis Morneau and Éric Bauce
Forests 2025, 16(7), 1056; https://doi.org/10.3390/f16071056 - 25 Jun 2025
Viewed by 311
Abstract
Norway spruce (Picea abies (L.) Karst.) has been widely planted beyond its natural range due to its fast growth rate and valuable wood. In Québec, over 200 million seedlings have been planted since 1964. Several of these plantations are now facing a [...] Read more.
Norway spruce (Picea abies (L.) Karst.) has been widely planted beyond its natural range due to its fast growth rate and valuable wood. In Québec, over 200 million seedlings have been planted since 1964. Several of these plantations are now facing a new potential threat, i.e., spruce budworm (Choristoneura fumiferana (Clem.)) infestations. Despite contrasting results, Norway and white spruce (P. glauca [Moench] Voss) apparently sustain a similar degree of budworm defoliation. The main study objective is to quantify defoliation in Norway spruce caused by spruce budworm. We also evaluate the efficacy of Bacillus thuringiensis Berliner spp. kurstaki (Btk) in protecting this exotic host tree. Annual defoliation was assessed in plantations of Norway, white, and black spruce (P. mariana [Mill.] BSP) between 2018 and 2022 in the Bas-Saint-Laurent region. Additional surveys were conducted in Norway and white spruce plantations in the Gaspésie and Côte-Nord to evaluate Btk efficacy. We show that both species exhibit similar defoliation levels, though Norway spruce sometimes sustains greater damage (e.g., 35% vs. 10% in 2019). Btk formulations showed low efficacy in protecting Norway spruce foliage (≥49.32% defoliation in treated plantations). Further studies are needed to understand factors influencing Btk efficacy on this host. Full article
(This article belongs to the Special Issue Monitoring and Control of Forest Pests)
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22 pages, 2033 KiB  
Article
The Mechanical Properties of Laminated Veneer Products from Different Stands of Douglas Fir and Norway Spruce in Germany
by Tobias Krenn, Dirk Berthold, Nina Ritter and Carsten Mai
Forests 2025, 16(7), 1040; https://doi.org/10.3390/f16071040 - 21 Jun 2025
Viewed by 288
Abstract
The relationship between silvicultural strategies, manifested in the thinning method and rotation age on sites with different water supply, and the mechanical properties of engineered wood products plywood and laminated veneer lumber has been analyzed. Sample logs from five German sites of Norway [...] Read more.
The relationship between silvicultural strategies, manifested in the thinning method and rotation age on sites with different water supply, and the mechanical properties of engineered wood products plywood and laminated veneer lumber has been analyzed. Sample logs from five German sites of Norway spruce (Picea abies (L.) Karst.) and Douglas fir (Pseudotsuga menziesii (M.) Franco) have been rotary-peeled and processed into boards with a phenol–resorcinol–formaldehyde adhesive to evaluate their performance under flexural, tensile, and compressive loads. Satisfactory coefficients of determination were reached for Norway spruce in regard to the silvicultural framework and the tree characteristics of slenderness and crown base height. Douglas fir products did not achieve comparable determination due to high variance within boards and stands but did achieve significantly better mechanical properties. Norway spruce was observed to be more responsive to thinning measures, while the effect of different thinning regimes was not evident for Douglas fir. The on-site evaluation of Douglas fir stands for veneer product quality based on silvicultural parameters and tree characteristics was shown to be inconclusive, with its naturally higher wood density being the decisive constant. Full article
(This article belongs to the Special Issue Wood Properties: Strength, Density, Hardness)
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14 pages, 1594 KiB  
Article
Effect of Lead on the Physiological Parameters and Elemental Composition of Pinus sylvestris L. and Picea abies (L.) H. Karst Seedlings
by Andrea Pogányová, Djordje P. Božović, Martin Bačkor, Michal Goga, Marián Tomka and Marko S. Sabovljević
Forests 2025, 16(6), 990; https://doi.org/10.3390/f16060990 - 11 Jun 2025
Viewed by 315
Abstract
Lead (Pb) pollution poses a long-term threat to forest ecosystems, particularly in mountainous areas affected by atmospheric deposition. This study examined the physiological and biochemical responses of juvenile Pinus sylvestris L. and Picea abies (L.) H. Karst seedlings to low concentrations of lead [...] Read more.
