Search Results (105)

The histological integrity of the intestine depends on the tight and orderly arrangement of epithelial cells within the intestinal villi. Nodal, a transforming growth factor-β (TGF-β) family member, has been reported to promote epithelial cell proliferation. Collagen not only establishes physical connections between adjacent cells but also serves as an anchoring platform for cell adhesion and regeneration processes. Therefore, a 21-day feeding trial was conducted using RNA interference to investigate the role of the Nodal gene in regulating intestinal collagen synthesis and histological structure integrity in juvenile A. japonicus fed diets containing graded levels of vitamin E (VE) (0, 200, and 400 mg/kg). The results showed that the addition of 200 mg/kg VE significantly improved the growth rate, immune enzyme activities and related gene expression, as well as intestinal villus morphology. Additionally, the addition of 200 mg/kg VE upregulated the expression of Nodal, which activated the expression of collagen synthesis-related genes and promoted collagen deposition in the intestines of A. japonicus. After Nodal gene knockdown, A. japonicus presented a decreased growth rate, damage to the intestinal histological structure, and impaired collagen synthesis, with the most notable findings observed in A. japonicus fed diets without VE addition. However, these detrimental effects were eliminated to some extent by the addition of 200 mg/kg VE. These findings indicate that VE improves immune function and intestinal histological structure in A. japonicus through a Nodal-dependent pathway. Full article

Preliminary tunnel surveys are essential for identifying geological hazards such as aquifers, faults, and karstic zones. While first-arrival tomography is effective for imaging shallow anomalies, traditional seismic sources face significant limitations in forested mountainous regions due to mobility, cost, and environmental impact. To address this, we deployed a seismic source delivered by an unmanned aerial vehicle (UAV) for a highway tunnel survey in Lijiang, China. The UAV system, paired with nodal geophones, enabled rapid, low-impact, and high-resolution data acquisition in rugged terrain. To enhance the weak far-offset refractions affected by near-surface attenuation, we applied supervirtual refraction interferometry (SVI), which significantly improved the signal-to-noise ratio and expanded the usable first-arrival dataset. The combined use of UAV excitation and SVI processing produced a high-precision P-wave velocity model through traveltime tomography, aligned well with borehole data. This model revealed the spatial distribution of weathered zones and bedrock interfaces, and allowed us to infer potential fracture zones. The results offer critical guidance for tunnel alignment and hazard mitigation in complex geological settings. Full article

(1) Background: Brain–computer interface (BCI) technology represents a cutting-edge field that integrates brain intelligence with machine intelligence. Unlike BCIs that rely on external stimuli, motor imagery-based BCIs (MI-BCIs) generate usable brain signals based on an individual’s imagination of specific motor actions. Due to the highly individualized nature of these signals, identifying individuals who are better suited for MI-BCI applications and improving its efficiency is critical. (2) Methods: This study collected four motor imagery tasks (left hand, right hand, foot, and tongue) from 50 healthy subjects and evaluated MI-BCI adaptability through classification accuracy. Functional networks were constructed using the weighted phase lag index (WPLI), and relevant graph theory parameters were calculated to explore the relationship between motor imagery adaptability and functional networks. (3) Results: Research has demonstrated a strong correlation between the network characteristics of tongue imagination and MI-BCI adaptability. Specifically, the nodal degree and characteristic path length in the right hemisphere were found to be significantly correlated with classification accuracy (p < 0.05). (4) Conclusions: The findings of this study offer new insights into the functional network mechanisms of motor imagery, suggesting that tongue imagination holds potential as a predictor of MI-BCI adaptability. Full article

