Search Results (911)

Foliar respiration-to-photosynthesis ratio (R/A) is a key indicator of carbon balance and has been widely used to infer plant productivity and carbon sequestration potential. Yet, the extent to which R/A varies among genotypes within a species remains unclear. Chinese fir (Cunninghamia lanceolata (Lamb.) Hook.) is a dominant plantation conifer species in southern China. Here, we assessed genotypic variation in R/A and investigated its potential drivers in saplings of three Chinese fir genotypes (Gen3, Y020 and Y061) grown under uniform greenhouse conditions. R/A varied among genotypes, and the variation was driven mainly by differences in respiration, although photosynthesis also varied among genotypes. Compared to Gen3 and Y061, Y020 showed the highest R/A, indicating greater respiratory carbon loss relative to carbon gain. Across genotypes, respiration was positively associated with foliar nitrogen concentration (foliar N) and non-structural carbohydrate concentrations (NSC; including soluble sugars and starch), whereas photosynthesis was unrelated to foliar N and NSC. More importantly, R/A increased with NSC but not with foliar N. Overall, these results reveal substantial genotypic variation in R/A in Chinese fir saplings and suggest that NSC is an important factor associated with foliar carbon balance. Our findings have practical implications for selecting genotypes to enhance carbon sink capacity and stress resilience in plantation management. Full article

Developing sustainable strategies for natural resource management and conservation under shifting climatic scenarios is increasingly necessary due to exacerbated abiotic stresses, such as salinity. Under salt stress, several negative effects are observed in plants, particularly in leafy vegetables such as lettuce (Lactuca sativa L.). To mitigate the effects of saline stress from brackish water, several strategies have been adopted, including hydroponic cultivation. Therefore, this study aimed to determine the effects of variations in nutrient solution concentration and volume per lettuce plant cultivated in a nutrient film technique (NFT) hydroponic system using brackish water. The experiment was conducted using a randomized complete block design in a 2 × 2 × 2 factorial scheme, combining two levels of water electrical conductivity (ECw of 0.3 and 5.0 dS m⁻¹), two nutrient solution concentrations (NSC of 50 and 100%), and two nutrient solution volumes (NSV of 1 and 2 L plant⁻¹), with four replications. Growth, production, and water productivity variables were evaluated at 20 and 25 days following the imposition of treatments. The responses of the variables to saline stress varied according to the evaluation period (20 and 25 days), depending on the NSC and NSV levels. At the end of the 25-day cycle, it can be concluded that for lettuce cultivation using brackish water, the NSC can be reduced to 50% and provide an NSV of 2 L plant⁻¹. Under these growing conditions, leaf fresh matter production loss was approximately 40% lower than under cultivation without saline stress, which yielded 144.11 g plant⁻¹ under 100% NSC and an NSV of 2 L plant⁻¹. In contrast, water productivity of fresh matter was similar, at 78.68 and 76.55 g L⁻¹, respectively. Full article

Objectives: Alzheimer’s disease (AD) is associated with impaired adult hippocampal neurogenesis (AHN). This study aimed to establish an in vitro model of Aβ_1–42 oligomer-damaged neural stem cells (NSCs) and to employ the 5×FAD mouse model of AD in vivo, and to evaluate the therapeutic effects of brain-derived neurotrophic factor-loaded hyaluronic acid hydrogel (BDNF-HA gel) on AHN. Methods: In vitro, BDNF-HA gel was co-cultured with Aβ_1–42 oligomer-impaired NSC spheres and evaluate NSC proliferation, migration, and differentiation. In vivo, BDNF-HA gel was infused intracerebroventricularly into 5×FAD mice. Using BrdU labeling, immunofluorescence, anterograde transsynaptic viral tracing, and behavioral tests, we assessed the effects of BDNF-HA gel on adult neurogenesis, newborn neuron integration into memory circuits, and cognitive function. Results: In vitro, BDNF-HA gel attenuated Aβ_1–42-induced NSC apoptosis, restored proliferation and migration, promoted differentiation into neuroblasts, newborn neurons, and oligodendrocytes, and alleviated mitochondrial depolarization and loss of mitochondrial mass. In vivo, despite the absence of significant Aβ plaques reduction in 5×FAD mice, BDNF-HA gel markedly enhanced NSC proliferation and neurogenesis in the subventricular zone (SVZ) and subgranular zone (SGZ). Behavioral tests further revealed significant improvements in object recognition, spatial working memory, and spatial reference memory. Conclusions: BDNF-HA gel can effectively counteract the toxic microenvironment induced by Aβ oligomers, promoting NSC proliferation, migration, and differentiation into neurons. Without altering the Aβ burden, it significantly enhances adult neurogenesis and rescues cognitive deficits in AD mice. Full article