Lead (Pb) pollution poses a long-term threat to forest ecosystems, particularly in mountainous areas affected by atmospheric deposition. This study examined the physiological and biochemical responses of juvenile Pinus sylvestris L. and Picea abies (L.) H. Karst seedlings to low concentrations of lead nitrate during early development. Treatments simulated environmentally relevant Pb exposure and focused on pigment composition, oxidative stress markers, soluble protein and proline levels, and elemental content. Both species exhibited hormetic stimulation of photosynthetic pigments at lower Pb concentrations. In P. sylvestris, this effect declined at the highest dose, whereas P. abies maintained pigment levels, suggesting stronger regulatory control. Pb exposure reduced soluble proteins and induced species-specific alterations in MDA and proline levels. Correlation analysis revealed a well-integrated stress response in P. abies, while P. sylvestris showed a more fragmented pattern. Elemental analysis confirmed Pb accumulation primarily in roots, with higher levels in P. sylvestris. Both species experienced reduced root Mg, K, and Mn, indicating ionic imbalance due to Pb2+ interference. Zn content increased in P. sylvestris but decreased in P. abies, possibly reflecting differences in uptake regulation. These species-specific responses support the hypothesis that P. abies activates more effective defense mechanisms against Pb toxicity, while P. sylvestris exhibits a stronger physiological stress response. Full article
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26 pages, 4438 KiB  
Article
Ecology, Floristic–Vegetational Features, and Future Perspectives of Spruce Forests Affected by Ips typographus: Insight from the Southern Alps
by Luca Giupponi, Riccardo Panza, Davide Pedrali, Stefano Sala and Annamaria Giorgi
Plants 2025, 14(11), 1681; https://doi.org/10.3390/plants14111681 - 31 May 2025
Viewed by 687
Abstract
In recent years, many spruce (Picea abies (L.) H. Karst., Pinaceae) forests have been severely affected by bark beetle (Ips typographus L., Coleoptera: Curculionidae) outbreaks in the Southern Alps, but their ecological impacts remain poorly studied. We analyzed the distribution, ecological, [...] Read more.
In recent years, many spruce (Picea abies (L.) H. Karst., Pinaceae) forests have been severely affected by bark beetle (Ips typographus L., Coleoptera: Curculionidae) outbreaks in the Southern Alps, but their ecological impacts remain poorly studied. We analyzed the distribution, ecological, and floristic–vegetational characteristics of forests recently affected by the bark beetle in the upper basin of the Oglio River (Northern Italy) and developed a MaxEnt model to map forests with a bioclimate more prone to severe insect attacks in the coming decades. The results showed that the spruce forests affected by the bark beetle are located exclusively in the submountain and mountain belts (below 1600 m a.s.l.) and that 85% of them are found in areas with high annual solar radiation (>3500 MJ m−2). The predictive model for areas susceptible to severe bark beetle attacks proved highly accurate (AUC = 0.91) and was primarily defined by the mean temperature of the dry winter quarter (contribution: 80.1%), with values between −2.5 and 2.5 °C being particularly suitable for the pest. According to the model, more than 58% of the current spruce forests in the study area will exhibit high susceptibility (probability > 0.7) to severe bark beetle attacks by 2080. The floristic–vegetational and ecological analysis of plant communities of 11 bark beetle-affected areas indicated that more thermophilic and significantly different forest communities (in both floristic and physiognomic terms) are expected to develop compared to those of pre-disturbance. Furthermore, the high coverage of spruce snags/standing dead trees appears to accelerate plant succession, enabling the establishment of mature forest communities in a shorter time frame. Full article
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2 pages, 523 KiB  
Correction
Correction: Katrevičs et al. Forest Soil Fungal Diversity in Stands of Norway Spruce (Picea abies (L.) Karst.) of Different Ages. Forests 2025, 16, 500
by Juris Katrevičs, Krišs Bitenieks, Āris Jansons, Baiba Jansone and Dainis Edgars Ruņģis
Forests 2025, 16(6), 874; https://doi.org/10.3390/f16060874 - 22 May 2025
Viewed by 226
Abstract
In the original publication [...] Full article
(This article belongs to the Section Forest Soil)
15 pages, 2754 KiB  
Article
The Impact of Seed Treatment with Cold Plasma on Antioxidants, Sugars, and Pigments in Needles of Norway Spruce Is Genotype-Dependent
by Ieva Čėsnienė, Vytautas Čėsna, Vida Mildažienė, Diana Miškelytė, Dorotėja Vaitiekūnaitė and Vaida Sirgedaitė-Šėžienė
Plants 2025, 14(9), 1404; https://doi.org/10.3390/plants14091404 - 7 May 2025
Viewed by 630
Abstract
Forests face increasing threats due to climate change and anthropogenic pressures, exacerbating plant stress and disease susceptibility. Norway spruce (Picea abies (L.) H. Karst.), a key conifer species in European forestry, is particularly vulnerable. Developing innovative seed treatments to enhance tree resilience [...] Read more.