Background: The Satyrinae subfamily represents a taxonomically critical group within Nymphalidae, characterized by its remarkable species diversity. Despite its evolutionary significance, the phylogenetic relationships among tribal and subtribal lineages remain poorly resolved. Although mitochondrial genomes have become crucial molecular markers in Lepidoptera phylogenetics, their potential remains underutilized in the systematics of Satyrinae. Notably, Amathusiini exhibits a particular paucity, with only two congeneric representatives having been comprehensively sequenced to date. Methods: We employed high-throughput sequencing to assemble the complete mitochondrial genomes of two Amathusiini species, Discophora sondaica and Aemona amathusia. Our study revealed novel evolutionary insights through comparative genomics, which encompassed all available Satyrinae mitochondrial genomes. Additionally, we conducted phylogenetic reconstruction using maximum likelihood and Bayesian inference approaches, utilizing the most extensive dataset to date. Results: The closed, circular mitochondrial genomes measure 15,333 bp for D. sondaica and 15,423 bp for A. amathusia, maintaining the ancestral lepidopteran architecture: 13 protein-coding genes (PCGs), 22 tRNAs, 2 rRNAs, and an AT-rich control region. Comparative analyses of 71 mitochondrial genomes revealed strong evolutionary conservation across multiple parameters: nucleotide composition (AT content range: 77.9–81.8%), codon usage bias (ENC = 30.83–37.55), tRNA secondary structures, and control region organization. All PCGs showed purifying selection signals (Ka/Ks < 1.0), with atp8 exhibiting the highest evolutionary rate (Ka/Ks = 0.277). Phylogenetic reconstructions yielded congruent tribal-level topologies with strong nodal support: ((Satyrini + Melanitini) + (Amathusiini + Elymniini) + Zetherini), confirming a sister relationship between Amathusiini and Elymniini. Within Satyrini, five subtribes formed monophyletic groups: Ypthimina, Erebiina, Maniolina, Satyrina, and Melanargiina, arranged as ((Ypthimina + (Erebiina + Maniolina)) + (Satyrina + Melanargiina)). Mycalesina, Lethina, and Parargina comprised a well-supported clade (BS = 100%; PP = 1.0), though internal relationships required further resolution due to Lethina’s polyphyly. Conclusions: This study provides novel insights into mitochondrial genomic evolution within the Satyrinae subfamily while elucidating the efficacy of mitogenomic data for resolving deep phylogenetic relationships within this ecologically significant subfamily. Our findings establish critical genome baselines for further systematic research and underscore essential pathways for refining subtribal-level taxonomy through integrative molecular approaches. Full article

This study proposes a full-cost electricity pricing model (M3) based on power flow tracing, addressing limitations in traditional nodal pricing and postage stamp methods. M3 dynamically allocates fixed transmission costs based on actual grid utilization, improving fairness, price signal accuracy, and congestion management. The model achieves fast convergence within 20 iterations across tested networks. Sensitivity analysis confirms that fuel costs and load variations significantly impact pricing, making M3 more adaptive and responsive. A regression-based forecasting model further enhances price predictability. The dual IEEE 118-bus case study validates M3’s feasibility in inter-provincial electricity markets, demonstrating its effectiveness for real-time pricing and investment planning. Full article

The asb5 gene, a member of the Asb protein subfamily characterized by six ankyrin repeat domains, is highly conserved and comprises two subtypes, asb5a and asb5b, in zebrafish. Our previous research has demonstrated that a deficiency of the asb5 gene significantly impairs early cardiac contractile function, highlighting its close relationship with heart development. Zebrafish asb5 expression was disrupted by both morpholino (MO) antisense oligomer-mediated knockdown and a CRISPR-Cas9 system. A high-throughput RNA-Seq analysis was used to analyze the possible molecular regulatory mechanism of asb5 gene deletion leading to left–right (L-R) asymmetry defects in the heart. Whole-mount in situ hybridization (WISH) was conducted to evaluate gene expression patterns of Nodal signaling components and the positions of heart organs. Heart looping was defective in zebrafish asb5 morphants. Rescue experiments in the asb5-deficiency group (inactivating both asb5a and asb5b) demonstrated that the injection of either asb5a-mRNA or asb5b-mRNA alone was insufficient to rectify the abnormal L-R asymmetry of the heart. In contrast, the simultaneous injection of both asb5a-mRNA and asb5b-mRNA successfully rescued the morphological phenotype. A high-throughput RNA-Seq analysis of embryos at 48 h post fertilization (hpf) revealed that numerous genes associated with L-R asymmetry exhibited expression imbalances in the asb5-deficiency group. WISH further confirmed that the expression of genes such as fli1a, acta1b, hand2, has2, prrx1a, notch1b, and foxa3 were upregulated, while the expression of mei2a and tal1 was downregulated. These results indicated that loss of the asb5 gene in zebrafish led to the disordered development of L-R asymmetry in the heart, resulting in an imbalance in the expression of genes associated with the regulation of L-R asymmetry. Subsequently, we examined the expression patterns of classical Nodal signaling pathway-related genes using WISH. The results showed that the midline barrier factor gene lefty1 was downregulated at early stages in the asb5-deficiency group, and the expression of spaw and lefty2, which are specific to the left lateral plate mesoderm (LPM), was disrupted. This study reveals that the two subtypes of the asb5 gene in zebrafish, asb5a and asb5b, interact and jointly regulate the establishment of early cardiac L-R asymmetry through the Nodal-spaw-lefty signaling pathway. Full article