Current protocols for generating cortical interneurons from human pluripotent stem cells are hindered by slow differentiation kinetics and poor reproducibility across cell lines. Here, we present a defined small-molecule-based strategy that efficiently directs human-induced neural stem cells (hiNSCs) toward cortical GABAergic interneurons within 14–18 days, which is substantially faster than conventional methods. Short-term dual-SMAD and WNT inhibition rapidly commits hiNSCs to an interneuron progenitor fate, reaching transcriptional states equivalent to those obtained with prolonged protocols. Prolonged activation of Sonic Hedgehog (via SAG) further enhances lineage specification, markedly upregulating NKX2.1, FOXG1, GABA, somatostatin (SST), and parvalbumin (PV) expression, and enriching pathways associated with early functional maturation. Importantly, RNA-sequencing reveals that under identical induction conditions, hiNSCs differentiate more rapidly and homogeneously than human-induced pluripotent stem cells (hiPSCs), which exhibit broader, less lineage-focused transcriptional trajectories. This differentiation strategy is highly reproducible across four genetically distinct hiNSC lines, with minimal off-target populations. Functionally, hiNSC-derived cortical interneurons display robust migratory behavior, produce abundant GABA, and survive transplantation into the adult mouse hippocampus, where they extend processes and form synapse-like structures. These findings establish a rapid, scalable, and robust approach for generating human cortical interneurons, supporting their safety and integration potential as a foundation for future cell replacement strategies in neurological disorders. Full article

Background/Objectives: The sexuality of LGBTQIA+ individuals has been less studied than that of heterosexuals. This study aimed to examine, in cisgender men who have sex exclusively with men, the association of positive (PSC) and negative (NSC) sexual cognitions of dominance and submission with sexual arousal and erection in the contexts of solitary masturbation and sexual relationships. Methods: A sample of 253 men, aged 18 to 67 years old, completed an online survey assessing the frequency of PSC and NSC and their sexual functioning in solitary masturbation and sexual relationships. Mixed linear models were used to analyze the association of PSC and NSC of dominance and submission with sexual arousal and erection in both contexts. Results: The results revealed, first, a lack of association between PSC and arousal/erection, except for a negative association with a submission PSC (“being sexually victimized”). Regarding NSC, on one hand, a positive relationship of a specific dominance cognition (“whipping or spanking someone”) with arousal and erection was found. On the other hand, a negative association of a specific submission cognition (“being whipped or spanked”) with arousal was found. Furthermore, the interaction of this NSC of dominance with the context was significant. Conclusions: The results obtained in this study highlight the complexity of the relationship between positive (PSC) and negative (NSC) sexual cognitions of dominance and submission with sexual arousal and erection in cisgender men who have sex exclusively with other men. Full article

Steel–concrete composite beams are widely used in bridge infrastructure but are vulnerable to deterioration due to uniform and pitting corrosion, particularly at the lower flange. This study investigates the flexural behavior of corroded steel–normal strength concrete (NSC) composite beams and evaluates rehabilitation using ultra-high-performance concrete (UHPC) slab replacement, with and without additional steel plate strengthening. A comprehensive finite element analysis was conducted considering three beam spans (5, 7, and 9 m), two corrosion types, and three corrosion levels. The results indicate that both corrosion types significantly reduce flexural capacity due to cross-sectional loss, with pitting corrosion causing greater strength reduction than uniform corrosion at the same weight loss because of stress concentration effects. Replacing the NSC slab with a UHPC slab effectively restores and often enhances load-carrying capacity beyond that of intact beams while reducing dead load, demonstrating the superiority of the proposed rehabilitation approach. The combined use of UHPC slab replacement and welded steel plate strengthening provides the greatest improvement, revealing a strong synergistic effect. A case study of a corroded steel bridge in Pennsylvania confirms the practical applicability of the method, showing that UHPC-based rehabilitation increases the load rating from below unity to above unity. These findings highlight UHPC as an efficient and sustainable solution for extending the service life of aging steel bridges. Full article

