Search Results (1,067)

This article studies the application of deep neural networks for automatic building detection in aerial RGB images. Special focus is put on accuracy robustness in both well-structured and poorly planned urban scenarios, which pose significant challenges due to occlusions, irregular building layouts, and limited contextual cues. The applied methodology considers several CNNs using only RBG images as input, and both validation and transfer capabilities are studied. U-Net-based models achieve the highest single-model accuracy, with an Intersection over Union ($IoU$) of 0.9101. A soft-voting ensemble of the best U-Net models further increases performance, reaching a best ensemble $IoU$ of 0.9665, improving over state-of-the-art building detection methods on standard benchmarks. The approach demonstrates strong generalization using only RGB imagery, supporting scalable, low-cost applications in urban planning and geospatial analysis. Full article

Oxidative stress (OS) has gained substantial relevance due to its involvement in the pathogenesis of numerous systemic diseases. It is characterized by an imbalance between the production of reactive oxygen species (ROS) and the capacity of endogenous antioxidant systems to neutralize them. Various factors, including trauma, immunological alterations, and psychological stress, may contribute to this condition. The aim of this narrative review was to analyze OS markers and total antioxidant capacity (TAC) in asymptomatic and pericoronitis-associated impacted mandibular third molars (ITMs). This review examines the relationship between OS and impacted ITMs, highlighting the importance of timely clinical management to prevent chronic tissue damage. Current evidence indicates that OS biomarkers such as myeloperoxidase (MPO), malondialdehyde (MDA), uric acid (UA), and nitric oxide (NO) are elevated in patients with ITMs, including those classified as asymptomatic, and that a reduction in total antioxidant capacity (TAC) has been observed. The surgical removal of ITMs can effectively reduce OS levels. Following the procedure, oxidative markers typically return to normal within a relatively short period of time, and there is often a significant improvement in TAC. Full article

The intensive use of chemical fertilizers in soybean (Glycine max) cultivation has caused significant environmental degradation, underscoring the urgent need for sustainable alternatives. In Argentina, Bradyrhizobium japonicum E109 is widely employed as a liquid bioinoculant, yet its efficiency is limited by loss of viability during storage. This study investigated the physiological and biophysical mechanisms underlying membrane adaptation of B. japonicum E109 under storage stress and evaluated lipid supplementation as a stabilization strategy. During six months of liquid storage at 28 °C, bacterial viability (Log CFU mL⁻¹) declined from 10.0 to 7.7, accompanied by morphological collapse and a 29% reduction in membrane fluorescence polarization, indicating increased fluidity. Fatty acid analysis revealed a drastic decrease of unsaturated 18:1 (from 80% to 40%) and a 300–400% increase in saturated 18:0, reducing the U/S ratio from 4 to 1. Spectral phasor analysis confirmed a shift in the lipid microenvironment from an ordered to a disordered state. Supplementation with 400 µM of stearic acid (18:0) restored membrane rigidity, lowered the U/S ratio to 1.5, and improved thermal tolerance. After one month of storage, 18:0-treated cultures maintained 8.0 Log CFU mL⁻¹ and preserved viability after exposure to 37 °C, whereas controls dropped to 3.8 Log CFU mL⁻¹. These results identify lipid remodeling as a key determinant of B. japonicum stability and demonstrate that exogenous 18:0 supplementation mimics natural adaptation, preventing membrane fluidization and enhancing inoculant shelf-life. This lipid-biophysical approach provides a rational framework for developing next generation, more resilient rhizobia formulations for sustainable agriculture. Full article

This study illustrates the application of Acceptance and Commitment Therapy (ACT) for a 12-year-old boy with separation anxiety and his mother. Over 23 sessions, ACT strategies promoted psychological flexibility, values-based parenting, and adaptive behaviors. The intervention reduced the child’s experiential avoidance, anxiety, and depressive symptoms, while increasing value-oriented actions, while the mother showed improved psychological flexibility and life satisfaction. The results were sustained at a three-month follow-up. This case study highlights the potential of ACT in treating childhood separation anxiety by simultaneously involving parents, demonstrating its feasibility and efficacy. The findings provide guidance for adapting ACT for families and child populations. Full article