With the advent of large-scale electronic transportation, the construction of electric vehicle charging stations (EVCSs) has increased. The stochastic characteristic of the charging power of EVCSs leads to a risk of destabilization of the DC distribution network when there is a high degree of power electronification. Current deterministic stability analysis methods are too complicated to allow for brief descriptions of the effect of probabilistic characteristics of EVCSs on stability. This paper develops a probabilistic small-signal stability analysis method. Firstly, the probabilistic information of the system is obtained by combining the s-domain nodal impedance matrix based on the point estimation method. Then, the probability function of stability is fitted using the Cornish–Fisher expansion method. Finally, a comparison experiment using Monte Carlo simulation demonstrates that this method performs well in balancing accuracy and computational efficiency. The effects of line parameters and system control parameters on stability are investigated in the framework of probabilistic stability. This will provide a probabilistic perspective on the design of more complex power systems in the future. Full article

The recognition of cytosolic nucleic acids is a critical step in the host immune response against danger signals, such as molecular patterns from pathogens or tissue damage. Nonetheless, over-reactivity to self-nucleic acids leads to the sustained production of type I interferon (IFN), mediated either by cGAS or RLR, contributing to the pathogenesis of certain autoimmune diseases, such as Aicardi–Goutières syndrome (AGS). Therefore, inhibiting excessive IFN production represents a potential therapeutic strategy for such autoimmune conditions. In this study, we discovered that petroselinic acid (PA), a natural compound isolated from Apiaceae family plants, effectively suppresses type I IFN production induced by cytosolic nucleic acids. Mechanistic investigations revealed that PA inhibits the phosphorylation of TBK1 and IRF3, which are key nodal proteins within the type I interferon pathway. Notably, molecular docking suggests potential binding between PA and cytosolic nucleic acid sensors, such as cGAS and RIG-I. Moreover, we found that PA effectively attenuates the expression of type I IFN and their downstream interferon-stimulated genes (ISGs) in models of AGS autoimmune disease characterized by excessive nucleic acid accumulation. Thus, our research identifies a natural compound that offers a promising strategy for treating autoimmune diseases resulting from aberrant self-nucleic acid recognition and the hyperactivation of type I interferon. Full article