Neuritogenesis is essential for neuronal development and circuit formation. Although cAMP signaling downstream of Gs-coupled receptors is considered pro-neuritogenic, activation of these Gs-coupled receptors can produce divergent cellular outcomes. We previously showed that prostaglandin E₂ (PGE₂) induces neurite outgrowth in NSC-34 motor neuron-like cells predominantly through Gs-coupled E-prostanoid receptor 2 (EP2) signaling. The I-prostanoid receptor (IP) is also Gs-coupled, but whether its ligand PGI₂ elicits neuritogenesis remains unclear. Here, we compare the neuritogenic and signaling responses to PGE₂ and PGI₂ in NSC-34 cells. PGE₂ and the EP2 agonist butaprost increased the proportion of neurite-bearing cells, whereas PGI₂ and the IP agonist beraprost had no effect. PGI₂ and PGE₂ induced comparable cAMP accumulation and protein kinase A substrate phosphorylation, and elicited peak cAMP response element binding protein (CREB) phosphorylation at 1 h. However, only PGE₂ maintained significant CREB phosphorylation at 3–6 h. RNA sequencing at 4 h revealed broadly concordant transcriptional responses, while direct comparison identified Fst as the only gene expressed at higher levels under PGE₂ than under PGI₂. These findings suggest that the temporal profile of CREB phosphorylation, rather than the magnitude of early cAMP-PKA signaling, may be associated with differences in neuritogenic outcomes of Gs-coupled prostanoid signaling. Full article

Simulations of intermediate-mass black holes (IMBHs) in dwarf galaxies within 10 Mpc that host bright nuclear star clusters (NSCs), prime candidates for IMBH formation, using the High Angular Resolution Monolithic Optical and Near-infrared Integral (HARMONI) field spectrograph on the Extremely Large Telescope, probe black hole formation in the early universe. Our approach combines observed surface-brightness profiles from the Hubble Space Telescope (HST), synthetic stellar population spectra, and Jeans Anisotropic Modeling (JAM) for stellar dynamics. Mock HARMONI observations were generated with the HSIM simulator and analyzed in a Bayesian framework to infer IMBH masses down to 0.5% of the NSC mass. In this work, we extend these simulations by constructing improved stellar mass models using SimCADO to simulate imaging with the Multi-AO Imaging Camera for Deep Observations (MICADO). The MICADO data are jointly analyzed with HARMONI kinematics via JAM to reassess IMBH masses and uncertainties. This combined framework enables us to examine how variations in the NSC inner surface-brightness slope influence IMBH mass estimates, providing tighter constraints on low-mass black holes and advancing models for IMBH detection in NSCs. Full article

Fertility traits are core functional traits in dairy cattle, yet their low heritability makes traditional phenotypic selection inefficient. Weighted single-step GWAS (WssGWAS) improves detection power by integrating pedigree, phenotype, and genotype data. This study aimed to estimate genetic parameters and identify genomic regions associated with eight fertility traits in Chinese Holstein cattle using pedigree, phenotype, and genotype data. Genetic parameters were estimated via pedigree-based BLUP (PBLUP) and ssGBLUP, and a WssGWAS was performed to detect QTL. A total of 23,771 cattle were genotyped using liquid-phase chips and 150 K chips; after imputation and quality control, 13,690 individuals and 103,262 SNPs were retained. The eight traits comprised heifer traits (AFS, AFC, NS_H, and IFL_H) and cow traits (ICF, CI, NS_C, and IFL_C). The findings indicate that while most fertility traits exhibit low heritability (ranging from 0.024 to 0.085), AFS displays high heritability (0.390). WssGWAS identified 162 QTL intervals and 645 candidate genes, including OVOS2, PMEPA1, TEPP, and the RAG family, which are closely associated with reproductive function. These findings provide a foundation for future research into the genetic mechanisms of fertility and support improved genomic predictions in Chinese Holstein cattle. Full article