Forests face increasing threats due to climate change and anthropogenic pressures, exacerbating plant stress and disease susceptibility. Norway spruce (Picea abies (L.) H. Karst.), a key conifer species in European forestry, is particularly vulnerable. Developing innovative seed treatments to enhance tree resilience is crucial for sustainable forest management. Despite the growing interest in cold plasma (CP) technology for seed treatment, research on its long-term effects on trees, particularly Norway spruce, remains scarce. This study aimed to investigate the effects of pre-sowing CP treatment on Norway spruce seeds from 10 half-sib families over two vegetation seasons. Results indicate that CP treatment influenced key physiological and biochemical parameters in a genotype-specific and treatment duration-dependent manner (1 or 2 min). In some cases, CP-treated seedlings exhibited increased chlorophyll levels (e.g., increased chlorophyll a by up to 49% in some genotypes treated with CP for 1 min, and by up to 35% in those treated with CP for 2 min), reduced malondialdehyde (MDA) content in second-year samples (by up to 52% in some genotypes), and enhanced production of phenolics (by up to 21% in some genotypes in both treatment groups), suggesting improved stress tolerance. The 541 half-sib family is particularly noteworthy, as first-year seedlings exhibited increased levels of chlorophylls, flavonoids, and total phenols following a 2 min treatment. In contrast, second-year seedlings of the same family showed an increase in flavonoids and a reduction in MDA levels compared to the control, indicating a sustained and possibly age-modulated physiological response to CP exposure (2 min). However, responses varied across genetic backgrounds, highlighting the importance of genotype in determining treatment efficacy. These findings underscore the potential of CP technology as a tool for improving Norway spruce resilience and inform future strategies for seed enhancement in forestry. Full article
(This article belongs to the Special Issue Development of Woody Plants)
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26 pages, 7042 KiB  
Article
Thermodynamic Stability of Clay Minerals in Boreal Forest Soil and Its Relationship to the Properties of Soil Organic Matter
by Igor V. Danilin, Yulia G. Izosimova, Ruslan A. Aimaletdinov and Inna I. Tolpeshta
Minerals 2025, 15(4), 430; https://doi.org/10.3390/min15040430 - 20 Apr 2025
Viewed by 435
Abstract
This paper assesses the thermodynamic stability of clay minerals in the upper organo-mineral horizon of podzolic soil, as well as in the rhizosphere of Norway spruce (Picea abies (L.) H. Karst.) and Norway maple (Acer platanoides L.). Moreover, it determines the [...] Read more.