Objectives: This study aimed to compare the imaging findings associated with the recurrence of HPV-positive and HPV-negative oropharyngeal squamous cell carcinoma (OPSCC). Methods: In total, 68 patients (51 men; mean age, 64.4 years; age range, 41–86 years; 48 HPV-positive patients and 20 HPV-negative patients) with histopathologically proven OPSCC who underwent CT, MRI, and 18F-FDG-PET/CT before treatment between October 2014 and July 2022 were enrolled in this study. The imaging findings were retrospectively evaluated and statistically compared. Results: HPV-positive OPSCC had a significantly lower recurrence rate compared with that of HPV-negative OPSCC (p < 0.01). Among HPV-positive OPSCCs, patients with recurrence were considerably older than those without recurrence (p < 0.05); however, the T and N categories did not differ between the two groups. Meanwhile, among HPV-negative OPSCCs, the T and N categories were associated with recurrence (p < 0.05). Furthermore, the attenuation on contrast-enhanced CT (p < 0.05) and signal intensity on contrast-enhanced T1-weighted images (p < 0.05) of nodal metastases were significantly lower in recurrence patients compared to those in nonrecurrence patients. Cystic change in nodal metastases in HPV-positive and HPV-negative OPSCCs were similar in patients with and without recurrence. Conclusions: The T and N categories were associated with recurrence in HPV-negative OPSCC but not in HPV-positive OPSCC. Prognostic factors differed significantly between HPV-positive and HPV-negative OPSCC. Full article

Electricity markets are transitioning to zonal and nodal pricing to maximize social welfare, improve price signals, and enhance congestion management. South Korea, traditionally reliant on uniform pricing, is evaluating this transition but lacks a detailed impact analysis. This study assessed the impacts of various zonal and nodal pricing schemes on power systems and provided policy implications for this transition. We (1) modeled the power system at various levels of granularity, obtaining a detailed 4579-node representation; (2) constructed a set of zonal and nodal pricing schemes reflecting changes in market-clearing models and pricing mechanisms; and (3) performed quantitative analyses through simulations for each scheme. Under the current system marginal price (SMP)-based uniform pricing, the schemes with the least market impact are SMP-based zonal pricing with two bidding zones and extended locational marginal pricing. These results can guide the development of an appropriate pricing transition pathway, although a market price reduction of 4.8–7.0% appears inevitable. Within the Korean electricity market, wherein a Transco is a monopoly retailer, we identified potential conflicts of interest for the Transco in zonal and nodal pricing. By focusing on South Korea, this study offers valuable insights into any electricity market considering the transition to zonal and nodal pricing. Full article

The accurate prediction of the aeroelastic behavior of turbomachinery for aircraft propulsion poses a difficult yet fundamental challenge, since modern aircraft engines tend to adopt increasingly slender blades to achieve a higher aerodynamic efficiency, incurring an increased aeroelastic interaction as a drawback. In the present work, we present a reduced order model for flutter prediction in axial compressors. The model exploits the aerodynamic influence coefficients technique with the adoption of a broadband frequency signal to compute the aerodynamic damping for multiple reduced frequencies using a single training simulation. The normalized aerodynamic work is computed for a single oscillation mode at three different vibration frequencies, comparing the outputs of aerodynamic input/output models trained with a chirp signal to those from single-frequency harmonic simulations. The results demonstrate the ability of the adopted model to accurately and efficiently reproduce the aerodynamic damping at multiple frequencies and arbitrary nodal diameters with a single simulation. Full article

Background/Objectives: Cutaneous squamous cell carcinoma (cSCC) is the second most common skin cancer, with a lifetime risk of 14–20% that is rising every year. Although prognosis for cSCC is generally good, certain high-risk features of cSCC portend increased rates of nodal and distant metastasis, recurrence, and disease-specific mortality. One such high-risk factor is perineural invasion (PNI), which is broadly defined as the invasion of cancer into and around nerves. Compared to other high-risk factors, PNI presence is associated with the highest risk for locoregional and distant metastasis. Still, the mechanisms underlying the pathogenesis of PNI remain poorly understood. Recent studies suggest the migration and invasion of tumors into nerves is a result of complex molecular crosstalk within the tumor-nerve microenvironment, wherein the milieu of signaling molecules simultaneously promote neuronal growth and tumor cell invasion. Methods: Understanding the molecular and cellular mechanisms that promote PNI will lead to future developments of targeted therapies that may improve locoregional control and survival. Results/Conclusions: In our article, we aim to provide a comprehensive review of recent findings about the pathogenesis of PNI, clinical implications of PNI-positive disease in cSCC, available treatment modalities, and potential future therapeutic targets. Full article