Climate change is widely recognized as a significant contributor to both wildfire initiation and spread, conditions such as high temperatures and prolonged droughts facilitating the rapid ignition and propagation of fires. As a result, extreme weather events can trigger fires through lightning strikes with increases in frequency and severity. Despite this, we argue that it is important to distinguish and clarify the concepts of fire occurrence and fire spread, as these phenomena are not directly synonymous in the field of fire ecology. This review examined the published literature to determine if climate factors contribute to fire occurrence and/or spread, and evaluated how well the concepts are used when drawing connections between fire occurrence and fire spread related to climate variables. Using the PRISMA bibliographic analysis methodology, 70 scientific articles were analyzed, including reviews and research papers in the last 5 years. According to the analysis, most publications dealing with fire occurrence, fire spread, and climate change come from the northern hemisphere, specifically from the United States, China, Europe, and Oceania with South America appearing to be significantly underrepresented (less than 10% of published articles). Additionally, despite climatic variables being the most prevalent factors in predictive models, only 38% of the studies analyzed simultaneously integrated climatic, topographic, vegetational, and anthropogenic factors when assessing wildfires. Furthermore, of the 47 studies that explicitly addressed occurrence and spread, 66 percent used the term “occurrence” in line with its definition cited by the authors, that is, referring specifically to ignition. In contrast, 27 percent employed the term in a broader sense that did not explicitly denote the moment a fire starts, often incorporating aspects such as the predisposition of fuels to burn. The remaining 73 percent focused exclusively on “spread.” Hence, caution is advised when making generalizations as climate impact on wildfires can be overestimated in predictive models when conceptual ambiguity is present. Our results showed that, although climate change can amplify conditions for fire spread and contribute to the occurrence of fire, anthropogenic factors remain the most significant factor related to the onset of fires on a global scale, above climatic factors. Full article

Background: Fasciola hepatica (F. hepatica) infection reduces antibody avidity to foot-and-mouth disease virus (FMDV) vaccination in cattle despite preserved total antibody levels. However, its effect on vaccine-induced immunity in water buffaloes (Bubalus bubalis), which contribute to FMDV maintenance in endemic settings, has not been investigated. Objectives: To evaluate the effect of natural F. hepatica infection on the magnitude and functional quality of the FMDV–specific antibody response in buffaloes under field conditions. Methods: Two buffalo herds (n = 50 each) were classified by infection status using coproparasitological analysis and serology. All animals were vaccinated within the national foot-and-mouth disease control programme, with the last dose administered 264 days before sampling. Serum neutralising titres, total antibodies by liquid-phase blocking ELISA, IgG levels, and IgG avidity to the A24/Cruzeiro vaccine strain were determined. Results: F. hepatica-infected buffaloes exhibited consistent decreases across all vaccine-induced antibody parameters. Neutralising titres were reduced approximately two-fold, IgG avidity by about 38 percent, IgG levels by about 36 percent, and liquid-phase blocking ELISA titres by about 1.6-fold compared with non-infected animals. Conclusions: This study provides the first field evidence that fasciolosis is associated with changes in the magnitude and quality of vaccine-induced humoral responses following FMDV vaccination in water buffaloes, indicating that F. hepatica infection may influence the interpretation of post-vaccination serological monitoring in this species under endemic field conditions. Full article

Minthostachys verticillata (Griseb.) Epling, commonly known as peperina, is an aromatic species endemic to Argentina and traditionally used for gastrointestinal ailments. Despite its extensive folkloric use and inclusion in the Argentine Pharmacopoeia, its aqueous extract (the most commonly consumed preparation) has been described in terms of major phytochemical groups, and, currently, no studies have investigated its effects on key intestinal epithelial mechanisms. This plant is also employed in the production of beverages and herbal blends, and its massive consumption highlights the importance of its scientific study. Here, the aqueous extract of M. verticillata was characterized by liquid chromatography–tandem mass spectrometry, leading to the identification of fourteen polyphenolic compounds. In intestinal cell models, the extract displayed high IC₅₀ values, supporting its safety, and exhibited concentration-dependent bioactivity. In HT-29 cells, it modulated NF-κB activation induced by TNF-α and reduced LPS-stimulated IL-8 production. Pretreatment of Caco-2 monolayers prevented the decrease in transepithelial electrical resistance, increased FITC–dextran permeability, and nitric oxide production triggered by an inflammatory cocktail. Additionally, the extract inhibited HT-29 cell migration. These results demonstrate that M. verticillata aqueous extract exerts anti-inflammatory, barrier-protective, and anti-migratory effects in vitro, providing novel insights into how its polyphenolic composition may underlie these biological activities, supporting its traditional use and potential applications in intestinal health. Full article

Vaccination remains a cornerstone of livestock disease control, yet its effectiveness under field conditions is often compromised by concurrent infections, particularly parasitic helminths. This review explores how infections shape vaccine-induced immunity in cattle, emphasizing the immunoregulatory mechanisms by which helminths interfere with protective responses. Chronic infections with Fasciola hepatica and Ostertagia ostertagi induce Th2-biased and regulatory immune environments that suppress antigen presentation, cytokine production, and memory formation and maintenance, leading to reduced vaccine efficacy. Evidence from experimental and field studies is scarce and constitutes a gap in our knowledge on how vaccines work in the field. Available data indicate that infection timing, intensity, and chronicity critically determine the extent of vaccine interference. The review highlights diagnostic approaches that can support targeted deworming before vaccination and proposes integrated management strategies combining parasite control, immunization, and nutritional optimization. Such approaches can mitigate helminth-driven immune suppression, enhance herd protection, and reduce dependence on anthelmintics. However, the impact of helminth infections on vaccine efficacy in cattle should be further assessed in the field. Understanding parasite–vaccine interactions is essential to refine vaccination programs, guide the development of next-generation vaccines, and promote sustainable livestock health in parasite-endemic areas. Full article