This paper assesses the thermodynamic stability of clay minerals in the upper organo-mineral horizon of podzolic soil, as well as in the rhizosphere of Norway spruce (Picea abies (L.) H. Karst.) and Norway maple (Acer platanoides L.). Moreover, it determines the impact of soil organic matter on the thermodynamic stability of clay minerals. Calculations of ΔGf and the saturation index (SI) for clay minerals in laboratory experiments simulating soil conditions without soil moisture outflow allowed us to find out that the thermodynamic stability of clay minerals decreased in the series kaolinite > illite > vermiculite > chlorite. In the rhizosphere of spruce, kaolinite, vermiculite and illite have the lowest, and in the soil under maple-the highest thermodynamic stability, which is associated with differences in the properties of soil organic matter of rhizospheres of different tree species. Laboratory experiments on the sorption of soil humic acid (HA) on clay minerals demonstrated that sorbed HA decreased the thermodynamic stability of biotite and increased the thermodynamic stability of kaolinite and muscovite. Thermodynamic stability of clay minerals decreased with increased proportion of sorbed thermolabile organic matter. Full article
(This article belongs to the Section Environmental Mineralogy and Biogeochemistry)
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16 pages, 5358 KiB  
Article
Impact of Climate on the Growth and Yield of the Main Tree Species from Romania Using Dendrochronological Data
by Marin Gheorghe and Bogdan M. Strimbu
Plants 2025, 14(8), 1234; https://doi.org/10.3390/plants14081234 - 18 Apr 2025
Viewed by 500
Abstract
National Forest Inventories (NFIs) offer a comprehensive and consistent dataset for forest analysis, enabling the refinement of growth and yield models by integrating regional environmental factors. This study investigates the influence of climate on the growth of three dominant tree species in Romania: [...] Read more.
National Forest Inventories (NFIs) offer a comprehensive and consistent dataset for forest analysis, enabling the refinement of growth and yield models by integrating regional environmental factors. This study investigates the influence of climate on the growth of three dominant tree species in Romania: Norway spruce (Picea abies L. Karst), European beech (Fagus sylvatica L.), and Sessile oak (Quercus petraea (Matt.) Liebl). Increment core analysis revealed a general increase in diameter growth since 1960, partially correlated with temperature trends. Repeated measures analysis confirmed significant variations in radial growth across ecoregions. The analysis further explored the impact of climatic variables on diameter at breast height (DBH) and basal area (BA) growth and yield. Among nine climatic attributes and their combinations, total precipitation and average growing season temperature significantly affected DBH and BA growth. However, yield was largely insensitive to precipitation, with only Sessile oak yield showing a temperature dependence. Beyond ecoregion and climate, the growth and yield of DBH and BA exhibited positive correlations with the calendar year, age, and previous growth/yield values. Notably, DBH and BA growth demonstrated a dependence on the preceding four to five years, whereas yield was significantly influenced only by the previous year. The observed influence of both the calendar year and previous years suggests a prolonged environmental memory in tree growth and yield responses. Full article
(This article belongs to the Topic Plant Responses to Environmental Stress)
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19 pages, 4300 KiB  
Article
Comparative Analysis of Bending and Rolling Shear Performance of Poplar and Hybrid Maple–Poplar Cross-Laminated Timber (CLT)
by Sumanta Das, Miroslav Gašparík, Anil Kumar Sethy, Peter Niemz, Manaswini Mahapatra, Rastislav Lagaňa, Nadežda Langová and Tomáš Kytka
J. Compos. Sci. 2025, 9(3), 134; https://doi.org/10.3390/jcs9030134 - 13 Mar 2025
Viewed by 1044
Abstract
Cross-laminated timber (CLT) is gaining popularity as a sustainable alternative to traditional building materials. However, the decline of natural vegetation and the growth of plantation hardwoods has led the researchers to consider alternatives. This study presents a comparative analysis of bending and rolling [...] Read more.
Cross-laminated timber (CLT) is gaining popularity as a sustainable alternative to traditional building materials. However, the decline of natural vegetation and the growth of plantation hardwoods has led the researchers to consider alternatives. This study presents a comparative analysis of bending and rolling shear performance of homogenous poplar (Populus nigra L.) CLT and hybrid CLT, with maple (Acer platanoides L.), in the outer layer and poplar in the core, compared to spruce (Picea abies (L.), H. Karst.) CLT. The CLT panels were prepared using one-component polyurethane (1C-PUR) and melamine adhesive (ME). Poplar CLT exhibited equal or better properties than spruce CLT. The outer maple layer in the hybrid CLT enhanced the global bending modulus (Emg) and bending strength (fm) by 74% and 37%, respectively, due to its higher modulus of elasticity better shear resistance by reducing the cross-layer stress concentrations and rolling shear failure. Additionally, both the adhesive types and wood species significantly influenced the fm, Emg, and rolling shear strength (fr) independently, while their interaction effect was found to be non-significant. The experimental bending stiffness was higher than the theoretical values. The shear analogy method provided the most accurate results for bending and shear strengths, while bending stiffness was best predicted by the modified gamma method, with minor variations. The finite-element models (FEMs) also produced results with a deviation of only 10%. Full article
(This article belongs to the Section Fiber Composites)
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18 pages, 11054 KiB  
Communication
Forest Soil Fungal Diversity in Stands of Norway Spruce (Picea abies (L.) Karst.) of Different Ages
by Juris Katrevičs, Krišs Bitenieks, Āris Jansons, Baiba Jansone and Dainis Edgars Ruņģis
Forests 2025, 16(3), 500; https://doi.org/10.3390/f16030500 - 12 Mar 2025
Cited by 1 | Viewed by 634 | Correction
Abstract
Fungal communities can be used as indicators of various environmental processes in forest ecosystems. The diversity of these communities is linked to aboveground plants and soil properties. We assessed fungal diversity at four Norway spruce sampling sites that were growing on fertile mineral [...] Read more.