Background/Objectives: Cripto-1 (CR1) is a plurifunctional embryonic protein required for implantation and re-expressed in the adult during wound repair, inflammation, and tumorigenesis. CR1 and its predicted CR1 pseudogene product Cripto-3/CR3 are highly homologous proteins, and given this physical attribute, commercially available antibodies cannot discriminate between CR1 and CR3. Methods: A series of mouse monoclonal antibodies [MoAbs] were developed with a high-affinity binding that can differentiate human CR1/CR3 proteins and showed no measurable cross-reactivity. Results: Using these reagents, we confirm that CR3 is a bona fide translated protein found in human tumor tissue, cancer cell lysates, and in normal/cancer patient donor sera. We also reveal that CR1 and CR3 compete for binding to signal transduction protein Nodal, glucose-regulated protein 78Da (GRP78), and activin receptor-like kinase 4 (Alk4). Our discriminatory MoAbs provide new reagents to help clarify current CR1/CR3 protein expression vagaries in the Cripto field of study, challenging established CR1 conventions. In addition, our data validate CR3 involvement in human carcinogenesis and cell signaling pathways, with potential clinical relevance in determining cancer patient prognosis and disease severity. Full article

The decarbonization of power systems plays a great part in the carbon neutrality goal. Currently, researchers have explored reducing carbon in power systems in terms of the optimization of energy supply structure and operation strategies, but ignored the carbon reduction potential of users. To investigate the carbon reduction capability of users and further promote power system decarbonization through the active response of electricity loads, this paper proposes a carbon-reduction-oriented demand response (CRODR) technology based on generalized nodal carbon emission flow theory. First, the framework of the CRODR mechanism is established to provide an interaction baseline for users facing carbon reduction guiding signals. Secondly, the generalized nodal carbon emission flow theory is introduced to provide a calculation method for the guiding signals, considering dynamic electricity carbon emission factors with various spatiotemporal resolutions. Then, a matrix-based method is proposed to efficiently solve the carbon emission flow and obtain the guiding signals. On this basis, an optimal load-regulating model to help users meet their carbon reduction goals is built, and a carbon reduction benefit-evaluation method is proposed. Case studies on China’s national power system and a textile company verify that CRODR technology can realize efficient carbon reduction through load shifting while maintaining the total power consumption of users. The proposed CRODR technology is expected to provide a theoretical basis and guiding mechanism for promoting carbon reduction throughout the entire power system. Full article

Tunnels traditionally regarded as resilient to seismic events have recently garnered significant attention from engineers owing to a rise in incidents of seismic damage. In this paper, the reflection characteristics of the elastic plane wave incident on the free surface are analyzed, and the matrix analysis method SWIM (Seismic Wave Input Method) for the calculation of equivalent nodal loads with artificial truncated boundary conditions for seismic wave oblique incidence is established by using coordinate transformation technology, according to the displacement velocity and stress characteristics of a plane wave. The results show that the oblique incidence method is more effective in reflecting the traveling wave effect, and the “rotational effect” induced by oblique incidence must be considered for P wave and SV wave incidence, including the associated stress and deformation. This effect exhibits markedly distinct rotational phenomenon. In particular, the P wave incidence should be focused on the vault and the inverted arch due to the expansion wave. With the increase of the oblique incidence angle, the structural stress and deformation are rotated to a certain extent, and the values are significantly increased. Simultaneously, the shear action of the SV wave may result in “ovaling” of the tunnel structure, thereby facilitating damage to the arch shoulder and the sidewall components. As the oblique incidence angle, the potentially damaging effects of the “rotational effect” to the vault and the inverted arch, but the numerical value does not change significantly. In addition, in comparison to a circular cross-section, the low-frequency amplification of seismic waves in the surrounding rock and the difference of frequency response function in different parts of the lining are more pronounced. In particular, the dominant frequency characteristics are significant at P wave incidence and the seismic wave signal attenuation tends to be obvious with increasing incidence angle. In contrast, SV waves exhibit more uniform characteristics. Full article