Search Results (681)

The sustainable management of balneological resources is vital for the development of eco-friendly health tourism and regional economic stability. This study presents a comprehensive physicochemical and eco-toxicological characterization of the therapeutic peloids (mud) from Lake Maly Akkol, which is located in the Zhambyl region of Kazakhstan. Utilizing an integrated approach of laboratory analysis and Python-based statistical modeling, we evaluated the resource’s clinical potential and environmental safety. The results identify the deposit as a high-quality sulfide–silt peloid with a mean humidity of 66.91% (95% CI: [65.21, 68.60]) and a mineralization level of 11.21 g/dm³ (95% CI: [10.84, 11.57]). Statistical validation using one-sample t-tests confirmed that critical therapeutic indicators, including shear strength ($\mu$ = 2593.72 dyne/cm²) and total sulfide content ($\mu$ = 0.079%), are significantly aligned with international balneological standards (p < 0.05). Eco-toxicological screening for heavy metals revealed that Lead (37.03 mg/kg) and Cadmium (0.06 mg/kg) remain well below safety thresholds, ensuring the resource’s “clean” environmental profile. These findings establish a statistically robust “Digital Quality Passport” for the Lake Maly Akkol deposit, providing the scientific foundation necessary for its sustainable industrial utilization and long-term ecological preservation. Full article

This study investigates the effects of extraction technique, solvent type, and geographical origin on the recovery of bioactive compounds from Arbutus unedo L. leaves collected from two Croatian islands (Vis and Mali Lošinj) and extracted using conventional, Soxhlet, and ultrasound-assisted extraction (UAE) with green solvents (distilled water, 70% ethanol, and ethyl acetate). Extracts were purified and characterized by thin-layer chromatography, column chromatography, and FTIR spectroscopy. Total phenols, hydroxycinnamic acids, flavonols, condensed tannins, and antioxidant capacity were quantified spectrophotometrically. Solvent type had the greatest influence, with 70% ethanol yielding the highest levels of bioactives and antioxidant capacity. Geographical origin significantly affected total phenolics and condensed tannins, with leaves from Vis outperforming those from Mali Lošinj. UAE was slightly more efficient than conventional and Soxhlet methods, particularly for thermolabile phenolics. Machine learning algorithms were applied as exploratory tools, using total phenols as a proxy variable to estimate selected bioactive compounds and antioxidant capacity based on extraction parameters. Decision Tree and Gradient Boosting models showed high goodness of fit within the experimental dataset (R² > 0.91). These results support the potential of green extraction strategies combined with data-driven screening for the valorization of A. unedo leaf extracts, while highlighting the need for further validation prior to industrial application. Full article

Khao Dawk Mali 105 (KDML 105) is a widely consumed Thai staple, but conventional cooking yields rapidly digestible starch with limited functional health benefits. This study aimed to formulate pressure-cooked KDML 105 rice as a functional food using extra–virgin coconut oil, citric acid, and Thai herbs (butterfly pea flower or pandan leaf juice). Rice was pressure-cooked, cooled at room temperature, stored at 4 °C for 24 h, and frozen at −20 °C to promote resistant starch (RS) formation. RS content increased from 0.65 g 100 g⁻¹ DW in control rice to 1.39 g 100 g⁻¹ DW with coconut oil, and to 2.08 and 1.80 g 100 g⁻¹ DW when citric acid plus pandan or butterfly pea juice were added, respectively. Coconut oil-treated samples showed higher antioxidant activity in DPPH and FRAP assays, while formulations with butterfly pea or pandan juices additionally reduced ABTS^•+ radicals. Prebiotic potential was evaluated in vitro using Levilactobacillus brevis, Lactobacillus bulgaricus, and Streptococcus thermophilus grown in MRS medium with rice extracts. All formulations enhanced probiotic growth versus control, indicating that this pressure cook–cool process can produce a ready-to-eat functional rice with improved RS, antioxidant capacity, and probiotic support. Full article