Pure electric construction machinery (PECM) is gradually becoming the mainstream choice for industrial construction. This paper presents a new configuration of an integrated charger for PECM. The proposed configuration employs a nine-switch-converter (NSC) that can achieve charging and traction functions for the target application. In charging mode, the motor is reused as a filter inductor and the NSC is reused as a conventional three-phase PWM rectifier. Data-driven adaptive predictive control (DAPC) based on recursive least squares (RLS) is proposed to cope with the motor’s saturation problem in charging mode. This control has the advantages of excellent robustness and fast dynamic response. Although the initial parameters are derived from the system model in the first sampling cycle, the controller subsequently relies entirely on online identification, which significantly reduces the sensitivity to parameter accuracy and eliminates the need for manual tuning of controller gains. In propulsion mode, the NSC enables independent operation of the two motors. The proposed configuration improves the utilization of devices and motors, which greatly reduces the weight, volume, and cost of the charger. Finally, an experimental platform was built to verify the feasibility and validity of the proposed topology and control algorithm. Full article

Omega-6 polyunsaturated fats (ω-6 PUFAs) are vital for many physiological functions, but their impact on cardiovascular disease (CVD) risk is controversial. Eryptosis alters blood viscosity by providing a procoagulant surface and leads to anemia, which is a recognized risk factor for CVD. This study examines the toxic mechanisms of adrenic acid (ADR), an ω-6 PUFA enriched in inflammatory and oxidative conditions, in red blood cells (RBCs). Purified RBCs were prepared from healthy volunteers and treated with 10–100 μM of ADR for 24 h at 37 °C under various physiological conditions. Eryptotic markers were studied through flow cytometry including Ca²⁺ (Fluo4/AM), loss of volume (forward scatter), phosphatidylserine (PS) exposure (annexin-V-FITC), and oxidative stress (H₂DCFDA). Moreover, hemolytic markers were measured by colorimetric methods, whereas cellular morphology was visualized using a scanning electron microscope. ADR led to significant Ca²⁺ elevation, cell shrinkage and schistocyte formation, PS externalization, hemolysis, and oxidative stress. While guanosine, heparin, and NSC 23766 prevented eryptosis and hemolysis, melatonin, ATP, adenine, and L-NAME only prevented eryptosis. Conversely, mannitol and urea exacerbated eryptosis, whereas caffeine, mannitol, and urea under Ca²⁺ deprivation and membrane potential dissipation aggravated hemolysis. ADR induces erythrocyte membrane injury and eryptosis through Ca²⁺ elevation, oxidative stress, and metabolic exhaustion subject to inhibition by the Rac1 GTPase/NOS/COX pathway. Altogether, these findings present a novel mechanistic link between lipid dysregulation and RBC dysfunction which may improve dietary strategies to prevent and manage CVD. Full article

Coronavirus disease 2019 continues to pose global health challenges, with the pandemic significantly burdening several economies, healthcare systems, and the social lives of individuals. Furthermore, new cases continue to be reported, underscoring the need for therapeutic strategies targeting conserved regions and host–virus interactions. Building on earlier virtual screening for small molecules, all-atom molecular dynamics simulations and binding-free-energy calculations were performed to elucidate how the two previously identified small molecules (NSC36398 and NSC281245) may affect the dynamic behaviour of the interaction between heat shock 70 kDa protein 8 (HSPA8) and the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike glycoprotein. Post-MD analyses refined prior docking predictions, where NSC281245 was found to bind tightly to the complex with limited perturbations at the HSPA8-spike protein interaction surface, whereas NSC36398 appeared to induce allosteric-like domain-level destabilisation effects while maintaining stable polar contacts with the protein. Our findings demonstrate the potential of NSC36398 as a promising modulator for disrupting the HSPA8-spike protein complex, which may serve as a structural lead for designing next-generation inhibitors of host–virus interactions. Full article