Fungal communities can be used as indicators of various environmental processes in forest ecosystems. The diversity of these communities is linked to aboveground plants and soil properties. We assessed fungal diversity at four Norway spruce sampling sites that were growing on fertile mineral soils (Oxalidosa) in northwestern Latvia. Three sites were managed—a three-year-old clear-cut and fifty- and eighty-five-year-old stands; one site was unmanaged—a naturally regenerated site after wind damage in 1969. For metabarcoding, we used a fungal internal transcribed spacer (ITS2) and high throughput sequencing with the Ion Torrent platform. Our results showed high operational taxonomic unit richness in the samples, with notable variation in community composition between individual plots both within and among sites, with the highest being in managed, middle-aged stands and the lowest in unmanaged. Significant differences in the diversity of soil fungal communities were not detected between the sites. Redundancy analysis indicated that pH, soil organic matter, organic carbon, and nitrogen were the most important soil variables that explained the variation in fungal communities. The unmanaged stand differed notably by community composition. This study highlights the importance of monitoring forest soil environmental parameters and fungal communities to gain a more comprehensive assessment of forestry management regimes. Full article
(This article belongs to the Section Forest Biodiversity)
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12 pages, 1521 KiB  
Article
Carbon and Nitrogen Content and CO2 Efflux from Coarse Woody Debris of Norway Spruce, Black Alder, and Silver Birch
by Dovilė Čiuldienė, Egidijus Vigricas, Greta Galdikaitė, Vidas Stakėnas, Kęstutis Armolaitis and Iveta Varnagirytė-Kabašinskienė
Forests 2025, 16(2), 293; https://doi.org/10.3390/f16020293 - 8 Feb 2025
Viewed by 667
Abstract
Coarse woody debris (CWD) is an essential component in forest ecosystems, playing a significant role in enhancing biodiversity, soil formation, and nutrient cycling through decomposition processes. CWD also contributes to greenhouse gas fluxes, particularly through CO2 emissions. This study investigated the physical [...] Read more.
Coarse woody debris (CWD) is an essential component in forest ecosystems, playing a significant role in enhancing biodiversity, soil formation, and nutrient cycling through decomposition processes. CWD also contributes to greenhouse gas fluxes, particularly through CO2 emissions. This study investigated the physical and chemical properties of CWD and the CO2 effluxes from CWD of different decay classes. For this study, a range of CWD—from recently dead to highly decomposed wood—of native tree species such as silver birch (Betula pendula Roth), black alder (Alnus glutinosa (L.) Gaertn.), and Norway spruce (Picea abies (L.) H. Karst.) in hemiboreal forests were investigated. The findings showed that CWD properties significantly differed among tree species and CWD decay classes. Significant variations in wood density and total nitrogen (N) were observed in the early stages of CWD decay, with the highest values found for the deciduous tree species. The concentration of organic carbon (C) increased throughout the decomposition. The lowest CO2 efflux from CWD was found for spruce CWD from all decay classes and it was the highest for black alder and silver birch, especially for the 3rd and 4th decay classes. CO2 efflux was mainly influenced by the degree of decomposition, which was represented by the CWD decay class, followed by wood density and C content. Full article
(This article belongs to the Section Forest Ecology and Management)
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31 pages, 5135 KiB  
Article
Comparing Traditional Methods and Modern Statistical Techniques for Tree Height Prediction
by Jakob Hobiger, Ursula Laa and Sonja Vospernik
Forests 2025, 16(2), 271; https://doi.org/10.3390/f16020271 - 5 Feb 2025
Viewed by 803
Abstract
Forest mensuration is important to gain knowledge and information about forest stands. Because tree height often proves more difficult to measure than diameter, different statistical models are used for their estimation instead. In this paper, the data of 986 spruce trees (Picea [...] Read more.