Obtaining high-purity ⁹⁰Sr is crucial because it is the parent radionuclide of the ⁹⁰Sr/⁹⁰Y generator. However, ⁹⁰Sr products recovered from high-level liquid waste (HLLW) often fail to meet the stringent purity requirements. This necessitates the development of a novel extraction system that can seamlessly connect with existing separation processes to achieve the required purity level. A novel diglycolamide (DGA) ligand was designed and synthesized. The distribution ratios (D) of several traditional organic diluents and ionic liquids (ILs) as diluents were compared under the same experimental conditions; 1-butyl-3-methylimidazolium bis(trifluoromethanesulphonyl)imide ([C₄mim][NTf₂]) was chosen as the optimal diluent. The HNO₃ concentration, ligand concentration, [C₄mim]⁺ concentration, etc., were assessed. The extraction mechanism was confirmed to ensure that the extraction proceeded mainly via the [C₄mim]⁺ and H⁺ exchange mechanisms. Slope analysis and the ESI-MS results revealed that the novel ligand N,N-diphenyl-N′,N′-dibutyl diglycolamide (DPDBDGA, L) in [C₄mim][NTf₂] formed a 1:3 complex with Sr²⁺. The experiments on Sr²⁺ indicated that it can be recovered completely with 1 M mineral acid within two stages. Furthermore, we predicted that the novel DGA ligand would provide a good extraction capacity for Sr²⁺ in dilute nitric acid in the [C₄mim][NTf₂] system. This system can be linked to the separation process of extracting Sr²⁺ from HLLW using N,N,N′,N′-tetraoctyl-diglycolamide (TODGA) or crown ethers as extractants. Consequently, high-purity ⁹⁰Sr products can be obtained. Full article

Mpox is a zoonosis caused by the monkeypox virus. Here, we report Mali’s index Mpox case, which was clinically identified at the Mali–Guinea border by the national telemedicine center and confirmed by PCR. The library prepared with NextGenPCR™ MPXV Sequencing Library Prep and sequenced on Minion MK1C revealed a genome length of 197,122 bp with an average depth of 1284.4×. The strain belonged to Clade IIb G1 lineage and exhibited 85 mutations relative to NC_063383.1. To decipher genomic epidemiology, genomes ≥ 195 kb were retrieved from NCBI and aligned with MAFFT. Time-resolved phylogenetic reconstruction and ancestral trait inference were performed with TreeTime v0.11.4. A median joining network was built with Popart v1.7. Phylogeographic analysis revealed clustering with Clade IIb (G.1 lineage) linked to the May 2025 outbreak in Sierra Leone. Full article

Edible Bird’s Nest (EBN) is a highly prized food product, making it a frequent target for economic adulteration. Consequently, robust quality assurance is paramount to protect consumers and ensure market integrity. A significant barrier to effective quality control, however, is an incomplete understanding of the natural chemical variability within authentic EBN. This variability, influenced by factors such as geographical origin, bird species, and post-harvest processing, can confound analytical measurements and complicate the definition of a standard reference. This study provides an existence proof in a defined cohort, characterizing microscale chemical heterogeneity in authentic A. fuciphagus EBN. We employed a multi-modal Fourier Transform Infrared (FTIR) spectroscopy approach, integrating transmission, macro-attenuated total reflectance (ATR), and high-resolution micro-ATR chemical imaging. A diverse set of validated, authentic EBN samples was analyzed using unsupervised Principal Component Analysis (PCA) to explore the data structure. Our results reveal significant and previously unquantified spectral heterogeneity, particularly in protein and glycoprotein-related regions. In our cohort, the chemical signatures of authentic EBN do not collapse to a single, uniform profile but span a broad, multi-dimensional continuum. This inherent variability presents a critical challenge for conventional quality control methods that rely on simplistic, single-spectrum standards, which may lead to the misclassification of genuine products. By establishing a robust chemical baseline for the authentic class, this work provides the foundational data essential for developing next-generation authentication models capable of reliably distinguishing this natural variance from deliberate adulteration. Full article

The sour cherry (Prunus cerasus L.) is an important fruit species in Eastern Europe due to its multiple uses. The aim of this study was to evaluate the ripening-stage-dependent compositional changes in some Hungarian-bred sour cherry varieties (“amarelle” type ‘Korai pipacs’, “morello” types ‘Érdi bőtermő’ and ‘Újfehértói fürtös’), with a special view on biologically active compounds (anthocyanins, polyphenols, vitamin C, melatonin), organic acids, sugars, and antioxidant characteristics. The measured soluble solid content varied within a narrower range than reported in the literature, whereas the total acid content and soluble solid content were consistent with previous data. As the analyses were based on samples from a single year (2022), potential year-to-year variability should be considered when interpreting the results. The “morello” type varieties reached a higher amount of different sugar compounds than the “amarelle” type variety. Among the examined organic acid compounds, malic acid was detected in the highest quantity (176.75 to 669.44 mg 100 mL⁻¹). The vitamin C data (5.74 to 13.46 mg 100 mL⁻¹) had similarity to the literature data. The “amarelle” type ‘Korai pipacs’ reached the highest antioxidant content (131.11 mM AS L⁻¹) in the third picking time. Among the naturally occurring pigments the “morello” type, straining sour cherries reached a higher amount (113.71 µg mL⁻¹ in ‘Érdi bőtermő’ and 59.4 µg mL⁻¹ in ‘Újfehértói fürtös’ of cyanidin glucosides), than the observed “amarelle” type (23.42 µg mL⁻¹ in ‘Korai pipacs’ of cyanidin glucosides). Melatonin was detected in all examined varieties (1.56 to 13.25 ng mL⁻¹). Full article