Adult neural stem cells (NSCs) maintain lifelong neurogenesis, a fundamental process for neuroplasticity, memory and brain homeostasis. Despite decades of research, translating basic NSC biology into effective clinical therapies remains a central challenge. Here we present a narrative review that provides a comprehensive update on the current landscape of adult NSC research, associating molecular mechanisms with the emerging translational technologies. First, we analyze the biological features and neurogenic sequences within canonical niches such as the subventricular lateral zone and the subgranular zone, emphasizing phylogenetic and migratory differences between rodent models and humans. Second, we integrate these mechanisms with the influence of environmental and pathological modulators, describing how aging, metabolic changes, chronic stress and neuroinflammation disrupt NSC quiescence and lineage progression. Finally, we highlight recent technological advances driving the field toward clinical applications. By examining current NSC isolation strategies, induced pluripotent stem cell modeling, direct somatic reprogramming and the use of CRISPR-Cas9-based gene-editing therapies, this review delineates the pathways to overcome existing methodological limitations. Ultimately, we provide an integrated context that connects the modulation of the neurogenic niches with advanced in vitro technologies, offering new perspectives for regenerative medicine and the treatment of neurological disorders. Full article

Although transplantation of induced oligodendrocyte progenitor cells (iOPCs) is a promising strategy for white matter injury, the therapeutic efficacy of in vitro-generated iOPCs remains limited due to insufficient differentiation potential. Here, we aimed to identify key transcription factors and small-molecule drugs to optimize iOPC quality. Through transcriptome sequencing and bioinformatics analysis, we identified the transcription factor SOX10, which is differentially expressed between endogenous fetal OPCs and exogenous iOPCs. We established lentivirus-mediated SOX10 overexpression in neural stem cells (NSCs) before iOPC induction and performed cellular assays and multi-omics analysis. Early SOX10 overexpression reduced cell migration but promoted maturation into oligodendrocytes and suppressed astrocyte differentiation. Multi-omics analyses revealed that SOX10 overexpression is associated with the extensive redistribution of SOX10 chromatin binding and enrichment of regulatory programs linked to oligodendroglial differentiation, including the activation of the key signaling downstream transcription factors JUN/FOS. Moreover, TSA, Dabrafenib, and Fedratinib effectively upregulated SOX10 and improved iOPC differentiation. This study identifies SOX10 as a core upstream regulator governing the fate of iOPCs, providing a potential strategy for optimizing iOPC induction for future investigation of white matter injury therapy. Full article

Background: The elderly are vulnerable to chronic diseases and altered lipid metabolism, leading to poor outcomes, including mortality. We investigated the association between Korean Medicine (KM) utilization, blood lipid levels, and health outcomes using the National Health Insurance Service Sample Cohort (NHIS-NSC) database. Methods: This retrospective cohort study included elderly participants who underwent health examinations (2009–2010). Participants were divided into KM and non-KM groups and matched 1:1 using propensity score matching (PSM) for age, sex, income, and comorbidities. Primary outcomes were mortality and disease diagnosis; secondary outcomes included medical spending and utilization. Results: After PSM, 13,044 subjects were analyzed. KM utilization was associated with a significantly lower risk of all-cause mortality (HR 0.93; 95% CI 0.87–1.00; p = 0.048). However, the hypolipidemia subgroup showed no significant differences in all-cause mortality and medical expenses compared to other lipid status subgroups. While the KM group showed a higher incidence of disease diagnosis (HR 1.09; 95% CI 1.04–1.14; p < 0.001), this may reflect increased healthcare engagement and proactive health-seeking behavior. Subgroup analysis revealed that statin users in the KM group had a significantly reduced mortality risk (HR 0.91; 95% CI 0.84–0.99; p = 0.022). Medical expenses and utilization were higher in the KM group. Being underweight or aged over 85 was associated with higher mortality. Conclusions: KM utilization is associated with reduced all-cause mortality after propensity score matching, particularly among statin users. Although KM users had a higher cumulative incidence of disease diagnosis, this potentially reflects increased diagnostic opportunities from prolonged survival. Hypolipidemia, underweight, and late-elderly status remain significant risk factors associated with frailty. KM may support improved survival in the elderly, warranting further prospective studies. Full article