This paper introduces GECO, a real-time, computer vision-assisted gesture controller for IoT-based smart home systems. The platform uses a markerless MediaPipe interface that combines gesture-driven navigation and command execution, enabling intuitive control of multiple domestic devices. The system integrates binary and analog gestures, such as continuous light dimming based on thumb–index angles, while operating on-device through a private MQTT network. Technical evaluations across multiple Android devices have demonstrated ultra-low latency times (<50 ms), enabling real-time responsiveness. A user experience study with seventeen participants reported high intuitiveness (9.5/10), gesture accuracy (9.2/10), and perceived inclusivity, mainly for individuals with speech impairments and low technological literacy. These results position GECO as a lightweight, accessible, and privacy-preserving interaction framework, advancing the integration of artificial intelligence and IoT within smart home environments. Full article

Bacteria-based cancer immunotherapies are regaining attention due to recent advances in understanding the mechanisms underlying their efficacy, making them promising tools for cancer treatment. Among these, Salmonella stands out as one of the most extensively studied microorganisms in this field. Its ability to directly induce tumor cell death while stimulating the immune system offers unique therapeutic advantages, as cell death within an inflammatory environment may enhance the release of tumor antigens and promote effective antitumor immune responses. Although multiple studies have addressed Salmonella-induced cell death, the nomenclature and classification of death modalities are often inconsistent—either because earlier reports predate the formalization of certain death pathways, or due to overlapping criteria between different types of cell death. This review aims to comprehensively analyze the available evidence on Salmonella-induced apoptosis, pyroptosis and autophagy, as well as other less characterized death modalities. Given that most mechanistics evidence on Salmonella-induced cell death has been generated in myeloid cells, we primarily focus on the myeloid compartment while integrating available observations from tumor cells and other immune populations when relevant, organizing the existing data under current definitions and concepts, and highlighting the challenges of manipulating these pathways to optimize bacterial-based immunotherapies. Full article

The decarbonization of transport demands efficient, low-carbon alternatives to conventional fuels, particularly in regions where full electrification remains constrained. This study investigates the retrofitting of a 1.3 L Geely MR479Q spark-ignition engine for hydrogen operation, combining experimental measurements and one-dimensional numerical simulations in GT-SUITE. The baseline gasoline model was experimentally validated in 12 operating conditions and extended to the full map. In addition, the fuel was changed in the numerical model, and evaluations of hydrogen combustion through predictive sub-models considering mixture formation and pressure-rise limits were performed. Results show that the hydrogen engine operates stably within a wide air–fuel ratio window (λ = 1.0–2.7), with brake thermal efficiencies peaking at approximately 29%, surpassing gasoline operation by up to 5% in the mid-load range. However, port fuel injections cause a reduction in volumetric efficiency and maximum power output due to air displacement, a limitation that could be mitigated by adopting direct injection. A practical hydrogen conversion kit was defined—including injectors, cold-type spark plugs, electronic throttle, and programmable ECU—and the operational cost was analyzed. Economic parity with gasoline is achieved when hydrogen prices fall below ~6 USD kg⁻¹, aligning with near-term green-hydrogen projections. Overall, the results confirm that predictive numerical calibration can effectively support retrofit design, enabling efficient, low-emission combustion systems for sustainable transport transitions. Full article

Poultry serve as natural reservoirs for Salmonella spp., and the consumption of Salmonella-contaminated poultry products remains a leading cause of infection in both developed and developing countries. The irrational use of antimicrobials in the poultry industry has contributed to the global emergence of multidrug-resistant (MDR) Salmonella strains. This study aimed to evaluate whether Salmonella enterica serovar Typhimurium (S. typhimurium) outer membrane vesicles (OMVs) could serve as a broad-spectrum immunogen, providing protection against the most prevalent MDR Salmonella serovars. The immunogenicity and protective efficacy of S. typhimurium OMVs were assessed in both specific-pathogen-free (SPF) mice and chickens challenged with S. typhimurium infection. In addition, the cross-protective efficacy of OMVs against infections with heterologous serovars, specifically S. montevideo and S. albany, was also evaluated in chickens. Our results demonstrated that S. typhimurium OMVs elicited a robust humoral immune response and conferred significant immune protection in both mice and chickens. Following challenges with MDR S. montevideo and S. albany strains, immunized birds exhibited reduced levels of fecal shedding and liver invasion. Furthermore, in vitro studies revealed that S. typhimurium OMVs also possess cross-immunogenicity against MDR S. Enteritidis and S. gallinarum. In conclusion, S. typhimurium OMVs provide cross-protection against heterologous MDR Salmonella infections both in vitro and in vivo. Our study suggests that OMVs derived from S. typhimurium strains hold promise as novel subunit vaccine candidates for preventing MDR heterologous serovar infections in animals, offering a potential solution to the growing challenge of antimicrobial resistance in the poultry industry. Full article