Endophytic fungi represent an extensive source of chemically diverse and bioactive polyketides. Herein, an HSQC-based DeepSAT-guided strategy was employed for the scaffold-prioritized isolation of metabolites from Diaporthe kyushuensis ZMU-48-1, an endophyte isolated from Acacia confusa. DeepSAT analysis of the semi-purified fractions identified an α-pyrone chemotype, which facilitated the targeted isolation of thirteen pyranone-type polyketides, including eight previously undescribed analogues, diaporthopyranone A–H (1–8). The structures of the new compounds were rigorously elucidated via comprehensive spectroscopic analysis (1D and 2D NMR and HRESIMS), while their absolute configurations were established by comparing experimental and TDDFT-calculated ECD data. All isolates (1–13) were screened for antifungal activity against nine plant pathogens. While most metabolites were inactive at 200 μg/mL, compound 4 inhibited Alternaria alternata and Valsa mali (MICs = 100 and 80 μg/mL, respectively), and compounds 6 and 8 exhibited selective inhibition against Colletotrichum musae (MICs = 60 and 80 μg/mL). These results expand the pyranone repertoire of the genus Diaporthe and underscore the utility of the HSQC–DeepSAT platform for the streamlined discovery of specific natural product scaffolds. Full article

We discuss the geometry behind the classical Heisenberg model at the level suitable for third- or fourth-year students who did not have the opportunity to take a course on differential geometry. The arguments presented here rely solely on elementary algebraic concepts such as vectors, dual vectors and tensors, as well as Hamiltonian equations and Poisson brackets in their simplest form. We derive Poisson brackets for classical spins, along with the corresponding equations of motion for the classical Heisenberg model, starting from the two-sphere geometry, thereby demonstrating the relevance of standard canonical procedures in the case of the Heisenberg model. Full article

Due to the relatively long lifespan and resilience of adults to environmental stressors, freshwater turtles are characterized as bioaccumulators of chronic contaminant exposure in urban ecosystems. Urbanization increases pollutants, resulting in subsequent runoff into streams. We evaluated the relationship between percent impervious surface and contaminant concentrations in turtles from 20 wetlands in Wake County, North Carolina, USA, one of the fastest-growing counties in the United States. We evaluated the concentrations of eight environmental contaminants known to cause human and environmental health issues listed under the U.S. Environmental Protection Agency’s Resource Conservation and Recovery Act: arsenic (As), barium (Ba), cadmium (Cd), chromium (Cr), lead (Pb), mercury (Hg), selenium (Se), and silver (Ag), as well as vanadium (V) and copper (Cu) due to their presence in urban environments and bioaccumulation, in the blood and claws from Chelydra serpentina and Trachemys scripta. All contaminants, except for Cd and Ag, were detected in both species and both tissue types. Carnivorous Chelydra serpentina exhibited higher concentrations of Se and Hg than omnivorous Trachemys scripta. Partial redundancy analysis indicated that species accounted for more variance in the data than % impervious surface at a 2200-m scale. Robust mixed-effects models showed that % impervious surface was not correlated with contaminant concentrations in either species. Although we documented no relationship between urbanization and contaminant concentrations, we recommend additional research to investigate the effects of urbanization over time in this rapidly developing region. Full article

Agriculture serves as a critical foundation for livelihoods, food security, and sustainable development across the Sahara–Sahelian region. However, this vital sector faces mounting pressures from recurrent armed conflicts that systematically undermine its resilience and long-term sustainability. This study provides a comprehensive analysis of agricultural technical efficiency across 23 African countries in the Sahara–Sahelian region from 2009 to 2021, employing a robust bias-corrected bootstrap Data Envelopment Analysis approach. The findings reveal a concerning regional deterioration, with technical efficiency declining at an average annual rate of 1.7% throughout the study period. Conflict-affected countries demonstrated distinctive vulnerability patterns, exhibiting both higher average efficiency levels (0.875) and greater volatility, with annual declines of 1.8%. Sub-regional analysis highlights the Sahel’s particular fragility, where efficiency decreased by 2.2% yearly, nearly double the decline rate observed in North Africa. The most severe efficiency losses were recorded in countries experiencing intense and protracted conflict, notably Burkina Faso (4.0%) and Mali (3.5%), underscoring the severe association between conflict exposure and the erosion of agricultural productive capacity. These findings underscore the importance of developing integrated strategies that simultaneously address security challenges, climate adaptation, and institutional reform for effective resilience-building. Policy recommendations highlight the importance of enhanced regional connectivity, knowledge transfer, and targeted investments in agricultural capacity building—all aligned with both Sustainable Development Goals and the African Union’s Agenda 2063 objectives for achieving sustainable agricultural transformation in conflict-affected regions. Full article