Forest mensuration is important to gain knowledge and information about forest stands. Because tree height often proves more difficult to measure than diameter, different statistical models are used for their estimation instead. In this paper, the data of 986 spruce trees (Picea abies KARST. (L.)), measured in the federal states of Salzburg and Tyrol (Austria), were used to train and compare random forest with more traditional approaches such as linear and non-linear mixed models and a classical uniform height curve. For model comparison, RMSE, percent RMSE, percent bias, and bias are used. For further visualization of the differences, residual plots, partial dependence plots, and conditional dependence plots are shown. The results show that random forest (RMSE 2.23 m) can compete with more traditional methods, such as non-linear (RMSE 2.14 m) and linear (RMSE 2.24 m) mixed models or uniform height curves (RMSE 2.92 m), but is not able to outperform those methods, especially when it comes to extrapolation or prediction in areas where training data are sparse or not available. Furthermore, the results show that the incorporation of additional covariates can improve the prediction of certain models. Full article
(This article belongs to the Section Forest Inventory, Modeling and Remote Sensing)
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9 pages, 1061 KiB  
Article
Altitude and Stem Height Position as Determinants of the Hydrological Properties of Norway Spruce Bark
by Anna Ilek and Kelly Cristina Tonello
Forests 2025, 16(1), 156; https://doi.org/10.3390/f16010156 - 16 Jan 2025
Cited by 1 | Viewed by 769
Abstract
Tree bark plays a crucial role in the distribution of rainfall within forest ecosystems, particularly through its impact on stemflow. To gain a comprehensive understanding of how bark controls stemflow, it is essential to identify all factors affecting bark water storage capacity, as [...] Read more.
Tree bark plays a crucial role in the distribution of rainfall within forest ecosystems, particularly through its impact on stemflow. To gain a comprehensive understanding of how bark controls stemflow, it is essential to identify all factors affecting bark water storage capacity, as this determines the onset of stemflow during rainfall events. Our study analyzed how the position of bark on the stem and the altitude above sea level impact bulk density, water storage capacity, and the time required for bark saturation. We conducted research on Norway spruce bark collected at four altitudes: 400, 550, 700, and 1150 m asl. Our findings revealed that bark from the 400 m altitude had a bulk density that was approximately 24.5% greater than that from higher altitudes. Additionally, the water absorption time for bark from 1150 m was over 68% longer than that for bark from other altitudes. The longest absorption time (about 6.4 days) was observed in the bottom part of the trees, while the shortest (about 4.4 days) was in the top part of the trees. We also observed that the bark water storage capacity increased from the base to the top of the trees and with increasing altitudes. Specifically, the water storage capacity of bark taken from 400 m was approximately 33% lower than that from 1150 m. These findings highlight the significance of stem height position and altitude as key determinants of bark water storage capacity. Full article
(This article belongs to the Section Forest Hydrology)
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17 pages, 19045 KiB  
Article
Determination of the Critical Voltage for the Observation of Uncoated Wood Samples in Electron Microscopy
by Monika Sarvašová Kvietková, Ondřej Dvořák, Kryštof Kubista, Kristýna Těhníková, Chia-Feng Lin and Dennis Jones
Materials 2025, 18(2), 236; https://doi.org/10.3390/ma18020236 - 8 Jan 2025
Viewed by 701
Abstract
Electron microscopy (EM) is a key tool for studying the microstructure of wood; however, observing uncoated samples poses a challenge due to surface charging. This study aims to identify the critical voltage that allows for the effective observation of uncoated wood samples without [...] Read more.