Canavalia ensiformis (L.) DC. (Fabaceae) is used in Cuba in soils dedicated to coffee cultivation, contributing to soil nutrition and crop productivity. However, no rhizobial isolates are currently available for inoculating this legume in Chromic Eutric Cambisol soils. The aim of this study was to select rhizobial strains that promote the growth of C. ensiformis in Chromic Eutric Cambisol soils. Nodules were collected from C. ensiformis plants, surface-sterilized, and macerated to isolate potential rhizobia. The isolates were characterized based on cultural, morphological, and biochemical traits, and their symbiotic effectiveness was evaluated through in vitro inoculation assays in Macroptilium atropurpureum (siratro) plants. Inoculation trials were conducted under semi-controlled conditions and in the field between coffee rows. The number and dry weight of effective nodules, number of trifoliate leaves, and shoot dry biomass were measured. Nine bacterial isolates were obtained, grouped into four morphotypes, and assigned as possible members of the families Phyllobacteriaceae, Methylobacteriaceae, or Nitrobacteraceae. Under semi-controlled conditions, inoculation with three isolates increased the number of nodules (by 56–80%), the number of trifoliate leaves (by 20–45%), and shoot biomass (by 10–40%) compared to the non-inoculated treatment. Additionally, one of the isolates increased nodule dry weight by 27%. In the field between coffee row, increases were also observed in the number of trifoliate leaves (by 18–26%) and shoot biomass (by 15–24%). This study supports the selection of efficient rhizobia adapted to the edaphoclimatic conditions of Cuban coffee agroecosystems. Full article

Integrating perennial grain crops into agricultural systems can become a key milestone for increasing the provision of ecosystem services of food production systems. Intermediate wheatgrass is a novel perennial grain and forage crop that is undergoing domestication. Potential trade-offs between resource allocation and reproductive and vegetative plant structures can challenge the response to selection for both grain and forage production under dual-purpose use. Our goal was to understand the genetic relationship between grain and forage yield components, quantify potential trade-offs between vegetative and reproductive allocation, and optimize the response to selection under dual-purpose management. Phenological, grain, and forage traits were evaluated in 30 half-sib families across two field experiments conducted over three years. No trade-offs were detected between grain and forage yield traits, indicating that the simultaneous improvement of both traits is feasible. Grain yield per spike and spikes per plant are promising secondary traits for indirect selection, given their moderate-to-high heritability (h² = 0.58 and 0.41) and strong Pearson correlation coefficients with grain yield per plant (0.68 and 0.82). These traits could be assessed in the first year, increasing genetic gain per unit time. Intermediate wheatgrass germplasm could therefore be efficiently developed by shortening the time to first evaluation, using secondary traits, and performing selection under dual-purpose management. Full article

This study investigates the performance of phenol–formaldehyde adhesives containing Eucalyptus lignin as an extender in their formulation. A commercial phenol–formaldehyde resin was used, and five different types of lignin were tested: (1) kraft lignin precipitated with carbon dioxide, (2) kraft lignin precipitated with sulfuric acid, (3) soda lignin precipitated with hydrochloric acid, (4) soda lignin precipitated with sulfuric acid, and (5) a second soda lignin where the wood underwent a phosphoric acid extraction process prior to alkaline extraction. The lignins were used both unmodified and activated through three different processes: hydroxymethylation, phenolysis in an acidic medium, and alkaline phenolysis. Adhesives were formulated with substitution percentages of the base resin ranging from 10% to 60%, in addition to a reference adhesive that contained no lignin. Wooden test specimens were manufactured to determine the tensile shear strength. Results indicate that best performance is achieved when lignins are activated through hydroxymethylation and when soda lignin is used. Under optimal conditions, it is possible to replace at least 45% of the base resin with activated Eucalyptus soda lignin, which represents a reduction of at least 30% in the cost of the final adhesive. This substitution results in a 46% increase in adhesive strength compared to the base adhesive (without lignin). These findings suggest that the valorization Eucalyptus soda lignin could have significant economic and environmental benefits. Full article