Background: Extrahepatic portal vein obstruction (EHPVO) accounts for more than two thirds of pediatric portal hypertension. Rex shunt is the preferred surgical management, as it restores hepatopetal flow and minimizes or reverses liver dysfunction. Case Summary: We report surgical treatment of EHPVO in a 9-year-old girl using portal-Rex shunt with internal jugular vein (IJV) as a conduit and the intact proximal main portal vein instead of the superior mesenteric vein as a venous inlet. Improvement in thrombocytopenia and reduction in splenic size were achieved post-operatively. The portal-Rex shunt remains patent with good hepatopetal flow at one year post-operation. Conclusions: The success of a portal-Rex shunt to selectively bypass EHPVO rests upon careful selection of inlet and outlet veins, as well as a conduit with good patency, such as the IJV. Full article

Background/Objectives: Triple-negative breast cancer (TNBC) is an aggressive subtype associated with a poor prognosis and limited treatment options. Inflammatory and hematological biomarkers have emerged as potential tools for disease characterization, particularly in low-resource settings. Methods: This cross-sectional analytical study was conducted between July 2022 and February 2024 at Dalal Jamm Hospital in Dakar, Senegal, and included 120 women: 40 with TNBC, 40 with hormone-dependent breast cancer (HDBC), and 40 healthy controls. Blood samples were collected at diagnosis before any treatment to measure complete blood counts and C-reactive protein (CRP) levels. Inflammatory ratios—neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR), and platelet-to-lymphocyte ratio (PLR)—were calculated. Results: TNBC patients displayed a distinct inflammatory profile characterized by elevated neutrophil counts, CRP, NLR, and MLR, as well as reduced lymphocyte and basophil percentages compared to healthy controls. NLR > 1.12 demonstrated strong discriminatory ability (AUC = 0.847; sensitivity 90%; specificity 65%). Differences between TNBC and HDBC were less pronounced, except for CRP and basophil levels. Multivariate analysis confirmed independent associations of elevated NLR, CRP, and neutrophils with TNBC. Conclusions: These findings provide new insights into the inflammatory and hematological characteristics of TNBC in this population and support further investigation of accessible biomarkers for early disease stratification in similar settings. Full article

The objective of this study was to investigate the effect of reactive extrusion (thermomechanical and chemical process) on the chemical composition, techno-functional properties, glucose and cholesterol adsorption capacity, and bioactive compound profile of spent coffee grounds (SCG). SCG was extruded using citric acid or alkaline hydrogen peroxide as reagents, and a control sample was extruded without reagents. Treatment with citric acid resulted in the highest levels of total dietary fiber (79.6 g/100 g) and insoluble fiber (76.2 g/100 g), especially cellulose, and significantly improved glucose (32.7 mmol/L) and cholesterol (4.5 mg/g) adsorption at neutral pH. Treatment with alkaline hydrogen peroxide increased water retention capacity (3.9 g/g). Although chemical treatments reduced total polyphenol and antioxidant activity, they effectively broke down the lignocellulosic matrix, thereby increasing fiber availability and functionality. Extrusion without reagents (processes induced by mechanical and thermal factors) favored the retention of caffeine and chlorogenic acids, increasing soluble fiber and maintaining antioxidant capacity. Therefore, reactive extrusion is a technological strategy that aligns with the principles of the circular economy, offering an environmentally friendly alternative to landfill disposal and adding value to spent coffee grounds by transforming lignocellulosic residue into functional ingredients with broad application potential. Full article

Valsa canker, caused by Valsa mali (Cytospora mali) or V. pyri, is a destructive fungal disease affecting apple and pear production. NAC transcription factors have been shown to participate in multiple immune signaling pathways. However, the key members of this family involved in Valsa canker resistance remain largely unknown. In this study, we demonstrate that PbeNAC72, an NAC transcription factor responsive to Valsa canker signals in Pyrus betulifolia, positively regulates resistance in apple and pear fruits. In ‘Duli-G03’ (P. betulifolia) suspension cells, overexpression of PbeNAC72 significantly enhanced resistance to Valsa canker and induced reactive oxygen species bursts, as well as the expression of salicylic acid and jasmonic acid-related genes. Subsequent weighted gene co-expression network analysis revealed that PbeNAC72 is primarily linked to genes associated with “DNA replication” and “microtubule-based movement.” We therefore suggest that PbeNAC72 activates genes related to core signaling pathways and fundamental cellular processes, which further contribute to the positive regulation of Valsa canker resistance. Our results provide novel insights into the resistance mechanism and identify a candidate gene for future molecular breeding efforts. Full article