Electron microscopy (EM) is a key tool for studying the microstructure of wood; however, observing uncoated samples poses a challenge due to surface charging. This study aims to identify the critical voltage that allows for the effective observation of uncoated wood samples without significant loading. As part of the experiment, samples of different wood species were tested, including Acacia (Robinia pseudoacacia L.), Oak (Quercus robur L.), Maple (Acer pseudoplatanus spp.), Ash (Fraxinus excelsior L.), Spruce (Picea abies (L.) Karst.), Thermowood (Thermal modifed Spruce), Garapa (Apuleia leiocarpa), Ipé (Handroanthus spp.), Merbau (Intsia bijuga), and Massaranduba (Manilkara spp.). Several methods were tested for surface preparation for SEM analysis, including the use of a circular saw, a hand milling machine, and a microtome. The results show that the optimal voltage for observing uncoated wood samples varied depending on the wood species. Regarding the selection of wood species and the results obtained, it was found that uncoated samples could be effectively observed. This finding suggests that practical observations can be accelerated and more cost-effective, as all wood species exhibited the required voltage range of 1 kV to 1.6 kV. Additionally, it was determined that using a secondary electron detector was optimal for such observations, as it provided a sufficiently strong signal even at relatively low voltages. Conversely, when using a backscattered electron detector, it was more beneficial to use coated samples to achieve a sufficient signal at higher voltages. This study brings new knowledge that will facilitate further research and applications of electron microscopy in the study of other wood species or wood-based materials. Full article
(This article belongs to the Special Issue Synthesis, Optimization, and Reuse of Sustainable Bio-Based Materials)
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18 pages, 1576 KiB  
Article
Response of Tree Seedlings to a Combined Treatment of Particulate Matter, Ground-Level Ozone, and Carbon Dioxide: Primary Effects
by Valentinas Černiauskas, Iveta Varnagirytė-Kabašinskienė, Ieva Čėsnienė, Emilis Armoška and Valda Araminienė
Plants 2025, 14(1), 6; https://doi.org/10.3390/plants14010006 - 24 Dec 2024
Viewed by 831
Abstract
Trees growing in urban areas face increasing stress from atmospheric pollutants, with limited attention given to the early responses of young seedlings. This study aimed to address the knowledge gap regarding the effects of simulated pollutant exposure, specifically particulate matter (PM), elevated ozone [...] Read more.
Trees growing in urban areas face increasing stress from atmospheric pollutants, with limited attention given to the early responses of young seedlings. This study aimed to address the knowledge gap regarding the effects of simulated pollutant exposure, specifically particulate matter (PM), elevated ozone (O3), and carbon dioxide (CO2) concentrations, on young seedlings of five tree species: Scots pine (Pinus sylvestris L.); Norway spruce (Picea abies (L.) H.Karst.); silver birch (Betula pendula Roth); small-leaved lime (Tilia cordata Mill.); and Norway maple (Acer platanoides L.). The main objectives of this paper were to evaluate the seedling stem growth response and the biochemical response of seedling foliage to pollutant exposure. Four treatments were performed on two- to three-year-old seedlings of the selected tree species: with PM (0.4 g per seedling) under combined O3 = 180 ppb + CO2 = 650 ppm; without PM under combined O3 = 180 ppb + CO2 = 650 ppm; with PM (0.4 g per seedling) under combined O3 < 40–45 ppb + CO2 < 400 ppm; and without PM under combined O3 < 40–45 ppb + CO2 < 400 ppm. Scots pine and Norway maple showed no changes in growth (stem height and diameter) and biochemical parameters (photosynthetic pigments, total polyphenol content (TPC), total flavonoids content (TFC), and total soluble sugars (TSS)), indicating a neutral response to the combined PM, O3, and CO2 treatment. The chlorophyll response to PM alone and in combination with elevated O3 and CO2 exposure varied, with silver birch increasing, Norway maple—neutral to increasing, Scots pine—neutral to decreasing, and Norway spruce and small-leaved lime—decreasing. The TPC indicated stress responses in Scots pine, small-leaved lime, and Norway maple under increased combined O3 and CO2 and in Norway spruce under single PM treatment. Hence, Scots pine and Norway maple seedlings showed greater resistance to increased PM under combined O3 and CO2 with minimal change in growth, while silver birch seedlings showed adaptation potential with increasing chlorophyll under simulated pollutant stress. Full article
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